ABSTRACT Tamarind (Tamarindus indica L.) is a species with promising potential for commercial production; therefore, studies involving practices of conducting culture in the initial stage are essential. The present study aimed to evaluate different shading levels and substrate compositions in the seedling production of tamarind. The experimental design was completely randomized in a 4×4 factorial scheme, four levels of shading (0%, 18%, 30%, and 50%) and four substrates (pine bark-based substrate pure and mixed with 50% vermiculite, and sphagnum peat-based substrate pure and mixed with 50% vermiculite), with four replicates of four seedlings. Growth and biometric relationships were evaluated at 40 and 98 d after transplantation (DAT). The results reported that seedlings formed on the sphagnum peat-based substrate on pure or associated with vermiculite at 98 DAT had a height greater than 35 cm and a total 8.5 g DM. In contrast, seedlings produced in pine bark-based substrate did not reach 20 cm in height and had less than 3 g total DM. The condition of 30% and 50% shading promoted greater growth in height, reaching an average height greater than 40 cm, while in the full sun environment, seedlings showed an average of less than 35 cm; however, for the other variables, full sun environment did not differ from 30% shading, producing good quality seedlings. Thus, the cultivation of seedlings in an environment with 0% or 30% shading, associated with the sphagnum peat-based substrate pure or mixed with 50% vermiculite promoted the production of tamarind seedlings with vigorous growth. In the best environments, the best substrates increased, on average, 41.3% number of leaves, 80.5% shoot dry mass, 56.8% root dry mass, and 71.0% total dry mass. In the best substrates, the best environments increased, on average, 18.0% number of leaves, 20.7% shoot dry mass, 8.7% root dry mass, and 14.3% total dry mass.

ABSTRACT Introduction: The mastopexy with implants and L-shaped scarring aims to correct grades I to II breast ptosis using implants in retroglandular space and retromuscular dual plane space, at the same surgical time. The objective is to describe the experience with the L-augmentation mastopexy technique, analyzing complications and reoperations. Methods: Retrospective study of 123 patients with mild to moderate breast ptosis, operated using the L-augmentation mastopexy technique from January 2011 to November 2021. Results: The mean age of patients was 35.6 years. The average volume of implants used was 315ml (range 175 to 600ml). The placement of the prosthesis was in retroglandular (46.5%) and dual plane retromuscular space (53.5%). The average operative time was two hours and fifty-four minutes. The main complications presented were superficial dehiscence (7.3%), recurrence of ptosis (7.3%) and unsightly scars (5.7%). Surgical reviews took place in 13 patients (10.5%). Conclusion: Safe and effective mastopexy technique with implant in the treatment of mild to moderate breast ptosis, providing well designed breasts with reduced L-shaped scars.

RESUMO Introdução: A mastopexia com implantes e cicatriz em L visa corrigir ptoses mamárias graus I a II com utilização de implantes em espaço retroglandular e retromuscular dual plane, no mesmo tempo cirúrgico. O objetivo é descrever a experiência com a técnica de mastopexia de aumento em L, analisando complicações e reoperações. Método: Estudo retrospectivo de 123 pacientes com ptose mamária leve a moderada, operadas pela técnica de mastopexia de aumento em L no período de janeiro de 2011 a novembro de 2021. Resultados: A média de idade das pacientes foi de 35,6 anos. O volume médio de implantes utilizado foi de 315ml (variação de 175 a 600ml). O posicionamento da prótese foi em espaço retroglandular (46,5%) e retromuscular dual plane (53,5%). O tempo médio operatório foi de duas horas e cinquenta e quatro minutos. As principais complicações apresentadas foram deiscências superficiais (7,3%), recorrência da ptose (7,3%) e cicatrizes inestéticas (5,7%). Revisões cirúrgicas aconteceram em 13 pacientes (10,5%). Conclusão: Técnica de mastopexia com implante segura e eficaz no tratamento da ptose mamária leve a moderada, proporcionando mamas bem projetadas com cicatrizes reduzidas em L.

