Pesquisa | SciELO

ABSTRACT Objectives: To evaluate the impact of COVID-19 on clinical and surgical practice, educational activities, health and lifestyle behavior of Brazilian urology residents. Materials and Methods: A web-based survey was sent to 468 Brazilian urology residents from postgraduate years (PGY) 3 to 5 to collect data on clinical practice and training after 4 months of COVID-19. We also assessed health-related and behavior changes, rate of infection by SARS-CoV-2, deployment to the front line of COVID-19, residents’ concerns, and access to personal protective equipment (PPE). Results: Massive reductions in elective and emergency patient consultations, diagnostic procedures and surgeries were reported across the country, affecting PGY 3 to 5 alike. Most in-person educational activities were abolished. The median damage to the urological training expected for 2020 was 6.0 [3.4 – 7.7], on a scale from 0 to 10, with senior residents estimating a greater damage (P< 0.001). Educational interventions developed included online case-based discussions, subspeciality conferences and lectures, and grand rounds. Most senior residents favored extending residency to compensate for training loss and most younger residents favored no additional training (p< 0.001). Modifications in health and lifestyle included weight gain (43.8%), reduced physical activity (68.6%), increased alcoholic intake (44.9%) and cigarette consumption (53.6%), worsening of sexual life (25.2%) and feelings of sadness or depression (48,2%). Almost half were summoned to work on the COVID-19 front-line and 24.4% had COVID-19. Most residents had inadequate training to deal with COVID-19 patients and most reported a shortage of PPE. Residents’ concerns included the risk of contaminating family members, being away from residency program, developing severe COVID-19 and overloading colleagues. Conclusions: COVID-19 had a massive impact in Brazilian urology residents´ training, health and lifestyle behavior, which may reflect what happened in other medical specialties. Studies should confirm these findings to help developing strategies to mitigate residents’ losses.

https://doi.org/10.1590/s1677-5538.ibju.2021.99.09 170 downloads

Carcinoma sarcomatoide renal metacrónico asociado a leucemia linfocítica crónica. Reporte de un caso
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Jesús-Henriques, Leidy C. de

; Rosales-Pereira, Katherine A.

; Oliveira-Gomes, Diana de

; Iglesias-Fortes, Rocío

; Dávila-Alcalá, Erik L.

Gaceta mexicana de oncología

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2021, Volume 20 Supl. 1 Páginas 51 - 55

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract A multiple primary malignancy (MPN) is evidence of two or more histologically different malignancies presenting in the same patient. A series of cases have been reported worldwide, however, in Venezuela there are no cases or reported incidence. The following is the case of an 82-year-old patient in whom the coexistence of a renal sarcomatoid carcinoma with chronic lymphocytic leukemia, the association of which is poorly described. Early diagnosis of MPN leads to effective treatment, giving the patient better survival and quality of life.

Resumen Una neoplasia maligna primaria múltiple (NMPM) es la evidencia de dos o más tumores malignos histológicamente diferentes presentados en el mismo paciente. Mundialmente se han reportado una serie de casos, sin embargo, en Venezuela no existen casos ni incidencia reportada. A continuación, se presenta el caso de una paciente de 82 años en quien se demostró la coexistencia de un carcinoma sarcomatoide renal con leucemia linfocítica crónica, cuya asociación es poco descrita. El diagnóstico precoz de las NMPM conduce a un tratamiento eficaz, confiriendo al paciente mejor supervivencia y calidad de vida.

https://doi.org/10.24875/j.gamo.21000043 18 downloads

Impact of COVID-19 on clinical practice, income, health and lifestyle behavior of Brazilian urologists
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Gomes, Cristiano M.

; Favorito, Luciano A.

; Henriques, João Victor T.

; Canalini, Alfredo F.

; Anzolch, Karin M. J.

; Fernandes, Roni de C.

; Bellucci, Carlos H. S.

; Silva, Caroline S.

; Wroclawski, Marcelo L.

; Pompeo, Antonio Carlos L.

; Bessa Jr., Jose de

International braz j urol

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Dez 2020, Volume 46 Nº 6 Páginas 1042 - 1071

