ABSTRACT No-till farming is the practice closest to the concept of sustainable agriculture. The minimum soil movement and continuous contribution of crop residues to the farming system reduce erosion, mitigate the greenhouse effect, increase the organic matter content, and improve the physical and chemical quality of the soil. This study aimed to assess the effect of five-year succession cropping on the physical and chemical attributes of oxisol. The crops were sown for five consecutive years in the same plots, using a randomized block design in split plots with four replicates. The plots were crops grown in succession to soybean, namely Congo grass (Urochloa ruziziensis syn. Brachiaria ruziziensis), Congo grass intercropped with maize (Zea mays L.), pearl millet (Pennisetum glaucum), sorghum (Sorghum bicolor L.), maize, and slender leaf rattlebox (Crotalaria ochroleuca). The subplots were the following sampled soil layers: 0-5, 5-10, and 10-20 cm. The physicochemical attributes of these three soil layers were evaluated. Pearl millet cycled K efficiently, providing the soil with K concentrations equivalent to those of the K fertilization treatments. No single crop or intercrop increased the soil P concentration. Congo grass stood out for its ability to increase the Mg concentration. The 0-5 cm soil layer had the best physicochemical attributes based on the accumulated organic matter.

RESUMO O sistema plantio direto é o sistema que mais se aproxima do conceito de agricultura sustentável. A movimentação mínima do solo e o aporte contínuo de palhada ao sistema, reduz a erosão, contribui para a atenuação do efeito estufa, aumenta o teor de matéria orgânica e melhora a qualidade física e química do solo. Objetivou-se com este estudo avaliar o efeito de cinco anos de sucessão de culturas sobre os atributos físicos e químicos de latossolo vermelho distroférrico. As culturas foram semeadas por cinco anos consecutivos nas mesmas parcelas, utilizando o delineamento em blocos casualizados, em parcelas subdivididas, com quatro repetições. As parcelas foram cultivadas em sucessão à soja, sendo elas: braquiária solteira (Urocloa ruziziensis syn Brachiaria ruziziensis), braquiária consorciada com milho (Zea mays L.), milheto (Pennisetum glaucum), sorgo (Sorghum bicolor L.), milho e crotálaria (Crotalaria ochroleuca). Já as subparcelas, foram representadas pelas camadas de solo amostradas: 0-5, 5-10 e 10-20 cm. Foram avaliados os atributos químicos e físicos do solo, nas três camadas mencionadas. O milheto é eficiente na ciclagem de K, proporcionando níveis deste elemento no solo equivalentes aos tratamentos que receberam adubação potássica. Nenhuma cultura isolada ou em consórcio elevou a concentração de P no solo. A braquiária destaca-se pela sua capacidade de elevar as concentrações de Mg. A camada do solo de 0-5 cm possui as melhores condições físico-químicas em função da matéria orgânica acumulada.

RESUMO A salinização reduz o potencial osmótico da solução do solo e promove o acúmulo de íons tóxicos (Na+ e Cl-) nas plantas, causando desequilíbrio nutricional e reduções no rendimento. Assim, objetivou-se avaliar as concentrações foliares de nutrientes, Na+ e rendimento de colmos em cana-de-açúcar RB92579 sob diferentes valores de condutividade elétrica da água de irrigação e frações de lixiviação (FL). O experimento foi conduzido em lisímetros de drenagem em esquema fatorial 5 × 2: cinco condutividades elétricas da água de irrigação - CEa (0,5; 2,0; 4,0; 6,0 e 8,0 dS m-1) sem fração de lixiviação (FL1 = 0) e com (FL2 = 0,17), com quatro repetições. O aumento da CEa diminuiu as concentrações de N, P, K, Mg, S, Fe, Mn, Cu e Zn, e aumentou as concentrações de Ca, Cl e Na, e reduziu a produção de biomassa em dois ciclos (planta cana e soca). O uso da fração de lixiviação 0,17 mitigou os efeitos deletérios da salinidade nos nutrientes e na produtividade.

