SUMMARY OBJECTIVE: The objective of this study was to estimate the seroprevalence of severe acute respiratory syndrome coronavirus 2 antibodies in patients with tuberculosis. METHODS: This cross-sectional study was conducted at an outpatient tuberculosis clinic in Alvorada, RS, Brazil, with data collection between October and December 2020. Outpatients aged>18 years with active pulmonary tuberculosis, no prior history of coronavirus disease 2019, and no suspected coronavirus disease 2019 were included in the study. Whole blood samples were collected to perform the severe acute respiratory syndrome coronavirus 2 antibodies test. RESULTS: During the study period, 52 patients met the inclusion and were included in the analysis. Severe acute respiratory syndrome coronavirus 2 antibodies were positive in 16 (30.8%) patients. Male sex was more frequent among patients with negative severe acute respiratory syndrome coronavirus 2 antibodies than in patients with positive severe acute respiratory syndrome coronavirus 2 antibodies (86.1 vs. 56.3%, p=0.031). Contact with coronavirus disease 2019 case was more common in patients with positive severe acute respiratory syndrome coronavirus 2 antibodies compared with patients with negative severe acute respiratory syndrome coronavirus 2 antibodies (87.5 vs. 8.3%, p<0.0001). In a multivariate analysis, in a model including the variables such as male sex and contact with coronavirus disease 2019 case, only contact with coronavirus disease 2019 was independently associated with positive severe acute respiratory syndrome coronavirus 2 antibodies (OR 77.0, 95%CI 11.5–512.4, p<0.0001). CONCLUSION: This study revealed a seroprevalence of 30.8% severe acute respiratory syndrome coronavirus 2 among patients with tuberculosis.

ABSTRACT Purpose: After partial hepatectomy (PH), the remaining liver (RL) undergoes regenerative response proportional to the host. Limited literature exists on hepatic viability after tissue injury during hypothermic preservation. Spectroscopy measures cellular fluorescence and is explored for tissue characterization and parameter investigation. This study aimed to assess fluorescence analysis (spectroscopy) in evaluating liver viability and its relationship with hepatic tissue regeneration 24 hours after PH. Additionally, we analyzed liver regeneration in RL after 70% partial hepatectomy under hypothermic conditions with laser irradiation. Methods: Fifty-six Wistar rats were divided into four groups: total non-perfused liver (control), total perfused liver, partial hepatectomy “in situ”, and partial hepatectomy “ex situ”. Tissue analysis was performed at 0 and 24 hours using spectroscopy with laser devices emitting at 532 (green) and 405 nm (violet). Results: Spectroscopy identified tissue viability based on consistent results with Ki67 staining. The fluorescence spectra and Ki67 analysis displayed similar patterns, linking proliferative activity and absorption intensity. Conclusions: Fluorescence spectroscopy proves to be promising for real-time analysis of cellular activity and viability. Metabolic activity was observed in groups of live animals and hypothermically preserved samples, indicating cellular function even under blood deprivation and hypothermic conditions.

ABSTRACT Breast cancer (BC) is the most common neoplasm in women globally. Healthy eating, a characteristic of the Mediterranean diet (MD), has been associated with a lower risk of developing BC, although this relationship remains inconclusive. This study aimed to analyse the relationship between MD adherence and the risk of BC development. A case-control study was carried out with 181 women divided into two groups: with BC (n= 90) and without breast cancer (WBC) (n= 91). Clinical and reproductive aspects were investigated through interviews, and food consumption was assessed through a food frequency questionnaire (FFQ). Adherence to the MD was evaluated using the MD score. Statistical analysis was performed using SPSS® software, with p<0.05. The results showed that the main type of breast carcinoma was invasive ductal carcinoma, positive for oestrogen or progesterone receptors. The mean MD score was 4.08 ± 1.61 and 4.22 ± 1.69 among women with BC and without BC, respectively, with no difference between the groups. The risk assessment of BC with respect to adherence to MD showed no significant difference after adjustments were made in the logistic regression models. In conclusion, this study showed that women with and without breast cancer had “medium adherence” to MD, with no difference between the groups. In the group of women investigated, adherence to MD did not affect the risk of developing the disease.

