ABSTRACT A great challenge of scientific research with carrot in semi-arid regions is obtaining the best productive characteristics and economic conditions by optimizing the green manure amounts using spontaneous species from the Caatinga biome, such as hairy woodrose (Merremia aegyptia L.) and roostertree (Calotropis procera Ait.). Therefore, this work aimed to agronomically and economically optimize the production of carrot roots in monocropping and of their components when fertilized with equitable biomass amounts of these spontaneous species in two cropping seasons in a semi-arid environment. The experimental design was a randomized complete block design, with five treatments and five replications. The treatments consisted of equitable amounts of hairy woodrose and roostertree biomass in amounts of 16, 29, 42, 55, and 68 t ha-1 on a dry basis. In each experiment, an additional carrot treatment without fertilizer (absolute control) was planted for comparison with the other treatments to determine maximum physical and economic efficiency. Carrot fertilization was performed with the incorporation of 48.87 t ha-1 of M. aegyptia and C. procera dry biomass into the soil to obtain the maximum optimized productive efficiency (commercial productivity). The maximum optimized agroeconomic efficiency (net income) of carrot cultivation was obtained when 49.64 t ha-1 of M. aegyptia and C. procera dry biomass was added to the soil. The use of biomass from these spontaneous species from the Caatinga biome as green manure is a viable technology for tuberose producers in monocropping in semiarid environments.

RESUMO Um grande desafio na pesquisa científica com cenoura em ambiente semiárido é obter as melhores características produtivas e condições econômicas otimizando as quantidades de adubos verdes de espécies espontâneas do bioma Caatinga, como a jitirana (Merremia aegyptia L.) e a flor-de-seda (Calotropis procera Ait.). Diante disso, esse trabalho teve como objetivo otimizar agronômica e economicamente a produção de raízes de cenoura em monocultivo e de seus componentes, quando adubados com quantidades equitativas de biomassa dessas espécies espontâneas, em duas estações de cultivos em ambiente semiárido. O delineamento experimental utilizado foi em blocos completos casualizados, com cinco tratamentos e cinco repetições. Os tratamentos consistiram de quantidades equitativas de biomassa de jitirana e de flor-de-seda nas doses de 16, 29, 42, 55 e 68 t ha-1 em base seca. Em cada experimento foi plantado um tratamento adicional com cenoura sem adubo (testemunha absoluta) para efeito de comparação com os demais tratamentos para determinar a máxima eficiência física ou econômica. A adubação da cenoura foi realizada com a incorporação de 48,87 t ha-1 de biomassa seca de M. aegyptia e C. procera ao solo para obtenção da máxima eficiência produtiva otimizada (produtividade comercial). A máxima eficiência agroeconômica otimizada (renda líquida) do cultivo da cenoura foi obtida quando 49,64 t ha-1 de biomassa seca de M. aegyptia e C. procera foram adicionados ao solo. A utilização da biomassa dessas espécies espontâneas do bioma Caatinga como adubação verde é uma tecnologia viável para produtores de tuberosas em monocultivo em ambientes semiáridos.

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

ABSTRACT The use of spontaneous species from the Caatinga biome as green manure is a promising practice in increasing the productivity of agricultural systems and sustainable exploitation of the environment. This study aimed to estimate the maximum physical and economic efficiencies of radish agroeconomic characteristics in monocropping as a function of roostertree (Calotropis procera (Ait.) R. Br) biomass amounts in two cropping seasons in a semi-arid environment. The experimental design was randomized complete blocks with five treatments and five replications. The treatments consisted of different roostertree biomass amounts: 16, 29, 42, 55, and 68 t ha-1 on a dry basis, incorporated into the soil. In each block, two additional treatments were added, one without fertilization (control) and the other with mineral fertilizer, for comparison purposes with the maximum efficiency treatment. The radish cultivar planted was ‘Crimson Gigante’. The fertilization of the radish to obtain the maximum optimized productive efficiency (9.56 t ha-1) was possible with the incorporation of 50.86 t ha-1 of C. procera dry biomass into the soil. The maximum optimized agroeconomic efficiency (based on a net income of 37,641.08 R$ ha-1) of radish cultivation was obtained with 44.39 t ha-1 of C. procera dry biomass added to the soil. The rate of return and the profit margin obtained were 2.94 R$ for each real invested, with a profit margin of 62.55%. The use of biomass from the C. procera green manure is a viable technology for producers who practice radish monoculture in a semi-arid environment.

