ABSTRACT The use of auxin mimics herbicides in agriculture is widespread in a great diversity of crops. The indiscriminate use of herbicides can cause adverse effects in the plants. Among the plant species that stand out for being highly competitive and resistant to biotic and abiotic stresses, it is the brazilian peppertree (Schinus terebinthifolius Raddi.). The objective of this work was to evaluate the phytotoxic and photosynthetic alteration effects of different rates simulating the drift of the herbicides 2,4-D and Dicamba in brazilian peppertree seedlings. The experiment was carried out in a completely randomized design with four replications. The treatments were arranged in 2 x 8 factorial design (herbicide x doses). The factor herbicide consisted the herbicides 2,4-D and Dicamba, and, the factor doses eight percentages of the herbicide applied. Phytotoxicity and alteration of photosynthetic and fluorescence of chlorophyll a parameters were evaluated. Increased rate of the 2,4-D and Dicamba cause phytotoxicity to the plants, whose Dicamba promotes greater injuries. Dicamba was the herbicide that caused the greatest damage to the photosynthetic apparatus on brazilian peppertree plants, while for 2,4-D the plants showed higher recovery potential after herbicidal exposure.

RESUMO: O estudo quantificou os parâmetros cinéticos de captação de K nas gramíneas Paspalum notatum, Paspalum urvillei e Axonopus affinis associados com o crescimento. Dez mililitros (10 ml) de solução nutritiva foram coletados em vasos de dois litros - com cinco repetições em 24 horas - para determinação das concentrações de K nas amostras. A matéria seca da parte aérea e raízes; comprimento, volume, diâmetro e área superficial radicular, bem como parâmetros cinéticos associados à absorção de nutrientes (Vmax, Km, Cmin e I) foram avaliados. A espécie P. notatum apresentou a maior produção de raiz, parte aérea e MS total, bem como o maior volume de raiz. P. urvillei apresentou o maior teor de K, que pode ser associado a raízes mais finas e resultando em maior área superficial. A. affinis registrou o maior valor de Vmax, o que levou a uma maior eficiência de absorção de K.

ABSTRACT: This study quantified K uptake kinetic parameters in grass species Paspalum notatum, Paspalum urvillei and Axonopus affinis to associate them with growth. Ten milliliters (10 ml) of nutrient solution were collected in two-liter pots - with five repetitions in 24 hours - in order to determine K concentrations in the samples. Shoot and root dry matter, root length, volume, diameter and surface area, as well as kinetic parameters associated with nutrient uptake (Vmax, Km, Cmin and I) were determined. Species P. notatum was the one presenting the highest root, shoot and total dry matter production, as well as the highest root volume. P. urvillei recorded the highest K content, which may be associated with thinner roots and greater root surface area. A. affinis recorded the highest Vmax value, which led to greater K uptake efficiency.

Resumo O desenvolvimento de plantas mais eficientes no uso de nutrientes será fundamental para a agricultura nos próximos anos. O objetivo deste trabalho foi definir um marcador fisiológico do status nutricional do P para clones de batata cultivados fora do solo e in vitro, visando facilitar o processo de seleção de clones mais eficientes no uso de P. Para tanto, foram realizados dois experimentos com sete clones de batata (SMIC 148-A, Dakota Rose, SMINIA 793101-3, SMIB 106-7, SMIF 212-3, SMIJ 319-1 e P 150) cultivados em dois níveis de P (baixo e alto) no sistema de cultivo in vitro (1,935 e 19,346mg P L-1) e no cultivo fora do solo (2,32 e 23,2mg P L-1) usando areia como substrato. O teor de P inorgânico e a acumulação total de P na parte aérea e nas raízes parecem ser bons indicadores da condição nutricional de P em plantas de batata, tanto no cultivo fora do solo quanto no in vitro. A atividade das enzimas fosfatases ácidas (APases) nos tecidos da batata depende muito do sistema de cultivo, do clone e do órgão da planta analisado, dificultando a escolha deste como um marcador fisiológico para restrição de P em batata. A massa seca da planta de batata pode ser um bom indicador do status nutricional do P em clones cultivados fora do solo.

