ABSTRACT Aberrant expression of long non-coding RNAs (lncRNAs) has been detected in several types of cancer, including acute lymphoblastic leukemia (ALL), but lncRNA mapped on transcribed ultraconserved regions (T-UCRs) are little explored. The T-UCRs uc.112, uc.122, uc.160 and uc.262 were evaluated by quantitative real-time PCR in bone marrow samples from children with T-ALL (n = 32) and common-ALL/pre-B ALL (n = 30). In pediatric ALL, higher expression levels of uc.112 were found in patients with T-ALL, compared to patients with B-ALL. T-cells did not differ significantly from B-cells regarding uc.112 expression in non-tumor precursors from public data. Additionally, among B-ALL patients, uc.112 was also found to be increased in patients with hyperdiploidy, compared to other karyotype results. The uc.122, uc.160, and uc.262 were not associated with biological or clinical features. These findings suggest a potential role of uc.112 in pediatric ALL and emphasize the need for further investigation of T-UCR in pediatric ALL.

BACKGROUND During routine Coronavirus disease 2019 (COVID-19) diagnosis, an unusually high viral load was detected by reverse transcription real-time polymerase chain reaction (RT-qPCR) in a nasopharyngeal swab sample collected from a patient with respiratory and neurological symptoms who rapidly succumbed to the disease. Therefore we sought to characterise the infection. OBJECTIVES We aimed to determine and characterise the etiological agent responsible for the poor outcome. METHODS Classical virological methods, such as plaque assay and plaque reduction neutralisation test combined with amplicon-based sequencing, as well as a viral metagenomic approach, were performed to characterise the etiological agents of the infection. FINDINGS Plaque assay revealed two distinct plaque phenotypes, suggesting either the presence of two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains or a productive coinfection of two different species of virus. Amplicon-based sequencing did not support the presence of any SARS-CoV-2 genetic variants that would explain the high viral load and suggested the presence of a single SARS-CoV-2 strain. Nonetheless, the viral metagenomic analysis revealed that Coronaviridae and Herpesviridae were the predominant virus families within the sample. This finding was confirmed by a plaque reduction neutralisation test and PCR. MAIN CONCLUSIONS We characterised a productive coinfection of SARS-CoV-2 and Herpes simplex virus 1 (HSV-1) in a patient with severe symptoms that succumbed to the disease. Although we cannot establish the causal relationship between the coinfection and the severity of the clinical case, this work serves as a warning for future studies focused on the interplay between SARS-CoV-2 and HSV-1 coinfection and COVID-19 severity.

Resumo: Introdução: O objetivo deste estudo foi avaliar a progressão da competência, a curva de aprendizagem e o grau de satisfação com o modelo de treinamento de estudantes de Medicina submetidos a capacitação para realização de pontos laparoscópicos em simulador. Método: Trata-se de estudo prospectivo, longitudinal, intervencionista, realizado de abril de 2016 a julho de 2017, com participação de 52 estudantes de Medicina do Centro Universitário Christus, de Fortaleza, Brasil, do primeiro ao terceiro ano, submetidos a treinamento teórico prático, sistematizado, metodizado, com progressão de habilidades para realização de pontos laparoscópicos em ambiente de simulação, em quatro etapas com duração total de 16 horas. Foi estabelecida a tarefa de realizar pontos laparoscópicos, com cinco seminós, nas etapas inicial e final, em molde de sutura, em simulador de cavidade abdominal, em 18 minutos. Os desfechos principais foram o tempo e a qualidade de execução. Os estudantes foram avaliados antes da primeira e depois de todas as etapas do treinamento quanto à quantidade e qualidade dos pontos ou quanto ao conteúdo da estação e satisfação com o modelo de treinamento. Realizaram-se os testes ANOVA e T de Student para as amostras independentes e o teste do qui-quadrado para as categóricas. Para variáveis com medidas seriadas, utilizou-se o modelo linear geral. Utilizaram-se modelos binomiais univariados nas variáveis de avaliação do modelo de treinamento. Foram considerados significativos os valores de p menores que 0,05. Resultados: Analisaram-se os valores das medianas, entre a primeira e última etapas do treinamento, do número de seminós (0,0 e 15,0) e pontos laparoscópicos (0,0 e 3,0), da adequação dos tamanhos dos cotos dos fios (0,0 e 11,0), do número de seminós iniciais ajustados (0,0 e 3,0) e sequenciais ajustados (0,0 e 24,0). Houve significância estatística em todos os parâmetros avaliados (p < 0,001). A curva de aprendizagem mostrou que 99,1% dos estudantes atingiram a competência. O grau de avaliação satisfatória do modelo de treinamento foi considerado bom ou ótimo em 97% ou mais, com relevância estatística em oito das dez afirmações avaliadas. Conclusões: O estudo revelou que os alunos apresentaram progressão na competência e evolução na curva de aprendizagem. O grau de satisfação dos estudantes em relação às etapas do modelo de treinamento foi muito significativo.

