Objetivo: Caracterizar clínica paraclínica y epidemiológicamente el Lupus Eritematoso Sistèmico en los pacientes del Hospital Universitario Hernando Moncaleano Perdomo de Neiva Diseño: Descriptivo de corte transversal. Población: Pacientes atendidos en el Hospital Universitario de Neiva Hernando Moncaleano Perdomo con diagnóstico de lupus eritematoso sistèmico. Método: Recolección de datos a través de revisión documental de historias clínicas del Hospital Universitario de Enero 2009 a Enero 2014. Resultados: De un total de 104 pacientes con lupus eritematoso sistèmico encontrados en el periodo de estudio, la relación mujer: hombre fue de 5:1. Las comorbilidades de mayor frecuencia en el género femenino fueron la hipertensión arterial con un 26,7%, la fibromialgia con 12,7% y el síndrome de antifosfolípidos con 12, 7%; en el género masculino la hipertensión arterial, la fibromialgia y la falla cardiaca se presentaron cada una en un 27,7%. El 61,5% de las pacientes femeninas tuvieron manifestaciones en piel, el 51,1% refirieron artralgias y solo un 25,5% presentaron compromiso articular caracterizado por derrame sinovial. En los hombres las manifestaciones en piel estuvieron presentes en un 50%, las artralgias y el compromiso articular caracterizado por derrame sinovial fueron un 50% y 22,2% respectivamente. En los exámenes paraclínicos entre el 12,7% y el 31,4% de los pacientes tenían un lupus activo, determinado por la presencia de leucopenia, proteinuria y C3-C4 consumidos. En la muestra de pacientes femeninas las alteraciones paraclínicas más frecuentes se caracterizaron por hemoglobina <12 g/dl en 53,4% y trombocitopenia en 26,7%. Por su parte, 33,3% de los hombres presentaron trombocitopenia y 6,3% hemoglobina <12 g/dl.

Objective: To characterize clinically, paraclinical, and epidemiologically Systemic Lupus Erythematosus in patients at Hospital Universitario in Neiva Design: Descriptive cross sectional. Place: Hospital Universitario Hernando Moncaleano Perdomo, Neiva-Colombia. Population: Patients at Hospital Universitario Hernando Moncaleano Perdomo with diagnosis of Systemic Lupus Erythematosus. Method: Data collection of medical histories was performed. Results: Of 104 medical histories, the disease occurs five times more often in women than in men (ratio of 5:1). The most frequent comorbidities in females were hypertension with 26.74%, fibromyalgia and anti-phospholipid syndrome both with 12, 79%; In male patients hypertension, fibromyalgia and heart failure were presented each in a 27.78%. 61.53% of female patients had skin manifestations, 51.16% reported arthralgia and only 25.58% had joint involvement characterized by synovial effusion; in men skin manifestations were 50%, 50% arthralgia and articular involvement characterized by synovial effusion was 22.22%. In the laboratory test between 12.79% and 31.40% of patients had active lupus, determined by the presence of leukopenia, proteinuria and C3 and C4 consumed. In the sample of female patients the most frequent paraclinical alterations were characterized by hemoglobin <12 g / dl in 53.48% and 26, 74% of patients present thrombocytopenia. Meanwhile, 33.33% of men had thrombocytopenia and 6.38% had hemoglobin <12 g / dl.

The effect of benznidazole (BZL) on the expression and activity of P-glycoprotein (P-gp, ABCB1) and multidrug resistance-associated protein 2 (MRP2, ABCC2), the two major transporters of endogenous and exogenous compounds, was evaluated in differentiated THP-1 cells. BZL induced P-gp and MRP2 proteins in a concentration-dependent manner. The increase in mRNA levels of both transporters suggests transcriptional regulation. P-gp and MRP2 activities correlated with increased protein levels. BZL intracellular accumulation was significantly lower in BZL-pre-treated cells than in control cells. PSC833 (a P-gp inhibitor) increased the intracellular BZL concentration in both pre-treated and control cells, confirming P-gp participation in BZL efflux.

