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ABSTRACT Using nematode-resistant and/or -tolerant rootstock is a strategy that has maintained guava cultivation feasible in the irrigated perimeters of the Brazilian semi-arid region. The objective was to evaluate the production, gas exchange, and fruit quality of ‘Paluma’ guava with and without nematode-tolerant rootstock under different irrigation depths and production periods. The experimental design was randomized blocks, in the mixed 2 × 4 × 2 factorial scheme, corresponding to two forms of cultivation of ‘Paluma’ guava (without and with nematode-tolerant rootstock), four irrigation depths (60, 80, 100, and 120% of crop evapotranspiration - ETc), and two production periods, with four replicates. Guava plants were evaluated for physiological, production and post-harvest quality parameters. Gas exchange in ‘Paluma’ guava leaves was higher in the second production cycle, under irrigation depths above 100% ETc, not differing with the use of the rootstock. The use of the rootstock ‘BRS Guaraçá’ promotes a greater number of fruits and production with the increase of the irrigation depth, mainly in the second half of the year, with the increase of soluble solids and titratable acidity in the fruits.

RESUMO A utilização de porta enxerto resistente e ou tolerante a nematoides é uma estratégia que tem tornado viável a manutenção dos cultivos de goiabeira nos perímetros irrigados do semiárido brasileiro. Assim, o objetivo foi avaliar a produção, trocas gasosas e qualidade dos frutos da goiabeira ‘Paluma’ com e sem o porta enxerto tolerante a nematoide sob diferentes lâminas de irrigação e épocas de produção no Semiárido Brasileiro. O delineamento experimental foi em blocos ao acaso, no esquema fatorial misto 2 × 4 × 2, sendo duas formas de cultivo da goiabeira ‘Paluma’ (sem e com o porta enxerto tolerante a nematoide), quatro lâminas de irrigação (60, 80, 100 e 120% da evapotranspiração da cultura - ETc) e duas épocas de produção, com quatro repetições. Foram realizadas avaliações fisiológicas das plantas, produção e qualidade pós-colheita. As trocas gasosas nas folhas da goiabeira ‘Paluma’ foram maiores no segundo ciclo de produção, quando irrigada com lâminas de irrigação acima de 100% da ETc, não diferindo com o uso do porta enxerto. O uso do porta-enxerto ‘BRS Guaraçá’ proporciona um maior número de frutos e produção com o aumento da lâmina de irrigação, principalmente no segundo semestre do ano, bem como o aumento do teor de sólidos solúveis e acidez nos frutos.

https://doi.org/10.1590/1807-1929/agriambi.v27n5p400-406

Cut off Behavior of Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) in Soybean (Glycine max (L.) Merrill) Seedlings
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Araújo, Waldenio Antonio de

; Degrande, Paulo Eduardo

; Malaquias, José Bruno

; Silvie, Pierre Jean

; Scoton, Ana Maria Nascimento

; Pachú, Jéssica Karina da Silva

Brazilian Archives of Biology and Technology

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2023, Volume 66 elocation e23220386

Resumo: En | Texto: En | PDF: En

Abstract In addition to destroying the leaves, stems, pods, and grains of soybean (Glycine max), Spodoptera frugiperda larvae may also have the cut off behavior in the seedlings close to the ground, harming the establishment of the soybean crop. Therefore, this study aimed to evaluate the consequences of the fall armyworm attack by adopting the cut off behavior, which has been similarly documented for black cutworm (Agrotis ipsilon) in newly emerged soybean plants. The treatments were five levels of infestation with 0, 5, 10, 20 and 40 larvae per m2. Three variables were assessed: (1) stand (relationship between attacked and initial number of plants), (2) types and amounts of injured structures, such as hypocotyl (embryonic shoot), and the cotyledons (seed leaves), and (3) level of defoliation. The variables were collected every 24 hours until the pupal stage. Additionally, the final stand of the seedlings was quantified, with the highest injury intensity observed in the plots that contained the highest number of larvae. In the second experiment, the dynamics of movement and attacks of plants by S. frugiperda larvae at the following times of the day: 07:00, 10:00, 13:00, 16:00, 19:00 and 21:00 were analyzed with supervised machine learning models. The injury caused by S. frugiperda with the behavior of the cut off and the voracity of the larvae were evident in the structures (hypocotyl and cotyledons) of the evaluated plants. The results of the present study emphasize the need to manage S. frugiperda during the preplanting phase of soybean.

https://doi.org/10.1590/1678-4324-2023220386

Healthcare workers exposed to COVID-19 patients present an inflammatory status and Th2/Th17/Th22 immune profile: findings from before vaccine application in Brazil
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AQUINO, RODRIGO CESAR A. DE

; BARROS, BÁRBARA RAFAELA S.

