Pesquisa | SciELO

Abstract Among the different handling techniques in aquaculture, the use of anesthetics has had a growing interest focused on guaranteeing animal welfare, and reducing possible stress situations during general handling. The aim of this study was to present the use of eugenol and lidocaine with non-invasive anesthesia procedures in Dormitator latifrons, in which the different stages of anesthesia (induction and recovery) were determined. One hundred and twenty healthy fish of average weight of 73.59 ± 13.53 g and standard length of 17 ± 1.36 cm were used. The experimental fish were subjected to fasting for 24 h prior to the tests. Five fish were subjected to eugenol (25, 50, 100, and 200 µL/L), and lidocaine (100, 200, 300, and 400 mg/L), in triplicate. The time to reach deep and recovery anesthesia were recorded and the data analyzed using ANOVA (α= 0.05). Organisms exposed to anesthetics evidenced early episodes of fast, short-distance swimming (initial hyperactivity) for short periods of time. Survival was 100% with both compounds and concentrations. Fish exposed to a eugenol concentration of 200 µL/L had longer anesthesia times and took longer time to recover (P<0.05). The most effective concentrations for eugenol and lidocaine were of 200 µL/L and 400 µL/L in juvenile fish, promoting rapid inductions, without compromising the conditions for the recovery of the fish. This work provides practical information for handling and transportation D. latiforns with the least possible stress and ensuring animal welfare.

Resumo Dentre as diferentes técnicas de manejo na aquicultura, o uso de anestésicos tem despertado interesse crescente voltado para a garantia do bem-estar animal, reduzindo possíveis situações de estresse durante o manejo geral. O objetivo deste estudo foi apresentar o uso de eugenol e lidocaína com procedimentos anestésicos não invasivos em Dormitator latifrons, nos quais foram determinadas as diferentes etapas da anestesia (indução e recuperação). Foram utilizados 120 peixes saudáveis, com peso médio de 73,59 ± 13,53 g e 17 ± 1,36 cm de comprimento, em jejum de 24 horas antes dos testes. Cinco peixes foram submetidos a eugenol (25, 50, 100 e 200 µL/L) e lidocaína (100, 200, 300 e 400 mg/L), em triplicata. O tempo para atingir a anestesia profunda e de recuperação foi registrado, os dados foram analisados com ANOVA (α= 0,05). Organismos expostos a anestésicos evidenciaram episódios precoces de nado rápido de curta distância (hiperatividade inicial) por curtos períodos de tempo. A sobrevivência atinge 100% com ambos compostos e concentrações. Peixes expostos a uma concentração de eugenol de 200 µL/L tiveram tempos de anestesia mais longos e demoraram mais para se recuperar (P<0,05). As concentrações mais efetivas para eugenol e lidocaína foram de 200 µL/L e 400 µL/L em peixes juvenis, promovendo induções rápidas, sem comprometer as condições de recuperação dos peixes. Este trabalho fornece informações práticas para o manejo e transporte de D. latifrons com o mínimo de estresse possível e garantindo o bem-estar animal.

https://doi.org/10.1590/1519-6984.271624

Validación de protocolo para transversalizar la perspectiva de género en las prácticas de diagnóstico de la carrera de psicología
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Mora-Guerrero, Gloria M.

; MacAdoo-Espinoza, Andrés S.

; Troncoso-Arcos, Jorge C.

; Riquelme-Bravo, Loreto A.

Formación universitaria

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Ago 2021, Volume 14 Nº 4 Páginas 15 - 24

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract: The present study describes the process of design and validation of a protocol aimed to guide gender-mainstreaming diagnosis within the context of practical training activities in psychology degrees. This is a qualitative study with an applied cutting-approach that involves a construction-based systematization process of curriculum documentation and professor and student interviews. This is validated by experts’ assessments and student-user feedback. The results show that the protocol developed here facilities student identification and awareness about their own biases and gender prejudices in psychological diagnoses. It is concluded that the protocol developed here improves the diagnostic competence of psychology students, improving their professional career profile.

Resumen: Este estudio describe el proceso de construcción y validación de un protocolo que tiene el propósito de orientar la transversalización de la perspectiva de género en el diagnóstico, en el contexto de actividades prácticas formativas de la carrera de psicología. Se trató de un estudio de tipo cualitativo con enfoque de corte aplicado que implicó un procedimiento de diseño de este instrumento a partir de la sistematización de documentos curriculares y entrevistas a estudiantes y docentes; así como su posterior examinación por medio de juicios de expertos y retroalimentaciones de estudiantes usuarios. Se encontró que el protocolo apoya al estudiante a identificar los sesgos que pueden tener sus propios prejuicios de género sobre el trabajo diagnóstico. Se concluye que este material contribuye al desarrollo de la competencia de diagnóstico en psicología y que puede ser un recurso valioso para el logro del perfil de egreso de los/as futuros/as profesionales.

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Clinical characteristics and evolution of hematological patients and COVID-19 in Argentina: a report from the Argentine Society of Hematology
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Basquiera, Ana L.

; García, Mercedes J

; Martinez Rolón, Juliana

; Olmedo, Julieta

; Laviano, Julia

; Burgos, Rubén

; Caeiro, Gastón

; Remaggi, Guillermina

; Raña, Pablo

; Paoletti, Mariano

; González, Carlos M.

; Fernández, Isolda

; Pavlovsky, Astrid

; Perusini, Maria A.

; Rodriguez, Andrea

; Guanchiale, Luciana

; Carvani, Analia

; Mandrile, Laura

; Figueroa, Flavia

; Vicente Reparaz, Ángeles

; Fragapane Mathus, Patricia N.

; Garate, Gonzalo

; Fauque, María E.

; Kantor, Gustavo

; Cruset, Soledad

; Gonzalez Lorch, Jacqueline S.

