Resumo Abordagens conservadoras, incluindo o uso de placas oclusais, são as primeiras opções de tratamento para disfunção temporomandibular (DTM), devido à sua reversibilidade. O presente estudo analisou o efeito da posição do disco articular e do uso de placa oclusal na distribuição de tensões no disco. Um modelo bidimensional (2D) de articulação temporomandibular (ATM) em posição fisiológica, foi desenvolvido para análise pelo método de elementos finitos, baseado em imagens de tomografia computadorizada do tipo cone-beam. Três outros modelos foram criados alterando a posição do disco de acordo com o uso de placa oclusal e condição de deslocamento anterior do disco. Uma análise estrutural da distribuição de tensões foi realizada no software Marc-Mentat, e equivalente Von Mises foi usado para comparar os fatores em estudo. Maior concentração de tensão foi observada na zona intermediária para a zona anterior do disco, atingindo valores máximos acima de 2 MPa. Nenhuma diferença relevante foi verificada na localização e magnitude das tensões quando comparadas as posições do disco e uso de placa oclusal. No entanto, houve pequena redução das tensões decorrente do uso de placa oclusal em caso de deslocamento anterior do disco. Conclui-se que mediante os modelos criados e condições de contorno estabelecidas, as tensões na zona intermediária do disco aumentam durante o fechamento mandibular fisiológico. A magnitude das tensões foi semelhante em todas as situações testadas

Abstract Conservative approach, including occlusal splint therapy, is the first option to treat temporomandibular disorders (TMD), because of its reversibility. The present study analyzed the effect of the articular disc position and occlusal splints use on the stress distribution on this disc. A two-dimensional (2D) finite element (FE) model of the temporomandibular joint with the articular disc at its physiologic position was constructed based on cone-beam computed tomography. Three other FE models were created changing the disc position, according to occlusal splint use and anterior disc displacement condition. Structural stress distribution analysis was performed using Marc-Mentat package. The equivalent von Mises stress was used to compare the study factor. Higher stress concentration was observed on the intermediate to anterior zone of the disc, with maximum values over 2MPa. No relevant difference was verified on the stress distribution and magnitude comparing disc positions and occlusal splint use. However, there was stress reduction arising from the use of the occlusal splints in cases of anterior disc displacement. In conclusion, based on the generated FE models and established boundary conditions, the stress increased at the intermediate zone of the TMJ disc during physiological mandible closure. The stress magnitude was similar in all tested situations

To evaluate the association between temporomandibular disorder (TMD) and anxiety, quality of sleep, and quality of life in nursing professionals at theHospital de Clínicas de Uberlândiaof theUniversidade Federal de Uberlândia – HCU-UFU (Medical University Hospital of the Federal University of Uberlândia), four questionnaires were given to nursing professionals. The questionnaires were completed by 160 of these professionals. The Fonseca’s questionnaire was used to evaluate the presence and severity of TMD, the IDATE was used to evaluate anxiety, the SAQ was used to evaluate quality of sleep, and the SF-36 was used to evaluate quality of life. Forty-one nurses (25.6%) reported having no TMD (Fonseca’s questionnaire score ≤ 15), 66 (41.3%) had mild TMD (Fonseca’s questionnaire score 20–40), 39 (24.4%) had moderate TMD (Fonseca’s questionnaire score 45–65), and 14 (8.8%) had severe TMD (Fonseca’s questionnaire score ≥ 70). According to Fonseca’s questionnaire, the presence of TMD was associated with trait anxiety, but the TMD severity was associated with state anxiety classification (mild, moderate, severe). The SAQ score differed significantly from Fonseca classification. The Fonseca’s questionnaire score correlated negatively with the score of each dimension of the SF-36 (r = –0.419 to –0.183). We conclude that TMD is common among nursing professionals; its presence was associated with trait anxiety, and its severity was associated with state anxiety. Hence, the presence of TMD may reduce quality of sleep and quality of life.

