Pesquisa | SciELO

Leachate from a composting plant was characterized and applied as a biofertilizer in lettuce crops. Characteristics of untreated (UE) and anaerobically treated (TE) leachate samples were compared. The pH of TE (8.2) increased in relation to the UE (5.2) due to an increase in ammonia nitrogen in TE (1197 mg L-1) compared to UE (859 mg L-1). Anaerobic treatment was efficient in the removal of organic matter from the leachate, evaluated by the decrease of dissolved organic carbon, biochemical oxygen demand, total organic carbon and total solids. K presented the highest concentration (1743 mg L-1) in TE, followed by Mg (135 mg L-1). Cd, Pb and Cr were present at low concentrations in the samples, 0.047, 0.206 and 0.081 mg L-1, respectively. Salmonella, thermotolerant coliforms and viable helminth eggs were not present in TE, which was applied as a biofertilizer in a lettuce crop and compared to mineral fertilization based on fresh matter and dry matter production. Lettuce production using TE was statistically equivalent to mineral fertilization. Toxic metals were not detected in the lettuce shoots. It was concluded that the anaerobically treated leachate from the composting company has the potential to be used as a biofertilizer.

https://doi.org/10.21577/0103-5053.20230085

Dicamba Degradation by Fenton-Like Process Using Iron/Biochar Composites
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Guimarães, Tiago

; Aguiar, Adalin C. M. de

; Silva, Elisa M. G. da

; Mielke, Kamila C.

; Costa, Marcelo M. da

; Silva, Antonio A. da

; Teixeira, Ana P C.

; Lopes, Renata P.

Journal of the Brazilian Chemical Society

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2023, Volume 34 Nº 12 Páginas 1753 - 1761

Resumo: En | Texto: En | PDF: En

In this work, an iron/biochar composite was evaluated as a catalyst for 3,6-dichloro-2-methoxybenzoic acid (dicamba) herbicide degradation by heterogeneous Fenton-like process. The biochar was produced from pyrolysis of coffee husks. After FeII adsorption by biochar, the material was submitted to chemical reduction, via borohydride, producing zero-valent iron nanoparticles (BIO-Fe0). The material was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy and energy dispersive X-ray spectroscopy, Raman scattering spectroscopy, nitrogen adsorption/desorption analysis, among other techniques. The BIO-Fe0 is a heterogeneous material, and the main constituent elements are carbon (C), oxygen (O), silicon (Si), sulfur (S) and iron (Fe). The dicamba degradation was monitored by high-performance liquid chromatography and biological assays using beans (Phaseolus vulgaris) as indicator species. The commercial dicamba (500 mL; 250 mg L 1) was submitted to Fenton-like process (initial pH 3.20; BIO-Fe0: 3 or 5 g; 1.00 mL H2O2 at 3.00 mmol L 1; 1.00 mL Al2(SO4)3 at 1.00 mol L 1; 25 ºC and 5 h of reaction), presenting ca. 100% of degradation. These treated solutions were used in a biological assay. The intoxication levels were less 30%. These results show that BIO-Fe0 has the potential to be used in the dicamba degradation by Fenton-like processes.

https://doi.org/10.21577/0103-5053.20230068

Response of plants used in cover crop mix to Meloidogyne enterolobii, Meloidogyne incognita and Pratylenchus brachyurus
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Lopes, Ana Paula Mendes

; Pereira, Alana Emanoele

; Baquião, Eunice Maria

; Amorin, Deucleiton Jardim

; Wilcken, Silvia Renata Siciliano

Pesquisa Agropecuária Tropical

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2023, Volume 53 elocation e75822