; SILVA, GUILHERME ANTONIO S.

; SOUSA, GEORON F. DE

; SOUZA, EDSON B. DE

; SILVA, DYEGO R.C.

; NASCIMENTO, ARIONE V. DO

; SÁ, IGOR W.A. DE

; LIMA, ELKER L.S. DE

; SILVA, BÁRBARA O.

; LIMA, LUÍSA P.O. DE

; VIEIRA, AMANDA M.

; BARBOSA NETO, ADAUTO G.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; OLIVEIRA, TALITA HELENA ARAÚJO DE

; CARVALHO, BRUNO M.

; MUNIZ, MARIA TEREZA C.

; FREITAS, ANTONIO CARLOS DE

; CAMPELO JÚNIOR, EVÔNIO B.

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220502

Resumo: En | Texto: En | PDF: En

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

O AMBIENTE ESTUARINO E A VARIAÇÃO DE pH: LIMITES NATURAIS E OBSERVAÇÃO EXPERIMENTAL DO EFEITO DA ACIDIFICAÇÃO SOBRE A BIODISPONIBILIDADE DE FÓSFORO
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Braga, Elisabete S.

; Lucena, Luanny M.

; Almeida, Antonio Jeferson M.

; Pires, Maria Luiza T.

; Nascimento, José Elias F. do

; Sutti, Bruno O.

; Berbel, Glaucia B. B.

; Chiozzini, Vitor G.

Química Nova

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2023, Volume 46 Nº 6 Páginas 591 - 607

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

THE ESTUARINE ENVIRONMENT AND pH VARIATION: NATURAL LIMTS AND EXPERIMENTAL OBSERVATION OF THE ACIDIFICATION EFFECT ON PHOSPHORUS BIOAVAILABILITY. This study shows the variation of pH in the Cananéia-Iguape Estuarine-Lagoon Complex (CIELC). Data from 3 years (2019, 2021, 2022) were obtained in 17 points presenting the following ranges: temperature (14.88-27.05 ºC), pH (7.16-8.40) and DIP (0.20-11.28 µmol L-1) along a saline gradient (0.05-32.09) under different hydrodynamics, biogeochemical processes and anthropogenic influence. The pH buffering capacity due to the presence of weak acid salts in saline water (S ≥ 30) was associated to the lowest DIP, decreasing with low salinity values, confirming the direct correlation among salinity and pH. The highest temperatures in the winter of 2021, corroborated with the abnormal climate event in that year. An in vitro experiment showed results of the interaction of PID and sediments with different textures, with and without the presence of the benthic microbiota under a considerable decreasing of the pH (acidification) in relation to the natural condition of this environment. The P sediment flux characterized Iguape sector as a P sink with or without biota, Ararapira sector as a P source with biota and Cananéia, as P source without biota. The salt water buffered the pH and sediment buffered DIP both associated to the biogeochemical and hydrodynamic processes contribute to the homeostasis in the system.

https://doi.org/10.21577/0100-4042.20230054

Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
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SOUZA-SILVA, GUILHERME ANTONIO DE

; AQUINO, RODRIGO CESAR A. DE

; SOUSA, GEORON F. DE

; BARROS, BÁRBARA RAFAELA S.

; LIMA, MARÍLLIA RAPHAELLA C.F. DE

; CRUZ, LEONARDO C.O.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; TALES, APARECIDA VIRGÍNIA S.

; DINIZ, MADI V.

; LIMA, MÔNICA MARIA C.

; SÁ, IGOR W.A. DE

; RODRIGUES, FÁBIO AUGUSTO C.

; SOUZA, EDSON B. DE

; NASCIMENTO, ARIONE V. DO

; VIEIRA, AMANDA M.

; CARVALHO, BRUNO M. DE

; CAMPELO JÚNIOR, EVÔNIO B.

; FREITAS, ANTONIO CARLOS DE

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 3 elocation e20211590

Resumo: En | Texto: En | PDF: En

Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

https://doi.org/10.1590/0001-3765202220211590

Samanea tubulosa Benth. (Fabaceae): Antinociceptive effect on acute pain in mice: K+ATP channel and opioid activity
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ALIXANDRE, TAMNATA F.

