RESUMO: As microalgas são organismos cuja biomassa possui biomoléculas abundantes como carboidratos, lipídeos, proteínas, pigmentos, vitaminas, minerais e compostos antioxidantes, com inúmeras aplicações industriais com destaque para os segmentos de alimentos, nutricional, farmacológico, cosmético e de energia, na produção de biocombustíveis. Entretanto, o acesso de forma integra a essas biomoléculas pode ser dificultado pela rigidez estrutural de sua parede celular, sendo necessário a aplicação de um pré-tratamento que promova a lise celular. As diferentes técnicas de ruptura celular aplicáveis à biomassa microalgal descritas na literatura podem ser de natureza mecânica, com destaque para as técnicas de moagem em moinho de bolas e homogeneização à alta pressão (HAP) ou não mecânica (química, térmica e enzimática), cada uma com vantagens e desvantagens. Assim, o objetivo da presente revisão narrativa é descrever o uso de diferentes métodos para o rompimento de células de microalgas, com suas vantagens, desvantagens e seus desafios.

ABSTRACT: Microalgae are organisms whose biomass contains different biomolecules, such as carbohydrates, lipids, proteins, pigments, vitamins, minerals, and antioxidant compounds, with numerous industrial applications, highlighting the food, nutritional, cosmetic, pharmacological, and biofuel segments. However, access to these biomolecules in an integrated manner is often hampered due to the structural rigidity of their cell wall, requiring the application of a pre-treatment that promotes cell lysis. Various cell rupture techniques applied to microalgae biomass have been reported. These methods can be mechanical, especially ball milling techniques and high-pressure homogenization (HPH), or non-mechanical, such as chemical, thermal, and enzymatic procedures, each with advantages and disadvantages. Thus, this review addressed the different methods of cell disruption, listing their advantages and disadvantages, applications in cell biomass, and challenges.

The publication Cardiovascular Statistics – Brazil aims to provide an annual compilation of data and research on the epidemiology of CVDs in Brazil.  The report integrates official statistics from the Brazilian Ministry of Health and other governmental entities alongside data from the GBD project, coordinated by the IHME at the University of Washington. Additionally, it incorporates data derived from various sources and scientific studies, including cohorts and registries, that relate to CVDs and their associated risk factors. This publication is intended for a wide range of individuals, including researchers, clinicians, patients, healthcare policymakers, media professionals, the general public, and other interested parties seeking extensive national data about heart disease and stroke.  Volunteer researchers from various Brazilian universities and research institutions carry out the project. The group is led by a five-member steering committee (ALPR, CAP, DCM, GMMO, and LCCB). The Brazilian Society of Cardiology fully supports this initiative, and the project receives collaborative support from the GBD Brazil Network and an International Committee (GAR, PP, and TAG) from both the IHME/University of Washington (GAR) and the World Heart Federation (PP and TAG). 

A publicação Estatística Cardiovascular – Brasil tem por objetivo fornecer uma compilação anual dos dados e das pesquisas sobre a epidemiologia das DCV no Brasil.  Este documento integra as estatísticas oficiais do Ministério da Saúde do Brasil e outras entidades governamentais ao lado de dados do projeto GBD, coordenado pelo IHME da Universidade de Washington. Além disso, incorpora dados derivados de várias fontes e estudos científicos, inclusive coortes e registros, relacionados às DCV e fatores de risco associados. Esta publicação destina-se a um público variado, incluindo pesquisadores, clínicos, pacientes, formuladores de políticas de saúde, profissionais da mídia, o público em geral e todos aqueles que buscam dados nacionais abrangentes sobre DCV e acidente vascular cerebral. Pesquisadores voluntários de várias universidades e instituições de pesquisa brasileiros realizaram este projeto. O grupo é liderado por um comitê diretivo com cinco membros (ALPR, CAP, DCM, GMMO e LCCB). A Sociedade Brasileira de Cardiologia apoia integralmente esta iniciativa e o projeto recebe colaboração da Rede GBD Brasil1 e do GBD International Committee (GAR, PP e TAG) do IHME/Universidade de Washington (GAR) e da World Heart Federation (PP e TAG). 