ABSTRACT Salinization reduces the osmotic potential of soil solutions and promotes the accumulation of toxic ions (Na+ and Cl-) in plants, causing nutritional imbalance and yield reductions. Thus, the objective of the present study was to evaluate foliar concentrations of nutrients and Na+ and stalk yields in sugarcane RB92579 under different electrical conductivities of irrigation water and leaching fractions (LF). The experiment was conducted in drainage lysimeters in a 5 × 2 factorial scheme with five electrical conductivities of irrigation water - ECw (0.5, 2.0, 4.0, 6.0, and 8.0 dS m-1) without (LF1 = 0) or with a leaching fraction (LF2 = 0.17), and four replicates. Increased ECw decreased the concentrations of N, P, K, Mg, S, Fe, Mn, Cu, and Zn and increased those of Ca, Cl, and Na, reducing the biomass production in two cycles (plant-cane and first ratoon). The use of a leaching fraction of 0.17 mitigated the deleterious effects of salinity on nutrient concentration and yield.

Resumo Shewanella xiamenensis G5-03 foi capaz de descolorir o corante azo vermelho Congo em água residuária sintética. A influência de alguns fatores na eficiência da descoloração do corante foi avaliada. As condições ótimas de descoloração foram temperatura de 30-35 °C, pH 10,0 e condições estáticas. A cinética de descoloração do vermelho Congo se ajustou ao modelo de Michaelis–Menten (Vmax = 111,11 mg L-1 h-1 and Km = 448,3 mg L-1). A bactéria também foi capaz de degradar a benzidina, um produto da quebra da ligação azo do vermelho Congo, o que contribuiu para a redução da fitotoxicidade. A habilidade da S. xiamenensis G5-03 em simultaneamente descolorir e degradar o vermelho Congo demostra seu potencial de aplicação no tratamento de águas residuárias contendo corantes azo.

Abstract Shewanella xiamenensis G5-03 was observed to decolorize the azo dye Congo red in synthetic wastewater. The influence of some factors on the dye decolorization efficiency was evaluated. The optimal decolorization conditions were temperature 30-35 °C, pH 10.0, incubation time 10 h, and static condition. The kinetic of Congo red decolorization fitted to the Michaelis–Menten model (Vmax = 111.11 mg L-1 h-1 and Km = 448.3 mg L-1). The bacterium was also able to degrade benzidine, a product of azo bond breakage of the Congo red, which contributed to reduce the phytotoxicity. The ability of S. xiamenensis G5-03 for simultaneous decolorization and degradation of Congo red shows its potential application for the biological treatment of wastewaters containing azo dyes.

Abstract Staphylococcus spp. represents the main mastitis agents in ruminants and contaminants of milk due to their expressive capacity to make biofilms. The aims in this study was evaluate evaluated the antimicrobial activity of Mauritia flexuosa L. extracts against Staphylococcus spp. adhered to a stainless steel surface. Two isolates from cows with clinical mastitis were evaluated; one was identified as Staphylococcus aureus, and the other Staphylococcus haemolyticus. Additionally the ATCC 25923 strain, S. aureus from human was evaluated. The chemical profile obtained from gas chromatography revealed the presence of carbohydrates, organic acids, and flavonoids. The minimum bactericidal concentrations of the ethanolic extract (EE) and aqueous extract (AE) were 4.4 and 5.82 mg/mL, respectively. After EE treatment at 4.4 mg/mL for 2.5 min, total removal of mature biofilms grown on stainless steel coupons was observed (reduction by 3.85-4.81 log units). This extract from M. flexuosa shows potential as an effective sanitizer and may represent a natural alternative against Staphylococcus spp.

Resumo Bactérias do gênero Staphylococcus spp. são os principais agentes da mastite em ruminantes e contaminantes do leite devido à expressiva capacidade de formação de biofilmes. Neste estudo o objetivo foi avaliar a atividade antimicrobiana de extratos de Mauritia flexuosa L. (Buritizeiro) contra Staphylococcus spp. aderidos à superfície de aço inoxidável. Foram avaliados dois isolados de vacas com mastite clínica; o um isolado foi identificado como Staphylococcus aureus e o outro como Staphylococcus haemolyticus. Adicionalmente foi também avaliada a e a cepa S. aureus ATCC 25923 de origem humana. O perfil químico obtido por cromatografia gasosa revelou a presença de carboidratos, ácidos orgânicos e flavonóides. As concentrações bactericidas mínimas do extrato etanólico (EE) e do extrato aquoso (AE) foram 4,4 e 5,82 mg / mL, respectivamente. Após o tratamento com EE a 4,4 mg / mL por 2,5 min, foi observada remoção total de biofilmes maduros cultivados em cupons de aço inoxidável (redução de 3,85-4,81 unidades log). O EE de folhas de M. flexuosa apresenta potencial como um desinfetante eficaz e pode representar uma alternativa natural contra Staphylococcus spp.

