Abstract The viability of Lactobacillus bulgaricus in freeze-drying is of significant commercial interest to dairy industries. In the study, L.bulgaricus demonstrated a significantly improved (p < 0.05) survival rate during freeze-drying when subjected to a pre-stressed period under the conditions of 2% (w/v) NaCl for 2 h in the late growth phase. The main energy source for the life activity of lactic acid bacteria is related to the glycolytic pathway. To investigate the phenomenon of this stress-related viability improvement in L. bulgaricus, the activities and corresponding genes of key enzymes in glycolysis during 2% NaCl stress were studied. NaCl stress significantly enhanced (p < 0.05) glucose utilization. The activities of glycolytic enzymes (phosphofructokinase, pyruvate kinase, and lactate dehydrogenase) decreased during freeze-drying, and NaCl stress were found to improve activities of these enzymes before and after freeze-drying. However, a transcriptional analysis of the corresponding genes suggested that the effect of NaCl stress on the expression of the pfk2 gene was not obvious. The increased survival of freeze-dried cells of L. bulgaricus under NaCl stress might be due to changes in only the activity or translation level of these enzymes in different environmental conditions but have no relation to their mRNA transcription level.

O objetivo deste artigo é apresentar um novo modelo de relações topológicas fuzzy para objetos espaciais simples em Sistemas de Informações Geográficas (SIG). Aplica-se o conceito de espaço topológico fuzzy a objetos fuzzy simples para resolver as relações topológicas de modo mais eficiente e acurado. Inicialmente é proposta uma nova definição para segmentos de linha fuzzy e para regiões fuzzy com base na topologia computacional. Em seguida propõe-se um novo modelo para calcular as relações topológicas fuzzy entre os objetos espaciais. A análise do novo modelo aponta: (1) relações topológicas de dois objetos "crisp"; (2) relações topológicas entre um objeto "crisp" e um objeto fuzzy; (3) relações topológicas entre dois objetos "crisp". Finalmente, discutem-se alguns exemplos para demonstrar a validade do modelo, por meio de um experimento e comparações entre modelos existentes. É possível demonstrar que o método proposto pode realizar distinções mais precisas e á mais expressivo do que os modelos fuzzy existentes.

The goal of this paper is to present a new model of fuzzy topological relations for simple spatial objects in Geographic Information Sciences (GIS). The concept of computational fuzzy topological space is applied to simple fuzzy objects to efficiently and more accurately solve fuzzy topological relations, extending and improving upon previous research in this area. Firstly, we propose a new definition for simple fuzzy line segments and simple fuzzy regions based on computational fuzzy topology. And then, we also propose a new model to compute fuzzy topological relations between simple spatial objects, an analysis of the new model exposes:(1) the topological relations of two simple crisp objects; (2) the topological relations between one simple crisp object and one simple fuzzy object; (3) the topological relations between two simple fuzzy objects. In the end, we have discussed some examples to demonstrate the validity of the new model, through an experiment and comparisons of existing models, we showed that the proposed method can make finer distinctions, as it is more expressive than the existing fuzzy models.

Hyperthermia is one of the most effective adjuvant treatments for various cancers with few side effects. However, the underlying molecular mechanisms still are not known. N-myc downstream-regulated gene 2 (NDRG2), a tumor suppressor, has been shown to be involved in diverse cellular stresses including hypoxia, lipotoxicity, etc. In addition, Ndrg2 has been reported to be related to progression of gastric cancer. In the current study, our data showed that the apoptosis rate of MKN28 cells increased relatively rapidly to 13.4% by 24 h after treatment with hyperthermia (42°C for 1 h) compared to 5.1% in control cells (P < 0.05). Nevertheless, there was no obvious change in the expression level of total Ndrg2 during this process. Further investigation demonstrated that the relative phosphorylation levels of Ndrg2 at Ser332, Thr348 increased up to 3.2- and 1.9-fold (hyperthermia groupvs control group) at 3 h in MKN28 cells, respectively (P < 0.05). We also found that heat treatment significantly increased AKT phosphorylation. AKT inhibitor VIII (10 µM) decreased the phosphorylation level of Ndrg2 induced by hyperthermia. Accordingly, the apoptosis rate rose significantly in MKN28 cells (16.4%) treated with a combination of AKT inhibitor VIII and hyperthermia compared to that (6.8%) of cells treated with hyperthermia alone (P < 0.05). Taken together, these data demonstrated that Ndrg2 phosphorylation could be induced by hyperthermia in an AKT-dependent manner in gastric cancer cells. Furthermore, AKT inhibitor VIII suppressed Ndrg2 phosphorylation and rendered gastric cancer cells susceptible to apoptosis induced by hyperthermia.

Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were analyzed by suppression-subtractive hybridization. The birds were diagnosed as ML+ (or ML-) by specific ALV-J detection methods, involving serological tests for antigens and antibodies, and RT-PCR to detect viral RNA. A total of 59 partial gene sequences were revealed by differential screening of 496 forward and 384 reverse subtracted cDNA clones. Of these, 22 identified genes, including 8 up-regulated and 14 down-regulated, were related to immune functions, these genes being, MHC B-G antigen, translationally-controlled tumor protein (TPT1/TPTC), transferrin and ferritin, hemoglobin and Carbonic anhydrase. Four of the down-regulated genes were selected for further analysis, in view of their predicted roles in infection and immunity by real-time qRT-PCR, using RNA collected from the same birds as those used for SSH. The four genes were expressed at significantly lower levels (p < 0.001) in ALV-J infected birds than in non-infected ones.