; Szelagowski, Milagros

; Giarini, María P.

; Oliveira, Natalia

; García, María C.

; Ventriglia, María V.

; Pereyra, Patricio H.

; Gutierrez, Daniel R.

; Kusminsky, Gustavo

; Troccoli, Josefina

; Freitas, María J.

; Cranco, Santiago

; Del V.Sanchez, Natalia

; Rey, Irene

; Funes, María E.

; Jarchum, Sol

; Freue, Julian

; Miroli, Augusto

; Guerrero, Osvaldo

; López Ares, Lisa

; Campestri, Reinaldo

; Bove, Victor

; Salinas, Graciela N.

; Cabrejo, María

; Milone, Jorge H.

; Zabaljauregui, Soledad

; Gotta, Daniel

; Dupont, Juan Carlos

; Stemmelin, German

Medicina (Buenos Aires)

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Ago 2021, Volume 81 Nº 4 Páginas 536 - 545

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Individuals with malignancies and COVID-19 have a lower survival compared with the general population. However, the information about the impact of COVID-19 on the whole hematological population is scarce. We aimed to describe the 30th day overall survival (OS) after COVID-19 infection in pa tients with a hematological disease in Argentina. A completely anonymous survey from the Argentine Society of Hematology was delivered to all the hematologists in Argentina; it started in April 2020. A cut-off to analyze the data was performed in December 2020 and, finally, 419 patients were reported and suitable for the analysis (average age: 58 years, 90% with malignant diseases). After the COVID-19 diagnosis, the 30-day OS for the whole population was 80.2%. From the entire group (419), 101 (24.1%) individuals required intensive care unit admission, where the 30-day OS was 46.6%. Among allogeneic stem cell transplant recipients, the 30-day OS was 70.3%. Factors associated with a low OS were two or more comorbidities, an active hematological disease and history of chemotherapy. In individuals with the three factors, the 30-day OS was 49.6% while the 30-day OS in those without those factors was 100%. Patients with hematological diseases have a higher mortality than the general population. This group represents a challenge and requires careful decision-making of the treatment in order not to compromise the chances of cure.

Resumen El presente estudio tuvo por objetivo primario conocer la mortalidad de pacientes con enfermedad hematológica que presentaron infección por COVID-19 en Argentina. Para ello se difundió una encuesta desde la Sociedad Argentina de Hematología (SAH) entre los hematológos para informar sobre los pacientes con enfermedades hematológicas y diagnóstico de infección por SARS- CoV-2, entre el 19/4/2020, y el 7/12/2020. Se incluyeron individuos de todas las edades con diagnóstico de enfermedad hematológica benigna o maligna e infección por SARS-CoV-2 confirmada por técnica de RT-PCR. Se analizaron 419 pacientes (mediana 58 años; 90% enfermedades malignas). La supervivencia al día 30 fue de 80.2%. La supervivencia fue menor en aquellos que requirieron internación (74.2%), cuidados intensivos (46.6%) y asistencia respiratoria mecánica (36.8%). Entre los trasplantados alogénicos la supervivencia fue 70.3%. Los factores vinculados a la supervivencia global fueron las comorbilidades, el estado de la enfermedad al momento de la infección y el antecedente de quimioterapia. Se pudo establecer un score en el que aquellos que tuvieron un puntaje de 4 alcanzaron una supervivencia del 49.6% al día 30, mientras que la de los pacientes con score 0 fue del 100% a 30 días. En comparación con la población general, los pacientes con enfermedades hematológicas presentan una mayor mortalidad vinculada al COVID-19, motivo por el cual es primordial definir pautas destinadas a disminuir la exposición de los mismos sin comprometer las posibilidades de beneficiarse del tratamiento de la enfermedad de base.

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Physical properties of soil in the early stage of an agroforestry system in the High Andean zone
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Muñoz-Rodríguez, M-Alonso

; Delgado-Vargas, Iván A.

; Guerrero J., Jennyfer Cristina

; Andrade B., Anyi Valeria

Revista de Ciencias Agrícolas

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Jun 2020, Volume 37 Nº 1 Páginas 70 - 79

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN En Nariño, Colombia, el cambio de uso del suelo ha generado problemas de erosión, compactación, pérdida de la capa fértil del suelo, entre otros, siendo necesario buscar alternativas que minimicen estos impactos. El estudio se realizó con el objetivo de evaluar algunas de las propiedades físicas del suelo en un sistema agroforestal con Morella pubescens (Humb. & Bonpl. ex Willd.) Wilbur, en asocio con Smallanthus sonchifolius Poepp.) H.Rob, en la Granja Experimental Botana, municipio de Pasto, donde se analizó la densidad aparente, densidad real, porosidad total, distribución de agregados y estabilidad estructural del suelo. Los datos se obtuvieron en dos fases; fase 1 (f1) y fase 2 (f2) antes y después de la implementación del sistema agroforestal, respectivamente. Se utilizó un diseño de bloques completos al azar con tres tratamientos y tres repeticiones. Los tratamientos correspondieron a densidades de siembra T1: S. sonchifolius sembrado a 1x1m, T2:0.8x0.8m y T3:0.5x0.5m. M. pubescens fue establecido a 4 x 4m entre plántulas y a 9m entre líneas de M. pubescens y el testigo. Se presentaron diferencias estadísticas significativas entre f1 (testigo) y f2 (tratamientos), para las propiedades estabilidad estructural y distribución de agregados. El suelo sufrió un cambio en la estabilidad estructural pasando de ligeramente estable (0.97 D.P.M) en f1 a moderadamente estable (2.53 D.P.M), en f2. La distribución de agregados pasó de un 60% en f1 a un 70.3% en la f2. El manejo de sistemas agroforestales puede contribuir a mediano y largo plazo en la mejora de la estructura del suelo mediante la incorporación del material vegetal del componente arbóreo, la aplicación de una labranza mínima y la adición de materia orgánica.