Abstract: This study addresses the issue of equity in health and justice from the perspective of public health bioethics, describing the Brazilian Unified Health System (SUS), equating legitimate interests for essential goods, such as health. The conception of John Rawls’ theory of justice is “justice as fairness” and has a seventeenth century contractualism tenor. Although it was not conceived specifically for health and marked by the “difference principle”, it promoted, in the field of health care, the institution of health systems created on the basis of universal access and equity in the distribution of scarce resources. The principles of the Brazilian Unified Health System (SUS) guarantee access to all levels of care, equality in health care, without distinctions or privileges of any kind, integrity in health care, free of charge, community participation and decentralization, regionalization and hierarchization of health actions and services, which gives the SUS a strong Rawlsian bias. The Brazilian model was built on the principle that health is a right of all and a duty of the State, therefore, it is based on the assumption of universal and equal access to health actions and services for its promotion and recovery.

Resumen: Este estudio aborda el tema equidad en salud y justicia desde la perspectiva de la bioética de la salud pública, describiendo el Sistema Único de Salud (SUS) brasileño, equiparando intereses legítimos por los bienes esenciales, como la salud. La concepción de la teoría de justicia de John Rawls es la “justicia como equidad” y tiene un tenor de contractualismo del siglo XVII. Aunque no fue concebida específicamente para la salud y marcada por el “principio de la diferencia”, impulsó, en el ámbito de la atención sanitaria, la institución de sistemas de salud creados sobre la base del acceso universal y la equidad en la distribución de recursos escasos. Los principios del Sistema Único de Salud (SUS) brasileño garantizan el acceso para todos los niveles asistenciales, igualdad en la atención a la salud, sin distinciones ni privilegios de ningún tipo, integridad en la asistencia a la salud, gratuidad, participación comunitaria y una descentralización, regionalización y jerarquización de las acciones y servicios de salud, lo que da al SUS un fuerte sesgo rawlsiano. El modelo brasileño se construyó sobre el principio de que la salud es un derecho de todos y un deber del Estado, por lo tanto, se basa en el supuesto del acceso universal e igualitario a las acciones y servicios de salud para su promoción y recuperación.

Resumo: Este estudo aborda a questão da equidade em saúde e da justiça sob a perspectiva da bioética da saúde pública, descrevendo o Sistema Único de Saúde (SUS), equiparando interesses legítimos para bens essenciais, como a saúde. A concepção da teoria da justiça de John Rawls é “justiça como equidade” e tem um teor contratualista do século XVII. Embora não tenha sido concebida especificamente para a saúde e marcada pelo “princípio da diferença”, ela promoveu, no campo da assistência à saúde, a instituição de sistemas de saúde criados com base no acesso universal e na equidade na distribuição de recursos escassos. Os princípios do Sistema Único de Saúde (SUS) garantem o acesso a todos os níveis de atenção, a igualdade na assistência à saúde, sem distinções ou privilégios de qualquer espécie, a integralidade na assistência à saúde, a gratuidade, a participação da comunidade e a descentralização, regionalização e hierarquização das ações e serviços de saúde, o que confere ao SUS um forte viés rawlsiano. O modelo brasileiro foi construído com base no princípio de que a saúde é um direito de todos e um dever do Estado, portanto, parte do pressuposto do acesso universal e igualitário às ações e serviços de saúde para sua promoção e recuperação.

Abstract Objective: To evaluate the application of the Antimicrobial (ATM) treatment time reduction strategy in Intensive Care Units (ICU) in an Antimicrobial Stewardship Program (ASP). Method: This is a descriptive and cross-sectional study, carried out in two ICU of a university hospital in Fortaleza, Brazil, from January/2017 to January/2019. Adult patients were included, accompanied by a pharmacist, and using ATM, in which the treatment time reduction strategy was applied. The evaluation of the strategy was made through the difference between the predicted time established at the beginning of the treatment and the effective days of use of each ATM. Results: Of the 100 patients included, 51.0 % were male and 64.0 % were elderly. The respiratory system was the most frequently affected by the infections (37.4 %) and the most prevalent classes of ATM were carbapenems (23.0 %) and glycopeptides (20.1 %). There was a decrease from 831 unnecessary days of antimicrobial therapy and from an average of 13.7 to 8.9 days of treatment. The greatest reductions in days were observed for meropenem, with 202 days reduced. The study also allowed the identification of associations between the reduction > 8 days of treatment and the variables length of stay > 22 days and patients in exclusive palliative care, and associations between hospital discharge and reductions of up to 7 days of therapy. Conclusions: The data obtained suggest that the presence of an ASP influences the practices of ATM use and its treatment time and emphasize the role of pharmaceutical professionals in these programs.

