ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.

Association characteristics of amphiphilic phenothiazine drugs Fluphenazine and Trifluperazine dihydrochloride in water have been examined and their thermodynamic parameters have been calculated using conductometry. The electrical conductivity was measured as a function of concentration at various temperatures and cmc was calculated in the temperature range of 20-50°C. Thermodynamic parameters i.e. standard free energy of micellization, ΔG° , standard enthalpy of micellization, ΔH°m and standard entropy of micellization, ASm were calculated from cmc value using closed association model.

Estudaram-se os efeitos de alguns metais pesados (cromo, zinco, cobre e cádmio), da temperatura, da salinidade induzida por NaCl e do estresse hídrico induzido por PEG sobre o crescimento e a atividade da dismutase do superóxido (SOD) em Hydrilla verticillata L. Quase todos esses metais reduziram o crescimento, mas tiveram efeito modulador em baixas concentrações. Os tratamentos de salinidade induzida por NaCl, estresse hídrico e temperatura, também o reduziram. A atividade do SOD foi inibida por cromo e zinco, mas aumentada por cobre e cádmio. O estresse salino aumentou a atividade do SOD, enquanto o hídrico o diminuiu. O SOD mostrou-se termoestável, sendo a maior atividade atingida a 45ºC.

A study was made of the role of various heavy metals (chromium, zinc, copper and cadmium) together with the effects of NaCl-salinity, PEG-mediated water stress and temperature on growth and superoxide dismutase (SOD) activity in hydrilla (Hydrilla verticillata L.). Almost all metals studied reduced growth but presented a modulatory effect at lower concentrations. NaCl-salinity, water stress and temperature treatments also reduced growth. SOD activity was inhibited by both chromium and zinc but increased by copper and cadmium. NaCl-salinity increased SOD activity while water stress decreased it. SOD showed thermostability with a higher activity being recorded at 45ºC.