ABSTRACT Extracts of the seeds of Tephrosia vogelii Hook. f. were studied in relation to its chemical composition and toxicity to the brown stink bug Euschistus heros (F.). The extracts were obtained in ethyl acetate and ethanol in the sequence according to the polar nature of the solvents. Extracts were sprayed in concentration of 1.0, 2.5, 5.0, 7.5 and 10% on third-instars nymphs and adults, and mortality was recorded. Presence two rotenoids in ethyl acetate was detected, with analyzed with gas chromatography-mass spectrometry (GC-MS). Crude fraction analyses confirmed the presence of these rotenoids (tephrosin - 2.71% in ethyl acetate and 3.66% in methanol; and deguelin - 10.46% in ethyl acetate and 1.22% in methanol) and three other rotenoids in small amounts. Eight days after applications, ethyl acetate caused more stink bugs mortality and on less time than ethanol extract, because great quantity of rotenoids, as polarity. Concentrations above to 1 and 2.5% of the ethyl acetate extracts caused mortality above 80% of the nymphs and adults of E. heros, respectively. Concentration were considered high, thus chemist analyzes demonstrated high rotenoids presence. In conclusion, seed T. vogelli extracts, rich in deguelin and tephrosin (3:1), cause mortality of E. heros, however, high concentration are necessary.

ABSTRACT We evaluated the influence of host preference, mating, and release density on Telenomus remus (Nixon, 1937) (Hymenoptera: Platygastridae) parasitizing eggs of Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae). First, we tested host preference of T. remus (free choice test) offered a choice between eggs of Corcyra cephalonica (Stainton, 1865) (Lepidoptera: Pyralidae) and S. frugiperda. Parasitism capacity and host preference (S. frugiperda) of T. remus reared on either of the two hosts did not differ. Secondly, we evaluated the influence of mating behavior of T. remus females on its parasitism. Only the offspring sex ratio differed between treatments, indicating that the species reproduces by parthenogenesis of the arrhenotoky type. Finally, we evaluated the influence of release density on T. remus parasitism. This was tested by releasing different numbers of the parasitoid per S. frugiperda egg using T. remus reared for different numbers of generations on C. cephalonica eggs. The regression analysis between percentage of parasitism and density of released T. remus females showed a quadratic effect for all tested parasitoid generations (F35, F40, and F45) with maximum parasitism from 65.07% to 71.69%. Our results allow the conclusion that (a) T. remus prefers S. frugiperda eggs, regardless of the host on which this parasitoid was reared, showing no preimaginal conditioning; (b) Mating does not affect the number of eggs parasitized by T. remus or the development of its offspring; and (c) The optimal T. remus release density when reared on C. cephalonica is between 0.133 and 0.150 females/S. frugiperda.

Lavouras de morango recebem várias aplicações de agrotóxicos e resíduos nos frutos são recorrentes. O desenvolvimento e a aplicação de métodos de manejo que evitem a aplicação de pesticidas, principalmente durante o período de frutificação, é uma necessidade iminente. Compararam-se as populações do ácaro rajado Tetranychus urticae Koch. em plantas adubadas com fertilizantes orgânicos e minerais (como o usados por produtores) e tratadas com produtos alternativos. As plantas foram cultivadas em vasos, em casa de vegetação infestada de população local do ácaro. Extratos de Tephrosia voggelii (Hook), óleo de nim (Azadirachta indica A. Juss.) e calda sulfocálcica foram pulverizados três vezes em intervalos de 10 dias. As avaliações foram realizadas três vezes após cada pulverização. A interação entre a fertilização e produtos alternativos em pulverização foi também avaliada, mas não foi significativa. A população do ácaro foi cerca de 44% menor em plantas fertilizadas com fertilizante orgânico do que aquelas com fertilizante mineral. Extrato de T. voggelii reduziu em grande quantidade a população do ácaro; o óleo de nim reduziu em algumas avaliações e redução intermediária foi observada com calda sulfocálcica. Adubação orgânica e extrato de T. voggeli são estratégias potenciais para manejar a população de ácaros e estudos adicionais são propostos para permitir ampla utilização

Strawberry fields receive several pesticides spraying and residues in fruits are recurrent. Development and application of methods that avoid applying pesticides mostly during fructification period are imminent needs. Comparison of population of two-sppoted spider mite (TSSM) Tetranychus urticae Koch. in organic and mineral fertilized plants (as used by growers) and treated with alternative products were done. Plants were grown in vessels in a greenhouse infested with local population of TSSM. Extracts of Tephrosia voggeli (Hook), neem oil (Azadirachta indica A. Juss.), and lime sulfur were applied three times against TSSM in ten days intervals. Assessments were done three times after each spraying. Interaction between fertilization and spraying alternatives was also assessed but was not significant. TSSM population was about 44% lesser in organic fertilized plants than those in inorganic fertilized. T. voggeli extracts reduced in large amounts TSSM; neem oil reduced in some assessments and an intermediate reduction was found for sulfur lime. Organic fertilization and T. voggeli extracts are potential strategies to manage TSSM and additional studies are proposed to enable wide utilization

The genomes of 10 equine herpesvirus 1 (EHV-1) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, BamHI and BglII, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype.