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Abstract The incorporation of alternative materials in partial replacement of clinker is studied to reduce the CO2 emissions that occur in the production of Portland cement. The influence of using mesquite ash as an alternative filler in LC3 cement pastes was analyzed. The alkali content of the system was adjusted by washing the ash, thus reducing the potassium content. The filler was replaced at 0, 25%, 50%, 75%, and 100% washed ash, and the same for unwashed ash. Subsulfation was corrected by adding an extra gypsum percentage. Paste performance was assessed by rheology and compressive strength, supported by hydration kinetics and microstructural development. Mesquite ash is a potential alternative filler for application in LC3. Washed ash makes the system purer, contributing to hydration and mechanical performance, however, natural ash also brings promising results.

Resumo A incorporação de materiais alternativos em substituição parcial ao clínquer é estudada afim de diminuir a emissão de CO2 que ocorre na produção do cimento Portland. Analisou-se a influência do uso da cinza de algaroba como fíler alternativo em pastas de cimento LC3. O teor de álcali do sistema foi ajustado lavando a cinza e, assim, reduzindo o teor de potássio. O fíler foi substituído em 0, 25%, 50%, 75% e 100% de cinza lavada e o mesmo para cinza não lavada. A subsulfatação foi corrigida adicionando-se uma porcentagem de gesso extra. O desempenho da pasta foi avaliado pela reologia e resistência à compressão, sustentada pela cinética de hidratação e desenvolvimento microestrutural. A cinza de algaroba é um potencial fíler alternativo para aplicação em LC3. A cinza lavada torna o sistema mais puro, contribuindo na hidratação e desempenho mecânico, entretanto, a cinza natural também traz resultados promissores.

https://doi.org/10.1590/0366-69132023693913452

Filtrates from cultures of endophytic fungi isolated from leaves of Copaifera oblongifolia (Fabaceae) affect germination and seedling development differently
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Silva, P. S.

; Royo, V. A.

; Valerio, H. M.

; Fernandes, E. G.

; Queiroz, M. V.

; Fagundes, M.

Brazilian Journal of Biology

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2023, Volume 83 elocation e242070

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo As interações entre fungos endofíticos (FEs) e suas plantas hospedeiras variam de positivas, neutras a negativas. Os resultados destas interações podem variar dependendo do órgão da planta hospedeira infectada. FEs isolados de folhas de algumas espécies de plantas têm potencial para serem usados como agentes inibidores da germinação de sementes e no controle de plantas invasoras. Os objetivos deste estudo foram identificar os FEs presentes nas folhas de Copaifera oblongifolia e avaliar o papel destes fungos na germinação das sementes e no desenvolvimento das plântulas. Um total de 11 espécies de FEs foi isolado das folhas de C. oblongifolia e identificado através da sequência dos espaçadores internos transcritos do DNA ribossomal nuclear. As espécies de FEs isoladas são generalistas e provavelmente devem ser transmitidas horizontalmente. Os resultados dos testes de germinação mostraram que filtrados destes isolados fúngicos podem afetar diferentemente a germinação das sementes e o desenvolvimento das plântulas de C. oblongifolia. As espécies Curvularia intermedia, Neofusicoccum parvum, Pseudofusicoccum stromaticum e Phomopsis sp. afetaram negativamente a germinação das sementes de C. oblongifolia. Dentre estas espécies devemos destacar que N. parvum reduziu a germinação e a sobrevivência das plântulas em 89 e 222%, respectivamente. Além disso, Cochiliobolus intermedius afetou negativamente o desenvolvimento das plântulas. Assim, o uso combinado de N. parvum e C. intermedius, ou de produtos do metabolismo destas espécies de fungos, têm potencial para serem usados no manejo de plantas invasoras.