Aim: To investigate the prevalence of temporomandibular disorders (TMD) in undergraduate students and to correlate its prevalence with occlusal factors and parafunctional habits. Methods: 201 undergraduate students were evaluated. The Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) was filled out at the beginning of the study, followed by occlusal analysis based on morphological and functional alterations. The identification of tooth grinding and clenching was carried out by self-reports. Statistical analysis was based on chi-square and multivariate logistic regression analyses. p-value for all statistical analysis was set at 5%. Results: According to RDC/TMD, 18.4% of subjects experienced myofascial pain (G-MPD), and 12.4% had joint disorder with disc displacement (G-DD). Tooth clenching was statistically associated with TMD (p=0.000). In the occlusal factors, overjet showed statistically significant correlation only with myofascial pain. No association between functional alteration and TMD was found. No statistically significant correlation was found between G-DD and occlusal alterations or parafunctional habits. Conclusions: Overjet and tooth clenching were correlated with G-MPD. Occlusal alterations or parafunctional habits did not show correlation with G-DD.

O objetivo deste estudo foi avaliar a capacidade de identificação de prematuridades oclusais por meio de imagens obtidas de cortes paraxiais de tomografias computadorizadas de feixe cônico. Para este fim, realizou-se um estudo piloto em que 16 pacientes jovens assintomáticos foram submetidos ao exame clínico, incluindo análise oclusal criteriosa, e em seguida foram confeccionados dispositivos desprogramadores individuais (“JIG de Lucia”). Mediante manipulação mandibular e marcação interoclusal com papel carbono (Accufilm) identificou-se clinicamente o contato prematuro em relação cêntrica (RC) de cada paciente. Posteriormente estes dispositivos foram ajustados na posição de contato cêntrico e utilizados durante os exames tomográficos para que se pudessem obter imagens tomográficas na posição de relação cêntrica. Após o processamento das imagens, estas foram analisadas com o intuito de identificar prematuridades oclusais nas imagens visualizadas por 30 profissionais divididos de acordo com as áreas de atuação (Oclusão, Clínica Geral e Radiologia, n=10 para cada área) e tempo de exercício profissional (<5; entre 5 e 10; e >10 anos). Mediante comparação entre os contatos prematuros identificados na análise clínica oclusal e as imagens tomográficas apontadas pelos profissionais como áreas de contatos prematuros, foram calculados os índices de concordância entre essas duas variáveis. Os dados obtidos foram analisados estatisticamente pela Análise de Variância ANOVA e teste de Scott-Knott (α=0,05). Os resultados deste estudo demonstraram que a identificação de prematuridades oclusais por meio de cortes paraxiais de tomografias computadorizadas de feixe cônico mostrou-se como um método de confiabilidade média.

The aim of this study was to evaluate the ability to identify occlusal prematurity by images from paraxial slices of cone-beam computed tomography (CBCT). For such purpose, a pilot study was performed in which 16 asymptomatic young patients were subjected to a clinical examination, including a careful occlusal analysis and then individual deprogrammer devices (“Lucia's JIG”) were fabricated. Premature contacts were clinically identified in centric relation (CR) for each patient by jaw manipulation and interocclusal marking with articulating paper (Accufilm). Subsequently, these devices were adjusted in CR and used during the tomographic exams in such a way that CBCT in CR could be obtained. After routine processing, the images were analyzed in order to identify occlusal prematurity on the displayed images by 30 professionals divided according to areas of activity (occlusion specialist, general practitioner and radiologist; n=10 per area) and time of professional experience (less than 5, between 5 and 10, and over 10 years). By comparing the premature contacts identified in the clinical analysis and CBCT images, an agreement index between these two variables was calculated. Data were analyzed statistically by ANOVA and Scott-Knott test (α=0.05). The results showed that the identification of occlusal prematurity by paraxial CBCT slices was proven to be a method of average reliability.

