Pesquisa | SciELO

Pesticides have been pointed out as hormone disruptors and may significantly affect the prognosis of hormone-dependent diseases such as breast cancer (BC). Here, we investigated the impact of occupational pesticide exposure on systemic cortisol levels in female rural workers diagnosed with BC. Occupational exposure was assessed by interviews with a standardized questionnaire. Plasma samples (112 from pesticide-exposed women and 77 from unexposed women) were collected in the afternoon, outside the physiological cortisol peak, and analyzed by a chemiluminescent paramagnetic immunoassay for the quantitative determination of cortisol levels in serum and plasma. The results from both groups were categorized according to patients' clinicopathological and exposure data. BC pesticide-exposed women presented higher levels of cortisol than the unexposed. Higher cortisol levels were also detected in the exposed group with more aggressive disease (triple-negative BC), with tumors over 2 cm, with lymph node metastases, and with high risk of disease recurrence and death. These findings demonstrated that there is an association between pesticide exposure and BC that affected cortisol levels and correlated to poor disease prognosis.

https://doi.org/10.1590/1414-431x2023e13060

Nutritional composition of human milk and its association with maternal and perinatal factors
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Marano, Daniele

; Melo, Raquel Ximenes

; Silva, Danielle Aparecida da

; Vilarim, Marina Machado

; Moreira, Maria Elisabeth Lopes

Revista Paulista de Pediatria

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2024, Volume 42 elocation e2023001

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: The objective of this study was to evaluate the influence of maternal and perinatal factors on the nutritional composition of human milk. Methods: A cross-sectional study was conducted between November 2018 and January 2020, with 181 donors selected in Tertiary Health Units of the Unified Health System — from one collection station and five Human Milk Banks. Data were collected through a standardized questionnaire. To be fit to be a donor in a Human Milk Banks and produce mature milk were the eligibility criteria to participate in the study. We excluded milk samples with Dornic acidity above 8° D. The dependent variables were the macronutrients of human milk (i.e., carbohydrates, proteins, lipids, and total energy), that were analyzed using spectroscopy with the Miris Human Milk Analyzer™. The maternal and perinatal factors were the independent variables. Results: Women with pre-gestational obesity and gestational weight gain above the recommendation showed a lower protein concentration compared to eutrophic women (median=0.8, interquartile range (IQR): 0.7–0.9 vs. median=0.8, and IQR: 0.8–1.0) and those with adequate gestational weight gain (median=0.8, IQR: 0.7–0.9 vs. median=0.9, and IQR: 0.8–1.0), respectively. The other analyzed factors (i.e., maternal habits, comorbidities, and perinatal factors) were not associated with the nutritional composition of human milk. Conclusions: The assessment of factors associated with the nutritional composition of human milk is extremely important to assist postpartum care. Pre-gestational obesity and inadequate gestational weight gain were the only factors statistically associated with the nutritional composition of human milk as they impacted its protein content.

RESUMO Objetivo: Avaliar a influência de fatores maternos e perinatais na composição nutricional do leite humano. Métodos: Estudo transversal, realizado entre novembro de 2018 e janeiro de 2020, com 181 doadoras selecionadas em Unidades Terciárias de Saúde do Sistema Único de Saúde de um Posto de Coleta e cinco Bancos de Leite Humano. Foram coletados dados das variáveis maternas e dos recém-nascidos por meio de questionário padronizado. Estar apta para ser doadora em um Banco de Leite Humano e produzir leite maduro foram os critérios de elegibilidade para participar do estudo. As amostras de leite com acidez Dornic acima de 8° D foram excluídas. Os macronutrientes do leite humano, variáveis dependentes (carboidratos, proteínas, lipídeos, energia total) foram analisados pela técnica de espectroscopia de transmissão no infravermelho médio com o Miris Human Milk Analyzer™. Os fatores maternos e perinatais foram as variáveis independentes. Resultados: Mulheres com obesidade pré-gestacional e ganho de peso gestacional acima do recomendado apresentaram menor concentração proteica em relação às eutróficas (mediana=0,8, intervalo interquartil [IQR]: 0,7–0,9 vs. mediana=0,8, IQR: 0,8–1,0) e aquelas com ganho de peso gestacional adequado (mediana=0,8, IQR: 0,7–0,9 vs. mediana=0,9, IQR: 0,8–1,0), respectivamente. Os demais fatores analisados (hábitos maternos, comorbidades, fatores perinatais) não se associaram com a composição nutricional do leite humano. Conclusões: A avaliação dos fatores associados à composição nutricional do leite humano é de extrema importância para auxiliar os cuidados pós-parto. A obesidade pré-gestacional e o ganho de peso gestacional inadequado foram os únicos fatores estatisticamente associados com a composição nutricional do leite humano por terem impactado o seu teor de proteína.

https://doi.org/10.1590/1984-0462/2024/42/2023001

Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Tobias-Machado, Marcos

; Ornellas, Antonio A.

; Hidaka, Alexandre K.

; Medina, Luis G.

; Mattos, Pablo A. L.

; Besio, Ruben S.

; Abreu, Diego

; Castro, Pedro R.

; Nishimoto, Ricardo H.

; Astigueta, Juan

; Dourado, Aurus

; Machado, Roberto D.

; Magnabosco, Wesley J.

; Corona-Montes, Victor

; Villoldo, Gustavo M.