Abstract Interactions between endophytic fungi (EFs) and their host plants range from positive to neutral to negative. The results of such interactions can vary depending on the organ of the infected host plant. EFs isolated from the leaves of some species of plants have potential for use as agents to inhibit seed germination and control invasive plants. The objectives of this study were to identify EFs present in the leaves of Copaifera oblongifolia and to evaluate the role of these fungi in seed germination and seedling development. A total of 11 species of EFs were isolated, which were identified using the internal transcribed spacers (ITS) sequence of the nuclear ribosomal DNA. The isolated species of EFs are generalists and probably are transmitted horizontally. Laboratory tests revealed that filtrates of these fungal isolates differently affect seed germination and seedling development of C. oblongifolia. The species Curvularia intermedia, Neofusicoccum parvum, Pseudofusicoccum stromaticum and Phomopsis sp. negatively affected seed germination, with N. parvum standing out for its negative effects, inhibiting seedling germination and survival in 89 and 222%, respectively. In addition, Cochliobolus intermedius negatively affected seedling development. Thus, the combined use of N. parvum and C. intermedius, or products from the metabolism of these microorganisms, in the control of invasive plants deserves attention from future studies.

https://doi.org/10.1590/1519-6984.242070 422 downloads

Emotional, hyperactivity and inattention problems in adolescents with immunocompromising chronic diseases during the COVID-19 pandemic
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Campos, Reinan T.

; Lindoso, Livia

; Sousa, Renan A. de

; Helito, Alberto C.

; Ihara, Bianca P.

; Strabelli, Claudia A.A.

; Paradelas, Levi M.V.

; Carneiro, Beatriz O.L.

; Cardoso, Maria Paula R.

; Souza, Jean Paulo V. de

; Freire, Marianna R. de M.

; Astley, Camilla

; Laurentino, Moisés F.

; Buscatti, Izabel M.

; Kozu, Katia

; Aikawa, Nadia E.

; Sallum, Adriana M.E.

; Ferreira, Juliana CO.

; Simon, Juliana R.

; Viana, Vivianne S.L.

; Queiroz, Ligia B.

; Gualano, Bruno

; Roschel, Hamilton

; Pereira, Rosa Maria R.

; Toma, Ricardo K.

; Watanabe, Andréia

; Grangeiro, Patricia M.

; Casella, Caio B.

; Farhat, Sylvia C.

; Polanczyk, Guilherme V.

; Campos, Lucia Maria A.

; Silva, Clovis A.

Clinics

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2023, Volume 78 elocation 100167

Resumo: En | Texto: En | PDF: En

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics.

https://doi.org/10.1016/j.clinsp.2023.100167

VIBRATION TRANSMISSIBILITY OF THE COFFEE FRUIT-PEDUNCLE SYSTEM: A FORCED VIBRATION STUDY OF HIGH FREQUENCY AIMING MECHANICAL HARVESTING
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Villibor, Geice P.

; Santos, Fábio L.

; Queiroz, Daniel M.

; Khoury Junior, Joseph K

; Pinto, Francisco A. C.

Engenharia Agrícola

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2023, Volume 43 Nº 1 elocation e20220201

Resumo: En | Texto: En | PDF: En

ABSTRACT Semi-mechanized and mechanized harvesting use machines that promote the transference of vibrational energy and impact to achieve the detachment of coffee fruits. The aim of this study was to evaluate the vibration transmissibility in coffee fruit-peduncle systems, using high-speed cameras, submitted to high frequency harmonic excitation in different combinations between frequency and amplitude of vibration, identifying working ranges suitable to perform selective harvesting. Vibration transmissibility was determined for the coffee fruit-peduncle systems, for the maturation stages unripe and ripe that were subjected to a sinusoidal harmonic displacement, in which the input parameters were frequency (35, 45 and 55 Hz) and peak-to-peak amplitude (3.5, 5.0 and 6.5 mm). An experiment was used to study the effect of frequency and amplitude on vibration transmissibility in a completely randomized design in a factorial scheme 3 x 3 x 2, with three replications. The frequency of 35 Hz, associated with the amplitudes 3.5-6.5 mm, was the one that most influenced the results of vibration transmissibility. For the frequency of 55 Hz and amplitude of 6.5 mm, in the ripe maturation stage, the vibration transmissibility was higher than 1.0, which could be a suitable combination for selective coffee harvesting.

https://doi.org/10.1590/1809-4430-eng.agric.v43n1e20210201/2023

Parasitological diagnosis in ornamental freshwater fish from different fish farmers of five Brazilian states
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Dominguez, H. N.