O leite de soja (LS) é largamente consumido em todo o mundo como substituto para o leite bovino. Este é uma fonte de vitaminas, carboidratos e açúcares, mas a sua capacidade para preservar a viabilidade celular não foi avaliada. A finalidade do estudo foi investigar a eficácia do LS em manter a viabilidade de fibroblastos humanos em períodos curtos em comparação com diferentes leites bovinos. Fibroblastos de boca humanos foram cultivados e armazenados nos seguintes meios à temperatura ambiente: 10% de meio Dulbecco's Modified Eagle (DMEM) (grupo controle positivo); leite bovino integral longa vida (LI); leite bovino desnatado longa vida - LD; leite em pó - LP; leite de soja - LS. Depois de 5, 15, 30 e 45 min, a viabilidade celular foi analisada usando o método de MTT. Os dados foram analisados estatisticamente pelo teste de Kruskal-Wallis e posteriormente usando o método de Dunn (α=0,05). O grupo LD apresentou a menor capacidade para manter a viabilidade celular em todos os tempos analisados (p<0,05). Aos 30 e aos 45 min, os níveis de absorbância no grupo controle (DMEM) e LS foram significativamente maiores que no grupo LD (p<0,05). A viabilidade celular diminuiu ao longo do tempo (5-45 min). Os resultados indicaram que LS pode ser usado como meio de armazenamento mais adequado para dentes avulsionados. LD não foi eficaz na preservação da viabilidade das células como o meio de cultura de células e o LS.

Soy milk (SM) is widely consumed worldwide as a substitute for cow milk. It is a source of vitamins, carbohydrates and sugars, but its capacity to preserve cell viability has not been evaluated. The purpose of the present study was to investigate the efficacy of SM to maintain the viability of human fibroblasts at short periods compared with different cow milks. Human mouth fibroblasts were cultured and stored in the following media at room temperature: 10% Dulbecco's Modified Eagle Medium (DMEM) (positive control group); long shelf-life ultra-high temperature whole cow milk (WM); long shelf-life ultra-high temperature skim cow milk (SKM); powdered cow milk (PM); and soy milk (SM). After 5, 15, 30 and 45 min, cell viability was analyzed using the MTT assay. Data were analyzed statistically by the Kruskal-Wallis test with post-analysis using the Dunn's method (α=0.05). SKM showed the lowest capacity to maintain cell viability in all analyzed times (p<0.05). At 30 and 45 min, the absorbance levels in control group (DMEM) and SM were significantly higher than in SKM (p<0.05). Cell viability decreased along the time (5-45 min). The results indicate that SM can be used as a more adequate storage medium for avulsed teeth. SKM was not as effective in preserving cell viability as the cell culture medium and SM.

Este estudo avaliou o efeito de diferentes tipos de dispositivos de aplicação de carga na resistência à fratura de pré-molares superiores restaurados com resina composta. Sessenta pré-molares humanos receberam preparo mésio-ocluso-distal padronizado e as cavidades foram restauradas com resina composta. As amostras foram aleatoriamente divididas em 6 grupos (n=10). Carregamento de compressão foi realizado usando seis diferentes dispositivos metálicos de aplicação de carga: S2 e S6: esfera de 2 mm e 6 mm de diâmetro respectivamente; C2 e C6: cilindro de 2 mm e 6 mm de diâmetro respectivamente; WS: lâmina de faca e MAT: antagonista individualizado em metal. Os resultados foram submetidos à análise de variância e teste de Tukey (p<0,05). O modo de falha foi classificado em 4 níveis e estão apresentado em porcentagem. O dispositivo WS resultou em maior resistência a fratura de pré-molares que S6 e C6. Não houve diferença estatística entre os grupos MAT, C2, S2 e S6. Menor resistência foi verificada com o uso de S6 e C6. O dispositivo MAT resultou em menor número de falhas catastróficas enquanto nos grupos C2, S2 e WS essas falhas foram significativas. O tipo de dispositivo de aplicação de carga influenciou significativamente no comportamento do complexo dente-restauração durante o teste de resistência à fratura.