RESUMO O uso de espécies espontâneas do bioma Caatinga como adubo verde é uma prática promissora no aumento da produtividade dos sistemas agrícolas e na exploração sustentável do meio ambiente. Este trabalho teve como objetivo estimar as máximas eficiências físicas e econômicas das características agroeconômicas do rabanete em monocultivo, em função de quantidades de biomassa de flor-deseda em duas estações de cultivos em ambiente semiárido. O delineamento experimental utilizado foi em blocos casualizados com cinco tratamentos e cinco repetições. Os tratamentos consistiram de diferentes quantidades de biomassa de flor-de-seda: 16, 29, 42, 55 e 68 t ha-1 em base seca, incorporadas ao solo. Em cada bloco dos experimentos foram adicionados dois tratamentos, um sem adubação (tratamento testemunha) e outro com adubo mineral, para fins de comparação com o tratamento de máxima eficiência. A cultivar de rabanete plantada foi a Crimson Gigante. A adubação do rabanete para obtenção da máxima eficiência produtiva otimizada (9,56 t ha-1) foi possível com a incorporação de 50,86 t ha-1 de biomassa seca de C. procera ao solo. A máxima eficiência agroeconômica otimizada (baseada na renda líquida de 37.641,08 R$ ha-1) foi obtida com a quantidade de 44,39 t ha-1 de biomassa seca de C. procera adicionada ao solo. A taxa de retorno e a margem de lucro obtida foram de R$ 2,94 para cada real investido com margem de lucro de 62,55%. A utilização da biomassa do adubo verde, C. procera, é uma tecnologia viável para produtores que praticam o monocultivo de rabanete em ambiente semiárido.

RESUMO Uma das maiores dificuldades do consórcio de rabanete-alface é obter a dose ideal de adubo verde e a densidade de plantio adequada das culturas. Diante disso, o objetivo do presente trabalho foi avaliar os beneficios agroeconômicos em consórcios de rabanete-alface sob influência da adubação verde com Merremia aegyptia e Calotropis procera e densidades populacionais de alface em ambiente semiárido, em dois anos de cultivo. O delineamento experimental utilizado foi em blocos ao acaso, com os tratamentos dispostos em esquema fatorial 4x4, com 4 repetições. O primeiro fator deste esquema consistiu de quantidades equitativas de biomassa de M. aegyptia e C. procera nas doses de 20, 35, 50 e 65 t/ha em base seca, e o segundo fator de densidades populacionais de alface de 150, 200, 250 e 300 mil plantas/ha. A produção do rabanete e da alface e seus componentes, além dos indicadores agroeconômicos, índice de produtividade do sistema (SPI), coeficiente equivalente de terra (LEC) e razão equivalente monetária (MER) foram avaliados. Os maiores beneficios agroeconômicos do consórcio de rabanete-alface foram obtidos com SPI de 15,37 t/ha, LEC de 1,27 e MER de 1,30, respectivamente na combinação de 65 t/ha de biomassa de M. aegyptia e C. procera com densidade populacional de alface de 300 mil plantas/ha. A produtividade comercial máxima otimizada de raízes de rabanete no consórcio foi de 8,45 t/ha na combinação da quantidade de biomassa de 20 t/ha de M. aegyptia e C. procera e densidade populacional de alface de 300 mil plantas/ha, enquanto a produtividade máxima de folhas de alface otimizada em consórcio foi de 17,72 t/ha, na combinação de quantidade de biomassa dos adubos verdes de 65 t/ha e densidade populacional de 300 mil plantas/ha de alface. A utilização de espécies espontâneas do bioma Caatinga como adubo verde no consórcio de rabanete-alface proporciona benefícios agroeconômicos otimizados em ambiente semiárido.