ABSTRACT In Nariño, Colombia, land use has generated some problems such as erosion, compaction, lack of fertility, among others, so it is necessary to search for alternatives to mitigate these impacts. The objective of this study was to evaluate some physical properties of the soil in an agroforest system with Morella pubescens (Humb. & Bonpl. ex Willd.) Wilbur with Smallanthus sonchifolius (Poepp.) H.Rob, in the experimental farm of Botana, in the city of Pasto, Nariño, where the bulk density, real density, total porosity, structural stability properties and distribution of aggregates were analyzed. The datas were obtained in two phases: f1 and f2 before and after agroforestry system implementation, respectively. A randomized complete block design with three treatments and three replications were used. The treatments corresponded to plant densities, T1: S sonchifoliuos, sowed to 1x1m, T2:0.8x0.8m y T3:0.5x0.5m, with three replications. M pusescens was stablished to 4x4m between plants and 9 m between rows and the control (f1). The statistics have differences between f1 (control) and f2 (treatments), to the structural stability properties and distribution of aggregates. The ground suffered a structural stability change to stable lightly (0.97 D.P.M) in f1 to a stable moderate (2.53 D.P.M), in f2. The distribution of aggregates passed to 60% in f1 to 70.3% in f2, with a media aggregation status. The different agroforest systems management could help, in the long term, by improving the structure of the ground through the vegetal material of arboreal component, the application of a minimum farming and the addition of organic matter.

https://doi.org/10.22267/rcia.203701.128 111 downloads

Effect of temperature gradients on the behaviour of jointed plain concrete pavements
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TRUJILLO, P. B.

; GUERRERO, M. A. S.

Revista IBRACON de Estruturas e Materiais

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Abr 2019, Volume 12 Nº 2 Páginas 398 - 407

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Em muitos países da América Latina os pavimentos de concreto com juntas (JPCP) são calculados usando os métodos da “American Association of State Highway and Transportation Officials (AASHTO) ou da “Portland Cement Association” (PCA), os quais não levam em consideração explicitamente as condições climáticas específicas de cada região onde um JPCP será construído. É muito conhecido o empenamento causado pelos gradientes térmicos que se apresentam através da espessura do pavimento, o qual pode ocasionar a perda de suporte nas extremidades ou no centro da placa dependendo do momento do dia. Esta situação, paralelamente com as cargas veiculares, produzem esforços de tensão que podem ser de suficiente importância para iniciar a fissuração e finalmente a falha completa por fadiga. Nesta investigação, o software de Elemento Finito (EF) EverFe2.25 foi usado para estudar o impacto que os gradientes termicos (calibrado para considerar os gradientes de humidade), em combinação com as cargas veiculares (tandem com duas rodas de 180 kN), tem sobre o comportamento de uma tipica seção de JPCP. Para o JPCP em estudo, o aumento aparente do esforço de tensão atribuído aos Gradientes Térmicos Equivalentes (ETGs) negativos locais (ΔT = -18.5°C) foi 5.5 vezes maior do que os esforços induzidos pelo eixo quando posicionado na borda da placa e até 8.8 vezes maior quando foi colocado o canto da placa. Apesar de que o incremento aparente não foi tão grande quando analisados os ETGs positivos (ΔT = +18.5°C), a amplitude absoluta do esforço calculado nas combinações com as cargas veiculares para estas situações são suficientemente grandes para que ocorra falha por fadiga. Portanto, os gradientes termicos locais tem que ser tomados em consideração para o calculo dos JPCP usando procedimentos de design mais adequados, como o procedimento Empírico-Mecanicista.

Abstract In many countries of Latin America, Jointed Plain Concrete Pavements (JPCP) are designed using the American Association of State Highway and Transportation Officials (AASHTO) or the Portland Cement Association (PCA) methods, none of which take explicitly into account the environmental conditions of the specific region where a JPCP will be built. It is well known that temperature gradients across the thickness of concrete slabs produce curling which can cause slabs to lose support at its corners and its center depending on the time of the day. This situation, in combination with vehicle loading, generates tensile stresses which can be significant enough to cause initial cracking and the subsequent failure of the pavement. In this research, the Finite Element (FE) package EverFE2.25 was used to study the impact of temperature gradients (calibrated to account for humidity gradients) in combination with a vehicle load (a dual wheel tandem axle of 180 kN) on the performance of a typical JPCP highway section. For the JPCP under study, the apparent increase of the tensile stresses attributable to negative local (ΔT = -18.5°C) Equivalent Temperature Gradients (ETGs) was as 5.5 times larger than the one induced by the axle when it was placed at the border of the slab and up to 8.8 times larger when it was placed at the corner of the slab. Although the apparent increase was not as high when the positive local ETGs (ΔT = +18.5°C) were used, the absolute magnitude of the stresses in combination with the vehicle load were still high enough for the pavement to fail under fatigue. Thus, local temperature gradients should be studied regionally and considered in the design of JPCP using more suitable design procedures such as the Mechanistic-Empirical design procedure.

https://doi.org/10.1590/s1983-41952019000200011 1391 downloads

Efecto inhibitorio de extractos de Vitex mollis Kunth contra bacterias y especies de Fusarium de importancia humana y agrícola
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Valencia-Botin, Alberto J.

; Gutiérrez-Lomelí, Melesio

; Morales-Del-Rio, Juan A.

; Guerrero-Medina, Pedro J.

; Robles-García, Miguel A.

; Ruiz-Cruz, Saúl

; Wong-Corral, Francisco J.