Abstract: Introduction: To evaluate the progression of competence, learning curve and degree of satisfaction with the training model of medical students undergoing training to perform laparoscopic knots in a simulator. Methods: This was a prospective, longitudinal, interventional study, carried out from April 2016 to July 2017, with the participation of 52 students from the Centro Universitário Christus, Fortaleza, Brazil, from the first to the third year of medical school, undergoing theoretical practical, systematic, and methodized training, with progression of skills to perform laparoscopic knots in a simulation environment in four stages, with a total duration of 16 hours. It was established the task of performing laparoscopic stitches, with five simple knots, in the beginning and in the end, in a suture mold, in an abdominal cavity simulator, in 18 minutes. The main outcomes were time and quality of performance. The students were evaluated before the first and after all stages of the training regarding the quantity and quality of the knots or the subject of the stage and satisfaction with the training model. ANOVA and Student’s t tests were performed for the independent samples and the chi-square test for the categorical variables. For variables with serial measurements, general linear models were used. Univariate binomial models were used in the evaluation variables of the training model. P values <0.05 were considered significant. Results: The values of the medians were analyzed between the first and last stages of the training; of the number of simple knots (0.0 and 15.0) and laparoscopic knots (0.0 and 3.0), the adequacy of the sizes of the suture tail ends (0.0 and 11.0), the number of adjusted initial simple knots ( 0.0 and 3.0) and adjusted sequential ones (0.0 and 24.0). There was statistical significance in all evaluated parameters (p< 0.001). The learning curve showed that 99.1% of the students attained competence. The degree of satisfactory evaluation of the training model was considered good or great in 97% or more, with statistical relevance in 8 of the 10 evaluated statements. Conclusions: The study demonstrated that the students showed competence progression and learning curve evolution. The degree of student satisfaction in relation to the training model stages was very significant.

Abstract Purpose: To develop and test a model of teaching by means of an abdominal cavity simulator. Methods: This study had two stages: development of a teaching model and an experimental prospective study that aimed to evaluate the residents’ competence. The participants were divided into 3 groups: first-year resident, second-year resident, and senior surgeon. The two groups of resident physicians received training in the simulator, under instructor supervision for skill acquisition, according to the model proposed in first stage. The surgeons did not receive this intervention. The correlations and associations were verified through simple and multiple linear regressions. The learning curves were analysed using Cox regression models. The impact of the epidemiological characteristics was tested. Results: All residents reached the maximum score at the end of 16 steps and were comparable to the experimental (p<0.001). Conclusion: Residents who underwent training using the methodology of the proposed teaching model, which is based on realistic simulation, acquired proficiency in the accomplishment of endosutures in up to 16 hours of training in the laboratory.

RESUMO A aquisição de habilidades psicomotoras em cirurgia é o componente central dos programas de residência médica em Cirurgia Geral e Especialidades. Tornar o aprendizado mais efetivo é ponto basilar dos processos educacionais. Esse artigo retrata os aspectos das taxonomias educacionais e teorias de aprendizagem que podem ser envolvidas no treinamento da cirurgia. Entre as inúmeras taxonomias e teorias educacionais aplicáveis no aprendizado em cirurgia destacam-se: 1) Taxonomia de Dave- Hierarquização a ações que facilitam a aquisição de habilidades psicomotoras; 2) Teoria de Miller- Definição do passo a passo que facilita a aquisição das habilidades; 3) Teoria de Ericsson- Competência após a repetição da prática seguida de reforço sistemático; 4) Teoria de Vigotsky- Definição do papel do especialista no aprendizado; 5) Teoria de Boud, Schon e Ende- Importância da devolutiva (feedback) para alunos e professores. O conhecimento dessas ferramentas por professores e preceptores pode facilitar o aprendizado na cirurgia, em especial nas atividades mais complexas.