; SOUSA, RENATO P.

; GOMES, BRUNO S.

; SILVA, ARÊTHA H.S.

; SOUSA NETO, BENEDITO P.

; SOUSA, ELCILENE A.

; LIMA, MARLUCE P.D.

; LOPES, EVERTON M.

; PIAUILINO, CELYANE A.

; NASCIMENTO, REJANE T.

; REIS FILHO, ANTÔNIO C.

; ALMEIDA, FERNANDA R.C.

; OLIVEIRA, FRANCISCO A.

; CHAVES, MARIANA H.

; COSTA, LUCIANA M.

; ALVES, MICHEL M. MORAES

; COSTA, AMILTON P.R.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 2 elocation e20210715

Resumo: En | Texto: En | PDF: En

Abstract Samanea tubulosa Benth. it has been widely used in traditional medicine to treat inflammatory processes. The present study aimed to investigate the antinociceptive effect and mechanism of action of the fractions obtained from the Samanea tubulosa pods in mice. The antinociceptive activity was evaluated in formalin, capsaicin and glutamate tests and the. The possible mechanisms of action involved in the antinociceptive effect of the hexane and ethyl acetate fraction in the opioid system, also the the K + ATP channels and the L-arigine pathways of nitric oxide were evaluated. The chemical characterization analysis revealed in the hexane fraction the presence of triterpenes such as lupenone and lupeol. In the glutamate test, the hexane and ethyl acetate fractions showed antinociceptive activity at the dose of 12.5 and 25 mg kg-1. The antinociception produced by the hexane and ethyl acetate fractions was significantly reversed by naloxone, indicating that the fractions act through the opioid pathway. Antinociceptive response of the ethyl acetate fraction was blocked by glibenclamide, indicating that this fraction acts via the K + ATP channels activation. It is concluded that the fractions under study exert antinociceptive activity possibly related to the opioid route and through K+ ATP channels activation.

https://doi.org/10.1590/0001-3765202220210175

Proteolytic activity of Triatoma infestans saliva associated with PAR-2 activation and vasodilation
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Oliveira, Karla A.

; Torquato, Ricardo J. S.

; Lustosa, Daniela C. G. Garcia

; Ribeiro, Tales

; Nascimento, Bruno W. L.

; Oliveira, Lilian C. G. de

; Juliano, Maria A.

; Paschoalin, Thaysa

; Lemos, Virginia S.

; Araujo, Ricardo N.

; Pereira, Marcos H.

; Tanaka, Aparecida S.

Journal of Venomous Animals and Toxins including Tropical Diseases

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2021, Volume 27 elocation e20200098

Resumo: En | Texto: En | PDF: En

ABSTRACT Background Triatoma infestans (Hemiptera: Reduviidae) is a hematophagous insect and the main vector of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae). In the present study, the authors investigated whether a serine protease activity from the saliva of T. infestans has a role in vasomotor modulation, and in the insect-blood feeding by cleaving and activating protease-activated receptors (PARs). Methods T. infestans saliva was chromatographed as previously reported for purification of triapsin, a serine protease. The cleavage activity of triapsin on PAR peptides was investigated based on FRET technology. Mass spectrometry was used to analyze the sites of PAR-2 peptide cleaved by triapsin. NO measurements were performed using the DAN assay (2,3-diaminonapthalene). The vasorelaxant activity of triapsin was measured in vessels with or without functional endothelium pre-contracted with phenylephrine (3 µM). Intravital microscopy was used to assess the effect of triapsin on mouse skin microcirculation. Results Triapsin was able to induce hydrolysis of PAR peptides and showed a higher preference for cleavage of the PAR-2 peptide. Analysis by mass spectrometry confirmed a single cleavage site, which corresponds to the activation site of the PAR-2 receptor. Triapsin induced dose-dependent NO release in cultured human umbilical vein endothelial cells (HUVECs), reaching a maximum effect at 17.58 nM. Triapsin purified by gel-filtration chromatography (10-16 to 10-9 M) was applied cumulatively to mouse mesenteric artery rings and showed a potent endothelium-dependent vasodilator effect (EC30 = 10-12 M). Nitric oxide seems to be partially responsible for this vasodilator effect because L-NAME (L-NG-nitroarginine methyl ester 300 µM), a nitric oxide synthetase inhibitor, did not abrogate the vasodilation activated by triapsin. Anti-PAR-2 antibody completely inhibited vasodilation observed in the presence of triapsin activity. Triapsin activity also induced an increase in the mouse ear venular diameter. Conclusion Data from this study suggest a plausible association between triapsin activity mediated PAR-2 activation and vasodilation caused by T. infestans saliva.

https://doi.org/10.1590/1678-9199-jvatitd-2020-0098 533 downloads

Influence of creep feeder position on the behavior and performance of preweaning piglets and sows in a hot climate environment
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OLIVEIRA, EWERSON S.