; Borboa-Flores, Jesús

; Rueda-Puente, Edgar O.

; Toro-Sánchez, Carmen L. Del-

Revista fitotecnia mexicana

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Dez 2018, Volume 41 Nº 4 Páginas 353 - 363

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Summary Currently, antibiotic resistance and indiscriminate use of chemical fungicides in agriculture need to be addressed. This research evaluated the inhibitory effect of aqueous, methanolic, acetonic and hexanic extracts from leaf and stem of Vitex mollis Kunth (Lamiaceae) against several bacteria (Escherichia coli, Micrococcus luteus, Salmonella enterica and Staphylococcus aureus) and species of Fusarium fungus (F. verticillioides, F. oxysporum, F. tapsinum and F. oxysporum f.sp. lycopersici), and determined their general chemical composition. The minimum inhibitory concentration (MIC) was determined by the micro-dilution technique for all extracts, except for the hexanic extract which did not show inhibition on the bacteria studied. S. enterica was the bacterium that showed the highest sensitivity to the methanolic extracts from stem (MIC = 28 µg mL-1), followed by M. luteus (MIC = 32 µg mL-1), S. aureus (MIC = 75 µg mL-1) and E. coli (MIC = 80 µg mL-1). The methanolic and aqueous extracts from the stem presented higher percentage of inhibition against different types of Fusarium evaluated by the agar dilution method. Extracts of V. mollis inhibited F. verticillioides between 62 and 91 % with 120 µg mL-1 of extract. The order of fungi species as inhibited by the extracts was F. verticillioides > F. oxysporum > F. tapsinum > F. oxysporum f.sp. lycopersici. The chemical composition of the species was determined by tests for phenols, tannins, flavonoids, triterpenes, alkaloids, coumarins and saponins. None of the extracts presented alkaloids and saponins. Phenols (37.1 mg EAG/g dry sample) and flavonoids (26.8 mg EQ/g dry sample) were the major compounds in the methanolic and aqueous extracts. In conclusion, small amounts of extracts are required to inhibit bacteria and Fusarium; hence, V. mollis can be considered a source of metabolites for this purpose and in agriculture as an alternative control within an integrated management of diseases.

Resumen Actualmente existe la necesidad de hacer frente al problema de la resistencia a los antibióticos y al uso indiscriminado de fungicidas químicos en la agricultura. El objetivo de este trabajo fue evaluar el efecto inhibitorio de extractos acuosos, metanólicos, acetónicos y hexánicos de hoja y tallo de Vitex mollis Kunth (Lamiaceae) contra diferentes bacterias (Escherichia coli, Micrococcus luteus, Salmonella enterica y Staphylococcus aureus) y especies del hongo Fusarium (F. verticillioides, F. oxysporum, F tapsinum y F. oxysporum f.sp. lycopersici) de importancia en la salud y en la agricultura, así como determinar su composición química general. Se determinaron las concentraciones inhibitorias mínimas (CIM) de todos los extractos por la técnica de microdilución, excepto del hexánico, que no presentó inhibición en las bacterias estudiadas. S. enterica fue la bacteria que mostró mayor sensibilidad al extracto metanólico de tallo (CIM = 28 µg mL-1), le siguieron M. luteus (CIM = 32 µg mL-1), S. aureus (CIM = 75 µg mL-1) y E. coli (CIM = 80 µg mL-1). Los extractos metanólicos y acuosos de tallo presentaron mayor porcentaje de inhibición contra los diferentes tipos de Fusarium evaluados por el método de dilución en agar. Los extractos de V. mollis inhibieron a F. verticillioides entre 62 y 91 % con 120 µg mL-1 de extracto. El orden de las especies de hongos inhibidas por los extractos fue: F. verticillioides > F. oxysporum > F. tapsinum > F. oxysporum f.sp. lycopersici. La composición química de las especies se determinó mediante pruebas para fenoles, taninos, flavonoides, triterpenos, alcaloides, cumarinas y saponinas. Ninguno de los extractos presentó alcaloides y saponinas. Los fenoles (37.1 mg EAG/g muestra seca) y flavonoides (26.8 mg EQ/g muestra seca) fueron los compuestos mayoritarios en los extractos metanólicos y acuosos. En conclusión, se requieren cantidades muy pequeñas de extracto para la inhibición de bacterias y de Fusarium; por lo tanto, V. mollis puede ser considerada una fuente de metabolitos para este fin y en la agricultura como control alternativo dentro de un manejo integrado de enfermedades.

https://doi.org/10.35196/rfm.2018.4.353-363 580 downloads

Design, Synthesis and Anticancer Biological Evaluation of Novel 1,4-Diaryl-1,2,3-triazole Retinoid Analogues of Tamibarotene (AM80)
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Aleixo, Mariana A. A.

; Garcia, Taís M.

; Carvalho, Diego B.

; Viana, Luiz H.

; Amaral, Marcos S.

; Kassab, Najla M.

; Cunha, Marilin C.

; Pereira, Indiara C.

; Guerrero Jr., Palimécio G.

; Perdomo, Renata T.

; Matos, Maria F. C.

; Baroni, Adriano C. M.

Journal of the Brazilian Chemical Society

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Jan 2018, Volume 29 Nº 1 Páginas 109 - 124

Resumo: En | Texto: En | PDF: En

We report herein the design and synthesis via click chemistry of twelve novel triazole retinoid analogues of tamibarotene (AM80) and the evaluation of their anticancer activities against six cancer cell lines: HL60, K562, 786, HT29, MCF7 and PC3. Among the synthesized compounds, two were more potent than tamibarotene against solid tumor cells, and one of them had similar potency to tamibarotene against HL60 cells. The bioisosteric exchange between the amide group and the 1,2,3-triazole core in the retinoid agent tamibarotene (AM80) reported in this work is a valid strategy for the generation of useful compounds against cancer.

https://doi.org/10.21577/0103-5053.20170119 1085 downloads

Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats
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Rizzi, E. S.

