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ABSTRACT Zinc is an essential micronutrient for plant metabolism and has been studied in several crops of economic interest. However, there is no evidence of its use in coconut crops. The objective of this study was to evaluate the influence of foliar zinc fertilization with doses of zinc on dwarf green coconut seedlings based on their nutritional status, growth attributes, and gas exchange. The experimental design consisted of randomized blocks, and the treatments consisted of different zinc doses (0, 5, 10, 15, 20, and 25 g per plant) with five replicates. Seedlings were positively influenced by zinc dose, mainly in terms of growth variables and gas exchange. Zinc fertilization reduced iron and manganese content and increased zinc concentrations in the leaf tissue. Zinc fertilization promoted an increase in leaf area, height, shoot dry mass, and total dry mass. Furthermore, zinc fertilization improved photosynthesis, transpiration, stomatal conductance, internal CO2 concentration, instantaneous water use efficiency, and intrinsic water use efficiency. The improvement in these growth indices and gas exchange, in most variables, was up to an estimated dose of approximately 16 g of zinc per plant and decreased with increasing doses.

RESUMO O zinco é um micronutriente de importância essencial para o metabolismo vegetal, é estudado para diversas culturas de interesse econômico, porém sem evidências de estudo para a cultura do coqueiro. Objetivou-se com este estudo avaliar a influência da fertilização com doses de zinco via foliar em mudas de coqueiro anão verde, por meio do estado nutricional, atributos de crescimento e trocas gasosas. O delineamento experimental foi em blocos ao acaso, e os tratamentos foram constituídos por doses de zinco (0, 5, 10, 15, 20 e 25 g por planta), com cinco repetições. As mudas foram influenciadas positivamente pelas doses de zinco principalmente nas variáveis de crescimento e nas trocas gasosas. No estado nutricional afetou de maneira decrescente o ferro e o manganês e aumentou o zinco no tecido foliar. O zinco proporcionou incrementos na área foliar, altura de plantas, massa seca da parte aérea e massa seca total. Além disso, melhorou a fotossíntese, transpiração, condutância estomática, concentração interna de CO2, eficiência instantânea do uso da água e eficiência intrínseca do uso da água. A melhora nesses índices de crescimento e trocas gasosas, na maioria das variáveis, foi até a dose estimada de aproximadamente 16 g de zinco por planta e diminuiu com o aumento das doses.

https://doi.org/10.1590/1807-1929/agriambi.v27n11p864-872

Characterization of Flavonoids Profiles in Polar Extracts from Croton grewioides Baill. Using Ultra-High Resolution Mass Spectrometry Baill UltraHigh Ultra High
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Nascimento, Pedro E. S. do

; Prado, Vilma M. J.

; Jesus, Raphael A. de

; Silva, Wenes R. da

; Wisniewski Jr., Alberto

; Sá Filho, José C. F.

; Blank, Arie F.

; Souza, Daniel A. de

; Wartha, Elma R. S. A.

; Nogueira, Paulo C. L.

; Moraes, Valéria R. S.

Journal of the Brazilian Chemical Society

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Jul 2023, Volume 34 Nº 7 Páginas 937 - 948

Resumo: En | Texto: En | PDF: En

Research to identify plant bioactive compounds led to the evolution of extraction methods. This study optimized ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) to extract the flavonoids of Croton grewioides Baill. species contributing to characterize the polar extract profile and understand the antioxidant potential of these plant constituents. The use of experimental design and statistical treatments enabled the determination of the ideal conditions for each technique. The extracts of five accessions of C. grewioides were obtained under the optimized extraction conditions and were analyzed using ultra high-resolution mass spectrometry (FT-Orbitrap MS) operating at negative ionization mode for flavonoids detection. The accurate experimental mass obtained to the main compounds was used to attribute the molecular formula. Chemical structures of the main compounds detected were proposed using structure data bases. Chemometric analysis were performed with two FT-Orbitrap MS spectra samples using the identified metabolites and, the antioxidant activity data, showing that for this species the MAE was most effective in extracting the antioxidant compounds. It was possible to propose the structures for forty compounds in the C. grewioides extracts, demonstrating the excellent performance of the FT-Orbitrap MS in providing information on the chemical profile of polar compounds in plant extracts. methods ultrasoundassisted ultrasound assisted UAE (UAE microwaveassisted microwave (MAE Baill constituents technique C highresolution high resolution FTOrbitrap FT Orbitrap detection formula bases

https://doi.org/10.21577/0103-5053.20230004

First record of Sebekia oxycephala (Pentastomida: Sebekidae) infecting Helicops angulatus (Reptilia: Colubridae) in Northeast Brazil
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Oliveira, M. C.