; Balian, S. C.

; Relvas, R. S.

; Soares, H. S.

; Queiroz, M. R.

; Martins, M. L.

; Cardoso, P. H. M.

Brazilian Journal of Biology

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2023, Volume 83 elocation e270067

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract This study aimed to search parasites in 333 ornamental fish from five Brazilian states (Ceará, Minas Gerais, São Paulo, Paraná and Santa Catarina). Fish were sent from eight farms located in the municipalities of Fortaleza, Patrocínio do Muriaé, São Francisco do Glória, Cascavel, Timbó, Iguape, Jacareí and Mairinque. All fish received anesthesia earlier to euthanasia procedures. After the search for parasites, it was verified that 70.6% (235/333) of fishes were infected by at least one type of parasite, being 12 types of parasites identified: monogeneans, digenean metacercariae, cestodes, nematodes, Lernaea cyprinacea, trichodinids, Piscinoodinium pillulare, Ichthyophthirius multifiliis, diplomonad flagellates, Ichthyobodo sp., Chilodonella sp., and Tetrahymena sp. The proportion of infected fishes among the farms is compared through statistical tests, besides, animal handling adopted in each farm is also discussed. The importance of ensuring fish health in order to make the ornamental freshwater fish industry economically viable and reduce losses in production is highlighted.

Resumo O objetivo deste estudo foi pesquisar parasitas presentes em 333 peixes ornamentais de cinco estados brasileiros (Ceará, Minas Gerais, São Paulo, Paraná e Santa Catarina). Os peixes eram oriundos de oito pisciculturas localizadas nos municípios de Fortaleza, Patrocínio do Muriaé, São Francisco do Glória, Cascavel, Timbó, Iguape, Jacareí e Mairinque. Todos os peixes foram anestesiados e eutanasiados para a pesquisa de parasitas. Após análise microscópica, foi verificado que 70.6% (235/333) dos peixes estavam infectados por ao menos uma espécie de parasita, sendo identificados 12 tipos: monogenoides, metacercárias, cestoides, nematoides, Lernaea cyprinacea, tricodinídeos, Piscinoodinium pillulare, Ichthyophthirius multifiliis, flagelados diplomonados, Ichthyobodo sp., Chilodonella sp., e Tetrahymena sp. A proporção de peixes infectados entre cada piscicultura é comparada através de testes estatísticos, além disso, o manejo adotado pelas diferentes pisciculturas também é discutido. A importância de assegurar a sanidade de peixes ornamentais, de forma a tornar a atividade economicamente viável e reduzir perdas na produção é enfatizada.

https://doi.org/10.1590/1519-6984.270067

Study of Surface Modification of Niobium Caused by Nitriding and Cathodic Cage Deposition
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Macedo, Francisco R. C.

; Monção, Renan M.

; Queiroz, José C. A.

; Libório, Maxwell S.

; Costa, José A. P.

; Rossino, Luciana S.

; Danelon, Miguel R.

; Feitor, Michelle C.

; Costa, Thércio H. C.

; Sousa, Rômulo R. M.

Materials Research

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2023, Volume 26 elocation e20220613

Resumo: En | Texto: En | PDF: En

In this work, plasma nitriding (PN) and cathode cage plasma deposition (CCPN) treatments were carried out with temperatures of 400 and 450 °C to evaluate the modifications caused on the surface of pure niobium samples. XRD, SEM, Vickers microhardness, and sphere-disk analyses were used to characterize the coatings’ composition, morphology, hardness, and wear resistance. The results showed that the treated samples increased hardness and wear resistance, with the sample submitted to CCPN at 450 °C presenting the best tribological behavior.

https://doi.org/10.1590/1980-5373-mr-2022-0613

Film Deposition by Duplex Treatment with Hastelloy Cage on AISI 6160 Steel
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Medeiros Filho, Mário V.

; Silva, Lucas P. da

; Libório, Maxwell S.

; Queiroz, José C. A.

; Coan, Karine S.

; Rossino, Luciana S.

; Costa, Thércio H. C.

; Sousa, Rômulo R. M.