Resumen Objetivo: Evaluar la aplicación de la estrategia reducción del tiempo de tratamiento Antimicrobiano (ATM) en Unidades de Cuidados Intensivos (UCI) en un Programa Stewardship de Antimicrobiano (ASP). Método: Este es un estudio descriptivo y transversal, realizado en dos UCI de un hospital universitario de Fortaleza, Brasil, de enero/2017 a enero/2019. Se incluyeron pacientes adultos, acompañados por un farmacéutico y utilizando ATM, en los que se aplicó la estrategia de reducción del tiempo de tratamiento. La evaluación de la estrategia se realizó a través de la diferencia entre el tiempo previsto establecido al inicio del tratamiento y los días efectivos de uso de cada ATM. Resultados: De los 100 pacientes incluidos, 51,0 % eran del sexo masculino y 64,0 % ancianos. El sistema respiratorio fue el más frecuentemente afectado (37,4 %) y las clases de ATM más prevalentes fueron los carbapenémicos (23,0 %) y los glicopéptidos (20,1 %). Hubo una disminución de 831 días innecesarios de terapia antimicrobiana y de un promedio de 13,7 a 8,9 días de tratamiento. Las mayores reducciones en días se observaron para meropenem, con 202 días reducidos. El estudio también permitió identificar asociaciones entre la reducción > 8 días de tratamiento y las variables estancia > 22 días y pacientes en cuidados paliativos exclusivos; y asociaciones entre alta hospitalaria y reducciones de hasta 7 días de terapia. Conclusiones: Los datos obtenidos sugieren que la presencia de un ASP influye en las prácticas de uso de ATM y su tiempo de tratamiento y enfatizan el papel de los profesionales farmacéuticos en estos programas.

ABSTRACT Introduction: Scientometrics is the field concerned with measuring and analyzing academic literature, using specific metrics and data from bibliometric databases. Hematology is a broad area of science and medicine, from which several landmark scientific discoveries have emerged. Objective: The aim of this report is to provide a snapshot of the landscape of hematology research in Brazil, based on a comprehensive analysis of published studies in hematology whose authors were affiliated to Brazilian institutions from 1980 to 2020. Method: Articles, reviews and letters to the editor with at least one author affiliated to a Brazilian institution were retrieved from Incytes/Web of Science or Scopus databases. Importantly, only papers classified in the subject area “Hematology” by the embedded algorithms of each database were included. Results: Considering all published papers, Brazil is in the 22nd position, contributing with around 1.1% of papers in this period. A clear and sustained increase in publication output can be observed from the early 1990’s to the present moment. Publicly-funded higher education institutions were the main contributors to the development and consolidation of the hematology scientific community, which has grown in diversity, with an increasing number of contributions from private institutions. In regard to funding, public agencies have been and remain by large as the most important funder of research in hematology in Brazil. Conclusion: We suggest that continuous monitoring of the temporal trends of some of the data compiled in our report could potentially contribute to a clearer picture of the development of hematology research in Brazil.

ABSTRACT Aedes (Stegomyia) albopictus (Skuse, 1854) was reported in Brazil for the first time in 1986 and has shown marked expansion throughout the Brazilian territory. During a routine activity to control dengue fever conducted by the Division of Entomology of the Municipal Health Department in Rio Branco city, adults and immatures of Culicidae were collected in a peri-urban area. The identified Culicidae forms indicated that they belonged to the species Ae. albopictus. This is the first official record of the presence of Ae. albopictus in the Acre State, confirming its current presence in all Brazilian states.

