Abstract Nutritional balance interferes positively in obtaining high-quality seedlings of wood species. This study aimed to evaluate nitrogen fertilization during the production of L. divaricata seedlings. The experiment was conducted in a shade house located at 24°33’S and 54°04’W, in a randomized block design formed by two forms of fertilization plus a control applied during three months, totaling nine applications, with seven replicates of five seedlings each. Both exponential and conventional fertilization regimes supported the hypothesis of growth induction of L. divaricata seedlings in relation to the control during the growth phase, with exponential fertilization as a valid option to reduce initial nutrient loss by leaching.

ABSTRACT Seed deterioration is a natural and irreversible process. Nevertheless, seed priming with water and antioxidants can minimize oxidative damage in oilseeds, resulting in attenuation of seed deterioration. The objective of this assay was to evaluate seed priming on respiratory activity of Jatropha curcas submitted to accelerated aging. Seeds from two provenances (Janauba and Pedro J. Caballero) were submitted to three priming treatments (control, immersion in deionized water, and with 750 µmol L-1 of ascorbic acid) and treated for accelerated aging at 41 °C for 72 h. The results showed that the priming of J. curcas seeds promoted tolerance to accelerated aging. Primed seeds, with ascorbic acid from Janauba and deionized water from Pedro J. Caballero, resulted in a higher percentage of normal seedlings, and increased germination speed index and seed respiration. The decline of physiological quality of J. curcas seeds after accelerated aging is directly associated with a reduction in respiratory activity that is related to seed moisture content.

RESUMO A técnica de microestaquia e enraizamento ex vitro pode resultar em mudas com o sistema radicular de melhor qualidade e com maior número de raízes secundárias, quando comparada com a estaquia convencional. O objetivo deste trabalho foi avaliar o enraizamento ex vitro e a aclimatização de microestacas de erva-mate em diferentes doses do fitorregulador ácido 3-indolibutírico (AIB) e substratos. Em sala de cultivo foi avaliada diferentes doses de ácido 3-indolibutírico (0, 250, 500, 1000 e 2000 mg.L-1) e diferentes substratos utilizados puros (areia grossa, casca de arroz carbonizada, vermiculita, fibra de coco e substrato comercial). Em câmara úmida foram avaliados os mesmos substratos utilizados puros e as seguintes composições de mesmas proporções de volume: casca de arroz carbonizada + areia grossa; casca de arroz carbonizada + areia grossa + substrato comercial; casca de arroz carbonizada + substrato comercial. O enraizamento e a aclimatização ex vitro de microestacas de erva-mate podem ser realizados em câmara úmida, sendo necessário o uso de ácido 3-indolibutírico na dose de até 1250 mg.L-1. O substrato composto em iguais proporções volumétricas de casca de arroz carbonizada + areia grossa + substrato comercial proporcionou uma maior porcentagem de microestacas enraizadas.

ABSTRACT The technique of microcutting and ex vitro rooting can result in plantlets with better quality of radicular system and larger number of secondary roots, when compared with conventional cutting. The objective of this work was to evaluate the ex vitro rooting and acclimatization of holly microcuttings in different doses of phytoregulator indole-3-butyric acid (IBA) and substrate. In the growth room were evaluated different doses of indole-3-butyric acid (0, 250, 500, 1000 and 2000 mg.L-1) and different substrates used pure (coarse sand, carbonized rice husk, vermiculite, coconut fiber and commercial substrate). In humid chamber were evaluated the same pure substrates and the following combinations of the same volume ratios: carbonized rice husk + coarse sand; carbonized rice husk + coarse sand + commercial substrate; carbonized rice husk + commercial substrate. The ex vitro rooting and acclimation of holly microcuttings can be done in the humid chamber, necessitating the use of indole-3-butyric acid at a dose of 1250 mg.L-1. The substrate composed in equal proportions of carbonized rice husk + coarse sand + commercial substrate provided a higher percentage of rooted microcuttings.

