Resumo Introdução A goiaba é um fruto amplamente utilizado como alimento e é considerada planta medicinal em países tropicais e subtropicais. Pesquisas têm mostrado que o fruto contém constituintes químicos com abrangente uso clínico. Além disso, a maior parte das substâncias utilizadas no tratamento contra câncer foi isolada a partir de produtos naturais. Objetivo Avaliar o potencial citotóxico, mutagênico, antimutagênico e quimioprotetor da fruta liofilizada de Psidium guajava, a goiaba, in vivo. Método A citotoxicidade, a mutagenicidade e a antimutagenicidade foram avaliadas em três diferentes dosagens (0,625, 1,25 e 2,50 g/kg) de goiaba. Resultados Os resultados mostraram que a goiaba não apresentou atividade citotóxica e mutagênica no ensaio de micronúcleo em sangue periférico e que não houve alterações nos valores de ALT e AST, indicando ausência de toxicidade hepática. Nos animais tratados com a goiaba, a dose de 0,625 mg/kg significativamente reduziu os danos induzidos pela doxorrubicina. Conclusão Esses resultados mostraram que o consumo de goiaba é seguro e capaz de proteger o material genético de alterações genômicas.

Abstract Background Guava is a fruit widely used as food and is considered a medicinal plant in the tropical and subtropical countries. Scientific research has shown that the fruit contains chemical constituents with comprehensive clinical use. In addition, most of the substances used in cancer treatment have been isolated from natural products. Objective To evaluate the cytotoxic, mutagenic, antimutagenic, and chemoprotective potential of the freeze-dried fruit of Psidium guajava, guava, in vivo. Method Cytotoxicity, mutagenicity, and antimutagenicity were evaluated in three different dosages (0.625, 1.25, 2.50 g/kg) of guava. Results The results show that guava does not present cytotoxic 2 and mutagenic activity in the micronucleus assay in peripheral blood and there were no alterations in ALT and AST values showing the absence of hepatic toxicity. In animals treated with guava, the dose of 0.625 mg/kg significantly reduced the damage induced by doxorubicin. Conclusion These results show that guava consumption is safe as it is also capable of protecting the genetic material from changes.

CONTEXTO: Los estudiantes de la enseñanza media experimentan en esta fase experiencias estresantes, como la elección de qué profesión seguir y los preparativos para los exámenes de admisión, lo que puede predisponer al desarrollo del Síndrome de Burnout. OBJETIVO: Este estudio tuvo como objetivo verificar la correlación entre el Síndrome de Burnout y el estilo de vida de los estudiantes de enseñanza media. MÉTODOS: Estudio transversal, cuantitativo, realizado con estudiantes de la enseñanza media. Para recoger los datos, se utilizaron el cuestionario Estilo de Vida Fantástico y el Maslach Burnout Inventory . Para estimar y probar la asociación entre las dimensiones de la Síndrome de Burnout y el estilo de vida entre los estudiantes, se empleó el coeficiente de correlación de Pearson. RESULTADOS: El estudio tuvo la participación de 966 estudiantes, con un promedio de edad de 16,61 años. Se observó que el 12,1% presentaba indicativos de Síndrome de Burnout. Se observó una relación negativa entre agotamiento emocional y sueño, cinturón de seguridad, estrés y sexo seguro (-0,225; p<0,001); incredulidad y el cigarrillo y las drogas (-0,206, p <0,001); y positiva para eficacia profesional, familia y amigos (0,224; p <0,001), sueño, cinturón de seguridad, estrés y sexo seguro (0,282; p <0,001) e introspección (0,245; p <0,001). CONCLUSIÓN: Se puede concluir que las prácticas saludables de vida adoptadas por los estudiantes de la enseñanza media pueden estar relacionadas con los bajos índices de estrés en esta fase.

INTRODUÇÃO: Os estudantes do ensino médio vivenciam nesta fase experiências estressantes, como a escolha de qual profissão seguir e os preparativos para os exames admissionais, o que pode predispor ao desenvolvimento da Síndrome de Burnout. OBJETIVO: Este estudo teve como objetivo analisar a relação entre a Síndrome de Burnout e o estilo de vida dos estudantes de ensino médio MÉTODOS: Estudo transversal, quantitativo, realizado com estudantes do ensino médio. Para a coleta de dados foram utilizados o questionario Estilo de Vida Fantastico e o Maslach Burnout Inventory - Student Survey (MBI-SS) . Para estimar e testar a associac'ao entre as dimensoes da Sindrome de Burnout e estilo de vida entre os estudantes empregou-se o coeficiente de correlac'ao de Pearson. RESULTADOS: Participaram do estudo 966 estudantes, com media de idade de 16,61 anos. Observou-se que 12,1% apresentaram indicativos de Sindrome de Burnout. Observou-se relac'ao negativa entre exaustao emocional e sono, cinto de seguranc'a, estresse e sexo seguro (-0,225; p<0,001). CONCLUSÃO: Pode-se concluir que práticas saudáveis de vida adotadas pelos estudantes de ensino médio podem ser relacionadas a baixos índices de estresse nesta fase.