Abstract: Introduction: The onset of the Covid-19 pandemic led global health institutions to recommend the adoption of restrictive measures such as the closure of educational facilities. Educational institutions were forced to suddenly adapt to emergency distance learning methods, with little time for preparation. The students also faced challenges such as relying on the internet and remaining isolated at home. Medical and health science schools also had to adapt to this new model to humanized practices. Objective: To evaluate the perceived use of emergency distance learning by students at a state university health sciences and medical school during the Covid-19 Pandemic. Method: This is a descriptive and exploratory study, with a cross-sectional design and quantitative approach, involving the application of a questionnaire containing closed questions. The questionnaire was divided into four topics: sample characterization, conditions for internet use, study site conditions, and student perception of the use of distance learning. Result: The study included 480 students from the nursing, medicine, and dentistry courses at the Amazonas State University. Most of these students used their home wifi, with regular signal quality. Students from smaller, inner state towns were the most disadvantaged by inconsistent internet connections. Students were mainly in their bedrooms when they attended the and pointed to losing concentration easily as the main detrimental factor of the remote classes. Most students disagreed with the effectiveness of assessments for this type of learning,and rated the experience with remote teaching as unsatisfactory. However, most students agreed that it is possible to learn theoretical content with quality through distance learning. Conclusion: The Covid-19 pandemic brought about a sudden change of scenery. The new educational model, using distance learning, has brought new challenges for teaching. The results show that the factors that hindered the students were the instability of their internet connection and difficulty in maintaining concentration during distance learning classes. Students found it easy to clarify doubts about the classes with the teachers; the system proved adequate for the teaching and learning of the theoretical content.

Resumo: Introdução: Com a pandemia de Covid-19, instituições globais de saúde recomendaram a adoção de medidas restritivas, como o fechamento de unidades de ensino. As instituições de ensino tiveram que se adaptar ao ensino remoto emergencial de forma abrupta e com pouco tempo para preparo. Os alunos também tiveram desafios a serem enfrentados, como uso da internet e isolamento dentro de casa. O ensino superior na área de saúde também teve que se adequar a esse novo modelo com adaptação a práticas humanizadas. Objetivo: Este estudo teve como objetivo avaliar a percepção da utilização do ensino remoto emergencial por discentes em uma universidade pública de saúde durante a pandemia da Covid-19. Método: Trata-se de uma pesquisa descritiva e exploratória, com delineamento transversal e abordagem quantitativa, realizada por meio de aplicação de um questionário com perguntas fechadas. O questionário foi dividido em quatro tópicos: caracterização da amostra, condições de uso da internet, condições do local do estudo e percepção do estudante acerca da utilização do ensino remoto. Resultado: Participaram do estudo 480 alunos dos cursos de Enfermagem, Medicina e Odontologia da Universidade do Estado do Amazonas. A maioria desses alunos utilizou wi-fi residencial, com qualidade de sinal regular, e aqueles que residem no interior do estado foram os mais prejudicados por conta da inconstância da internet. Os alunos assistiram predominantemente às aulas no quarto e apontaram a fácil desconcentração como o fator mais prejudicial das aulas remotas. A maioria dos alunos discorda da eficácia das avaliações para esse tipo de ensino, assim como avalia de forma insatisfatória a experiência com o ensino remoto. No entanto, a maioria concordou que é possível aprender o conteúdo teórico com qualidade no ensino remoto. Conclusão: A pandemia de Covid-19 acarretou uma mudança repentina de cenário. O novo modelo educacional, de educação a distância, trouxe novos desafios para o ensino. Os resultados evidenciam que os fatores que prejudicaram os estudantes foram a instabilidade da internet e a dificuldade em se concentrar durante a aprendizagem remota. Houve facilidade para os discentes esclarecerem as dúvidas das aulas com os docentes, o que corrobora o ensino e a aprendizagem da parte teórica por meio desse sistema.

ABSTRACT: This article analyzed the international competitiveness of exports of forest products from 2008 to 2018. The information is available in the Food and Agriculture Organization (FAO) and the indicators used were: revealed comparative advantage (RCA), trade openness index (Oi) and the contribution to trade balance index (CTB). Results showed that the exportation of forest products are competitive, in addition gains in competitiveness have been observed by the RCA and in Europe, countries had losses in detriment of the financial crises and austerity measures adopted. All countries showed a low degree of openness by the Oi and had a comparative advantage by the CTB, except for China and Germany. It is concluded that the countries with bigger comparative advantages in the exportation of forest products were the ones that had contributed more for its commercial balance.