Materials Research

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2023, Volume 26 elocation e20220625

Resumo: En | Texto: En | PDF: En

AISI 6160 steel is used to manufacture cutting blades and springs due to its high tensile strength and good ductility. However, it has low mechanical strength and low wear resistance. In this work, a duplex treatment consisting of CCPD followed by PN was performed to improve the surface properties of the steel. In addition, XRD, optical microscopy, microhardness, and sphere-disk tribological analysis were used to verify the surface changes of the samples. The results showed a significant improvement in surface hardness and better wear resistance of AISI 6160 Steel submitted to Duplex treatment. The sample submitted to the CCPD treatment for 4 hours and, after nitriding at 500 °C for 2 hours, presented higher surface hardness and wear resistance than the sample only subjected to plasma deposition, characterizing the efficiency of the use of the duplex treatment adopted in the present work.

https://doi.org/10.1590/1980-5373-mr-2022-0625

Construção Automática de Múltiplas Árvores Circulatórias com Controle de Invasão de Território
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DE AQUINO, L. C. M.

; ROCHA, B. M.

; DE QUEIROZ, R. A. B.

Trends in Computational and Applied Mathematics

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2023, Volume 24 Nº 2 Páginas 293 - 303

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Este trabalho apresenta um algoritmo baseado no método Constrained Constructive Optimization (CCO) que possibilita gerar um modelo da estrutura geométrica de um sistema vascular com n árvores utilizando condições de contorno fisiológicas de pressão e fluxo, levando em conta a minimização do volume intravascular total durante a construção do modelo. Aplica-se o algoritmo proposto na geração de um modelo em um domínio bidimensional convexo. Os resultados mostram que o modelo criado reproduz características morfométricas encontradas em árvores vasculares reais. CCO (CCO fluxo Aplicase Aplica se convexo reais

ABSTRACT In this work we propose an algorithm based on the Constrained Constructive Optimization (CCO) method that generates a geometric model of the vascular system structure with n trees, considering fisiological conditions of flow, pressure, and taking into account the minimum intravascular volume while constructing the model. We use this algorithm to generate a geometric model of some vascular system on a convex two-dimensional perfusion domain. The results show that the model replicates morphometric features found in real vascular trees. CCO (CCO trees flow pressure twodimensional two dimensional domain

https://doi.org/10.5540/tcam.2023.024.02.00293

Synthesis of Eugenol-Fluorinated Triazole Derivatives and Evaluation of Their Fungicidal Activity
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Lima, Ângela M. A.

; Paula, Wenderson T. de

; Leite, Iris C. H. L.

; Gazolla, Poliana A. R.

; Abreu, Lucas M. de

; Fonseca, Victor R.

; Romão, Wanderson

; Lacerda Jr., Valdemar

; Queiroz, Vagner T. de

; Teixeira, Róbson R.

; Costa, Adilson V.

Journal of the Brazilian Chemical Society

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Out 2022, Volume 33 Nº 10 Páginas 1200 - 1210

Resumo: En | Texto: En | PDF: En

Eugenol (C10H12O2, 4-allyl-2-methoxyphenol) is a phenolic natural product that has several biological activities and possibilities of applications. It is herein described the synthesis of eugenol-fluorinated triazole derivatives and evaluation of their fungicidal activity. The reaction of eugenol with epichlorohydrin resulted in the preparation of (±)-2-((4-allyl-2-methoxyphenoxy)methyl)oxirane (1) in 88% yield. The azidolysis of 1 with sodium azide gave the azido-alcohol (±)-1-(4-allyl-2-methoxyphenoxy)-3-azidopropan-2-ol (2) in 94% yield. The CuAAc reaction between compound 2 and different terminal alkynes afforded a series of eleven derivatives (3a 3k) within 48-80% yield. All the synthesized compounds were characterized by infrared (IR) and nuclear magnetic resonance (1H and 13C) spectroscopies and high-resolution mass spectrometry. The in vitro inhibitory activity of the compounds on the mycelial growth of a strain of Colletotrichum sp., that causes anthracnose disease on papaya fruits, was evaluated. The best result was observed for compound 1-(4-allyl-2-methoxyphenoxy)-3-(4-(2-fluorophenyl)-1H-1,2,3-triazol-1-yl)propan-2-ol (3d) that showed a mean growth-inhibition zone of 5.10 mm in a well-diffusion assay, and may serve as a template for additional structural modifications, aiming for more potent fungicidal activity.

https://doi.org/10.21577/0103-5053.20220040

10.