This in vitro study analyzed the effect of different load application devices on fracture resistance and failure mode of maxillary premolars restored with composite resin. Sixty human maxillary premolars received standardized mesio-occluso-distal cavity preparations and were restored with composite resin. The specimens were randomly divided into 6 groups (n=10). Compressive loading was applied using 6 different metallic devices: S2: 2-mm sphere; S6: 6-mm sphere; C2: 2-mm cylinder; C6: 6-mm cylinder; WS: wedge shape device; and MAT: individualized metallic antagonist tooth. Data were analyzed statistically using one-way ANOVA and Tukey's test (α=0.05). The failure mode was recorded based on the 4 sequential levels. Statistical analysis revealed that WS presented significantly higher fracture resistance than S6 and C6. No significant difference was found among MAT, C2, S2 and S6. Sphere and cylinder with 6 mm were similar, with the lowest values of all groups. MAT presented the least number of catastrophic failures while C2, S2 and WS presented the highest. The type of load application device influences significantly the behavior of the teeth-restoration complex during mechanical fracture resistance test.

The magnitude of occasional discrepancies between the centric relation and maximal intercuspation positions remains a controversial subject. This study quantitatively evaluated the possible discrepancies in the condyle/mandibular fossa relationship between these positions using cone-beam computed tomography. Twenty young and asymptomatic volunteers were distributed equally into normal occlusion and Angle Class I, II and III malocclusion groups. They were submitted to one tomographic scan in maximal intercuspation and one in centric relation. Measurements were performed on lateral and frontal cuts of the patients' temporomandibular joints, and the data collected were compared using Student's t test at a significance level of 5%. The results showed that there were no statistically significant differences between the centric relation and maximal intercuspation positions in young and asymptomatic patients with practically intact dentitions using cone-beam computed tomography.

O objetivo deste estudo foi avaliar o efeito de diferentes parâmetros de solda a plasma verificando a resistência flexural de barras de liga de titânio (Ti-6Al-4V). Quarenta espécimes de Ti-6AL-4V e dez espécimes de liga de NiCr foram preparados (40 mm de altura e 3,18 mm de diâmetro) e divididos em 5 grupos (n=10). As barras de liga titânio do grupo controle não foram seccionadas e nem submetidas a processo de soldagem. Os grupos PL10, PL12 e PL14, continham barras de titânio seccionadas e soldadas com uma corrente de 3 A por períodos de 10,12 e 14 ms, respectivamente. O grupo NCB consistiu de barras de liga de NiCr soldadas pelo método de brasagem convencional. Em seguida as barras foram submetidas ao teste de flexão de três pontos e os valores obtidos foram transformados em resistência flexural (MPa). Análise estatística foi realizada usando ANOVA de fator único e o teste Tukey com o nível de significância de 0,05. Diferença estatística foi verificada entre os grupos avaliados (p<0,001), com os maiores valores pra o grupo controle. Não foram encontradas diferenças entre os grupos de solda plasma (p>0,05). O grupo NCB apresentou os menores valores de resistência flexural embora tenha sido estatisticamente semelhante ao grupo PL14. Não houve diferença significante na profundidade de penetração entre os grupos de solda plasma (p=0,05). Três espécimes representativos foram aleatoriamente selecionados para serem avaliados por meio de microscopia eletrônica de varredura. A composição das regiões soldadas foi verificada por meio de espectroscopia por energia dispersiva. Este estudo fornece informações iniciais de parâmetros para permitir a utilização de soldagem plasma durante a fabricação de estruturas em liga de titânio.