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Cover crop species have been used in mixes in order to improve soil conditions; however, it is important to evaluate the relationship of the employed cultivars with phytonematodes to identify those that do not multiply the pathogens in the cultivated area. This study aimed to assess the response of 11 plant species used in cover crop mix to Meloidogyne enterolobii, M. incognita and Pratylenchus brachyurus. Six experiments were conducted in a greenhouse, using a completely randomized design, with five replications. BMX Potência RR soybean and Santa Clara tomato were used as controls. In at least one of the experiments, IPR 91 Baili buckwheat was susceptible (reproduction factor - RF ≥ 1) to M. enterolobii, M. incognita and P. brachyurus. Common white lupin, URS Altiva white oat, Iapar 61 (Ibiporã) black oat and Embrapa 139 (Neblina) black oat were susceptible to M. enterolobii and M. incognita and resistant (RF < 1) and/or immune (RF = 0) to P. brachyurus. IPR 89 rye and IPR 116 radish were found susceptible to M. incognita and P. brachyurus and resistant to M. enterolobii. BRS 1501 millet and Embrapa 29 (Garoa) black oat were susceptible to M. incognita and resistant/immune to M. enterolobii and P. brachyurus. Brachiaria ruziziensis was considered resistant to M. enterolobii and M. incognita and susceptible to P. brachyurus. URS Corona white oat was the only cover crop considered to be resistant and/or immune to all the nematode species evaluated.

RESUMO Espécies de plantas de cobertura vêm sendo utilizadas em mixes, visando melhorar as condições do solo; porém, é importante avaliar a relação das cultivares utilizadas com fitonematoides, a fim de identificar aquelas que não multipliquem os patógenos na área cultivada. Objetivou-se avaliar a reação de 11 espécies vegetais utilizadas em mix de plantas de cobertura a Meloidogyne enterolobii, M. incognita e Pratylenchus brachyurus. Foram conduzidos 6 experimentos em casa-de-vegetação, em delineamento inteiramente casualizado, com 5 repetições, utilizando-se a soja BMX Potência RR e o tomate Santa Clara como testemunhas. Em pelo menos um dos experimentos, o trigo mourisco IPR 91 Baili foi suscetível (fator de reprodução - FR ≥ 1) a M. enterolobii, M. incognita e P. brachyurus. O tremoço-branco cv. comum, aveia branca URS Altiva, aveia preta Iapar 61 (Ibiporã) e aveia preta Embrapa 139 (Neblina) foram suscetíveis a M. enterolobii e M. incognita e resistentes (FR < 1) e/ou imunes (FR = 0) a P. brachyurus. O centeio IPR 89 e o nabo forrageiro IPR 116 foram suscetíveis a M. incognita e P. brachyurus e resistentes a M. enterolobii. O milheto BRS 1501 e a aveia preta Embrapa 29 (Garoa) foram suscetíveis a M. incognita e resistentes/imunes a M. enterolobii e P. brachyurus. A Brachiaria ruziziensis foi considerada resistente a M. enterolobii e M. incognita e suscetível a P. brachyurus. A aveia branca URS Corona foi a única planta considerada resistente e/ou imune para todas as espécies de nematoides avaliadas.

https://doi.org/10.1590/1983-40632023v5375822

Gathering patients and rheumatologists' perceptions to improve outcomes in idiopathic inflammatory myopathies
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Bertoglio, Isabela M.

; Abrahao, Glaucia F.

; de Souza, Fernando H.C.

; Miossi, Renata

; de Moraes, Paloma C.

; Shinjo, Samuel K.

; Bonfá, Eloisa

; Lopes, Michelle R. Ugolini

Clinics

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2022, Volume 77 elocation 100031