RESUMO A salinidade é um dos fatores que mais limita a produtividade agrícola no Semiárido brasileiro. Neste contexto, o presente estudo foi realizado com o objetivo de avaliar as trocas gasosas e as características biométricas de acessos do complexo Saccharum submetidas a estresse salino. O experimento foi realizado em casa de vegetação, instalada na sede da Embrapa Semiárido, em Petrolina, PE. O delineamento experimental foi em blocos casualizados, sendo os tratamentos representados por 19 acessos pertencentes a diferentes gêneros/espécies, sendo 10 acessos de Saccharum officinarum (BGCN 6, BGCN 91, BGCN 104, BGCN 127, BCGN 90, BGCN 101, BGCN 102, BGCN 118, BGCN 125 e BGCN 122), dois acessos de Saccharum spp. (BGCN 87 e BGCN 89), um acesso de Saccharum hybridum (BGCN 88), um acesso de Saccharum robustum (BGCN 94), quatro acessos de Erianthus arundinaceus (BGCN 117, BGCN 119, BGCN 120 e BGCN 123) e um acesso de Miscanthus spp., com três repetições. Foram avaliadas características biométricas, índice de clorofila e as trocas gasosas dos acessos submetidos à irrigação com água salinizada (6,0 dS m-1). Os acessos de E. arundinaceus (BGCN 120 e BGCN 123) apresentaram a maior taxa fotossintética, taxa de transpiração, altura de plantas e comprimento das folhas, indicando maior adaptabilidade ao estresse salino e podem ser promissores em programa de melhoramento.

ABSTRACT Salinity is one of the factors that most limit agricultural yield in the Brazilian semi-arid region. In this context, the present study aimed to evaluate the leaf gas exchange and biometric characteristics of accessions of the Saccharum complex subjected to salt stress. The experiment was carried out in a greenhouse, installed at Embrapa Semiárido, in Petrolina, PE, Brazil. The experimental design was in randomized blocks, with the treatments represented by 19 accessions belonging to different genera/species, being 10 accessions of Saccharum officinarum (BGCN 6, BGCN 91, BGCN 104, BGCN 127, BCGN 90, BGCN 101, BGCN 102, BGCN 118, BGCN 125 and BGCN 122), two accessions of Saccharum spp. (BGCN 87 and BGCN 89), one accession of Saccharum hybridum (BGCN 88), one accession of Saccharum robustum (BGCN 94), four accessions of Erianthus arundinaceus (BGCN 117, BGCN 119, BGCN 120 and BGCN 123) and one accession of Miscanthus spp., with three repetitions. Biometric characteristics, chlorophyll index and leaf gas exchange of the accessions were evaluated when they were subjected to irrigation with salinized water (6.0 dS m-1). E. arundinaceus accessions (BGCN 120 and BGCN 123) showed the highest photosynthetic rate, transpiration rate, plant height and leaf length, indicating greater adaptability to salt stress and could be promising in breeding programs.

RESUMO O conhecimento das relações hídricas e das respostas bioquímicas de plantas tem sido apontado como importante para a seleção de genótipos tolerantes aos estresses abióticos, tais como seca e salinidade. Assim, objetivou-se avaliar o potencial hídrico foliar, atividade enzimática e produtividade da cana-de-açúcar (RB 92579) irrigada com águas salobras e frações de lixiviação. A pesquisa foi conduzida no período de novembro 2016 a novembro 2017 em Recife, PE, Brasil. Adotou-se o delineamento experimental inteiramente casualizado, em esquema fatorial 5 x 2, com quatro repetições. Os tratamentos consistiram de cinco salinidades da água de irrigação (0,5; 2,0; 4,0; 6,0 e 8,0 dS m-1) e duas frações de lixiviação (0 e 0,17), correspondendo a 100 e 120% da evapotranspiração da cultura. Os valores de salinidade da água de irrigação foram obtidos pela adição de NaCl e CaCl2 na proporção de 1:1 molar (Ca:Na) na água de abastecimento (CEa = 0,5 dS m-1). Foram realizadas leituras do potencial hídrico foliar (Ψw) no antemanhã e coletadas folhas +3 para a determinação da atividade da catalase e da ascorbato peroxidase aos 90, 150, 210 e 270 dias após o plantio. A produtividade foi determinada aos 365 dias após o plantio. O aumento da salinidade da água reduziu o potencial hídrico foliar e o rendimento, e, aumentou a atividade da catalase e ascorbato peroxidase, sendo a fração de lixiviação de 0,17 capaz de reduzir os efeitos deletérios dos sais sobre as plantas.