; Ferreira-Silva, C.

; Silva, R. J.

; Almeida, W. O.

; França, F. G. R.

; Lourenço-de-Moraes, R.

Brazilian Journal of Biology

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2023, Volume 83 elocation e275302

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract In this study, we investigated the infection by pulmonary parasites in the water snake Helicops angulatus. We found four nymphs of the pentastomide parasite Sebekia oxycephala, parasitizing two adult females H. angulatus collected in the municipality of Rio Tinto, Paraíba, Brazil. This occurrence is likely due to the nymphs of this parasite directly infecting aquatic intermediate hosts, such as fish. The result of this study represents the first record of pentastomid infecting H. angulatus.

Resumo Neste estudo investigamos a infecção por parasitos pulmonares na serpente Helicops angulatus. Encontramos quatro ninfas de pentastomídeos (Sebekia oxycephala) parasitando duas fêmeas adultas de H. angulatus oriundas do município de Rio Tinto, Paraíba, Brasil. A ocorrência das ninfas na serpente sugere que a transmissão ocorra através da cadeia trófica, uma vez que peixes atuam como hospedeiros intermediários do parasito e fazem parte da dieta H. angulatus . O resultado deste estudo representa o primeiro registro de pentastomídeos infectando H. angulatus.

https://doi.org/10.1590/1519-6984.275302

Growth and production of cowpea beans under potassium doses in soil of cerrado in Amapá, Brazil
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Costa, K. S. Q.

; Oliveira, C. F.

; Melo, M. P.

; Lima, H. C.

; Ferreira, R. L. C.

; Melo, N. C.

; Moraes, F. K. C.

; Cruz, F. J. R.

; Souza, L. C.

; Nascimento, V. R.

Brazilian Journal of Biology

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2023, Volume 83 elocation e273777

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract The cowpea bean [Vigna unguiculata (L.) Walp.], a legume of great socioeconomic importance, it was previously cultivated exclusively for subsistence and commercial purposes, especially in the North and Northeast regions. This crop has a low production cost and high nutritional value, in addition to a high potential for productivity growth and expansion to other regions. The objective of this work was to evaluate parameters of growth and production in cowpea culture, as a function of potassium fertilization in soil of the cerrado of Amapá. The parameters of growth and production of the cowpea culture were evaluated, as a function of potassium fertilization in the soil of the cerrado of Amapá. The experiment was conducted in a greenhouse, using a completely randomized experimental design, with four replications, in a 5x2 factorial scheme, totaling 40 experimental units, which were composed of plastic pots containing 7 dm3 of soil collected from the arable layer (0-20 cm ) of a typical Hyperdystrophic Yellow Argisol, with a sandy clay loam texture, in a cerrado area in the municipality of Porto Grande-AP. The factors consisted of the control treatment (without K), four doses of K (45, 90, 135 and 180 kg ha-1) in the form of potassium chloride, and two cowpea cultivars (Pretinho and BRS Tumucumaque). The cultivar BRS Tumucumaque shows better growth and production of cowpea plants. Doses of 90 kg ha-1 provided greater height (98.75 cm) and stem diameter (10.0 mm). As for production, the dose of 135 kg ha-1 caused greater grain weight gain (5.25 g) and dry mass of pods (13.92 g), and the doses of 90 and 180 kg ha-1 induced greater number and length of pods (3.16 pods) respectively. These results show better responsiveness of the BRS Tumucumaque cultivar at doses of 90, 135 and 180 kg ha-1 in the type of soil where the study was conducted.