Abstract Although maize is one of the main crops in the Northeast region, yield is still considered low when compared to other regions. One of the main solutions to increasing yield is the selection of cultivars adapted to the conditions of the Northeast region. Thus, the present study aims to use the Bayesian segmented regression model to evaluate the adaptability and stability of maize. The experiment was set up in a randomized block design with two repetitions, where 25 maize hybrids were evaluated in different states. Initially, the analysis of variance was performed. Then, the Bayesian approach of the segmented regression method was used to select the hybrids regarding adaptability and stability. There was a difference between the genotypes indicated using the a priori distribution and those indicated by the minimally informative a priori distribution. Hybrids 20A55HX, 2B433HX, 2B512HX, and P2830H were considered ideal for the Northeast region.

RESUMO O artigo tem como objetivo discutir a contribuição de Beti Rabetti (Maria de Lourdes Rabetti) sobre as relações entre teatro e culturas populares. É apresentado um panorama sucinto da trajetória da pesquisadora e das discussões empreendidas sobre o tema, tanto a partir de suas pesquisas e publicações como a partir de suas orientações, enfatizando-se sua discussão sobre a importância dos modos de produção teatral. Discute-se a noção de cultura popular segundo autores como Bakhtin, Burke, Canclini e José Jorge de Carvalho. Também são abordados aspectos do teatro cômico popular, do teatro de revista no Brasil e de manifestações de culturas tradicionais em sua relação com o teatro de Ariano Suassuna. Maria populares tema orientações enfatizandose enfatizando se teatral Discutese Discute Bakhtin Burke Carvalho Suassuna

ABSTRACT This article aims to discuss the contribution of Beti Rabetti (Maria de Lourdes Rabetti) to discussions on the relation between theater and popular cultures. A brief overview of the researcher’s career and discussions on the subject are presented, based on her research work and publications, and on her work as an advisor, focusing on her discussion on the importance of the modes of theatrical production. Popular culture is discussed from authors such as Bakhtin, Burke, Canclini and José Jorge de Carvalho. Aspects of popular comic theater, teatro de revista in Brazil and manifestations of traditional cultures in their relations with the theater of Ariano Suassuna are also discussed. Maria researchers researcher s presented publications advisor production Bakhtin Burke Carvalho

RÉSUMÉ L’article vise à discuter la contribution de Beti Rabetti (Maria de Lourdes Rabetti) sur la relation entre le théâtre et les cultures populaires. Un aperçu succinct de la trajectoire de la chercheuse et des discussions engagées sur le sujet est présenté, tant à partir de ses recherches et publications que de ses directions, soulignant sa discussion sur l’importance des modes de production théâtrale. La notion de culture populaire est discutée à partir d’auteurs tels que Bakhtin, Burke, Canclini et José Jorge de Carvalho. Des aspects du théâtre comique populaire, du théâtre de revue au Brésil et des manifestations des cultures traditionnelles dans leur relation avec le théâtre d’Ariano Suassuna sont également abordées. Larticle L article Maria populaires présenté directions limportance l importance théâtrale dauteurs d auteurs Bakhtin Burke Carvalho dAriano Ariano abordées

Abstract BRS A705 is an early-maturing cultivar developed by Embrapa, recommended for irrigated cultivation. The plants have high tillering and moderate resistance to the main diseases. This cultivar has short stature, which confers a greater tolerance to lodging. It presents high yield and long and fine grains of excellent quality. A A70 earlymaturing early maturing Embrapa cultivation diseases stature lodging quality A7

Para que as ações de promoção da saúde possam impulsionar mudanças na saúde dos usuários, faz-se necessário o desenvolvimento de competências que auxiliem na sua efetivação. O estudo avaliou os limites e as potencialidades para a incorporação dos domínios Diagnóstico; Planejamento; Implementação; e Avaliação e Pesquisa do Developing Competenciesand Professional Standards for Health Promotion Capacity Building in Europe (CompHP) na realidade de uma residência multiprofissional em Saúde da Família e Comunidade. Pesquisa qualitativa realizada com residentes, tutores e preceptores. Foi utilizada a Hermenêutica de Profundidade, à luz da Análise de Discurso. O desenvolvimento de competências nos domínios do Diagnóstico; Planejamento; Implementação; e Avaliação e Pesquisa foram estratégicos para a formação dos residentes. Elas apresentaram potencial para a implementação e qualificação das ações de promoção da saúde no âmbito da Atenção Primária em Saúde. Por outro lado, lacunas durante o processo formativo dos residentes não possibilitaram uma maior clareza para a aquisição dessas competências.