; Dourado, D. M.

; Matias, R.

; Muller, J. A. I.

; Guilhermino, J. F.

; Guerrero, A. T. G.

; Moreira, D. L.

; Silva, B. A. K.

; Barbosa-Ferreira, M.

Brazilian Journal of Biology

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Jul 2017, Volume 77 Nº 3 Páginas 480 - 489

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A cicatrização deficiente representa uma complicação grave em algumas patologias e o uso de extratos de plantas tem demonstrado melhora no processo de reparação tecidual. O objetivo deste estudo foi avaliar o potencial cicatrizante da pomada de Sebastiania hispida comparado ao Laser Indio Phosphide-Gallium-Aluminum (InGaAlP) em feridas cutâneas induzidas cirurgicamente em ratos e realizar analise fitoquímica. A análise fitoquímica foi via clássica e por CLAE. O experimento foi desenvolvido com 80 ratos (200-250 g), divididos aleatoriamente em quatro grupos (n = 5): G1 (salina); G2 (extrato bruto metanólico 2% + Carbopol Gel 98%); G3 (extrato bruto metanólico 2% + lanolina/vaselina) e G4 (laser). Após a tricotomia da região dorsal realizou uma excisão linear, medindo 2 cm × 1 cm (epiderme e derme), expondo a fáscia muscular. Em sequência os tratamentos e o monitoramento do processo de cicatrização das feridas cirúrgicas ocorreu nos tempos de: 3, 7, 14 e até 21 dias após a excisão. Os parâmetros histológicos avaliados foram: tipos de fibras colágenas, avaliação morfológica e neovascularização. Houve uma deposição significativa de fibras colágenas, evidenciado por um tecido epitelial mais bem organizado, queratinizado e mostrando uma maior proliferação de novos vasos sanguíneos na fase inflamatória do grupo tratado com o extrato e o laser. A eficiência do processo de cicatrização pode estar relacionada com a presença de compostos fenólicos e derivados detectados na análise qualitativa e quantitativa. A utilização do tratamento tópico com as duas formulações diferentes de S. hispida foram mais eficazes do que a aplicação do Laser (InGaAlP) no modelo utilizado.

Abstract Impaired wound healing represents a serious complication in some pathologies and the use of plant extracts has proved to improve tissue repair. The objectives of this study were to evaluate the healing potential of the ointment of Sebastiana hispida compared with Aluminum-Gallium Indium-Phosphide Laser (InGaAlP) in surgically induced wounds in rats and to perform the phytochemical analysis. The phytochemical analysis was performed in the classic way and also by HPLC. A controlled study was developed with 80 rats (200-250 g) in which a linear excision was performed in the dorsal region after shaving, measuring 2 cm × 1 cm (epidermis and dermis) exposing the muscle fascia. The rats were randomly divided into four groups of twenty animals each. The experimental groups (n = 5) were G1 (Saline); G2 (crude methanol plant extract 2% + Carbopol Gel 98%); G3 (crude methanol plant extract 2% + lanolin/vaseline) and G4 (laser). The incision healing processes were monitored during 3, 7, 14 and until 21 days after excision. The histologic parameters evaluated were Collagen fiber types, microscopic examination and neovascularization. There was a significant increase in the deposition of collagen fibers, as evidenced by a better organized epithelial tissue, keratinized and showing greater proliferation of new blood vessels in the inflammatory phase in the group treated with both the extract and laser. The results were correlated to the phenolic derivatives found after qualitative and quantitative analysis. These compounds were considered responsible for the healing process. The topical treatment with S. hispida leaves, in the two different formulations, was more effective than the application of the laser (Ingan ALP) 660 nm in the model used.

https://doi.org/10.1590/1519-6984.16115 1517 downloads

Stephanospora michoacanensis (Stephanosporaceae, Agaricales), a novel sequestrate truffle from North America
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Guevara-Guerrero, Gonzalo

; Báez-Alvarado, Ivone

; Gómez-Reyes, Víctor M.

; Castellano, Michael A.

Revista mexicana de micología

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2015, Volume 41 Páginas 73 - 77

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Stephanospora michoacanensis is presented as a new species from North America. This angiocarpic species is recognized by its yellow-cream peridial surface color and broadly ellipsoid to subglobose, spiny or crested, inamyloid spores with a distinct, complete or nearly complete corona at its base. Stephanospora michoacanensis is similar to S. caroticolor, but S. caroticolor has a bright yellow to reddish yellow peridial surface, and larger spores. Stephanospora michoacanensis also resembles S. chilensis, but S. chilensis has a brownish-ochraceous to reddish orange peridial surface, spiny spores that lack crest-like features. Illustrations of macro-and microscopic features are presented.

Se presenta Stephanospora michoacanensis como una nueva especie de América del Norte. Esta especie angiocárpica se reconoce por el color amarillo crema del peridio, las esporas subglobosas a globosas, con espinas o crestas inamiloides, y con una corona distintiva completa o casi completa en su base. Stephanospora michoacanensis es similar a S. caroticolor, pero esta última tiene un color amarillo brillante a amarillo rojizo en la superficie del peridio y basidiosporas más grandes. Stephanospora michoacanensis también se parece a S. chilensis, pero esta presenta el color del peridio pardo-ocráceo a anaranjado-rojizo, y esporas espinosas sin crestas. Se incluyen ilustraciones de los caracteres, tanto macro como microscópicos.

816 downloads

10.

Liberación de péptidos bioactivos por bacterias lácticas en leches fermentadas comerciales
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González-Olivares, L.G.

; Jiménez-Guzmán, J.