The aim of this study was to assess the effect of different plasma arc welding parameters on the flexural strength of titanium alloy beams (Ti-6Al-4V). Forty Ti-6Al-4V and 10 NiCr alloy beam specimens (40 mm long and 3.18 mm diameter) were prepared and divided into 5 groups (n=10). The titanium alloy beams for the control group were not sectioned or subjected to welding. Groups PL10, PL12, and PL14 contained titanium beams sectioned and welded at current 3 A for 10, 12 or 14 ms, respectively. Group NCB consisted of NiCr alloy beams welded using conventional torch brazing. After, the beams were subjected to a three-point bending test and the values obtained were analyzed to assess the flexural strength (MPa). Statistical analysis was carried out by one-way ANOVA and Tukey's HSD test at 0.05 confidence level. Significant difference was verified among the evaluated groups (p<0.001), with higher flexural strength for the control group (p<0.05). No significant differences was observed among the plasma welded groups (p>0.05). The NCB group showed the lowest flexural strength, although it was statistically similar to the PL 14 group (p>0.05). The weld depth penetration was not significantly different among the plasma welded groups (p=0.05). Three representative specimens were randomly selected to be evaluated under scanning electron microcopy. The composition of the welded regions was analyzed by energy dispersive X-ray spectroscopy. This study provides an initial set of parameters supporting the use of plasma welding during fabrication of titanium alloy dental frameworks.

A adição de diferentes polímeros como o politetrafluoretileno (PTFE) às resinas para base de próteses removíveis pode ser uma opção para modificar as propriedades mecânicas das resinas acrílicas. Este estudo avaliou a dureza de superfície, a resistência ao impacto e à flexão, o módulo flexural e a carga de ruptura de duas resinas acrílicas, uma submetida ao ciclo longo e a outra ao ciclo curto de polimerização, as quais foram preparadas com ou sem a adição de PTFE a 2%. Quatro grupos foram formados de acordo com os ciclos de polimerização e a adição ou não de PTFE. Quarenta espécimes foram preparados para cada teste (10 espécimes por grupo) com as seguintes dimensões: dureza de superfície (30 mm diametro x 5 mm espessura), resistência ao impacto (50 x 6 x 4 mm), resistência à flexão (64 x 10 x 3,3 mm). O módulo flexural e a carga de ruptura foram calculados a partir dos valores obtidos no teste de resistência à flexão. Todos os testes foram realizados em conformidade com a Norma ISO 1567:1999. Os dados foram submetidos à análise de variância e teste de Tukey com nível de significância de 5%. Não foram encontradas diferenças estatisticamente significantes (p>0,05) para dureza de superfície. Os valores de resistência à flexão, resistência ao impacto e carga de ruptura foram maiores para as resinas sem a adição de PTFE (p<0,05). O valor do módulo flexural da resina acrílica com adição de PTFE a 2%, polimerizada por ciclo longo, foi maior do que as outras resinas (p<0,05). Dentro das limitações deste estudo, pode-se concluir que a adição de politetrafluoretileno não melhorou as propriedades mecânicas das resinas acrílicas avaliadas.

The addition of different polymers, such as polytetrafluorethylene (PTFE), to denture base resins could be an option to modify acrylic resin mechanical properties. This study evaluated the surface hardness, impact and flexural strength, flexural modulus and peak load of 2 acrylic resins, one subjected to a long and another subjected to a short polymerization cycle, which were prepared with or without the addition of 2% PTFE. Four groups were formed according to the polymerization cycle and addition or not of PTFE. Forty specimens were prepared for each test (10 per group) with the following dimensions: hardness (30 mm diameter x 5 mm thick), impact strength (50 x 6 x 4 mm) and flexural strength (64 x 10 x 3.3 mm) test. The results of the flexural strength test allowed calculating flexural modulus and peak of load values. All tests were performed in accordance with the ISO 1567:1999 standard. Data were analyzed statistically by ANOVA and Tukey's test with the level of significance set at 5%. No statistically significant differences (p>0.05) were found for surface hardness. Flexural strength, impact strength and peak load were significantly higher (p<0.05) for resins without added PTFE. The flexural modulus of the acrylic resin with incorporated 2% PTFE polymerized by long cycle was significantly higher (p<0.05) than that of the other resins. Within the limits of this study, it may be concluded that the addition of PTFE did not improve the mechanical properties of the evaluated acrylic resins.

