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ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral pH was 0.2 mg/mL. Energy Dispersive X-ray analysis confirmed elemental composition of the purified bioflocculant in mass proportion (%w/w): carbon (21.41), oxygen (35.59), sulphur (26.16), nitrogen (0.62) and potassium (7.48). Fourier Transform Infrared Spectroscopy (FTIR) indicated the presence of hydroxyl, carboxyl, methoxyl and amino group in the bioflocculant. The bioflocculant produced by S. platensis removed chemical oxygen demand (COD) in river water and meat processing wastewater at efficiencies of 63.1 and 46.6% respectively and reduced their turbidity by 84.3 and 75.6% respectively. The high flocculating rate and removal efficiencies displayed by S. platensis suggests its industrial application in wastewater treatment.

ABSTRACT Objective Evaluate the propensity of cardiovascular risk in rural workers and, through the Framingham Risk Score (FRS), relate this risk with the classification of Body Mass Index (BMI). Subjects and methods This study is characterized as descriptive and exploratory, with the participation of 138 subjects, ranging between 25-73 years old. Clinical and laboratory analysis of the risk factors contained in the FRS were performed, in addition to the determination of BMI, blood pressure, smoking and physical inactivity. Results The procedures indicated a low risk of a coronary event in 10 years with 70.3% of the population. In contrast, 88.4% of the subjects were overweight. It was evidenced a risk improvement as the BMI increased, since 96.4% of high-risk cases were overweight or obese. Conclusion Results suggest larger prevalence of intermediary or high FRS for women with higher BMI, which was not observed in men.

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