Resumo: En | Texto: En | PDF: En

Abstract Objective Therapeutic targets in Idiopathic Inflammatory Myopathies (IIM) are based on the opinions of physicians/specialists, which may not reflect the main concerns of patients. The authors, therefore, assessed the outcome concerns of patients with IIM and compared them with the concerns of rheumatologists in order to develop an IIM outcome standard set. Methods Ninety-three IIM patients, 51 rheumatologists, and one physiotherapist were invited to participate. An open questionnaire was initially applied. The top 10 answers were selected and applied in a multiple-choice questionnaire, inquiring about the top 3 major concerns. Answers were compared, and the agreement rate was calculated. Concerns were gathered in an IIM outcome standard set with validated measures. Results The top three outcome concerns raised by patients were medication side effects/muscle weakness/prevention functionality loss. The top three concerns among rheumatologists were to prevent loss of functionality/to ensure the quality of life/to achieve disease remission. Other's outcomes concerns only pointed out by patients were muscle pain/diffuse pain/skin lesions/fatigue. The agreement rate between both groups was 41%. Assessment of these parameters guided the development of an IIM standard set which included Myositis Disease Activity Assessment Visual Analogue Scale/Manual Muscle Testing/fatigue and pain Global Visual Analogue Scale/Health Assessment Questionnaire/level of physical activity. Conclusion The authors propose a novel standard set to be pursued in IIM routine follow-up, which includes not only the main patients/rheumatologist outcome concerns but also additional important outcomes only indicated by patients. Future studies are necessary to confirm if this comprehensive approach will result in improved adherence and ultimately in better assistance.

https://doi.org/10.1016/j.clinsp.2022.100031

Development and Validation of a Method for Ionic Dyes Determination in Guava Fibrous Matrix Using Reverse Phase Chromatography
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Reis, Marina F. dos

; Silva, Fabiano A

; Madureira, Fernando

; Vargas, Eugênia

; Oliveira, Lucas M. F

; Lopes, Renata P

Journal of the Brazilian Chemical Society

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Nov 2021, Volume 32 Nº 11 Páginas 2132 - 2139

Resumo: En | Texto: En | PDF: En

In this work, a method for food dyes determination in fibrous fruit pulps was developed. The analytes Tartrazine, Twilight Yellow FCF, Erythrosine, Orange G and Allura Red were analyzed by reverse phase chromatography with diode array (DAD) detection. The sample preparation consisted of a simple procedure, based on initial pH adjustment of the sample (pH = 10.0), followed by an extraction with acetonitrile (5.0 mL and agitation for 30 s), ultrasonic (5 min) and centrifugation (4000 rpm, 20 ºC, for 20 min). The supernatant had its pH adjusted to 3.0, followed by filtration. The method was validated, with the analytical curves prepared in a matrix, in the range of 50to150mg kg-1 for all the analytes studied (determination coefficients (R2) > 0.99). The method veracity was evaluated in terms of recovery, obtaining recoveries of 93-110%. The repeatability was less than 13.7%, while the intermediate precision was less than 19.0%. The limits of detection and quantification were determined, being 15 and 30 mg kg-1, respectively. The combined relative uncertainties were less than 13.8%. Therefore, it can be concluded that a simple method was developed, suitable for monitoring the presence of dyes in fruit pulps in routine analyzes.

https://doi.org/10.21577/0103-5053.20210105 15 downloads

Posicionamento Brasileiro sobre o Uso da Multimodalidade de Imagens na Cardio-Oncologia – 2021
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Melo, Marcelo Dantas Tavares de

; Paiva, Marcelo Goulart

; Santos, Maria Verônica Câmara

; Rochitte, Carlos Eduardo

; Moreira, Valéria de Melo

; Saleh, Mohamed Hassan

; Brandão, Simone Cristina Soares

; Gallafrio, Claudia Cosentino

; Goldwasser, Daniel

; Gripp, Eliza de Almeida

; Piveta, Rafael Bonafim

; Silva, Tonnison Oliveira

; Santo, Thais Harada Campos Espirito

; Ferreira, Waldinai Pereira

; Salemi, Vera Maria Cury

; Cauduro, Sanderson A.