Resumo: En | Texto: En | PDF: En

ABSTRACT Objectives To evaluate the impact of COVID-19 on clinical practice, income, health and lifestyle behavior of Brazilian urologists during the month of April 2020. Materials and Methods A 39-question, web-based survey was sent to all urologist members of the Brazilian Society of Urology. We assessed socio-demographic, professional, health and behavior parameters. The primary goal was to evaluate changes in urologists’ clinical practice and income after two months of COVID-19. We also looked at geographical differences based on the incidence rates of COVID-19 in different states. Results Among 766 urologists who completed the survey, a reduction ≥ 50% of patient visits, elective and emergency surgeries was reported by 83.2%, 89.6% and 54.8%, respectively. An income reduction of ≥ 50% was reported by 54.3%. Measures to reduce costs were implemented by most. Video consultations were performed by 38.7%. Modifications in health and lifestyle included weight gain (32.9%), reduced physical activity (60.0%), increased alcoholic intake (39.9%) and reduced sexual activity (34.9%). Finally, 13.5% of Brazilian urologists were infected with SARS-CoV-2 and about one third required hospitalization. Urologists from the highest COVID-19 incidence states were at a higher risk to have a reduction of patient visits and non-essential surgeries (OR=2.95, 95% CI 1.86 – 4.75; p< 0.0001) and of being infected with SARS-CoV-2 (OR=4.36 95%CI 1.74-10.54, p=0.012). Conclusions COVID-19 produced massive disturbances in Brazilian urologists’ practice, with major reductions in patient visits and surgical procedures. Distressing consequences were also observed on physicians’ income, health and personal lives. These findings are probably applicable to other medical specialties.

https://doi.org/10.1590/s1677-5538.ibju.2020.99.15 3190 downloads

Study of Thermoplastic Extrusion and Its Impact on the Chemical and Nutritional Characteristics and Two Sorghum Genotypes SC 319 and BRS 332
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Campelo, Flávia A.

; Henriques, Gilberto S.

; Simeone, Maria Lucia F.

; Queiroz, Valéria A. V.

; Silva, Mauro R.

; Augusti, Rodinei

; Melo, Júlio O. F.

; Lacerda, Inayara C. A.

; Araújo, Raquel L. B.

Journal of the Brazilian Chemical Society

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Abr 2020, Volume 31 Nº 4 Páginas 788 - 802

Resumo: En | Texto: En | PDF: En

The use of sorghum in human nutrition has been expanded due to its nutritional composition and its functional potential. Extrusion has been used to improve the quality of sorghum. The aim of this work was to assess the effect of extrusion in the nutritional composition, phenolic compounds content of two sorghum, and characterize the profile of chemical constituents by paper spray mass spectrometry (PS-MS). Extrusion increased the carbohydrate and fiber contents and reduced moisture, lipids and ashes, and enabling a higher degree of liberation of phenolic compounds and antioxidant activity. The fingerprint obtained in both ionization modes had been influenced by extrusion, allowing for a greater compounds’ identification on the extruded samples. The analysis of sorghum samples’ main components made distinguishing of the main constituents possible, as well as highlighted the extrusion effect and the influence of the genotypes. This study demonstrated that extrusion enabling a higher liberation of phenolic compounds, which were identified by the PS-MS technique.

https://doi.org/10.21577/0103-5053.20190243 389 downloads

ENZYMATIC BIODIESEL SYNTHESIS FROM ACID OIL USING A LIPASE MIXTURE
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Pedro, Kelly C. N. R.

; Parreira, Juliana M.

; Correia, Igor N.

; Henriques, Cristiane A.

; Langone, Marta A. P.

Química Nova

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Mar 2018, Volume 41 Nº 3 Páginas 284 - 291

Resumo: En | Texto: En | PDF: En

The conventional biodiesel production process has some disadvantages. It is necessary to use refined vegetable oils with low free fatty acids (FFAs) content. An alternative route is to use low-cost acid oils in an enzymatic process. The use of lipases allows simultaneous esterification of FFAs and transesterification of triglycerides present in raw material forming alkyl esters. The aim of this work was to study the production of biodiesel using soybean oils with different acid contents (Acid Value of 8.5, 50, 90) and ethanol catalyzed by commercial immobilized lipases (Novozym 435, Lipozyme RM IM and Lipozyme TL IM). A significant decrease of acid value was observed mainly with Novozym 435 and Lipozyme RM IM. The use of a mixture of two immobilized lipases was also investigated to decrease catalyst cost and increase the amount of ester produced. The three commercial immobilized lipases were mixed in a dual system and tested for biodiesel synthesis from acid oil (AV of 8.5, 50 and 90). A positive synergistic effect occurred mainly for Lipozyme TL IM (1,3-specific lipase) and Novozym 435 (non-specific lipase) blend. The ester content doubled when this lipase mixture was used in ethanolysis of acid oil with AV of 90.

https://doi.org/10.21577/0100-4042.20170180 1870 downloads

Fibroblastoma desmoplásico envolvendo a artéria braquial: um caso clínico único
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Coelho, Henriques

; Meira, J.

; Martins, V.

; Campos, J.

; Coelho, A.

; Augusto, R.

; Semião, C.

; Pinto, E.