Abstract The development of more efficient plants in the use of nutrients will be fundamental for agriculture in the coming years. The objective of this work was to define a physiological marker of the nutritional status of P for potato clones cultivated soilless and in vitro, in order to facilitate the selection process of more efficient clones in the use of P. For this, two experiments were carried out with seven potato clones (SMIC 148-A, Dakota Rose, SMINIA 793101-3, SMIB 106-7, SMIF 212-3, SMIJ 319-1 and P 150) grown at two levels of P (low and high) in the culture systems in vitro (1.935 and 19.346mg P L-1) and in soilless (2.32 and 23.2mg P L-1) using sand as substrate. The inorganic P content and the total accumulation of P in shoot and roots seem to be good indicators of the nutritional status of P in potato plants, both in soilless and in vitro cultivation. The activity of the acidic phosphatase enzymes (APases) in the tissues of the potato depends very much on the culture system, the clone and the organ of the analyzed plant, making it difficult to choose it as a physiological marker for restriction of P in potatoes. The dry mass of the potato plant may be a good indicator of the nutritional status of P in clones grown soilless.

ABSTRACT South American natural grasslands are composed of several species with different growth strategies, with variations in specific leaf area (SLA), leaf dry matter content (LDMC), specific root length (SRL) and specific root area (SRA). The objective of this study was characterizing in grasses cultivated with different levels of phosphorus (P) in the soil if species with higher leaf and root area production per unit of dry matter have higher tissue P concentration, P use efficiency and higher dry matter yield. The plant species were grown in a greenhouse in pots with 5 kg of soil in a completely randomized design with four replicates and two conditions of P availability: addition of 60 mg kg-1 soil and without addition of P. The species with the highest SRA had a higher leaf and root P concentration. The higher production of leaf or root surface area per unit of dry matter did not represent higher tissue P use efficiency. The group formed by species of genre Paspalum had a higher leaf and root P use efficiency, therefore, areas composed of this genre are preferred for P fertilization.

RESUMO: O objetivo deste trabalho foi avaliar a interação entre herbicidas associados ao saflufenacil para o controle de capim-arroz e a determinação do efeito dos herbicidas sobre os parâmetros fotossintéticos e de fluorescência de clorofila. O experimento foi conduzido em casa de vegetação em esquema fatorial 2x8, cujo fator A testou os biótipos resistente e suscetível; e o fator B os herbicidas: saflufenacil (70 g i.a. ha-1), clomazone (180 g i.a. ha-1), imazapyr+imazapic (73,5+24,5 g i.a. ha-1), cyhalofop (360 g i.a. ha-1), as associações desses com saflufenacil, e testemunha sem tratamento. Foi avaliado o controle aos 7, 14, 21 e 28 dias após a aplicação dos herbicidas (DAA), massa seca da parte aérea aos 28 DAA, avaliação de parâmetros fotossintéticos com analisador de gás infravermelho (IRGA) e emissão de fluorescência da clorofila a 24 e 48 horas após aplicação dos tratamentos, respectivamente, e interação dos herbicidas. A associação de saflufenacil com herbicidas testados na maior parte não modificou a resposta dos herbicidas para o controle de capim-arroz em ambos os biótipos. O biótipo resistente apresentou menor efeito negativo nos processos de fluorescência de clorofila e parâmetros de fotossíntese na associação de herbicidas com saflufenacil. O herbicida cyhalofop associado ao saflufenacil demonstra ser eficiente para o controle de capim-arroz suscetível e resistente a ALS.

ABSTRACT: The objectives of this research were to evaluate the interaction between herbicides mixed with saflufenacil for the control of barnyardgrass and to determine the effect on photosynthetic and chlorophyll fluorescence parameters. The experiment was conducted in a greenhouse in a 2x8 factorial scheme, whose factor A tested resistant and susceptible biotypes; and factor B the herbicides: saflufenacil (70 g a.i. ha-1), clomazone (180 g a.i. ha-1), imazapyr + imazapic (73.5 + 24.5 g a.i. ha-1), and cyhalofop (360 g a.i. ha-1), the mixtures of these herbicides with saflufenacil, and control without treatment. Weed control was assessed 7, 14, 21 and 28 days after herbicide application (DAA), as well as shoot dry matter at 28 DAA, photosynthetic parameters using infrared gas analyzer (IRGA), and emission of chlorophyll a fluorescence after 24 and 28 hours of application of treatments, respectively, and interaction of herbicides. Combination of saflufenacil with the herbicides tested in general did not change the response of both barnyardgrass biotypes to the herbicides used. The resistant biotype showed a lower negative effect on chlorophyll fluorescence and photosynthesis parameters in the combination of herbicides with saflufenacil. The herbicide cyhalofop was effective for the control of ALS-susceptible and resistant barnyardgrass.