BACKGROUND: High school students deal with stressful experiences at this stage, such as the choice of which profession to follow and the training for admission exams, which may predispose to the development of Burnout Syndrome. AIM: This study aimed to verify the correlation between Burnout Syndrome and the lifestyle of high school students. METHODS: This is a cross-sectional and quantitative study performed with high school students. In order to collect data, we used the questionnaire Fantastic Lifestyle and Maslach Burnout Inventory . In order to estimate and test the association between the dimensions of Burnout Syndrome and the lifestyle among students, we used the Pearson s correlation coefficient. RESULTS: This study was attended by 966 students, with a mean age of 16.61 years. We observed that 12.1% presented signs of Burnout Syndrome. We observed a negative relationship among emotional exhaustion and sleep, seatbelt, stress and safe sex (-0.225; p <0.001); disbelief and cigarettes and drugs (-0.206; p <0.001); and positive for professional effectiveness, family and friends (0.224; p <0.001), sleep, seatbelt, stress and safe sex (0.282; p <0.001) and introspection (0.245; p <0.001). CONCLUSION: We can be conclude that healthy life practices adopted by high school students can be related to low stress indexes at this stage.

RESUMO: Neste trabalho, objetivou-se avaliar a eficiência dos corantes artificiais, amarelo crepúsculo e vermelho bordeaux S, e o uso do glicerol em diferentes concentrações, na montagem de lâminas com esporos de Oidium sp., Albugo ipomoeae-panduratae, Pochonia chlamydosporia e hifas de Phytopythium helicoides. Foram avaliadas as concentrações de 0,5, 1,0, 1,5, 2,0, 3,0 e 5,0% (p/v) do produto comercial da mistura dos corantes artificiais e adição de glicerol nas concentrações de 0,25, 0,5 e 1,0%. Para coloração de clamidósporos, a suspensão foi colocada na solução corante ou aquecida a 80ºC por 5 minutos e as lâminas preparadas com líquido de montagem. A partir da concentração de 2% da mistura dos corantes houve maior coloração dos esporos. A adição de glicerol na solução corante melhorou o contraste dos esporângios, hifas e clamidósporos. Maior intensidade e uniformidade de coloração de clamidósporos ocorreram na solução corante 3% e glicerol 1% aquecida, em comparação com a solução sem aquecimento e azul-de-algodão.

ABSTRACT: The objective of this research was to evaluate the efficiency of artificial dyes, sunset yellow and red bordeaux S, and the use of glycerol in different concentrations to consistently stain fungal structures in slides containing spores of Oidium sp., Albugo ipomoeae-panduratae, Pochonia chlamydosporia and hyphae of Phytopythium helicoides. Commercial product mixtures of the artificial dyes at 0.5, 1.0, 1.5, 2.0, 3.0 and 5.0% (w/v) added with glycerol at 0.25, 0.5 and 1.0% were evaluated. To stain chlamydospores, the suspension was placed in the staining solution or heated at 80ºC for 5 minutes. The slides were prepared by the wet mount slide method. Fungal spores were consistently stained starting at a concentration of 2% of the staining solution. The addition of glycerol to the staining solution improved the contrast of the sporangia, hyphae and chlamydospores. Higher intensity and uniformity of chlamydospore’s staining was verified using 3% dye solution and 1% heated glycerol, when compared to the unheated and blue-cotton solution.

Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in liver ischemia/reperfusion injury in rats. Methods: 25 male rats (Wistar) were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of liver ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during liver ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); Remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. Results: Per+HSS group showed a lower degree of liver dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly improved liver function and reduced histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute liver injury induced by ischemia/reperfusion.

PURPOSE: To evaluate the effects of nitrofurazone on the correction of abdominal wall defect treated with polypropylene mesh involved by fibrous tissue in rats. METHODS: A defect in the abdominal wall was created and corrected with polypropylene mesh in 20 rats. They were randomly distributed into four groups: control, fibrous mesh, nitrofurazone and nitrofurazone dip in the mesh. Euthanasia was performed in 21 post-operative days. The healing process was analyzed regarding the meshes and macroscopic and microscopic aspects. RESULTS: All animals had adhesions. However, no statistically significant difference (p>0.05) when compared between groups. Similarly microscopic analysis, in which there was no statistical significance level for the evaluated parameters such as mono and polymorphonuclear lymphocytes, granuloma, fibrosis, necrosis and collagen proliferation. CONCLUSION: There was no significant effect on the abdominal wall defect repair with polypropylene mesh surrounded by fibrous tissue when dipped in nitrofurazone 2%.