ABSTRACT We assessed the immature stages of Ceratopogonidae (Diptera) in artificial containers in an urban forest fragment in Manaus (Brazil), including their behavioral, biological and ecological information. In addition, we evaluated the effects of deforestation in an open and forested area on Ceratopogonidae communities. Immatures were sampled between August 2012 and July 2014 in artificial containers installed in both habitat types. We collected 685 immatures of seven morpho-species of Bezzia Kieffer, Culicoides Latreille, Dasyhelea Kieffer, Forcipomyia Meigen, and Palpomyia Meigen. In the open area, we recorded higher temperature and electrical conductivity values than in the forested area; however, these variables did not differ between seasons. Water volume was higher in open area and in rainy season, while pH was similar in both areas and seasons. Species richness was higher in forested area, but did not differ between seasons. We did not record differences in abundance between areas or seasons. Community composition differed between areas, but not between seasons. We provide the first records of Culicoides (Hoffmania) insignis Lutz and C. (Haematomyidium) quasiparaensis Clastrier in artificial containers from the state of Amazonas. Our results suggest that the preservation of forested areas in Amazonas is fundamental for the maintenance of the life cycle of some species of Ceratopogonidae.

Uma espécie nova da Região Neotropical, Stilobezzia (Stilobezzia) pseudopunctulata Cazorla & Ronderos, é descrita e ilustrada a partir de adultos macho e fêmea e exúvia pupal. Os adultos foram coletados nas províncias argentinas de Chaco, Formosa, Corrientes e Buenos Aires e em Santa Cruz, Bolívia. A pupa foi coletada em tapetes de macrófitas aquáticas flutuantes em um lago na Ilha de Marchantaria, nas proximidades de Manaus, Brasil. Esta espécie nova é comparada com a sua congênere semelhante Stilobezzia punctulata Lane, a partir da qual pode ser distinguida principalmente pela asa com apenas duas manchas escuras, parâmeros mais longos e em forma de gancho, órgão respiratório da pupa com 11-12 poros e processo apicolateral do segmento anal muito curto.

A new species from the Neotropical Region, Stilobezzia (Stilobezzia) pseudopunctulata Cazorla & Ronderos, is described and illustrated based on male and female adults and pupal exuviae. Adults were collected in the Argentinian provinces of Chaco, Formosa, Corrientes and Buenos Aires, and in Santa Cruz, Bolivia. The pupa was collected from mats of floating aquatic macrophytes in a lagoon in Ilha da Marchantería, in the vicinities of Manaus, Brazil. This new species is compared with its similar congener Stilobezzia punctulata Lane, from which it can be mainly distinguished by the wing with only two dark spots, the parameres longer and hook-shaped, the pupal respiratory organ with 11-12 pores and the very short apicolateral processes of the anal segment.

Pupa e adulto macho previamente desconhecidos de Neobezzia fittkaui Wirth & Ratanaworabhan (Diptera, Ceratopogonidae). A pupa de Neobezzia fittkaui Wirth & Ratanaworabhan, 1972 foi coletada em macrófitas aquáticas flutuantes (Salvinia auriculata Aubl., Salviniaceae) na Ilha da Marchantaria próximo a Manaus, Brasil e o adulto macho criado foi descrito, fotografado e ilustrado pela primeira vez. Esta é a primeira descrição para a pupa do gênero Neobezzia Wirth & Ratanaworabhan.

The previously unknown pupa and adult male of Neobezzia fittkaui Wirth & Ratanaworabhan (Diptera, Ceratopogonidae). The pupa of Neobezzia fittkaui Wirth & Ratanaworabhan, 1972, collected from a mat of floating fern (Salvinia auriculata Aubl., Salviniaceae) in Ilha da Marchantaria near Manaus, Brazil and the reared adult male are described, photographed and illustrated for the first time. This is the first detailed pupal description for the genus Neobezzia Wirth & Ratanaworabhan.

A larva de último estádio de Bromeliagrion rehni Garrison in De Marmels & Garrison, 2005 é descrita e ilustrada e informações bionômicas sobre seu hábitat são apresentadas. O estudo foi conduzido na Reserva Florestal Adolpho Ducke, localizada em Manaus, Amazonas, Brasil. Entre abril de 2003 e abril de 2005, foram realizadas 12 amostragens, seis no período chuvoso e seis no seco. Para cada amostragem, 12 bromélias de Guzmania brasiliensis Ule, 1907 (Bromeliaceae) foram analisadas, seis terrestres e seis epífitas, totalizando 144 amostras. Um total de 75 espécimes de B. rehni foi coletado. A relação entre a abundância das larvas e os parâmetros ambientais mensurados (volume (ml), pH, estação e estratificação) foi significativa (ANCOVA, F = 5,296, df = 130, p < 0,001). Larvas foram mais abundantes na estação chuvosa (p < 0,01) e o volume de água foi positivamente correlacionado com a abundância de B. rehni. A larva de B. rehni pode ser diferenciada da larva de B. fernandezianum, única espécie do gênero com larva descrita, pelo número de setas no premento e pelo padrão de coloração na região apical do fêmur. Pela primeira vez é reportada a associação dessa espécie com fitotelmata de Guzmania brasiliensis.

The last-stage larva of Bromeliagrion rehni Garrison in De Marmels & Garrison, 2005 is described and illustrated and bionomics and habitat information on this species are provided. The study was conducted in the Reserva Florestal Adolpho Ducke, located near Manaus, state of Amazonas, Brazil.Twelve samplings were done between April, 2003 and April, 2005: six in the rainy season and six in the dry season. In each sampling month, 12 bromeliads (Guzmania brasiliensis Ule, 1907, Bromeliaceae) were collected, six of which were terrestrial and six epiphytic, yielding144 samples. A total of 75 specimens of B. rehni were collected. The relationship between larval B. rehni abundance and the measured environmental parameters (volume (ml), pH, season and stratum) was significant (ANCOVA, F = 5.296, d.f. = 130, p < 0.001). Larvae were most abundant in the rainy season (p < 0.01) and water volume was positively related to the abundance of B. rehni. Larvae of B. rehni can be distinguished from those of B. fernandezianum (the only species in the genus with described larvae) by the number of setae in the prementum and by the color of the apical region of the femur. The association of this species with phytotelmata of G. brasiliensis is reported here for the first time.