Abstract Objective MicroRNA-29a-3p has been reported in a variety of cancers, but its role in hypopharyngeal cancer remains unclear. This study was to determine the role of microRNA-29a-3p in the occurrence and development of hypopharyngeal cancer. Methods 40 patients with hypopharyngeal cancer who underwent surgery in the Affiliated Hospital of Jining Medical University from April 2013 to November 2017 were selected for this study. The cancer tissue samples of the patients were collected, and the patients were followed up for three years. The expression of microRNA-29a-3p in tissue samples was detected by in situ hybridization with fluorescent probe, and the relationships among microRNA-29a-3p and clinicopathological factors, postoperative recurrent-metastasis, survival time were studied. Immunohistochemical was used to detect the expression of Ki67 and E-cadherin in tissue samples. Results Combined with HE staining results showed that microRNA-29a-3p expression was relatively high in non-cancer tissue cells (red blood cells and fibroblasts in tumor interstitial vessels), but was relatively low in cancer tissue and cells. According to the follow-up data of 40 patients with hypopharyngeal cancer, tumor size, T-stage, tumor differentiation, postoperative recurrent-metastasis of hypopharyngeal cancer patients were significantly negatively correlated with microRNA-29a-3p (p< 0.05). Immunohistochemica results further confirmed that microRNA-29a-3p was negatively correlated with the expression of Ki67 and E-cadherin. The survival time of patients positively related with microRNA-29a-3p expression (p< 0.05). Moreover, ROC curve analysis showed that the area under the curve of the combined detection of miRNA-29a-3p+Ki67+E-cadherin was larger than that of the single detection of the three indexes. Conclusions The expression of microRNA-29a-3p is closely related to the occurrence, development and prognosis of hypopharyngeal cancer, and it affects the proliferation and invasion. This indicates that microRNA-29a-3p serves as a therapeutic target for the occurrence and development of hypopharyngeal cancer. The evidence of study designs of this study is IV using “Oxford Centre for Evidence-Based Medicine 2011 Levels of Evidence”.

The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma.

摘要 目的 调查中国法律法规在多大程度上反映预防儿童非故意伤害的有效干预措施。 方法 我们把重点放在六种常见的致命儿童受伤原因：溺水、道路交通伤害、跌落、中毒、烧伤和窒息。我们研究了由联合国儿童基金会、世界卫生组织和欧洲儿童安全联盟建议的...

Résumé Objectif Examiner dans quelle mesure les interventions efficaces de prévention des traumatismes involontaires chez l'enfant sont reflétées dans les lois et les réglementations en Chine. Méthodes Nous nous sommes concentrés sur les six causes communes de traumatismes mortels chez l'enfant – la noyade, les accidents de la route, les chutes, l'empoisonnement, les brûlures et la suffocation. Nous avons étudié 27 interventions recommandées par le Fonds des Nations Unies pour l'enfance, l'Organisation mondiale de la Santé ou l'Alliance européenne pour la sécurité de l'enfant. Nous avons effectué une recherche sur les lois chinoises dans l'Infrastructure des connaissances nationales de la Chine et Lawyee en utilisant les mots-clés et les synonymes pour les 27 interventions. Nous avons passé...

Резюме Цель Изучить, насколько эффективные мероприятия по предотвращению неумышленного детского травматизма детей отражены в законодательных и норматив...

Objective To examine the extent to which effective interventions to prevent unintentional child injury are reflected in the laws and regulations of China. Methods We focused on the six common causes of fatal child injuries – drowning, road traffic injury, falls, poisoning, burns and suffocation. We investigated 27 interventions recommended by the United Nations Children’s Fund, the World Health Organization or the European Child Safety Alliance. We searched China National Knowledge Infrastructure and Lawyee for Chinese legislations using keywords and synonyms for the 27 interventions. We reviewed the identified legislations for statements specifying the responsible implementation department. Findings Seven national laws, nine regulations of the State Council and 46 departmental regulations were found to relate to at least one of the interventions. Although seven of the 27 internationally recommended interventions were covered by Chinese laws, 10 were not covered by any current Chinese law or regulation. None of the interventions against drowning and falls that we investigated was covered by national laws. The implementation responsibilities for effective interventions were either not specified or were assigned to multiple governmental departments in 11 or 20 legislative documents, respectively. Conclusion In Chinese laws and regulations, interventions proven to prevent major causes of unintentional child injuries are underrepresented and the associated implementation responsibilities are often poorly defined. China should include all such interventions in laws and regulations, and assign implementation responsibility for each to a single department of the national government.