; Cruz-Guerrero, A.

; Rodríguez-Serrano, G.

; Gómez-Ruiz, L.

; García-Garibay, M.

Revista mexicana de ingeniería química

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Ago 2011, Volume 10 Nº 2 Páginas 179 - 188

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The aim of this study was to evaluate the release of bioactive peptides encrypted in milk proteins by proteolytic activity of lactic acid bacteria in commercial fermented milks in refrigerated storage. Peptide concentration varied from 0.05 mg / ml in LF-3 (Lactobacillus casei) and LF-4 (Lactobacillus acidophilus) to 0.227 mg/ml in LF-5 (Lactobacillus casei and S. thermophilus) and LF-1 (Lactobacillus casei) after 10 days of storage, when the maximum concentration was reached. During refrigerated storage, all samples presented peptides with molecular weight lower than 14.4 kDa. The fermented milk LF-2 (Lactobacillus acidophilu s y Streptococcus thermophilus) had the highest variety of peptides, and even peptides lower than 1.4 kDa were found, some of them with aromatic amino acids, which are present in the primary structure of antihypertensive peptides. These results suggest that the concentration and diversity of peptides depends on microorganism in the fermented milk. Peptides molecular weights were compared with those reported in literature for bioactive peptides, finding similarities, thus increasing the expectations to have peptides with physiological importance in fermented milks.

El objetivo de este estudio fue determinar la concentración y el perfil de pesos moleculares de los péptidos bioactivos encriptados en las proteínas de la leche y liberados por la actividad proteolótica de bacterias lácticas en leches fermentadas comerciales, durante su almacenamiento refrigerado. La concentración de péptidos varió de 0.05 mg/ml en LF-3 (Lactobacillus casei) y LF-4 (Lactobacillus acidophilus), a 0.227 mg/ml en LF-5 (Lact obacillus casei y S. thermophilus) y LF-1 (Lactobacillus casei) en el décimo día de almacenamiento, en el cual se presentó el máximo de concentración. Durante el almacenamiento refrigerado de todas las muestras, se encontraron péptidos de peso molecular menor a 14.4 kDa. La leche fermentada con Lactobacillus acidophilus y Streptococcus thermophilus (LF-2) presentó la mayor diversidad de péptidos, y en ésta se encontraron fragmentos aún menores a 1.4 kDa, algunos de ellos con aminoácidos aromáticos, presentes en la estructura primaria de péptidos antihipertensivos. Estos resultados sugieren que la concentración y diversidad de péptidos depende del microorganismo que fermenta la leche. El peso molecular de los péptidos encontrados se comparó con los reportados en la literatura como péptidos bioactivos, y se encontraron semejanzas, lo que incrementa la probabilidad de tener péptidos de importancia fisiológica en leches fermentadas.

11716 downloads Citado 4 vezes em SciELO

11.

Concordancia de las pruebas de tuberculina e Interferón gamma en población reclusa
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Marco Mouriño, A.

; Orcau Palau, A.

; Jané Galliga, R.

; Escribano Ibáñez, M.

; Caylà Buqueras, J.A.

; Solé Zapata, N.

; del Baño Hollín, L.

; Quintero del Río, S.

; Ferrer Escobar, M.D.

; Mangues Bafalluy, J.

; Guerrero Moreno, R.A.

; Martín Sánchez, V.

Revista Española de Sanidad Penitenciaria

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Jun 2011, Volume 13 Nº 1 Páginas 15 - 20

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Objective: To study the agreement of Tuberculin Skin Tests (TST) and Interferon Gamma Release Assays (IGRA) when screening tuberculosis infection amongst inmates recently admitted to prison. Materials and methods: Prospective study conducted in a prison during the months of May and June 2009. Inmates without a TB history, with previous TST negatives or without prior TSTs were included. Participants signed an informed consent form and the study was approved by an independent Ethical Committee. TST (positive 10 > or = mm) and IGRA (Quantiferon TB-Gold) were performed and standardized data collection was carried out. The agreement between both tests was analysed using the Kappa index. Results: A total of 181 people were included. 62% were foreign-born, 17% had previous BCG vaccination, 8.4% were IDUs and 4% HIV-infected. Foreign born subjects were more frequently vaccinated and presented less drug use and HIV infection than people born in Spain. (p=0.02, p=0.02 and p=0.01 respectively). TST results were positive in 24% and IGRA in 26%. Both tests were performed in 149 people (82%). Discordant results were observed in 15.8%. Agreement of the Kappa coefficient was 0.6 (CI 0.4-0.7). Agreement was better in the native population (K=0.8) and worse in BCG vaccinated (K=0.4) and foreign-born subjects (K=0.8). Conclusion: Overall agreement was moderate and was less amongst vaccinated subjects and those born abroad. Extension of the study could be useful to evaluate which test better predicts the risk of progression to active TB and the cost-benefit of both tests among the prison population.

Objetivos: Estudiar en población penitenciaria la concordancia de la prueba de la tuberculina (PT) y las pruebas de interferón gamma (IFG). Material y métodos: Estudio prospectivo realizado en una prisión en mayo-junio de 2009. Se estudian los ingresos sin antecedente de tuberculosis (TB) o con PT previa negativa o no realizada. Se realizó IDR de Mantoux (positivo ³ 10 mm) y extracción sanguínea para prueba de IFG (QuantiFERON®-TB Gold). En los infectados, se realizó despistaje de TB. Se pasó un cuestionario y se solicitó consentimiento informado. El estudio fue aprobado por un Comité ético ajeno a instituciones penitenciarias. La concordancia entre PT e IFG se basó en el índice Kappa. Resultados: Se incluyeron 181 casos. El 62% eran extranjeros, el 17% vacunados por BCG, el 8,4% UDI y el 4% VIH+. En los extranjeros había más vacunados, menos UDI y menos infectados por VIH que en autóctonos (p=0,02, p=0,02, y p=0,01, respectivamente). La PT fue positiva en el 24% y la IFG en el 26%. Hubo información de ambas en 149 (82%) casos. El 15,8% fueron discordantes. El índice Kappa fue de 0,6 (0,4-0,7). La concordancia varió según subgrupos, siendo mayor en autóctonos (kappa= 0,8) y menor en vacunados (kappa=0,4) e inmigrantes (kappa=0,5). Conclusión: La concordancia global fue moderada-buena, pero en vacunados e inmigrantes fue menor. El nivel de discordancia aconseja ampliar el estudio, así como evaluar que prueba predice mejor el riesgo de progresión a TB y el coste-beneficio de ambas en la población reclusa de nuestro país.