; NASCIMENTO, ELAINE L.L.

; LIMA, HELLEN K.S.

; NEVES, JACQUELINE S.

; DAMASCENO, JAMILLY G.

; SILVA, JULIANA C.

; OLIVEIRA, NATÁLIA C.

; NASCIMENTO, PEDRO H.

; OLIVEIRA, RAFAEL A.

; ARAÚJO, VITOR O.

; VIEIRA, MARIA F.A.

; MONTEIRO, BRUNO M.

; SCHINCKEL, ALLAN P.

; GARBOSSA, CESAR A.P.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 Nº 3 elocation e20200248

Resumo: En | Texto: En | PDF: En

Abstract The use of creep feeding for preweaning piglets is important to improve the performance of the piglets. The objective of this experiment was evaluate the effect of using or altering the position of piglet’s creep feeder during lactation on piglet’s performance and on behavior of piglets and sows kept in a hot climate environment. Forty-five sows and their litters at 10 days of lactation were randomly distributed into three treatments: front feeder (FF) - near the side of the sow’s head; back feeder (BF) - near the side of the rump of the sow; and no feeder (NF). All piglets were weighed individually to evaluate the average weight, weight gain and coefficient of variation of the weight. Behavior assessments of the piglets and sows were recorded in 3 period. At 15 and 21 d, piglets of the FF treatment were heavier (P ≤ 0.0001) than piglets of the other treatments. At 10-21d piglets of FF treatment had 76.2% less belly nosing behavior than the NF piglets (P=0.015). The treatments had no impact on behavior of the sows. The creep feeders positioned in the front of the farrowing crate increased piglet growth rate and decreased frequency of belly nosing behavior.

https://doi.org/10.1590/0001-3765202120200248 368 downloads

Evaluation of the Synthesis of Zeolite SSZ-13 using Choline Chloride and Different Crystallization Seeds and its Application in the Thermocatalytic Degradation of Ultra-High Molecular Weight Polyethylene
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Solano, Julyane R. S.

; Nascimento, Raul C. S.

; Silva, David C. M.

; Silva, Diogo P. S.

; Sousa, Lenivaldo V.

; Silva, Bruno J. B.

; Alencar, Soraya L.

; Urbina, Maritza M.

; Quintela, Paulo H. L.

; Silva, Antonio O. S.

Materials Research

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2021, Volume 24 Nº 5 elocation e20210066

Resumo: En | Texto: En | PDF: En

Abstract The synthesis of SSZ-13 was studied by evaluating the influence of two types of crystallization seeds with CHA structure (S1 seeds prepared in fluoride medium and S2 seeds by the conversion of zeolite Y), SiO2/Al2O3 (28, 40, 60 and 100) and OH/SiO2 (0.6 and 0.8) ratios. Choline chloride was used as the structure-directing agent and the crystallizations occurred at 140 ºC from 24 to 96 h. S1 seeds led to the coexistence of zeolites SSZ-13 and P, while S2 seeds promoted in all SiO2/Al2O3 ratios and OH/SiO2 = 0.8, the formation of pure SSZ-13 after 24 h. The SSZ-13 samples are formed by clusters of crystals with cuboid-like morphology and exhibited textural properties typical of CHA framework. SSZ-13 zeolite led to a decrease in the temperature and the activation energy required for the degradation of ultra-high molecular weight polyethylene polymer, demonstrating its efficiency as catalyst in this process.

https://doi.org/10.1590/1980-5373-mr-2021-0066 152 downloads

10.

Distribution of Siluriformes in a river under the influence of a small hydroelectric power plant of the Paraná River Basin, Brazil
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Garcia, Thiago D.

; Ota, Renata R.

; Ferreira, Dhiego G.

; Nascimento, Raul H. C.

