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Green Synthesis, Characterization and Antibacterial and Leishmanicidal Activities of Silver Nanoparticles Obtained from Aqueous Extract of Eucalyptus grandis
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Oliveira, Lucas M. F.

; Silva, Ueveton P. da

; Braga, João Pedro V.

; Teixeira, Álvaro V. N. C.

; Ribon, Andréa O. B.

; Varejão, Eduardo V. V.

; Coelho, Eduardo A. F.

; Freitas, Camila S. de

; Teixeira, Róbson R.

; Moreira, Renata P L.

Journal of the Brazilian Chemical Society

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Abr 2023, Volume 34 Nº 4 Páginas 527 - 536

Resumo: En | Texto: En | PDF: En

This work describes a green synthesis, the characterization, and biological evaluation of silver nanoparticles (AgNPs). The AgNPs suspension was synthesized using aqueous leaf extract of Eucalyptus grandis, which presented a characteristic band at 407 nm in the UV-Vis spectrum. The AgNPs presented a spherical shape and size of 9.7 ± 0.3 nm. The nanoparticles were stable over a month, indicating that E. grandis’ extract is suitable for their preparation and stabilization. The X-ray analysis showed that the crystallinity of AgNPs corresponded to the centered face phase of silver. The antibacterial and leishmanicidal activities of AgNPs were evaluated. The AgNPs presented antibacterial activity on the Gram-negative bacteria Escherichia coli at 53.9 µg mL-1. The leishmanicidal activity evaluation against promastigote forms of Leishmania infantum, Leishmania amazonensis, and Leishmania braziliensis showed that the biological response is dependent on the volume of AgNP suspension. It was demonstrated that L. infantum was more sensitive to the nanoparticle’s treatment than L. amazonensis and L. braziliensis. The treatment of L infantum promastigotes with 150 µL of AgNP suspension reduced parasite growth by 67.9%, a result which was similar to the treatment with 1 (66.7%) or 2 µL (70.6%) of amphotericin B used as a positive control.

https://doi.org/10.21577/0103-5053.20220126

Synthesis and Evaluation of the Antileishmanial Activity of Novel Eugenol Analogs Containing 1,2,3-Triazole Fragments against Intracellular Leishmania braziliensis
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Evangelista, Roberta S.

; Pereira, Larissa C.

; Souza, Luciana Â. de

; Costa, Adilson V.

; Silva, Danilo A. da

; Oliveira, Fabrício M. de

; Vaz, Boniek G.

; Bressan, Gustavo C.

; Fietto, Juliana L. R.

; Teixeira, Róbson R.

Journal of the Brazilian Chemical Society

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2023, Volume 34 Nº 12 Páginas 1810 - 1824

Resumo: En | Texto: En | PDF: En

This investigation describes the synthesis of eugenol analogs presenting 1,2,3-triazole fragments and evaluation of their antileishmanial activity. The alkylation of guaiacol (1) with allyl bromide afforded 1-(allyloxy)-2-methoxybenzene (2) (93% yield). The Claisen rearrangement conducted with 1 gave ortho eugenol (3) (82% yield). Alkylation procedures performed with 3 produced 1-allyl-3-methoxy-2-(prop-2-yn-1-yloxy)benzene (4) (73% yield) and 1-allyl-3-methoxy-2-(pent-4-yn-1-yloxy)benzene (6) (53% yield). The copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions involving alkynes 4 and 6 with different benzylic azides afforded twenty-two eugenol analogs with 1,2,3-triazole functionalities (48-93% yield). We screened the compounds at 10 μmol L 1 against Leishmania braziliensis intracellular amastigotes during macrophage infection. The action of these compounds was compared with the known leishmanicidal drug amphotericin B. None of the analogs were toxic to macrophages at 10 μmol L-1. The cytotoxic concentration at 50% (CC50), effective concentration at 50% (EC50), and selectivity index (SI) were determined to the best compounds 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-chlorobenzyl)-1 H-1,2,3-triazole (8c) and 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-trifluoromethoxybenzyl)-1 H-1,2,3-triazole (8h). They showed a significant leishmanicidal effect, with EC50 of 28.09 µmol L-1 (8c) and 52.03 µmol L-1 (8h). The SIs were 9.7 for 8c and > 5.7 for 8h. These compounds have the potential as new leishmanicidal agents against L. braziliensis and may represent a starting point for the development of alternative treatments for cutaneous leishmaniasis.

https://doi.org/10.21577/0103-5053.20230073

Synthesis of Novel Cinnamides and a Bis Cinnamate Bearing 1,2,3-Triazole Functionalities with Antiproliferative and Antimetastatic Activities on Melanoma Cells
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Santos, Fabíola S

; Vale, Juliana A. do

; Santos, Lucas S

; Gontijo, Talita B

; Lima, Graziela D. A

; Oliveira, Leandro L. de

; Machado-Neves, Mariana

; Teixeira, Róbson R

; Freitas, Rossimiriam P. de

Journal of the Brazilian Chemical Society

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Dez 2021, Volume 32 Nº 12 Páginas 2174 - 2185

Resumo: En | Texto: En | PDF: En

The present investigation describes the synthesis of novel cinnamides and a bis cinnamate bearing 1,2,3-triazole functionalities and investigation of their antiproliferative and antimetastatic effects on melanoma cells. The necessity for the development of new chemotherapeutic agents for melanoma treatment motivated this work. Sixteen derivatives were obtained with yields ranging from 23-81% and fully characterized by spectroscopic (1H and 13C nuclear magnetic resonance, infrared) and spectrometric high resolution mass spectrometry (HRMS) techniques. The derivatives were in vitro evaluated against B16-F10 murine melanoma cell line. The most effective compound (a bis cinnamate) (6b) reduced the melanoma cell viability, generated cell cycle arrest, and influenced the metastatic behavior of melanoma cells by decreasing migration, invasion, and colony formation. Based on these findings, it is believed that compound 6b may represent an interesting scaffold to be explored toward the development of new antimelanoma agents.

https://doi.org/10.21577/0103-5053.20210109

Nematodes of Proceratophrys ararype (Anura: Odontophrynidae), an endemic frog from the Araripe Plateau, northeastern Brazil
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Mascarenhas, Wilmara

; Oliveira, Cicero R.

; Benício, Ronildo A.

; Ávila, Robson W.

; Ribeiro, Samuel C.

Biota Neotropica

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2021, Volume 21 Nº 3 elocation e20201164

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: Os parasitas são um componente importante da biomassa global, tendo papel significativo em vários mecanismos reguladores no ecossistema. O parasitismo é uma das interações ecológicas mais comuns no planeta. Estudos demonstraram que somente 8% da fauna de helmintos das espécies de anfíbios que ocorrem no Brasil foi estudada, sendo esta porcentagem mais baixa para a família Odontophrynidae, tendo sido investigadas apenas quatro das 50 espécies com ocorrência conhecida para o Brasil. Aqui, apresentamos a helmintofauna de Proceratophrys ararype, um anuro endêmico do “Brejo de Altitude” Chapada do Araripe, nordeste do Brasil. Os parâmetros de infecção analisados foram prevalência, intensidade média de infecção e abundância média de parasitas. Utilizamos o coeficiente de correlação linear de Pearson para verificar as correlações entre a abundância dos parasitas com o comprimento rostro-cloacal dos hospedeiros. Para verificar o grau de agregação dos parasitas nos hospedeiros, utilizamos o índice de dispersão. Dos 40 espécimes examinados, 19 estavam infectados com pelo menos um parasita. A prevalência geral foi de 47.5% com intensidade média de infecção de 18.93 ± 10.77. A comunidade endoparasitária associada a P. ararype constituiu de seis espécies de parasitas, com Falcaustra mascula tendo a maior prevalência (25%). A maioria dos parasitas apresentaram índice de dispersão uniforme nos hospedeiros e sua abundância não esteve relacionada ao tamanho do hospedeiro. Aqui, nós também apresentamos uma compilação de todos os parasitas associados as espécies de hospedeiros do gênero Proceratophrys na América do Sul. No geral, nós encontramos 23 espécies de parasitas associadas a cinco espécies de hospedeiros (P. ararype, P. appendiculata, P. boiei, P. cristiceps, P. mantiqueira). Destas, duas espécies de parasitas (Oswaldocruzia mazzai e Strongyloides sp.) representam novos registros para o gênero Proceratophrys. Nossos resultados demonstram a carência de estudos sobre a helmintofauna de anfíbios e preenchem uma importante lacuna de conhecimento sobre a diversidade de parasitas de Proceratophrys ararype, um sapo endêmico do Planalto do Araripe, nordeste do Brasil.

Abstract: Parasites are an important component of the global biomass, having significant roles in several regulatory mechanisms in the ecosystem. Parasitism is one of the most common ecological interactions on the planet. Studies have shown that the helminth fauna of only 8% of amphibian species in Brazil have been studied, and this percentage is lower for the Odontophrynidae family, with only four of the 50 species kown to occur in Brazil having been investigated. Here, we present the helminth fauna of Proceratophrys ararype, an anuran endemic to the “Brejo de Altitude” Chapada do Araripe (Araripe Plateau), northeastern Brazil. The infection parameters analyzed were prevalence, mean intensity of infection and mean abundance of parasites. We used the Pearson’s linear correlation coefficient to check the correlations between the abundance of the parasites with the snout-vent length (SVL) of hosts. To verify the degree of aggregation of parasites in hosts, we used the dispersion index. Out of 40 specimens examined, 19 specimens were infected with at least one parasite. The overall prevalence was 47.5% with a mean infection intensity of 18.93 ± 10.77. The endoparasitic community associated with P. ararype consisted of six species of parasites, with Falcaustra mascula having the highest prevalence (25%). Most parasites had a uniform dispersion index in the hosts and their abundance was unrelated to host size. Here, we also present a compilation of all parasites associated with host species of the genus Proceratophrys from South America. Overall, we found 23 species of parasites associated with five host species (P. ararype, P. appendiculata, P. boiei, P. cristiceps, P. mantiqueira). Of these, two species of parasites (Oswaldocruzia mazzai and Strongyloides sp.) represent new records for the genus Proceratophrys. Our results demonstrate the lack of studies on amphibian helminth fauna and fill an important knowledge gap on the diversity of parasites of Proceratophrys ararype, an endemic frog from the Araripe Plateau, northeastern Brazil.

https://doi.org/10.1590/1676-0611-bn-2020-1164 115 downloads

Emergence and growth of maize submitted to inoculant doses associated with saline water irrigation
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Oliveira, Hallyson

; Nascimento, Ronaldo do

; Nascimento, Elka Costa Santos

; Lima, Robson Felipe de

; Bezerra, Carlos Vailan de Castro

Pesquisa Agropecuária Tropical

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2020, Volume 50 elocation e66102

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO olvimento das plantas, tornando-se uma problemática limitante à produção agrícola. Objetivou-se avaliar o efeito de doses de inoculante de Azospirillum brasilense na emergência e crescimento de milho submetido a concentrações de salinidade da água de irrigação. O experimento foi desenvolvido em casa-de-vegetação, com delineamento experimental de blocos casualizados, fatorial 3 x 5 [doses de A. brasilense (0.0; 0.32; e 0.48 mL/100 sementes) e níveis de condutividade elétrica da água (0.3; 0.6; 1.1; 1.7; e 2.3 dS m-1)], em 4 blocos, totalizando 60 unidades experimentais. Foram avaliados a porcentagem de emergência, índice de velocidade de emergência, altura de planta, diâmetro de colmo, número de folhas, área foliar e massa seca de folhas, colmo, pendão e raízes. Os tratamentos entre salinidade e inoculação não apresentaram efeito sobre a emergência das plântulas. A salinidade afetou significativamente o crescimento; porém, o número de folhas apresentou acréscimos de 12.8 % (V8) e 18.8 % (V10), comparando-se a diferença entre a menor e maior salinidade estudada. Quanto à altura de planta, houve incremento de 3,7 % até a salinidade limiar da cultura (1.1 dS m-1). Ocorreu aumento de massa seca das raízes com a aplicação das doses de inoculante em cada nível salino estudado.

ABSTRACT Salinity, in general, affects the plant growth and development, making it a limiting problem for the agricultural production. This study aimed to evaluate the effect of inoculant doses of Azospirillum brasilense on the emergence and growth of maize submitted to salinity concentrations of the irrigation water. The experiment was carried out in a greenhouse, with a randomized blocks design, in a 3 x 5 factorial [A. brasilense doses (0.0, 0.32 and 0.48 mL/100 seeds) and levels of electrical conductivity of the water (0.3, 0.6, 1.1, 1.7 and 2.3 dS m-1)], in 4 blocks, totaling 60 experimental units. The emergence percentage, emergence speed index, plant height, stem diameter, number of leaves, leaf area, leaf dry mass, stem dry mass, tassel dry mass and root dry mass were evaluated. The treatments between salinity and inoculation had no effect on seedling emergence. The salinity significantly affected growth; however, the number of leaves increased in 12.8 % (V8) and 18.8 % (V10), when comparing the difference between the lowest and highest studied salinity. As for the plant height, there was an increase of 3.7 % up to the threshold salinity of the crop (1.1 dS m-1). There was an increase in the root dry mass with the application of inoculant doses at each studied salt level.

https://doi.org/10.1590/1983-40632020v5066102 136 downloads

Effect of Arrabidaea chica extract against chemically induced breast cancer in animal model
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Rocha, Keyla Borges Ferreira

; Oliveira, Cláudia Nunes

; Azevedo, Ítalo Medeiros

; Macedo, Robson de

; Medeiros, Aldo Cunha

Acta Cirúrgica Brasileira

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Out 2019, Volume 34 Nº 10 elocation e201901001

Resumo: En | Texto: En | PDF: En

Abstract Purpose: To examine the effects of Arrabidaa chica (Bignoniacea) extract, a native plant of the Amazon known as crajiru, on a 7,12-dimethyl-1,2-benzanthracene (DMBA)-induced breast cancer model in Wistar rats. Methods: We compared the response of breast cancer to the oral administration of A. chica extract (ACE) for 16 weeks, associated or not with vincristine. Groups: normal control; DMBA (50mg/kg v.o,) without treatment; DMBA+ACE (300 mg/kg); DMBA+vincristine. 500μg/kg injected i.p; DMBA+ACE+Vincristine 250μg/kg i.p. Imaging by microPET and fluorescence, biochemistry, oxidative stress, hematology and histopathology were used to validate the treatments. Results: All animals survived. A gradual weight gain in all groups was observed, with no significant difference (p>0.05). The oral administration of ACE and ACE+vincristine 50% significantly reduced breast tumors incidence examined with PET-18FDG and fluorescence (p<0.001). Significant reduction of serum transaminases, oxidative stress and hematological toxicity were observed in these groups. Antioxidant enzyme levels in breast tissue were significantly higher compared to the DMBA and DMBA+vincristine groups. Conclusion: These results demonstrate for the first time that ACE positively influences the treatment of DMBA-induced breast cancer in animal model, inducing a reduction in oxidative stress and chemotherapy toxicity, meaning that ACE may have clinical implication in further studies.

https://doi.org/10.1590/s0102-865020190100000001 1258 downloads

Thermal comfort monitoring in aviaries by a real-time data acquisition system
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Camargo, Tiago F. B.

; Silva, Robson L.

; Higa, Márcio

; Coutinho, Mônica R.

; Oliveira, Júlio C. D. de

; Conceição, Wagner A. dos S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Set 2019, Volume 23 Nº 9 Páginas 694 - 701

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O conforto térmico no interior de instalações de criação de frangos de corte é essencial na obtenção de bons resultados nesta atividade de produção. A avaliação das condições termodinâmicas adequadas requer a medição e controle, geralmente implicando em custos e manutenção especializada. Neste trabalho, objetivou-se monitorar a distribuição de temperatura, umidade relativa e velocidade do ar utilizando sistema desenvolvido, de baixo custo, de código aberto e de fácil uso, com Arduíno (hardware) e Scilab (software) para aquisição de dados em tempo real. Os sensores foram instalados em uma unidade de produção existente (Cianorte, PR), com medições para o ambiente interno (20 sensores de temperatura/umidade e 2 sensores de pressão diferencial, respectivamente, a 0,5 e 1,5 m de altura) e para ambiente externo (pressão e velocidade do vento, a 1,5 m de altura). Sistema de aquisição de dados permitiu a comunicação com sensores, que são facilmente lidos por computador e armazenados em um arquivo de dados. O sistema de aquisição de dados desenvolvido provou-se eficiente quando aplicado em uma instalação de criação comercial de frangos de corte, permitindo o monitoramento em tempo real para os parâmetros de conforto térmico.

ABSTRACT Thermal comfort inside broiler husbandry facilities is essential in obtaining good results in the production activity. Assessment of adequate thermodynamic conditions requires measurement and control, usually implying costs and specialized maintenance. The objective of this research was to monitor the temperature, relative humidity and air speed distributions by a developed low-cost, open-source and easy-to-use measurement system, using Arduino (hardware) and Scilab (software) for real-time data acquisition. Sensors were installed in a real facility (Cianorte, PR), with measurements for internal ambient (20 sensors for temperature/relative humidity, and two sensors for differential pressure, respectively 0.5 and 1.5 m high) and external ambient (pressure and wind speed, 1.5 m high). Data acquisition system has enabled communication with sensors which are easily read by the computer and stored in a data file. The developed data acquisition system proved to be efficient when applied in a commercial broiler husbandry facility, enabling real-time monitoring for thermal comfort parameters.

https://doi.org/10.1590/1807-1929/agriambi.v23n9p694-701 1331 downloads

SÍNTESE E AVALIAÇÃO DAS ATIVIDADES FOTOPROTETORA, CITOTÓXICA E ANTIVIRAL CONTRA O ZIKA VÍRUS DE DERIVADOS TRIAZÓLICOS DA BENZOFENONA
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Lima, Ângela M. A.

; Teixeira, Róbson R.

; Silva, Bianca F. da

; Siqueira, Raoni P.

; Silva, Ítalo E. P. da

; Santos, Edjon G.

; Fernandes, Maria Cecília

; Gonçalves, Victor Hugo Sousa

; Bressan, Gustavo C.

; Mendes, Tiago Antonio de Oliveira

; Paula, Sérgio O. de

; Costa, Adílson Vidal

; Santos, Marcelo H. dos

Química Nova

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Mai 2019, Volume 42 Nº 5 Páginas 473 - 484

Resumo: Pt | Texto: Pt | PDF: Pt

The benzophenones are synthetic and natural compounds presenting a variety of activities, including photoprotective, cytotoxic and antiviral. It is herein described the preparation of a series of twenty-seven benzophenone derivatives bearing 1,2,3-triazole functionalities and the evaluation of their photoprotective, cytotoxic and antiviral on Zika Virus (ZIKV) activities. The compounds were prepared in three steps, namely reduction of benzophenone, alkylation of diphenylmethanol and CuAAC reactions. The in vitro evaluation of the photoprotective activity revealed that the most active derivative 4-((benzhydryloxy)methyl)-1-(4-nitrobenzyl)-1H-1,2,3-triazole (4k) displayed UVB sun protection factor equal to 6,9±0,53, which make this compound a possible candidate to be used in formulations for photoprotective applications. In terms of cytotoxicity, the compounds were evaluated against MDA-MB-231 and B16F10 cell lines. It was observed that the compounds were more active against MDA-MB-231 cells and three of them were capable of reducing cell viability by approximately 55% at 100 µmol L-1. In the antiviral screening against ZIKV, compound 4-(3-benzhydryloxy)propyl)-1-(3-methylbenzyl)-1H-1,2,3-triazole (5j) was the most effective in mantaining Vero cell viability.

https://doi.org/10.21577/0100-4042.20170365 1170 downloads

Mitigation of Mombasa Grass ( Megathyrsus maximus ) Dependence on Nitrogen Fertilization as a Function of Inoculation with Azospirillum brasilense
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Leite, Rubson da Costa

; Santos, Antonio Clementino dos

; Santos, José Geraldo Donizetti dos

; Leite, Robson da Costa

; Oliveira, Leonardo Bernardes Taverny de

; Hungria, Mariangela

Revista Brasileira de Ciência do Solo

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2019, Volume 43 elocation e0180234

Resumo: En | Texto: En | PDF: En

ABSTRACT Using biological inputs to improve the efficiency of nitrogen fertilizers represents an alternative for the cultivation of grasses in tropical regions. Azospirillum brasilense is a species of plant growth promoting bacteria widely studied and used in inoculants. Thus, this study aimed to evaluate the performance of Mombasa grass ( Megathyrsus maximus ) in association with A. brasilense and nitrogen (N) fertilization. The study was conducted under field conditions in Araguaína-Tocantins State, between December 2017 and May 2018. The treatments were arranged in randomized blocks, in a 5 × 2 factorial arrangement, with five doses of N fertilization (0, 25, 50, 75, and 100 kg ha-1) combined with two inoculation treatments (inoculated and non inoculated), in four replicates. For the number of tillers and root production, the inoculation efficiency varied as a function of the supplied N doses. However, the percentage of leaf N was higher for inoculated plants regardless of the application of nitrogen. In the absence of nitrogen fertilization, it was possible to increase forage production by up to 36 % with inoculation.

https://doi.org/10.1590/18069657rbcs20180234 1669 downloads

10.

Hematological and biometric traits of tuvira Gymnotus inaequilabiatus (Valenciennes, 1839) (Gymnotiformes: Gymnotidae) from the Brazilian Pantanal
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RODRIGUES, ROBSON A.

; SILVA, EDUARDA S.

; MARCONDES, SANDRIELY F.

; GALINDO, GIZELA M.

; OLIVEIRA, GUSTAVO G. DE

; SOUZA, ALDA I. DE

; RAGUSA-NETTO, JOSÉ

; FERNANDES, CARLOS E.

Anais da Academia Brasileira de Ciências

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Mar 2018, Volume 90 Nº 1 Páginas 49 - 57

Resumo: En | Texto: En | PDF: En

ABSTRACT This study describes the hematological and biometric characteristics of male and female Gymnotus species from the Pantanal, Mato Grosso do Sul state, Brazil. Fifty adult specimens of Gymnotus inaequilabiatus were weighed, measured, and then euthanized. Blood was collected by puncturing the celiac mesenteric vein to determine the hematocrit, hemoglobin content, number of erythrocytes, mean corpuscular volume, mean corpuscular hemoglobin concentration, glucose level, absolute value of leukocytes, and relative value of leukocytes and thrombocytes. Body weight and relative condition factor did not differ (P > 0.05) between the sexes, as well as erythrogram and the blood glucose values. Hematocrit ranged from 18.0% to 54.0%; hemoglobin from 1.1 to 14.7 g dL-1; number of erythrocytes from 0.2 × 106 to 3.8 ×106 µL-1; MCV from 24.2 to 321.7 fL; and MCHC from 4.2 to 44.5 g dL-1. In the differential count were identified thrombocytes, lymphocytes, neutrophils, monocytes, basophils, immature leukocytes, and PAS-positive granular leukocyte (PAS-GL). Females had a higher percentage of immature leukocytes (P < 0.05) than males. Glucose levels, erythrogram, leukogram, and the morphology of defense cells are comparable to other fish species of the Pantanal. Thrombocytes were the most frequent defense cells, followed by lymphocytes and neutrophils.

https://doi.org/10.1590/0001-3765201720150824 1048 downloads

11.

Facile Synthesis of Tellurium Nanowires and Study of Their Third-Order Nonlinear Optical Properties
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Silva, Robson R.

; Mejia, Hans A. G.

; Ribeiro, Sidney J. L.

; Shrestha, Lok K.

; Ariga, Katsuhiko

; Oliveira Jr., Osvaldo N.

; Camargo, Vanessa R.

; Maia, Lauro J. Q.

; Araújo, Cid B.

Journal of the Brazilian Chemical Society

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Jan 2017, Volume 28 Nº 1 Páginas 58 - 67

Resumo: En | Texto: En | PDF: En

We report on the synthesis of single-crystalline tellurium nanowires with different aspect-ratios prepared via facile surfactant-assisted synthesis under mild conditions. Short and long Te nanowires were synthesized by the reduction of tellurium dioxide by hypophosphorous acid with the assistance of polyoxyethylene (23) laurylether and cetyltrimethylammonium bromide, respectively. We obtained uniform single-crystal Te nanowires with diameter below 30 nm and tunable length from 600 nm to 5 µm. Short and long Te nanowires showed remarkable nonlinear absorption and their third-order nonlinear optical properties were investigated by the Z-scan technique with single 80 ps laser pulses at 532 nm. For the first time, it was observed a distinguished switching behavior from saturated absorption to reverse saturated absorption as a function of the laser intensity in one-dimensional Te nanostructures. Although both short and long Te nanowires display such interesting behavior, the short ones exhibited superior optical limiting performance.

https://doi.org/10.5935/0103-5053.20160145 1720 downloads

12.

Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil
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SILVA, GABRIELA L. DA

; LUFT, CAROLINA

; LUNARDELLI, ADROALDO

; AMARAL, ROBSON H.

; MELO, DENIZAR A. DA SILVA

; DONADIO, MÁRCIO V.F.

; NUNES, FERNANDA B.

; AZAMBUJA, MARCOS S. DE

; SANTANA, JOÃO C.

; MORAES, CRISTINA M.B.

; MELLO, RICARDO O.

; CASSEL, EDUARDO

; PEREIRA, MARCOS AURÉLIO DE ALMEIDA

; OLIVEIRA, JARBAS R. DE

Anais da Academia Brasileira de Ciências

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Ago 2015, Volume 87 Nº 2 Páginas 1397 - 1408

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Diversos estudos têm investigado as propriedades antinociceptivas, imunomoduladoras e anti-inflamatórias dos compostos encontrados no óleo essencial de lavanda (OEL), contudo, até o momento, ainda faltam dados mais substanciais. O objetivo deste estudo foi avaliar os efeitos antioxidantes, anti-inflamatórios e antinociceptivos do óleo essencial de lavanda. Para avaliação da atividade antioxidante foi realizado o ensaio de descoloração do radical 1,1-difenil-2-picrilhidrazil. A atividade anti-inflamatória foi testada utilizando dois modelos de inflamação aguda: indução de pleurisia por carragenina e edema de orelha induzido pela aplicação de óleo de cróton. A atividade antinociceptiva foi avaliada utilizando o modelo de dor provocada por formalina. OEL apresenta atividade antioxidante, sendo esta resposta dose-dependente. A resposta inflamatória provocada pela carragenina e pelo óleo de cróton foi reduzida com o pré-tratamento dos animais com OEL. No modelo de pleurisia, a droga usada como controle positivo, dexametasona, foi mais eficaz. Entretanto, no edema de orelha, o efeito antiedematogênico do óleo foi similar ao observado na dexametasona. No teste da formalina, o OEL inibiu consistentemente a nocicepção espontânea e apresentou efeito similar ao do tramadol. Os resultados deste estudo revelam a atividade (invivo) analgésica e anti-inflamatória do OEL, demonstrando seu importante potencial terapêutico.

Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The inflammatory response evoked by carrageenan and by croton oil was reduced through the pre-treatment of animals with LEO. In the pleurisy model, the drug used as positive control, dexamethasone, was more efficacious. However, in the ear swelling, the antiedematogenic effect of the oil was similar to that observed for dexamethasone. In the formalin test, LEO consistently inhibited spontaneous nociception and presented a similar effect to that of tramadol. The results of this study reveal (in vivo) the analgesic and anti-inflammatory activities of LEO and demonstrates its important therapeutic potential.

https://doi.org/10.1590/0001-3765201520150056 13665 downloads

13.

II Diretriz de Ressonância Magnética e Tomografia Computadorizada Cardiovascular da Sociedade Brasileira de Cardiologia e do Colégio Brasileiro de Radiologia
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Sara, Leonardo

; Szarf, Gilberto

; Tachibana, Adriano

; Shiozaki, Afonso Akio

; Villa, Alexandre Volney

; Oliveira, Amarino Carvalho de

; Albuquerque, Andrei Skromov de

; Rochitte, Carlos Eduardo

; Nomura, César Higa

; Azevedo, Clerio Francisco

; Jasinowodolinski, Dany

; Tassi, Eduardo Marinho

; Medeiros, Fabio de Morais

; Kay, Fernando Uliana

; Junqueira, Flávia Pegado

; Azevedo, Guilherme S. A.

; Monte, Guilherme Urpia

; Pinto, Ibraim Masciarelli Francisco

; Gottlieb, Ilan

; Andrade, Joalbo

; Lima, João A. C.

; Parga Filho, José Rodrigues

; Kelendjian, Juliana

; Fernandes, Juliano Lara

; Iquizli, Leonardo

; Correia, Luis C. L.

; Quaglia, Luiz Augusto

; Gonçalves, Luiz Flavio Galvão

; Ávila, Luiz Francisco

; Zapparoli, Marcello

; Hadlich, Marcelo

; Nacif, Marcelo Souto

; Barbosa, Márcia de Melo

; Minami, Márcio Hiroshi

; Bittencourt, Marcio Sommer

; Siqueira, Maria Helena Albernaz

; Silva, Marly Conceição

; Lopes, Marly Maria Uellendahl

; Marques, Mateus Diniz

; Vieira, Mônica La Rocca

; Coellho Filho, Otávio Rizzi

; Schvartzman, Paulo R.

; Santos, Raul D.

; Cury, Ricardo C.

; Loureiro, Ricardo

; Cury, Roberto Caldeira

; Sasdelli Neto, Roberto

; Macedo, Robson

; Cerci, Rodrigo Julio

; Faria Filho, Rui Alberto de

; Cardoso, Sávio

; Naves, Thiago

; Magalhães, Tiago Augusto

; Senra, Tiago

; Burgos, Ursula Maria Moreira Costa

; Moreira, Valéria de Melo

; Ishikawa, Walther Yoshiharu

Arquivos Brasileiros de Cardiologia

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Dez 2014, Volume 103 Nº 6 Páginas 1 - 86

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.5935/abc.2014S006 67586 downloads

14.

The pressor effect of angiotensin-(1-7) in the rat rostral ventrolateral medulla involves multiple peripheral mechanisms
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Oliveira, Rita C.

; Campagnole-Santos, Maria J.

; Santos, Robson A. S.

Clinics

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2013, Volume 68 Nº 2 Páginas 245 - 252

Resumo: En | Texto: En | PDF: En

OBJECTIVE: In the present study, the peripheral mechanism that mediates the pressor effect of angiotensin-(1-7) in the rostral ventrolateral medulla was investigated. METHOD: Angiotensin-(1-7) (25 pmol) was bilaterally microinjected in the rostral ventrolateral medulla near the ventral surface in urethane-anesthetized male Wistar rats that were untreated or treated (intravenously) with effective doses of selective autonomic receptor antagonists (atenolol, prazosin, methyl-atropine, and hexamethonium) or a vasopressin V1 receptor antagonist [d(CH2)5 -Tyr(Me)-AVP] given alone or in combination. RESULTS: Unexpectedly, the pressor response produced by angiotensin-(1-7) (16 ± 2 mmHg, n = 12), which was not associated with significant changes in heart rate, was not significantly altered by peripheral treatment with prazosin, the vasopressin V1 receptor antagonist, hexamethonium or methyl-atropine. Similar results were obtained in experiments that tested the association of prazosin and atenolol; methyl-atropine and the vasopressin V1 antagonist or methyl-atropine and prazosin. Peripheral treatment with the combination of prazosin, atenolol and the vasopressin V1 antagonist abolished the pressor effect of glutamate; however, this treatment produced only a small decrease in the pressor effect of angiotensin-(1-7) at the rostral ventrolateral medulla. The combination of hexamethonium with the vasopressin V1 receptor antagonist or the combination of prazosin, atenolol, the vasopressin V1 receptor antagonist and methyl-atropine was effective in blocking the effect of angiotensin-(1-7) at the rostral ventrolateral medulla. CONCLUSION: These results indicate that angiotensin-(1-7) triggers a complex pressor response at the rostral ventrolateral medulla that involves an increase in sympathetic tonus, release of vasopressin and possibly the inhibition of a vasodilatory mechanism.

https://doi.org/10.6061/CLINICS/2013(02)OA20 1546 downloads

15.

Homogeneidade de séries climatológicas em Minas Gerais
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Santos, Roziane S. dos

; Sediyama, Gilberto C.

; Oliveira, Robson A.

; Abrahão, Gabriel M.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Dez 2012, Volume 16 Nº 12 Páginas 1338 - 1345

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Os dados climáticos são de extrema importância nas diversas atividades humanas, por fornecerem muitas informações relativas ao meio ambiente e aos impactos nele decorrentes. Portanto, há necessidade de informações meteorológicas estatisticamente homogêneas visto que uma série temporal não homogênea pode comprometer a análise e a interpretação desses dados. Assim, este trabalho teve como objetivo estudar metodologias para avaliar a homogeneidade de séries de dados de temperaturas máximas e mínimas no Estado de Minas Gerais. A análise de homogeneidade foi feita por meio de três testes de identificação de pontos de descontinuidade nas séries temporais: Teste de Homogeneidade Normal Padrão (SNHT), teste de Pettitt e teste Buishand. Os testes apresentaram resultados muito similares sendo que a maior parte das séries mensais de temperatura foi considerada homogênea. As heterogeneidades ocorreram na década de 1990, principalmente em torno de 1997.

Climatic data are extremely important in many human activities; they provide much information about the environment and its impacts. So there is a need for weather data information, statistically homogeneous, because heterogeneity can compromise any time series analysis and interpretations of such data. Thus, this work aimed to study methodologies for assessing the homogeneity of data sets of maximum and minimum temperatures in the state of Minas Gerais, Brazil. The analysis of homogeneity was carried out by means of three tests for the identification of points of discontinuity in the series: Standard Normal Homogeneity Test (SNHT), Pettitt test and the Buishand test. The results of the tests were very similar, with most of the monthly series of temperature were considered homogeneous. Heterogeneities occurred in the 1990s, especially around 1997.

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