For health promotion actions to drive change, health professionals need to develop relevant competencies. This study assessed the limitations and potential for incorporating the domains Diagnosis, Planning, Implementation, Evaluation and Research of the Developing Competencies and Professional Standards for Health Promotion Capacity Building in Europe (CompHP) into the reality of a multiprofessional residency program in family and community health. We conducted a qualitative study with residents, tutors, and preceptors using the depth-hermeneutical approach and discourse analysis. The development of competencies in the diagnosis, planning, implementation, evaluation and research domains was key to resident training. The competencies showed potential for implementation and improvement in the quality of health promotion actions in primary care. However, gaps in the training process meant that the residency program did not provide greater clarity for the acquisition of these competencies.

Para que las acciones de Promoción de la Salud puedan dar impulso a cambios en la salud de los usuarios, es necesario el desarrollo de competencias que ayuden en su realización. l estudio evaluó los límites y las potencialidades para la incorporación de los dominios Diagnóstico, Planificación, Implementación, Evaluación e Investigación del Developing Competencies and Professional Standards for Health Promotion Capacity Building in Europe (CompHP) en la realidad de una Residencia Multiprofesional en Salud de la Familia y Comunidad. Investigación cualitativa realizada con residentes, tutores y preceptores. Se utilizó la Hermenéutica de Profundidad a la luz del Análisis de Discurso. El desarrollo de competencias en los dominios Diagnóstico, Planificación, Implementación, Evaluación e Investigación fueron estratégicos para la formación de los residentes. Ellas presentaron potencial para la implementación y calificación de las acciones de promoción de la salud en el ámbito de la Atención Primaria de la Salud. Por otro lado, las brechas durante el proceso formativo de los residentes imposibilitaron una mayor claridad en la adquisición de estas competencias.

Para que as ações de promoção da saúde possam impulsionar mudanças na saúde dos usuários, faz-se necessário o desenvolvimento de competências que auxiliem na sua efetivação. O estudo avaliou os limites e as potencialidades para a incorporação dos domínios Diagnóstico; Planejamento; Implementação; e Avaliação e Pesquisa do Developing Competenciesand Professional Standards for Health Promotion Capacity Building in Europe (CompHP) na realidade de uma residência multiprofissional em Saúde da Família e Comunidade. Pesquisa qualitativa realizada com residentes, tutores e preceptores. Foi utilizada a Hermenêutica de Profundidade, à luz da Análise de Discurso. O desenvolvimento de competências nos domínios do Diagnóstico; Planejamento; Implementação; e Avaliação e Pesquisa foram estratégicos para a formação dos residentes. Elas apresentaram potencial para a implementação e qualificação das ações de promoção da saúde no âmbito da Atenção Primária em Saúde. Por outro lado, lacunas durante o processo formativo dos residentes não possibilitaram uma maior clareza para a aquisição dessas competências.

Para que las acciones de Promoción de la Salud puedan dar impulso a cambios en la salud de los usuarios, es necesario el desarrollo de competencias que ayuden en su realización. l estudio evaluó los límites y las potencialidades para la incorporación de los dominios Diagnóstico, Planificación, Implementación, Evaluación e Investigación del Developing Competencies and Professional Standards for Health Promotion Capacity Building in Europe (CompHP) en la realidad de una Residencia Multiprofesional en Salud de la Familia y Comunidad. Investigación cualitativa realizada con residentes, tutores y preceptores. Se utilizó la Hermenéutica de Profundidad a la luz del Análisis de Discurso. El desarrollo de competencias en los dominios Diagnóstico, Planificación, Implementación, Evaluación e Investigación fueron estratégicos para la formación de los residentes. Ellas presentaron potencial para la implementación y calificación de las acciones de promoción de la salud en el ámbito de la Atención Primaria de la Salud. Por otro lado, las brechas durante el proceso formativo de los residentes imposibilitaron una mayor claridad en la adquisición de estas competencias.

