Abstract We here describe the first isolation and molecular typing of Leptospira borgpe-tersenii serovar Hardjo Bovis in Argentina, obtained from urine of aborted cows from abreeding herd located in Saladillo, Buenos Aires Province. The abortions occurred in coincidence with important floods with many cows presenting suspicious serological titers and subsequentseroconversion. The percentage of abortions was 3.5% of a herd of 1700 cows and the microor-ganism was isolated from 7 of the 20 urine samples obtained.

Resumen Se describe el primer aislamiento y la tipificación molecular de Leptospira borgpetersenii serovar Hardjo Bovis en Argentina, obtenido a partir de orina de vacas abortadas de unrodeo de cría ubicado en Saladillo, provincia de Buenos Aires. Los abortos coincidieron con unperíodo de importantes inundaciones, en el que varios animales presentaron títulos serológicossospechosos y posterior seroconversión. El porcentaje de abortos alcanzó el 3,5% del total delrodeo, compuesto por 1700 vacas, y se aisló el microorganismo en 7 de 20 muestras de orinaobtenidas.

Leptospirosis is an emerging infectious disease that has been identified as both a human and animal health problem worldwide. Regular outbreaks associated with specific risk factors have been reported in Argentina. However, there are no available data concerning the genetic population level for this pathogen. Therefore, the aim of this work was to describe the genetic diversity of Leptospira interrogans through the application of two molecular typing strategies: variable number of tandem repeats (VNTR) and multilocus sequence typing (MLST). For this purpose, seven reference strains and 18 non-epidemiologically related isolates from diverse hosts and Argentinean regions were analysed. Among them, nine genotypes and seven sequence types (STs), including three unreported STs, were described using VNTR and MLST, respectively. eBURST analysis demonstrated that ST37 was the most frequent and founder genotype of a clonal complex (CCs) containing STN1 and STN3, suggesting the importance of studying the serovars belonging to this CC in Argentina. The data from maximum parsimony analysis, which combined both techniques, achieved intra-serovar discrimination, surmounted microscopic agglutination test discrepancies and increased the discriminatory power of each technique applied separately. This study is the first to combine both strategies for L. interrogans typing to generate a more comprehensive molecular genotyping of isolates from Argentina in a global context.

Positive isolation of leptospires prevalence is described in naturally infected rats in the "villa" La Cava, San Isidro, Buenos Aires Province. Monthly captures, each lasting three days, were performed from December 1995 until November 1996. Species, sex and weight of rats captured were determined. The prevalence rates (leptospiras were isolated from kidneys) were 45.8% in Rattus norvegicus and 18.1% in Rattus rattus. The isolations corresponded to virulent strains of Leptospira interrogans serogroup Icterohaemorrhagiae. In Rattus norvegicus there was not significant difference among prevalence rates according to sex or weight. The high and persistent prevalence is a risk for human and animals living in the studied area.

Se describe la prevalencia de leptospiras en ratas capturadas en la villa de emergencia La Cava, San Isidro, provincia de Buenos Aires. Desde diciembre de 1995 hasta noviembre de 1996 se realizaron capturas mensuales de ratas durante tres días en cada relevamiento, a las que se les determinó especie, sexo y peso. Las tasas de prevalencia (examinados en cuanto a su infección de leptospiras por cultivo de riñón) fueron de 45.8% en Rattus norvegicus y de 18.1% en Rattus rattus. Los aislamientos correspondieron a cepas virulentas de Leptospira interrogans serogrupo Icterohaemorrhagiae. Las tasas de prevalencia de leptospiras en Rattus norvegicus no difirieron significativamente en cuanto al sexo ni al peso de los individuos capturados. La prevalencia de leptospiras fue alta y persistente a lo largo del año, a pesar de la aparente disminución de la abundancia de ratas luego del inicio de una campaña de desratización. Esto indica el alto riesgo para personas y animales residentes en el hábitat estudiado.