; Zampolli, Hamilton C.

; Taha, Anis

; Auad, Pericles R.

; Faria, Eliney F.

; Arantes, Paulo B. O.

; Tavares, Alessandro

; Nascimento, Francisco S. M. S.

; Brazão Jr., Eder S.

; Rocha, Maurício M.

; Costa, Walter H.

; Panico, Vinicius

; Reis, Leonardo O.

; Almeida-Carrera, Roberto J.

; Silva, Rafael C.

; Zequi, Stênio C.

; Calixto, José R. R.

; Sotelo, Rene

International braz j urol

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Out 2023, Volume 49 Nº 5 Páginas 580 - 589

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

Aberraciones corneales en pacientes con indicación de cirugía refractiva con láser de excímeros
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics

Machado Fernández, Enrique José

; Orta Hernández, Eduardo Adrián

; Xie, Qin

; Guerra Almaguer, Michel

; Cárdenas Díaz, Taimi

Revista Cubana de Oftalmología

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jun 2023, Volume 36 Nº 2 elocation e1763

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Objective: To determine corneal aberrations in patients indicated for excimer laser refractive surgery. Methods: A retrospective, cross-sectional and descriptive study was carried out with 161 eyes of 81 adult patients of both sexes with indication of excimer laser surgery for the correction of their refractive defect. Age, sex, defocus, astigmatism, coma, trefoil and spherical aberration were defined as variables, determined by routine topographic study with the KeratronTM Scout topographer, Optikon. Results: The following average values were obtained: defocus of -4.17 ± 0.29 D (-16.15 to 8.5 D) and absolute of 4.94 ± 0.199 (10 to 16.5 D), astigmatism of -1.56 ± 0.09 D (-9.44 to -0.09 D), coma of 0.25 ± 0.016 (0.01 to 1.5 D), trefoil of 0.204 ± 0.016 (0.01 to 1.18 D) and spherical aberration of 0.316 ± 0.018 D (0.0 to 1.27D). In 75 % of the cases, the absolute values for defocus were lower than 6.56; for astigmatism, lower than 0.33 D; for coma, lower than 0.33; for trefoil, lower than 0.25; and for spherical aberrations, lower than 0.32 D. Conclusions: The average values of corneal aberrations for defocus, astigmatism, coma, trefoil and spherical aberration are in the range of values reported in the scientific literature, while the distribution of corneal aberrations values present displacement of most of the cases towards the lower values of the determination range.

RESUMEN Objetivo: Determinar las aberraciones corneales en pacientes con indicación de cirugía refractiva con láser de excímeros. Métodos: Se realizó un estudio retrospectivo, transversal, descriptivo, con 161 ojos de 81 pacientes adultos, de ambos sexos con indicación de cirugía con láser de excímeros para la corrección de su defecto refractivo. Se definieron como variables edad, sexo, desenfoque, astigmatismo, coma, trefoil y aberración esférica, las que se determinaron por el estudio topográfico de rutina con el topógrafo KeratronTM Scout, Optikon. Resultados: Se obtuvieron los siguientes valores promedios: desenfoque -4,17 ± 0,29 D (-16,15 a 8,5 D) y absoluto 4,94 ± 0,199 (10 a 16,5 D), astigmatismo -1,56 ± 0,09 D (-9,44 a -0,09 D), coma 0,25 ± 0,016 (0,01 a 1,5 D), trefoil 0,204 ± 0,016 (0,01 a 1,18 D) y aberración esférica 0,316 ± 0,018 D (0,0 a 1,27D). En el 75 % de los casos los valores absolutos de desenfoque fueron inferiores a 6,56, de astigmatismo inferior a 0,33 D, de coma menor que 0,33, trefoil inferior a 0,25 y aberraciones esféricas menores que 0,32 D. Conclusiones: Los valores promedio de las aberraciones corneales desenfoque, astigmatismo, coma, trefoil y aberración esférica se encuentran en el rango de los valores reportados en la literatura científica y la distribución de los valores de las aberraciones corneales presentan desplazamiento de la mayoría de los casos hacia los valores más bajo del rango de determinación.

Pre-slaughter management in Northeast Brazil and the effects on thermophysiological indicators in pigs and pH45
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Melo, Kilvia K. de S. V.

; Machado, Nítalo A. F.

; Barbosa Filho, José A. D.

; Peixoto, Maria S. M.

; Andrade, Ana P. C. de

; Costa, Jennifer A. da

; Oliveira, Ana B. A.

; Sales, João J. de M.

Revista Brasileira de Engenharia Agrícola e Ambiental

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Abr 2023, Volume 27 Nº 4 Páginas 287 - 292

