The retinoschisis is a vitreoretinal dystrophy characterized by the separation of the retina layers and the formation of intraretinal cysts, In juvenile retinoschisis are described ophthalmologic alterations distinguished of the acquired retinoschisis. The objective of present paper was to conduct a study to describe the clinical features of an atypical case of juvenile retinoschisis of a male patient aged 8 who came to consultation due to a decrease of vision and the possibility of optical help. At eye examination it was visualized a schisis at macular level and peripheral with edema ob both maculae. The electroretinogram showed a decrease in the b-wave amplitude. The tomography of optical coherence demonstrated the separation of the internal retina at level of the nervous fibers and the cystic macular edema. Treatment consisted of correction and optical help to the close vision. We conclude that it is a uncommon disease and at the same time, of a atypical case where are overlapped different signs directed towards the two clinical ways of the disease, but independently that appear characteristics of acquired retinoschisis in a young patient, the treatment of visual rehabilitation with optical help remains the more prescribed to achieve a great visual quality.

La retinosquisis es una distrofia vitreorretiniana caracterizada por la separación de las capas de la retina y la formación de quistes intrarretinianos. En la retinosquisis juvenil se describen alteraciones oftalmológicas que la distinguen de la retinosquisis adquirida. Con el objetivo de describir las características clínicas de un caso atípico de retinosquisis juvenil se realizó el estudio de un paciente varón de 8 años de edad, quien acudió a consulta por disminución de la visión y la posibilidad de ayuda óptica. Al examen del fondo de ojo se visualizó una esquisis a nivel macular y periférica con edema de ambas máculas. El electrorretinograma presentó una disminución en la amplitud de la onda b. La tomografía de coherencia óptica reveló la separación de la retina interna a nivel de la capa de fibras nerviosas y el edema macular quístico. El tratamiento realizado fue la corrección y la ayuda ópticas para la visión cercana. Se concluye que se trata de una enfermedad infrecuente y, a su vez, de un caso atípico donde se imbricaron diversos signos que orientaban hacia las dos formas clínicas de la enfermedad, pero independientemente de que aparezcan características de retinosquisis adquirida en un paciente joven, el tratamiento de rehabilitación visual con ayuda óptica sigue siendo el más indicado para lograr una mayor calidad visual.

Comparou-se o desempenho de três meios de cultura na determinação das concentrações inibitórias mínimas (CIMs) de dois antifúngicos frente a 48 amostras de C. neoformans. Para anfotericina B, os meios empregados foram o Caldo RPMI 1640, indicado como referência pelo NCCLS, o Yeast Nitrogen Base (YNB-1) indicado por Ghannoum et al., o Antibiotic Medium 3 (AM3) utilizado por vários autores e o YNB-2, sugerido por nós, que é o mesmo YNB-1, todavia, adequado à técnica preconizada pelo NCCLS. Para a anfotericina B, a mais ampla faixa de CIMs (0,125-1,0 mg/ml) foi obtida no YNB-1; os intervalos de CIMs obtidos no caldo RPMI 1640 e no YNB-2 foram muito similares enquanto que o AM3 evidenciou pequenas variações nas CIMs. Para os testes com o fluconazol utilizou-se o RPMI 1640 e o YNB-1; novamente, o mais amplo intervalo de CIMs, assim como as CIMs mais elevadas, foram observados no YNB-1; as variações de CIM 50% foram de 2,0 mg/ml no RPMI 1640 e 4,0 mg/ml no YNB-1. Os resultados obtidos permitem concluir que: o RPMI 1640 suplementado com dextrose 2% permite um bom crescimento em 48h de incubação; o YNB recomendado por Ghannoum et al. fornece faixas de CIMs mais amplas; que o AM3 forneceu estreito intervalo de CIMs para a anfotericina B e que o YNB-2 utilizado nos testes com anfotericina B, evidenciou resultados muito semelhantes aos do caldo RPMI 1640.

This study compared the performance of three culture media used for the determination of minimal inhibitory concentration (MIC) of amphotericin B and fluconazole against 48 Cryptococcus neoformans strains. For amphotericin B the media employed were RPMI 1640 medium, as indicated by NCCLS as reference, Yeast Nitrogen Base (YNB) as proposed by Ghannoum et al. (YNB-1), Antibiotic medium 3 (AM3) indicated by others, and YNB as proposed by us (YNB-2). In YNB-1, amphotericin B showed broader MIC ranges, varying from 0.125 µg/ml to 1.0 µg/ml; in RPMI 1640 medium and YNB-2 the MICs were similar and in AM3 the MIC ranges were narrower. The same pattern was observed for MIC 90%. For fluconazole the media employed were RPMI 1640 and YNB-1. The MIC ranges were broader and elevated when YNB-1 was employed. MIC 50% were 2.0 µg/ml in RPMI 1640 medium and 4.0 µg/ml in YNB-1. Our results indicate that RPMI 1640 supplemented with 2% glucose allowed a good growth at 48h; YNB as recommended by Ghannoum et al. provided a broader MIC range, AM3 provided a narrow MIC range for amphotericin B, and YNB-2 was very similar to RPMI 1640 medium.