ABSTRACT One of the biggest difficulties in the intercropping of radish-lettuce is to obtain the ideal dose of green manure and the adequate planting density for the crops. Therefore, the objective of the present work was to evaluate the agro-economic benefits of radish-lettuce intercropping under the influence of green manuring with Merremia aegyptia (hairy woodrose) and Calotropis procera (roostertree) and lettuce population densities in a semi-arid environment, in two cropping seasons. The experimental design used was randomized blocks, with treatments arranged in a 4x4 factorial scheme, with 4 replicates. The first factor in this scheme consisted of equitable biomass amounts of M. aegyptia and C. procera at doses of 20, 35, 50 and 65 t/ha on a dry basis, and the second factor of lettuce population densities of 150, 200, 250 and 300 thousand plants/ha. The production of radish and lettuce and its components, in addition to the agroeconomic indicators, system productivity index (SPI), land equivalent coefficient (LEC) and monetary equivalent ratio (MER) were evaluated. The greatest agro-economic benefits of the intercropped radish-lettuce were obtained with SPI of 15.37 t/ha, LEC of 1.27 and MER of 1.30, respectively, in the combination of 65 t/ha of M. aegyptia and C. procera biomass with the lettuce population density of 300 thousand plants/ha. The maximum commercial productivity of radish roots in the intercropping system was 8.45 t/ha in the combination of the biomass amount of 20 t/ha of M. aegyptia and C. procera and lettuce population density of 300 thousand plants/ha, while the maximum lettuce leaf productivity optimized in intercropping was 17.72 t/ha, in the combination of green manures biomass amount of 65 t/ha and population density of 300 thousand plants/ha of lettuce. The use of spontaneous species from the Caatinga biome as green manure in the radish-lettuce intercropping provides optimized agro-economic benefits in a semi-arid environment.

Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

ABSTRACT Alternative methods to the intensity-duration-frequency equations have been used for intense rainfall study in places with no pluviographic data. A pioneering study, by CETESB (1986), established rainfall disaggregation coefficients for Brazil; however, the use of these coefficients take into account neither the rainfall characteristics of the interest locality nor the return period influence in obtaining the precipitation intensities. This study aimed to determine rainfall disaggregation coefficients for Doce river basin; to verify the validity of the regional coefficients use for the entire basin; and to evaluate the return period influence on these coefficients. Data from 19 gauges located in the basin, with rainfall associated with durations of 10 to 1440 min and return periods of 2, 5, 10, 20, 50 and 100 years, were used to obtain the coefficients. Student's t-test was used to assess the regional applicability of these coefficients and the return period influence. The coefficients do not provide regional validity for the entire basin; therefore, regional coefficients were estimated for five hydrologically homogeneous regions, confirming the validity of these coefficients use and the influence of the return period. We conclude that the disaggregation coefficients must be specific for the homogeneous regions and for each return period.

Abstract Candida spp. is considered an important cause of healthcare-associated infections worldwide. Currently, the emergence and spread of resistant Candida isolates are being increasingly reported, making the development of new agents urgently needed. In this study, we showed the in vitro anti-Candida activity of seven synthetic 3-alkylpyridine alkaloid analogs. Among them, alkaloid 1 presented a potent antifungal effect, which was independent of its capacity of binding with the fungal membrane ergosterol or cell wall. Analog 1 showed fungistatic and fungicidal effects against C. albicans (MIC 7.8 μg/mL and MFC 62.5 μg/mL), C. glabrata, C. krusei (MIC and MFC 31.2 μg/mL), and C. tropicalis (MIC 31.2 μg/mL and MFC 125 μg/mL). The time kill-curve study showed that compound 1 has a potent fungicidal effect in vitro, eliminating C. albicans cells. Furthermore, an in vitro synergistic effect with ketoconazole was observed for compound 1. This compound also eliminated the yeast-to-hypha transition. However, it showed high cytotoxicity against mammalian cells. Taken together, these findings support the use of compound 1 as a prototype to develop new anti-Candida agents, but molecular modifications should be done to minimize the high cytotoxicity obtained.