RESUMEN El cáncer de mama (CM) es la neoplasia más frecuente entre las mujeres del mundo, y la alimentación saludable, característica del patrón de la dieta mediterránea (DM), se ha asociado con un menor riesgo de desarrollar CM, aunque esta relación sigue sin ser concluyente. El objetivo del estudio fue analizar la relación entre la adherencia a la DM y el riesgo para el desarrollo de CM. Estudio de casos y controles realizados con 181 mujeres divididas en dos grupos: con cáncer de mama (n=90) y sin cáncer de mama (SCM; n= 91). Los aspectos clínicos y reproductivos se evaluaron a través de una entrevista; consumo de alimentos a través del cuestionario de frecuencia alimentaria (FFQ) y adherencia a la dieta mediterránea evaluada según la puntuación de DM. El análisis estadístico se realizó mediante el software SPSS®, considerando valores significativos de p<0,05. Los resultados mostraron que el principal tipo de carcinoma de mama fue el carcinoma ductal invasivo, positivo para el receptor de estrógenos y progesterona. La puntuación media de DM fue de 4,08 ± 1,61 entre las mujeres con CM y de 4,22 ± 1,69 entre las que no tenían la enfermedad, sin diferencia entre los grupos. La evaluación del riesgo de CM en relación con la adherencia a la DM mostró que, después de ajustes en los modelos de regresión logística, no hubo diferencia significativa. En conclusión, el estudio muestra que las mujeres con y sin CM tuvieron “adherencia media” a la DM, sin diferencia entre los grupos. También mostró que, en el grupo de mujeres investigadas, la adherencia a la DM no influyó en el riesgo para el desarrollo de la enfermedad.

RESUMO Contexto: O tratamento convencional da doença inflamatória intestinal (DII) é baseado na terapia medicamentosa, mas diferentes estudos têm mostrado aumento progressivo do uso de medicina complementar e alternativa (MCA) na abordagem dos pacientes. As modalidades de MCA mais usadas compreendem: acupuntura, medicina tradicional chinesa, medicina ayurvédica, homeopatia e fitoterapia, bem como práticas mais modernas, como aromaterapia e reflexologia. Os dados do uso de MCA no Brasil são escassos e não há estudos entre pacientes brasileiros com DII. Objetivo: O objetivo do estudo foi avaliar a frequência e os fatores associados com o uso de MCA entre pacientes com DII, além de estimar a satisfação com o uso de MCA. Métodos: Foi realizado estudo transversal em pacientes adultos ambulatoriais com DII oriundos de dois centros de referência no sudeste do Brasil, com amostra de 227 indivíduos. Foi aplicado questionário semiestruturado contendo produtos como - chá, probióticos, ômega 3 ou glutamina, homeopatia e fitoterapia, além de fatores associados ao uso de MCA e a satisfação do paciente. Utilizamos estatística descritiva, testes de associação (P<0,05) e regressão logística para análise estatística. Resultados: No total, 126 pacientes com doença de Crohn e 101 com retocolite ulcerativa foram incluídos. A média de idade foi 41,19±14,49 anos e 57,27% eram do sexo feminino. O tempo desde o diagnóstico foi de 10,58±7,5 anos, e a maioria dos pacientes estava em remissão clínica. Vinte e nove pacientes (12,8%) relataram ter usado MCA para o tratamento de DII, como chá (5,29%), probióticos (5,29%), ômega-3 ou glutamina (1,76%), homeopatia (0,88%) e fitoterápicos (0,44%). Apesar da baixa frequência, os pacientes ficaram satisfeitos com o uso (>50%). Não houve diferença entre o uso de MCA entre os pacientes com doença de Crohn em comparação com pacientes com retocolite ulcerativa (P=0,1171). Os fatores associados com o uso de MCA foram qualidade de vida regular ou ruim (Odds ratio 2,084; intervalo de confiança de 95% 1,147-3,786, P=0,0159) e menor tempo desde o diagnóstico (Odds ratio 0,956; intervalo de confiança de 95% 0,918-0,995; P=0,0260). Conclusão: A prevalência do uso de MCA foi baixa, mas satisfatória entre os pacientes com DII. O uso de MCA tem sido associada a baixa qualidade de vida e menor duração da doença.

