RESUMO. A biomassa dos microrganismos do solo é um importante indicador da qualidade ambiental. Esta biomassa é determinada matando os microrganismos por fumigação com clorofórmio, irradiações gama ou micro-ondas. Quatro solos com concentrações crescentes de carbono (5, 10, 15 e 30 g kg-1) foram submetidos a quatro métodos: clorofórmio fumigação ou aplicação direta; irradiação em micro-ondas; e irradiação gama de 15, 25, 35, 45 e 60 KGy. Fungos e bactérias foram quantificados por contagem em placa. Micro-ondas e irradiação gama ≥ 25 Gy mataram todos os microrganismos, mas não os métodos com clorofórmio. O C liberado aumentou com as doses de irradiação, apesar da mortalidade semelhante, indicando liberação de C da matéria orgânica além da microbiana sobrestimando a biomassa microbiana. As biomassas determinadas com micro-ondas correlacionaram-se com as da fumigação e irradiação com 25 KGy mas os valores diferiram entre os métodos em pelo menos um solo. Apesar da discrepância entre métodos, todos foram consistentes em ranquear as biomassas em ordem crescente das concentrações de C do solo, que corresponde a intensidades de uso decrescentes.

ABSTRACT. Soil microorganism biomass is an important soil quality indicator. The microbial biomass of soil was determined by killing and lysing the soil microbes by fumigation with chloroform, irradiation with gamma rays, or irradiation with microwaves. Four soils with increasing carbon concentrations (5, 10, 15, and 30 g kg-1) were analyzed using four methods: the direct application of chloroform, chloroform fumigation, microwave irradiation, and gamma ray irradiation with radiation doses of 15, 25, 35, 45, and 60 KGy. The fungi and bacteria in the soil were quantified by plate counting. Microwave irradiation and gamma irradiation with doses equal to or above 25 KGy killed all the soil microorganisms, but the chloroform methods did not. The carbon liberation increased with higher gamma doses, while the microbe mortality rates were the same, indicating that carbon was liberated from organic matter sources other than microorganisms. The biomasses determined by the microwave method correlated with those determined by the fumigation and 25 KGy gamma irradiation methods, but their values differed among all methods for at least one soil type. Despite this discrepancy, all methods were consistent in ranking microbial biomasses in increasing order of soil carbon concentrations, which corresponds with decreasing land use intensities.

The area of Guaratiba, in Rio de Janeiro, presents extraordinary population growth rates that exceed all other districts of the city. Moreover, the public investments underway, in view of the 2106 Olympic Games, are making the region even more attractive. Therefore, it is appropriate to suggest proactive measures to avoid the predicted collapse of several public systems among them the education system. This paper considers the projected population for the years 2015 and 2020 and, using various computing resources, specially the ArcGIS Network Analyst tool for measuring traveled distances, proposes locating new facilities with the Capacitated p-Median Model and with the Maximum Covering Location Problem, considering an ideal maximal home-school distance of 1,500 meters, but also evaluating longer distances. Both problems have been solved with AIMMS. The consideration of both models provides a constructive insight that certainly improves the implemented solution and favors the local community.

O objetivo deste trabalho foi avaliar o efeito de glifosato sobre a germinação de esporos de Phakopsora pachyrhizi e no controle da ferrugem da soja, aplicado preventiva e erradicativamente, em condições controladas. A germinação de esporos foi avaliada tendo-se vertido, em meio de cultura, soluções de esporos com diferentes concentrações do glifosato (0, 100, 1.000, 10.000 e 20.000 ppm) e fungicidas. Para avaliar o controle da ferrugem, plantas foram pulverizadas com glifosato, com variação da dose e do momento, tendo-se medido a severidade da doença. Houve efeito do produto sobre os esporos do fungo, o que reduziu sua germinação. As pulverizações em plantas, em casa de vegetação, mostraram um efeito do produto sobre a ferrugem, quando aplicado preventivamente, porém com período de proteção curto e fungitoxicidade inferior à do fungicida tebuconazole. O uso do glifosato, avaliado nesse ensaio, não deve ser visto como medida de manejo de ferrugem, e não interfere nas práticas habituais de controle da ferrugem da soja.

The objective of this work was to evaluate the effect of glyphosate on the germination of Phakopsora pachyrhizi spores, and on soybean rust control; applications were either preventive or eradicative under controlled conditions. The germination of spores was evaluated by pouring spores solutions on culture medium, with different concentrations of glyphosate (0, 100, 1,000, 10,000 and 20,000 ppm) and fungicides. For the rust control evaluation, plants were sprayed with glyphosate, varying doses and moments, and estimating the disease severity. The product reduced spores germination. Plants sprayed in greenhouse conditions, showed effect of the product on rust, when applied preventively, but with short protection period and lower fungus toxicity than tebuconazole fungicide. The use of glyphosate, evaluated in this trial, must not be taken as a rust management measure, and should not interfere in usual practices for soybean rust control.