The emergence of antibiotic resistance among microbial pathogens is a serious public health problem, resulting in a constant need of discovering new antibiotics. For this purpose, we synthetized and determined the antimicrobial activity of copper (Cu), cobalt (Co), and tin (Sn) complexes coordinated with scorpionate ligand in a tridentate mode against microorganisms of clinical importance. New complexes with the sodium hydrotris(2-mercaptothiazolyl)borate ligand were characterized by physicochemical and spectroscopic techniques (UV-vis, IR, 1H and 13C NMR). Moreover, their antimicrobial activity was evaluated using microdilution broth method. Cytotoxicity was determined in Vero cells and the selectivity index (SI) was calculated. The coordination with metals increased the ligand antimicrobial activity, which showed no activity when evaluated alone (MIC>1,080 µM). Cu-complex (1) had activity against Candida albicans and C. krusei, with MICs of 130 and 66 µM, respectively. The compounds had low toxicity on Vero cells and chemo-informatics assays corroborated a low systemic toxic effect. The SI revealed that the compound 1 was 16 times more selective for C. krusei and 8 times more selective for C. albicans in relation to Vero cells. The Cu-complex stands out as a potential prototype for the development of new antifungal agents.

Abstract Presently work was carried out the mechanical characterization of a composite of gypsum matrix reinforced with three different mass concentrations of sugarcane fibers (1 %, 2 % and 3 %), with the objective of their possible use in the production of construction elements. The analysis was made by compression and flexural strength tests. Also, through a correlation with the flexural test, it was calculated the tensile properties. With the results of the tests, it was possible to say that there is an influence of the concentration of sugarcane fibers in improving the mechanical properties of the product. The samples reinforced with 2 % of fibers had the best values for flexural, tensile and compression strength. Finally the comparison of the results obtained with some reports of the literature is carried out, and it is verified that the same ones have values approached in the resistance to the flexural and compression strength.

Resumen En este trabajo se realizó la caracterización mecánica de un material compuesto de matriz yeso reforzado con tres diferentes porcientos másicos de fibra de caña de azúcar (1 %, 2 % y 3 %), con el objetivo de su posible utilización en la fabricación de elementos de construcción. Los análisis fueron hechos a través de ensayos de resistencia a compresión y flexión. La resistencia a tracción se calculó a partir de los resultados del ensayo a flexión. De acuerdo con los resultados es posible afirmar que existe una gran influencia de los porcientos de fibra de caña de azúcar en la mejoría de las propiedades mecánicas del material compuesto, las muestras reforzadas con un 2 % de fibras fueron las que presentaron las mejores propiedades. Finalmente se realizó la comparación de los resultados obtenidos con algunos reportes de la literatura y se obtuvieron propiedades similares a otros compuestos estudiados.

Abstract In this work, vapor - liquid (VLE) equilibrium for the binary system carbon dioxide (1) + coumarin (2) at high pressures was measured by a static synthetic method using a variable-volume view cell. Experimental data were obtained in the temperature range of 318 - 338 K and pressures up to 20 MPa. Coumarin molar fraction ranged from 3.0x10-3 to 6.0x10-3. Coumarin melting point reduction at high pressures was observed. The experimental results were modeled using the Peng-Robinson (PR) equation of state with van der Waals quadratic mixing rules (vdW - QMRs), providing a good representation of the experimental phase equilibrium data. Critical properties and the acentric factor were estimated using the Constantinou and Gani method, which showed to be satisfactory on the quality of data correlation. Results indicate coumarin solubility increases with increasing phase transition pressure at a given temperature.

A new nanocomposite based on ZnCdTe semiconductor nanocrystals (NCs) synthesized in situ on reduced graphene oxide (rGO) was obtained. The heterostructure was characterized using UV-Vis emission and absorption spectroscopies, which provided evidences of the growth of the nanocrystals onto the rGO matrix. To evaluate the electrochemical performance, carbon paste electrodes modified with the nanocomposite (QD-rGO/CPE) were prepared, showing high sensitivity in the electroanalytical detection of the pesticide carbendazim. Under optimal operational conditions a calibration curve was constructed with a linear behavior in the range of 9.98 × 10-8 to 1.18 × 10-5 mol L-1, with a correlation coefficient of 0.997. The limit of detection (LOD) and the limit of quantification (LOQ) for carbendazim were 9.16 × 10-8 and 2.78 × 10-7 mol L-1, respectively. The electrode is successfully applied for the determination of carbendazim in orange juice samples.