ABSTRACT Knowledge on the water relations and biochemical responses of plants has been pointed out as important for selecting genotypes that are tolerant to abiotic stresses, such as drought and salinity. Thus, the objective of this study was to evaluate the leaf water potential, enzymatic activity and yield of sugarcane (RB 92579) irrigated with brackish waters and leaching fractions. The study was conducted from November 2016 to November 2017 in Recife, PE, Brazil. The experimental design was completely randomized, in a 5 x 2 factorial scheme, with four repetitions. Treatments consisted of five irrigation water salinity (0.5; 2.0; 4.0; 6.0 and 8.0 dS m-1) and two leaching fractions (0 and 0.17), corresponding to 100 and 120% of crop evapotranspiration. The values of irrigation water salinity were obtained by adding NaCl and CaCl2 at molar proportion of 1:1 (Ca:Na) to water from the local supply (ECw = 0.5 dS m-1). Readings of leaf water potential (Ψw) were taken at predawn, and +3 leaves were collected for the determination of catalase and ascorbate peroxidase activity at 90, 150, 210 and 270 days after planting. Yield was determined at 365 days after planting. Increase in water salinity reduced leaf water potential and yield, and increased the activity of catalase and ascorbate peroxidase, and the leaching fraction of 0.17 was able to reduce the deleterious effects of salts on plants.

Abstract The Nobel Prize in Chemistry 2019 recognized the importance of Li-ion batteries and the revolution they allowed to happen during the past three decades. They are part of a broader class of electrochemical energy storage devices, which are employed where electrical energy is needed on demand and so, the electrochemical energy is converted into electrical energy as required by the application. This opens a variety of possibilities on the utilization of energy storage devices, beyond the well-known mobile applications, assisting on the decarbonization of energy production and distribution. In this series of reviews in two parts, two main types of energy storage devices will be explored: electrochemical capacitors (part I) and rechargeable batteries (part II). More specifically, we will discuss about the materials used in each type of device, their main role in the energy storage process, their advantages and drawbacks and, especially, strategies to improve their performance. In the present part, electrochemical capacitors will be addressed. Their fundamental difference to batteries is explained considering the process at the electrode/electrolyte surface and the impact in performance. Materials used in electrochemical capacitors, including double layer capacitors and pseudocapacitive materials will be reviewed, highlighting the importance of electrolytes. As an important part of these strategies, synthetic routes for the production of nanoparticles will also be approached (part I).

Abstract In the second part of the review on electrochemical energy storage, the devolvement of batteries is explored. First, fundamental aspects of battery operation will be given, then, different materials and chemistry of rechargeable batteries will be explored, including each component of the cell. In negative electrodes, metallic, intercalation and transformation materials will be addressed. Examples are Li or Na metal batteries, graphite and other carbonaceous materials (such as graphene) for intercalation of metal-ions and transition metal oxides and silicon for transformation. In the positive electrode section, materials for intercalation and transformation will be reviewed. The state-of-the-art on intercalation as lithium cobalt oxide and nickel containing oxides will be approached for intercalation materials, whereas sulfur and metal-air will also be explored for transformation. Alongside, the role of electrolyte will be discussed concerning performance and safety, with examples for the next generation devices. Finally, a general future perspective will address both electrochemical capacitors and batteries.

The objective of this study was to explore the extraction and identification of volatile organic compounds (VOCs) present in cagaita fruit (Eugenia dysenterica). Parameters such as type of extraction fiber, agitation, extraction time and extraction temperature were investigated. Initially, the VOCs were extracted using headspace solid-phase microextraction. Then, the compounds were identified using gas chromatography coupled to mass spectrometry. Results revealed the presence of at least 26 different compounds and the polyacrylate (PA) fiber promoted the extraction of a larger number of VOCs. Regarding the PA fiber, the most efficient extraction was achieved using a 50 rpm agitation at 45 ºC for 30 min. The majority were monoterpenes (34.64%) and esters (36.28%). An increase in the extraction temperature promoted the isolation of more VOCs when using carboxen/polydimethylsiloxane (CAR/PDMS) and divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibers. However, these fibers allowed the isolation of a smaller number of VOCs. The extraction time and agitation had no significant influence.

