Abstract Background: Different immune mechanisms of myocardial damage involved in the pathophysiology of Chagas disease coexist with high titers of autoantibodies induced by T. cruzi . There are few studies in the literature about the adaptive role of anti-β1 and anti-M2 antibodies in chronic Chagas cardiomyopathy (CCC). Objectives: To evaluate the association between anti-β1 and anti-M2 antibodies with heart rate variability (HRV) parameters on 24h Holter monitoring and the rate-pressure product (RPP) on cardiopulmonary exercise testing (CPET). Methods: Anti-β1 and anti-M2 antibody titers were measured by enzyme-linked immunosorbent assay (ELISA) in 64 patients affected by CCC. Analysis of HRV was performed through the time-domain indices NNs, mean NN, SDNN, SDANN, SDNN index, NNNs, RMSSD, and pNN50. Spearman’s correlation coefficient was used to assess the association between antibody titers and numerical variables. The Mann–Whitney test was used for comparison between two groups. Multiple linear regression was used to identify independent variables capable of explaining anti-β1 and anti-M2 antibody titers at the 5% significance level. Results: On 24h Holter, during the period of greatest parasympathetic activation (2:00-6:00 a.m.), an inverse association was found between anti-β1 titers and SDNN (rs=-0.13, p =0.041, n=43), as well as a direct association between anti-M2 titers and SDANN ( r s=0.317, p =0.039, n=43). Regarding CPET variables, anti-β1 titers were directly associated with RPP (rs=0.371, p =0.005, n=56). The subgroup of patients with a normal chronotropic response showed higher anti-β1 titers than the subgroup with an impaired response (p=0.023). RPP was an independent explanatory variable for anti-β1 titers, although with a low coefficient of determination (R2=0.147). Conclusion: The findings of this study suggest that, in patients with CCC, anti-β1 and anti-M2 antibodies may affect HRV parameters. RPP was directly associated with higher anti-β1 titers.

ABSTRACT. This study aimed to evaluate the effect of cultivating a combination of common vetch (Vicia sativa L.) with black oats (Avena strigosa Schreb.) on the chemical composition of forage and the grazing behavior of heifers. To accomplish this, two paddocks 2500m2 each from a Voisin Rational Grazing management system were divided into three blocks each and then into thirds (278m²) characterizing a randomized block design. Three different forage compositions were distributed into these thirds: oats grown alone, vetch grown alone, and oats grown with vetch. Forage samples were collected after 65 days through the square method. Right after collection, three groups of four heifers each grazed the plots for two hours in a 3x3 double Latin Square design for behavioral observation, grazing simulation through the hand-plucking method, and biting rate determination. Forage samples collected either by hand-plucking or the square method, were analyzed for chemical composition and “in vitro” degradability. Statistical analyses were performed using the R package lme4. Data were evaluated with linear mixed-effects models. The inclusion of common vetch significantly increased forage production and oat protein content, but decreased the fiber content, which promoted better “in vitro” degradability. Grazing frequency was higher in pasture where oats were grown with vetch, but the biting rate was similar in all the three forage compositions evaluated. Forage collected by the square method did not differ from forage consumed by the heifers, probably meaning low herbage selectivity by heifers. Furthermore, no interaction of investigated variables occurred between forage compositions and the method of collection. The inclusion of common vetch with black oats increased forage chemical composition, “in vitro” degradability, and forage production, thus having positive effects on the time cows spent grazing.

Hemorrhagic transformation is a complex phenomenon where brain tissue bleeds, which could be associated or not to an increase in the neurological deficit after the acute ischemic stroke. The aim of our study was to evaluate clinical predictors of hemorrhagic transformation in patients with non-lacunar ischemic stroke. We performed a prospective analysis of the clinical records and images of patients with non-lacunar ischemic stroke. Demographics, vascular risk factors, previous medications and the information of the event in patients with and without hemorrhagic transformation were here compared. We included in this study 747 patients with non-lacunar stroke, the mean age was 77 ± 11 years and 61% were females. In the univariate analysis, the age, a history of hypertension, atrial fibrillation, chronic kidney disease and the previous use of oral anticoagulation resulted statistically significant. In the multivariate analysis of logistic regression adjusted by age and vascular risk factors: the age > 80 years (OR 3.6, CI 95% 1.8-7.6), the pulse pressure > 60 mmHg at admission (OR 5.3, CI 95% 3.2-9.1), the chronic kidney disease (OR 3, CI 95% 2.5-3.8) and the presence of previous atrial fibrillation (OR 3.5, CI 95% 2.1-6.1) were associated with and increased risk of hemorrhagic transformation. The predictors of hemorrhagic transformation in our cohort showed a relationship with severe vascular illness. The identification of these patients could influence therapeutic decisions that could increase the risk of hemorrhagic transformation.