Morinda citrifolia (noni) is found in the Atlantic and Amazonian Forest in Brazil and with recognized uses in folk medicine. However, few studies exist in its chemical characterization. The present work aims to investigate the efficiency of the sonication for extracting bioactive compounds from fruits and leaves of noni, using an experimental design of 23, whose variables were time, temperature and mass of the plant. Parallel extractions were performed using two extraction solvents (hexane and ethanol) and the same experimental design. The compounds were identified by gas chromatography coupled to mass spectrometry. The independent variable was the mass yield and all the variables studied in the sonication process were significant (p < 0.05%) for the process. Ethanol was more effective than hexane and the leaves produced higher yield and diversity of compounds than fruits. Vitamin E (tocopherol), octanoic acid, hydroxymethylfurfural, phytol and squalene were the main compounds identified.

Abstract: In order to characterize the occurrence and intensity of droughts in the Doce River Basin, as well as to develop a system for its classification, four different drought indexes were evaluated: Percent of Normal Precipitation (PNP), Deciles Method (DM), Rainfall Anomaly Index (RAI) and Standardized Precipitation Index (SPI). The indices were calculated annually, based on precipitation data from 89 rainfall stations of the Brazilian National Water Agency (ANA). Nine analysis units (AUs) were determined in the basin and the Thiessen Polygons method was used to obtain the average precipitation in the respective drainage areas. The indices were calculated for each AU and then related to the drought intensity classes. An overall classification of the indices was proposed for the drought classification system for a 30-year base period, from 1985 to 2015. The most critical hydrological years of the Doce River Basin in relation to the drought were 1994/1995, 2000/2001 and 2014/2015, the latter being the most critical of the last 30 years. The results show that the annual drought classification system proved to be efficient in the identification of events, allowing to verify that the Doce River Basin presents a severe climatic drought condition, on average, every seven years.

The use of insects to identify drugs in a cadaver has often been explored in the field of entomotoxicology. There are accurate methods; however, they require a high cost and are very time-consuming. The objective of this study was to develop two methods based on differential pulse voltammetry (DPV) and fluorescence excitation-emission matrix (EEM) spectroscopy to classify necrophagous larvae (Chrysomya megacephala, C. albiceps, Lucilia sp. and Cochliomyia macellaria) containing flunitrazepam. The voltammograms of larval extract samples were analyzed by principal component analysis (PCA), successive projection algorithm (SPA) and genetic algorithm (GA): linear and quadratic discriminant analysis (LDA and QDA). The EEM fluorescence data from larval extract samples were analyzed by 2D and parallel factor analysis (PARAFAC) with LDA. These results suggest that DPV and EEM combined with chemometrics can be used as tools for the classification of flunitrazepam in fly larvae presenting innovative applications.