Abstract Abstract: Successive applications of copper fungicides on vines have resulted in increased copper content in vineyard soils over the years. This high copper content has affected the growth of young vines in eradicated vineyards. Thus, the cultivation of annual species for a few years is an alternative to copper phytostabilization, because it would be a good way to decrease copper availability to plants. The aim of this study was to evaluate the physiological responses of different soybean cultivars to copper concentration increase. Four different soybean cultivars were grown under three copper concentrations: 0.5, 20 and 40 μM in nutrient solution. The main outcomes of this study were: i) Cultivar M 6410 IPRO recorded the highest photosynthetic rate when plants were exposed to 40 μM of copper in the nutrient solution; ii) plants in cultivar M 6410 IPRO accumulated large copper concentrations in their roots although did not decrease the root dry mass, possibly due to the higher superoxide dismutase activity; iii) cultivar DM 5958 RSF IPRO recorded drastically reduced photosynthetic rate and dry mass production due to copper excess. We conclude that each cultivar responded differently to the excess of copper, but none of them showed tolerance to it.

RESUMO: O carbono rizosférico resultado da exsudação radicular é um dos substratos utilizados pela microbiota do solo, refletindo-se nas emissões de metano (CH4) em ambientes anóxicos, como no cultivo de arroz irrigado. Com o aumento da capacidade fotossintética da planta no período reprodutivo, ocorre acúmulo maior de biomassa, que por sua vez, impulsiona aumento na taxa de exsudação radicular. Entretanto, variações genotípicas nos aspectos fisiológicos das plantas de arroz podem se relacionar com a quantidade de exsudatos radiculares. Foram avaliadas dez cultivares de arroz irrigado quanto a taxa de exsudação de carbono orgânico total (TEXCOT), matéria seca da parte aérea (MSPA) e variáveis fisiológicas relacionadas com a fotossíntese no período de florescimento pleno. Dois experimentos foram conduzidos na casa de vegetação do Departamento de Solos da UFSM em delineamento experimental inteiramente casualizado. As cultivares apresentaram diferenças significativas na TEXCOT, na MSPA e em todas as variáveis fisiológicas, bem como, correlações positivas e significantes entre TEXCOT e as variáveis fisiológicas, sendo que as cultivares de ciclo precoce se mostraram mais ineficientes nas variáveis fisiológicas refletindo em valores reduzidos de TEXCOT e MSPA, enquanto que as cultivares de ciclo médio se mostraram mais eficientes com reflexos em maiores TEXCOT e MSPA.

ABSTRACT: Rhizospheric carbon resulting from root exudation is one of the substrates used by the soil microbiota, and reflects methane (CH4) emissions in anoxic environments such as irrigated rice cultivation. With the increase of the photosynthetic capacity of the plant in the reproductive period, there is greater accumulation of biomass which, in turn, increases the rate of root exudation. However, genotypic variations in the physiological aspects of rice plants may be related to the amount of root exudates. Ten cultivars of irrigated rice were evaluated for the exudation rate of total organic carbon (EXRToc), shoot dry matter (SDM), and physiological variables related to photosynthesis during the full flowering (blooming) period. Two experiments were conducted in the greenhouse of the Department of Soils of the UFSM (University of Santa Maria, Santa Maria, RS, Brazil) in a completely randomized experimental design. The cultivars presented significant differences in EXRToc, SDM, and all physiological variables as well as positive and significant correlations between EXRToc and physiological variables. Early cultivars were more inefficient in the physiological variables reflecting reduced values of EXRToc and SDM whereas medium-cycle cultivars were more efficient with larger EXRToc and SDM reflections.