O objetivo desse estudo foi avaliar a influência de diferentes terapias periodontais na integridade de restaurações indiretas por meio de microscopia eletrônica de varredura (MEV). Sessenta raízes bovinas foram selecionadas e aleatoriamente divididas em 12 grupos. Preparos cavitários inlay foram realizados na região cervical central da junção cemento-esmalte e restaurados com resina laboratorial cimentado com cimento resinoso. Doze terapias periodontais foram usadas (n=5): C-sem tratamento (controle); MS-raspagem manual com cureta Gracey; US-raspagem com ultra-som; PP- profilaxia com pasta profilática; PS-profilaxia com pedra pomes; SBJ-jato de bicarbonato; MS/PP; MS/PS; MS/SBJ; US/PP; US/PS; US/SBJ. As amostras foram preparados e submetidas a MEV. Imagens, de ×100 a ×1000, da superfície da resina laboratorial e da interface das restaurações indiretas foram obtidas. As imagens foram avaliadas em procedimento cego por 3 observadores habilitados previamente calibrados seguindo os seguintes critérios: ranhuras, microcavidades e fratura de margem. Os resultados mostraram que a profilaxia com pedra pomes promoveu ranhuras na superfície das restaurações. Raspagem manual e ultrasônica promoveram ranhuras e fratura de margem das restaurações. Jato de bicarbonato resultou em degradação de cimento resinoso. Os resultados sugeriram que, exceto a profilaxia com pasta profilática, todos os procedimentos resultaram em efeitos deletérios na integridade da margem das restaurações indiretas.

The aim of this study was to evaluate the influence of different periodontal therapies on the integrity of indirect restorations by scanning electron microscopy (SEM). Sixty single-rooted bovine teeth were selected and randomly assigned to 12 groups. Inlay cavities were prepared in the cervical region on the center of the cementoenamel junction, and were restored with laboratory processed resin and luted with a resin cement. Twelve periodontal therapies were used (n= 5): C: control (no treatment); MS: manual scaling with Gracey curettes; US: ultrasonic scaling; PP: prophylaxis with prophylactic paste; PS: prophylaxis with pumice-stone; SBJ: sodium bicarbonate jet; MS/PP; MS/PS; MS/SBJ; US/PP; US/PS; US/SBJ. The specimens were prepared and analyzed by SEM. SEM micrographs at ×100 to ×1000 magnification were obtained from the surface of the laboratory resin and the interface of the indirect restorations. The images were evaluated by 3 skillful, calibrated, blinded observers as to the presence of grooves, microcavities and fracture of margins. The results showed that PS produced groves on restoration surface. MS and US produced groves and marginal fractures on the restorations. SBJ resulted in resin cement degradation. These results suggest that except for PS, all procedures had deleterious effects on the marginal integrity of indirect restorations.

OBJECTIVES: Alterations in the temporomandibular complex can reflect in adaptations of the individual's entire muscular system, intervening with the head position and scapular waist, developing postural alterations and modifying all corporal biomechanics. The aim of this study was to evaluate the head position (HP) and head postural alterations before and after installation of occlusal splints. MATERIAL AND METHODS: Twenty patients with temporomandibular disorders (TMD) underwent clinical and postural examination, before the installation of an occlusal splint, and after 1 week and 1 month of use. RESULTS: There were statistically differences for HP, between the initial values and after 1 week of use of the occlusal device (p= 0.048) and also between 1 week and 1 month of evaluation (p= 0.001). Decrease of the painful symptomatology and maintenance of the rectification were also observed. CONCLUSIONS: The individual's postural position can suffer biomechanical alterations due to stomatognathic alterations, causing clinically visible changes in dysfunctional individuals and affecting the performance of the involved structures.