; Canedo, Alexandra

Angiologia e Cirurgia Vascular

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Dez 2017, Volume 13 Nº 4 Páginas 46 - 49

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O fibroblastoma desmoplásico (FD), também conhecido como fibroma colagenoso, é uma neoplasia fibrosa benigna rara, de crescimento indolente, recentemente descrita. Ocorre predominantemente no tronco e membros superiores, poden- do surgir em qualquer idade. Os autores descrevem um caso de uma doente do sexo feminino de 42 anos de idade com FD, com compromisso neurovascular do membro superior. Foi submetida a exérese em bloco da massa tumoral e artéria braquial, com reconstrução arterial (enxerto de interposição autólogo). A análise anatomopatológica foi consistente com FD e o diagnóstico foi validado por laboratório externo internacional1. Trata-se da primeira descrição na literatura de um FD envolvendo um segmento arterial.

Desmoplastic fibroblastoma (DF), also known as collagenous fibroma, is a rare benign fibrous tumor, recently described. It occurs predominantly in trunk and upper limbs, and can appear at any age. The authors describe a case of a 42-year- old female patient with DF, with upper limb neurovascular compromise. Patient was submitted to block excision of the tumor mass and brachial artery, with arterial reconstruction (autologous interposition graft). The anatomopathological analysis was consistent with DF and the diagnosis was validated by an international external laboratory1. This is the first description in the literature of an DF involving an arterial segment.

723 downloads

Development and validation of a stability indicating method for quantification of the sesquiterpene lactone eremantholide C from Lychnophora trichocarpha (Brazilian arnica)
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Henriques, Bárbara O.

; Saúde-Guimarães, Dênia A.

; Caldeira, Tamires G.

; Souza, Jacqueline de

Revista Brasileira de Farmacognosia

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Ago 2017, Volume 27 Nº 4 Páginas 502 - 509

Resumo: En | Texto: En | PDF: En

ABSTRACT Pharmacological activities as anti-inflammatory, anti-hyperuricemic, anti-gouty arthritis, antitumor and trypanocidal activities of the aerial parts from Lychnophora trichocarpha (Spreng.) Spreng. ex Sch.Bip., Asteraceae (Brazilian arnica) have already been proved. Eremantholide C is a sesquiterpene lactone and one of the active chemical constituents responsible for these activities presented by L. trichocarpha. Therefore, the aim of this work was to develop and validate a stability indicating HPLC method for eremantholide C. Eremantholide C stability was evaluated in L. trichocarpha ethanolic extract and in its isolated form. Analytical conditions employed C18 column, acetonitrile/water in gradient elution, flow of 0.8 ml/min at 30 ºC. To correct for the loss of analyte during sample preparation the use of coumarin as an internal standard was necessary. The developed method provides good separation and resolution of the peaks, allowing quantification of eremantholide C, isolated or directly in the ethanolic extract, in internal standard presence. Validation results showed that this method is linear in the concentration range 2–180 µg/ml, precise, accurate and specific. Stability studies showed that L. trichocarpha ethanolic extract and eremantholide C remain stable for 6 months when stored at room temperature and impermeable glass bottle, therefore they can be used safely and effectively within this period. While at 40 ºC there was stability loss, at 8 ºC a stability increase was observed for the extract and the isolated eremantholide C. Forced degradation studies showed that eremantholide C degraded under acidic and alkaline conditions and was stable for three days under neutral and oxidative conditions, and when exposed to high temperature. Thus, with the development of a stability indicative method and the application of it in eremantholide C stability studies, the results can guide the development of new products that adequately preserve the original features of the biologically active substance with quality, safety and efficacy.

https://doi.org/10.1016/j.bjp.2017.03.001 617 downloads

Variation in male reproductive system characters in Corydoradinae (Loricarioidei: Callichthyidae) reflects the occurrence of different lineages in this subfamily
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Spadella, Maria A.

; Desan, Simone P.

; Henriques, Teresa C. B. P. O.

; Oliveira, Claudio

Neotropical Ichthyology

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2017, Volume 15 Nº 1 elocation e160039

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A família Callichthyidae compreende um grupo monofilético bem corroborado, dividida em duas subfamílias: Corydoradinae e Callichthyinae. Uma proposta recente, baseada em dados moleculares, sugeriu que a subfamília Corydoradinae é composta por nove linhagens, possivelmente gêneros. As espécies pertencentes a cada uma destas linhagens possuem extensivas modificações no tamanho do genoma, incluindo espécies diplóides, tetraplóides e octaplóides. Considerando a ocorrência dessas diferentes linhagens e que as extremas variações em conteúdo de DNA podem implicar em alterações significativas na estrutura dos espermatozoides, o presente estudo analisou a morfologia do sistema reprodutor masculino e a morfometria da cabeça dos espermatozoides de representantes das nove linhagens de Corydoradinae, procurando características particulares em cada uma. Os dados morfológicos revelaram a ocorrência de grande variação dentro das linhagens, maior que aquela observada entre espécies de diferentes linhagens. Diferentemente, os dados morfométricos obtidos para oito das nove linhagens revelaram grande congruência com a atual proposta para Corydoradinae. Estes resultados demonstram que há correlação entre as variações em conteúdo de DNA e o tamanho da cabeça dos espermatozoides, fornecendo, assim, subsídio adicional para a definição das nove linhagens de Corydoradinae.