Este trabalho objetivou quantificar os efeitos do manejo hídrico durante a rustificação de mudas de Handroanthus impetiginosus, por meio da termometria foliar e por parâmetros morfométricos de qualidade. Os tratamentos foram: irrigação diária (controle), a cada dois, três e quatro dias, em 60 mudas por tratamento, durante quatro semanas. As mensurações envolveram a medição da temperatura foliar (TF), os incrementos na altura, no diâmetro de colo e na massa seca radicular. As quantificações da TF ocorreram ao final do ciclo de cada manejo de irrigação e, para as demais variáveis, realizaram-se as análises no início e no final da rustificação. Mudas de H. impetiginosus irrigadas diariamente apresentaram maior incremento em diâmetro em relação às irrigadas a cada três dias e as mudas irrigadas a cada quatro dias apresentaram maior crescimento do sistema radicular e redução no crescimento aéreo. Contudo, o uso da termometria infravermelho permitiu aferir a TF em mudas de H. impetiginosus durante a rustificação, verificando que, com o prolongamento do período de estresse hídrico, houve o aumento gradativo da temperatura foliar, sendo a maior delas observada em mudas irrigadas a cada quatro dias.

The present study aimed to quantify the effects of hydric management during the hardening process of Handroanthus impetiginosus seedlings by means of foliar thermography and morphometric parameters of quality. Irrigation treatments of 60 seedlings each were carried out at the following frequencies: daily (control), and every two, three, and four days for four weeks. Measures of leaf temperature (LT), height increment, collar diameter, and root dry mass were performed. Quantification of LT occurred at the end of each irrigation management cycle and analysis of the other variables studied was performed at the beginning and end of the hardening process. Seedlings of H. impetiginosus irrigated daily presented higher diameter increment in relation to those irrigated every three days, and the seedlings irrigated every four days showed higher root system growth and reduction of aerial part growth. The use of infrared thermometry allowed LT assessment in seedlings of H. impetiginosus during hardening. It was possible to verify that the longer the water stress period, the higher the leaf temperature, with the highest temperature observed in seedlings irrigated every four days.

O objetivo deste trabalho foi avaliar o potencial de micropropagação de erva-mate (Ilex paraguariensis Saint Hilaire) a partir de plântulas assépticas oriundas de embriões zigóticos imaturos. Ápices caulinares foram inoculados em meio base, formado por ¼ da concentração dos sais do meio MS, com diferentes doses de 6-benzilaminopurina (BAP). Ápices caulinares foram enraizados em meio base com diferentes doses de ácido indolbutírico (AIB) e avaliado o efeito do AIB do meio de enraizamento in vitro na aclimatização das plantas. O meio base com 8,88µM de BAP foi o mais efetivo para a multiplicação, promovendo o maior número e comprimento de brotações adventícias. Ápices caulinares enraizaram em 30 dias de cultivo em meio base, acrescido de 7,38µM de AIB. A dose de AIB utilizada no enraizamento in vitro interfere na aclimatização das plantas. A técnica de micropropagação descrita pode ser utilizada para a produção massal de plantas de erva-mate.

The aim of this research was to evaluate the micropropagation potential of holly (Ilex paraguariensis Saint Hilaire) aseptic plantlets derived from immature zygotic embryos. Shoot tip were inoculated in a quarter-strength MS basal medium with different doses of 6-benzylaminopurine (BAP) to induce multiple shoots. Shoot tips were inoculated in a basal medium with different doses of indol-butiric acid (IBA) for in vitro rooting and to test their effects in plantlet acclimatization. The addition of 8.88µM BAP to the basal medium increased the number of adventitious shoots and shoot height. Shoot tip rooted in basal medium with 7.38µM IBA in 30 days of culture. The IBA doses of the rooting medium affect plantlet acclimatization. The described micropropagation protocol might be used to produce a high number of holly plants.