The use of insects to identify drugs in a cadaver has often been explored in the field of entomotoxicology. There are accurate methods; however, they require a high cost and are very time-consuming. The objective of this study was to develop two methods based on differential pulse voltammetry (DPV) and fluorescence excitation-emission matrix (EEM) spectroscopy to classify necrophagous larvae (Chrysomya megacephala, C. albiceps, Lucilia sp. and Cochliomyia macellaria) containing flunitrazepam. The voltammograms of larval extract samples were analyzed by principal component analysis (PCA), successive projection algorithm (SPA) and genetic algorithm (GA): linear and quadratic discriminant analysis (LDA and QDA). The EEM fluorescence data from larval extract samples were analyzed by 2D and parallel factor analysis (PARAFAC) with LDA. These results suggest that DPV and EEM combined with chemometrics can be used as tools for the classification of flunitrazepam in fly larvae presenting innovative applications.

Resumo Objetivo A obesidade durante a gestação é um dos fatores de risco mais estabelecidos para uma programação negativa em longo prazo. O objetivo do presente estudo foi investigar os efeitos do consumo materno de uma dieta hiperlipídica durante a gestação e lactação no aumento do peso, do tecido adiposo visceral e colesterolemia em ratos neonatos. Métodos Ratos Wistar foram divididos em dois grupos de acordo com a dieta da mãe durante a gestação e lactação: grupo controle (GC, n=12) composto por filhotes de ratas alimentadas com uma dieta padrão (lipídios 4%) e o grupo teste (GT, n=12) composto de filhotes de ratas alimentadas com dieta hiperlipídica (lipídios 23%). O peso dos animais foi aferido em dias alternados até o 22° dia de vida, quando foi coletado sangue para análises bioquímicas. Para a análise estatística utilizou-se os seguintes testes: two way ANOVA, teste de Sidak e teste t de Student, com p< 0,05. Resultados O grupo teste mostrou diferença no ganho de peso, no tecido adiposo visceral e nos níveis de colesterol. Conclusão Uma exposição materna a uma dieta hiperlipídica durante a gestação e lactação pode promover maior ganho ponderal, hipercolesterolemia e um aumento do tecido adiposo em ratos neonatos.

Abstract Aim Obesity during pregnancy is one of the most established risk factors for negative long-term programming. The aim of the present study was to investigate the effects of maternal consumption of a high-fat diet during pregnancy and lactation on the weight gain, visceral adipose tissue and cholesterolemia in neonatal rats. Methods Wistar rats were divided into two groups according to the mother's diet during pregnancy and lactation: Control group (CG, n = 12) were the offspring of rats fed a standard diet (4% lipid) and the Test group (TG, n = 12) were pups rats fed on a high fat diet (23% lipid). The weight of the animals was measured on alternate days until the 22nd day of life, when collected visceral adipose tissue and blood were collected for biochemical analysis. For statistical analysis the Student t test, Sidak´s teste and two way ANOVA was used, with p <0.05. Results the test group showed differences in weight gain, visceral adipose tissue and higher cholesterol. Conclusion a maternal exposure to a high-fat diet during pregnancy and lactation can promote changes in weight gain, hypercholesterolemia and an increase in adipose tissue in neonatal rats.

Resumo Este estudo objetivou avaliar a atividade antibacteriana in vitro dos fitoquímicos timol, linalol e citronelol sobre Streptococcus mutans, Streptococcus salivaris e Streptococcus oralis. Utilizou-se a técnica de discos de difusão em meio sólido e medição do diâmetro dos halos de inibição. A concentração inibitória mínima (CIM) das substâncias foi determinada utilizando diluições em série das substâncias e técnica de microdiluição em meio de cultura de Brain Heart Infusion. Após incubação durante 24 horas em estufa a 37 °C, a leitura da placa foi confirmada pelo método visual usando o corante 2,3,5 trifenil cloreto de tetrazólio. A concentração bactericida mínima (CBM) foi determinada a partir de subculturas de MIC. Os ensaios foram realizados em triplicata, e clorexidina foi usada como um controle positivo. Os diâmetros dos halos de inibição do crescimento variaram entre 7,3 e 10,7 por S. mutans, 7,3 e 10,0 por S. oralis, e 8,2 e 9,8 para S. salivaris. Os valores de CIM e CBM obtidos variaram de valores máximos na presença de linalol (1.250,0 mg/mL, 2.500.0 mg/mL e 2.500.0 mg/mL, respectivamente, para o S. mutans, S oralis e S. salivaris); a valores mínimos com timol (312,5 μg/ml, 156,2 μg/mL e 156,2 μg/ml, respectivamente para S. mutans, S. oralis e S. salivaris). Todos os fitoquímicos testados apresentaram atividade antibacteriana, representando, assim, substâncias com potencial de aplicações na prevenção da cárie dentária.

