Abstract To assess the in vitro and in situ effect of experimental combined fluoride and calcium nanocomposite solutions on dental caries prevention. Nanocompound mesoporous silica (MS) with calcium (Ca) and sodium fluoride (NaF) - (MSCaNaF); MS with NaF (MSNaF), NaF solution (positive control), and deionized water (negative control - CG) were studied. The specimens (n=130) were submitted in vitro to a multispecies biofilm in the presence of 2% sucrose. After 24 h and 48 h, the culture medium pH, the percent of surface mineral loss (%SML), and lesion depth (ΔZ) were analyzed. In the in situ study, 10 volunteers participated in four phases of 7-days each. The products were applied on the specimens (n=240) before 20% sucrose solution drips. The polysaccharides (SEPS and IEPS), %SML and roughness (Sa) were evaluated. There was an in vitro decrease in pH values in 24h and 48h, compared to baseline. The MSCaNaF and MSNaF groups obtained lower values of %SML and ΔZ (p < 0.05) than CG and NaF after 24h and were similar to NaF after 48h (p<0.05). In situ results showed similar SEPS and IEPS among all groups after 48h. An after 7-days, the nanocomposites had similar values (p>0.05), while NaF was similar to CG (p>0.05). After 48h, the MSCaNaF and MSNaF reduced the %SML (p<0.05). After 7-days, both experimental nanocomposites were similar to NaF (p>0.05). Regarding Sa, MSCaNaF was better than NaF for both periods (p<0.05). The nanocomposites controlled the in vitro and in situ enamel demineralization, mainly in the initial periods.

ABSTRACT Background: Recent studies show an increase in nonalcoholic fatty liver disease (NAFLD) in populations with higher consumption of red meat, processed and cooked at high temperatures. On the other hand, the single nucleotide polymorphism rs738409 in the Patatin-like phospholipase domain containing 3 (PNPLA3) gene has been implicated in susceptibility to NAFLD and liver fibrosis. However, the synergistic effect between red meat consumption and the PNPLA3 gene polymorphism in NAFLD has not yet been evaluated. Objective: To evaluate the association between the presence of the polymorphism in the PNPLA3 gene and the consumption of macronutrients, including meat consumption and its cooking method among NAFLD patients. Methods: This was a cross-sectional study with 91 patients diagnosed with NAFLD by liver biopsy with genotyping for the polymorphism in the PNPLA3 gene were included. The consumption of calories and macronutrients was verified using the semi-quantitative food frequency questionnaire and the specific questionnaire on meat consumption. PNPLA3 gene polymorphism was analyzed by real-time polymerase chain reaction (RT-PCR) and anthropometric evaluation was realized. Results: The mean BMI was 32.38±4.58 kg/m² and the waist circumference was 107±10 cm. On liver biopsy, 42% of patients had significant fibrosis (F≥2). The odds ratio of F≥2 was 2.12 for the GG group and 1.54 for the CG group, compared to the CC group. The mean caloric intake was 1170±463.20 kcal/d. The odds ratio in the CC group concerning high red meat consumption in comparison to low consumption was 1.33. For white meat, the odds ratio was 0.8 when comparing high and low intake, also in the CC group. Conclusion: High red meat intake and PNPLA3 gene polymorphism seem to synergistically affect NAFLD and liver fibrosis, requiring confirmation in a larger number of patients and in different populations.

RESUMO Contexto: Estudos recentes mostram um aumento da doença hepática gordurosa não alcoólica (DHGNA) em populações com maior consumo de carne vermelha, processada e cozida em altas temperaturas. Por outro lado, o polimorfismo rs738409 no gene Patatin-like fosfolipase contendo 3 (PNPLA3) tem sido implicado na suscetibilidade à DHGNA e fibrose hepática. No entanto, o efeito sinérgico entre o consumo de carne vermelha e o polimorfismo no gene PNPLA3 na DHGNA ainda não foi avaliado. Objetivo: Avaliar a associação entre a presença do polimorfismo no gene PNPLA3 e o consumo de macronutrientes, incluindo o consumo de carne e seu modo de cozimento em pacientes com DHGNA. Métodos: Realizamos um estudo transversal com 91 pacientes diagnosticados com DHGNA por biópsia hepática e genotipados para o polimorfismo no gene PNPLA3. O consumo de calorias e macronutrientes foi verificado por meio do questionário de frequência alimentar semi-quantitativo (QFA) e do questionário específico sobre consumo de carnes. O polimorfismo no gene PNPLA3 foi analisado por reação em cadeia da polimerase em tempo real (RT-PCR) e a avaliação antropométrica foi realizada. Resultados: O índice de massa corporal médio foi de 32,38±4,58 kg/m² e a circunferência da cintura foi de 107±10 cm. Na biópsia hepática, 42% dos pacientes apresentavam fibrose significativa (F≥2). O odds ratio de F≥2 foi de 2,12 para o grupo GG e 1,54 para o grupo GC, comparado ao grupo CC. A ingestão calórica média foi de 1.170±463,20 kcal/d. O odds ratio para alto consumo de carne vermelha no grupo CC em comparação ao baixo consumo foi de 1,33. Para a carne branca, este valor foi de 0,8 ao comparar o alto e o baixo consumo, também no grupo CC. Conclusão: A alta ingestão de carne vermelha e o polimorfismo no gene PNPLA3 parecem afetar sinergicamente a DHGNA e a fibrose hepática, necessitando de confirmação em maior número de pacientes e em diferentes populações.

Abstract Introduction Post-COVID-19 syndrome is characterized by chronic fatigue and myalgia, among other symptoms, which can limit activities of daily living. Physical therapy protocols with multicomponent exercises combine strength, resistance, balance and gait, producing sig-nificant improvements in functional mobility. Objective Evaluate whether multicomponent rehabilitation is effective in improving functional mobility and quality of life in individuals with post-COVID-19 syndrome. Methods Randomized controlled trial with 59 volunteers, divided into an intervention (IG) and control group (CG), all of whom underwent initial assessment (T0). The IG performed 24 sessions over 12 weeks and the CG did not undergo training. Functional mobility was analyzed using the 6-minute walk test (6MWT) and the Berg balance scale, while the SF-36 questionnaire assessed quality of life. Results In the 6MWT, the IG covered 464.40 + 81.26 meters (T0) and 518.60 + 82.68 meters (T4). The IG Berg scale scores were 48.00 + 4.00 (T0) and 51.90 + 4.26 (T4). In terms of quality of life, the IG obtained mean scores of 96.26 + 10.14 (T0) and 102.60 + 5.53 (T4). None of these measures was statistically significant. Conclusion Individuals who underwent the multicomponent phys-iotherapy protocol showed statistically nonsignificant increases in the variables studied.

Resumo Introdução A síndrome pós-COVID-19 caracteriza-se por fadiga crônica, mialgia, entre outros sintomas que podem causar limitações na realização das atividades de vida diária. Protocolos de fisioterapia com exercício de multicomponentes combinam força, resistência, equi-líbrio e marcha, e produzem melhorias significativas na mobilidade funcional. Objetivo Avaliar se a reabilitação de multicomponentes é eficaz em promover melhora na mobilidade funcional e qualidade de vida em indivíduos com síndrome pós-COVID-19. Métodos Ensaio clínico randomizado, controlado, realizado com 59 voluntários, divididos em grupo intervenção (GI) e grupo controle (GC). Todos fizeram avaliação inicial (T0); GI realizou 24 sessões durante 12 semanas e GC não realizou treinamento. A análise da mobilidade funcional foi realizada através do teste de caminhada de 6 minutos (TC6m) e da escala de equilíbrio de Berg. O questionário SF-36 avaliou a qualidade de vida. Resultados A distância percorrida no TC6m pelo GI foi de 464,40 + 81,26 metros (T0) e de 518,60 + 82,68 metros (T4). A pontuação na escala de Berg do GI foi de 48,00 + 4,00 (T0) e de 51,90 + 4,26 (T4). Na qualidade de vida, GI apresentou escore com média de 96,26 + 10,14 (T0) e 102,60 + 5,53 (T4). Nenhuma destas medidas foram estatisticamente significantes. Conclusão Os indivíduos que foram submetidos ao protocolo fisioterapêutico de multicomponentes apresentaram incrementos nas variáveis estudadas sem significância estatística.

ABSTRACT Purpose To compare the swallowing alteration markers in patients with and without COVID-19 and to study the predictive variables of oral feeding contraindication in patients requiring prolonged orotracheal intubation. Methods Retrospective case-control study, with medical record data collection of clinical and demographic variables and the clinical evaluation of swallowing. The collected variables were statistically compared between patients with COVID-19 (SG) and without COVID-19 (CG). Robust Poisson regression analysis was used to evaluate the effect of COVID-19 and other variables on oral feeding contraindication. Results 351 patients were included, 269 in the SG and 82 in the CG. Patients in the SG were younger when compared to the CG (50.7 ± 12.8). The total time of orotracheal intubation was significantly longer in the SG. The patients in the SG had a higher prevalence of weak cough, dysphonia, worse degrees of dysphagia and higher occurrence of contraindication of oral feeding. In the bivariate analysis, it was found that patients with COVID-19 were 65% more likely to have oral feeding contraindication. However, when COVID-19 was adjusted with other clinical and demographic variables, it was found that these variables had a greater influence on the contraindication of the oral feeding than the COVID-19. Conclusion Prolonged orotracheal intubation had a worse effect on alteration markers in swallowing and reintroduction of the oral feeding in COVID-19 patients. Age over 60 years, orotracheal intubation time greater than 5 days, reintubation, and delirium were shown to be predictive of oral feeding contraindication in intubated patients.

RESUMO Objetivo comparar os marcadores de alteração na deglutição de pacientes com e sem COVID-19 e estudar as variáveis preditivas de contraindicação da alimentação por via oral em pacientes com necessidade de intubação orotraqueal prolongada. Métodos estudo caso-controle, retrospectivo, com coleta de prontuário de variáveis clínicas e demográficas e da avaliação clínica da deglutição. As variáveis coletadas foram comparadas estatisticamente entre pacientes com COVID-19 (grupo estudo -GE) e sem COVID-19 (grupo-controle - GC). A análise de regressão de robusta de Poisson foi utilizada para avaliar o efeito da COVID-19 e das demais variáveis na contraindicação da alimentação por via oral. Resultados foram incluídos 351 pacientes, 269 no GE e 82 no GC. Pacientes do GE apresentaram menor idade, quando comparados ao GC (50,7 ± 12,8). O tempo total de intubação orotraqueal foi significativamente maior no GE. Os pacientes do GE apresentaram maior prevalência de tosse fraca, disfonia, piores graus de disfagia e maior ocorrência de contraindicação da alimentação por via oral. Na análise bivariada, verificou-se que os pacientes com COVID-19 apresentaram 65% maior probabilidade dessa contraindicação. Entretanto, quando a COVID-19 foi ajustada com outras variáveis clínicas e demográficas, verificou-se que as estas apresentaram maior influência sobre a contraindicação de alimentação por via oral do que a COVID-19. Conclusão a intubação orotraqueal prolongada teve pior efeito nos marcadores de alteração na deglutição e na reintrodução da via oral de pacientes com COVID-19. A idade maior que 60 anos, tempo de intubação orotraqueal maior que cinco dias, reintubação e delirium demonstraram ser preditivas de contraindicação da alimentação por via oral em pacientes intubados.

Abstract Introduction Aging reduces functional capacity related to reduced toe plantar flexion strength. The exercise for strengthening the foot’s intrinsic muscles can be optimized using electrostimulation. Due to the scarcity of data in the literature on these methods, further studies are necessary. Objective To evaluate and compare the effects of training to strengthen the foot’s intrinsic muscles on the risk of falls in older adults. Methods This is a randomized clinical trial with 19 older patients allocated into three groups: control (CG; n = 7), exercise (EG; n = 6), and exercise+electrostimulation (EEG; n = 6). The EG received an exercise protocol for the foot’s intrinsic muscles, the EEG received the same protocol with Neuromuscular electrical stimulation (NMES), and the CG received guidance regarding preventing falls. The individuals were evaluated before and after the intervention using the Single-Limb balance test (SLBT), Functional Reach Test (FRT), Timed Up and Go (TUG), and Paper Grip Test (PGT) tests. One and two-way ANOVA was used for the statistical analysis. Statistical significance was set at p < 0.05. Results There was a significant improvement in the TUG test (9.64 ± 1.78 vs. 8.20 ± 1.94) in relation to the EG. With the EEG, there was an improvement both in the TUG (12.68 ± 4.01 vs. 10.61 ± 3.70) and in the FRT (26.37 ± 7.66 vs. 33.14±9.73) with p < 0.05). Conclusion An exercise protocol associated with electrostimulation improves performance in func-tional and dynamic balance tests in older adults.

Resumo Introdução O envelhecimento reduz a capacidade fun-cional, que está relacionada com a redução de força muscular de flexão plantar dos dedos dos pés. O exercício de fortalecimento da musculatura intrínseca do pé pode ser otimizado com o uso da eletroestimulação eletro (EENM). Devido à escassez de dados na literatura sobre a utilização desses métodos, torna-se necessário realizar novos estudos. Objetivo Avaliar e comparar os efeitos do treino de fortalecimento da musculatura intrínseca do pé no risco de queda em idosos. Métodos Trata-se de um ensaio clínico randomizado, no qual 19 idosos foram alocados em três grupos: controle (GC; n = 7), exercício (GE; n = 6) e exercício+eletroestimulação (GEE; n = 6). O GE recebeu um protocolo de exercícios para a musculatura intrínseca do pé, o GEE recebeu o mesmo protocolo seguido de EENM e o GC recebeu orientações quanto à prevenção de quedas. Os indivíduos foram avaliados antes e após a intervenção através dos testes de Apoio Unipodal (AU), Teste de Alcance Funcional (TAF), Timed Up and Go (TUG) e Paper Grip Test (PGT). Para a análise estatística, utilizou-se ANOVA 1 e 2 vias. Considerou-se estatisticamente significante um valor de p < 0,05. Resultados Observou-se melhora significativa no teste TUG (9,64 ± 1,78 vs 8,20 ± 1,94) em relação ao GE. Em relação ao GEE, houve melhora tanto no TUG (12,68 ± 4,01 vs 10,61 ± 3,70) quanto no TAF (26,37 ± 7,66 vs 33,14 ± 9,73). Conclusão Conclui-se que um protocolo de exercício associado à eletroestimulação melhora o desempenho nos testes de equilíbrio funcional e equilíbrio dinâmico em indivíduos idosos.

ABSTRACT. Unpaid caregivers of people living with dementia tend to suffer mental health problems as a result of the negative effects associated with the care tasks. Thus, psychosocial interventions for this population group are necessary. iSupport is an online support program for caregivers that was created by the World Health Organization. Objective: To describe the design of a randomized clinical trial to measure the efficacy of the iSupport-Brasil version on caregivers’ mental health and well-being. Methods: The participants will be randomized into Intervention Group (IG) (n=195) and Control Group (CG) (n=195). For three months, the IG will access the iSupport-Brasil platform, the CG will enter the electronic page of the Brazilian Alzheimer’s Association, and both groups will be emailed the preliminary version of the “Guia de cuidados para a pessoa idosa” e-book (a guide to providing care to the elderly) from the Ministry of Health. The data will be collected at three moments: baseline, and three and six months after the beginning of the intervention. Results: It is expected that it will be possible to provide diverse validity evidence about iSupport-Brasil as an online and free intervention alternative, as a preventive means and as a way to promote mental health among caregivers of people living with dementia. Conclusion: Through the evaluation protocol of this randomized clinical trial on the effects of the iSupport-Brasil program, it may become a reference for countries that plan to adapt and improve the iSupport program using digital health solutions.

RESUMO. Cuidadores informais de pessoas que vivem com demência tendem a sofrer problemas de saúde mental como resultado dos efeitos negativos associados às tarefas de cuidado. Assim, intervenções psicossociais para essa população são necessárias. O iSupport é um programa de apoio online para cuidadores criado pela Organização Mundial da Saúde. Objetivo: Descrever o desenho de um ensaio clínico randomizado para medir a eficácia da versão iSupport-Brasil na saúde mental e no bem-estar de cuidadores. Métodos: Os participantes serão randomizados em Grupo Intervenção (GI) (n=195) e Grupo Controle (GC) (n=195). Durante três meses, o GI acessará a plataforma iSupport-Brasil, o CG entrará na página eletrônica da Associação Brasileira de Alzheimer e ambos os grupos receberão por e-mail a versão preliminar do e-book Guia de cuidados para a pessoa idosa, do Ministério da Saúde. Os dados serão coletados em três momentos: linha de base, três e seis meses após o início da intervenção. Resultados: Espera-se que seja possível fornecer diversas evidências sobre os efeitos do iSupport-Brasil como alternativa de intervenção online e gratuita, de forma a promover a saúde mental entre os cuidadores de pessoas que vivem com demência. Conclusão: O protocolo de avaliação deste ensaio clínico randomizado sobre os efeitos do programa iSupport-Brasil pode se tornar uma referência para os países que planejam adaptar e melhorar esta intervenção, usando soluções digitais de saúde.

RESUMO A compactação modifica o arranjo estrutural e as funções essenciais dos poros do solo. O trabalho teve como objetivo avaliar o impacto de diferentes graus de compactação em um Argissolo Amarelo sobre os atributos físicos-hídricos indicadores da funcionalidade dos poros. Foram utilizados cilindros de solo 0.05 x 0.05 m em 4 graus de compactação (GC): 61, 71, 82 e 92%, e em cada GC, foi quantificado, sob um delineamento inteiramente aleatorizado, a distribuição de poros por tamanho, permeabilidade intrínseca do solo ao ar (Kar), índice N de continuidade de poros, curva caraterística da água no solo e frequência acumulada de poros. O incremento no GC não causou impacto na quantidade de microporos, mas diminuiu a quantidade de macroporos para valores menores que o mínimo requerido a partir do GC de 82%. O aumento no CG provocou redução no índice N, Kar e porosidade de aeração, porém com amplitude diferente dependendo do GC e da tensão da água no solo. Os GC acima de 61% reduziram o conteúdo de água na faixa de tensão entre 0 até 6 kPa e, como consequência, aumentaram a porcentagem de poros de areação; enquanto na faixa entre 10 e 1500 kPa proporcionaram maior retenção de água. A avaliação dos atributos físicos-hídricos do Argissolo Amarelo revelou que o incremento na compactação alterou a estrutura do solo, reduziu e formou poros pouco contínuos, e menos permeável ao fluxo de ar; e apesar da maior retenção de água nas tensões mais elevadas, promoveu menor conteúdo de umidade disponível.

ABSTRACT Compaction modifies the structural arrangement and essential functions of soil pores. In this context, the objective of this study was to evaluate the impact of different compaction levels in an Argissolo Amarelo (Ultisol) on the physical-hydraulic attributes that indicate the functionality of soil pores. The experiment was conducted using 0.05 x 0.05 m soil cylinders with 4 compaction levels (CL): 61, 71, 82 and 92%, and at each CL, the pore-size distribution, intrinsic soil air permeability (Kair), pore continuity index N, soil water characteristic curve and cumulative pore-size frequency were quantified under a completely randomized design. The increase in CL did not impact the amount of micropores, but reduced the amount of macropores to values lower than the minimum required from the CL of 82%. The increase in CL caused reductions in N index, Kair and aeration porosity, but with different amplitude depending on the CL and the water tension in the soil. CL above 61% reduced the water content at the tension range between 0 and 6 kPa and, as a consequence, increased the percentage of aeration pores, besides promoting greater water retention within the range between 10 and 1500 kPa. The evaluation of the physical-hydraulic attributes of the Argissolo Amarelo (Ultisol) revealed that the increase in the compaction level altered soil structure, reduced and formed pores that were poorly continuous and less permeable to air flow and, despite the higher water retention at the higher tensions, promoted lower available moisture content.

ABSTRACT The research aimed to evaluate the effect of an Oncology Physical Exercises Program - ONCOFITNESS on the joint range of cancer patients undergoing radiotherapy. The study is considered a randomized controlled clinical trial, being prospective in that it compares the effect and value of an intervention, with prophylactic or therapeutic characteristics, in human beings. The procedures met the standards for researching human beings, and the project was approved. CEP/Hospital Mário Kröeff. Flexibility measurement performed following the LABIFIE protocol, with a Lafayette® brand steel goniometer (USA). The experimental group performed an intervention with Oncofitness. After this period, another evaluation was carried out, following the same procedures. Statistical analysis was performed using SPSS (Statistical Package for the Social Sciences) version 20.0. Sample of 30 men aged GE (X ̅ = 59.0 ± 2.0 years) and CG (X ̅ = 60.0 ± 1.0 years). The data revealed that gains were observed in the EG in knee flexion (∆%=5.0%, p=0.0011x); in hip abduction (∆%=15.8%, p=0.003x); in internal rotation (∆%=8.1%, p=0.0129x) and in shoulder flexion, (∆%=8.3%, p=0.0185x). As was not observed in the CG, Oncofitness provided a reduction in some of the symptoms related to oncological treatments by improving joint range of motion.

RESUMO O objetivo da pesquisa foi avaliar o efeito de um Programa de Exercício Físico Oncológico - ONCOFITNESS na amplitude articular em pacientes com câncer submetidos à radioterapia. O estudo é um tipo de ensaio clínico prospectivo randomizado controlado no qual se compara o efeito e o valor de uma intervenção, com características profiláticas ou terapêuticas, em seres humanos. Os procedimentos consideraram as normas para realização de pesquisas em seres humanos com a aprovação do projeto pelo CEP/Hospital Mário Kröeff. A avaliação da flexibilidade foi realizada seguindo o protocolo LABIFIE, com goniômetro de aço da marca Lafayette® (EUA). O grupo experimental realizou uma intervenção com Oncofitness. Após esse período, foi realizada outra avaliação, seguindo os mesmos procedimentos. A análise estatística foi realizada no programa SPSS (Statistical Package for the Social Sciences) versão 20.0. A amostra foi composta por 30 homens com idade GE (X ̅ = 59,0 ± 2,0 anos) e GC (X ̅ = 60,0 ± 1,0 anos). Os dados revelaram que os ganhos do GE foram observados em: flexão do joelho (∆%=5,0%, p=0,0011x); em abdução do quadril (∆%=15,8%, p=0,003x); em rotação interna (∆%=8,1%, p=0,0129x) e em flexão de ombro, (∆%=8,3%, p=0,0185x). Não foi observado ganho no GC, pode-se verificar que o Oncofitness proporcionou a redução de alguns dos sintomas relacionados aos tratamentos oncológicos devido à melhora da amplitude articular.

RESUMEN El objetivo de la investigación fue evaluar el efecto de un Programa de Ejercicios Físicos Oncológicos - ONCOFITNESS en la amplitud articular en pacientes oncológicos sometidos a radioterapia. El estudio es tipo ensayo clínico controlado randomizado, prospectivo en que compara el efecto y valor de una intervención, con características profilácticas o terapéuticas, en seres humanos. Los procedimientos consideraron las normas para la realización de investigación en seres humanos con la aprobación del proyecto por el CEP/Hospital Mário Kröeff. La evaluación de la flexibilidad fue realizada siguiendo el protocolo del LABIFIE, con un goniómetro de acero da marca Lafayette® (EUA). El grupo experimental realizó una intervención con el Oncofitness. Después de ese periodo, se realizó otra evaluación, siguiendo los mismos procedimientos. El análisis estadístico fue realizado mediante el SPSS (Statistical Package for the Social Sciences) versión 20.0. La muestra fue de 30 hombres con edades GE (X ̅ = 59,0 ± 2,0 años) y GC (X ̅ = 60,0 ± 1,0 años). Los datos revelaron que fueron observadas ganancias GE en: flexión de rodillas (∆%=5,0%, p=0,0011x); en la abducción de cadera (∆%=15,8%, p=0,003x); en la rotación interna (∆%=8,1%, p=0,0129x) y en flexión de hombro, (∆%=8,3%, p=0,0185x). No fue observada ganancia en el GC, se puede verificar que el Oncofitness proporcionó la reducción de algunos de los síntomas relacionados a los tratamientos oncológicos por la mejora de la amplitud articular.

Abstract Introduction and objectives: Alpha2 adrenergic agonists, such as clonidine, are used as adjuvants during anesthesia due to their analgesic, sedative, and cardiovascular effects. The objective of the present study was to compare the effect of clonidine administered intravenously and intrathecally on the postoperative pain score of patients undergoing laparoscopic cholecystectomy, according to the route of administration and postoperative opioid consumption. Methods: This randomized clinical trial, blind to patients and evaluator, assessed 60 patients, candidates for elective laparoscopic cholecystectomy under standardized general anesthesia techniques. Patients were randomly allocated into three groups (20 in each group): Control Group (CG), Intrathecal Clonidine Group (ITCG), and Intravenous Clonidine Group (IVCG). The primary outcome was the comparison of pain, Blood Pressure (BP) and Heart Rate (HR) scores among groups. The secondary outcome was report of adverse effects such as bradycardia, hypotension and sedation, and the need for rescue medication. Results: The mean age was 37.2 ± 8.2 years, and the mean body mass was 28.3 ± 3.6 kg.m-2. Regarding HR (p = 0.003) and pain (p = 0.027), patients in ITCG and CG showed a different profile over time, with CG showing higher consumption of morphine as rescue medication (p = 0.005). Conclusion: The administration of intrathecal and intravenous clonidine in low doses can reduce hemodynamic parameters and decrease postoperative requirement of analgesics. Further studies should investigate the ideal dose and method.

ABSTRACT The effect of different calcium levels and two limestone granulometries on performance, egg quality, biometry of digestive organs and bone characteristics of light laying hens were evaluated. A total of 270 laying hens were used during 112 days, distributed in a completely randomized design in a 3 x 2 factorial scheme, with 3 levels of calcium (3.8, 4.0 and 4.2%) and 2 limestone granulometries (0.222 and 1.922 mm), totaling 6 treatments with 5 repetitions each. The parameters evaluated were: feed intake, egg production, egg weight and mass, feed conversion per mass and per dozen eggs, albumen, yolk and shell percentage, specific gravity of eggs and shell thickness. In addition, the relative weight of the heart, proventricle, gizzard, liver, pancreas, intestine weight and length, and bone characteristics were also evaluated. A significant interaction was observed for tibia mineral matter. Egg production was influenced by the limestone granulometry, presenting greater value in the treatments which contained fine granulometry limestone. Higher calcium levels of 4.0 and 4.2% provide greater specific density of eggs. Biometric variables and bone characteristics were not influenced by the treatments. In conclusion, it is recommended to use fine-grained limestone (0.222 mm) and a 4% calcium level in diets for light commercial hens, as they improve performance and quality of eggs, without interfering in biometrics of digestive organs and bone characteristics.

Aim To evaluate the influence of the parameters L* a* b* on the variation of the color of bovine tooth enamel submitted to artificial darkening, after simulated brushing, with whitening toothpastes containing blue covarine. Methods To undertake this study in vitro, 60 specimens (SP) were divided into 6 groups (n=10): control group (CGwater) and 5 test groups (GT1-Colgate Total 12, GT2-Oral-B 3D White Perfection, GT3-Colgate Luminous Instant White, GT4-CloseUp White Diamond Attraction, GT5-Sorriso Xtreme White). The specimens were darkened with coffee and submitted to simulated brushing for 6, 12, and 24 months. The alteration in the color was evaluated using CIELAB parameters and the ∆L, ∆a, ∆b and ∆E were calculated. The data was analyzed through generalized linear models using the R program and considering a level of significance of 5%. Results The parameters L*, a* and the ∆L, ∆a obtained better results in the test group than in the control group. There were no statistical differences between CG and the test groups for the evaluation of the b* parameter. In the evaluation of the ∆b, the GT3 differed statistically from the CG. In relation to the ∆E, all the group tests showed a variation in color statistically greater than that of the CG and the GT4 showed the greatest variation, not differing from the GT3 during the periods studied. Conclusion The mechanical and optical whitening agents positively influenced the values L*a* and b*, as well as in its variations and in the ∆E. It is important to emphasize, however, that to analyze tooth whitening it is necessary to evaluate their parameters together.

RESUMO Introdução: a fluência de leitura é uma medida capaz de avaliar de forma objetiva o desempenho de escolares em leitura oral. Objetivo: avaliar a utilização da fluência de leitura como um indicador de competência leitora a ser utilizado na educação inclusiva. Métodos: participaram do estudo 121 escolares do 3° ao 5° ano do ensino fundamental, destes 31 escolares do público-alvo da educação especial compuseram o grupo de interesse e 90 escolares compuseram o grupo controle. Foi definido um escore Z que permitiu a realização da análise individual do desempenho dos escolares e a identificação dos possíveis déficits nas tarefas de fluência de leitura e compreensão, foi aplicado o Test t de Student para comparação dos grupos. Resultados: houve variabilidade nos desempenhos apresentados por cada subgrupo do público-alvo da educação especial. Na comparação dos dois grupos, os resultados foram superiores para o GC em todas as variáveis. Conclusão: A diversidade encontrada dentro dos subgrupos do público-alvo da educação especial mostrou que a avaliação de fluência de leitura individual é essencial tanto para pesquisas quanto para a prática pedagógica.

ABSTRACT Introduction: reading fluency is a measure capable of objectively evaluating the performance of students in oral reading. Objective: to evaluate the use of reading fluency as an indicator of reading competence to be used in inclusive education. Methods: 121 students from the 3rd to the 5th year of elementary school participated in the study. 31 of these students from the target group of special education were in the interest group and 90 students were in the control group. A Z score was defined to allow for the individual analysis of the students’ performance and the identification of possible deficits in the tasks of fluency in reading and comprehension. The Student’s t-test was carried out to compare the groups. Results: There was variability in the performances presented by each subgroup of the target group of special education. When comparing the two groups, the results were superior for the CG in all variables. Conclusion: The diversity found within the subgroups of the target audience of special education showed that the assessment of individual reading fluency is essential for both research and pedagogical practice.

Resumo Introdução: Com o envelhecimento, é comum ocorrerem alterações em diferentes áreas da cognição, como a memória, função executiva, linguagem, desenvolvimento psicomotor e função visoespacial. A atividade física regular, contudo, tem sido descrita como um excelente meio de atenuar as degenerações provocadas pelo envelhecimento dentro dos domínios físico, psicológico e social. Objetivo: Avaliar os efeitos do treinamento resistido em idosas com comprometimento cognitivo leve. Métodos: Estudo experimental com 31 idosas sedentárias, divididas em grupo controle (GC; n = 15) e grupo treinamento resistido (GTR; n = 16), submetidas a avaliações antropométricas, composição corporal, força máxima, frequência cardíaca (FC), pressão arterial (PA) e aplicação de questionário (Mini Exame do Estado Mental). Resultados: Observou-se aumento da capacidade cognitiva no GTR (pós 26,00 ± 2,13 vs. GC 22,24 ± 3,82 vs. pré 24,06 ± 2,38 GTR) e redução na PA sistólica (pós 107,50 ± 11,97 vs. GC 126,00 ± 9,72 vs. pré 124,13 ± 12,55 mmHg GTR), PA diastólica (pós 68,50 ± 8,15 vs. GC 81,73 ± 4,59 vs. pré 74,69 ± 6,87 mmHg GTR) e duplo produto no GTR (pós 7746 ± 1244 vs. GC 9336 ± 1595 vs. pré 9286 ± 1309 mmHg x bpm GTR). Não houve redução na FC no GTR (pós 72,00 ± 7,40 vs. GC 74,00 ± 10,50 vs. pré 74,94 ± 8,42 bpm GTR). Em relação à força muscular, observou-se aumento em todos os exercícios. Conclusão: O presente estudo mostrou que o treinamento resistido aumentou a força muscular e que houve redução de variáveis hemodinâmicas; entretanto, o achado mais importante desse estudo foi o aumento na capacidade cognitiva das idosas.

Abstract Introduction: With aging, it is common for some changes to occur in different areas of cognition, such as memory, executive function, language and psychomotor speed. However, regular physical activity has been described as an excellent way to alleviate the degeneration caused by aging within the various physical, psychological and social domains. Objective: To evaluate the effects of resistance training in elderly women with mild cognitive impairment. Methods: Experimental study with 31 sedentary elderly women divided into control (CG; n=15) and resistance training (RTG; n=16) groups who underwent assessment of anthropometric measures, body composition, maximum strength, heart rate and blood pressure and questionnaire application (Mini-Mental State Examination). Results: Cognitive ability increased in RTG (post 26.00 ± 2.13 vs. CG 22.24 ± 3.82 vs. pre 24.06 ± 2.38 RTG). In RTG, there was a reduction in systolic blood pressure (post 107.50 ± 11.97 vs. CG 126.00 ± 9.72 vs. pre 124.13 ± 12.55 mmHg RTG), diastolic blood pressure (post 68.50 ± 8.15 vs. CG 81.73 ± 4.59 vs. pre 74.69 ± 6.87 mmHg RTG) and double product (post 7746 ± 1244 vs. CG 9336 ± 1595 vs. pre 9286 ± 1309 mmHg x bpm RTG), but not in heart rate (post 72.00 ± 7.40 vs. CG 74.00 ± 10.50 vs. pre 74.94 ± 8.42 bpm RTG). Regarding muscle strength, an increase was evident in all exercises. Conclusion: The present study showed that resistance training in elderly women increased muscle strength and reduced hemodynamic variables. But the most important finding was that there was an increase in cognitive capacity.

The goal of the present study was to compare pulmonary function test (PFT) and cardiopulmonary exercise test (CPET) performance in COVID-19 survivors with a control group (CG). This was a cross-sectional study. Patients diagnosed with COVID-19, without severe signs and symptoms, were evaluated one month after the infection. Healthy volunteers matched for sex and age constituted the control group. All volunteers underwent the following assessments: i) clinical evaluation, ii) PTF; and iii) CPET on a cycle ergometer. Metabolic variables were measured by the CareFusion Oxycon Mobile device. In addition, heart rate responses, peak systolic and diastolic blood pressure, and perceived exertion were recorded. Twenty-nine patients with COVID-19 and 18 healthy control subjects were evaluated. Surviving patients of COVID-19 had a mean age of 40 years and had higher body mass index and persistent symptoms compared to the CG (P<0.05), but patients with COVID-19 had more comorbidities, number of medications, and greater impairment of lung function (P<0.05). Regarding CPET, patients surviving COVID-19 had reduced peak workload, oxygen uptake (V̇O2), carbon dioxide output (V̇CO2), circulatory power (CP), and end-tidal pressure for carbon dioxide (PETCO2) (P<0.05). Additionally, survivors had depressed chronotropic and ventilatory responses, low peak oxygen saturation, and greater muscle fatigue (P<0.05) compared to CG. Despite not showing signs and symptoms of severe disease during infection, adult survivors had losses of lung function and cardiorespiratory capacity one month after recovery from COVID-19. In addition, cardiovascular, ventilatory, and lower limb fatigue responses were the main exercise limitations.

The intracranial compliance in type 2 diabetes mellitus (T2DM) patients and the association with cardiovascular autonomic control have not been fully elucidated. The aim of this study was to assess intracranial compliance using the noninvasive intracranial pressure (niICP) and the monitoring of waveform peaks (P1, P2, and P3) and the relationship with cardiovascular autonomic control in T2DM patients. Thirty-two men aged 40-60 years without cardiovascular autonomic neuropathy (CAN) were studied: T2DMG (n=16) and control group CG (n=16). The niICP was evaluated by a noninvasive extracranial sensor placed on the scalp. Cardiovascular autonomic control was evaluated by indices of the baroreflex sensitivity (BRS), from temporal series of R-R intervals of electrocardiogram and systolic arterial pressure, during supine and orthostatic positions. The participants remained in the supine position for 15 min and then 15 min more in orthostatism. T2DMG presented a decrease of the P2/P1 ratio during the orthostatic position (P<0.001). There was a negative moderate correlation between the P2 peak with cardiovascular coupling (K2HP-SAPLF) in supine (r=-0.612, P=0.011) and orthostatic (r=-0.568, P=0.020) positions in T2DMG. We concluded that T2DM patients without CAN and cardiovascular complications presented intracranial compliance similar to healthy subjects. Despite preserved intracranial adjustments, T2DM patients had a response of greater magnitude in orthostatism. In addition, the decoupling between the heart period and blood pressure signal oscillations in low frequency appeared to be related to the worsening of intracranial compliance due to the increased P2 peak.