Resumo: Introdução: Os limites da carga horária (CH) de saúde coletiva (SC) antes do internato (AI) não estão definidos nas Diretrizes Curriculares Nacionais, nem em outros documentos, e não foram investigados de forma abrangente em estudos nacionais. Objetivo: Analisar a CH de SC AI em escolas médicas (EM) brasileiras. Método: Estudo transversal, com escolas reconhecidas pelo Ministério da Educação que iniciaram as atividades até 31 de dezembro de 2017. Os sites das 323 escolas existentes foram consultados, e foram incluídas aquelas que disponibilizavam seus currículos na internet com detalhamento de CH de SC AI. As variáveis foram região geográfica e administração da escola, CH do curso de medicina e CH de SC AI, incluindo também as CH de epidemiologia, bioestatística e saúde do trabalhador. Os dados foram analisados usando-se estatística descritiva, teste de qui-quadrado (Ӽ2) de Pearson para variáveis categóricas e testes Mann-Whitney-U (U) e Ӽ2 de Kruskal-Wallis para variáveis contínuas. Resultados: Foram incluídas 222 das 323 EM existentes (68,7%), sendo 83 gratuitas (37,4%) e 139 não gratuitas (62,6%). A mediana da CH total de SC AI foi de 440,0 horas (P25-75 = 300,0 - 640,0), equivalente a 5,4% (P25-75 = 3,5 - 7,8) da CH total do curso. A mediana da CH de SC AI em horas das escolas privadas e municipais foi de, respectivamente, 480,0 (P25-75 = 330,7 - 679,2) e 576,0 (P25-75 = 360,0 - 766,0); no caso das estaduais e federais, a mediana foi de, respectivamente, 337,0 (P25 - 75 = 281,2 - 524,2) e 370,0 (P25-75 = 300,0 - 480,0), Ӽ2(3) = 11,48, p = 0,009. As escolas não gratuitas tiveram mediana de CH total de SC de 500,0 horas (P25-75 = 336,0 - 690,0) e as gratuitas de 364,0 horas (P25-75 = 285,0 - 504,0), U = 4.259,0, z = - 3,26, p = 0,001. A mediana da CH, em horas, de epidemiologia e bioestatística AI entre 124 escolas foi de 88,0 (P25-75 = 60,0 - 120,0) e de saúde do trabalhador entre 63 foi de 40,0 (P25-75 = 33,0-60,0). Conclusões: A CH de SC AI apresenta grande variação, sendo maior em escolas não gratuitas.

Abstract: Introduction: The limits of the Community Health (CH) course load (CL) before medical clerkship (MC) in undergraduate medical courses are not defined neither in the National Curriculum Guidelines nor in other documents, and have not been investigated in a comprehensive way in national studies. Objective: To analyze the community health course load before clerkship in Brazilian medical schools. Method: Cross-sectional study of schools acknowledged by the Ministry of Education, which started activities until December 31, 2017. The sites of the 323 existing schools were accessed and the schools that had their curricula available in the internet with details of the CH CL before MC were included. The variables were school geographic region and administration type, medical course CL and CH CL before MC, also including epidemiology, biostatistics and occupational health course loads. Data were analyzed using descriptive statistics, Pearson chi-square (Ӽ2) for categorical variables and Mann-Whitney-U (U) and Kruskal Wallis Ӽ2 tests for continuous variables. Results: Out of 323 existing medical schools, 222 were included (68.7%). Among the included schools, 83 were tuition-free (37.4%) and 139 were not (62.6%). The median of the CH CL before MC was 440.0 hours (P25-75 = 300.0 - 640.0), equivalent to 5.4% (P25-75 = 3.5 - 7.8) of the medical course CL. The median of the CH CL before MC in hours in private and municipal schools was, respectively, 480.0 (P25-75 = 330.7 - 679.2) and 576.0 (P25-75 = 360.0 - 766.0), and of state and federal schools, respectively, 337.0 (P25-75 = 281.2 - 524.2) and 370.0 (P25-75 = 300.0 - 480.0), Ӽ2(3) = 11.48, p = .009. The median of the total CH CL in non-tuition-free schools was 500.0 hours (P25-75 = 336.0 - 690.0) and in the tuition-free schools, it was 364.0 hours (P25-75 = 285.0 - 504.0), U = 4.259.0, z = -3.26, p = .001. The median of epidemiology and biostatistics CL in hours before MC in the 124 schools was 88.0 (P25-75 = 60.0 - 120.0), whereas the occupational health CL in 63 schools was 40.0 (P25-75 = 33.0 - 60.0). Conclusions: The CH CL before clerkship varies widely, being higher in non-tuition-free medical schools.

ABSTRACTAvian metapneumovirus (aMPV) is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to theParamyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 µg mL-1) to inhibit the cytopathic effect (CPE) of the virus, expressed as inhibition percentage (IP). The maximum nontoxic concentration (MNTC) of the extracts used in antiviral assay was 25 µg mL-1for Aspidosperma tomentosumand Gaylussacia brasiliensis, and 2.5 µg mL-1for Arrabidaea chicaand Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica,and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action.

Efeitos de morfologia, espessura, oxidação e iluminação dos filmes de polipirrol dopados com dodecilsulfato podem ser observados qualitativamente por EIS e consistem de variações de valores de resistências e capacitâncias de um circuito equivalente proposto, presentes nas interfaces e no interior do material. O circuito se ajusta bem para filmes sintetizados com densidade de carga de 190 mC cm-2. Para filmes mais finos os valores calculados por espectroscopia de impedância eletroquímica (EIS) apresentam desvios provavelmente pela ausência de efeitos difusivos. A oxidação dos filmes causa uma diminuição geral da impedância em toda a faixa de freqüências medidas. Os efeitos de morfologia também são observados em todas as freqüências. Os efeitos de iluminação são reversíveis e aparecem, como esperado, apenas quando o polímero está na forma reduzida. A iluminação diminui a resistência interna e a capacitância de carga espacial e aumenta a resistência de transferência de carga e a capacitância de dupla camada.

The morphology, thickness, oxidation and illumination effects in dodecylsulphate doped polypyrrole films can be qualitatively observed by EIS and consist in variations of interfacial and bulk resistances and capacitances of a proposed equivalent circuit. The circuit well with the measured spectra of films obtained with 190 mC cm-2 of synthesis charge density. For thinner films the calculated values observed by electrochemical impedance spectroscopy (EIS) deviate probably due to the absence of diffusion effects. The oxidation of the films diminishes the total impedance over the entire frequency range. The morphology effects are also observed in the entire spectra. The illumination effects are reversible and are observed as expected only in the reduced form of the polymer. The illumination reduces the internal resistance and the space-charge capacitance and increases the charge transfer resistance and the double layer capacitance.