ABSTRACT Background: The conventional treatment of inflammatory bowel disease (IBD) is based on drug therapy, but different studies have shown a progressive increase in the use of complementary and alternative medicine (CAM). The most used CAM comprises of acupuncture, traditional Chinese medicine, Ayurvedic medicine, homeopathy, and herbal medicine, as well as more modern practices, including aromatherapy and reflexology. Data from CAM use in Brazil has previously been scarce and there are no studies among Brazilian patients with IBD. Objective: The aim of the study was to evaluate the frequency of, and factors associated with the use of CAM among IBD patients in Brazil, in addition to estimating the satisfaction with CAM use. Methods: A cross-sectional study was performed in adult IBD outpatients from two Southeastern Brazilian referral centers, with a total a sample of 227 individuals. A semi-structured questionnaire was used containing CAM products - tea, probiotics, omega 3 or glutamine, homeopathy, and herbal therapy, and factors associated with CAM use and patient satisfaction. We used descriptive statistics, association tests (P<0.05) and logistic regression for statistical analyses. Results: In total, 126 patients with Crohn’s disease and 101 with ulcerative colitis were included. The mean age was 41.19±14.49 years and 57.27% were female. The time since diagnosis was 10.58±7.5 years, and most patients were in clinical remission. Twenty-nine patients (12.8%) reported having used CAM for IBD treatment, such as tea (5.29%), probiotics (5.29%), omega-3 or glutamine (1.76%), homeopathy (0.88%), and herbal therapies (0.44%). Despite the low frequency, patients were satisfied (>50%). There was no difference between CAM use in Crohn’s disease as compared to ulcerative colitis patients (P=0.1171). The factors associated with the use of CAM were regular or poor quality of life (odds ratio 2.084; 95% confidence interval 1.147-3.786, P=0.0159) and a shorter time since diagnosis (odds ratio 0.956; 95% confidence interval 0.918-0.995; P=0.0260). Conclusion: The prevalence of CAM use was low, but satisfactory among Brazilian IBD patients. The application of CAM has been associated with poor quality of life and shorter disease duration compared to patients with no use of CAM.

The physiological quality of the seeds is an aspect of great relevance for the establishment of a culture. However, plant management conditions during seed production can influence its quality. Given the above, the objective was to evaluate the physiological quality of crambe seeds from plants submitted to different water regimes. The experimental design used was in randomized blocks, composed of five water conditions in the soil, based on field capacity (100, 85, 70, 55 and 40%) that consisted of treatments, with four replications. After harvesting, the seeds were sent to the seed laboratory, processed and then evaluated for water content, germination and vigor. Crambe plants grown in soil water conditions between 59 to 61% of the field capacity produce seeds of better physiological quality, demonstrated by the performance in the germination and vigor tests.

A qualidade fisiológica das sementes é um aspecto de grande relevância para o estabelecimento de uma cultura. No entanto, as condições de manejo das plantas durante a produção de sementes podem influenciar na sua qualidade. Diante do exposto, objetivou-se avaliar a qualidade fisiológica de sementes de crambe oriundas de plantas submetidas a diferentes regimes hídricos. O delineamento experimental utilizado foi em blocos casualizados, composto por cinco condições de água no solo, baseadas na capacidade de campo (100, 85, 70, 55 e 40%) que consistiram nos tratamentos, com quatro repetições. Após a colheita, as sementes foram encaminhadas ao laboratório de sementes, beneficiadas e em seguida, avaliadas quanto ao teor de água, germinação e vigor. Plantas de crambe cultivadas em condições de água no solo entre 59 a 61% da capacidade de campo produzem sementes de melhor qualidade fisiológica, demonstrado pelo desempenho nos testes de germinação e vigor.

RESUMO Objetivo: compreender as percepções dos valores do aleitamento materno para lactantes custodiadas pelo sistema penal. Método: pesquisa qualitativa e fenomenológica, baseada nos valores schelerianos, com realização de sete entrevistas fenomenológicas com lactantes privadas de liberdade, na unidade prisional feminina do Rio de Janeiro, Brasil. As entrevistas foram realizadas entre novembro 2020 e maio de 2021. Após transcrições das falas, sucedeu-se à análise de conteúdo para o tratamento dos resultados. Resultados: identificaram-se duas categorias para o valor vital: o valor do vínculo como processo de expansão; a lactante como valor de segurança e proteção para o bebê. Por meio da percepção das lactantes privadas de liberdade, o valor vital identificado atua como proteção ao aleitamento materno dentro do espaço prisional, fortalecendo a saúde da mulher e criança. Conclusão: ao desvelar o valor vital, observa-se que este está relacionado à vida cotidiana da mãe e do bebê dentro do cárcere. Entretanto, a instituição prisional não satisfaz as reais necessidades vitais das lactantes custodiadas, sendo, então, necessário redirecionar a prática da amamentação no espaço prisional, transformando-o em ambiente legítimo, que valorize os sentidos do ato de amamentar.

ABSTRACT Objective: to understand the perceptions about the values of breastfeeding for nursing mothers under custody of the penitentiary system. Method: a qualitative and phenomenological research study based on the Schelerian values, with seven phenomenological interviews conducted with nursing mothers deprived of their freedom in a women's prison unit from Rio de Janeiro, Brazil. The interviews took place between November 2020 and May 2021. Content analysis for treatment of the results was performed after transcribing the testimonies. Results: two categories were identified for the vital value: The value of bonding as a breastfeeding expansion process; and The nursing mother as a safety and protection value for the infant. Through the perception of the nursing mothers deprived of their freedom, the vital value identified acts as protection for breastfeeding within the prison setting, strengthening the woman's and child's health. Conclusion: by unveiling the vital value, it is observed that it is related to the mother's and infant's everyday life in prison. However, the prison institution does not meet the real vital needs of imprisoned nursing mothers, with the consequent need to redirect the breastfeeding practice in the prison setting, transforming it into a legitimate environment that values the meaning of breastfeeding.

