ABSTRACT Blue eggshells have become economically valuable due to their higher popularity among consumers when compared with eggs of other colors, particularly in East Asia. However, eggshell colors vary widely and gradually become lighter with the increase of age. Therefore, to determine the association between DNA methylation and gene expression on the molecular mechanism of eggshell color variation in Xuefeng Black-bone chickens, we collected the shell glands from chickens that produced dark blue eggshells and light blue eggshells, respectively, and performed whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-Seq) on them. The results showed that in the context of CG, DNA methylation levels were the highest compared to CHG and CHH contexts, and were negatively correlated with gene expression levels in three different gene regions in all samples, while there was no significant correlation in the contexts of CHG and CHH. Furthermore, when disregarding the location of coding genes, a total of 55 genes will show differences not only in expression levels but also in DNA methylation levels, and among the majority of differential expression genes (n=50), the DNA methylation levels in the gene body region were the highest. GO analysis and KEGG pathway analysis were performed in these genes and the results showed that the genes NR1H4 and HEPHL1, ABCA4 and ABCA12, and the pathways, cell adhesion molecular, ABC transporters, ECM-receptor interaction, and neuroactive ligand-receptor interaction, may affect the color variation of the blue eggshell of Xuefeng Black-bone chickens.

RESUMO Introdução Desde que o experimento anaeróbico Wingate foi proposto nos anos 70, ele passou a ser utilizado como um importante método de detecção para avaliar a capacidade esportiva de alta potência dos atletas, o efeito do treinamento e o método de treinamento. Com isso, ele é frequentemente usado para medir e avaliar a capacidade de trabalho anaeróbico do corpo humano. Objetivo Analisar os efeitos do treinamento intervalado de alta intensidade sobre a capacidade anaeróbica dos lutadores livres masculinos. Métodos Foram selecionados lutadores profissionais de uma faculdade de esportes, além de 30 estudantes universitários graduados em educação física em uma faculdade de esportes para comparar o teste de potência anaeróbica. Resultados Quando avaliado pelo experimento anaeróbico Wingate, o nível de valor da potência anaeróbica dos efeitos do treinamento é melhor refletido nos primeiros 10 segundos de luta livre do experimento anaeróbico. O Wingate de 30 segundos reflete principalmente o efeito do treinamento de luta livre na potência anaeróbica humana, mas a avaliação da capacidade anaeróbica desde período não foi evidenciada. Conclusão O experimento anaeróbico Wingate é um método importante para avaliar o nível de treinamento da luta livre, podendo ser utilizado como base para avaliar o efeito do treinamento e o nível da luta livre. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction Since the Wingate anaerobic experiment was proposed in the 1970s, it has come to be used as an important detection method to evaluate athletes’ high-power sport capacity, the effect of training, and the training method. Therefore, it is often used to measure and evaluate the anaerobic work capacity of the human body. Objective Analyze the effects of high-intensity interval training on the anaerobic capacity of male wrestlers. Methods Professional wrestlers from a sports college were selected, as well as 30 college students majoring in physical education at a sports college, to compare the anaerobic power test. Results When evaluated by the Wingate anaerobic experiment, the value level of anaerobic power of training effects is best reflected in the first 10 s of the wrestling anaerobic experiment. The 30 s Wingate mainly reflects the effect of wrestling training on human anaerobic power, but the evaluation of anaerobic capacity from this period was not evidenced. Conclusion The Wingate anaerobic experiment is an important method for evaluating the level of wrestling training and can be used as a basis for evaluating the effect of training and the level of wrestling. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción Desde que se propuso el experimento anaeróbico de Wingate en los años 70, comenzó a utilizarse como un importante método de detección para evaluar la capacidad deportiva de alta potencia de los atletas, el efecto del entrenamiento y el método de entrenamiento. Por ello, se utiliza con frecuencia para medir y evaluar la capacidad de trabajo anaeróbico del cuerpo humano. Objetivo Analizar los efectos del entrenamiento interválico de alta intensidad sobre la capacidad anaeróbica de los luchadores masculinos. Métodos Se seleccionaron luchadores profesionales de una escuela de deportes, además de 30 estudiantes universitarios graduados en educación física en una escuela de deportes para comparar la prueba de potencia anaeróbica. Resultados Cuando se evalúa mediante el experimento anaeróbico de Wingate, el nivel de valor de la potencia anaeróbica de los efectos del entrenamiento se refleja mejor en los primeros 10 segundos del experimento anaeróbico de lucha. El Wingate de 30 segundos refleja principalmente el efecto del entrenamiento de lucha en la potencia anaeróbica humana, pero no se evidenció la evaluación de la capacidad anaeróbica a partir de este periodo. Conclusión El experimento anaeróbico de Wingate es un método importante para evaluar el nivel de entrenamiento de lucha, y puede utilizarse como base para evaluar el efecto del entrenamiento y el nivel de lucha. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract Glansreginin A is an indicative component in walnut and is abundant in walnut meal. The aim of this study was to isolate and purify glansreginin A from the walnut meal, and to investigate the weight loss and lipid-lowering potential of glansreginin A by studying the effect of glansregin A on the proliferation of 3T3-L1 preadipocytes. Firstly, the response surface methodology was used to effectively improve the extraction yield of glansreginin A. The maximum extraction rate of glansreginin A was 0.363%, and the optimal extraction process conditions were determined. In addition, the lipid-lowering activity of glansreginin A was investigated by cell experiments. The results showed that glansreginin A could inhibit the proliferation of 3T3-L1 preadipocytes in a dose-dependent manner. And cell cycle of different groups of cells treated with glansreginin A was also measured using flow cytometry. The results showed most of the cells were blocked in G0/G1 phase and significantly decreased in S phase. These results suggest that glansreginin A could inhibit the proliferation of 3T3-L1 preadipocytes by causing cell cycle arrest. These findings provided a theoretical basis for the future research of glansreginin A and the development of slimming and fat-reducing foods.