; Galindo, Bruno A.

; Pereira, Larissa S.

; Zanatta, Augusto S.

Iheringia. Série Zoologia

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2020, Volume 110 elocation e2020005

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Quando uma barragem é construída, seus impactos diferem de acordo com a distância relativa da barragem, sendo que os locais mais próximos da barragem são mais afetados que os mais distantes. O barramento de um rio resulta em modificações no fluxo do rio e características da paisagem, modificando sua dinâmica hidrológica, limnológica e ecológica, como produção biológica, distribuição de espécies e estruturação do ecossistema e suas funções. O padrão de distribuição de Siluriformes (bagres) no rio Jaguariaíva foi avaliado, considerando três distâncias da barragem: montante, reservatório e jusante. As amostragens foram realizadas em três regiões distintas do rio Jaguariaíva considerando a distância em relação à barragem, entre março de 2013 e dezembro de 2014, com redes de espera, e as distintas zonas originadas pelo represamento do rio. A abundância e riqueza de espécies foram avaliadas. Um total de 840 indivíduos de Siluriformes foi capturado, distribuídos em dez espécies e quatro famílias. A maior riqueza foi observada na região a jusante do reservatório, enquanto a menor foi verificada na região da barragem. Diferenças significativas na abundância das espécies entre as três regiões foram observadas; a região a jusante do reservatório apresentou a maior abundância (CPUE), enquanto a menor abundância foi observada na região do reservatório. Hypostomus paulinus e Hypostomus strigaticeps foram abundantes e exclusivamente amostrados na região a jusante do reservatório, enquanto Corydoras ehrhardti e Cambeva diabola apresentaram maiores abundâncias na região a montante do reservatório. A distribuição longitudinal de Siluriformes observada pode ser interpretada como um efeito negativo do represamento. Resultados obtidos indicam que os impactos negativos na ictiofauna, consequentes da construção de pequenas barragens podem ser comparáveis aos impactos originados pela construção de grandes barragens. É necessário avaliar os impactos da construção de pequenas barragens na região Neotropical, pois pouco se sabe acerca desses impactos e como estes são comparáveis a grandes reservatórios.

ABSTRACT When a river is dammed, impacts differ according to distance from the dam under consideration, and sites closer to the dam are more affected than distant ones. Damming a river changes the flow and landscape characteristics, which, in turn, change the hydrological, limnological and ecological dynamics, such as biological production, species distribution, and ecosystem structuring, functioning and services. This study evaluates the distribution pattern of the Siluriformes (catfish) fish species near a small hydroelectric power plant in the Jaguariaíva River, considering three distinct distances from the dam: upstream region, reservoir and downstream region. Samples were taken with gillnets between March 2013 and December 2014. The abundance and richness of the Siluriformes species were evaluated. A permutational multivariate analysis of variance was used to access possible differences in species composition among sites. Finally, a permutational analysis of multivariate dispersion was used to assess possible differences among sampling sites and to examine composition similarities among sites. A total of 840 Siluriformes individuals were captured, distributed among ten species and four families. The highest richness was observed in the downstream region, while the lowest richness was observed in the reservoir area. Differences in the abundance of species were observed between the three distinct regions; the downstream region presented the highest species abundance (CPUE), whereas the lowest abundance was observed in the dam region. Hypostomus paulinus and Hypostomus strigaticeps were abundant species, but exclusively sampled in the downstream region, while Corydoras ehrhardti and Cambeva diabola presented their highest abundance in the upstream region. The distinct longitudinal distribution of Siluriformes observed in this study can be interpreted as indicative of the negative effects of damming. Our study suggests that building small dams may result in negative impacts on the fish assemblage, impacts comparable to those of large reservoirs in terms of spatial changes. It is fundamentally necessary to better evaluate the environmental impacts of small dams in the Neotropical region, especially because few is known about them and how such impacts are comparable to impacts of larger reservoirs.

https://doi.org/10.1590/1678-4766e2020005 1429 downloads

11.

Genetic diversity and aquaculture conservation for a threatened Neotropical catfish
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Farias, Renata S.

; Silva, Bruno C. N. R.

; Nascimento, Wilka V. G.

; Silva, Gênison C.

; Luz, Ronald K.

; Prosdocimi, Francisco

; Figueiredo, Rozzanno A. C. R.

; Carvalho, Daniel C.

; Coimbra, Maria R. M.