For health promotion actions to drive change, health professionals need to develop relevant competencies. This study assessed the limitations and potential for incorporating the domains Diagnosis, Planning, Implementation, Evaluation and Research of the Developing Competencies and Professional Standards for Health Promotion Capacity Building in Europe (CompHP) into the reality of a multiprofessional residency program in family and community health. We conducted a qualitative study with residents, tutors, and preceptors using the depth-hermeneutical approach and discourse analysis. The development of competencies in the diagnosis, planning, implementation, evaluation and research domains was key to resident training. The competencies showed potential for implementation and improvement in the quality of health promotion actions in primary care. However, gaps in the training process meant that the residency program did not provide greater clarity for the acquisition of these competencies.

This study aimed to develop a reliable stability-indicating method (SIM) for amoxicillin 500 mg capsules (DP-drug product). A literature review addressing amoxicillin (AMX; DS-drug substance) forced degradation studies and the existing SIMs was conducted to verify the most significant outcomes. Subsequently, the forced degradation of DP and DS was carried out following the Brazilian Health Surveillance Agency (ANVISA) guidelines, including thermal degradation (dry and wet heat), acidic and alkaline hydrolyses, hydrogen peroxide oxidation, reaction with copper, and photodegradation. Both DS and DP were more susceptible to 0.015 M NaOH, resulting in approximately 50% degradation. AMX DS and DP were not significantly photodegraded, but some degradation products (PDegs) showed susceptibility to light exposure. Thermodegraded samples showing ≥ 10% degradation exhibited modified profiles in thermogravimetric (TG) and differential scanning calorimetric (DSC) analyses. The X-ray powder diffraction patterns (XRPD) of DS samples exposed to dry and wet heat displayed complete amorphization of AMX, attesting to the occurrence of physical degradation concomitantly with chemical degradation, which can alter the drug’s bioavailability. In contrast, the thermodegraded DP samples exhibited intact AMX crystals interspersed with the amorphous form, perhaps partly protected by the excipient. The validated SIM was able to detect and quantify about 80 PDegs.

ABSTRACT The aim of this study was to evaluate mango hybrids obtained by open pollination based on the physical and chemical traits of the fruit, and to analyze the genetic diversity among these hybrids to find plants that produce quality fruit both for direct consumption and for industrial processing. The hybrids under study were generated from seeds produced from crosses with unknown genotypes as a result of open pollination. The unknown genotypes were randomly scattered in areas dedicated to growing a Tommy Atkins variety, and plants were obtained from mango seeds generated through sexual propagation. To assess genetic diversity, cluster analysis was carried out, as well as principal component analysis. Two hybrids were most prominent in terms of fruit weight, fruit length, fruit diameter, pulp weight, and pulp yield, with mean values greater than 245 g for pulp weight and 70% for pulp yield. Regarding fiber content in the pulp, six hybrids had a score of "2", that is, moderately fibrous fruit. For acidity and soluble solids content, the hybrids exhibited variations from 0.19 to 1.06% of citric acid and from 13.1 to 20.6 ºBrix, respectively. For peel color, tones ranging from reddish orange to yellow and green were observed. As for pulp color, there was variation from orange to light yellow tones. There was variability among the mango hybrids regarding the fruit traits analyzed, and hybrids that produce fruit that combine traits of economic interest were identified.

RESUMO Objetivou-se avaliar híbridos de mangueira obtidos por polinização aberta, com base em caracteres físicos e químicos dos frutos, além de analisar a diversidade genética entre os referidos híbridos, a fim de encontrar plantas que produzam frutos com qualidade tanto para consumo direto quanto para processamento industrial. Os híbridos estudados foram gerados a partir do cruzamento entre genótipos desconhecidos, resultantes de polinização livre, casualmente dispersos em áreas cultivadas exclusivamente com a variedade Tommy Atkins, cujas plantas foram obtidas por meio de propagação sexuada. Para aferir a diversidade genética, procedeu-se à análise de agrupamento, além da análise de componentes principais. Dois híbridos se destacaram quanto à massa do fruto, ao comprimento do fruto, ao diâmetro do fruto, à massa da polpa e rendimento de polpa, com as médias superiores a 245 g para massa da polpa e a 70% para o rendimento de polpa. Com relação ao teor de fibras na polpa, seis híbridos apresentaram nota “2”, ou seja, frutos moderadamente fibrosos. Para a acidez e o teor de sólidos solúveis os híbridos apresentaram variações de 0,19 a 1,06% de ácido cítrico e de 13,1 a 20,6 ºBrix, respectivamente. Para a coloração da casca, observaram-se tons variando entre laranja avermelhado, amarelo e verde. Quanto à coloração da polpa, houve variação de tons alaranjados a amarelo-claro. Houve variabilidade entre os híbridos de mangueira quanto aos caracteres do fruto analisados e foram identificados híbridos que produzem frutos que reúnem caracteres de interesse econômico.