Abstract In last years,consumers pay more attention to natural and body-friendly pigments. There is a great tendency to promote and popularize natural dyes instead of synthetics. But instability of these natural pigments has many negative impacts on application. However,melanoidins,high molecular weight brown nitrogenous polymers formed at the end of the Maillard reaction,are increasingly studied by scholars ascribe to its enormous biological activity. They are normally formed during heat processing of foods. This brown-colored compounds may be a natural pigment due to its desirable dark brown and biological activity.what’s more,its ability is superior to other natural pigments. On the other hand,this compounds’ isolation and purification are the key step for further use and biological maintenance in various kinds of industries. However,the selection of methods is mainly dependent on their structure. It is necessary to seek out some effective modification or advanced technologies to update and even take place of the traditional methods, among which a large quantity of ways about extraction is time-consuming. Hence,this review summarizes extraction methods of melanoidins,exploring the recent literature regarding the feasibility of emerging extraction methods and as a new potential natural pigment. yearsconsumers years consumers bodyfriendly body friendly synthetics application Howevermelanoidinshigh However melanoidins high reactionare reaction activity foods browncolored colored activity.whats activitywhats activity.what s what moreits handthis hand this industries Howeverthe structure timeconsuming. timeconsuming time consuming. consuming time-consuming Hencethis Hence melanoidinsexploring exploring whats activitywhat

Abstract Chemotherapy is the main treatment option for advanced osteosarcoma, which is the most common type of primary bone malignancy. However, patients develop resistance rapidly and many succumb to the disease. Niclosamide, an anthelmintic drug, has been recently identified to display potent and selective anti-cancer activity. In this work, we show that niclosamide at sub-micromolar concentrations inhibits proliferation and migration, and induces apoptosis in both parental and chemo-resistant osteosarcoma cells, with much less toxicity in normal osteoblastic cells. Interestingly, chemo-resistant osteosarcoma cells are more sensitive to niclosamide compared to parental cells. We further identify that inhibition of β-catenin is the underlying mechanism of niclosamide’s action in osteosarcoma cells. In addition, we reveal that chemo-resistant osteosarcoma cells display increased β-catenin activity compared to parental cells, which might explain the hypersensitivity of chemo-resistant cells to niclosamide. Our work provides pre-clinical evidence that niclosamide can be repurposed for treating osteosarcoma. Our findings also suggest the therapeutic value of β-catenin to overcome osteosarcoma chemo-resistance. malignancy However disease Niclosamide drug anticancer anti cancer submicromolar sub micromolar migration chemoresistant chemo resistant Interestingly βcatenin β catenin niclosamides s addition preclinical pre clinical chemoresistance. chemoresistance resistance. chemo-resistance