Resumo O feijão-caupi [Vigna unguiculata (L.) Walp.], leguminosa de grande importância socioeconômica, era anteriormente cultivado exclusivamente para fins de subsistência e comerciais, principalmente nas regiões Norte e Nordeste. Essa cultura apresenta baixo custo de produção e alto valor nutritivo, além de alto potencial de crescimento de produtividade e expansão para outras regiões. Apesar da importância fisiológica do potássio para o crescimento das plantas e o plantio da cultura ocorrer predominantemente em solos arenosos e argilosos, há poucos estudos relacionando seu cultivo à adubação potássica. O objetivo deste trabalho foi avaliar parâmetros de crescimento e produção na cultura do feijão-caupi, em função da adubação potássica em solo do cerrado do Amapá. Foram avaliadas a altura das plantas, o diâmetro do caule, a massa seca da parte aérea (folha e caule) e da raiz e a produção (massa seca de grãos e vagens, comprimento de vagens e número de vagens) na cultura do feijão-caupi em função da adubação potássica no solo do Cerrado. do Amapá. O experimento foi controlado em casa de vegetação, utilizando delineamento experimental inteiramente casualizado, com quatro repetições, em esquema fatorial 5x2, totalizando 40 unidades experimentais, as quais foram compostas por vasos plásticos contendo 7 dm3 de solo coletado da camada arável (0-20 cm) de um típico Argissolo Amarelo Hiperdistrófico, com textura franco-argilosa arenosa. Os fatores foram constituintes do tratamento controle (sem K), quatro doses de K (45, 90, 135 e 180 kg ha-1) na forma de potássio potássico e duas cultivares de feijão-caupi (Pretinho e BRS Tumucumaque). A cultivar BRS Tumucumaque apresentou melhor crescimento e produção. A dose de 90 kg ha-1 proporcionou maior altura (98,75 cm) e diâmetro do caule (10,0 mm). Quanto à produção, a dose de 135 kg ha-1 proporcionou maior ganho de peso de grãos (5,25 g) e massa seca de vagens (13,92 g), e doses de 90 e 180 kg ha-1 induziram maior número e comprimento de vaginas (3,16 vaginas), respectivamente. Esses resultados mostram melhor responsividade da cultivar BRS Tumucumaque nas doses de 90, 135 e 180 kg ha-1 no tipo de solo onde o estudo foi realizado.

https://doi.org/10.1590/1519-6984.273777

Volatile organic compounds (VOCs) of essential oils for the control of Fusarium oxysporum in cherry tomato seeds
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Schuster-Russiano, M. C.

; Russiano, C. G. S.

; Moraes, P. V. D.

; Ducatti, R. D. B.

; Mazaro, S. M.

Brazilian Journal of Biology

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2023, Volume 83 elocation e274368

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Fusarium oxysporum is the causal agent of Fusarium wilt in tomato plants. The most common form of control of this disease is through seed chemical treatment. However, the present work presents an alternative method, through the fumigation technique with essential oils. The pathogen F. oxysporum was inoculated on organic cherry tomato seeds through contact with sporulated Petri® plates. Thereafter, seeds were placed in stainless steel crucibles containing a 1.0 x 1.0 cm filter paper adhered to the lid and kept for 24 hours. This paper received 20 µL of each essential oil: tea tree, chia, citronella, lavender, anise basil, clove basil, and deionized water as control. This process was called “seed fumigation by essential oil”. After this process, a germination test was carried out in germ boxes with Germitest® paper to verify the variables Germination Speed Index (GSI), Germination (G%), and Mean time to germination (MGT). Mycelial growth was verified in Petri® plates containing PDA medium. The plates containing mycelial growth were observed through scanning electron microscopy to verify possible morphological damage in the hyphae of the pathogen. Tea tree essential oil was the one that allowed the greatest suppression of the phytopathogen. Therefore, new tests were carried out with this specific oil. In germ boxes, tests of germination (G%), Abnormal seedlings count (ASC), and percentage of seedlings with mycelial growth were carried out. In addition, plant elicitation tests were performed in tomato seedlings through the analysis of chitinase, glucanase, and total proteins. All tests were carried out in completely randomized designs with four replications. All data were submitted to the Lilliefors normality test, followed by the analysis of variance, and Tukey’s HSD (5% significance) for mean comparison. It was found that tea tree essential oil inhibited the mycelial growth of F. oxysporum without affecting the germination of cherry tomato seeds. Subsequent tests with this oil also demonstrated that there is a reduction in mycelia present in the seeds and a reduction in abnormal seedlings compared to the control. There was no significant difference between the variables tested for plant elicitation.

Resumo Fusarium oxysporum é o agente causal da murcha de Fusarium em tomateiro, a forma mais comum de controle da doença é através do tratamento químico das sementes, porém o presente trabalho apresenta uma alternativa, através da técnica de expurgo com óleos essenciais. O patógeno, Fusarium oxysporum, foi inoculado em sementes de tomate cereja orgânicas, através do contato com placas de Petri® esporuladas. Em seguida, estas sementes dotadas do inóculo da doença, foram alocadas em cadinhos de inox contendo um papel filtro de 1 cm x 1cm, aderido à tampa contento 20 uL de cada óleo: óleo essencial de melaleuca, chia, citronela, lavanda, alfavaca anis, alfavaca cravo e água deionizada como testemunha. Esse processo em que as sementes foram alocadas em cadinhos contendo óleo essencial na tampa por um período de 24 horas, foi chamado de expurgo das sementes pelo óleo essencial. Após este processo, foi realizado o teste de germinação em caixas Gerbox com papel Germitest®, verificando as variáveis IVG, %G, TMG; e crescimento micelial em placas de Petri® contendo meio BDA. As placas de crescimento micelial foram observadas em microscópio eletrônico de varredura, a fim de verificar possíveis danos morfológicos nas hifas do patógeno. Após tais ensaios, constatou-se que o óleo essencial de melaleuca foi o que obteve maior supressão do fitopatógeno; e dessa forma novos ensaios laboratoriais foram realizados apenas com este óleo. Em caixas Gerbox realizou-se o teste de germinação (%), contagem de sementes anormais e porcentagem de sementes com micélio, além de testes de indução de resistência em plântulas de tomate, realizado por meio da análise de quitinase, glucanase e proteínas totais. O delineamento adotado em todos os ensaios foi o inteiramente ao acaso, com quatro repetições. Os dados obtidos foram submetidos ao teste de normalidade de Lilliefors, e à análise de variância, com as médias comparadas pelo teste de Tukey (significância de 5%). Constatou-se que o óleo de melaleuca inibiu o crescimento micelial de F. oxysporum, sem prejudicar a germinação das sementes de tomate cereja. Os testes subsequentes com este óleo, também demonstram que há a redução de micélio nas sementes e redução de plântulas anormais em comparação com a testemunha. Não ocorreu diferença significativa entre as variáveis testadas para indução de resistência.