RESUMEN Objetivo: comprender las percepciones con respecto a los valores de la lactancia materna para madres lactantes que se encuentran bajo la custodia del sistema penitenciario. Método: investigación cualitativa y fenomenológica basada en los valores schelerianos, en la que se realizaron siete entrevistas fenomenológicas con madres lactantes privadas de su libertad en la unidad penitenciaria para mujeres de Río de Janeiro, Brasil. Las entrevistas tuvieron lugar entre noviembre de 2020 y mayo de 2021. Después de transcribir los testimonios, se realizó un análisis de contenido para procesar los resultados. Resultados: se identificaron dos categorías para el valor vital, a saber: El valor del vínculo como proceso de expansión de la lactancia materna; y La madre lactante como valor de seguridad y protección para el recién nacido. A través de la percepción de las madres lactantes privadas de su libertad, el valor vital identificado actúa como protección de la lactancia materna dentro del espacio penitenciario, fortaleciendo así tanto la salud de la mujer como la del recién nacido. Conclusión: al revelar el valor vital, se observa que está relacionado con la vida cotidiana de la madre y del recién nacido dentro de la prisión. Sin embargo, la institución penitenciaria no satisface las verdaderas necesidades vitales de las madres lactantes que tiene bajo su custodia; en consecuencia, es necesario redireccionar la práctica de la lactancia materna en el espacio penitenciario, transformándolo en un ambiente legítimo que valorice los significados de la lactancia materna.

Resumo Os objetivos deste artigo são identificar e mapear as ações de promoção, proteção e apoio à amamentação que são realizadas dentro do sistema prisional. Revisão de escopo cujas buscas foram realizadas nas bases de dados Literatura Latino-Americana e do Caribe em Ciências da Saúde, Biblioteca Virtual em Saúde, Medical Literature, Analysis, and Retrieval System Online, via PubMed, Scientific Electronic Library Online e no Portal de Periódicos da Capes, entre outros. Obtiveram-se 465 estudos, destes, removeram-se 177 duplicatas e 175 por exclusão de título, 56 foram eliminados pelo resumo e 8 não foram encontrados. Após seleção e leitura pelo time de revisão, retiraram-se 16 artigos, constituindo-se então 10 artigos com resultados relevantes para promoção à amamentação no espaço prisional, 13 com resultados importantes para proteção à amamentação no espaço prisional e 10 com potenciais achados para apoio à amamentação no espaço prisional. É necessário fomentar a amamentação no cárcere com ações de promoção, proteção e apoio, pois muitas crianças ainda são impedidas de ser amamentadas logo após o nascimento, o que caracteriza desigualdade em relação à população em liberdade.

Abstract This article aims to identify and map the actions to promote, protect and support breastfeeding that are carried out within the prison system. Scope review, whose searches were performed in the Latin American and Caribbean Health Sciences Literature and Nursing databases, Virtual Health Library, Medical Literature, Analysis, and Retrieval System Online, via PubMed, Scientific Electronic Library Online and the Capes Periodicals Portal. A total of 465 studies were obtained, 177 duplicates and 175 by title exclusion were removed, 56 articles were eliminated by abstract, and eight articles were not found. After selection and reading by the review team, 16 articles were removed, thus constituting ten articles with relevant results for breastfeeding promotion in prison, 13 articles with important results for breastfeeding protection in prison, and ten articles with potential findings for breastfeeding support in prison. It is necessary to promote breastfeeding in prison with promotion, protection and support actions, because many children are still prevented from being breastfed soon after birth, which characterizes inequality in relation to the population in freedom.

Resumo Os objetivos deste artigo são identificar e mapear as ações de promoção, proteção e apoio à amamentação que são realizadas dentro do sistema prisional. Revisão de escopo cujas buscas foram realizadas nas bases de dados Literatura Latino-Americana e do Caribe em Ciências da Saúde, Biblioteca Virtual em Saúde, Medical Literature, Analysis, and Retrieval System Online, via PubMed, Scientific Electronic Library Online e no Portal de Periódicos da Capes, entre outros. Obtiveram-se 465 estudos, destes, removeram-se 177 duplicatas e 175 por exclusão de título, 56 foram eliminados pelo resumo e 8 não foram encontrados. Após seleção e leitura pelo time de revisão, retiraram-se 16 artigos, constituindo-se então 10 artigos com resultados relevantes para promoção à amamentação no espaço prisional, 13 com resultados importantes para proteção à amamentação no espaço prisional e 10 com potenciais achados para apoio à amamentação no espaço prisional. É necessário fomentar a amamentação no cárcere com ações de promoção, proteção e apoio, pois muitas crianças ainda são impedidas de ser amamentadas logo após o nascimento, o que caracteriza desigualdade em relação à população em liberdade.