RESUMO: O desenvolvimento de destinos de turismo rural na China está enfrentando vários problemas, incluindo a expansão cega e um modelo de desenvolvimento repetitivo, fazendo com que o setor entre em um ciclo vicioso de competição. A percepção dos turistas sobre os destinos de turismo rural e sua vontade de revisitar os lugares fornecerão insights para os prestadores de serviços que os capacitarão a formar melhores estratégias para se destacar no mercado acirrado. Compreender a percepção do turista permitirá que os provedores contribuam para a construção de destinos turísticos rurais. Tomando a aldeia de cultura popular Ma Wei Yi como um estudo de caso, este artigo combina o status de desenvolvimento do turismo rural em Ma Wei Yi, obtém dados em primeira mão por meio de entrevistas por questionário, verifica a hipótese de pesquisa e usa o coeficiente de Pearson para realizar a análise de correlação. O artigo conclui que a percepção rural dos turistas tem um impacto positivo e forte na sua vontade de revisitar, e seus fatores têm impactos diferentes. De acordo com os resultados empíricos, os provedores de destinos turísticos devem prestar atenção ao desenvolvimento perceptivo rural em seus processos de desenvolvimento e operação de acompanhamento. Eles podem então efetivamente atrair turistas para visitar várias vezes, aumentar a sensação de experiência agradável do turista e alcançar o desenvolvimento sustentável. RESUMO problemas repetitivo competição acirrado rurais caso questionário correlação diferentes empíricos acompanhamento vezes sustentável

ABSTRACT: The development of rural tourism destinations in China is confronting multiple issues, including blind expansion and a repetitive developmental model, causing the sector to enter a vicious competition cycle. The perception among tourists of rural tourism destinations, and their willingness to revisit places will provide insights for service providers that will enable them to form better strategies to stand out in the fiercely competitive market. Understanding tourist perception will enable providers to contribute to the construction of rural tourist destinations. Taking Ma Wei Yi Folk Culture Village as a case study, this paper combines the development status of rural tourism in Ma Wei Yi, obtains first-hand data through questionnaire interviews, verifies the research hypothesis, and uses Pearson coefficient to carry out correlation analysis. The paper concluded that tourists’ rural perception has a positive and strong impact on their willingness to revisit, and its factors have different impacts. According to the empirical results, tourist destination providers should pay attention to rural perceptual development in their follow-up development and operation processes. They can effectively attract tourists to visit multiple times, enhance tourist’s sense of enjoyable experience, and achieve sustainable development. ABSTRACT issues model cycle market study firsthand first hand interviews hypothesis analysis impacts results followup follow up processes times s experience

PURPOSE: This study verifies whether the personal psychological factor of power distance disposition is perceived as conformity or obedience to the parties, triggers unethical pro-organisational behaviour (UPB) and is reinforced by hierarchy and market cultures DESIGN/METHODOLOGY/APPROACH: Structural equation model analysis was used to test the hypotheses, and Process macro was used to test the moderating effect rigorously. A survey was conducted from 15 August 2018 to 20 September 2018, and 565 questionnaires were collected for analysis FINDINGS/RESULTS: The effect of power distance orientation (PDO) on UPB was determined by deriving the regression coefficient with the control variable input in the relationship between the independent and dependent variables. Power distance orientation had a significant positive effect on UPB, even after controlling the influence of demographic variables. Pro-organisational members do not refrain from unethical actions when carrying out the tasks assigned by the company. This causal relationship is strengthened when hierarchy and market cultures are reinforced PRACTICAL IMPLICATIONS: To instil ethical behaviour in employees, a company must continuously manage the organisational culture and atmosphere, as well as educate and train employees on the company's code of ethics ORIGINALITY/VALUE: This study contributes to the limited body of knowledge examining both PDO (i.e. individual psychological factor) and organisational culture (i.e. work environment factor) as factors inducing UPB

RESUMO Introdução A ginástica aeróbica é um esporte emergente. Ela desenvolve principalmente o metabolismo aeróbico corporal aprimorando a função cardiopulmonar do praticante. Objetivo Analisar a influência no treino de fortalecimento do core sobre a aptidão física em atletas universitárias praticantes de ginástica aeróbica. Métodos Utilizando estatísticas matemáticas, estuda-se a influência do exercício aeróbico sobre a aptidão física das atletas universitárias. O teste do índice de aptidão física de atletas de aeróbica feminina de faculdades foi executado com Excel 2007 e discutiu-se os resultados obtidos. Resultados As estudantes universitárias apresentaram melhora significativa na somatização, sensibilidade interpessoal, ansiedade, e outros quatro fatores após o exercício de ginastica aeróbica (P<0,05). Após o período de prática, as estudantes universitárias tiveram melhora no desempenho ortostático, salto à distância, no alongamento em posição sentadas para frente, 1 minuto sentadas, corrida 4 vezes de ida e volta por 10 metros, e 800 metros. O aumento foi respectivamente de 11,16cm, 5,02cm, 8,34 vezes/min, 2,13s, e 9,42s. Conclusão O treino do core na ginástica aeróbica pode fortalecer o corpo, permitindo a conscientização do treino físico nas universitárias. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