ABSTRACT Crotalaria juncea is used as plant cover in grape vineyards in Brazil, which usually present soils with high copper (Cu) levels due to the application of Cu-based phyto-sanitary products. Under this condition an increase growth and cover of C. juncea is needed to improve the phytoremediation processes in those soils. Some alternatives to achieve this condition is the inoculation with arbuscular mycorrhizal fungi (AMF), which has demonstrated an important increase of plant growth in Cu-contaminated soils at different soil P levels. The aims of this study were to evaluate the effect of AMF inoculation in soils with high Cu contents on the growth of C. juncea, the acid phosphatase (APase) enzyme activity in plants and soil, and the presence of glomalin under different P supply conditions, as a basis to identify if there is a synergistic interaction between AMF inoculation and P supply on soils with high Cu levels. The experiment was carried under greenhouse conditions in a factorial 3 × 2 design (natural P content, addition of 40 and 100 mg kg-1 P, with and without the inoculation of the AMF Rhizophagus clarus with three replicates) in a soil with high Cu content (60 mg kg-1). The addition of 40 and 100 mg kg-1 P favored plant growth both in the presence and in the absence of AMF. However, when plants were grown in soil with a natural P level, the inoculation with AMF increased by 116 % the shoot biomass, compared to the non-inoculated treatment. Our results showed that the combination of P supply and R. clarus inoculation could be an adequate strategy to reduce Cu phytotoxicity in C. juncea, as it increases plant biomass and modify the APase enzyme activity in the soil and plant. Additionally, glomalin produced by the AMF and accumulated in the soil can decrease the availability of Cu to the plants by means of sequestration beyond the root surface, with a consequent plant protective effect.

ABSTRACT A hydroponic experiment was carried out to investigate the effect of phosphorus (P) nutrition on arsenic (As) uptake and translocation within the seedlings of rice cultivars. The experiment occurred in three stages: I 5 days of acclimatization (nutritive solution); II 10 days under P (0.0 and 0.09 mM) and As (0.0 and 100 mM) treatments; III 5 days under recovery. The As exposure had significant effect reducing dry weights of shoots or roots, resulted in elevated concentrations of As in shoot tissues. BR-IRGA 409 showed the highest susceptibility to As in biomass production and root system parameters regardless the P level. This cultivar showed contrasting responses of As translocation to shoot tissue dependent on P levels, with the highest As concentration under low P and lowest under normal P levels. P nutrition was most striking on plants recovery for all cultivars under As exposure. Clearer separation of cultivars for phosphorus use efficiency (PUE) occurred at lower shoot P contents, that was, at higher levels of P deficiency stress. IRGA 424 showed higher PUE as compared to the others cultivars. Our results go some way to understanding the role of P nutrition in controlling the effects of As in rice shoots.

RESUMO: A presença de uma camada compactada próxima à superfície do solo em áreas de várzeas pode limitar o crescimento e desenvolvimento das raízes de soja. O objetivo do trabalho foi avaliar diferentes manejos de implantação e irrigação no crescimento e desenvolvimento do sistema radicular de soja em área de várzea. O experimento foi realizado nas safras 2013/14 e 2014/15 no delineamento experimental de blocos ao acaso em esquema fatorial (3x2), em faixa, com quatro repetições. Os tratamentos constaram de diferentes manejos de implantação da cultura: semeadura com disco duplo desencontrado (A1); semeadura com haste sulcadora (A2) e escarificação do solo + semeadura com disco duplo desencontrado (A3), e de irrigação: com irrigação (D1) e sem irrigação (D2). Os manejos de implantação e a irrigação influenciaram no crescimento das raízes de soja. No disco duplo, as raízes apresentam menor crescimento e aumento do diâmetro. Os manejos com haste sulcadora e escarificação do solo proporcionam maior crescimento e desenvolvimento de raízes de soja e maior distribuição em profundidade. Uma irrigação suplementar de 55mm no estádio V4 de desenvolvimento das plantas de soja proporciona aumento da área superficial e do volume de raízes em soja, quando a umidade do solo atinge valores abaixo de 60% da capacidade de campo.