RESUMO A irrigação por pulsos pode proporcionar maiores rendimentos para a cultura do amendoim em comparação a irrigação contínua com a utilização de águas salobras, contudo, estudos para as condições do Nordeste brasileiro utilizando esta técnica de manejo da irrigação associada ao uso de águas salobras são incipientes. Diante disso, objetivou-se com o trabalho avaliar o efeito do uso de águas salobras e da irrigação por gotejamento pulsado e contínuo sobre a produção de grãos e características produtivas do amendoim (Arachis hypogaea L.). O delineamento experimental utilizado foi o de blocos casualizados, em esquema fatorial de 6 x 2, composto por seis condutividades elétricas da água de irrigação (CEa: 0,2; 1,6; 2,8; 4,0; 5,2 e 6,4 dS m-1), na qual foi adicionada NaCl a água de abastecimento (CEa: 0,2 dS m-1) até atingir as respectivas CEa, e duas formas de aplicação da irrigação por gotejamento (com pulsos e contínua), com quatro repetições. As variáveis analisadas foram: a massa fresca e seca da parte aérea de 10 grãos, das vagens, dos grãos e da produção, além do número de vagens, do número de grãos, da percentagem de vagens chochas (PerVC) e da percentagem de grãos perfeitos (PerGP). A salinidade influenciou negativamente todas as variáveis estudadas, com exceção do PerVC e da PerGP. As menores percentagens de vagens chochas foram obtidas com a irrigação pulsada que por sua vez demonstrou a maior produção de vagens e de grãos independentemente dos níveis de condutividade elétrica da água avaliados. Nas condições desta pesquisa, é viável produzir amendoim utilizando águas salobras com condutividade elétrica de até 2,98 dS m-1.

ABSTRACT Pulse irrigation may be more advantageous for peanut crops than continuous irrigation, when using brackish water. However, studies on pulse irrigation with brackish water considering the environmental conditions of the Northeast region of Brazil using are incipient. Therefore, the objective of this study was to evaluate the effect of using brackish water for pulse and continuous dripping irrigations on the grain yield and production characteristics of peanut (Arachis hypogaea L.) crops. A completely randomized block design with four repetitions was used, in a 6*2 factorial arrangement consisted of six electrical conductivities of the irrigation water (EC; 0.2, 1.6, 2.8, 4.0, 5.2, and 6.4 dS m-1) set by adding NaCl to the irrigation water, which originally had 0.2 dS m-1, until reaching the respective EC; and two irrigation methods (pulse and continuous). The evaluated variables were fresh and dry weights of shoot, 10 grains, pods, grains, and production; number of pods and grains; and percentages of empty pods (EP%) and perfect grains (PG%). The addition of salt to the irrigation water negatively affected all variables studied, except for EP% and PG%. The lowest EP% were found for the pulse irrigation, which increased the pod and grain yields, regardless of the EC of the irrigation water. Under the conditions of the present study, the use of brackish waters with electrical conductivities of up to 2.98 dS m-1 is viable for peanut production.