https://doi.org/10.1590/1519-6984.274368

Foam mat drying kinetics of jambolan and acerola mixed pulp
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Matos, Joana D’arc P. de

; Figueirêdo, Rossana M. F. de

; Queiroz, Alexandre J. de M.

; Moraes, Maria S. de

; Silva, Semirames do N.

; Silva, Luis P. F. R. da

Revista Brasileira de Engenharia Agrícola e Ambiental

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Jul 2022, Volume 26 Nº 7 Páginas 502 - 512

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A acerola e o jambolão são frutos com diversos compostos bioativos de origem fenólica e a composição de um produto elaborado com essas duas matérias-primas se afigura como um alimento com elevado potencial antioxidante e nutracêutico. Diante disso, este estudo foi realizado com o objetivo de avaliar a secagem em camada de espuma da polpa mista de jambolão e acerola. Foram elaboradas formulações com a polpa mista, contendo 1,0% de albumina com 0,5% de goma xantana (F1), 0,5% de carboximetilcelulose - CMC (F2) e 0,5% de goma guar (F3), desidratadas a 50, 60, 70 e 80 °C, com espessura da camada de espuma de 0,5 cm. A elevação da temperatura de secagem diminuiu o teor de água e tempo de desidratação das amostras. Os menores tempos de secagem foram obtidos na formulação F3; todos os modelos de secagem resultaram em bons ajustes, com destaque para Midilli. A maior difusividade efetiva foi obtida na formulação F3; a energia de ativação foi maior na combinação da F1. As amostras com albumina-guar (F3) apresentaram maiores entalpia e entropia e as com albumina-xantana (F1) as maiores energias livres de Gibbs. A secagem em camada de espuma é uma forma eficiente de conservação da polpa mista de jambolão e acerola.

ABSTRACT Acerola and jambolan are fruits with several bioactive compounds of phenolic origin and a product made with these two raw materials appears to be a food with high antioxidant and nutraceutical potential. Therefore, this study aimed to evaluate the foam mat drying of the mixed pulp of jambolan and acerola. Mixed pulp formulations, containing 1.0% albumin with 0.5% xanthan gum (F1), 0.5% carboxymethylcellulose (F2) and 0.5% guar gum (F3), were dehydrated at 50, 60, 70 and 80 °C, with foam mat thickness of 0.5 cm. The elevation of the drying temperature decreased the water content and dehydration time of the samples. Formulation F3 obtained the shortest drying times; all drying models resulted in good adjustments, especially Midilli. Formulation F3 obtained greater effective diffusivity; the activation energy was higher in the F1 combination. The samples with albumin-guar (F3) showed greater enthalpy and entropy and samples with albumin-xanthan gum (F1) showed the highest Gibbs free energy. Foam mat drying is an efficient way of preserving the jambolan and acerola mixed pulp.

https://doi.org/10.1590/1807-1929/agriambi.v26n7p502-512

Produção e efeito da germinação em sementes de jaca: caracterização química, física e físico-química
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Silva, Luís P. F. R. da

; Queiroz, Alexandre J. M.

; Figueirêdo, Rossana M. F.

; Campos, Ana Regina N.

; Matos, Joana D. P. de

; Moraes, Maria Suiane de

; Silva, Semirames do N.

; Gonçalves, Mailson G.

; Mascarenhas, Nágela M. H.

; Medeiros Neto, Moises S. de

; Rodrigues, Larissa M. S.

; Vieira, Agdylannah F.

; Costa, Zanelli R. T.

; Moura, Rodrigo L.