ABSTRACT: The presence of a compacted soil layer near the ground surface in paddy fields may limit the growth and development of soybean roots. The objective of this study was to evaluate different planting management systems and irrigation on growth and development of soybean root systems in lowland area. The experiment was carried out in 2013/14 and 2014/15 crop seasons in randomized complete block design with factorial treatment (3x2), with four replications. The treatments consisted of different planting management systems: sowing with double disc (A1); sowing with shank (A2) and deep tillage + sowing with double disc (A3), and irrigation: irrigated (D1) and non irrigated (D2). Planting management systems and irrigation influenced the growth of soybean roots. When double disc was used, roots have lower growth and increase in diameter. Use of shanks and deep tillage provide increased growth and development of soybean roots and greater depth distribution. An additional 55mm of irrigation during the V4 soybean development stage provides increased surface area and root volume in when the soil moisture reaches values below 60% of field capacity.

RESUMO: O alumínio (Al) é altamente tóxico para as plantas, causando estresse e inibindo o crescimento e o silício (Si) é considerado benéfico para as plantas. Este elemento químico tem uma alta afinidade com o Al. O objetivo deste estudo foi investigar o potencial do Si em amenizar os efeitos tóxicos do Al sobre plantas de batata ( Solanum tuberosum L.) e avaliar se esse comportamento é diferente entre os genótipos com diferente sensibilidade ao Al. Plantas de batata dos genótipos SMIJ319-7 (sensível ao Al) e SMIF212-3 (tolerante ao Al) foram cultivadas por 14 dias em solução nutritiva (sem P e pH 4,5±0,1), sob exposição a combinações de Al (0 e 1,85mM) e Si (0; 0,5 e 1,0mM). Após esse período, parte aérea e raízes dos dois genótipos foram coletadas para determinar o conteúdo de Al nos tecidos e avaliar parâmetros morfológicos das raízes e parte aérea. Raízes de ambos os genótipos acumularam mais Al do que a parte aérea, e o genótipo tolerante ao Al acumulou mais Al do que o sensível, tanto nas raízes quanto na parte aérea. Além disso, a presença de 0,5 e 1,0mM de Si juntamente com Al reduziu o conteúdo de Al na parte aérea em ambos os genótipos e nas raízes do genótipo tolerante ao Al, respectivamente. O Si amenizou os efeitos tóxicos do Al para número de ramificações de raízes e de folhas em ambos os genótipos de batata. Si tem o potencial para amenizar os efeitos tóxicos do Al em plantas de batata, independente da sensibilidade ao Al.

ABSTRACT: Aluminum (Al) is highly toxic to plants, causing stress and inhibiting growth and silicon (Si) is considered beneficial for plants. This chemical element has a high affinity with Al. The aim of this study was to investigate the potential of Si to mitigate the toxic effects of Al on potato ( Solanum tuberosum L.) plants and assess whether this behavior is different among genotypes with differing degrees of sensitivity to Al. Potato plants of the genotypes SMIJ319-7 (Al-sensitive) and SMIF212-3 (Al-tolerant) were grown for fourteen days in nutrient solution (without P and pH 4.5±0.1) under exposure to combinations of Al (0 and 1.85mM) and Si (0, 0.5 and 1.0mM). After this period, shoot and roots of the two genotypes were collected to determine Al content in tissues and assess morphological parameters of root and shoot growth. Roots of both genotypes accumulated more Al than shoots and the Al-tolerant genotype accumulated more Al than the sensitive one, both in roots and in shoot. Furthermore, the presence of 0.5 and 1.0mM Si together with Al reduced the Al content in shoot in both genotypes and in roots of the Al-tolerant genotype, respectively. Si ameliorated the toxic effects of Al with regard to number of root branches and leaf number in both potato genotypes. Si has the potential to mitigate the toxic effects of Al in potato plants regardless of Al sensitivity.