788 downloads

12.

«RETRATO» (REgistro de TRAuma grave de la provincia de TOledo): visión general y mortalidad
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Marina-Martínez, L.

; Sánchez-Casado, M.

; Hortiguela-Martin, V.

; Taberna-Izquierdo, M.A.

; Raigal-Caño, A.

; Pedrosa-Guerrero, A.

; Quintana-Díaz, M.

; Rodríguez-Villa, S.

Medicina Intensiva

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Set 2010, Volume 34 Nº 6 Páginas 379 - 387

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Objective: To analyze the management and progression of the critical trauma patient. Design: A retrospective, descriptive analysis. Setting: The ICU in the province of Toledo. Patients: All patients with traumatic injury admitted during the 2001-2007 period (7 years). Main variables of interest: These include the variables at the scene of the accident, pre-hospitalization, during transportation, variables on admission and during development until discharge or death. Results: A total of 1090 trauma patients admitted were included. Of these, 79.5% were male, with an average age of 36.5 years (16% >65 years). There was a progressive decrease of patients from 2001 (142 patients) to 2007 (133 patients), with 46.9% admissions between May and September. A total of 29.4% did not belong to the health area. The causes were car accident (43.3%), fall from a height/fall (20.8%), motorcycle accident (13.8%), pedestrian being run over (6.6%). There were 2172 injuries; 30.1% had 3 injuries and 8.4% >4. The most frequently occurring injury was a head injury (33.7%), followed by thoracic trauma (20.2%) and orthopedic trauma (15.6%). 36.4% required surgery on the first day. Average length of stay in the ICU was 10.4±13.2 days. Time on mechanical ventilation was 7.3±12 days (median 1 day). Fifteen percent died in the ICU. This remains within the multivariable ICU mortality prediction model, including the pre-hospitalization variables: age (OR 1.05; 95% CI: 1.03-1.06), mydriasis (OR 2.6; 95% CI: 1.3-5.3), motor component of the Glascow Coma Score (GCS) (OR 0.7; 95% CI: 0.6-0.8), pre-hospitalization shock (OR 3.2; 95% CI: 1.8-5.5) and Injury Severity Score (ISS) (OR 1.1; 95% CI: 1.05-1.1). Conclusions: The use of multicenter trauma registers gives an overall view of trauma management and helps improve the care.

Objetivo: Análisis del tratamiento y de la evolución del enfermo traumático crítico. Diseño: Estudio descriptivo y retrospectivo. Ámbito: Las UCI de la provincia de Toledo. Pacientes: Todos los pacientes con enfermedad traumática ingresados durante el período 2001-2007 (7 años). Variables de interés principales: Variables en el lugar del accidente, prehospitalarias, durante el transporte; variables de ingreso hospitalario y de evolución hasta el alta o el fallecimiento. Resultados: Se incluyó a 1.090 pacientes traumáticos ingresados. El 79,5% eran varones. La edad mediana fue de 36,5 años (>65 años [16%]). El descenso fue progresivo desde el año 2001 (142 pacientes) hasta el año 2007 (133 pacientes). El 46,9% ingresó entre mayo y septiembre. El 29,4% no pertenecía al área sanitaria. Las causas fueron accidente de coche (43,3%), precipitación/caída (20,8%), accidente de moto (13,8%) y atropello de peatón (6,6%). Hubo 2.172 lesiones. El 30,1% tenía 3 lesiones y el 8,4% tenía 4 o más lesiones. La lesión más frecuente fue el traumatismo craneoencefálico (33,7%), seguido por el trauma torácico (20,2%) y el trauma ortopédico (15,6%). El 36,4% necesitó cirugía en el primer día. La estancia media en la UCI fue de 10,4±13,2 días. El tiempo de ventilación mecánica fue de 7,3±12 días (mediana de 1 día). El 15% falleció en la UCI. Permanecieron en modelo multivarible de predicción de mortalidad en la UCI incluyendo las variables prehospitalarias: edad (OR: 1,05; IC95%: 1,03-1,06), midriasis (OR: 2,6; IC95%:1,3-5,3), componente motor del Glasgow Coma Score (OR: 0,7; IC95%: 0,6-0,8), shock prehospitalario (OR: 3,2; IC95%: 1,8-5,5) e Injury Severity Score (OR: 1,1; IC95%: 1,05-1,1). Conclusiones: La utilización de registros de trauma multicéntricos da una idea global del tratamiento y permite incidir en la mejora de los cuidados.

594 downloads

13.

Ureteritis quística asociada a litiasis
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Ballestero Diego, R.

; Correas Gómez, M. A.

; Gutiérrez Baños, J. L.

; Portillo Martín, J. A.

; Valle Schaan, J. I. Del

; Roca Edreira, A.

; Villanueva Peña, A.

; Aguilera Tubet, C.

; Zubillaga Guerrero, S.

; Ramos Barseló, E.

; Campos Sañudo, J.A.