ABSTRACT The acquisition of psychomotor skills in surgery is the central component of medical residency programs in General Surgery and Specialties. Making learning more effective is a cornerstone of educational processes. This article portrays aspects of educational taxonomies and learning theories that may be involved in the training of surgery. Among the many taxonomies and educational theories applicable to learning in surgery, the following stand out: 1) Dave's taxonomy- Hierarchy to actions that facilitate the acquisition of psychomotor skills; 2) Miller’s theory- Step-by-step definition that facilitates acquisition; 3) Ericsson’s theory- Competence after repetition of the practice followed by systematic reinforcement; 4) Vigotsky's theory- Definition of the role of the specialist in learning; and 5) Theory of Boud, Schon and Ende- Importance of feedback for students and teachers. Knowledge of these tools by teachers and preceptors can facilitate learning in surgery, especially in more complex activities.

RESUMO: Este artigo apresenta as principais aplicações de veículos elétricos no meio rural, apontanto as tendências e mudanças futuras. O trabalho discute também as condições tecnológicas e dificuldades enfrentadas pelo setor industrial para a inserção do veículo elétrico no mercado. São apresentados os principais pesquisadores da área com propostas para superar os problemas da implementação dos tratores elétricos, tais como abastecimento e armazenamento de energia elétrica. As comparações técnicas e econômicas entre tratores convencionais, a combustão interna, e tratores elétricos são apresentadas e discutidas. A revisão mostra a existência de barreiras para a implantação dos veículos elétricos no meio rural, como a necessidade de evolução tecnológica das baterias, que apresentam alto custo e ainda são pesadas para este propósito, mas mostra também que já existem sistemas que podem minimizar a dependência de compubstíveis fóceis do setor e aumentar o uso de energia sustentável.

ABSTRACT: This paper presents the main applications of electric vehicles in rural areas, pointing out the trends and challenges for the future. Technological conditions and difficulties faced by the industry for a wide dissemination of this technology are discussed. The paper described the main researches with proposals to overcome the problems of implementing electric tractors, as supply and electricity storage. Technical and economic comparisons between conventional internal combustion tractors and electric tractors are also presented and discussed. The paper showed the existence of barriers to the implementation of electric vehicles in rural areas, as well as the need for batteries technological evolution, which have high costs and for that reason they are very heavy for these purposes, but there are already systems that can be applied to minimize dependence of fossil fuels in this sector and increase the use of sustainable energy.

RESUMO: A competitividade no mercado agrícola tem tornado intensa a busca pela redução de custo nos processos de produção, como é o caso da colheita do café ( Coffea arabica L.), que tem migrado da colheita manual para a semi mecanizada e mecanizada. Essa transição proporcionou o surgimento de novas tecnologias e diversos modelos de colhedoras, as quais permitem regulagens que influenciam diretamente na eficiência de derriça. Entretanto, entender o comportamento dinâmico dos componentes e sistemas das colhedoras é de extrema importância para o desenvolvimento de novos produtos e recomendações de regulagem para colheita plena ou seletiva. Nesse contexto, objetivou-se analisar a vibração das hastes derriçadoras de uma colhedora de café, em diferentes regulagens, a fim de entender seu comportamento dinâmico em termos de amplitude e frequência de vibração, e inferir sobre a recomendação de regulagens para a colheita mecânica do café. A metodologia utilizada, baseada na análise dos sinais de vibração das hastes derriçadoras, possibilitou a recomendação de regulagens para a colheita mecânica do café, em função das amplitudes de vibração das hastes derriçadoras. O estudo mostrou que as maiores amplitudes de vibração das hastes no sentido vertical ocorreram para as regulagens de 8 e 10Kgf no freio, na vibração de 950 ciclos min-1 do cilindro e com hastes de 570mm de comprimento. Os resultados permitem associar o comportamento de vibração das hastes derriçadoras com os objetivos da colheita, plena ou seletiva, apontando qual a regulagem específica a ser utilizada para cada finalidade.