Abstract This article aims to identify and map the actions to promote, protect and support breastfeeding that are carried out within the prison system. Scope review, whose searches were performed in the Latin American and Caribbean Health Sciences Literature and Nursing databases, Virtual Health Library, Medical Literature, Analysis, and Retrieval System Online, via PubMed, Scientific Electronic Library Online and the Capes Periodicals Portal. A total of 465 studies were obtained, 177 duplicates and 175 by title exclusion were removed, 56 articles were eliminated by abstract, and eight articles were not found. After selection and reading by the review team, 16 articles were removed, thus constituting ten articles with relevant results for breastfeeding promotion in prison, 13 articles with important results for breastfeeding protection in prison, and ten articles with potential findings for breastfeeding support in prison. It is necessary to promote breastfeeding in prison with promotion, protection and support actions, because many children are still prevented from being breastfed soon after birth, which characterizes inequality in relation to the population in freedom.

Resumo Objetivo Validar o conteúdo do diagnóstico de enfermagem processos familiares interrompidos em mulheres privadas de liberdade. Métodos Estudo metodológico de validação de conteúdo do diagnóstico de enfermagem processos familiares interrompidos, realizado com 24 especialistas, no período de agosto a dezembro de 2019. Os especialistas foram enfermeiros, psicólogos e assistentes sociais com experiência clínica e teórica na área do estudo. O recrutamento destes ocorreu a partir da plataforma Lattes, do universo relacional do pesquisador e técnica de amostragem “snow ball”. Os dados foram analisados pelo programa estatístico R, sendo calculado o Índice de Validade de Conteúdo com base no modelo da diversidade preditiva. Também foram calculados os Intervalos de Confiança de 95% e foi aplicado o teste de Wilcoxon, tendo como valor de referência para a hipótese nula o Índice de Validade de Conteúdo ≥ 0,9. Resultados Foram identificados 12 elementos do diagnóstico em estudo, os quais apresentaram significância estatística, com exceção do item “ausência da figura paterna” o qual foi excluído da proposta diagnóstica. O item “depressão” também foi excluído, conforme sugestão dos especialistas, apesar da significância estatística. Conclusão O conteúdo do diagnóstico de enfermagem processos familiares interrompidos em mulheres privadas de liberdade foi considerado válido pelos especialistas e pode nortear a prática clínica do enfermeiro, sobretudo na operacionalização do Processo de Enfermagem.

Abstract Objective To validate the Interrupted Family Processes nursing diagnosis content in women deprived of liberty. Methods This is a methodological study to validate the Interrupted Family Processes nursing diagnosis content, carried out with 24 experts, from August to December 2019. Experts were nurses, psychologists and social workers with clinical and theoretical experience in the area of study. These were recruited from the Plataforma Lattes, the researcher’s relational universe and snowball sampling technique. Data were analyzed using the R statistical program, and Content Validity Index was calculated based on the predictive diversity model. The 95% Confidence Intervals were also calculated and the Wilcoxon test was applied, having Content Validity Index ≥ 0.9 as a reference value for the null hypothesis. Results Twelve elements of the diagnosis under study were identified, which were statistically significant, with the exception of Father Absence item, which was excluded from the diagnostic proposal. Depression item was also excluded, as suggested by the experts, despite the statistical significance. Conclusion The Interrupted Family Processes nursing diagnosis content in women deprived of liberty was considered valid by the experts and can guide the clinical practice of nurses, especially in the operationalization of the Nursing Process.

Resumen Objetivo Validar el contenido del diagnóstico de enfermería Interrupción de los procesos familiares en mujeres privadas de la libertad. Métodos Estudio metodológico de validación de contenido del diagnóstico de enfermería Interrupción de los procesos familiares, realizado con 24 especialistas, en el período de agosto a diciembre de 2019. Los especialistas fueron enfermeros, psicólogos y asistentes sociales con experiencia clínica y teórica en el campo de estudio. Este reclutamiento se realizó a partir de la plataforma Lattes, del universo relacional del investigador y técnica de muestreo “snow ball”. Los datos fueron analizados por el programa estadístico R y el Índice de Validez de Contenido fue calculado con base en el modelo de la diversidad predictiva. También se calcularon los Intervalos de confianza de 95 % y se aplicó la prueba de Wilcoxon, en la que el valor de referencia para la hipótesis fue el Índice de Validez de Contenido ≥ 0,9. Resultados Se identificaron 12 elementos del diagnóstico estudiado que presentaron significación estadística, excepto el ítem “ausencia de la figura paterna” que fue excluido de la propuesta diagnóstica. El ítem “depresión” también fue excluido, de acuerdo con lo sugerido por los especialistas, a pesar de su significación estadística. Conclusión El contenido del diagnóstico de enfermería Interrupción de los procesos familiares en mujeres privadas de la libertad fue considerado válido por los especialistas y puede nortear la práctica clínica del enfermero, sobre todo en la operacionalización del Proceso de Enfermería.