; Barberato, Silvio Henrique

; Lopes, Heloísa M. Christovam

; Pena, José Luiz Barros

; Rached, Heron Rhydan Saad

; Miglioranza, Marcelo Haertel

; Pinheiro, Aurélio Carvalho

; Vrandecic, Bárbara Athayde Linhares Martins

; Cruz, Cecilia Beatriz Bittencourt Viana

; Nomura, César Higa

; Cerbino, Fernanda Mello Erthal

; Costa, Isabela Bispo Santos da Silva

; Coelho Filho, Otavio Rizzi

; Carneiro, Adriano Camargo de Castro

; Burgos, Ursula Maria Moreira Costa

; Fernandes, Juliano Lara

; Uellendahl, Marly

; Calado, Eveline Barros

; Senra, Tiago

; Assunção, Bruna Leal

; Freire, Claudia Maria Vilas

; Martins, Cristiane Nunes

; Sawamura, Karen Saori Shiraishi

; Brito, Márcio Miranda

; Jardim, Maria Fernanda Silva

; Bernardes, Renata Junqueira Moll

; Diógenes, Tereza Cristina

; Vieira, Lucas de Oliveira

; Mesquita, Claudio Tinoco

; Lopes, Rafael Willain

; Segundo Neto, Elry Medeiros Vieira

; Rigo, Letícia

; Marin, Valeska Leite Siqueira

; Santos, Marcelo José

; Grossman, Gabriel Blacher

; Quagliato, Priscila Cestari

; Alcantara, Monica Luiza de

; Teodoro, José Aldo Ribeiro

; Albricker, Ana Cristina Lopes

; Barros, Fanilda Souto

; Amaral, Salomon Israel do

; Porto, Carmen Lúcia Lascasas

; Barros, Marcio Vinícius Lins

; Santos, Simone Nascimento dos

; Cantisano, Armando Luís

; Petisco, Ana Cláudia Gomes Pereira

; Barbosa, José Eduardo Martins

; Veloso, Orlando Carlos Glória

; Spina, Salvador

; Pignatelli, Ricardo

; Hajjar, Ludhmilla Abrahão

; Kalil Filho, Roberto

; Lopes, Marcelo Antônio Cartaxo Queiroga

; Vieira, Marcelo Luiz Campos

; Almeida, André Luiz Cerqueira

Arquivos Brasileiros de Cardiologia

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Out 2021, Volume 117 Nº 4 Páginas 845 - 909

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20200266 103 downloads

METHOD DEVELOPMENT AND VALIDATION FOR THE FLUOROQUINOLONES DETERMINATION BY STABILIZED SILVER NANOPARTICLES
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Moraes, Luiza D. de M.

; Oliveira, André F. de

; Teixeira, Alvaro V. N. de C.

; Lopes, Renata P.

Química Nova

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Mar 2020, Volume 43 Nº 3 Páginas 271 - 278

Resumo: En | Texto: En | PDF: En

Silver nanoparticles (AgNPs) stabilized with glutathione (GSH) were used as nanosensors for the determination of fluoroquinolones such as ciprofloxacin (CIP), norfloxacin (NOR) and enrofloxacin (ENRO) in aqueous systems. The AgNPs were stabilized with GSH (Ag-GSH NPs), whereas the decrease of the surface plasmon resonance (SPR) band at 400 nm and the bathochromic shift of the absorption band were monitored. The method was validated and the analytical performance was verified. The results were satisfactory, with recoveries between 83.7 and 110% in almost all the experimental conditions and the repeatability and intermediate accuracy of less than 20%. The limits of detection were 0.397, 0.437 and 0.398 µmol L-1, and the limits of quantification were 1.203, 1.323 and 1.205 µmol L-1 for ciprofloxacin, norfloxacin and enrofloxacin, respectively. The method was selective in a group of drugs and applied in the determination of fluoroquinolones in potable water samples.

https://doi.org/10.21577/0100-4042.20170484 543 downloads

The Surface Treatment of Niobium Superconducting Reentrant Cavities by Means of High Temperature Nitrogen Plasma Based Ion Implantation
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Oliveira, Rogério Moraes

; Aguiar, Odylio Denys

; Araujo, Michel Felipe Lima de

; Silva, Matheus M. N. F.