Neotropical Ichthyology

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2020, Volume 18 Nº 3 elocation e200028

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Devido à importância ecológica de Lophiosilurus alexandri, o presente trabalho avaliou sua representatividade genética, comparando estoques selvagens com plantéis de reprodutores de três larviculturas ao longo do Rio São Francisco. Noventa e sete amostras foram genotipadas com marcadores microssatélites recém-desenvolvidos. Baixos níveis de diversidade genética (número médio de alelos de 4,2) foram detectados em todos os casos, sendo mais severo no cativeiro. Os valores de FST e DEST par a par, as análises do Structure e DAPC mostraram a estruturação dos animais selvagens em dois grupos, e um terceiro formado pelas larviculturas, evidenciando a necessidade de adoção de critérios genéticos para retenção da diversidade genética no cativeiro. Por essa razão, três famílias de irmãos completos foram construídas para selecionar o melhor estimador de parentesco para a espécie e estabelecer os valores mínimos de corte para evitar cruzamentos indesejados. Dois estimadores, Wang (RW) e Lynch & Li (RLL), foram eficientes em refletir as relações de parentesco para irmãos completos nessa espécie. Segundo eles, menos de 50% dos potenciais cruzamentos são recomendáveis nas três larviculturas. A baixa diversidade genética inerente ao L. alexandri destaca a importância de minimizar a consanguinidade e evitar perda de diversidade genética, visando a recuperação da espécie.

Abstract Due to the ecological importance of Lophiosilurus alexandri, the present work evaluated its genetic representativeness by comparing wild stocks to broodstocks that were kept at three restocking hatcheries along the São Francisco River. A total of 97 samples were genotyped for newly developed microsatellite markers. Low levels of genetic diversity (average alleles number of 4.2 alleles) were detected in all cases, being more severe in captive groups. Significant pairwise FST and DEST values, Structure, and DAPC analyses showed that wild animals were structured in two groups, and a third group was formed by captive animals, evidencing the need to adopt genetic criteria to retain genetic diversity in the hatcheries. For this reason, three full-sib families were constructed to select the best relatedness estimator for L. alexandri and establish a cut-off value aimed to avoid full-sibling matings in the hatcheries. Two estimators, Wang (RW) and Lynch & Li (RLL), were accurate in reflecting the relatedness level for full-sibs in this species. According to them, less than 50% of the potential breeding matings in the three hatcheries are advisable. The innate low diversity of L. alexandri highlights the importance of minimizing inbreeding and retaining genetic diversity towards the species recovery.

https://doi.org/10.1590/1982-0224-2020-0028 727 downloads

12.

Guideline of the Brazilian Society of Cardiology on Telemedicine in Cardiology - 2019
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Lopes, Marcelo Antônio Cartaxo Queiroga

; Oliveira, Gláucia Maria Moraes de

; Ribeiro, Antonio Luiz Pinho

; Pinto, Fausto J.

; Rey, Helena Cramer Veiga

; Zimerman, Leandro Ioschpe

; Rochitte, Carlos Eduardo

; Bacal, Fernando

; Polanczyk, Carisi Anne

; Halperin, Cidio

; Araújo, Edson Correia

; Mesquita, Evandro Tinoco

; Arruda, José Airton

; Rohde, Luis Eduardo Paim

; Grinberg, Max

; Moretti, Miguel

; Caramori, Paulo Ricardo Avancini

; Botelho, Roberto Vieira

; Brandão, Andréa Araújo

; Hajjar, Ludhmila Abrahão

; Santos, Alexandre Fonseca

; Colafranceschi, Alexandre Siciliano

; Etges, Ana Paula Beck da Silva

; Marino, Bárbara Campos Abreu

; Zanotto, Bruna Stella

; Nascimento, Bruno Ramos

; Medeiros, Cesar Rocha

; Santos, Daniel Vitor de Vasconcelos

; Cook, Daniela Matos Arrowsmith

; Antoniolli, Eduardo

; Souza Filho, Erito Marques de

; Fernandes, Fábio

; Gandour, Fabio

; Fernandez, Francisco

; Souza, Germano Emilio Conceição

; Weigert, Guilherme de Souza

; Castro, Iran

; Cade, Jamil Ribeiro

; Figueiredo Neto, José Albuquerque de

; Fernandes, Juliano de Lara

; Hadlich, Marcelo Souza

; Oliveira, Marco Antonio Praça

; Alkmim, Maria Beatriz

; Paixão, Maria Cristina da

; Prudente, Maurício Lopes

; Aguiar Netto, Miguel A. S.