ABSTRACT Purpose The aim of this work was to analyze the effect of fibrin biopolymer sealant (FS) associated or not to aquatic exercise (AE) on the calcaneal tendon repair. Methods Forty-four female Wistar rats were randomly divided into four experimental groups: Lesion control (L), Lesion and FS (LS), Lesion and AE (LE) and Lesion and FS associated to AE (LSE). The edema volume (EV), collagen ratio, and histopathological analysis were evaluated after 7, 14, and 21 days of partial tendon transection. Results The EV was statistically reduced for all treatment groups after 7 and 21 days when compared to L group. The LS and LSE had the highest EV reduction after 21 days of treatment. The FS group didn’t induce tissue necrosis or infections on the histopathological analysis. It was observed tenocytes proliferation, granulation tissue and collagen formation in the tendon partial transection area in the FS group. The LSE demonstrated higher amount of granulation tissue and increased the collagen deposition at the injury site. Conclusions Our data suggests that the therapeutic potential of the association of heterologous fibrin biopolymer sealant with aquatic exercise program should be further explored as it may stimulate the regeneration phase and optimize calcaneal tendon recovery.

ABSTRACT Lutzomyia longipalpis is the natural vector of Leishmania (Leishmania) infantum, but it is also permissive for several Leishmania species that are related to cutaneous leishmaniasis (CL). Maranhao State (Northeast of Brazil) is endemic for CL and has the highest number of cases of diffuse cutaneous leishmaniasis (DCL) in the country. It is a rare disease associated with a defective immune response mainly caused by L. (L.) amazonensis. Additionally, the number of immunosuppressed patients infected with the etiologic agents of CL has increased, including regions in which the main vectors of CL are rare. Therefore, we investigated whether Lu. longipalpis is able to transmit L. (L.) amazonensis to uninfected and immunosuppressed mice, resulting in CL. For that, 291 sand flies took an initial blood meal in mice infected with L. (L.) amazonensis. Of these, 17 underwent a second feeding on uninfected and immunosuppressed mice (of which 58.8% were also positive for Leishmania according to data on the dissection of the intestine). After 27 days of infection, these mice exhibited leishmaniotic lesions. The occurrence of parasites on the animal’s skin was confirmed by limiting dilution and immunohistopathological analyses. Parasite DNA was also detected in paw lesions and inguinal lymph nodes. DNA sequencing confirmed the Leishmania species in insects and mice. The results confirmed the ability of Lu. longipalpis to become infected and experimentally transmit L. (L.) amazonensis to immunosuppressed rodents, resulting in leishmaniotic lesions. Our data open perspectives for the potential role of Lu. longipalpis in the epidemiology of urban cutaneous leishmaniasis, especially in immunosuppressed patients.

ABSTRACT Chloroquine is the first-line therapy against the asexual stages of Plasmodium vivax . There is a high variation of chloroquine plasma levels after therapeutic doses, which can lead to inadequate exposure to the drug. The gender influence was low regarding the disposition of the drug, which is relevant as there are significant physiological variations between male and female patients. The objective of the study was to investigate whether gender modifies the pharmacokinetics parameters of chloroquine in patients with malaria vivax. A prospective study was performed in male and female adult patients using chloroquine (total dose of 25 mg/kg for three days) combined with primaquine. Serial blood samples were collected at admission and up to 672 h post-administration of the drugs. Chloroquine was measured in plasma samples by high-performance liquid chromatography with fluorescence detection. A non-compartmental analysis was used for modeling the data. A total of 26 male and 25 female patients were enrolled in the study. The pharmacokinetic parameters of chloroquine were similar between male and female patients: a half-life of 9.5 days and 10.2 days, maximum concentration (Cmax) of 1295 ng/ml and 1220 ng/ml, area-under-the-curve (AUC 0–28) of 241 µg/mL h and 237 µg/mL h, observed clearance (CL/f) of 5.8 and 5.5 L/h and the volume of distribution (V/f) of 1869 L and 1936 L. The study results suggest that a similar dose regimen of chloroquine combined with primaquine provides a comparable pattern of exposure in male and female patients.