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Práticas inadequadas de manejo antes do abate podem comprometer o bem-estar animal e a qualidade da carne suína. Este estudo visou avaliar os efeitos do período de transporte (TT), período de descarga (TD), e período de pré-abate (TE) nos indicadores termofisiológicos animais e no pH da carne suína, utilizando uma abordagem multivariada. Este estudo foi realizado num matadouro localizado em Aquiraz, Ceará, Brasil. Um total de 60 suínos mestiços com 107 ± 5 kg de peso corporal foram distribuídos em três grupos experimentais: grupo A (TT = 180 min, TD ≤ 10 min, e TE = 24 horas), grupo B (TT = 60 min, TD ≤ 15 min, e TE = 18 horas), e grupo C (TT = 45 min, TD ≤ 5 min, e TE = 24 horas). A análise discriminante canônica foi implementada e as diferenças entre tratamentos são representadas graficamente. Os dois primeiros componentes foram responsáveis por 97% dos tratamentos. A análise discriminante mostrou que o grupo C apresentou diferenças multivariadas em relação aos outros, particularmente com temperatura retal elevada antes do abate (38,4 ± 0,23 °C) e frequência respiratória (131 ± 7,0 respirações min-1) e temperatura retal (39,7 ± 0,18 °C) à chegada ao matadouro. O período de transporte (TT), período de descarga (TD) e o stress térmico das instalações influenciaram variáveis fisiológicas, tais como a temperatura retal e a frequência respiratória. Além disso, as variáveis de estresse fisiológico também favoreceram cortes com pH básico, resultando em perda de qualidade.

ABSTRACT Inadequate pre-slaughter handling practices may compromise animal welfare and pork quality. This study aimed to evaluate the effects of transport period (TT), unloading period (UT), and pre-slaughtering period (WT) on animal thermophysiological indicators and pork pH using a multivariate approach. This study was conducted in a slaughterhouse located in Aquiraz, Ceará state, Brazil. A total of 60 mestizo pigs with 107 ± 5 kg body weight were distributed into three experimental groups: group A (TT = 180 min, UT ≤ 10 min, and WT = 24 hours), group B (TT = 60 min, UT ≤ 15 min, and WT = 18 hours), and group C (TT = 45 min, UT ≤ 5 min, and WT = 24 hours). Canonical discriminant analysis was implemented and the differences between treatments are represented graphically. The first two components accounted for 97% treatments. The discriminant analysis showed that group C presented multivariate differences in relation to others, particularly with high rectal temperature before slaughter (38.4 ± 0.23 °C) and respiratory rate (131 ± 7.0 breaths min-1) and rectal temperature (39.7 ± 0.18 °C) upon arrival at the slaughterhouse. Transport period (TT), unloading period (UT), and thermal stress of the facilities influenced physiological variables, such as rectal temperature and respiratory rate. Furthermore, the physiological stress variables also favored cuts with basic pH, resulting in quality loss.

https://doi.org/10.1590/1807-1929/agriambi.v27n4p287-292

Phenolic Compounds from Leaves of Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae): A Brazilian Atlantic Forest Tree
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Tiepo, Angelica N.

; Coutinho, Isabel D.

; Machado, Guilherme O.

; Oliveira, Halley C.

; Pimenta, José Antonio

; Henning, Liliane Marcia M.

; Colnago, Luiz Alberto

; Stolf-Moreira, Renata

Journal of the Brazilian Chemical Society

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

Jan 2023, Volume 34 Nº 1 Páginas 146 - 152

Resumo: En | Texto: En | PDF: En

Cariniana estrellensis (Raddi) Kuntze seedlings have been used in forest restoration programs in the Atlantic Forest biome. However, despite the economic and ecological relevance of this species, to date no general method for rapid identification of primary and secondary metabolites of C. estrellensis leaves has been reported. In this work, we explore the feasibility of using rapid analysis by nuclear magnetic resonance techniques and liquid chromatography coupled to diode array, charged aerosol, and mass detection. The main secondary metabolites identified were hydrolysable tannins, quinic acid, hydroxycinnamic acids (trans-p-coumaric acid, cis p coumaric acid, and trans-ferulic acid), and flavonoids (kaempferol and quercetin derivatives). These compounds are particularly useful as chemotaxonomic markers for the genus Cariniana and for the family Lecythidaceae, and may also be important for pharmacological uses and for the survival of the seedlings in reforestation programs.

https://doi.org/10.21577/0103-5053.20220083

Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Souza, K. M.

; Mendes, I. C.

; Dall’Igna, D. M.

; Repolês, B. M.

; Resende, B. C.

; Moreira, R. S.

; Miletti, L. C.

; Machado, C. R.

; Vogel, C. I. G.

Brazilian Journal of Biology

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 83 elocation e243910

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O reparo por excisão de nucleotídeos (NER) atua reparando danos no DNA, como lesões causadas por cisplatina. A proteína Xeroderma Pigmentosum complementation group C (XPC) está envolvida no reconhecimento de danos pela via de reparação global do genoma pelo NER (GG-NER) e tem sido estudada em diferentes organismos devido à sua importância em outros processos celulares. Neste trabalho, estudamos proteínas do NER em Trypanosoma cruzi e Trypanosoma evansi, parasitos de humanos e animais, respectivamente. Modelos tridimensionais das proteínas XPC de T. cruzi e T. evansi foram feitos e observou-se poucas diferenças estruturais entre estas proteínas. Durante testes, a inserção do gene XPC de T. evansi (TevXPC) em T. cruzi resultou em crescimento celular mais lento em condições normais. Após o tratamento com cisplatina, T. cruzi superexpressando seu próprio gene XPC (TcXPC) foi capaz de recuperar as taxas de divisão celular mais rapidamente do que T. cruzi expressando o gene TevXPC. Com base nesses testes, sugere-se que TevXPC (sendo uma proteína exógena em T. cruzi) interfere negativamente nos processos celulares em que TcXPC (a proteína endógena) está envolvida. Isso provavelmente ocorreu pois TevXPC é capaz de interagir com algumas moléculas ou proteínas endógenas de T.cruzi, mas é incapaz de interagir com outras. Isso reforça a importância do correto funcionamento de XPC dentro da célula.