RESUMO: Este artigo analisou a competitividade internacional das exportações de produtos florestais, de 2008 e 2018. As informações estão disponíveis na Organização das Nações Unidas para Agricultura e Alimento (FAO) e os indicadores utilizados foram: vantagem comparativa revelada (RCA), o índice de abertura do comércio (Oi) e o índice de contribuição ao saldo comercial (CTB). Os resultados mostram que as exportações de produtos florestais são competitivas, ademais foram observados pelo RCA ganhos em competitividade e na Europa, os países tiveram perdas em detrimento das crises financeiras e medidas de austeridade adotadas. Todos os países apresentaram baixo grau de abertura pelo Oi e teve vantagem comparativa pelo CTB, com exceção da China e Alemanha. Conclui-se que os países com maiores vantagens comparativas nas exportações de produtos florestais foram os que mais contribuíram para o seu saldo comercial.

Abstract The aim of this study was to describe the prevalence, clinicopathological, and prognostic features of acinic cell carcinoma (AciCC) of the oral and maxillofacial region. AciCC cases were retrospectively retrieved from 11 pathology centers of three different countries. Medical records were examined to extract demographic, clinical, pathologic, and follow-up information. A total of 75 cases were included. Females (65.33%) with a mean age of 45.51 years were mostly affected. The lesions usually presented as an asymptomatic (64.28%) nodule (95.66%) in the parotid gland (70.68%). The association of two histopathological patterns was the most common finding (48.93%) and the tumors presented mainly conventional histopathological grades (86.11%). Surgical treatment was performed in the majority of the cases (59.19%). Local recurrence was observed in 20% of the informed cases, regional metastasis in 30.43%, and distant metastasis in 12.50%. The statistical analysis showed that the cases with a solid histopathological pattern (p=0.01), high-grade transformation (p=0.008), recurrence (p=0.007), and regional metastasis (p=0.03) were associated with poor survival. In conclusion, high histopathological transformation, presence of nodal metastasis, and recurrence were prognostic factors for AciCC of the oral and maxillofacial region.

ABSTRACT Saturated salt solutions produce environments with specific relative humidities used in several applications, such as determining water sorption isotherms of foods. However, the preparation of saturated solutions requires knowledge of solubility data. Thus, the objective of this work was to determine the solubility of 14 salts: lithium bromide (LiBr), lithium chloride (LiCl), lithium iodide (LiI), magnesium chloride (MgCl2), sodium iodide (NaI), potassium carbonate (K2CO3), magnesium nitrate (Mg(NO3)2), sodium bromide (NaBr), potassium iodide (KI), sodium chloride (NaCl), ammonium sulfate ((NH4)2SO4), potassium chloride (KCl), potassium nitrate (KNO3), and potassium sulfate (K2SO4) in the temperature range of 275.15 to 363.15 K at 101.3 kPa. The apparatus for the solubility determination consists of a jacketed glass cell and a magnetic stirrer. Furthermore, polynomial and exponential empirical models were fitted to the observed data to determine salt solubilities. Salt solubilities ranged from 7.39 to 80.41 g of salt·(100 g of solution)-1. Sodium iodide (NaI) presented the highest (p<0.05) solubility, up to 343.15 K, and lithium iodide (LiI) up to 363.15 K. The specific equilibrium moistures afforded by saturated saline solutions were associated with water activity data. A general mathematical model to determine the solubility of different salts was not obtained because the dissolution behaviors of each salt are distinct. However, the availability of solubility data of saline solutions covering a wide range of temperatures is helpful since the salts have different behavior.