Resumen Antecedentes: El momento mas apropiado para la recolección y transferencia de embriones en equinos -que es fundamental para el éxito de esta biotecnología- continua siendo sujeto de estudio. Además, es escasa la información sobre esta tecnología en condiciones tropicales. Objetivo: Determinar el momento mas adecuado para la recolecta y transferencia de embriones en yeguas Mangalarga Marchador, en las condiciones del nordeste Brasileño. Métodos: Donadoras (n= 30) y receptoras (n= 76) en la fase de diestro se seleccionaron con base en los exámenes clínicos y ginecológicos. El estro de las yeguas donadoras y receptoras fue inducido con 5 mg de Dinoprost, vía intramuscular, intentando obtener un intervalo de ovulación de -1 a +3 entre la receptora y la donadora. La ovulación fue inducida con acetato de buserelina cuando el folículo mayor alcanzó 35 mm de diámetro. En ese momento, las yeguas fueron sometidas a inseminación artificial en intervalos de 48 horas hasta la ovulación. Los embriones fueron recolectados en los días 7, 8 y 9 después de la ovulación. Resultados: La recolecta de embriones en el día 8 fue más eficiente (p<0,05) que en el día 7, pero no fue más efectivo (p>0,05) que en el día 9, el cuál presentó la misma eficiencia (p>0,05) que en el día 7. De un total de 76 embriones transferidos a las receptoras, que se encontraban entre el día 4 y 9 después de la ovulación, no se registró influencia (p>0,05) del día de la transferencia en la tasa de preñez. Conclusiones: La recolecta embrionaria debe ser realizada el día 8 después de la ovulación, y la transferencia puede ser realizada en cualquier día de este intervalo (4 a 9) sin que se afecte la tasa de preñez.

Abstract Background: Proper timing for embryo collection and transfer in horses -which is critical for the success of this biotechnology- is still debated. Additionally, there is little information on this technology under tropical conditions. Objective: To determine the best day for collection and transfer of embryos in Mangalarga Marchador mares under Brazilian northeast’s conditions. Methods: Donors (n= 30) and recipients (n= 76) in diestrus phase were selected based on both clinical and gynecology examinations. Estrus was induced on both donor and recipient mares by intramuscular injection of 5 mg Dinoprost, aiming to obtain an ovulation interval of -1 to +3 between recipient and donor. Ovulation was induced with buserelin acetate when the largest follicle reached at least 35 mm in diameter. At this time, mares were subjected to artificial insemination at 48-hour intervals until ovulation. The embryos were collected on days 7, 8, and 9 after ovulation. Results: The embryo collection on day 8 was more efficient (p<0.05) than on day 7, but it was not more effective (p>0.05) than day 9, which presented the same efficiency (p>0.05) as day 7. From a total of 76 embryos transferred to the recipients, that were between days 4 and 9 after ovulation, there was no influence (p>0.05) of the day of transfer on pregnancy rate. Conclusions: The embryo collection must be performed on day 8 after ovulation, and transfer can be performed on any day of that interval (4-9) without affecting the pregnancy rate.

Resumo Antecedentes: A importância do momentoda colheita e da transferência do embrião equino para o sucesso dessa biotécnica em equino continua sem ser completamente entendida. Adicionalmente, existe pouca informação sobre essa tecnologia em condições tropicais. Objetivo: Determinar o melhor dia para colheita e para transferência de embriões em eguas manga larga marchador nas condições do nordeste brasileiro. Métodos: Doadoras (n = 30) e receptoras (n = 76) na fase de diestro foram selecionadas com base nos exames clínico e ginecológicos. O estro das éguas doadoras e receptoras foi induzido com 5 mg de Dinoprost administrado por via intramuscular, buscando obter um intervalo de ovulação de -1 a +3 entre a receptora e a doadora. A ovulação foi induzida com acetato de buserelina quando o foliculo maior alcançou o tamanho de 35 mm de diâmetro. Nesse momento, as éguas foram submetidas a inseminação artificial em intervalos de 48 horas até a ovulação. Os embriões foram colhidos nos dias 7, 8 e 9 depois da ovulação. Resultados: A colheita de embriões no dia 8 foi mais eficiente (p<0,05) do que no dia 7, porem não foi mais efetivo (p>0,05) do que o dia 9, o qual apresentou a mesma eficiência (p>0,05) que o dia 7. De um total de 76 embriões transferidos para as receptoras que se encontravam entre os dias 4 e 9 depois da ovulação, não se registrou influência (p>0,05) do dia da transferência sobre a taxa de prenhez. Conclusões: A colheita embrionária deve ser realizada no dia 8 depois da ovulação, e a transferência pode ser realizada em qualquer dia desse intervalo (4-9) sem que a taxa de prenhez seja afetada.