; Hidalgo Zabala, E.

Archivos Españoles de Urología (Ed. impresa)

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Nov 2009, Volume 62 Nº 9 Páginas 763 - 764

Resumo: Es | Texto: Es | PDF: Es

475 downloads

14.

Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil
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Sabatini, Gustavo A.

; Ribolla, Paulo E. M.

; Barros, Antonio T. M.

; Guerrero, Felix D.

; Schumaker, Terezinha T. S.

Revista Brasileira de Parasitologia Veterinária

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Set 2009, Volume 18 Nº 3 Páginas 8 - 14

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Com o objetivo de verificar a ocorrência e determinar a frequência da mutação kdr (knock down resistance) em populações de Haematobia irritans (mosca-dos-chifres) resistentes aos piretróides, foram analisados 1.804 indivíduos de 37 populações de todas as Regiões do Brasil. Com exceção da Região Nordeste, o kdr (knock down resistance gene) foi encontrado em populações de todas as regiões. A mutação não foi detectada em 87,08% dos indivíduos. Entretanto, o gene foi amplificado de 12,92% das moscas, das quais 11,70% se mostraram heterozigotas resistentes e 1,22% homozigotas resistentes. Em todas as populações verificou-se equilíbrio de acordo com a Lei de Hardy e Weinberg, exceto uma com excesso de heterozigotos. Entretanto, quando agrupamos diferentes populações numa metapopulação de acordo com a região geográfica, é possível observar um desvio nas populações Centro-Oeste, Sul e Sudeste, indicando isolamento populacional e que a ocorrência do kdr é provavelmente um efeito independente, talvez refletindo a estratégia de uso do inseticida de cada produtor. Apesar da resistência aos piretróides estar disseminada por todo o país, apenas 48% das populações resistentes apresentaram o kdr, e a frequência de indivíduos kdr nas populações resistentes se mostrou bastante baixa. À exceção da Região Nordeste, o mecanismo de resistência ligado ao kdr ocorre em todo o país.

To investigate the kdr (knockdown resistance) resistance-associated gene mutation and determine its frequency in pyrethroid-resistant horn fly (Haematobia irritans) populations, a total of 1,804 horn flies of 37 different populations from all Brazilian regions (North, Northeast, Central-West, Southeast, and South) were molecular screened through polymerase chain reaction (PCR). The kdr gene was not detected in 87.08% of the flies. However, the gene was amplified in 12.92% of the flies, of which 11.70% were resistant heterozygous and 1.22% were resistant homozygous. Deviation from Hardy-Weinberg equilibrium (HWE) was found only in 1 ranch with an excess of heterozygous. When populations were grouped by region, three metapopulations showed significant deviations of HWE (Central-West population, South population and Southeast population). This indicates that populations are isolated one from another and kdr occurrence seems to be an independent effect probably reflecting the insecticide strategy used by each ranch. Although resistance to pyrethroids is disseminated throughout Brazil, only 48% of resistant populations had kdr flies, and the frequency of kdr individuals in each of these resistant populations was quite low. But this study shows that, with the apparent exception of the Northeast region, the kdr mechanism associated with pyrethroid resistance occurs all over Brazil.

https://doi.org/10.4322/rbpv.01803002 1385 downloads

15.

Patrones de distribución geográfica de los mamíferos de Jalisco, México
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Ramos-Vizcaíno, Ismael

; Guerrero-Vázquez, Sergio

; Huerta-Martínez, Francisco Martín

Revista mexicana de biodiversidad

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Jun 2007, Volume 78 Nº 1 Páginas 175 - 189

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

We analyzed the patterns of distribution of mammal species richness in Jalisco State and their relationships with some environmental factors. We retrieved distribution data from several national and foreign biological collections. We used the collecting localities to generate a spatial record of distribution points. The state was divided into 159 geographic units of classification (GUC's) of 15' by 15'. We overlap the point cover, GUCs and some maps from CONABIO to create a presence-absence matrix. We analyzed the richness distribution by vegetation type and elevation. A richness gradient was observed from tropical to semiarid vegetation types and according to elevation; we observed higher richness between 1500 and 2000 m a. s. l. and lower richness between 4000 to 4500. A Bray-Curtis ordination and a classification TWINSPAN were used. They were consistent in separating two groups of species: one of them including coastal species and the other with northeastern species, reflecting a climatic gradient. The percentage of accumulated variance was 94% and precipitation, temperature, evaporation, elevation and vegetation were the most important environmental variables.

Se analizaron los patrones de distribución de la riqueza de mamíferos en el estado de Jalisco y sus relaciones con algunos factores ambientales. Se obtuvo información de registros de mamíferos de Jalisco de diferentes colecciones biológicas nacionales y extranjeras. Se utilizaron las localidades de recolecta para realizar una cobertura de puntos. El estado se dividió en 159 unidades de clasificación geográfica (UCG´s) de 15' por 15'. Se sobrepuso la cobertura de puntos, las UCG y algunos mapas de CONABIO para formar una matriz de presencia-ausencia. Se analizó la distribución de la riqueza por tipo de vegetación y altitud. Se observó un gradiente de riqueza que va desde las zonas tropicales hasta las semiáridas. Por altitud, la mayor riqueza se encontró entre los 1500 y 2000 m y la menor de los 4000 a 4500 m. Se aplicó una ordenación de Bray-Curtis y una clasificación con TWINSPAN. Ambas fueron consistentes en formar 2 grupos de mamíferos; uno con especies de la costa y el otro del noreste del estado, lo que refleja un gradiente climático. El porcentaje de variación acumulada fue del 94% y las variables del medio con mayor influencia fueron precipitación, temperatura, evaporación, altitud y vegetación.

9947 downloads Citado 1 vez em SciELO

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