Abstract Nucleotide excision repair (NER) acts repairing damages in DNA, such as lesions caused by cisplatin. Xeroderma Pigmentosum complementation group C (XPC) protein is involved in recognition of global genome DNA damages during NER (GG-NER) and it has been studied in different organisms due to its importance in other cellular processes. In this work, we studied NER proteins in Trypanosoma cruzi and Trypanosoma evansi, parasites of humans and animals respectively. We performed three-dimensional models of XPC proteins from T. cruzi and T. evansi and observed few structural differences between these proteins. In our tests, insertion of XPC gene from T. evansi (TevXPC) in T. cruzi resulted in slower cell growth under normal conditions. After cisplatin treatment, T. cruzi overexpressing its own XPC gene (TcXPC) was able to recover cell division rates faster than T. cruzi expressing TevXPC gene. Based on these tests, it is suggested that TevXPC (being an exogenous protein in T. cruzi) interferes negatively in cellular processes where TcXPC (the endogenous protein) is involved. This probably occurred due interaction of TevXPC with some endogenous molecules or proteins from T.cruzi but incapacity of interaction with others. This reinforces the importance of correctly XPC functioning within the cell.

https://doi.org/10.1590/1519-6984.243910 462 downloads

How many species of Mollusca are there in Brazil? A collective taxonomic effort to reveal this still unknown diversity
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Machado, Fabrizio M.

; Miranda, Marcel S.

; Salvador, Rodrigo B.

; Pimenta, Alexandre D.

; Côrtes, Mariana O.

; Gomes, Jaime A.J.

; Miyahira, Igor C.

; Agudo-Padrón, Ignacio

; Oliveira, Cléo D.C.

; Caetano, Carlos H.S.

; Coelho, Paulo R.S.

; D’Ávila, Sthefane

; Arruda, Eliane P. de

; Almeida, Sérgio M. de

; Gomes, Suzete R.

; Alvim, Juliana

; Galvão-Filho, Hilton

; L. Ferreira-Júnior, Augusto

; Marques, Rodrigo C.

; Martins, Inês

; Souza, Leonardo S. de

; Arruda, Janine O.

; Cavallari, Daniel C.

; Santos, Sonia B. dos

; Pedro, Natan C.

; Salles, Anna C. de A.

; Dornellas, Ana P.S.

; Lima, Tarcilla C. de

; Amaral, Vanessa S. do

; Silva, Fernanda S.

; Passos, Flávio D.

; Thiengo, Silvana S.

; Leite, Tatiana S.

; Simone, Luiz R.L.

Zoologia (Curitiba)

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 40 elocation e23026

Resumo: En | Texto: En | PDF: En

ABSTRACT The expression ‘you need to know to conserve’ is a well-known cliche among biologists. Documenting the richness of a group of organisms is the first step towards understanding biodiversity and preparing efficient conservation plans. In this context, many efforts have been made to quantify the number of species on Earth and estimate the number of species still unknown to science. A few countries have complete and integrated databases estimating the approximate number of species recorded for their territory, particularly in the Global South. In Brazil, a country of continental dimensions, revealing the richness of the second most diverse clade of invertebrates (=Mollusca) has been a goal of taxonomists. Recently, in an unprecedented, collective, and integrated effort among Brazilian malacologists, it was possible to estimate how many valid species of molluscs are there in Brazil. In this effort, more than 30 mollusc experts joined together to update the Taxonomic Catalogue of the Brazilian Fauna (TCBF), a governmental website that allows a quick and real-time updating of all Metazoan. So far, more than 5,000 updates have been made in TCBF, indicating the presence of 3,552 valid species of molluscs in Brazil, distributed among the main clades as follows: Caudofoveata (10 spp.), Solenogastres (6 spp.), Polyplacophora (35 spp.), Scaphopoda (43 spp.), Cephalopoda (92 spp.), Bivalvia (629 spp.) and Gastropoda (2,737 spp.). The present study, in addition to demonstrating for the first time the richness of Brazilian molluscs, also presents the state of the art of this important phylum of invertebrates highlighting its most representative and neglected groups.

https://doi.org/10.1590/s1984-4689.v40.e23026

10.

Molecular insight for baru Dipteryx alata (Fabaceae) populations based on novel SSRs
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Silva, Rayleen Whaiti Lopes da

; Machado, Sarah Silva

; Faria, Karina de Cassia

; Oliveira, Fernanda Ancelmo de

; Souza, Anete Pereira de

; Menezes, Ivandilson Pessoa Pinto de

; Silva, Joaquim Manoel da

Acta Botanica Brasilica

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 37 elocation e20220168

Resumo: En | Texto: En | PDF: En

ABSTRACT Baru tree (Dipteryx alata) is an arboreal, fruitful plant native to the Cerrado biome with an important socioeconomic impact. Populations of this species are a good model to study anthropogenic disturbances on the biome through the genetic information. In this study, we developed seven new polymorphic microsatellite markers for D. alata, using an enriched genomic library. We performed loci characterization in three populations, obtaining a total of 49 alleles, with an average of 5 to 5.57 alleles per locus. A significant content of polymorphic information was obtained, as indicated by the average expected heterozygosity (uHE), with a total average of 0.58 to 0.65 per locus. The average value of the observed heterozygosity (Ho) was also high, with a total average of 0.73 to 0.85 per locus. Some of the loci are in linkage disequilibrium, such as DalatG6 with DalatB3, DalatH3 and DalatB4. The estimate of the combined loci for the probability of paternity exclusion obtained an average value of 1.00 for all loci, and the average combined probability of identity, the values were (1.210^-5) to (4.410^-6). All markers are informative and suitable for studies on genetic diversity and population structure, aiming at the conservation and management of the species.

https://doi.org/10.1590/1677-941x-abb-2022-0168

11.