Abstract Pockmarks are circular or elliptical structures formed at the seabed by the expulsion of gas from the subsurface. They are widely distributed along the continental margin off southeastern Brazil and can be over a kilometer wide and 100 meters deep. However, studies concerning the organic characteristics of these pockmark areas are scarce. This study sought to evaluate the organic composition of the sedimentary matter in pockmark areas located in the continental slope region of the southern Brazilian coast. Hydrocarbons, sterols, long-chain alcohols, stable isotopes of C and N, total organic carbon, and total nitrogen were assessed to provide an organic molecular characterization of the pockmarks located in the Southwestern Atlantic Ocean. These compounds did not reflect the organic characteristics of the scape of fluids that generate pockmark structures.

RESUMO A qualidade fisiológica das sementes é um aspecto de grande relevância para o estabelecimento de uma cultura. No entanto, as condições de manejo das plantas durante a produção de sementes podem influenciar na sua qualidade. Diante do exposto, objetivou-se avaliar a qualidade fisiológica de sementes de crambe oriundas de plantas submetidas a diferentes regimes hídricos. O delineamento experimental utilizado foi em blocos casualizados, composto por cinco condições de água no solo, baseadas na capacidade de campo (100, 85, 70, 55 e 40%) que consistiram nos tratamentos, com quatro repetições. Após a colheita, as sementes foram encaminhadas ao laboratório de sementes, beneficiadas e em seguida, avaliadas quanto ao teor de água, germinação e vigor. Plantas de crambe cultivadas em condições de água no solo entre 59 a 61% da capacidade de campo produzem sementes de melhor qualidade fisiológica, demonstrado pelo desempenho nos testes de germinação e vigor.

ABSTRACT The physiological quality of the seeds is an aspect of great relevance for the establishment of a culture. However, plant management conditions during seed production can influence its quality. Given the above, the objective was to evaluate the physiological quality of crambe seeds from plants submitted to different water regimes. The experimental design used was in randomized blocks, composed of five water conditions in the soil, based on field capacity (100, 85, 70, 55 and 40%) that consisted of treatments, with four replications. After harvesting, the seeds were sent to the seed laboratory, processed and then evaluated for water content, germination and vigor. Crambe plants grown in soil water conditions between 59 to 61% of the field capacity produce seeds of better physiological quality, demonstrated by the performance in the germination and vigor tests.

Abstract Background The relationship between Multidrug Resistant-Gram Negative Bacteria (MDR-GNB) infection and colonization in critically ill COVID-19 patients has been observed, however, it is still poorly understood. This study evaluated the risk factors for acquiring MDR-GNB in patients with severe COVID-19 in Intensive Care Units (ICU). Methods This is a nested case-control study in a cohort of 400 adult patients (≥ 18 years old) with COVID-19, hospitalized in the ICU of 4 hospitals in the city of Curitiba, Brazil. Cases were critical COVID-19 patients with one or more MDR GNB from any surveillance and/or clinical cultures were taken during their ICU stay. Controls were patients from the same units with negative cultures for MDR-GNB. Bivariate and multivariate analyses were done. Results Sixty-seven cases and 143 controls were included. Independent risk factors for MDR bacteria were: male gender (OR = 2.6; 95% CI 1.28‒5.33; p = 0.008); the hospital of admission (OR = 3.24; 95% CI 1.39‒7.57; p = 0.006); mechanical ventilation (OR = 25.7; 95% CI 7.26‒91; p < 0.0001); and desaturation on admission (OR = 2.6; 95% CI 1.27‒5.74; p = 0.009). Conclusions Male gender, desaturation, mechanical ventilation, and the hospital of admission were the independent factors associated with MDR-GNB in patients in the ICU with COVID-19. The only modifiable factor was the hospital of admission, where a newly opened hospital posed a higher risk. Therefore, coordinated actions toward a better quality of care for critically ill COVID-19 patients are essential.