ABSTRACT Introduction Aerobic gymnastics is an emerging sport. It develops mainly the aerobic body metabolism improving the cardiopulmonary function of the practitioner. Objective To analyze the influence of core strength training on the physical fitness of university athletes who practice aerobic gymnastics. Methods Using mathematical statistics, we studied the influence of aerobic exercise on the physical fitness of female college athletes. The fitness index test of female college aerobics athletes was performed with Excel 2007, and the obtained results were discussed. Results College female students showed significant improvement in somatization, interpersonal sensitivity, anxiety, and other four factors after aerobic gymnastics exercise (P<0.05). After the practice period, female college students improved orthostatic performance, long jump, stretching in sitting forward position, 1 minute sitting, running four times round trip for 10 meters, and 800 meters. The increase was 11.16cm, 5.02cm, 8.34 times/min, 2.13s, and 9.42s. Conclusion Core training in aerobic gymnastics can strengthen the body, allowing awareness of physical training in college women. Evidence Level II; Therapeutic Studies – Investigating the results.

RESUMEN Introducción La gimnasia aeróbica es un deporte emergente. Desarrolla principalmente el metabolismo corporal aeróbico mejorando la función cardiopulmonar del practicante. Objetivo Analizar la influencia del entrenamiento del core en la aptitud física en atletas universitarias que practican gimnasia aeróbica. Métodos Utilizando la estadística matemática, se estudió la influencia del ejercicio aeróbico en la aptitud física de las atletas universitarias. La prueba del índice de aptitud física de las atletas universitarias de aerobismo se realizó con Excel 2007 y se discutieron los resultados obtenidos. Resultados Las estudiantes universitarias mostraron una mejora significativa en la somatización, la sensibilidad interpersonal, la ansiedad y otros cuatro factores después del ejercicio de gimnasia aeróbica (P<0,05). Después del periodo de práctica, las estudiantes universitarias tuvieron una mejora en el rendimiento ortostático, en el salto de longitud, en el estiramiento en posición sentada hacia delante, en la posición sentada de 1 minuto, en la carrera de 4 vueltas de 10 metros y en los 800 metros. El aumento fue, respectivamente, de 11,16 cm, 5,02 cm, 8,34 veces/min, 2,13 s y 9,42 s. Conclusión El entrenamiento del core en la gimnasia aeróbica puede fortalecer el cuerpo, permitiendo la concienciación del entrenamiento físico en las estudiantes universitarias. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Objective: To gather current evidence on the impact of antipsychotics on long-term mortality in patients with schizophrenia. Methods: We systematically searched for articles in Embase, PubMed, and PsycINFO reporting the long-term mortality (follow-up > 1 year) of patients with schizophrenia who were using any antipsychotics. We then conducted multiple meta-analyses to determine differences in long-term mortality between different types of antipsychotics. Results: We identified 45 articles that provided unadjusted long-term mortality rates, including 46,171 deaths during 2,394,911 person-years. The pooled mortality rate was 9.9 (95%CI = 7.4-12.7) per 1,000 person-years. The unadjusted crude mortality rate of antipsychotic drug users was lower than that of non-users (risk ratio [RR] = 0.546, 95%CI = 0.480-0.621), first-generation antipsychotics caused higher all-cause mortality than second-generation antipsychotics (RR = 1.485, 95%CI = 1.361-1.620), and polypharmacy had better effects than monotherapy on long-term mortality (RR = 0.796, 95%CI = 0.689-0.921). As for the causes of death, heart disease and cardiovascular disease ranked highest among cause-specific mortality (5.6 per 1,000 person-years). Conclusion: Since antipsychotics had a beneficial effect on long-term mortality in schizophrenia, greater precaution should be taken with patients who do not take them. However, since disease severity, comorbidities, and other confounding factors cannot be fully controlled, further research and verification are needed.