Molecular detection of hemoplasmas in rescued black-eared opossums (Didelphis aurita Wied-Neuwied, 1826) from southeastern Brazil, with evidence of a novel genotype infecting marsupials
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Oliveira, Ágatha Ferreira Xavier de

; Calchi, Ana Cláudia

; Mongruel, Anna Claudia Baumel

; Stocco, Anieli Vidal

; Stocco, Naiara Vidal

; Costa, Alexandre Carvalho

; Mureb, Elizabeth Neves

; Pires, Jeferson Rocha

; Guimarães, Andresa

; Balthazar, Daniel de Almeida

; Machado, Rosangela Zacarias

; André, Marcos Rogério

; Baldani, Cristiane Divan

Revista Brasileira de Parasitologia Veterinária

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 32 Nº 2 elocation e016422

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract There is a growing concern about the participation of wild hosts and reservoirs in the epidemiology of several pathogens, particularly within the context of environmental changes and the expansion of the One Health concept. The aim of this study was to investigate the presence of hemoplasmas in opossums rescued from the metropolitan region of Rio de Janeiro state, Brazil. Blood samples were collected from 15 Didelphis aurita and subjected to DNA extraction and PCR using primers for the 16S rRNA and 23S rRNA genes. Physical examination and hematological analysis were also performed. Three out of 15 opossums tested positive for hemotropic Mycoplasma spp. by PCR and showed hematological alterations such as anemia and leukocytosis. Clinical signs were non-specific and associated to traumatic lesions. The phylogenetic analysis indicated that the hemoplasma detected was positioned between 'Ca. Mycoplasma haemodidelphis' detected in D. virginiana from North American and hemoplasmas recently detected in D. aurita from the state of Minas Gerais, Brazil. This study indicates the existence of hemoplasma infections in D. aurita from the metropolitan region of Rio de Janeiro, and reinforce the need for new epidemiological inquiries to clarify the participation of these in the dynamics of circulation of tick-borne pathogens.

Resumo Há uma crescente preocupação com a participação de hospedeiros e reservatórios silvestres na epidemiologia de diversos patógenos, principalmente no contexto das mudanças ambientais e da expansão do conceito "One Health". O objetivo deste estudo foi investigar a presença de hemoplasmas em gambás resgatados da região metropolitana do estado do Rio de Janeiro, Brasil. Amostras de sangue foram coletadas de 15 Didelphis aurita e submetidas à extração de DNA e PCR utilizando-se "primers" para os genes 16S rRNA e 23S rRNA. O exame físico e a análise hematológica também foram realizados. Três dos 15 gambás testaram positivo para Mycoplasma spp. hemotrópico por PCR. Os sinais clínicos eram inespecíficos e associados a lesões traumáticas. Anemia e leucocitose foram detectadas em animais positivos. A análise filogenética indicou que o hemoplasma detectado estava posicionado entre 'Ca. Mycoplasma haemodidelphis' detectado em D. virginiana da América do Norte e hemoplasmas recentemente detectados em D. aurita do estado de Minas Gerais, Brasil. Este estudo indica a existência de infecções por hemoplasmas em D. aurita, da região metropolitana do Rio de Janeiro, e reforça a necessidade de novos inquéritos epidemiológicos, para esclarecer a participação destes na dinâmica de circulação de patógenos transmitidos por carrapatos.

https://doi.org/10.1590/s1984-29612023015

12.

Brain-derived neurotrophic factor serum levels following ketamine and esketamine intervention for treatment-resistant depression: secondary analysis from a randomized trial
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Caliman-Fontes, Ana Teresa

; Leal, Gustavo C.

; Correia-Melo, Fernanda S.

; Paixao, Camilla S.

; Carvalho, Michelle S.

; Jesus-Nunes, Ana Paula

; Vieira, Flavia

; Magnavita, Guilherme

; Bandeira, Igor D.

; Mello, Rodrigo P.

; Beanes, Graziele

; Silva, Samantha S.

; Echegaray, Mariana

; Carvalho, Lucas P.

; Machado, Paulo

; Sampaio, Aline S.

; Cardoso, Taiane de A.

; Kapczinski, Flávio

; Lacerda, Acioly L. T.

; Quarantini, Lucas C.

