Benznidazole (BNZ) has been employed in the treatment of Chagas disease; however, its low water solubility is a drawback, which can be overcome by incorporating BZN within nanostructured systems. The aim of this work was to validate a chromatographic method for determination of BZN incorporated in nanoemulsions (NE) and nanostructured lipid carriers (NLC). The method was validated according to the International Conference on Harmonization guidelines. No interferences from the excipients of both systems on the BZN peak were observed. Linearity presented a correlation coefficient higher than 0.99 in the range of 5.0-30.0 µg mL-1. Relative standard deviation values for intra- and inter-days precision were lower than 2% and mean recovery ranged from 93.6 to 105.7%. BZN-loaded nanocarriers exhibited physico-chemical characteristics acceptable for parenteral administration and the encapsulation efficiency was almost 100% for both systems. The method shows a good potential to be employed in the determination of BZN in lipid nanostructures.

Os autores relatam caso de uma paciente portadora de epilepsia que desenvolveu sintomas psicóticos e depressivos associados a essa patologia, discutindo-o em relação a seus aspectos psicopatológicos.

The authors report a case of a patient with epilepsy who developed psychotic and depressive symptons and discuss it in the light of psychopatological aspects.

Com o objetivo de verificar o efeito da inoculação na produtividade, na translocação de assimilados e de N, durante o desenvolvimento da planta de trigo (Triticum aestivum L. cv. BR-23), foi realizado este estudo em condições de campo, com duas estirpes de Azospirillum brasilense (245 e JA 04). A inoculação foi efetuada no momento da semeadura, com inoculante turfoso. As coletas dos materiais para as avaliações foram efetuadas na antese e na maturação. Não foi detectado efeito da inoculação na produção de grãos e na translocação de assimilados. Diferenças foram observadas em relação às doses de N (0, 15 e 60 kg ha-1). O teor de N no grão aumentou significativamente nos tratamentos com bactérias, sem adição de N. Esse aumento foi provavelmente decorrente da maior absorção de N após a antese, sem reflexo significativo no rendimento de grãos. Tal aumento no N absorvido após a antese foi superior em 66% ao tratamento sem inoculação, representando um incremento de cerca de 8,4 kg ha-1. Independentemente da inoculação e da dose de N estudadas, observou-se que aproximadamente 70% do N acumulado na antese foi translocado das partes vegetativas para o grão.

The productivity and the translocation of assimilates and nitrogen (N) were compared after inoculation of wheat (Triticum aestivum L., cv. BR-23) seeds with two strains of Azospirillum brasilense (strains 245 and JA 04) under field conditions. The inoculation of wheat seeds was done with a peat inoculant at sowing time. Plant material for evaluations were collected at anthesis and maturity. No differences in grain yield and in the translocation of assimilates resulting from inoculation were detected. Differences were observed in relation to N rates (0, 15, and 60 kg ha-1). N content in the grain increased significantly in the bacteria-inoculated treatments in which N was not added. This increase in N content in the grain with inoculation was probably due to higher N uptake after anthesis without any significant contribution on the grain yield. Such increment was of 8.4 kg ha-1 of N representing 66% more N than in no inoculated treatment. Regardless of the inoculation and the rate of N applied, it was observed that about 70% of the N accumulated at anthesis was translocated from vegetative parts to the grain.