ABSTRACT Callichthyidae comprises a well-corroborated monophyletic group divided into two subfamilies: Corydoradinae and Callichthyinae. A recent proposal, based on molecular data, suggests that Corydoradinae is composed by nine monophyletic lineages, possibly genera. The species pertaining to those lineages have extensive modification in the size of genome, including diploid, tetraploid and octoploid species. Considering the occurrence of these monophyletic lineages and that the variations in DNA content may imply in significant alterations on the structure of spermatozoa, this study analyzed the morphology of the male reproductive system and the morphometry of the head of the spermatozoa of representatives of the nine lineages of Corydoradinae, seeking for particular characteristics of each lineage. Morphological data revealed a high intra-lineage variation, larger than that observed among species of different lineages. In contrast, morphometric data obtained for eight out of the nine lineages, revealed large congruency with the hypothesis that Corydoradinae is composed by different lineages. These results demonstrate that there is a correlation among variations in DNA content and the size of the spermatozoon head, thus providing additional subsides for the definition of the Corydoradinae lineages.

https://doi.org/10.1590/1982-0224-20160039 1988 downloads

Meat quality of Santa Inês and F1 Santa Inês X Dorper Lambs
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Costa, Rafael S.

; Henriques, Luciana S. V.

; Valle, Felipe R. A. Ferreira do

; Maia Júnior, Jonhny A.

; Alves, Éverton N.

; Henry, Fábio C.

; Quirino, Célia R.

; Santos Júnior, Alexandre C.

Revista de Ciências Agrárias

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Set 2015, Volume 38 Nº 3 Páginas 338 - 345

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Foram selecionados ao acaso 50 cordeiros não castrados (25 da raça Santa Inês e 25 F1 Santa Inês x Dorper) para a avaliação da cronologia dentária, peso vivo ao abate, altura de cernelha e de garupa, comprimento corporal e perímetro torácico. Após a sangria, analisou-se a temperatura e pH em diferentes intervalos de tempo (4h; 12h e 24h) nos músculos Semitendinosus e Triceps brachii e força de corte em 48h no músculo Semitendinosus. Paralelamente, foi realizada a correlação entre a análise sensorial e a análise instrumental desse músculo. A temperatura da câmara fria variou de 12,2°C às 4hs a -0,5°C às 24hs e a temperatura média das carcaças foi de 26,80°C e -0,20°C, respectivamente. O pH médio inicial do músculo Semitendinosus foi de 6,62 às 4 hs e o final 5,64 às 24 hs enquanto no músculo Triceps brachii foi de 6,50 às 4hs e 5,68 às 24hs. No músculo Semitendinosus a força de corte foi semelhante entre cordeiros da raça Santa Inês e F1 Dorper x Santa Inês, demonstrando que o grupo genético não influenciou a tenrura da carne. O painel sensorial confirmou os resultados obtidos na análise instrumental. Na correlação da análise instrumental (força de corte) com a análise sensorial, quando comparados os diferentes grupos genéticos, observou-se uma elevada correlação inversa (r = -0,87). Os valores médios para o comprimento corporal e perímetro torácico não apresentaram diferenças significativas entre os grupos genéticos.

Fifty intact ovine males were randomly selected, 25 of the Santa Inês breed and 25 F1 Santa Inês x Dorper, to evaluate the dental chronology, live weight at slaughter, withers height, hip height, body length and thoracic circumference. After exsanguination, temperature and pH were measured at different times (4h; 12h and 24h) in the Semitendinosus and Triceps brachii muscles, as well as the shear force (48h) of Semitendinosus muscle. In parallel, the correlations between sensory analysis and instrumental analysis values for these muscles were assessed. The mean temperatures of the carcasses were 26.80°C and -0.20°C, respectively. The mean initial pH of Semitendinosus was 6.62 (4h) and the final was 5.64 (24h), whereas the initial pH for the Triceps brachii was 6.50 (4h) and the final, 5.68 (24h). Semitendinosus muscle shear force was similar for lambs of Santa Inês breed and F1 Dorper x Santa Inês breed, demonstrating that the genetic group did not affected meat tenderness. The sensory panel confirmed the instrumental analysis results. When different genetic groups were compared, a high inversed correlation between the instrumental analysis (shear force) and the sensory analysis was obtained (r = -0.87). The mean for body length and thoracic circumference levels did not show a significant difference for different genetic groups.