RESUMO Soluções salinas saturadas produzem ambientes com umidades relativas específicas utilizadas em diversas aplicações, como na determinação de isotermas de sorção de água de alimentos. No entanto, a preparação de soluções saturadas requer conhecimento de dados de solubilidade. Assim, o objetivo deste trabalho foi determinar a solubilidade de 14 sais: brometo de lítio (LiBr), cloreto de lítio (LiCl), iodeto de lítio (LiI), cloreto de magnésio (MgCl2), iodeto de sódio (NaI), carbonato de potássio (K2CO3), nitrato de magnésio (Mg(NO3)2), brometo de sódio (NaBr), iodeto de potássio (KI), cloreto de sódio (NaCl), sulfato de amônio ((NH4)2SO4), cloreto de potássio (KCl), nitrato de potássio (KNO3) e sulfato de potássio (K2SO4) na faixa de temperatura de 275,15 a 363,15 K a 101,3 kPa. O aparelho para a determinação da solubilidade consiste em uma célula de vidro encamisada e um agitador magnético. Além disso, modelos empíricos polinomiais e exponenciais foram ajustados aos dados observados para determinar solubilidades de sal. A solubilidade do sal varia de 7,39 a 80,41 g de sal·(100 g de solução)-1. Iodeto de sódio (NaI) apresentou o maior (p<0.05) solubilidade, até 343,15 K, e iodeto de lítio (LiI) até 363,15 K. Específicas umidades de equilíbrio proporcionadas pelas soluções salinas saturadas foram associadas aos dados de atividade de água. Um modelo matemático geral para determinar a solubilidade de diferentes sais não foi obtido porque os comportamentos de dissolução de cada sal são distintos. No entanto, a disponibilidade de dados de solubilidade de soluções salinas cobrindo uma ampla faixa de temperaturas é útil, pois os sais têm comportamento diferente.

Abstract This paper discusses the process of spatial transformation in the city of Manaus from the second half of the nineteenth century as it aspired to modernity; this process was initially driven by the elevation of Amazonas to the category of province (with Manaus as the capital), and later by the wealth resulting from the rubber trade. Here, these transformations focus on cemetery space as part of a larger scenario of changes throughout the country as medical and sanitary precepts defending control measures to make urban space healthier became more widely disseminated. This discourse was in line with urban reforms that were becoming more solidified in Europe at the time, demonstrating efforts to adapt to what was believed to be the path to progress.

Resumo Este artigo aborda o processo de transformação espacial vivido pela cidade de Manaus a partir da segunda metade do século XIX, em sua aspiração à modernidade, primeiro impulsionada pela elevação do Amazonas à categoria de província, da qual Manaus era agora capital, depois pela prosperidade decorrente do comércio da borracha amazônica. Aqui, estas transformações têm como foco o espaço cemiterial, inserindo-se num quadro maior de mudanças vivido em todo o país, com a difusão de preceitos médico-sanitaristas que defendiam medidas de controle visando à salubridade do espaço urbano, discurso alinhado com reformas urbanas em consolidação na Europa, mostrando a busca pela adequação ao que se acreditava ser o caminho do progresso.

ABSTRACT: The present study investigated Salmonella spp. in the feces of 200 foals up to one year of age (100 with clinical signs of diarrhea and 100 without clinical signs of diarrhea). Bacteriological culture, serotyping, antimicrobial susceptibility, and real-time PCR (qPCR SYBR® Green or a TaqMan®) for detecting the invA gene (with and without a selective pre-enrichment step in tetrathionate broth) were performed. Bacterial culture revealed 15% (n=30) of positive animals (21 animals with diarrhea and nine without diarrhea). Among the 30 isolates, 13 different serovars were identified: S. Infantis, S. Minnesota, S. I.4,5,12:i:-; S. Anatum, S. Cerro, S. Oranienburg, S. Braenderup, S. Give, S. Newport, S. IIIb 61:c:z35, S. 109:-:1.5, S. I.4.12:d:-, S. I.6.8:-:-. Multidrug resistance was found in 43.33% (n=13) of the isolates, with one isolate obtained from animals without diarrhea and 12 isolates from animals with diarrhea. All qPCR techniques used in the study classified more samples as positive for Salmonella spp. than the bacterial culture of feces. In addition, all qPCR techniques detected more positive animals in the diarrhea group than in the diarrhea-free group. The results confirm the utility of the qPCR method without the pre-enrichment step in tetrathionate as a rapid test for Salmonella spp. in carrier animals. In animals with clinical signs of diarrhea, it can be combined with bacterial culture (antimicrobial susceptibility testing and serotyping). The isolation of Salmonella spp. in nine animals without diarrhea confirms the importance of asymptomatic carrier animals in the epidemiology of the disease. The multidrug resistance observed highlights the importance of rational antimicrobial use in horses and adopting biosecurity protocols that are efficacious in controlling the spread of infections between animals and zoonotic transmission in farms.