RESUMO: A Zona da Mata de Minas Gerais possui uma cadeia especializada de produção de leite de cabra. O leite de cabra é superior em qualidade em comparação com o leite de outras espécies domésticas, e a demanda por leite e produtos lácteos do público tem aumentado. Os dados sobre o sistema de criação de cabras leiteiras em Minas Gerais são escassos e relativamente antigos, e essa falta de informação limita a implementação de medidas profiláticas, especialmente para a mastite, que representa o maior problema sanitário nos rebanhos leiteiros. Isso também causa problemas socioeconômicos e problemas de mercado para a criação de cabras leiteiras. O objetivo deste trabalho foi caracterizar a mastite e as bactérias associadas em cabras leiteiras na Zona da Mata de Minas Gerais. Um total de 539 cabras em lactação foi examinado e 268 amostras individuais (uma por teto) foram coletadas de animais positivos no teste da caneca de fundo escuro e/ou Califórnia Mastitis test (CMT). As culturas microbiológicas foram realizadas em meio Agar sangue e as bactérias foram submetidas a testes fenotípicos, genotípicos e testes de susceptibilidade antimicrobiana. A prevalência de mastite subclínica foi de 28,0% e a prevalência clínica foi de 2,8%. A multiplicação bacteriana foi obtida em 62,0% das amostras. Cento e oitenta e sete bactérias foram identificadas. As espécies mais identificadas foram: Staphylococcus aureus (60,4%), seguida de Staphylococcus epidermidis (9.1%,), Escherichia coli (6.9%), Staphylococcus saprophyticus (5.9%) e Staphylococcus caprae (4,3%) em ordem decrescente. As bactérias do gênero Staphylococcus apresentaram um perfil de resistência aos antimicrobianos pertencentes à classe de beta-lactâmicos - penicilina, ampicilina e oxacilina - além da tetraciclina, em contraste com os outros antimicrobianos testados. Doze por cento dos isolados apresentaram resistência múltipla a antibióticos (MDR) e foram encontrados em cinco microrregiões. Entre as bactérias com maior prevalência de MDR, 38,5% foram E. coli e 10,6% S. aureus. Os produtores da Zona da Mata de Minas Gerais são tecnificados, trabalham com raças leiteiras especializadas praticam e possuem bom manejo. No entanto, algumas medidas relacionadas à profilaxia e ao controle das doenças, como a vacinação, têm baixa adesão ou não são realizadas por falta de assistência veterinária. Este é o primeiro estudo com foco nesta região, que possui grande relevância na produção de leite de cabra no Brasil, fornecendo informações importantes que podem auxiliar na implementação de medidas de profilaxia e controle das doenças, e na manutenção de um fornecimento constante de produtos em quantidade e qualidade suficientemente adequada para a população consumidora.

ABSTRACT: The Zona da Mata of Minas Gerais has a specialized goat milk production chain. Goat milk is superior in quality compared with milk of other domestic species, and the demand for milk and milk products for the public has increased. Data on dairy goat breeding in Minas Gerais are scarce and relatively old, and this lack of information has limited the implementation of prophylactic measures, especially for mastitis, which represents the biggest sanitary problem for dairy herds. The objective of this work was to characterize mastitis and bacteria associated with it in milking goats in the Zona da Mata of Minas Gerais. It also causes socioeconomic problems and market issues for dairy goat farming. A total of 539 lactating goats were examined and 268 individual samples (one for teat) were collected from animals positive for strip cup test and/or the California Mastitis Test (CMT). Microbiological cultures were carried out on blood agar medium and the bacteria were subjected to phenotypic, genotypic and antimicrobial susceptibility tests. The prevalence of subclinical mastitis was 28.0% and the clinical prevalence was 2.8%. Bacterial multiplication was obtained in 62% of samples. One hundred eighty seven total bacteria were identified. The most common species identified was Staphylococcus aureus (60%), followed Staphylococcus epidermidis (9.1%,), Escherichia coli (6.9%), Staphylococcus saprophyticus (5.9%) e Staphylococcus caprae (4.3%). Bacteria of the genus Staphylococcus presented a profile of resistance to antimicrobials belonging to the beta-lactam class (penicillin, ampicillin and oxacillin) in addition to tetracycline, in contrast to the other antimicrobials tested. Twelve percent of multidrug resistence (MDR) was found in five microregions. Among the bacteria with the highest prevalence of MDR, 38.5% were E. coli and 10.6% were S. aureus. The producers of the Zona da Mata of Minas Gerais are technicians who work with specialized dairy breeds and practise good management. However, some measures related to prophylaxis and control of diseases, such as vaccination, have low adherence or are not performed due to a lack of veterinary assistance. This is the first study focusing on this region, which is highly prominent in goat milk production in Brazil. It provides important information that can help in the implementation of measures for the prophylaxis and control of diseases, and for maintenance of a constant supply of products in sufficient quantities and of a quality suitable for the consumer population.