ABSTRACT: Growers need to reduce costs of the coffee production to meet the demand and stay competitive in the market. Such a need has led to the application of mechanization in the coffee harvest ( Coffea Arabica L.), which is a costly process and requires high cost of manpower, and this last is increasingly scarce. The migration from manual harvest to semi-mechanized or super-mechanized made new technologies and diverse models of coffee harvesting machines emerge. These coffee harvesting machines have settings that directly influence the harvesting efficiency. To understand the dynamic behavior of coffee harvesting machines components and systems is very important to develop new products, setting and operation norms. In this context, this reseach aimed to analyze the vibration of the harvester rods of an automatic coffee harvesting, by considering different adjustments, in order to understand its dynamic behavior in terms of amplitude and frequency of vibration, and to infer about adjustment recommendations for the mechanical harvesting coffee. Results showed that the harvesting studied presented larger amplitudes of vibration of stems vertically when set for 8 and 10Kgf of curb, at vibration of 950 cycles min1 of cylinder and with the stems of 570mm of length; the highest efficiency occurs when the rods vibrate vertically, promoting greater contact between the rods and side branches and fruit of the coffee plant. Also, the achieved results can be very useful as a guide for adjusting coffee harvesting machines, for research anddevelopment of new technologies to the benefit of the grower.

INTRODUÇÃO: Medicamentos endodônticos apresentam componentes tóxicos que provocam algum grau de reação inflamatória.OBJETIVO: Este estudo avaliou o efeito da laserterapia na resposta inflamatória causada por medicações intracanais, em tecido subcutâneo de ratos, por meio da análise quantitativa de mastócitos.MATERIAL E MÉTODO: Tubos de polietileno contendo as medicações foram implantados no dorso de 60 ratos, distribuídos em seis grupos: HS (pasta de hidróxido de cálcio P.A.); HL (pasta de hidróxido de cálcio P.A. e laserterapia); HPS (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado); HPL (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado e laserterapia); IS (iodofórmio e soro fisiológico) e IL (iodofórmio, soro fisiológico e laserterapia). Os animais foram eutanasiados oito e quinze dias após a cirurgia, as peças cirúrgicas foram removidas, processadas para inclusão em parafina e os cortes histológicos corados em Azul de Toluidina 0.2%, para quantificação dos mastócitos. A análise de variância (ANOVA) e o teste de Tukey post hoc foram aplicados para determinar diferenças significativas entre os grupos quanto ao número de mastócitos (p<0.05).RESULTADO: Nos grupos HL, HPL e IL houve uma diminuição de mastócitos em ambos os períodos experimentais em relação aos grupos HS, HPS e IS, porém não se observou diferença estatística significativa entre o grupo HPS e o HPL aos oito dias.CONCLUSÃO: A laserterapia foi eficaz em modular a intensidade da resposta inflamatória induzida pelos medicamentos endodônticos a partir da redução significativa na quantidade de mastócitos.

INTRODUCTION: Endodontic medications contain toxic components that cause varying degrees of inflammation.OBJECTIVE: This study evaluated the effect of laser therapy on the inflammatory response induced by intracanal medications implanted into the subcutaneous tissue of rats using a quantitative analysis of mast cells.MATERIAL AND METHOD: Polyethylene tubes containing the medications were implanted in the dorsum of 60 rats divided into six groups, including HS (P.A. calcium hydroxide paste), HL (P.A. calcium hydroxide paste and laser therapy), HPS (P.A. calcium hydroxide paste with camphorated paramonochlorophenol), HPL (P.A. calcium hydroxide paste with camphorated paramonochlorophenol and laser therapy), IS (iodoform with saline) and IL (iodoform with saline and laser therapy). The animals were euthanized eight or fifteen days after surgery, and samples were removed and embedded in paraffin. Histological sections were stained with 0.2% toluidine blue for the quantification of mast cells. Analysis of variance (ANOVA) and Tukey's post-hoc test were applied to determine significant differences in the number of mast cells between groups (p<0.05).RESULT: There was a decrease in mast cells for the HL, HPL and IL groups when compared with the HS, HPS and IS groups at both time points. There was no statistically significant difference between the HPS and HPL groups at the eight-day time point.CONCLUSION: Laser therapy was effective at modulating the inflammatory response induced by endodontic medications by significantly reducing the number of mast cells.