Introducción: el reflujo vésico-ureteral, presente en alrededor de un tercio de los niños con infección urinaria, es una enfermedad genéticamente determinada. Según la bibliografía, los hermanos de los niños con reflujo vésico-ureteral, tendrían entre 27-46% de probabilidades de ser portadores. Objetivo: valorar la incidencia de reflujo vésico-ureteral en hermanos de niños con reflujo, incluidos en una investigación sobre infección urinaria. Material y método: se estudió en forma, prospectiva y descriptiva, a todos los hermanos de niños con reflujo vésico-ureteral incluidos en el protocolo de estudio de infección urinaria entre el 18 de agosto de 1999 al 18 de agosto de 2000. Se solicitó consentimiento informado. Se estudió a todos los hermanos con examen de orina, urocultivo y ecografía renal. A los menores de 3 años y/o con antecedentes de infección urinaria o ecografía renal patológica, también se les realizó cistouretrografía miccional. Resultados: se incluyeron 9 niños con reflujo vésico-ureteral (casos índices), que contaban con 17 hermanos. Se obtuvo autorización para el estudio de todos (n=17). El rango de edades fue entre 3 meses y 16 años. Se encontraron 1/17 ecografías anormales: divertículo vesical, 2/8 (11,76%) cistouretrografía miccional patológicas: dos niños de 9 meses y 2 años, con reflujo vésico-ureteral grado II unilateral. Conclusiones: se destaca la excelente respuesta de los padres a estudiar niños supuestamente sanos en el ámbito hospitalario. Se encontró un porcentaje inferior al de la bibliografía (11,76%) de reflujo vésico-ureteral en hermanos sanos, posiblemente atribuible a la pequeña muestra y a diferencias metodológicas. Se destaca la importancia de diagnosticar reflujo vésico-ureteral en niños, previo a episodios infecciosos.

Introdução: o refluxo vésico-ureteral presente em aproximadamente 1/3 das crianças com infecção urinária, é uma doença genéticamente determinada. Conforme a bibliografia, os irmãos das crianças com refluxo vésico-ureteral, teriam entre 27-46% de probabilidade de serem portadores Objetivo: valorar a incidência de refluxo vésico-ureteral en irmãos de crianças com refluxos, incluídas em uma investigação sobre infecção urinária. Material e método: estudou-se em forma prospectiva e descriptiva, a todos os irmãos das crianças com refluxo vésico-ureteral incluídos no protocolo de estudo de infecção urinária entre 18-08-1999 e 18-08-2000 solicitou-se consentimento informado. Estudou-se todos os irmãos com: exame de urina, urocultivo e ecografia renal. As crianças com menos de três anos e/o com antecedentes de infecção urinária ou ecografia renal patológica, também realizou-se cistouretrografia miccional. Resultados: incluíram-se 9 crianças com refluxo vésico-ureteral (casos índices) que contavam com 17 irmãos. Obteve-se autorização para o estudo de todos (n=17). A idade foi entre 3 meses e 16 anos. Constatou-se: 1/17 ecografia patológica, divertículo vesical, 2/8 (11,76%) cistouretrografia miccional. patológicas: 2 crianças de 9 meses e 2 anos, com refluxo vésico-ureteral grau II unilateral Conclusões: destaca-se a excelente resposta dos pais a estudar crianças supostamente sadias no ámbito hospitalar. Percebeu-se uma percentagem inferior ao da bibliografia (11,76%) de refluxo vésico-ureteral em irmãos sadios, isso foi atribuído à pequena amostra, e a diferenças metodológicas. Destaca-se a importância de diagnosticar refluxo vésico-ureteral em crianças, prévio a episódios infecciosos.

Introduction: vesico-ureteral reflux (VUR) is found in near 1/3 of children with urinary tract infection (UTI). It is a genetically inherited illness. Bibliography says that siblings of children with VUR, have between 27-46% of risk of having it themselves. Objective: to know the incidence of VUR in siblings of children with it, included in a UTI research. Material and method: a prospective and descriptive study was done. There were included all of the siblings of children with VUR, enclosed in the UTI research, carried out between 18/08/1999 and 18/08/2000 in Pediatric Clinic B (CHPR). Inform consent was required. Each sibling was studied with: urine analysis, bacteriurie and renal ultrasound. Retrograde cistouretrography was also done to children under 3 years, and to those with previous UTI or pathologic findings at the ultrasound. Results: 9 children with VUR were included (index cases) and 17 siblings. Every authorization was obtained (n = 17). Ages were between 3 months and 16 years. 1/17 ultrasounds were pathologic: vesical diverticulous, 2/8 (11,76%) cystographies were pathologic: 2 girls (9 months and 2 years), with G II vesico-ureteral reflux. Conclusions: it is to point up, the excellent acceptance of parents to study healthy children at hospital field. The amount of children with VUR founded (11,76%) was lower to that observed by other researchers. Probably this depends on sample size and methodological differences. It is to emphasize the importance of founding VUR in children before UTI episodes.