RESUMO: A homeostase nutricional mineral em resposta a diferentes concentrações de Hg (0, 25 e 50μM) foram avaliadas em plantas de Pfaffia glomerata. A exposição ao mais alto nível de Hg (50µM) causou um decréscimo de 15,5% e 20%, respectivamente, na matéria fresca da parte aérea e raízes. As concentrações de Hg na parte aérea e raízes aumentaram linearmente com o aumento das concentrações de Hg. A concentração de Ca decresceu na parte aérea somente em 50µM Hg, enquanto as concentrações de K e Mg na parte aérea decresceram tanto em 25 como em 50µM Hg, quando comparado ao controle. Observou-se um significativo decréscimo nas concentrações de Cu, Zn, Fe e Mn nas plantas expostas ao Hg, mas principalmente Zn, Mn e Cu nas raízes. Por outro lado, a concentração de P aumentou em raízes e parte aérea de plantas expostas a 25 e 50µM Hg, enquanto a concentração de Na aumentou somente nas raízes em 25 e 50µM Hg. No geral, as concentrações de nutrientes nos tecidos de P. glomerata expostas ao Hg foram significativamente diminuídas, o que indica que o Hg pode causar alterações na homeostase nutricional mineral dessa espécie.

ABSTRACT: The mineral nutritional homeostasis in response to different concentrations of Hg (0, 25 and 50μM) was evaluated in Pfaffia glomerata plant. The exposure to the highest level of Hg (50µM) caused a decreasing in shoot and root fresh weights of 15.5% and 20%, respectively. Both shoot and root Hg concentrations increased linearly with increasing external Hg concentrations. Ca concentration decreased in shoot only at 50µM Hg, whereas shoot K and Mg concentrations decreased at both 25 and 50µM Hg, when compared to the control. A significant decrease in Cu, Zn, Fe and Mn concentrations in plants exposed to Hg was observed, but most Zn, Mn, and Cu in the roots. On the other hand, P concentration increased in both root and shoot of plants exposed at 25 and 50µM Hg, whereas Na concentration increased only in the root at 25 and 50µM Hg exposure. In general, tissue nutrient concentrations in P. glomerata plantlets exposed to Hg were significantly decreased, which indicates that the Hg may cause alteration on the mineral nutritional homeostasis of this species.

A toxidez do alumínio (Al) é um fator limitante da produção agrícola em solos ácidos, os quais cobrem cerca de 60% do território Brasileiro. O objetivo deste estudo foi avaliar os efeitos do Al no crescimento e na concentração de Al nos tecidos de dois acessos de Pfaffia glomerata (BRA e JB/UFSM). As plantas foram cultivadas em sistema hidropônico, contendo cinco concentrações de Al (0, 50, 100, 150 e 200mg L-1) por 7 dias. Para a maioria dos parâmetros avaliados, houve interação significativa entre os dois acessos de P. glomerata e as concentrações de Al, sendo que, de modo geral, os tratamentos com Al afetaram negativamente o crescimento das plantas, especialmente as raízes. Além disso, o acesso BRA acumulou mais Al nos tecidos que o acesso JB/UFSM e, consequentemente, os parâmetros de crescimento foram afetados mais significativamente naquele acesso. Portanto, nossos resultados sugerem que, entre os acessos de P. glomerata estudados, BRA é menos indicado para usos medicinais quando cultivado em solos com altos níveis de Al, por acumular mais Al em seus tecidos.

Aluminum (Al) toxicity is a limiting factor for crop production in acid soils, which cover approximately 60% of the Brazilian territory. This study aimed to evaluate the effects of Al on growth and tissue Al concentration of two Pfaffia glomerata accessions (BRA and JB/UFSM). Plantlets were grown in a hydroponic system with five Al concentrations (0, 50, 100, 150 and 200mg L-1) for 7 days. Most of the evaluated parameters presented significant interaction between both P. glomerata accessions and Al levels in nutrient solution and, in general, Al treatments negatively affected plant growth, especially roots. Moreover, BRA accession showed higher Al accumulation in its tissues than JB/UFSM and, consequently in BRA accession the growth was impaired substantially. Furthermore, the results suggest that, between P. glomerata accessions studied, BRA is less appropriated for medicinal uses when grown in soils with high Al levels, due to the higher accumulation of tissue Al content.