Revista de Ciências Agrárias

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Jun 2022, Volume 45 Nº 1-2 Páginas 51 - 60

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Jackfruit is an exotic fruit present in the Northeast region of Brazil, presenting a great economic potential for the region because of the internal and external commercialization of its fruit. Its seed is rich in carbohydrates, protein, minerals and vitamins, and is an alternative source of starch and for food. Germination is an easily accessible, inexpensive technology for the production of sprouts, improving the bioavailability of nutrients in the seed for consumption, such as proteins and bioactive compounds. The objective of this work was to evaluate the chemical, physical and physicochemical parameters of fresh and germinated jackfruit seeds. The germination of jackfruit seeds increased the contents of water, proteins, ascorbic acid, phenolic compounds and total sugars, in addition to reducing the content of starch, lipids, tannins and the parameters of color, luminosity, red (+ a*) and yellow (+b*) intensities. In relation to the physical properties of the seed, there was an increase in the real specific mass and in the unit mass. For apparent specific mass there was a reduction. Seed germination provided an increase in the levels of magnesium, iron and sodium, consequently reducing potassium, phosphorus, calcium, zinc, copper and manganese.

Resumo A jaca é um fruto exótico presente na região Nordeste do Brasil, apresentando um grande potencial econômico para região em face da comercialização interna e externa do seu fruto. Sua semente é rica em carboidratos, proteína, minerais e vitaminas, constituindo fonte alternativa de amido para alimentação. A germinação é uma tecnologia barata de fácil acesso para produção de brotos, melhorando a biodisponibilidade de nutrientes na semente para o consumo, a exemplo das proteínas e compostos bioativos. O objetivo do trabalho foi avaliar os parâmetros químicos, físicos e físico-químicos das sementes de jaca in natura e germinadas. A germinação das sementes de jaca proporcionou o aumento nos teores de água, proteínas, ácido ascórbico, compostos fenólicos e açúcares totais, além de reduzir os conteúdo de amido, lipídios, taninos e os parâmetros de cor, a luminosidade, intensidade de vermelho (+a*) e de amarelo (+b*). Em relação às propriedades físicas da semente, ocorreu acréscimo na massa específica real e na massa unitária. Para massa específica aparente houve redução. A germinação das sementes proporcionou aumento nos teores de magnésio, ferro e sódio, consequentemente ocorrendo redução de potássio, fósforo, cálcio, zinco, cobre e manganês.

https://doi.org/10.19084/rca.25988

COMPUTATIONAL INTELLIGENCE APPLIED IN OPTIMAL DESIGN OF WOODEN PLANE TRUSSES
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Christoforo, André L.

; Moraes, Matheus H. M. de

; Fraga, Iuri F.

; Pereira Junior, Wanderlei M.

; Lahr, Francisco A. R.

Engenharia Agrícola

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2022, Volume 42 Nº spe elocation e20210123

Resumo: En | Texto: En | PDF: En

ABSTRACT The use of wood is widespread in rural constructions, and the truss systems stand out among its various applications. This specific system has several typologies and requires a thorough study to determine the most advantageous model for each project. The present study aims to apply the Computational Intelligence concepts to determine the minimum viable cross-section of a Howe truss. For the computational simulation, methods like Finite Elements were used to obtain the loads and the Firefly Algorithm for the optimization process, focusing on minimizing the total weight of the structural part. Studies were conducted varying the spans of the elements and the height to span ratio. The design assumptions for establishing the optimization method’s constraints follow the recommendations of the Brazilian standard for wood design, ABNT NBR 7190 (1997). Weights between 95.42 kg and 653.57 kg were obtained, and all optimization processes presented feasible solutions for the design constraints.

https://doi.org/10.1590/1809-4430-eng.agric.v42nepe20210123/2022

Gathering patients and rheumatologists' perceptions to improve outcomes in idiopathic inflammatory myopathies
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Bertoglio, Isabela M.

; Abrahao, Glaucia F.

; de Souza, Fernando H.C.

; Miossi, Renata

; de Moraes, Paloma C.

; Shinjo, Samuel K.