Trends in Psychiatry and Psychotherapy

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 45 elocation e20210298

Resumo: En | Texto: En | PDF: En

Abstract Objectives Evidence suggests that ketamine’s influence on brain-derived neurotrophic factor (BDNF) might be involved in its mechanism of rapid antidepressant action. We aimed to evaluate the differential impact of ketamine and esketamine on serum BDNF levels and its association with response patterns in treatment-resistant depression (TRD). Methods Participants (n = 53) are from a randomized, double-blind clinical trial comparing the efficacy of single-dose ketamine (0.5mg/kg, n = 27) and esketamine (0.25mg/kg, n = 26) in TRD. Depression severity was assessed before and 24 hours, 72 hours, and 7 days after the intervention, using the Montgomery-Åsberg Depression Rating Scale (MADRS). Blood samples were collected before infusion, 24 hours, and 7 days afterwards. Results There were no significant changes in BDNF levels at post-infusion evaluation points, and no difference in BDNF levels comparing ketamine and esketamine. Both drugs exhibited similar therapeutic effect. There was no association between BDNF levels and response to treatment or severity of depressive symptoms. Conclusion There was no significant treatment impact on BDNF serum levels – neither with ketamine nor esketamine – despite therapeutic response. These results suggest that ketamine or esketamine intervention for TRD has no impact on BDNF levels measured at 24 hours and 7 days after the infusion. This clinical trial is registered on the Japan Primary Registries Network: UMIN000032355.

https://doi.org/10.47626/2237-6089-2021-0298

13.

Avaliação no Mundo Real de um Stent Eluidor de Sirolimus de Hastes Ultrafinas em Pacientes com Infarto do Miocárdio com Supradesnivelamento do Segmento ST Submetidos à Intervenção Coronária Percutânea Primária (Registro INSTEMI)
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Araujo, Gustavo N.

; Machado, Guilherme P.

; Moura, Marcia

; Silveira, Anderson D.

; Bergoli, Luiz Carlos

; Fuchs, Felipe Costa

; Gonçalves, Sandro Cadaval

; Wainstein, Rodrigo Vugman

; Lemos, Pedro A.

; Quadros, Alexandre S.

; Wainstein, Marco V.

Arquivos Brasileiros de Cardiologia

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 120 Nº 6 elocation e20220594

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background The current gold standard of coronary drug-eluting stents (DES) consists of metal alloys with thinner struts and bioresorbable polymers. Objectives Our aim was to compare an ultrathin strut, sirolimus-eluting stent (Inspiron®) with other third-generation DES platforms in patients with ST-elevation myocardial infarction (STEMI) submitted to primary percutaneous coronary intervention (PCI). Methods We analyzed data from a STEMI multicenter registry from reference centers in the South Region of Brazil. All patients were submitted to primary PCI, either with Inspiron® or other second- or third-generation DES. Propensity score matching (PSM) was computed to generate similar groups (Inspiron® versus other stents) in relation to clinical and procedural characteristics. All hypothesis tests had a two-sided significance level of 0.05. Results From January 2017 to January 2021, 1711 patients underwent primary PCI, and 1417 patients met our entry criteria (709 patients in the Inspiron® group and 708 patients in the other second- or third-generation DES group). After PSM, the study sample was comprised of 706 patients (353 patients in the Inspiron® group and 353 patients in the other the other second- or third-generation DES group). The rates of target vessel revascularization (OR 0.52, CI 0.21 – 1.34, p = 0.173), stent thrombosis (OR 1.00, CI 0.29 – 3.48, p = 1.000), mortality (HR 0.724, CI 0.41 – 1.27, p = 0.257), and major cardiovascular outcomes (OR 1.170, CI 0.77 – 1.77, p = 0.526) were similar between groups after a median follow-up of 17 months. Conclusion Our findings show that Inspiron® was effective and safe when compared to other second- or third-generation DES in a contemporary cohort of real-world STEMI patients submitted to primary PCI.

Resumo Fundamento O padrão-ouro atual dos stents farmacológicos (SF) coronários consiste em ligas metálicas com hastes mais finas e polímeros bioabsorvíveis. Objetivos Nosso objetivo foi comparar um stent eluidor de sirolimus de hastes ultrafinas (Inspiron®) com outras plataformas de SF de terceira geração em pacientes com infarto do miocárdio com supradesnivelamento do segmento ST (IAMCSST) submetidos à intervenção coronária percutânea (ICP) primária. Métodos Analisamos dados de um registro multicêntrico de IAMCSST de centros de referência da Região Sul do Brasil. Todos os pacientes foram submetidos à ICP primária, seja com Inspiron® ou outro SF de segunda ou terceira geração. Foi calculado pareamento por escore de propensão (PEP) para gerar grupos semelhantes (Inspiron® versus outros stents) em relação às características clínicas e do procedimento. Todos os testes de hipótese tiveram um nível de significância bilateral de 0,05. Resultados De janeiro de 2017 a janeiro de 2021, 1.711 pacientes foram submetidos à ICP primária, e 1.417 pacientes preencheram nossos critérios de inclusão (709 pacientes no grupo Inspiron® e 708 pacientes no grupo dos outros SF de segunda ou terceira geração). Após PEP, a amostra do estudo foi composta por 706 pacientes (353 pacientes no grupo Inspiron® e 353 pacientes no grupo dos demais SF de segunda ou terceira geração). As taxas de revascularização do vaso alvo (odds ratio [OR] 0,52; intervalo de confiança [IC] 0,21 a 1,34; p = 0,173), trombose de stent (OR 1,00; IC 0,29 a 3,48;p = 1,000), mortalidade (hazard ratio 0,724; IC 0,41 a 1,27; p = 0,257) e os desfechos cardiovasculares maiores (OR 1,170; IC 0,77 a 1,77; p = 0,526) foram semelhantes entre os grupos após um acompanhamento mediano de 17 meses. Conclusão Nossos achados mostram que o stent Inspiron® foi eficaz e seguro quando comparado a outros SF de segunda ou terceira geração em uma coorte contemporânea do mundo real de pacientes com IAMCSST submetidos à ICP primária.

https://doi.org/10.36660/abc.20220594

14.