Effect of two oral formulations of cannabidiol on responses to emotional stimuli in healthy human volunteers: pharmaceutical vehicle matters
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Crippa, José A.

; Pereira Junior, Luiz C.

; Pereira, Lívia C.

; Zimmermann, Patrícia M.

; Brum Junior, Liberato

; Rechia, Letícia M.

; Dias, Isabella

; Hallak, Jaime E.

; Campos, Alline C.

; Guimarães, Francisco S.

; Queiroz, Regina H.

; Zuardi, Antonio W.

Brazilian Journal of Psychiatry

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Fev 2022, Volume 44 Nº 1 Páginas 15 - 20

Resumo: En | Texto: En | PDF: En

Objective: To compare plasma concentrations of cannabidiol (CBD) following oral administration of two formulations of the drug (powder and dissolved in oil), and to evaluate the effects of these distinct formulations on responses to emotional stimuli in healthy human volunteers. Methods: In a randomized, double-blind, placebo-controlled, parallel-group design, 45 healthy male volunteers were randomly assigned to three groups of 15 subjects that received either 150 mg of CBD powder; 150 mg of CBD dissolved in corn oil; or placebo. Blood samples were collected at different times after administration, and a facial emotion recognition task was completed after 150 min. Results: There were no significant differences across groups in the subjective and physiological measures, nor in the facial emotion recognition task. However, groups that received the drug showed statistically significant differences in baseline measures of plasma CBD, with a significantly greater difference in favor of the oil formulation. Conclusion: When administered as a single 150-mg dose, neither formulation of oral CBD altered responses to emotional stimuli in healthy subjects. The oil-based CBD formulation resulted in more rapid achievement of peak plasma level, with an approximate fourfold increase in oral bioavailability.

https://doi.org/10.1590/1516-4446-2020-1684

11.

Mathematical modeling and thermodynamic properties in the drying of citron watermelon seeds
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Leite, Daniela D. de F.

; Queiroz, Alexandre J. de M.

; Figueirêdo, Rossana M. F. de

; Santos, Francislaine S. dos

; Silva, Semirames do N.

; Santos, Dyego da C.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Jan 2022, Volume 26 Nº 1 Páginas 67 - 74

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A melancia africana é um produto agrícola de excelente potencial econômico. Suas sementes são muito utilizadas para extração de óleo, servindo como fonte energética, demonstrando características nutricionais que a habilitam como produto a ser estudado. Assim, objetivou-se caracterizar quanto à composição físico-química, as sementes de melancia africana, além de determinar a cinética de secagem, ajustar modelos matemáticos aos dados, determinar os coeficientes de difusividade efetiva e propriedades termodinâmicas. As sementes foram submetidas à secagem em secador convectivo, variando-se a temperatura de secagem, com velocidade do ar de 1,0 m s-1. Com o aumento da temperatura de secagem diminuiu o teor de água, atividade de água, teor de cinzas, acidez total titulável, lipídios e açúcar redutor. As sementes de melancia africana são ricas em lipídios e cinzas, tem baixos teores de açúcares e baixa acidez; as cinéticas de secagem foram muito bem descritas pelos modelos Dois Termos e Aproximação da Difusão, seguidos pelos modelos de Midilli e Page, que resultaram em ajustes aceitáveis. A difusividade efetiva acompanhou o aumento da temperatura de secagem, e este comportamento foi satisfatoriamente ajustado por equação do tipo Arrhenius. As variações de entalpia e entropia foram reduzidas com a temperatura de secagem, com aumentos da energia livre de Gibbs.