1469 downloads

10.

Update of the Brazilian floristic list of Algae and Cyanobacteria
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Menezes, Mariângela

; Bicudo, Carlos E. M.

; Moura, Carlos W. N.

; Alves, Aigara M.

; Santos, Alana A.

; Pedrini, Alexandre de G.

; Araújo, Andréa

; Tucci, Andrea

; Fajar, Aurelio

; Malone, Camila

; Kano, Cecília H.

; Sant'Anna, Célia L.

; Branco, Ciro Z.

; Odebrecht, Clarisse

; Peres, Cleto K.

; Neuhaus, Emanuel B.

; Eskinazi-Leça, Enide

; Aquino, Eveline

; Nauer, Fabio

; Santos, Gabriel N.

; Amado Filho, Gilberto M.

; Lyra, Goia M.

; Borges, Gyslaine C.P.

; Costa, Iara O.

; Nogueira, Ina de S.

; Oliveira, Ivania B.

; Paula, Joel. C.de

; Nunes, José M. de C.

; Lima, Jucicleide C.

; Santos, Kleber R.S.

; Ferreira, Leandro C.

; Gestinari, Lísia M.S.

; Cardoso, Luciana S.

; Figueiredo, Marcia A.O.

; Silva, Marcos H.

; Barreto, Maria B.B.B.

; Henriques, Maria C.O.

; Cunha, Maria da G.G.S.

; Bandeira-Pedrosa, Maria E.

; Oliveira-Carvalho, Maria F.

; Széchy, Maria T.M.

; Azevedo, Maria T.P.

; Oliveira, Mariana C. de

; Cabezudo, Mariê M.

; Santiago, Marilene F.

; Bergesh, Marli

; Fujii, Mutuê T.

; Bueno, Norma C.

; Necchi Jr., Orlando

; Jesus, Priscila B.

; Bahia, Ricardo G.

; Khader, Samir

; Alves-da-Silva, Sandra M.

; Guimarães, Silvia M.P.B.

; Pereira, Sonia M.B.

; Caires, Taiara A.

; Meurer, Thamis

; Cassano, Valéria

; Werner, Vera R.

; Gama Jr., Watson A. da

; Silva, Weliton J. da

Rodriguésia

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2015, Volume 66 Nº 4 Páginas 1047 - 1062

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Apresenta-se uma síntese atualizada de informações sobre algas no Brasil objetivando refinar os dados reunidos até o presente, bem como avaliar os avanços sobre o conhecimento da diversidade de algas no país desde a publicação do Catálogo de Plantas e Fungos do Brasil. Os resultados de 2015 mostraram um acréscimo de 1.250 espécies (35.7%) a um total de 4.747 em relação a 2010. As classes mais diversas em número de espécies foram Bacillariophyceae, Conjugatophyceae, Florideophyceae, Cyanophyceae, Dinophyceae e Euglenophyceae. Bacillariophyceae e Cyanophyceae tiveram o maior acréscimo de espécies no intervalo de cinco anos. A região Sudeste e Sul foram as mais diversas, porém, as regiões Nordeste com os estados do Piauí e Sergipe e Centro-Oeste com os estados de Mato Grosso, Goiás e Distrito Federal destacaram-se no cenário da biodiversidade nacional. Apesar da escassez de taxonomistas e da infraestrutura limitada, os resultados obtidos evidenciaram um avanço significativo no conhecimento da diversidade de algas no país nesse período de cinco anos, iniciando uma mudança quanto as diferenças geográficas regionais.

Abstract An updated synthesis of cyanobacteria and algae information is presented for Brazil aiming to refine the data gathered to date and evaluate the progress of the biodiversity knowledge about these organisms in the country since the publication of the Catálogo de Plantas e Fungos do Brasil. The results of 2015 showed an increase of 1,250 species (35.7%) when compared to 2010, reaching a total of 4,747 species. The most diverse classes in species number were the Bacillariophyceae, Conjugatophyceae, Florideophyceae, Cyanophyceae, Dinophyceae and Euglenophyceae. Bacillariophyceae and Cyanophyceae had the highest increase in species number in the five-year interval. The Southeast and South regions were the most diverse, however, the Northeast, with the states of Piauí and Sergipe, and the Central-west region, with Mato Grosso, Goiás and Distrito Federal, also stood out in the national algal biodiversity scenario. Despite the shortage of taxonomists and limited infrastructure, the results showed a significant improvement in the knowledge regarding the diversity of cyanobacteria and algae in the country during the study period, starting to even out regional geographical differences caused by subsampling.

https://doi.org/10.1590/2175-7860201566408 4865 downloads

11.