O trabalho foi realizado na Estação Experimental de Itambé, PE, para avaliar a distribuição da biomassa e nutrientes em povoamentos de Mimosa caesalpiniaefolia Benth. Foram estudados dois povoamentos, em solo e topografia similares com oito e 11 anos de idade, nos espaçamentos de 3,0 x 3,0 m e 4,5 x 4,5 m, respectivamente. A biomassa foi determinada para cada componente aéreo. As amostras de folhas, galhos, cascas e fuste foram coletadas e analisadas quimicamente. A biomassa total encontrada foi de 66,22 e 80,78 t/ha, nos povoamentos I e II, respectivamente. O material lenhoso (galhos e lenho) representou 96,52 e 97,98% da biomassa total, nos povoamentos I e II. A distribuição de biomassa nos povoamentos I e II foi, respectivamente; de galhos (44,99 e 53,40%), fuste (51,53 e 44,58%), casca (2,39 e 1,40%) e folhas (1,10 e 0,62%). A ordem de concentração de nutrientes nos dois povoamentos, em todos os componentes da parte aérea, de modo geral, obedeceu à seguinte ordem decrescente: nitrogênio > cálcio > potássio > magnésio > enxofre > fósforo.

This work was carried out at Itambé Experimental Station, in the State of Pernambuco, Brazil, to study biomass and nutrient distribution in stands of Mimosa caesalpiniaefolia Benth. Two stands with similar soil and topographical conditions were studied, involving plants eight and eleven years old, at 3.0 x 3.0 m and 4.5 x 4.5 m spacing, respectively. The biomass of each tree component was determined. Samples of leaves, branches, bark and stem were collected and chemically analyzed. Total biomass was 66.22 and 80.78 t/ha in stands I and II, respectively. Biomass distribution in stands I and II was: branches (44.99 and 53.40%), wood (51.53 and 44.58%), bark (2.39 and 1.40%) and leaves (1.10 and 0.62%). Nutrients of the components for both stands showed the following concentration trend: Nitrogen > Calcium > Potassium > Magnesium > Sulfur > Phosphorus.

Fanconi anaemia (FA) is a recessive autosomal disease determined by mutations in genes of at least eleven complementation groups, with distinct distributions in different populations. As far as we know, there are no reports regarding the molecular characterisation of the disease in unselected FA patients in Brazil. OBECTIVE: This study aimed to investigate the most prevalent mutations of FANCA and FANCC genes in Brazilian patients with FA. METHODS: Genomic DNA obtained from 22 racially and ethnically diverse unrelated FA patients (mean age ± SD: 14.0 ± 7.8 years; 10 male, 12 female; 14 white, 8 black) was analysed by polymerase chain reaction and restriction site assays for identification of FANCA (delta3788-3790) and FANCC (delta322G, IVS4+4A -> T, W22X, L496R, R548X, Q13X, R185X, and L554P) gene mutations. RESULTS: Mutations in FANCA and FANCC genes were identified in 6 (27.3%) and 14 (63.6%) out of 22 patients, respectively. The disease could not be attributed to the tested mutations in the two remaining patients enrolled in the study (9.1%). The registry of the two most prevalent gene abnormalities (delta3788-3790 and IVS4 + 4 -> T) revealed that they were present in 18.2% and 15.9% of the FA alleles, respectively. Additional FANCC gene mutations were found in the study, with the following prevalence: delta322G (11.4%), W22X (9.1%), Q13X (2.3%), L554P (2.3%), and R548X (2.3%) of total FA alleles. CONCLUSION: These results suggest that mutations of FANCA and FANCC genes are the most prevalent mutations among FA patients in Brazil.