This research consisted of a quantitative assessment, and aimed to measure the possible discrepancies between the maxillomandibular positions for centric relation (CR) and maximum intercuspation (MI), using computed tomography volumetric cone beam (cone beam method). The sample of the study consisted of 10 asymptomatic young adult patients divided into two types of standard occlusion: normal occlusion and Angle Class I occlusion. In order to obtain the centric relation, a JIG device and mandible manipulation were used to deprogram the habitual conditions of the jaw. The evaluations were conducted in both frontal and lateral tomographic images, showing the condyle/articular fossa relation. The images were processed in the software included in the NewTom 3G device (QR NNT software version 2.00), and 8 tomographic images were obtained per patient, four laterally and four frontally exhibiting the TMA's (in CR and MI, on both sides, right and left). By means of tools included in another software, linear and angular measurements were performed and statistically analyzed by student t test. According to the methodology and the analysis performed in asymptomatic patients, it was not possible to detect statistically significant differences between the positions of centric relation and maximum intercuspation. However, the resources of cone beam tomography are of extreme relevance to the completion of further studies that use heterogeneous groups of samples in order to compare the results.

This study evaluated the influence of the method and period of storage on the adhesive bond strength of indirect composite resin to bovine dentin. Ninety bovine incisors were stored in three different solutions: 0.2% thymol, 10% formalin, and 0.2% sodium azide, during 3 periods of storage: 7 days, 30 days and 6 months, resulting in 9 groups (n = 10). The roots were cut off and the buccal surface was ground with #600-grit silicon carbide paper. The surface was conditioned with 37% phosphoric acid for 15 s and a composite resin restoration (TPH Spectrum) was fixed using a one-bottle adhesive system (Adper Single Bond) and a dual-cured resinous cement (Rely X ARC) under a load of 500 g for 5 minutes. The samples were serially cut perpendicular to the bonded interface to obtain slices of 1.2 mm in thickness. Each slab was trimmed with a cylindrical diamond bur resulting in an hourglass shape with a cross-sectional area of approximately 1 mm². The microtensile bond strength (μTBS) testing was performed in a testing machine (EMIC 2000 DL) at a 0.5 mm/minute crosshead-speed until failure. After fracture, the specimens were examined under SEM to analyze the mode of fracture. μTBS Means were expressed in MPa and the data were analyzed by two-way ANOVA (3X3) and the Tukey test (α = 0.05). The storage times of 7 and 30 days produced no significant difference irrespective of the solution type. The formalin and thymol solutions, however, did have a negative influence on bond strength when the teeth were stored for 6 months.

Noncarious cervical lesions (NCCLs) are considered to be of multifactorial origin, normally associated with inadequate brushing. This study assessed the influence in vitro of simulated brushing on NCCL formation. Fifteen human premolars were submitted to brushing in the cementoenamel junction region, using hard-, medium- and soft-bristled brushes associated with a toothpaste of medium abrasiveness under a 200 g load, at a speed of 356 rpm for 100 minutes. The surface topography of the region was analyzed before and after brushing, by means of a laser interferometer, using "cut-off" values of 0.25 and considering roughness values in mm. The initial roughness (mm) results for dentin (D / bristle consistency: 1 - soft, 2 - medium and 3 - hard) were as follows: (D1) 1.25 ± 0.45; (D2) 1.12 ± 0.44; (D3) 1.05 ± 0.41. For enamel (E / bristle consistency: 1 - soft, 2 - medium and 3 - hard), the initial results were: (E1) 1.18 ± 0.35; (E2) 1.32 ± 0.25; (E3) 1.50 ± 0.38. After brushing, the following were the values for dentin: (D1) 2.32 ± 1.99; (D2) 3.30 ± 0.96; (D3) Over 500. For enamel, the values after brushing were: (E1) 1.37 ± 0.31; (E2) 2.15 ± 0.90; (E3) 1.22 ± 0.47. Based on the results of the ANOVA and Tukey statistical analyses (a = .05) it was concluded that soft, medium and hard brushes are not capable of abrading enamel, whereas dentin showed changes in surface roughness by the action of medium- and hard-bristled brushes.