ملخص الغرض دراسة مدى انعكاس التدخلات الفعالة لوقاية الأطفال من الإصابات غير المتعمدة على القوانين واللوائح في الصين. الطريقة قمنا بالتركيز على الأسباب ا...

Resumen Objetivo Examinar en qué grado contemplan las leyes y normativas de China las intervenciones efectivas para prevenir lesiones accidentales de niños. Métodos El estudio se centró en las seis causas comunes de lesiones mortales infantiles: ahogamiento, lesiones por accidentes de tráfico, caídas, envenenamiento, quemaduras y asfixia. Se investigaron 27 intervenciones recomendadas por el Fondo para la Infancia de las Naciones Unidas, la Organización Mundial de la Salud o la Alianza Europea para la Seguridad Infantil. A continuación, se realizaron búsquedas de legislaciones de China en las bases de datos China National Knowledge Infrastructure y Lawyee usando palabras claves y sinónimos de las 27 intervenciones. Por último, se revisaron las legislaciones identificadas a fin de buscar declaraciones que especificaran el departamento responsable de la aplicación. Resultados Se halló que siete leyes nacionales, nueve normativas del Consejo de Estado y 46 normativas departamentales estaban relacionadas con al menos una de las intervenciones. Aunque las leyes de China abarcaban siete de las 27 intervenciones que se recomiendan a nivel internacional, diez de ellas no estaban incluidas en ninguna normativa o ley actual de China. Las leyes nacionales no abarcaban ninguna de las intervenciones contra ahogamientos y caídas investigadas. Las responsabilidades de aplicación de intervenciones efectivas no estaban especificadas o estaban asignadas a varios departamentos gubernamentales en 11 o 20 documentos legislativos, respectivamente. Conclusión En las leyes y normativas de China, las intervenciones que han demostrado la prevención de lesiones infantiles no intencionadas están subrepresentadas y las responsabilidades de aplicación asociadas a menudo tienen una definición vaga. China debe incluir todas estas intervenciones en las leyes y normativas, así como asignar la responsabilidad de aplicación de cada una a un único departamento del gobierno nacional.

Neste trabalho é descrito um procedimento livre de solventes, limpo, simples e eficiente para a síntese de diidropirano[3,2-b]cromenodionas (onze derivados) a partir da reação de condensação de três componentes de ácido kójico, aldeídos aromáticos e dimedona, utilizando alume como catalisador reutilizável.

A simple, green and efficient solventless procedure for the synthesis of eleven dihydropyrano[3,2-b]chromenediones from the three-component condensation reaction of kojic acid, aromatic aldehydes, and dimedone using a reusable alum as catalyst is described.

A condensação de três componentes "one pot" de 3,4-metilenodioxifenol, aldeídos aromáticos, e ácido 1,3-dimetilbarbitúrico, eficientemente promovida na presença de Zr(HSO4)4, livre de solvente, produziu 10-aril-6,8-dimetil-6,10-diidro-5-oxa-6,8-diazaantra[2,3-d][1,3]dioxol-7,9-dionas. O método oferece diversas vantagens incluindo a simplicidade, facilidade e limpeza do procedimento extrativo, tempos de reação relativamente curtos e bons a altos rendimentos dos produtos.

A one-pot three-component condensation of 3,4-methylenedioxyphenol, aromatic aldehydes, and 1,3-dimethylbarbituric acid, efficiently promoted in the presence of Zr(HSO4)4 under solvent-free conditions, produced 10-aryl-6,8-dimethyl-6,10-dihydro-5-oxa-6,8-diazaanthra[2,3-d][1,3] dioxole-7,9-diones. The method offers several advantages including simple, easy and clean work-up procedure, relatively short reaction times and good to high yields of the products.

Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.

ERBB3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3), encoding a receptor of neuregulin-1 (NRG1), has been considered a functional candidate gene for schizophrenia susceptibility. In order to investigate a relationship between ERBB3 gene and schizophrenia in the Chinese population, case-control and family-based studies were carried out in 470 cases matched by controls, and in 532 family trios. Our results failed to show any evidence of significant association between the ERBB3 rs2292238 polymorphism and schizophrenia.