Abstract This study aimed to evaluate the in vitro antibacterial activity of the phytochemicals thymol, linalool, and citronellol against Streptococcus mutans, Streptococcus salivarius and Streptococcus oralis. Disk diffusion screening on solid medium and measurement of the diameter of the bacterial growth inhibition halos was the technique utilized. The Minimum Inhibitory Concentration (MIC) of the substances was determined using serial substance dilutions and microdilution technique in Brain Heart Infusion culture medium. After incubation for 24 hours in an oven at 37 °C, plate reading was completed and confirmed by visual method using 2,3,5 triphenyl tetrazolium chloride dye. The Minimum Bactericidal Concentration (MBC) was determined from MIC subcultures. Assays were performed in triplicate, and chlorhexidine was used as a positive control. The diameters in mm of the growth inhibition halos ranged between 7.3 and 10.7 for S. mutans, 7.3 and 10.0 for S. oralis, and 8.2 and 9.8 for S. salivarius. The MIC and MBC values obtained converged, ranging from maximum values in the presence of Linalool (1,250.0 mg/mL, 2,500.0 mg/mL and 2,500.0 mg/mL, respectively, for S. mutans, S. oralis, and S. salivarius); and minimum values with Thymol (312.5 μg/ml, 156.2 μg/mL and 156.2 μg/ml, respectively for S. mutans, S. oralis, and S. salivarius). All the tested phytochemicals displayed antibacterial activity, thus representing substances with potential applications in preventing tooth decay.

Mass spectrometry (MS) is a powerful technique that can provide the biochemical signature of a wide range of biological materials such as cells and biofluids. However, MS data usually has a large range of variables which may lead to difficulties in discriminatory analysis and may require high computational cost. In this paper, principal component analysis with linear discriminant analysis (PCA-LDA) and quadratic discriminant analysis (PCA-QDA) were applied for discrimination between healthy control and cancer samples (ovarian and prostate cancer) based on MS data sets. In addition, an identification of prostate cancer subtypes was performed. The results obtained herein were very satisfactory, especially for PCA-QDA. Selectivity and specificity were found in a range of 90-100%, being equal or superior to support vector machines (SVM)-based algorithms. These techniques provided reliable identification of cancer samples which may lead to fast and less-invasive clinical procedures.

The use of digital image analysis as an analytical tool is a reality nowadays, and the use of smartphones stands out due to its high accessibility and practicality. Ascorbic acid (AA) is a natural and essential vitamin available as a supplement as a result of its use in preventing and treating several pathologies. This paper reports a simple, fast and low cost method using smartphone image analysis for quantification of AA based on its interfering effect in the enzymatic colorimetric detection of glucose. Commercial vitamin C tablets were used as prediction set for AA quantification, showing very satisfactory results (relative errors < 4%), where no statistical difference at a 95% confidence level was observed between the AA content estimated by the imaging method and the labeled reference values. As advantages, this method does not use expensive reagents neither laborious procedures to carry out the analysis.

This paper presents a low-cost reflectometer for selective detection of Cu2+. The reflectometer is based on an emission light source using five light-emitting diodes at different wavelengths and a detector using a webcam. The samples were prepared using a filter paper-based colorimetric sensor with ascorbic acid-based quinoxaline derivative. Video analysis using gray color intensity was used to perform both copper ion screening (Cu2+, Pb2+, Cr+3, Ni2+, Fe2+, Sn2+, Mg2+, Cd2+, Ag+ and Co2+) and quantification of Cu2+ in spiked samples. In addition, a multivariate validation was performed. The system proved to be selective to Cu2+ among the screened ions, and its quantification was performed using partial least square regressions. Good linearity (R2 = 0.979, coefficient of determination), low root-mean-square error of prediction (RMSEP = 5.20 × 10-3 mol L-1) and high recovery (93.39-128.64%) were achieved. This method has potential to be employed for rapid and selective determination of Cu2+ in water using low-cost instrumentation.