; Mello, Carina B.

; Ferreira, Elvis

; Liccardo, Vincenzo

; Savonov, Graziela da Silva

; Baba, Koumei

; Souza, Renata Lopes Gonçalves de

Materials Research

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2019, Volume 22 Nº 6 elocation e20190277

Resumo: En | Texto: En | PDF: En

High Temperature Nitrogen Plasma Based Ion Implantation (HT-NPBII) has been used to treat the surface of niobium superconducting reentrant cavities, which are part of parametric transducers in a resonant-mass gravitational wave detector. The aim is to enhance the corresponding electrical quality factors (Q-factors) which are closely related with the increase of the sensitivity of the system. In this experiment, the cavities are immersed in plasma and bombarded by energetic nitrogen ions, which are implanted into the surfaces of these heated substrates. The heating temperature of the cavities is controlled during the treatment and its level directly affects the N implantation depth profile due to the diffusion process. Additional tailoring of the nitrogen doping can be performed by the adjustment of the intensity and the duty cycle of the high negative voltage pulses used for ion implantation. For implantations performed at 5 kV /20 µs /300 Hz/ 700 °C, nitrogen atoms occupy interstitial spaces in the crystal lattice of niobium. The treatment of niobium superconducting cavities under these parameters caused the enhancement of two orders of magnitude of respective Q-factors. A set of characterization techniques was performed herein in order to help with the understanding of the underlying mechanism behind this phenomenon.

https://doi.org/10.1590/1980-5373-mr-2019-0277 676 downloads

Brazilian recommendations on the safety and effectiveness of the yellow fever vaccination in patients with chronic immune-mediated inflammatory diseases
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Pileggi, Gecilmara Salviato

; Mota, Licia Maria Henrique Da

; Kakehasi, Adriana Maria

; Souza, Alexandre Wagner De

; Rocha, Aline

; Melo, Ana Karla Guedes de

; Fonte, Caroline Araujo M. da

; Bortoletto, Cecilia

; Brenol, Claiton Viegas

; Marques, Claudia Diniz Lopes

; Zaltman, Cyrla

; Borba, Eduardo Ferreira

; Reis, Enio Ribeiro

; Freire, Eutilia Andrade Medeiros

; Klumb, Evandro Mendes

; Christopoulos, Georges Basile

; Laurindo, Ieda Maria M.

; Ballalai, Isabella

; Costa, Izaias Pereira Da

; Michelin, Lessandra

; Valadares, Lilian David de Azevêdo

; Chebli, Liliana Andrade

; Lacerda, Marcus

; Toscano, Maria Amazile Ferreira

; Yazbek, Michel Alexandre

; Vieira, Rejane Maria R. De Abreu

; Magalhães, Renata

; Kfouri, Renato

; Richtmann, Rosana

; Merenlender, Selma Da Costa Silva

; Valim, Valeria

; Assis, Marcos Renato De

; Kowalski, Sergio Candido

; Trevisani, Virginia Fernandes Moça

Advances in Rheumatology

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2019, Volume 59 elocation 17