; Bonfá, Eloisa

; Lopes, Michelle R. Ugolini

Clinics

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2022, Volume 77 elocation 100031

Resumo: En | Texto: En | PDF: En

Abstract Objective Therapeutic targets in Idiopathic Inflammatory Myopathies (IIM) are based on the opinions of physicians/specialists, which may not reflect the main concerns of patients. The authors, therefore, assessed the outcome concerns of patients with IIM and compared them with the concerns of rheumatologists in order to develop an IIM outcome standard set. Methods Ninety-three IIM patients, 51 rheumatologists, and one physiotherapist were invited to participate. An open questionnaire was initially applied. The top 10 answers were selected and applied in a multiple-choice questionnaire, inquiring about the top 3 major concerns. Answers were compared, and the agreement rate was calculated. Concerns were gathered in an IIM outcome standard set with validated measures. Results The top three outcome concerns raised by patients were medication side effects/muscle weakness/prevention functionality loss. The top three concerns among rheumatologists were to prevent loss of functionality/to ensure the quality of life/to achieve disease remission. Other's outcomes concerns only pointed out by patients were muscle pain/diffuse pain/skin lesions/fatigue. The agreement rate between both groups was 41%. Assessment of these parameters guided the development of an IIM standard set which included Myositis Disease Activity Assessment Visual Analogue Scale/Manual Muscle Testing/fatigue and pain Global Visual Analogue Scale/Health Assessment Questionnaire/level of physical activity. Conclusion The authors propose a novel standard set to be pursued in IIM routine follow-up, which includes not only the main patients/rheumatologist outcome concerns but also additional important outcomes only indicated by patients. Future studies are necessary to confirm if this comprehensive approach will result in improved adherence and ultimately in better assistance.

https://doi.org/10.1016/j.clinsp.2022.100031

10.

Antinociceptive and anti-inflammatory effects of extract of Celtis iguanaea (Jacq.) Sargent leaves in mice
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MORAES, WEULLER FILHO DE

; SILVA, DAIANY PRISCILLA B. DA

; FLORENTINO, IZIARA F.

; ALMEIDA, DIONYS S.

; MOREIRA, LORRANE KELLE S.

; NASCIMENTO, MARCUS VINÍCIUS M.

; CARVALHO, PABLINNY M.G. DE

; COUTO, RENÊ O. DO

; PAULA, JOSÉ R. DE

; COSTA, ELSON A.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20191339

Resumo: En | Texto: En | PDF: En

Abstract The antinociceptive and anti-inflammatory activities of crude ethanolic extract of Celtis iguanaea leaves and their active fractions are reported. The oral treatment with crude ethanolic extract (CEE; 100, 300 or 1000 mg/Kg) inhibited the number of writhings in a dose-dependent manner. The intermediate dose also inhibited formalin-induced nociception in both phases. The oral treatment with dichloromethane fraction (DF; 9 mg/Kg) produced antinociceptive effect in both phases of formalin test; however, the treatment with ethyl acetate fraction (EAF; 16 mg/Kg) reduced pain only in the second phase of this test. The oral treatments with CEE (300 mg/Kg) or DF (9 mg/Kg) reduced the nociception induced by capsaicin and pre-treatment with naloxone did not change these effects. The oral administration of CEE (300 mg/Kg), DF (9 mg/Kg) or ethyl EAF (16 mg/Kg) reduced ear edema, leukocytes migration and myeloperoxidase activity. Furthermore, the oral treatment with CEE (300 mg/Kg) or EAF (16 mg/Kg) reduced the level of Tumor Necrosis Factor - Alpha (TNF-α) in the pleurisy test. In conclusion, the DF showed antinociceptive activity that involves the vanilloid system as well as anti-inflammatory effect and the EAF showed anti-inflammatory activity involving the reduction of TNF-α cytokine.

https://doi.org/10.1590/0001-3765202220191339

11.

A first report of decapod crustaceans (Anomura and Brachyura) from Laje de Santos: a no-take marine reserve in the southeast coast of Brazil
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Moraes, Isabela R. R.

; Davanso, Thiago M.

; Silva, Alexandre R. da

; Cobo, Valter J.

; Alves, Douglas F. R.

; Santana, William

; Mantelatto, Fernando L.

; Castilho, Antonio L.

Revista mexicana de biodiversidad

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2022, Volume 93 elocation e933658

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Este estudio representa un esfuerzo sin precedentes para evaluar la diversidad de los decápodos Brachyura y Anomura del infralitoral rocoso del Parque Estatal Marino Laje de Santos, un área preservada y protegida localizada en la costa sureste de Brasil. Las muestras se tomaron trimestralmente durante 1 año con la combinación de sustrato de refugio artificial para captura pasiva y buceo autónomo para captura activa. Se determinaron un total de 987 individuos distribuidos en 32 especies, 22 géneros y 11 familias. Presentamos un reporte taxonómico de las especies y se discuten sus registros de distribución. El ermitaño Pagurus brevidactylus y el cangrejo Mithraculus forceps fueron las especies más abundantes de Anomura y Brachyura, respectivamente. Como se esperaba, la combinación de los métodos de muestreo mostró un valor más alto del índice de diversidad de Shannon (H = 5.7) que los métodos individuales de captura pasiva (H = 5.6) y captura activa (H = 4.4). Este estudio provee el primer reporte de crustáceos decápodos para el parque, por lo tanto, puede ser usado como línea base para programas de monitoreo de fauna local y en los planes de conservación.