La transformación hemorrágica es un fenómeno complejo en el que el tejido cerebral isquémico sangra; este proceso puede asociarse o no a un deterioro del estado neurológico inicial. El objetivo de nuestro estudio fue evaluar los predictores clínicos de trasformación hemorrágica en los pacientes con accidente cerebrovascular isquémico no lacunar. Se analizaron las historias clínicas y las imágenes de pacientes con infarto cerebral no lacunar incluidos prospectivamente en un programa de prevención secundaria. Se compararon datos demográficos, factores de riesgo vascular, medicación previa y datos del evento de los pacientes con transformación hemorrágica y sin ella. Se incluyeron 747 pacientes, la edad promedio fue de 77 ± 11 años, 61% mujeres. En el análisis univariado, la edad, el antecedente de hipertensión arterial, la fibrilación auricular, la insuficiencia renal crónica y la anticoagulación oral previa se relacionaron significativamente con la presencia de transformación hemorrágica. En el análisis multivariado de regresión logística ajustado por edad y factores de riesgo vascular, resultaron predictores de transformación hemorrágica: la edad > 80 años (OR 3.6; IC 95% 1.8-7.6), la presión de pulso > 60 mmHg al ingreso (OR 5.3; IC 95% 3.2-9.1), la insuficiencia renal crónica (OR 3; IC 95% 2.5-3.8) y el antecedente de fibrilación auricular (OR 3.5; IC 95% 2.1-6.1). En nuestra cohorte los predictores clínicos de conversión hemorrágica del infarto cerebral muestran una relación con la gravedad de la enfermedad vascular. La identificación de estos pacientes influenciaría en la toma de decisiones terapéuticas que pudieran incrementar el riesgo de transformación hemorrágica.

This study investigated the rate of human papillomavirus (HPV) persistence, associated risk factors, and predictors of cytological alteration outcomes in a cohort of human immunodeficiency virus-infected pregnant women over an 18-month period. HPV was typed through L1 gene sequencing in cervical smears collected during gestation and at 12 months after delivery. Outcomes were defined as nonpersistence (clearance of the HPV in the 2nd sample), re-infection (detection of different types of HPV in the 2 samples), and type-specific HPV persistence (the same HPV type found in both samples). An unfavourable cytological outcome was considered when the second exam showed progression to squamous intraepithelial lesion or high squamous intraepithelial lesion. Ninety patients were studied. HPV DNA persistence occurred in 50% of the cases composed of type-specific persistence (30%) or re-infection (20%). A low CD4+T-cell count at entry was a risk factor for type-specific, re-infection, or HPV DNA persistence. The odds ratio (OR) was almost three times higher in the type-specific group when compared with the re-infection group (OR = 2.8; 95% confidence interval: 0.43-22.79). Our findings show that bonafide (type-specific) HPV persistence is a stronger predictor for the development of cytological abnormalities, highlighting the need for HPV typing as opposed to HPV DNA testing in the clinical setting.