Resumo: En | Texto: En | PDF: En

Abstract Background: In Brazil, we are facing an alarming epidemic scenario of Yellow fever (YF), which is reaching the most populous areas of the country in unvaccinated people. Vaccination is the only effective tool to prevent YF. In special situations, such as patients with chronic immune-mediated inflammatory diseases (CIMID), undergoing immunosuppressive therapy, as a higher risk of severe adverse events may occur, assessment of the risk-benefit ratio of the yellow fever vaccine (YFV) should be performed on an individual level. Main body of the abstract: Faced with the scarcity of specific orientation on YFV for this special group of patients, the Brazilian Rheumatology Society (BRS) endorsed a project aiming the development of individualized YFV recommendations for patients with CIMID, guided by questions addressed by both medical professionals and patients, followed an internationally validated methodology (GIN-McMaster Guideline Development). Firstly, a systematic review was carried out and an expert panel formed to take part of the decision process, comprising BRS clinical practitioners, as well as individuals from the Brazilian Dermatology Society (BDS), Brazilian Inflammatory Bowel Diseases Study Group (GEDIIB), and specialists on infectious diseases and vaccination (from Tropical Medicine, Infectious Diseases and Immunizations National Societies); in addition, two representatives of patient groups were included as members of the panel. When the quality of the evidence was low or there was a lack of evidence to determine the recommendations, the decisions were based on the expert opinion panel and a Delphi approach was performed. A recommendation was accepted upon achieving ≥80% agreement among the panel, including the patient representatives. As a result, eight recommendations were developed regarding the safety of YFV in patients with CIMID, considering the immunosuppression degree conferred by the treatment used. It was not possible to establish recommendations on the effectiveness of YFV in these patients as there is no consistent evidence to support these recommendations. Conclusion: This paper approaches a real need, assessed by clinicians and patient care groups, to address specific questions on the management of YFV in patients with CIMID living or traveling to YF endemic areas, involving specialists from many areas together with patients, and might have global applicability, contributing to and supporting vaccination practices. We recommended a shared decision-making approach on taking or not the YFV.

https://doi.org/10.1186/s42358-019-0056-x 1111 downloads

10.

Removal of Beta-Lactams Antibiotics through Zero-Valent Copper Nanoparticles
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Oliveira, Lucas M. F.

; Nascimento, Mayra A.

; Guimarães, Yuri M.

; Oliveira, André F.

; Silva, Antônio A.

; Lopes, Renata P.

Journal of the Brazilian Chemical Society

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Ago 2018, Volume 29 Nº 8 Páginas 1630 - 1637

Resumo: En | Texto: En | PDF: En

The removal of the beta-lactam antibiotics (ceftriaxone and cefadroxil) through zero-valent copper nanoparticle (nZVC) was studied in this work. Excellent removal degrees (> 85%) were obtained for both analytes in only 20 min of reaction. Studies were performed in both oxic and anoxic conditions, and in the presence of t-butyl alcohol (TBA), an inhibitor of radicals. The results did not show significant changes. Therefore, the hydroxyl radicals are not the main species responsible for the removal. Total organic carbon cefadroxil analysis indicated a removal of 57% after 180 min of reaction. Studies involving Cu+ indicated that probably these are the principal species responsible for the removal of antibiotics. Kinetic studies have shown that two-phase reaction occurred in the antibiotics removal process and both phases followed pseudo-first order kinetic model. The first mechanism is related to the antibiotics degradation by Cu+ species and the second mechanism is related to the antibiotics adsorption by hydroxides/oxides of Cu2+ species.

https://doi.org/10.21577/0103-5053.20180034 1008 downloads

11.

Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
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Dantas, Alisson F.

; Lopes, Renata M.

; Fascineli, Maria L.

; José, Solange C.B.R.

; Pádua, Juliano G.

; Gimenes, Marcos A.

; Grisolia, Cesar K.

Genetics and Molecular Biology

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Mar 2018, Volume 41 Nº 1 Páginas 145 - 153

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects at all the concentrations in the evaluated accessions of rice and beans, except in one accession of beans at the lowest concentration (5 mg/L). Both species showed sensitivity to MMS. The comet test can be proposed for the measurement of genomic instability in accessions of rice and beans in gene banks, as being more sensitive than the cytogenetic tests used.

https://doi.org/10.1590/1678-4685-gmb-2017-0020 1037 downloads

12.

TEMPERATURE, LIGHT, AND DESICCATION TOLERANCE IN SEED GERMINATION OF Mauritia flexuosa L.F.
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Almeida, Lívia Caroline Praseres de

; Pivetta, Kathia Fernandes Lopes

; Gimenes, Renata

; Romani, Gustavo de Nobrega

; Ferraz, Marcos Vieira

; Mazzini-Guedes, Renata Bachin

Revista Árvore

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2018, Volume 42 Nº 3 elocation e420305

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO As palmeiras são amplamente utilizadas como plantas ornamentais, além da importância para a sustentabilidade e para a indústria. A propagação comercial destas plantas é realizada por meio de sementes. Devido à importância da espécie e à necessidade de elucidar alguns aspectos relacionados à germinação das sementes, este trabalho teve como objetivo verificar o efeito de temperatura, regimes de luz e tolerância a dessecação na germinação de sementes da palmeira Mauritia flexuosa L.f. Foram realizados dois experimentos e em ambos o delineamento experimental foi o inteiramente casualizado. No primeiro, estudou-se o efeito da temperatura e da luz e os tratamentos foram dispostos em esquema fatorial 6 x 2 (seis temperaturas: 20 ºC, 25 ºC, 30 ºC, 35 ºC, 20-30 ºC e 25-35 ºC combinadas com presença e ausência de luz); foram quatro repetições de 25 sementes por parcela. No segundo, estudou-se a tolerância a dessecação e os tratamentos foram cinco teores de água nas sementes (51%, 48%, 45%, 30% e 26%) aferidos a cada três dias de um lote mantido em condições ambiente; foram quatro repetições de 25 sementes por parcela. Avaliou-se a porcentagem de germinação e Índice de Velocidade de Germinação. Os dados foram submetidos à análise de variância; as médias de temperatura e de luz foram comparadas pelo teste de Tukey (P < 0.05) e para dessecação realizou-se a análise de regressão polinomial. A temperatura de 20-30 ºC foi a que apresentou maiores médias de porcentagem e de velocidade de germinação independentemente da presença ou ausência de luz. As sementes de M. flexuosa se mostraram sensíveis à dessecação.

ABSTRACT Palm trees are widely used as ornamental plants, besides its sustainability and industry aspects. These plants are commercially propagated via seeds. Due to its importance and the need for seed germination information, this study aimed to verify the effect of temperature, light regimes, and desiccation tolerance on germination of Mauritia flexuosa L.f. For that, two completely randomized experiments were carried out. The first consisted of treatments arranged in a 6 x 2 factorial scheme, testing six temperatures (20, 25, 30, 35, 20-30, and 25-35 ºC) and light presence and absence. The second comprised five seed water contents (51, 48, 45, 30, and 26%) for desiccation tolerance evaluations. Water content measurements were made every three days, from a sample lot maintained at room temperature. In both trials, each treatment was composed of four replicates with 25 seeds per plot. Germination rate (GR) and germination speed index (GSI) were calculated, and the data were analyzed by analysis of variance (ANOVA). Temperature and light means were compared by the Tukey’s test (p < 0.05), and the desiccation ones by a polynomial regression. The highest GR was observed at 20-30 ºC, regardless of light presence. Moreover, M. flexuosa seeds were sensitive to desiccation.

https://doi.org/10.1590/1806-90882018000300005 958 downloads

13.

Synthesis by Click Reactions and Antiplasmodial Activity of Lupeol 1,2,3-Triazole Derivatives
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Borgati, Tatiane F.

; Pereira, Guilherme R.

; Brandão, Geraldo C.

; Santos, Juliana O.

; Fernandes, Dayane Aparecida M.

; Paula, Renata C. de

; Nascimento, Maria Fernanda A. do

; Soares, Luciana F.

; Lopes, Júlio César D.