Abstract This study represents an unprecedented effort to assess the diversity of rocky infralittoral decapods Brachyura and Anomura from the Marine State Park of Laje de Santos, a preserved and protected area located in the southeastern Brazilian coast. Sampling was carried out quarterly for 1 year, using the combination of artificial refuge substrate (ARS) for the passive capture of specimens, and SCUBA diving for active capture. A total of 987 individuals distributed into 32 species, 22 genera, and 11 families were determined. We present a taxonomic account of the species and their distribution records are discussed. The hermit crab Pagurus brevidactylus and the crab Mithraculus forceps were the most common species of Anomura and Brachyura, respectively. As expected, the combination of the sampling methodologies showed a higher Shannon diversity index value (H = 5.7), than the individual methods of ARS (H = 5.6) and active capture (H = 4.4). This study provides the first record of decapod crustaceans for the park, therefore it can be used as a baseline for local fauna monitoring programs and conservation planning.

https://doi.org/10.22201/ib.20078706e.2022.93.3658

12.

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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Dez 2021, Volume 36 Nº 6 Páginas 725 - 735

Resumo: En | Texto: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

13.

Allergic sensitization pattern of patients in Brazil
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Aranda, Carolina Sanchez

; Cocco, Renata R.

; Pierotti, Felipe F.

; Sarinho, Emanuel

; Sano, Flávio

; Porto, Arnaldo

; Rosário, Nelson

; Chong Neto, Herberto J.

; Goudouris, Ekaterini

; Moraes, Lillian S.

; Wandalsen, Neusa F.

; Mallozi, Marcia C.

; Pastorino, Antônio C.

; Franco, Jackeline M.

; Chavarria, Maria L.

; Borres, Magnus

; Solé, Dirceu

Jornal de Pediatria

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Ago 2021, Volume 97 Nº 4 Páginas 387 - 395

Resumo: En | Texto: En | PDF: En

Abstract Objective Allergic sensitization is one of the key components for the development of allergies. Polysensitization seems to be related to the persistence and severity of allergic diseases. Furthermore, allergic sensitization has a predictive role in the development of allergies. The aim of this study was to characterize the pattern of sensitization of atopic patients treated at different pediatric allergy referral centers in Brazil. Methods A nation-wide transversal multicenter study collected data on patients attended in Brazil. Peripheral blood samples were collected to determine the serum levels of allergen-specific IgE. If allergen-specific IgE was higher than 0.1 kUA/L, the following specific components were quantified. Results A total of 470 individuals were enrolled in the study. Mite sensitization was the most frequent kind in all participants. A high frequency of sensitization to furry animals and grasses featured in the respiratory allergies. Regarding components, there was a predominance of sensitization to Der p 1 and Der p 2. It has been verified that having a food allergy, atopic dermatitis, or multimorbidity are risk factors for the development of more severe allergic disease. Conclusion Studies on the pattern of allergic sensitization to a specific population offer tools for the more effectual prevention, diagnosis, and treatment of allergic diseases. Sensitization to dust mites house was the most prevalent in the evaluated sample. High rates of sensitization to furry animals also stand out. Patients with food allergy, atopic dermatitis, or multimorbidity appear to be at greater risk for developing more severe allergic diseases.

https://doi.org/10.1016/j.jped.2020.08.005 84 downloads

14.

Evaluation of the Seasonality and Extraction Method on the Polar Extracts of Croton grewioides Baill. by Chromatogram Fingerprinting and Isolation of a New Triglycosylated Flavonoid
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Prado, Vilma M. J.

; Jesus, Raphael A. de

; Oliveira, Julio M. A.

; Pereira, Camila S. A.

; Blank, Arie F.

; Pereira-Filho, Edenir R.

; Cass, Quezia B.

; Lima, Juliana M. de

; Ferreira, Antonio G.

; Nogueira, Paulo C. L.

; Moraes, Valéria R. S.