RESUMO A adoção de práticas de manejo que garantam a estabilidade de matéria orgânica do solo (MOS) também sustenta a estabilidade ou o incremento quantitativo do carbono (C) na litosfera, diminuindo a quantidade do CO2 na atmosfera. Também é possível minimizar as perdas de C para a atmosfera a partir da utilização de práticas conservacionistas, ou utilização de coberturas vegetais que mantenham os estoques de C do solo, sendo que as coberturas florestais são consideradas grandes sequestradoras e os sistemas florestais considerados grandes reservatórios de C. Este trabalho foi realizado em uma propriedade rural situada no interior do município de Chapecó-SC, onde foram amostrados solos de diferentes formações florestais distribuídas em uma faixa homogênea do solo. O clima do local é mesotérmico, chuvoso, e o solo foi caracterizado como uma associação Cambissolo Háplico/Neossolo Litólico. Os objetivos deste trabalho foram estimar os estoques de C nos solos e as perdas de C ocorridas em função da alteração da cobertura do solo. Foram avaliados solos sob floresta natural (FN), de formação secundária, com elevado grau de preservação; plantio de eucalipto (Eucalyptus saligna) (PE), com oito anos de cultivo, precedido de 17 anos de lavoura sob plantio convencional; plantio de erva-mate (Ilex paraguariensis) (EM), com 25 anos de cultivo sob sistema convencional (corte em intervalo de 18 meses, com retirada de todos resíduos produzidos e manutenção do solo sem cobertura, com utilização periódica de herbicida - glifosato). Em cada área foram abertas quatro trincheiras de 50 cm de profundidade, onde foram realizadas as coletas de solo nas profundidades de: 0-5 cm; 5-10 cm; 10-20 cm; 20-30 cm; 30-40 cm, e 40-50 cm, com anéis de kopeck. Foi possível determinar a densidade do solo (Mg m-3) e o volume do solo por camada (profundidade) e por hectare, bem como a concentração de C do solo nas diferentes áreas estudadas. Para quantificar os estoques de C foram utilizadas quantidades iguais de solo para cada profundidade avaliada. Foram observadas maiores densidades dos solos sob PE e EM, sendo que em FN os menores valores de densidade são explicados pelo grande aporte de material orgânico e ausência de efeito antrópico. Em FN, apesar da menor densidade do solo, verificou-se maior estoque de C no solo, com 107,67 Mg C ha-1. No solo sob PE, com estoque de 79,58 M g C ha-1, em função da utilização anterior (17 anos com lavoura sob cultivo convencional), supõe-se que parte do C tenha sido recuperada. Sob EM, com estoque de 47,29 Mg C ha-1, as perdas de C foram evidentes, com cerca de 221 Mg CO2ha-1 emitidas do solo. Ficou evidente que a alteração da cobertura florestal e o manejo utilizado podem levar a grandes perdas do C estocado. Assim, o solo sob florestas, ou o sistema solo-planta de coberturas florestais, considerado um grande reservatório de C, pode tornar-se uma grande fonte de C para a atmosfera, contribuindo para aumentar o efeito estufa.

ABSTRACT The adoption of management practices that ensure the stability of soil organic matter also maintain the stability or quantitative increase of carbon (C) in the lithosphere, reducing the amount of CO2 in the atmosphere. You can also minimize the losses of C to the atmosphere by using conservation practices, or using cover crops to keep the soil C stocks, and the forest cover are considered great abduction and forest systems considered large reservoirs of C. This work was performed on a property located in Chapecó, Santa Catarina state, where soils were sampled from different forest formations distributed in a homogeneous soil range. The local climate is mesothermal, rainy, and the soil was characterized as an association Cambissolo Háplico/Neossolo Litólico. The objectives were to estimate the C stocks in soils and estimate the C losses occurred due to the change of soil cover. It was evaluated soils under natural forest (FN), of secondary stage, with a high degree of preservation; planted forest of eucalyptus (Eucalyptus saligna) (PE), with eight years of cultivation, preceded by 17 years under crop conventional tillage; and a planted forest of herb mate (Ilex paraguariensis) (EM), with 25 years of cultivation under conventional system (cutting interval of 18 months, with removal of all waste produced and maintenance of the ground without cover, with periodic use of herbicide - glyphosate). In each area were opened four trenches with 50 cm deep, where soil samples were collected in depths of: 0-5 cm, 5-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, and 40-50 cm, with kopeck rings. It was possible to determine the bulk density (Mg m-3), the soil volume per layer (depth) and per hectare, and the concentration of soil C in the different studied areas. To quantify the C stocks equal amounts of soil were used for each depth evaluated. It was observed higher densities of soils and under PE and EM, to FN the lowest density are explained by the large amount of organic material and the absence of anthropogenic effects. In FN, despite the lower bulk density, there was a greater soil content C, with 107.67 Mg C ha-1. On the soil under PE, with stock 79.58 Mg C ha-1, depending on previous use (17 years under crop conventional tillage), it is assumed that part of C has been recovered. Under EM, with stock of 47.29 Mg C ha-1, C losses were evident, with about 221 Mg CO2 ha-1 emitted from the soil. It was evident that the change in forest cover and management procedures can lead to large losses of C stored. Thus, the soil under forest, or the soil-plant a forest canopy, considered a major reservoir of C, can become a major source of C to the atmosphere, contributing to increased the greenhouse effect.