INTRODUÇÃO: O conhecimento da marcha nos portadores de Doença Pulmonar Obstrutiva Crônica (DPOC) e a identificação de variáveis podem promover o desenvolvimento de estratégias de reabilitação, com vista à manutenção e preservação da autonomia. OBJETIVO: Avaliar o padrão de marcha do portador de DPOC, a partir da análise bidimensional com o software Simi Motion®. MÉTODO: Delineamento transversal, estudo de casos, com seis portadores de DPOC, sexo masculino, 64,00 ± 8,07 anos, IMC 22,28 ± 2,46 kg/ m2, volume expiratório forçado no primeiro segundo (VEF1) 35,17 ± 25,79% predito, capacidade vital forçada (CVF) 64,83 ± 17,84% predito. Para dados cinemáticos utilizou-se videografia bidimensional, Simi Motion®, acoplado à câmera de vídeo digital. Registros em 60 frames por segundo e tempo de aquisição de ciclo de marcha. RESULTADOS: Os dados cinemáticos encontraram-se variados; cada indivíduo apresentou peculiaridades sobrepostas aos padrões básicos. Observou-se forte correlação negativa entre cadência e PImax (p = 0,002 e r = - 0,96), entre comprimento de passo e PEmax (p = 0,007 e r = - 0,93). Para a qualidade de vida (SGQR), associações diretas, qualidade de vida total (QVT) e percentual da fase de apoio (p = 0,086 e r = 0,75); qualidade de vida impacto (QVI) e percentual da fase de apoio (p = 0,09 e r = 0,74). CONCLUSÃO: Avaliar a marcha do portador de DPOC possibilitou inferir objetivamente, delineando intervenções terapêuticas. Os benefícios esperados da pesquisa, tanto para o indivíduo quanto para a sociedade, é uma nova maneira de avaliar e incrementar a marcha, elemento fundamental para manutenção da independência funcional e qualidade de vida.

INTRODUCTION: Knowledge about gait functional in COPD patients and the identification of the associated variables in these characteristics can support the development of specific approaches in rehabilitation with the objective to maintain the autonomy. OBJECTIVE: To evaluate the gait pattern adopted by COPD patients using a bidimensional analysis. METHOD: Transversal delineation, study of cases, carried through with six patients of COPD, the masculine sex, 64,00 ± 8,07 years of age, index of corporal mass (IMC) 22,28 ± 2,46 kg/m2, forced expiratory volume in the first second (VEF1) 35.17 ± 25.79% predicted, vital capacity forced (CVF) 64.83 ± 17.84% predicted. For kinematic data, we used bidimensional videography Simi Motion, connected was used the camera of digital video. The registers in 60 frames for second and time of acquisition of march cycle. RESULTS: The kinematic data had met varied, since each individual presented overlapping peculiarities the basic standards. One strong negative correlation between cadence and PImax (p = 0,002 and r = - 0,96), between length of step and PEmax was observed (p = 0,007 and r = - 0,93). For the quality of life (SGQR), direct associations had been found, quality of total life (QVT) and percentage of the support phase (p = 0,086 and r = 0,75), and also quality of life impact (QVI) and percentage of the support phase (p = 0,09 and r = 0,74). CONCLUSION: To evaluate the standard of march the DPOC carrier, made possible in them to infer objective on this, possible to delineate therapeutically interventions. The benefits waited with such research, as much for the individual as for the society, it was in a new way to evaluate and to develop the march, basic element for maintenance of functional independence and quality of life.

The greenhouse whitefly, Trialeurodes vaporariorum Westwood, is the most common and abundant whitefly in Argentine horticultural greenhouse crops, especially in tomato (Solanum lycopersicum). Resistance in some wild tomato relatives, such as S. peruvianum, S. habrochaites and S. pennellii to the greenhouse whitefly has been described. The Mi gene confers effective resistance against several species of insects, among them the sweet potato whitefly, Bemisia tabaci Gennadius. Resistance to T. vaporariorum was found in the prebreeding line FCN 93-6-2, derived from a cross between S. lycopersicum cultivar Uco Plata INTA (MiMi) and the wild line FCN 3-5 S. habrochaites. The purpose of this study was to evaluate resistance to T. vaporariorum in tomato genotypes and to study the relationship between this resistance and the presence of the REX-1 marker, which is linked to the Mi gene. In a free-choice assay, the average number of adults per leaf and the number of immatures on the middle and basal plant parts were analyzed. In a no-choice assay, the oviposition rate and adult survival rate were calculated. For all variables analyzed, FCN 3-5 was the most resistant strain. Variations were found in the F2 progeny between the prebreeding line FCN 13-1-6-1 and cv. Uco Plata INTA. Results from the F2 progeny indicate that resistance to T. vaporariorum may be polygenic with transgressive segregation. Whitefly resistance was found to be independent of the REX-1 marker.