Journal of the Brazilian Chemical Society

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Fev 2021, Volume 32 Nº 2 Páginas 385 - 394

Resumo: En | Texto: En | PDF: En

Croton grewioides Baill. popularly known as “canelinha” or “canelinha-de-cheiro” has been used for the treatment of influenza, antitussive, febrifuge and headache; however, the study of its phytochemical composition is limited. The aim of this study was to investigate the effect of the extraction method and seasonality through leaf extracts of four accessions of Croton grewioides by fingerprint chromatograms aided by principal component analysis to analyze the differences and similarities among the samples. We aimed also to provide chemical characterization of isolated secondary metabolites using semi-preparative liquid chromatography. The results showed that only the chemical profile of the methanolic extracts of accessions 101 and 113 were influenced by the seasonality. For the first time, four flavonoids were isolated through semi-preparative chromatography in this species, characterized as quercetin 3-O-β-D-galactopyranosyl-(1→2)-α-L-rhamnopyranoside-(1→6)-α-L-rhamnopyranoside (1), quercetin 3-O-β-D-galactopyranosyl-(1→2)-α-apiopyranoside-(1→6)-α-L-rhamnopyranoside (2), quercetin 3-O-glucopyranoside (3) and 3-O-methyl-quercetin (4), the flavonoid (2) has been recognized as a new triglycosylated derivative.

https://doi.org/10.21577/0103-5053.20200190 249 downloads

15.

Email Vs. Instagram Recruitment Strategies For Online Survey Research
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Moraes, Rafael R.

; Correa, Marcos B.

; Daneris, Ândrea

; Queiroz, Ana B.

; Lopes, João P.

; Lima, Giana S.

; Cenci, Maximiliano S.

; D’Avila, Otávio P.

; Pannuti, Claudio M.

; Pereira-Cenci, Tatiana

; Demarco, Flávio F.

Brazilian Dental Journal

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Fev 2021, Volume 32 Nº 1 Páginas 67 - 77

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Neste estudo, descrevemos um método para atingir uma população-alvo (dentistas atuando no Brasil) para se engajar em pesquisa do tipo enquete (survey) utilizando convites tradicionais por e-mail e campanhas de recrutamento criadas no Instagram. Este estudo aborda aspectos metodológicos e compara as pessoas entrevistadas alcançadas por diferentes métodos. Foi utilizado um questionário pré-testado e os(as) participantes foram recrutados(as) por 10 dias por meio de uma lista de endereços de e-mail e duas campanhas abertas orgânicas independentes no Instagram. Foram coletadas 3122 respostas: 509 participantes foram recrutados(as) por e-mail (taxa de resposta 2,1%) e 2613 pelas duas campanhas do Instagram (taxas de conversão 20,7% e 11,7%), respectivamente. As taxas de coleta de resposta/min nas primeiras 24h variaram entre 0,23 (e-mail) e 1,09 (primeira campanha). No total, 98,8% das respostas foram recebidas nas primeiras 48h para as diferentes estratégias de recrutamento. Houve diferenças significativas para todas as variáveis demográficas (p<0,001) entre recrutados(as) por e-mail e Instagram, com exceção de sexo (p=0,37). As pessoas recrutadas via Instagram eram um pouco mais velhas, tinham mais experiência profissional (anos na prática) e nível de pós-graduação superior às entrevistadas por e-mail. Além disso, a maioria dos entrevistados por e-mail e Instagram trabalhava no setor público e na prática privada, respectivamente. Embora ambas estratégias tenham sido capazes de coletar respostas de todas as regiões brasileiras, as respostas por e-mail foram ligeiramente melhor distribuídas nas cinco áreas territoriais em comparação ao Instagram. Este estudo fornece evidências de que o recrutamento de uma amostra de população diversificada e grande usando o Instagram é viável. No entanto, a combinação de e-mail e Instagram no recrutamento levou a uma população mais diversificada e melhores taxas de resposta.

Abstract In this study, we describe a method for reaching a target population (i.e., dentists practicing in Brazil) to engage in survey research using traditional e-mail invites and recruitment campaigns created on Instagram. This study addresses methodological aspects and compares respondents reached by different methods. A pre-tested questionnaire was used and participants were recruited for 10 days via a source list of email addresses and two discrete Instagram organic open campaigns. A total of 3,122 responses were collected: 509 participants were recruited by email (2.1% response rate) and 2,613 by the two Instagram campaigns (20.7% and 11.7% conversion rates), respectively. Response/min collection rates in the first 24 h ranged between 0.23 (email) and 1.09 (first campaign). In total, 98.8% of all responses were received in the first 48 h for the different recruitment strategies. There were significant differences for all demographic variables (p< 0.001) between email and Instagram respondents, except for sex (p=0.37). Instagram respondents were slightly older, had more professional experience (years in practice), and a higher graduate education level than email respondents. Moreover, most email and Instagram respondents worked in the public sector and private practice, respectively. Although both strategies could collect responses from all Brazilian regions, email responses were slightly better distributed across the five territorial areas compared to Instagram. This study provides evidence that survey recruitment of a diverse, large population sample using Instagram is feasible. However, combination of email and Instagram recruitment led to a more diverse population and improved response rates.

https://doi.org/10.1590/0103-6440202104291 1314 downloads

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