Produção de cebola cultivada sob diferentes densidades de plantio
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Henriques, Gabrielly P. de S. A.

; Grangeiro, Leilson C.

; Paulino, Renan da C.

; Marrocos, Saulo de T. P.

; Sousa, Valdívia F. L. de

; Ribeiro, Rayanne M. P.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Jul 2014, Volume 18 Nº 7 Páginas 682 - 687

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Propôs-se, neste trabalho, avaliar o desempenho agronômico da cebola em diferentes densidades de plantio, em Mossoró, RN. O delineamento experimental utilizado foi em blocos casualizados completos em esquema fatorial 2 x 5, com quatro repetições. Os tratamentos consistiram da combinação de duas cultivares de cebola (Vale Ouro IPA 11 e Bella Dura) e cinco densidades de plantio (80, 120, 160, 200 e 240 plantas m-2). As características avaliadas foram: classificação dos bulbos em função do diâmetro transversal; produtividade total, comercial e não comercial; massa média dos bulbos; massa seca dos bulbos e relação do formato de bulbos. A cultivar mais produtiva foi a IPA 11, com produtividade total de 26,76 t ha-1. A densidade que proporcionou maior produtividade total (30,48 t ha-1) foi a de 204 plantas m-2 e comercial (24,74 t ha-1) foi a de 161 plantas m-2. O incremento da densidade reduziu a massa média e seca de bulbo, percentagem de bulbos das classes 2 e 3, mas aumentou a produtividade não comercial, percentagem de bulbos classe 1 (refugo) e relação de formato de bulbo. A cultivar Vale Ouro IPA 11 foi superior ao híbrido Bella Dura com relação à produtividade total de bulbos, massa média de bulbos comerciais e relação de formato, independentemente da densidade de plantio.

The aim of this study was to evaluate the agronomic performance of onions in different planting densities in Mossoró, RN. The experimental design used was a complete randomized blocks in factorial scheme 2 x 5, with four replications. The treatments consisted of the combination of two onion cultivars (Vale Ouro IPA 11 and Bella Dura) and five planting densities (80, 120, 160, 200 and 240 plants m-2). The evaluated characteristics were: classification of bulbs according to the transverse diameter; total, commercial and noncommercial productivity; mean mass of bulb, dry mass of bulb and relative format of bulbs. The most productive cultivar was IPA 11, with total productivity of 26.76 t ha-1. The density that provided the greatest total productivity (30.48 t ha-1) was the 204 plants m-2 and commercial (24.74 t ha-1) was the 161 plants m-2. The increase in density reduced the average of medium and dry bulb mass, bulbs percentage of classes 2 and 3 as well as increased non-commercial yield, percentage of bulbs class 1 (waste) and relation of bulb shape. The cultivar Vale Ouro IPA 11 was superior to the hybrid Bella Dura regarding total bulb yield, average weight of commercial bulbs and format relations, regardless of plant density.

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12.

A importância dos biofilmes nas infeções nosocomiais: O estado da arte
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Henriques, A.

; Vasconcelos, C.

; Cerca, N.

Arquivos de Medicina

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Fev 2013, Volume 27 Nº 1 Páginas 27 - 36

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

As infeções nosocomiais são uma realidade constante no ambiente hospitalar. São responsáveis por um elevado número de casos de infeções e são notoriamente difíceis de erradicar. Um dos motivos pelos quais o tratamento das infeções nosocomiais é difícil deve-se ao facto de muitas destas infeções serem causadas por biofilmes microbianos. Os biofilmes podem ser definidos como comunidades de micro-organismos que vivem aderidos a uma superfície e envoltos numa complexa mistura de compostos tais como proteínas, polissacáridos e DNA extracelular. O crescimento de micro-organismos sob a forma de biofilmes dificulta a sua erradicação, pois estas estruturas podem ser consideradas adaptações dos micro-organismos, de forma a continuar no hospedeiro. É especialmente relevante ter em consideração as infeções causadas por biofilmes no contexto hospitalar, pois podem necessitar de abordagens diferentes para a sua erradicação. Este artigo de revisão tem como objetivo reunir o conhecimento existente do papel dos biofilmes microbianos nas infeções nosocomiais.