O trabalho foi conduzido em casa de vegetação da UFRPE, no período de outubro de 2000 a maio de 2001, com o objetivo de avaliar o efeito da adubação fosfatada e regimes de corte na composição química e digestibilidade in vitro do capim-de-raiz (Chloris orthonoton Doell). Os tratamentos foram obtidos por meio de arranjo fatorial 3 x 2 x 2 três doses de adubo fosfatado (0, 100 e 200 kg ha-1 de P2O5), duas frequências de corte (30 e 40 dias) e duas intensidades de corte (5 e 15 cm) em um delineamento inteiramente casualizado, com quatro repetições. Houve efeito significativo para os teores de matéria seca e de proteína bruta (PB), sendo que as plantas não adubadas com fósforo apresentaram as mais elevadas concentrações de PB (102,3 g kg-1). Os maiores teores de FDN foram obtidos nas plantas colhidas a 15 cm (750,3 g kg-1), enquanto os teores de FDA, nas plantas colhidas aos 30 dias de idade (389,8 g kg-1). O teor de fósforo na parte aérea das plantas foi influenciado pelas doses do nutriente aplicado, frequências e intensidades de corte. As plantas adubadas com fósforo apresentaram maior digestibilidade in vitro da matéria seca.

The research was carried out in a greenhouse, at the UFRPE, from October 2000 to May, 2001. The objective was to evaluate the effect of phosphatic fertilization and harvest systems on the chemical composition and in vitro digestibility of the "capim-de-raiz" (Chloris orthonoton Doell). The treatments was obted in a factorial arrangement 3 x 2 x 2, with three rates of phosphorus fertilizer (0, 100 and 200 kg ha-1 of P2O5), two cut frequencies (30 and 40 days) and two cut intensities (5 and 15 cm) distributed in the design was a completely randomized with four replications. Plants without phosphorus fertilizer presented the highest CP concentration (102.3 g kg-1). The highest NDF values were obtained in the plants cut at 15 cm (750.3 g kg-1). The highest ADF concentration values were found in the plants cut at 30 days of regrowth (389.8 g kg-1). The phosphorus concentration in the plant tops was influenced by the phosphatic fertilization rates, cut frequencies and cut intensities. Plants without P fertilizer presented the highest in vitro dry matter digestibility.

O trabalho foi conduzido em casa de vegetação do Departamento de Zootecnia/UFRPE, no período de outubro de 2000 a maio de 2001, objetivando avaliar o efeito da adubação fosfatada e de diferentes regimes de corte sobre a produtividade e perfilhamento do capim-de-raiz (Chloris orthonoton, Doell). O delineamento utilizado foi o inteiramente casualizado, distribuído em um arranjo fatorial 3 x 2 x 2, sendo três doses de adubação fosfatada (0, 100 e 200 kg/ha de P2O5), duas freqüências de corte (30 e 40 dias) e duas intensidades de corte (5 e 15 cm). A produção de MS foi influenciada pelas interações freqüência x intensidade de corte e adubação fosfatada x freqüência de corte. A melhor combinação entre freqüência e intensidade de corte foi de 40 dias e 5 cm, propiciando produção de 10,66 g/vaso de MS. A adubação fosfatada propiciou melhor resultado, quando a freqüência de corte foi de 40 dias (11,70 g/vaso de MS). O perfilhamento do capim-de-raiz foi influenciado pela adubação, freqüência e intensidade de corte.

The work was carried out in a greenhouse, at the Animal Science Department/UFRPE, from October 2000 to May 2001, to evaluate the effect of phosphorus fertilization and different cut systems on the productivity and tillering of the capim-de-raiz (Chloris orthonoton, Doell). The experimental design was completely randomized, distributed in a factorial arrangement 3 x 2 x 2, being three levels of application of phosphorus fertilizer (0, 100 and 200 kg/ha of P2O5), two cut frequencies (30 and 40 days) and two cut intensities (5 and 15 cm). Significant interaction between cut frequency and cut intensity as well as phosphorus fertilization and cut frequency for the DM yield was observed. The best combination between cut frequency and cut intensity was 40 days and 5 cm, respectively, with 10.66 g/pot of DM. The phosphorus fertilization showed higher efficacy when the cut frequency was 40 days (11.70 g/pot of DM). Phosphorus fertilization, cut frequency and cut intensity showed significant effect on the tillering process.