; Marcolino, Milena Soriano

; Oliveira, Monica Amorim de

; Simonelli, Osvaldo

; Lemos Neto, Pedro A.

; Rosa, Priscila Raupp da

; Figueira, Renato Minelli

; Cury, Roberto Caldeira

; Almeida, Rodrigo Coelho

; Lima, Sandra Regina Franco

; Barberato, Silvio Henrique

; Constancio, Thiago Inocêncio

; Rezende, Wladimir Fernandes de

Arquivos Brasileiros de Cardiologia

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Nov 2019, Volume 113 Nº 5 Páginas 1006 - 1056

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190205 5274 downloads

13.

Rooting of mini-cuttings of Sequoia sempervirens using different clones and cultural environments
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de Oliveira Pereira, Mariane

; Camargo Ângelo, Alessandro

; Navroski, Marcio Carlos

; Nicoletti, Marcos Felipe

; Nascimento, Bruno

; Schatz Sá, Alexandra Cristina

; de Oliveira, Luciana Magda

; Lovatel, Queli Cristina

Bosque (Valdivia)

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2019, Volume 40 Nº 3 Páginas 335 - 346

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

SUMMARY: Sequoia sempervirens is a fast-growing, long-lived tree with pest-resistant wood. The objective of this study was to quantify the adventitious rooting ability of sequoia clones in two environments as well as determining rooting behavior during the evaluation period. Mini-cuttings of five clones grown in a clonal mini-garden were planted for rooting in two different environments: a mini-tunnel and an intermittently fogging greenhouse. Weekly assessments (from 30 to 93 days) of the variables were performed: survival, rooted mini-cuttings and number of roots emitted. Rooting speed varied in relation to the clones, occurring mainly between 50 and 60 days after planting, with the appearance of new roots between 70 and 80 days. The cultivation environment promoted distinct responses in the rooting process, being earlier in the greenhouse environment, although with a higher index in the mini-tunnel at the end of the evaluation. The process of differentiation of mini-cuttings occurs initially with the formation of calluses, around 15 days, followed by rooting (between 30 and 60 days) and the subsequent appearance of new roots. The rooting of sequoia mini-cuttings can be done in a mini-tunnel, with good indexes for all clones. It is recommended to keep mini-cuttings in this environment for 90 days for good root formation.

RESUMEN: Sequoia sempervirens es un árbol longevo de rápido crecimiento con madera resistente a plagas. El objetivo de este estudio fue cuantificar la capacidad de enraizamiento adventicio de clones de S. sempervirens en dos ambientes, así como determinar el comportamiento de enraizamiento durante el período de evaluación. Se plantaron miniestaquillas de cinco clones cultivados en minihuertos clonales para enraizar en dos ambientes diferentes: mini túnel e invernadero de nebulización intermitente. Se realizaron evaluaciones semanales (de 30 a 93 días) de las variables: supervivencia (%), mini estaquillas enraizadas (%) y raíces emitidas. La velocidad de enraizamiento varió en relación con los clones, ocurriendo principalmente entre 50 y 60 días después de plantados, con la aparición de nuevas raíces entre 70 y 80 días. El ambiente de cultivo promovió respuestas distintas en el proceso de enraizamiento, siendo más temprano en el invernadero de nebulización intermitente, pero con un índice más alto en el mini túnel al final de la evaluación. El proceso de diferenciación de mini estaquillas ocurrió inicialmente con la formación de callos, alrededor de 15 días, seguido de enraizamiento (entre 30 y 60 días) y la posterior aparición de nuevas raíces. El enraizamiento de las mini estaquillas se puede hacer en un mini túnel, con buenos índices para todos los clones, y se recomienda mantener las mini estaquillas en este ambiente durante 90 días para una buena formación de raíces.

864 downloads

14.

Variações e diferenciais da mortalidade por doença cardiovascular no Brasil e em seus estados, em 1990 e 2015: estimativas do Estudo Carga Global de Doença
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Brant, Luisa Campos Caldeira

; Nascimento, Bruno Ramos

; Passos, Valéria Maria Azeredo

; Duncan, Bruce Bartholow

; Bensenõr, Isabela Judith Martins

; Malta, Deborah Carvalho

; Souza, Maria de Fatima Marinho de

; Ishitani, Lenice Harumi

; França, Elizabeth

; Oliveira, Mateus S.