BACKGROUND: South Africa (SA) has embarked on a process to implement universal health coverage (UHC) funded by National Health Insurance (NHI). The 2019 NHI Bill proposes creation of a health technology assessment (HTA) body to inform decisions about which interventions NHI funds will cover under UHC. In practice, HTA often relies mainly on economic evaluations of cost-effectiveness and budget impact, with less attention to the systematic, specific consideration of important social, organisational and ethical impacts of the health technology in question. In this context, the South African Values and Ethics for Universal Health Coverage (SAVE-UHC) research project recognised an opportunity to help shape the health priority-setting process by providing a way to take account of multiple, ethically relevant considerations that reflect SA values. The SAVE-UHC Research Team developed and tested an SA-specific Ethics Framework for HTA assessment and analysisOBJECTIVES: To develop and test an Ethics Framework for use in the SA context for health priority-settingMETHODS: The Framework was developed iteratively by the authors and a multidisciplinary panel (18 participants) over a period of 18 months, using the principles outlined in the 2015 NHI White Paper as a starting point. The provisional Ethics Framework was then tested with multi-stakeholder simulated appraisal committees (SACs) in three provinces. The membership of each SAC roughly reflected the composition of a potential SA HTA committee. The deliberations and dedicated focus group discussions after each SAC meeting were recorded, analysed and used to refine the Framework, which was presented to the Working Group for review, comment and final approvalRESULTS: This article describes the 12 domains of the Framework. The first four (Burden of the Health Condition, Expected Health Benefits and Harms, Cost-Effectiveness Analysis, and Budget Impact) are commonly used in HTA assessments, and a further eight cover the other ethical domains. These are Equity, Respect and Dignity, Impacts on Personal Financial Situation, Forming and Maintaining Important Personal Relationships, Ease of Suffering, Impact on Safety and Security, Solidarity and Social Cohesion, and Systems Factors and Constraints. In each domain are questions and prompts to enable use of the Framework by both analysts and assessors. Issues that arose, such as weighting of the domains and the availability of SA evidence, were discussed by the SACsCONCLUSIONS: The Ethics Framework is intended for use in priority-setting within an HTA process. The Framework was well accepted by a diverse group of stakeholders. The final version will be a useful tool not only for HTA and other priority-setting processes in SA, but also for future efforts to create HTA methods in SA and elsewhere

Abstract The objective of this study was to evaluate the chemical composition of the seed, pulp, flesh, and peel of spaghetti squash (SS) in order to assess its nutritional and health benefits. Results showed that several of the following nutrients were found in significant amounts in different parts of SS: fat, protein, amino acids like cystine, vitamins, and mineral elements like selenium in the seeds; vitamin C and trigonelline in the flesh; and calcium and tartronic acid in the peel. Analysis of the index of nutritional quality for Ca, P, K, Na, Fe, Zn, VA, VB1, VB2, VC, non-fiber carbohydrate, protein, fat, and dietary fiber revealed that the SS seed, pulp, flesh, and peel are excellent sources of protein, dietary fiber, vitamin C, and minerals. The seed was a source of several nutritional components that could improve the growth and development in children, the flesh contained components that help to maintain blood glucose stability and assist hypoglycemic function, and the peel contributes calcium and weight-loss products to help increase bone density and reduce weigh respectively. These results suggest that spaghetti squash makes a source of valuable nutraceuticals.

ABSTRACT To reduce the shortcomings of existing filtering assistance, the talc was modified by a two-step method. In the first step, the talcum powder was heated by microwave in the acid solution. In the second step, the talcum powder was stirred with Hexadecyl Trimethyl Ammonium Bromide (HTAB) solution for optimum time under an optimum temperature. The optimum temperature, time and amount of HTAB were investigated by the batch membrane filtration experiments of kaolin suspension. The filtering effects of the new talc filter aid were evaluated with the filtration rate and moisture content in filter cake. The results showed that the optimum conditions were: the amount of 0.05 mmol/L HTAB was 100 ml, the modified temperature was 80℃ and the modified time was 60 min. When the filtrate was accumulated to 25 ml, the filtration time was 13.21 s shorter than others. The membrane flux of microfiltration was the largest (0.024 L/m2·s) when new talc filter aid was added into the kaolin suspension. The talc powder was characterized by Fourier Transform Infrared Spectroscopy and X-ray diffraction, which made the experimental results more obvious. Characterization results indicated that the hydrophobicity of the talc filter aid was excellent because microwave-assisted acidification talcum powder could absorb the cationic surfactants by electrostatic interactions. Finally, by comparing talc filtration assist with diatomite filtration assist, it is concluded that the filtration performance of talc filtration assist kaolin suspension is superior to other materials.