Apesar da grande disponibilidade de informações sobre as etapas in vitro da micropropagação de batata, ainda não se conhece os seus efeitos sobre o comportamento das plantas durante a aclimatização. Desse modo, este trabalho teve como objetivo verificar a influência da concentração dos sais e da sacarose do meio MS, tanto na multiplicação in vitro quanto na aclimatização das mudas de batata em sistema de cultivo sem solo. O experimento foi conduzido em laboratório e telado, em um fatorial no delineamento inteiramente ao acaso, com quatro repetições de 20 explantes. Em laboratório foi avaliado o crescimento das plantas de três clones (Asterix, Macaca e SMINIA793101-3) de batata em função da combinação de concentrações dos sais (½ MS e 1 MS) e de sacarose (30, 45 e 60 g L-1) do meio MS. Em telado foi avaliada a aclimatização das mudas destes tratamentos em dois sistemas (placa flutuante e substrato mineral) de cultivo sem solo. Durante o cultivo in vitro, exceto para o número de raízes, os clones avaliados mostraram resposta morfogênica variável. A elevação da concentração de sais do meio MS aumentou o número de folhas de 4,4 para 4,7 e diminuiu o número de raízes de 3,7 para 3,4. O incremento da concentração de sacarose do meio MS reduziu o número de folhas de 5,0 para 4,1 e o comprimento da parte aérea de 2,4 para 2,1 cm. Apenas as concentrações de sais do meio MS influenciaram a sobrevivência ex vitro das mudas de batata, sendo que a redução da concentração promoveu o aumento da sobrevivência, passando de 94,8% para 97,5%. A aclimatização em sistema de cultivo sem solo não é limitante à produção de mudas de batata, podendo ser realizada tanto em placa flutuante quanto em substrato mineral, subirrigados com solução nutritiva.

Despite the good technological understanding about in vitro micropropagation of potato, there is no information regarding plantlet behavior during acclimatization. Thus, the aim of this work was to evaluate the effect of MS salt and sucrose concentrations in the in vitro multiplication and acclimatization of potato plantlets in soilless system. The experiment was carried out in laboratory and greenhouse as a factorial in a complete random design, with four replications of 20 explants. The in vitro multiplication of three potato clones (Asterix, Macaca, and SMINIA793101-3) was assessed in different salt (half and complete medium) and sucrose (30, 45 and 60 g L-1) concentrations of the MS medium. Plantlets of each treatment were acclimatized in two soilless systems (floating and mineral substrate) under greenhouse conditions. During in vitro multiplication, there were differences among clones, except for root number. Increasing the salt concentration of the MS medium increased the number of leaves from 4.4 to 4.7, and reduced the number of roots from 3.7 to 3.4. Increasing the sucrose concentration of the MS medium reduced the number of leaves from 5.0 to 4.1 and the shoot length from 2.4 to 2.1 cm. Only the MS salt concentrations affected the ex vitro survival of potato plantlets; reducing salt concentration increased the survival from 94.8% to 97.5%. Acclimatization in soilless system is not a limiting step for the production of potato plantlets either in floating or mineral substrate, underirrigated with nutrient solution.