; Souza Filho, José D. de

; Oliveira, Alaíde B. de

Journal of the Brazilian Chemical Society

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Out 2017, Volume 28 Nº 10 Páginas 1850 - 1856

Resumo: En | Texto: En | PDF: En

Lupeol, a triterpene frequently found in Asteraceae plant species, showed moderate to low activity in different strains of Plasmodium falciparum, the most virulent malaria etiological agents. In this work, lupeol was isolated from Parahancornia fasciculata, a plant that is used to treat malaria in the Amazonia region. In the search of more activity lupeol derivatives, five new 1,2,3-triazole hybrid molecules were synthetized by copper-catalyzed azide-alkyne cycloaddition. The antiplasmodial activity of the semi-synthetic compounds were evaluated by the lactate dehydrogenase assay; the lupeol propargyl ether was the only one to disclosing increased activity (half maximal inhibitory concentration-IC50-62.0 ± 1.92 µmol L-1) in relation to lupeol (IC50 117.00 µmol L-1). Therefore, this work revealed a new class of interesting lupeol derivatives that can be obtained by linking electron donors to the hydroxy group at C-3.

https://doi.org/10.21577/0103-5053.20170013 899 downloads

14.

Trichilia catigua: therapeutic and cosmetic values
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Longhini, Renata

; Lonni, Audrey A.S.G.

; Sereia, Ana Luiza

; Krzyzaniak, Letícia M.

; Lopes, Gisely C.

; Mello, João Carlos P. de

Revista Brasileira de Farmacognosia

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Abr 2017, Volume 27 Nº 2 Páginas 254 - 271

Resumo: En | Texto: En | PDF: En

ABSTRACT Medicinal plants play an important role in human health care. It is estimated that about 25–30% of all drugs are evaluated as therapeutic agents derived from natural products. Research in the pharmaceutical industry has demonstrated that for complex diseases, natural products still represent a valuable source for the production of new chemical compounds, since they possess privileged structures. Among Brazilian biodiversity, "catuaba" is popularly used as a tonic to treat fatigue, stress, impotence, memory deficits, and digestive disorders. Studies show antibacterial, trypanocidal, antioxidant, antiarrhythmic, antidepressant, improvement of memory, anti-inflammatory and antinociceptive activities, as well as phytocosmetic activity in cellulite treatment and in anti-ageing. The Brazilian plants known and used as catuaba are represented by more than twenty different species; however, the plant most commonly found in Brazil as "catuaba" is the species Trichilia catigua A. Juss., Meliaceae. Thus, the aim of this paper is to present a review of T. catigua, with emphasis on biological activities, chemical and analytical development and formulations in order to provide a broader and deeper insight, seeking a herbal medicine and/or phytocosmetic as well as future prospects for commercial exploitation and directions for future studies.

https://doi.org/10.1016/j.bjp.2016.10.005 3694 downloads

15.

Molecular diversity of human parvovirus B19 during two outbreaks of erythema infectiosum in Brazil
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Garcia, Rita de Cássia Nasser Cubel

; Pereira, Renata Freire Alves

; Azevedo, Kátia Martins Lopes de

; Castro, Tatiana Xavier de

; Mello, Francisco C.A.

; Setubal, Sérgio

; Siqueira, Marilda M.

; Brown, David

; Oliveira, Solange Artimos de

Brazilian Journal of Infectious Diseases

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Fev 2017, Volume 21 Nº 1 Páginas 102 - 106

Resumo: En | Texto: En | PDF: En

Abstract This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996–2006, a period with two distinct outbreaks of B19V infection: 1999–2000 and 2004–2005. A total of 27 sera from patients with erythema infectiosum and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b). The phylogenetic tree supported the division of the G1a into two well-defined clades with 1.3% of divergence. The low diversity of the G1a strains may be explained by the fact that all patients had acute B19V infection and 30/32 sera were collected during two distinct outbreaks. The G3b strain was from an HIV-infected patient who seroconverted to anti-B19 IgG antibodies in September/2005. This is the first report of G3b in the state of Rio de Janeiro.

https://doi.org/10.1016/j.bjid.2016.11.002 791 downloads

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