Nowadays nosocomial infections are a reality in all hospital environments. They are the cause of a high number of infections, morbidity and mortality, and are recognised as being notoriously difficult to eradicate. One of the reasons for the problems in the treatment of nosocomial infection is the fact that many of the pathogens involved in this infections form biofilms. Biofilms are normally defined as communities of microorganisms adhered to a surface and surrounded by a polymeric matrix of extracellular components, such as proteins, polysaccharides and e DNA. The growth of microorganisms in biofilms makes their eradication difficult as this type of growth can be considered an adaptation of the pathogens in order to improve their persistence in the host. As such it is of special interest to account for the role of biofilms in nosocomial infections so that the appropriate approach can be taken in order to eradicate them. The aim of this article is to review the existing information on the prevalence of nosocomial infections potentially caused by biofilms.

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13.

Potent antileukemic action of naphthoquinoidal compounds: evidence for an intrinsic death mechanism based on oxidative stress and inhibition of DNA repair
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Cavalcanti, Bruno C.

; Cabral, Igor O.

; Rodrigues, Felipe A. R.

; Barros, Francisco W. A.

; Rocha, Danilo D.

; Magalhães, Hemerson I. F.

; Moura, Dinara J.

; Saffi, Jenifer

; Henriques, João A. P.

; Carvalho, Tatiane S. C.

; Moraes, Manoel O.

; Pessoa, Cláudia

; Melo, Isadora M. M. de

; Silva Júnior, Eufrânio N. da

Journal of the Brazilian Chemical Society

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Jan 2013, Volume 24 Nº 1 Páginas 145 - 163

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O presente estudo descreve a acentuada atividade citotóxica da nor-β-lapachona, seus derivados arilamino substituídos, naftoquinonas iodadas e metilada, além de nor-β-lapachonas 1,2,3-triazólicas, contra quatro linhagens de células de leucemia humana (HL-60, K562, Molt-4 e Jurkat). Nor-β-lapachonas arilamino substituídas foram identificadas com potente atividade, revelando-se como potenciais protótipos contra as linhagens tumorais descritas. Estudos utilizando o ensaio cometa evidenciaram danos ao ácido desoxirribonucleico (ADN) causado pelos derivados arilamino substituídos devido o aumento dos níveis intracelulares de espécies reativas de oxigênio (ERO's). Células de HL-60 foram selecionadas para a continuidade dos estudos de mecanismos moleculares subjacentes e apoptose induzida pelos derivados quinoidais foi observada por análise de citometria de fluxo. Cepas de Saccharomyces cerevisiae foram utilizadas para uma investigação preliminar sobre o mecanismo de ação em topoisomerases de ADN. Os estudos sugerem que, aparentemente, a citotoxidade dos compostos não envolve a inibição de topoisomerases, mas que o tratamento prejudica a atividade de reparação do ADN, provocando assim a morte celular. A capacidade em induzir apoptose e aberrações cromossômicas em fibroblastos de pulmão de hamster chinês (células V79) também foi investigada. Núcleos apoptóticos foram observados e nossos estudos indicam uma correlação entre dano ao ADN e apoptose.

The current study describes that nor-β-lapachone and its arylamino derivatives, iodinated and methylated naphthoquinones and nor-β-lapachone-based 1,2,3-triazoles exhibited pronounced cytotoxic effects against four human leukemia cell lines (HL-60, K562, Molt-4 and Jurkat). Nor-β-lapachones arylamino substituted with potent activity were identified, revealing themselves as potential prototypes against tumor cell lines. Moreover, cells treated with these compounds showed DNA damage according to the standard comet assay, a finding that was, at least in part, due to increased intracellular levels of ROS. HL-60 cells were chosen to study the underlying molecular mechanisms of cytotoxicity. Drug-induced apoptosis in HL-60 cells was observed by flow cytometry analyses. Strains of Saccharomyces cerevisiae were used for a preliminary investigation into the mechanism of drug action on DNA topoisomerases. These results suggested that the cytotoxicity of these compounds apparently does not involve topoisomerase inhibition, but that treatment impairs DNA repair activity, thus triggering cell death. Considering their pro-oxidant properties, we investigated the ability of these compounds to induce apoptosis and chromosomal aberrations as micronuclei in Chinese hamster lung fibroblasts (V79 cells). Morphological apoptotic nuclei and micronuclei induction following drug treatment were observed, suggesting a correlation between DNA damage and apoptosis.

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14.

Medicinal plants at the Sítio do Gois, Apodi, Rio Grande do Norte State, Brazil
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Paulino, Renan da C.

; Henriques, Gabrielly P. de S. A.

; Moura, Olga N. S.

; Coelho, Maria de Fatima B.

; Azevedo, Rodrigo A. B.