; Mooney, Meghan

; Naghavi, Mohsen

; Roth, Gregory

; Ribeiro, Antonio Luiz Pinho

Revista Brasileira de Epidemiologia

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Mai 2017, Volume 20 Supl. 1 Páginas 116 - 128

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Objetivo: Analisar as variações e os diferenciais da mortalidade por doenças cardiovasculares (DCV) no Brasil e em seus estados, em 1990 e 2015. Métodos: Foram utilizados os dados de mortalidade compilados pelo Global Burden of Disease (GBD) 2015, obtidos da base de dados do Sistema de Informação sobre Mortalidade do Ministério da Saúde. Foram realizadas a correção do sub-registro de óbitos e a reclassificação dos códigos garbage por meio de algoritmos específicos. As causas cardiovasculares foram subdivididas em 10 causas específicas. As taxas de mortalidade - dos anos 1990 e 2015 - foram padronizadas pela idade, de acordo com o sexo e o estado brasileiro. Resultados: A taxa de mortalidade por DCV padronizada por idade caiu de 429,5 (1990) para 256,0 (2015) a cada 100 mil habitantes (40,4%). A redução proporcional foi semelhante em ambos os sexos, mas as taxas em homens são substancialmente mais altas do que nas mulheres. A redução da taxa padronizada por idade foi mais acentuada para a doença cardíaca reumática (44,5%), cardiopatia isquêmica (43,9%) e doença cerebrovascular (46,0%). A queda na mortalidade diferiu marcadamente entre os estados, sendo mais acentuada nos estados das regiões Sudeste e Sul do país e no Distrito Federal, e atenuada nos estados do Norte e Nordeste. Conclusão: A mortalidade por DCV padronizada por idade reduziu no Brasil nas últimas décadas, porém de forma heterogênea entre os estados e para diferentes causas específicas. Considerando a magnitude da carga de doença e o envelhecimento da população brasileira, as políticas de enfrentamento das DCV devem ser priorizadas.

ABSTRACT: Objective: To analyze variations and particularities in mortality due to cardiovascular disease (CVD) in Brazil and in Brazilian states, in 1990 and 2015. Methods: We used data compiled from the Global Burden of Disease (GBD) 2015, obtained from the database of the Mortality Information System (SIM) of the Brazilian Ministry of Health. Correction of the sub-registry of deaths and reclassification of the garbage codes were performed using specific algorithms. The cardiovascular causes were subdivided into 10 specific causes. Age-standardized CVD mortality rates - in 1990 and 2015 - were analyzed according to sex and Brazilian state. Results: Age-standardized CVD mortality rate decreased from 429.5 (1990) to 256.0 (2015) per 100,000 inhabitants (40.4%). The proportional decrease was similar in both sexes, but death rates in males were substantially higher. The reduction of age-standardized mortality rate was more significant for rheumatic heart disease (44.5%), ischemic cardiopathy (43.9%), and cerebrovascular disease (46.0%). The decline in mortality was markedly different across states, being more pronounced in those of the southeastern and southern regions and the Federal District, and more modest in most states in the north and northeast regions. Conclusion: Age-standardized CVD mortality has declined in Brazil in recent decades, but in a heterogeneous way across states and for different specific causes. Considering the burden magnitude and the Brazilian population aging, policies to prevent and manage CVD should continue to be prioritized.

https://doi.org/10.1590/1980-5497201700050010 1557 downloads

15.

Variações e diferenciais da mortalidade por doença cardiovascular no Brasil e em seus estados, em 1990 e 2015: estimativas do Estudo Carga Global de Doença
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Brant, Luisa Campos Caldeira

; Nascimento, Bruno Ramos

; Passos, Valéria Maria Azeredo

; Duncan, Bruce Bartholow

; Bensenõr, Isabela Judith Martins

; Malta, Deborah Carvalho

; Souza, Maria de Fatima Marinho de

; Ishitani, Lenice Harumi

; França, Elizabeth

; Oliveira, Mateus S.

; Mooney, Meghan

; Naghavi, Mohsen

; Roth, Gregory

; Ribeiro, Antonio Luiz Pinho

Revista Brasileira de Epidemiologia

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Mai 2017, Volume 20 Supl. 1 Páginas 116 - 128

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1980-5497201700050010 9207 downloads

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