Antifungal potential of essential oils from Pectis brevipedunculata and Dizygostemon riparius in anthracnose control in mango
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Corrêa, Lukas Allayn Diniz

; Rodrigues, Antônia Alice Costa

; Dias, Larisse Raquel Carvalho

; Silva, Erlen Keila Candido e

; Monteiro, Odair dos Santos

; Oliveira, Leonardo de Jesus Machado Gois de

Revista Brasileira de Fruticultura

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 45 elocation e-889

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Essential oils have been widely studied because they are natural sources of substances that have antimicrobial properties. In fruit growing, especially in mango crop, anthracnose (Colletotrichum gloeosporioides) is considered the main disease because it causes series of damages in the fruit production chain and, in this sense, essential oils can be an option in this disease control. The objective of this study was to evaluate the effect of essential oils obtained from the leaves of Chá-de-moça (Pectis brevipedunculata) and Melosa (Dizygostemon riparius) in the in vitro and in vivo control of the C. gloeosporioides fungus, in seedlings and mango fruits. The in vitro bioassays were performed by testing the effect of different concentrations of essential oils (0, 1, 2, 3 and 4 µl/mL) on the reduction of mycelial growth of the C. gloeosporioides fungus. Additionally, the anthracnose control in mango seedlings (Tommy Atkins, Constantina, Comum and Rosa cultivars) was evaluated by foliar application of essential oils at 4 µl/mL concentration. Disease control in fruits of the same mango cultivars was evaluated at 3 µl/mL concentration of essential oils. The evaluation was carried out by measuring the average diameter of the colonies, for the in vitro treatment, and the lesions for the in vivo treatments, in two diametrically opposite directions. There was a decrease in the fungus mycelial growth in all tested concentrations. There was a decrease in the disease severity from the sixth day after the oils application in Tommy Atkins cultivar seedlings, when treated with both essential oils at 4 µl/mL concentration. As for preventive treatments with fruits, it was observed that all cultivars achieved a reduction in severity from 54.83% at 3 µl/mL concentration with the use of both essential oils. Given the results obtained, it was observed that the essential oils P. brevipedunculata and D. riparius can be a viable alternative in the anthracnose control in mango culture.

Resumo Os óleos essenciais têm sido largamente estudados por serem fontes naturais de substâncias que possuem propriedades antimicrobianas. Na fruticultura, especialmente na cultura de manga, a antracnose (Colletotrichum gloeosporioides) é consideradaa principal doença por causar uma série de prejuízos na cadeia produtiva dos frutos, e,nesse sentido,os óleos essenciais podem ser uma opção no controle desta doença. O objetivodeste trabalho foi avaliar o efeito dos óleos essenciais obtidos das folhas de Chá-de-moça (Pectis brevipedunculata) e Melosa (Dizygostemon riparius) no controle in vitro e in vivo do fungo C. gloeosporioides, em mudas e frutos de mangueira. Os bioensaios in vitro foram realizados testando-se o efeito de diferentes concentrações dos óleos essenciais (0; 1;2; 3 e 4 µl/mL) sob a redução do crescimento micelial do fungo C. gloeosporioides.Adicionalmente, avaliou-se o controle da antracnose em mudas de manga (cultivares Tommy Atkins,Constantina, Comum e Rosa), pela aplicação foliar dos óleos essenciais, na concentração de 4 µl/mL. O controle da doença em frutos das mesmas cultivares de manga foi avaliado naconcentração de 3 µl/mL dos óleos essenciais. A avaliação foi feita pela mensuração dodiâmetro médio das colônias, para o tratamento in vitro, e das lesões, para ostratamentos in vivo, em dois sentidos diametralmente opostos. Houve diminuição do crescimento micelial do fungo em todas as concentrações testadas. Observou-se diminuição na severidade dadoença a partir do sexto dia após aplicação dos óleos nas mudas de cultivar Tommy, quandotratada com ambos os óleos essenciais, na concentração de 4 µl/mL. Já para os tratamentospreventivos com frutos, foi observado que todas as cultivares obtiveram redução daseveridade a partir de 54,83%, na concentração de 3 µl/mL, com uso de ambos os óleos essenciais.Diante dos resultados obtidos, foi observado que os óleos essenciais P. brevipedunculata e D. ripariuspodem ser uma alternativa viável no controle de antracnose na cultura de manga.

https://doi.org/10.1590/0100-29452023889

15.

Biotechnological and socio-environmental potential of Campomanesia adamantium (Myrtaceae): an interdisciplinary review socioenvironmental socio environmental Myrtaceae (Myrtaceae) (Myrtaceae
Facebook Twitter

Outras redes sociais
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Outras redes

Métricas
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Zorgetto-Pinheiro, V. A.

; Kuff, H. M.

; Machado, G. T.

; Guimarães, R. C. A.

; Pott, A.

; Campos, R. P.

; Nascimento, V. A.

; Bogo, D.