Revista Brasileira de Farmacognosia

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Fev 2012, Volume 22 Nº 1 Páginas 29 - 39

Resumo: En | Texto: En | PDF: En

Rural communities have accumulated knowledge about survival techniques in the midst of difficulties, such as ecological, economic or social, and one of the transformation spaces and extraction of resources to these communities is the natural vegetation and homegarden. The aim of this study was to list medicinal plants and characterize the use of these plants at the Sítio do Gois, Apodi-RN, Brazil. Open-interview, semi-structured and guided tours by local experts (field guide person, foresters, herb doctors, healers, farmers, housewives) were realized. Medicinal plants are distributed in 34 families, 46 genera and 52 species. The family with the greatest number of individuals was Lamiaceae (seven species). The species most mentioned were Amburana cearensis (77%) followed by 69% de citation of Myracrodruon urundeuva, Sideroxylon obtusifolium, Ximenia americana. Informants are aware of specific uses of native medicinal plants of the caatinga and homegardens. The most common preparations are teas and syrups, used part is the leaf and stem bark and great number of citations for disorders of the digestive and respiratory systems. The most important species with greater use agreement were A. cearensis M. urundeuva, S. obtusifolium and X. americana they deserve pharmacological studies and validation.

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15.

Intralaboratory validation, comparison and application of HPLC-UV-DAD methods for simultaneous determination of benzalkonium chloride, chlorexidine digluconate and triclosan
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Chiapetta, Simone C.

; Oliveira, Érika C. B. de

; Olivier, Beta C.

; Mercante, Luiza A.

; Henriques, Danielle M.

; Pereira Netto, Annibal D.

Journal of the Brazilian Chemical Society

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Out 2011, Volume 22 Nº 10 Páginas 1913 - 1920

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Cloreto de benzalcônio (BAC), digluconato de clorexidina (CD) e triclosan (TR) são amplamente usados como agentes antimicrobianos já que são anti-sépticos e desinfetantes, dependendo da concentração. O objetivo deste trabalho foi o desenvolvimento de metodologia para a determinação rápida e simultânea de CD, TR e dos homólogos do BAC (C12, C14 e C16) por CLAE-UV-DAD. Dois sistemas isocráticos em fase reversa foram otimizados: sistema C8 - coluna SB-C8 (250 × 4,6 mm; 5 µm) e fase móvel composta por ACN e tampão de H3PO4/NaH2PO4 0,03 mol L-1, pH 2,0 (80:20, v/v); 2,0 mL min-1 e sistema CN - coluna SB-CN (150 × 4,6 mm; 5 µm) e fase móvel composta por ACN e tampão de HOAc/NaOAc 0,1 mol L-1, pH 5,0 (70:30, v/v); 2,5 mL min-1. O tempo de análise foi menor que 6 min em ambos os sistemas, permitindo alta produtividade e baixo uso de solventes. Faixas lineares com duas ordens de magnitude foram obtidas nos dois sistemas. A sensibilidade obtida no sistema C8, para as cinco substâncias foi cerca de 20% maior que no sistema CN. Ambos os métodos apresentaram precisões melhores que 4,5%, boas resoluções (R > 1,8) e altas recuperações (96 a 103%). Os limites de quantificação foram adequados para a determinação das cinco substâncias em produtos comerciais. A análise de diversos produtos comerciais indicou boa concordância entre os métodos cromatográficos e entre ele e os valores rotulados.

Benzalkonium chloride (BAC), chlorhexidine digluconate (CD) and triclosan (TR) are widely used antimicrobial agents since they are antiseptics and disinfectants depending on the used concentration. The objective of this work was the development of methodology for the fast and simultaneous determination of CD, TR and the BAC homologues (C12, C14 and C16) using HPLC-UV-DAD. Two isocratic reverse-phase systems were optimized: system C8 - column SB-C8 (250 × 4.6 mm; 5 µm) using a mobile phase composed of ACN and H3PO4/NaH2PO4 buffer 0.03 mol L-1, pH 2.0 (80:20, v/v); 2.0 mL min-1 and system CN - column SB-CN (150 × 4.6 mm; 5 µm) using a mobile phase composed of ACN and HOAc/NaOAc buffer 0.1 mol L-1, pH 5.0 (70:30, v/v); 2.0 mL min-1. In both systems the total analysis time was lower than 6 min, leading to high throughput and low production of solvent rejects. Linear ranges with two magnitude orders were found for the five substances in both systems. The sensitivity of system C8 for the five substances was around 20% greater than that of system CN. Both chromatographic methods showed overall precisions better than 4.5%, good resolutions (R > 1.8) and high recoveries (96 to 103%). The limits of quantification were adequate for the determination of the five compounds in commercial products. The analysis of several commercial products showed a good agreement between both chromatographic methods. Good agreement among measured concentrations and labeled values was also observed.

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