Resumo Fundamento: A doença de Chagas leva à redução da capacidade funcional. Entretanto, o estágio em que o comprometimento funcional é detectável permanece obscuro. Objetivos: O presente estudo teve como objetivo comparar a capacidade funcional de pacientes em diferentes estágios da doença de Chagas e de indivíduos saudáveis e verificar os determinantes do consumo de oxigênio de pico (VO2pico). Métodos: Em um estudo transversal, foram selecionados 160 indivíduos, 35 saudáveis e 125 com doença de Chagas. No grupo chagásico, 61 (49%) estavam na forma indeterminada da doença, 45 (36%) com cardiomiopatia chagásica (CC) e função cardíaca preservada e 19 (15%) com disfunção cardíaca e CC dilatada. Os dados foram analisados por meio de análise de regressão univariada e multivariada. A significância estatística foi fixada em 5%. Resultados: Pacientes na forma indeterminada da doença apresentaram capacidade funcional semelhante a indivíduos saudáveis (p> 0,05). Pacientes com ChC e função cardíaca preservada apresentaram VO2pico menor que os pacientes na forma indeterminada (p <0,05), mas apresentaram valores de VO2pico semelhantes ao ChC dilatado (p = 0,46). A idade, sexo masculino, classe funcional da NYHA, pressão arterial diastólica, razão entre a velocidade do fluxo transmitral diastólico precoce e a velocidade anular mitral diastólica precoce, a fração de ejeção do ventrículo esquerdo (FEVE) e o diâmetro diastólico final do ventrículo esquerdo foram associados à capacidade funcional. Porém, apenas idade, sexo masculino, FEVE e classe funcional da NYHA permaneceram associados ao VO2pico no modelo final (R2 ajustado = 0,60). Conclusão: Pacientes com CC apresentam menor capacidade funcional do que pacientes na forma indeterminada. FEVE, idade, sexo masculino e classe funcional da NYHA foram determinantes do VO2pico em pacientes com doença de Chagas.

Abstract Background: Chagas disease leads to reduced functional capacity. However, the stage at which functional impairment is detectable remains unclear. Objectives: The present study was addressed to compare the functional capacity of patients at different stages of Chagas disease and healthy individuals and to verify the determinants of peak oxygen uptake (VO2peak). Methods: In a cross-sectional study, 160 individuals were selected, 35 healthy and 125 with Chagas disease. In the Chagasic group, 61 (49%) were in the indeterminate form of the disease, 45 (36%) with Chagas cardiomyopathy (ChC) and preserved cardiac function and 19 (15%) with cardiac dysfunction and dilated ChC. The data were analyzed using univariate and multivariate regression analysis. Statistical significance was set at 5%. Results: Patients in the indeterminate form of disease showed similar functional capacity to healthy individuals (p>0.05). Patients with ChC and preserved cardiac function had lower VO2peak than patients in the indeterminate form (p<0.05), but showed similar VO2peak values than dilated ChC (p=0.46). The age, male sex, NYHA functional class, diastolic blood pressure, ratio of the early diastolic transmitral flow velocity to early diastolic mitral annular velocity, left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter were associated with functional capacity. However, only age, male sex, LVEF and NYHA functional class, remained associated with VO2peak in the final model (adjusted R2=0.60). Conclusion: Patients with ChC had lower functional capacity than patients in the indeterminate form. LVEF, age, male sex and NYHA functional class were determinants with VO2peak in patients with Chagas disease.

ABSTRACT Species of Stemphylium genus are responsible for many plant diseases, as solanaceas in which stemphylium leaf blight, which productivity of these crops can be negatively affected due lacking in available fungicides. Therefore, the purpose of this work was to determine a culture medium that would allow of Stemphylium sp. mycelial growth isolated from onion for a later evaluation of melaleuca oil (M. alternifolia) effect under different in vitro concentrations. For this, in the first test eight culture media were tested: Potato-Dextrose-Agar (PDA); Juice V-8® 5% Agar; V-8® Juice 10% Agar; Juice V-8® Agar 20%; Tomato Juice 5% (ST5%); Tomato Juice 10% (ST10%); Tomato Juice 20% (ST20%) and Malt Extract Agar 2%. At ten days and fifteen days, the highest growth was identified with treatments ST20% and PDA and, conversely, treatments V8-5% and V8-10% the lowest growth. In the second trial, melaleuca oil was tested in five concentrations (0; 0, 2; 0,4; 0,6 and 0,8%) diluted in PDA and the MGR (mycelial growth rate) was determined. The melaleuca essential oil reduced the Stemphylium sp. mycelial growth with better fit to the quadratic equation y=28,074x2 - 46,136x + 19,94. The MGR was reduced by the oil added to culture medium and could represent a promising alternative for this pathogen control.