Brazilian Journal of Biology

Métricas do periódico
- Sobre o periódico
- SciELO Analytics

2023, Volume 83 elocation e273473

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Guavira (Campomanesia adamantium, Myrtaceae) é um fruto nativo do Cerrado brasileiro e de importância sócio econômica para a população indígena e tradicional do Centro-Oeste. Esta é uma revisão bibliográfica das propriedades da guavira e seus derivados e, após a discussão de estudos experimentais, uma abordagem interdisciplinar é conduzida destacando a importância dos Sistemas Agroflorestais como uma ferramenta de restauração ecológica para alavancar a cadeia produtiva da guavira enquanto fornece serviços ecossistêmicos. Muitos grupos de pesquisa estudaram os efeitos dos polifenóis e outros compostos bioativos e propriedades biológicas desta fruta e de outras partes da planta, como antibióticos, antioxidantes, anti-inflamatórios, anti-hiperlipidêmicos, antidiarreicos e antitumorais, proteção cardiovascular e hepática e ação contra a dor neuropática. Além disso, os subprodutos da guavira beneficiam a saúde intestinal das aves, da mesma forma que os antibióticos adicionados à sua alimentação. Foram, também, encontrados diversos produtos biotecnológicos, como farinha de polpa, tempero da casca, protetor solar e óleo de semente semelhante ao azeite com potencial farmacêutico e industrial. Concluímos enfatizando a importância da guavira para a restauração e preservação do ameaçado Cerrado brasileiro e para o desenvolvimento socioambiental da agricultura familiar. A mesma abordagem e estudo são bem-vindos e necessários em outras regiões e domínios em todo o mundo, tendo sua flora nativa como meio para um fim restaurador. Campomanesia adamantium Myrtaceae CentroOeste. CentroOeste Centro Oeste. Oeste Centro-Oeste experimentais ecossistêmicos planta antioxidantes antiinflamatórios, antiinflamatórios anti inflamatórios, inflamatórios anti-inflamatórios antihiperlipidêmicos, antihiperlipidêmicos hiperlipidêmicos, hiperlipidêmicos anti-hiperlipidêmicos antitumorais neuropática disso aves alimentação Foram também biotecnológicos polpa casca industrial familiar bemvindos bem vindos mundo restaurador

Abstract Guavira (Campomanesia adamantium, Myrtaceae) is a native fruit from the Brazilian Cerrado savanna and is socio-economically important for the indigenous and traditional people living in the Central-West. This is a bibliographic review of the biological properties of guavira and its derivatives, and, after discussing experimental studies, an interdisciplinary approach is conducted highlighting the im-portance of Agroforestry Systems as an ecological restoration tool to leverage the production chain of guavira while providing ecosystem services. Many research groups studied effects of polyphenols and other bioactive compounds and biological properties of this fruit and other plant parts such as antibiotic, antioxidant, anti-inflammatory, anti-hyperlipidemic, anti-diarrheic and antitumoral activities, cardiovascular and hepatic protection and action against neuropathic pain. Besides, guavira by-products benefit poultry intestinal health, similarly to antibiotics added to their feed. Furthermore, several biotechnological products were found, like pulp flour, seasoning from the peel, sunscreen, and seed oil similar to olive oil with pharmaceutical and industrial potential. We conclude by emphasizing the importance of guavira for restoration and preservation of the threatened Brazilian Cerrado, and for the socio-environmental development of family agriculture. The same approach and study are welcome and necessary in other regions and domains worldwide having their native flora as means for a restorative end. Campomanesia adamantium Myrtaceae socioeconomically socio economically CentralWest. CentralWest Central West. West Central-West derivatives studies im portance services antibiotic antioxidant antiinflammatory, antiinflammatory anti inflammatory, inflammatory anti-inflammatory antihyperlipidemic, antihyperlipidemic hyperlipidemic, hyperlipidemic anti-hyperlipidemic antidiarrheic diarrheic activities pain Besides byproducts health feed Furthermore found flour peel sunscreen potential socioenvironmental environmental agriculture end

https://doi.org/10.1590/1519-6984.273473

Exibindo

itens por página

Página de 21 Próxima

Selecione um ou mais resultados

Todos Ordem alfabética

Resultados

Gráfico comparativo Download CSV

Visualizar estatísticas de

Enviar resultado

E-mail do(s) destinatários*

Utilize ; (ponto e vírgula) para mais de um e-mail.

Enviar por e-mail

Esta página

Registros selecionados

Todos os registros (max. 300)

Alterar remetente, assunto e comentários

Seu email

Assunto

Comentários

Alterar remetente, assunto e comentários

Exportar resultados

Formato de exportação:

RIS (para EndNote, ProCite, Reference Manager, RefWorks, Citavi, etc.)

BibTeX

Citação

CSV (para Excel, etc)

Exportar:

Esta página

Itens selecionados

Todos os registros (max. 2000)

Mostrar mais

Sem resultados

Não foram encontrados documentos para sua pesquisa

Glossário e ajuda para busca

Você pode enriquecer sua busca de uma forma muito simples. Use os índices de pesquisa combinados com os conectores (AND ou OR) e especifique cada vez mais sua busca.

Por exemplo, se você deseja buscar artigos sobre
casos de dengue no Brasil em 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
Email: scielo@scielo.org

Todo o conteúdo de www.scielo.org, exceto onde está identificado, está licenciado sob uma Licença Creative Commons.
saiba mais »