ABSTRACT Citron watermelon is an agricultural product of excellent economic potential. Its seeds are widely used for oil extraction, serving as an energy source, showing nutritional characteristics that make them a suitable product to be studied. Thus, the objective was to characterize citron watermelon seeds regarding their physicochemical composition, in addition to determining drying kinetics, fitting mathematical models to the data, and determining the effective diffusivity coefficients and thermodynamic properties. The seeds were dried in a convective dryer, varying the drying temperature, with air velocity of 1.0 m s-1. With the increase in drying temperature, there were reductions in moisture content, water activity (aw), ash concentration, total titratable acidity, lipids and reducing sugar. Citron watermelon seeds are rich in lipids and ash, have low sugar concentration and low acidity; their drying kinetics was very well described by the Two Terms and Approximation of Diffusion models, followed by the models of Midilli and Page, which resulted in acceptable fits. Effective diffusivity accompanied the increase in drying temperature, and this behavior was well fitted by an Arrhenius-type equation. Enthalpy and entropy variations were reduced with drying temperature, with increments in Gibbs free energy.

https://doi.org/10.1590/1807-1929/agriambi.v26n1p67-74 76 downloads

12.

Antarctic organisms as a source of antimicrobial compounds: a patent review
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SILVA, MAURÍCIO B.

; FEITOSA, ALEXYA O.

; LIMA, IGOR G.O.

; BISPO, JAMES R.S.

; SANTOS, ANA CAROLINE M.

; MOREIRA, MAGNA S.A.

; CÂMARA, PAULO E.A.S.

; ROSA, LUIZ HENRIQUE

; OLIVEIRA, VALÉRIA M.

; DUARTE, ALYSSON W.F.

; QUEIROZ, ALINE C.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Supl. 1 elocation e20210840

Resumo: En | Texto: En | PDF: En

Abstract Currently, antimicrobial resistance has become a global public health problem, which has made the need for new antimicrobial compounds to deal with resistant infections an emergency. However, environments that once offered so many innovative molecules, now already exhaustively exploited, do not meet this need. In this context, a geographically isolated, under-explored and extreme environment, such as Antarctica, which holds organisms with unique physiological and biochemical characteristics, assumes great importance as a potential source of new compounds with antimicrobial activity. In this patent review, we investigate the state of technological development in the field of antimicrobial compounds obtained from Antarctic organisms, highlighting the main countries and researchers active in the field, the species utilized, the compounds obtained, and their possible therapeutic applications. As results, few patent documents were found, however they encompass a wide diversity of compounds and species, indicating a great antimicrobial potential present in Antarctic biota, including compounds active against the most important human pathogenic microorganisms, such as including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus spp. and multi-resistant Mycobacterium tuberculosis. Furthermore, due to the increasing trend in patent applications, a significant rise in the number of patents in this area is expected in the coming years.

https://doi.org/10.1590/0001-3765202220210840

13.

Extracellular hydrolytic enzymes produced by yeasts from Antarctic lichens
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DA SILVA, MAYANNE KARLA

; DA SILVA, AVERLANE V.

; FERNANDEZ, PAULA M.

; MONTONE, ROSALINDA C.

; ALVES, RODRIGO P.

; DE QUEIROZ, ALINE C.

; DE OLIVEIRA, VALÉRIA M.

; DOS SANTOS, VIVIANE P.

; PUTZKE, JAIR

; ROSA, LUIZ HENRIQUE

; DUARTE, ALYSSON W.F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Supl. 1 elocation e20210540

Resumo: En | Texto: En | PDF: En

Abstract In the Antarctic environment, yeasts are versatile eukaryotes that have shown wide dispersion in different substrates, producing active enzymes in extreme conditions, but their relevance in biotechnological applications is largely unknown. The aim of this study was to evaluate the production of extracellular hydrolases by yeasts isolated from Antarctic lichens and molecularly identify these isolates. From a total of 144 isolates on the screening, 109 (76%) produced at least one of the hydrolases tested, with most activities for proteases 59 (41%), cellulases 58 (40%), esterases 57 (39%), lipases 29 (20%), amylases 23 (16%) and pectinases 20 (14%). Among these isolates, 76 were identified, most belonged to the phylum Basidiomycota (n=73) with the dominance of Vishniacozyma victoriae (n=27), Cystobasidium alpinum (n=3), Mrakia niccombsii (n=3), Cystobasidium laryngis (n=2), Bannozyma yamatoana (n=2), Holtermanniella nyarrowii (n=2), and Glaciozyma martinii (n=2). This study is the first one reporting extracellular enzyme production by yeasts isolated from thallus of the species of Antarctic lichens Lecania brialmontii, Polycauliona candelaria, Usnea capillacea, Cladonia metacorallifera, and Polycauliona regalis. With these data, it’s possible to confirm lichens as a source of hydrolase-producing yeasts, reinforcing the potential of these microorganisms in bioprospecting studies of catalytic molecules from polar regions that may be useful in promising biotechnological applications.

https://doi.org/10.1590/0001-3765202220210540

14.