RESUMO Espécies do gênero Stemphylium são responsáveis por uma série de doenças de plantas, como as solanáceas, com destaque para a queima-de-estenfílio, que afeta a produtividade de culturas carentes em fungicidas disponíveis. Diante disso, propôs-se com esse trabalho a determinação de um meio de cultura que viabilizasse o crescimento micelial de Stemphylium sp. isolado de cebola para posterior avaliação do efeito do óleo de melaleuca (M. alternifolia), sob diferentes concentrações in vitro, no desenvolvimento desse patógeno. Para isso, no primeiro ensaio foram testados oito meios de cultura: Batata-Dextrose-Ágar (BDA); Suco V-8® Ágar a 5%; Suco V-8® Ágar a 10%; Suco V-8® Ágar a 20%; Suco de Tomate a 5% (ST5%); Suco de Tomate a 10% (ST10%); Suco de Tomate a 20% (ST20%) e Extrato de Malte Ágar 2%. Aos 10 dias e aos 15 dias o maior crescimento foi identificado com os tratamentos ST20% e BDA e opostamente, os tratamentos V8-5% e V8-10% o menor crescimento. No segundo ensaio, o óleo de melaleuca foi testado em cinco concentrações (0; 0,2; 0,4; 0,6 e 0,8%) diluído em BDA e se determinou o IVCM (índice de velocidade do crescimento micelial). O óleo essencial de melaleuca reduziu significativamente o crescimento micelial de Stemphylium sp. com melhor ajuste à equação quadrática y = 28,074x2 - 46,136x + 19,94. Conclui-se que o meio de cultura ST20% proporcionou o maior crescimento in vitro e que o óleo de melaleuca adicionado ao meio de cultura nas concentrações de 0,6 e 0,8% foram capazes de reduzir o IVCM, podendo representar alternativa promissora para o controle deste patógeno.

RESUMO Objetivo: Investigar a relação da antropometria e composição corporal com o equilíbrio postural dinâmico em idosas com baixa Densidade Mineral Óssea (DMO). Métodos: 45 idosas (≥ 60 anos), baixa DMO e diagnóstico nutricional entre baixo peso e sobrepeso. Para a avaliação da composição corporal utilizou-se a densitometria por emissão de raios x de dupla energia e exame antropométrico para aferir: massa corporal (kg), estatura (cm) e índice de massa corporal (IMC) (k/m2). A avaliação do equilíbrio postural dinâmico foi realizada pelo teste clínico mini Balance Master Evaluation System, pelo teste computadorizado Balance Master ® System e pelos testes Sit-to-Stand e Step Up/Over. Resultados: Houve correlação negativa do miniBESTest (r = − 0,566; p ≤ 0,001) e tempo de subir e descer um degrau (r = − 0,393; p ≤ 0,007) com a massa gorda, e correlação positiva do miniBESTest (r = 0,526; p ≤ 0,001) e tempo de subir e descer um degrau com a massa muscular (r = 0,297; p ≤ 0,04). Quanto às variáveis antropométricas, apenas a estatura apresentou correlação positiva (r = 0,296; p ≤ 0,04) com a velocidade no teste de sentar-se e levantar-se. Conclusão: A massa magra reduz as oscilações posturais. Em contrapartida, a massa gorda interfere de forma negativa no equilíbrio postural dinâmico de mulheres com baixa DMO. A estatura esteve relacionada ao equilíbrio postural dinâmico: quanto mais altas as idosas pior era seu equilíbrio. Nível de Evidência II, Estudos prognósticos - Investigação do efeito de característica de um paciente sobre o desfecho da doença.

ABSTRACT Objective: To investigate the relationship between anthropometry and body composition with dynamic postural balance in elderly women with low bone mineral density (BMD). Methods: 45 older women (≥ 60 years), low BMD and nutritional diagnosis of low weight to overweight. For the assessment of body composition, Dual energy X-ray emission densitometry and anthropometric examination were used to measure: body mass (kg), height (cm) and BMI (k/m2). The assessment of dynamic postural balance was performed by the mini Balance Master Evaluation System clinical test and the computerized Balance Master® System test by the Sit to Stand and Step Up/Over tests. Results: There was a negative correlation between miniBESTest (r = − 0.566; p ≤ 0.001) and time to ascend and descend step (r = − 0.393; p ≤ 0.007) with fat mass, and positive correlation with miniBESTest (r = 0.526; p ≤0.001) and time to go up and down a step with muscle mass (r = 0.297; p ≤ 0.04). As for anthropometric variables, only height showed a positive correlation (r = 0.296; p ≤ 0.04) with the speed in the sit and stand test. Conclusion: Lean mass reduces postural oscillations; in contrast, fat mass negatively interfered with dynamic postural balance in women with low BMD. Height was related to dynamic postural balance, the taller the elderly, the worse their balance. Level of Evidence II, Prognostic Studies - Investigating the Effect of a Patient Characteristic on the Outcome of Disease.