RESUMO: O presente estudo investigou a ocorrência de Salmonella spp. em fezes de 200 potros com até um ano de idade (100 com sinais clínicos de diarreia e 100 sem sinais clínicos de diarreia), utilizando as técnicas de cultivo bacteriológico e PCR em tempo real (qPCR) pelos métodos de corante fluorescente (SYBR® Green) e sonda específica (Taqman®) para a detecção do gene invA com e sem etapa de pré-enriquecimento seletivo em caldo de tetrationato. O cultivo bacteriológico revelou 15% (n=30) de animais positivos (21 animais com diarreia e nove animais sem diarreia). Dentre esses 30 isolados, 13 sorovares diferentes foram identificados: S. Infantis, S. Minnesota, S. I.4,5,12:i:-; S. Anatum, S. Cerro, S. Oranienburg, S. Braenderup, S. Give, S. Newport, S. IIIb 61:c:z35, S. 109:-:1.5, S. I.4.12:d:-, S. I.6.8:-:-. Multirresistência foi constatada em 43,33% (n=13) dos isolados, sendo um isolado obtido de animal sem diarreia e 12 isolados de animais com diarreia. Todas as técnicas de qPCR empregadas no estudo apresentaram maior número de amostras classificadas como positivas para Salmonella spp. comparadas ao cultivo bacteriológico de fezes. Adicionalmente, em todas as técnicas de qPCR houve maior número de animais detectados como positivos no grupo de animais com diarreia em relação aos animais sem diarreia. Os resultados confirmaram a utilidade do método qPCR sem a etapa de pré-enriquecimento em tetrationato, como um teste rápido para detecção de Salmonella spp. em animais portadores ou em animais com sinais clínicos de diarreia. O cultivo bacteriológico deve ser associado para a realização do teste de sensibilidade aos antimicrobianos e sorotipificação. O isolamento de Salmonella spp. em nove animais sem diarreia, confirma a importância dos animais portadores assintomáticos na epidemiologia da doença. A multirresistência observada evidencia a importância do uso racional de antimicrobianos em equinos e a importância da adoção de protocolos de biossegurança que sejam eficazes para controlar a disseminação de infecções entre animais e a transmissão zoonótica nas fazendas.

Abstract The present study verified the presence of phytoconstituents and evaluated antioxidant (DPPH, FRAP, NO and TBARS tests) and antiglycation (REM test) activities of unconventional wild edible fruits Chrysophyllum cainito, Hancornia speciosa and Plinia glomerata. It was verified the presence of phenolic compounds for all fruits and flavonoids were observed only for C. cainito, which presented in its peel the highest total phenols (90.34 μg GAE mg-1) and flavonoids (30.4 μg RE mg-1) content. Sugar concentration was significant for all fruits, where H. speciosa showed the highest reducing sugar content (576.12 mg g-1) and C. cainito pulp showed the highest total sugar content (858.67 mg g-1). All fruits presented vitamin C and carotenoids, highlighting P. glomerata with the best results for ascorbic acid (2260.94 mg 100 g-1) and carotenoids (59.62 µg g-1). Extracts presented antioxidant activity, highlighting C. cainito peel that presented 65.64% (DPPH), 231.34 µM TE L-1 (FRAP), 49.34% (NO) and 22.56% (TBARS), while in antiglycation evaluation, P. glomerata showed evident activity. Therefore, it was possible to determine different phytoconstituents, and antioxidant and antiglycation activities of the fruits. These data provide subsidies for application of these fruits in new studies, to increase knowledge and preservation of these species.

This research was aimed to obtain biodegradable polymeric films with antifungal action against Aspergillus niger, through the addition of copper oxide nanoparticles (CuO-NPs) in the commercial polymer (Ecoflex®) and to evaluate its mechanical, morphological, biodegradation, and copper release properties. The microbiological results showed that the films with 4 wt% CuO-NPs inhibited 97.57% the growth of the fungus growth of 1×105 spores·mL-1 compared to the control. The morphological characterization revealed that the CuO-NPs presented irregular form with particles size of 50 to 400 nm and the XRD showed characteristic peaks of copper oxide. The addition of CuO-NPs to Ecoflex® provided increased maximum and rupture tension, strain, and elasticity modulus the obtained films with 1 wt% CuO-NPs and did not alter the biodegradability of the films. It can be said that, through the addition of CuO-NPs to Ecoflex®, it was possible to add antifungal activity for Aspergillus niger without compromising its biodegradability, meeting the safety standards established by legislation regarding the release of copper.