Antarctic environments as a source of bacterial and fungal therapeutic enzymes
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LIMA, IGOR G.O.

; BISPO, JAMES R.S.

; AGOSTINHO, ADSON Y.H.

; QUEIROZ, ALINE C. DE

; MOREIRA, MAGNA SUZANA A.

; PASSARINI, MICHEL RODRIGO Z.

; OLIVEIRA, VALÉRIA M. DE

; SETTE, LARA D.

; ROSA, LUIZ HENRIQUE

; DUARTE, ALYSSON WAGNER F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Supl. 1 elocation e20210452

Resumo: En | Texto: En | PDF: En

Abstract Microbial therapeutic enzymes are the protagonists in the pharmacological treatment of different human diseases. The intrinsic enzymatic characteristics, such as high affinity and specificity to the corresponding substrate, enable effective therapies, with minimal adverse effects and complete remission. However, immunogenicity, short half-life, low enzymatic yield, and low selectivity regarding available enzyme drugs are currently the main obstacles to their development and the broad adherence to therapeutic protocols. By harboring adapted and still unexplored microbial life, environments of extreme conditions, such as Antarctica, become especially important in the prospecting and development of new enzymatic compounds that present higher yields and the possibility of genetic improvement. Antarctic microorganisms have adaptation mechanisms, such as more fluid cell membranes, production of antifreeze proteins and enzymes with more malleable structures, more robust, stable, selective catalytic sites for their respective substrates, and high antioxidant capacity. In this context, this review aims to explore enzymes synthesized by bacteria and fungi from Antarctica as potential drug producers, capable of providing therapeutic efficacy, less adverse effects, and lower production costs with highlight to L-Asparaginase, collagenase, superoxide dismutase and ribonucleases. In addition, this review highlights the unique biotechnological profile of these Antarctic extremophile microorganisms.

https://doi.org/10.1590/0001-3765202220210452

15.

Development of a TD-NMR Method to Monitor Brazil Nuts Oil Content: A Green and Low-Cost Based Approach
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Ribeiro, Úrsula A.

; Queiroz, Leonardo C.

; Marassi, Agide G.

; Carvalho, André S.

; Barros, Gabriel A.

; Consalter, Daniel M.

; Bezerra, Jaqueline A.

; Santos, Alan D. C.

; Colnago, Luiz Alberto

; Machado, Marcos B.

Journal of the Brazilian Chemical Society

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Jul 2021, Volume 32 Nº 7 Páginas 1405 - 1412

Resumo: En | Texto: En | PDF: En

Growth in the international popularity of Brazil nuts oil (Bertholletia excelsa, Bonpl.) has demanded a closer look at quality aspects of the nuts. In this context, time-domain 1H nuclear magnetic resonance spectroscopy (TD-NMR) can be a useful tool for assessing the lipid portion of such matrices. In this work, a TD-NMR method was developed and validated to assess the oil content directly from Brazil nuts following the ISO 10565:1998 and DOQ-CGCRE-008. Full validation was addressed covering the working range from 0.67 to 25.00 g (coefficient of determination, R2= 0.9993). Limits of detection (LOD) and quantification (LOQ) were 0.20 and 0.67 g, respectively. The repeatability and intermediate precision assays met the guide requirements. Oil contents obtained in two laboratories (different equipment) were statistically similar (66.93 ± 4.69 and 70.13 ± 4.15%), highlighting the method’s robustness. The developed approach has the potential to be adopted as an industrial reference method for in situ monitoring of Brazil nuts oil contents.

https://doi.org/10.21577/0103-5053.20210039 105 downloads

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