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DNA rare copy number alterations in Reinke’s Edema
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Móz, Luis Eduardo Silva

; Martins, Regina Helena Garcia

; Lapa, Rainer Marco Lopez

; Villacis, Rolando André Rios

; Reis, Patricia Pintor dos

; Rogatto, Silvia Regina

Brazilian Journal of Otorhinolaryngology

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Abr 2023, Volume 89 Nº 2 Páginas 279 - 284

Resumo: En | Texto: En | PDF: En

Abstract Introduction: Reinke’s Edema (RE) is a laryngeal lesion related to excessive tobacco smoking, voice overuse, and laryngopharyngeal reflux. Although the risk of malignancy has been considered low in literature, RE is classified among precancerous lesions. Objectives: We investigated DNA Copy Number Alterations (CNAs) in specimens of RE and its potential association with malignant progression. Methods: We used array-based comparative genomic hybridization (aCGH, Agilent 4 × 180 K platform) to study eight RE cases. All patients were heavy tobacco users for at least 30 years, and none of them progressed to cancer in the follow-up (>8 years). Two RE presented mild dysplasia, one moderate dysplasia, and no histological alterations were found in the remaining five cases. CNAs were compared with the Database of Genomic Variants (DGV) and genes mapped on altered regions had their functions annotated. Results: Six of eight patients showed different rare copy number alterations on chromosomes 2q37.3, 4q13.1, 4q13.3, 7q11.22, 10p14, and 13q34. A gain of the whole chromosome 8 were detected in one case. Of interest, four of eight RE cases showed copy number imbalances involving genes previously described in several tumor types (RASA3, COL6A3, LINC00707, LINP1, SMR3A, and SMR3B). Conclusion: The genomic imbalances herein found in RE have the potential to contribute to the phenotype but with limited or no risk of cancer. A long-term follow-up in a large series of patients could clarify the mechanisms involved in the malignant progression of RE. Level of evidence: 4.

https://doi.org/10.1016/j.bjorl.2022.09.002

Maria Ester de Freitas beyond pioneerism: critique and “Brasilidade” in organization studies
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Cavalcanti, Maria Fernanda Rios

; Silva, Andre Luis

Cadernos EBAPE.BR

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2023, Volume 21 Nº 6 elocation e2022-0266

Resumo: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract Our objective is to investigate and highlight the contributions of Maria Ester de Freitas to the development of the Brazilian field of Management and Organization Studies (MOS). Ester wrote pioneering work in the development of impacting themes in this field - such as organizational culture, intercultural management, diversity, and moral harassment. This Global South scholar’s approach to such themes was shaped by a peculiar criticism that found an echo amongst Brazilian scholars and practitioners. Ester knew how to produce and reproduce complex and often controversial topics courageously, with intellectual refinement and an impressive cultural repertoire embedded in elements of “Brasilidade.” Ester’s main concern was to think about the human condition in organizations and, therefore, to promote the social responsibility of organizations, a concern that continues to demand urgent reflection. Hence, this research question is: in addition to being a pioneer in developing such themes in the Global South, which elements keep Ester’s work relevant today for organization studies in general? We conclude that Ester’s relevance extrapolates the relevance of the topics she addressed, as her academic style is marked by correlating criticism and manifestations of “Brasilidade”. This correlation appears in a kind of “Becoming-Ester” throughout her texts and speeches, an investigative and provocative posture that invites us to reflect and broaden our horizons when approaching our local realities. Finally, we show that the content and style of Ester’s legacy instigates alternative ways to critically reflect on the human condition in organizations through unconventional and unprejudiced eyes, thus, fostering new approaches to imagining and creating more socially responsible organizations.

Resumen Nuestro objetivo es investigar y resaltar las contribuciones de Maria Ester de Freitas para el desarrollo del campo brasileño de Estudios Organizacionales. Ester escribió obras pioneras en el desarrollo de temas de impacto en esta área, como la cultura organizacional, la gestión intercultural, la diversidad y el acoso moral. El estilo de abordar estos temas de esta académica del Sur global fue moldeado por una crítica peculiar que resonó entre los académicos y profesionales brasileños. Ester supo producir y reproducir valientemente temas complejos y a menudo controvertidos, con refinamiento intelectual y un repertorio cultural impresionante embutido en elementos de la “brasilidad”. La principal preocupación de Ester fue pensar la condición humana en las organizaciones y, por tanto, promover la responsabilidad social de las organizaciones, preocupación que sigue exigiendo una reflexión urgente. De esta manera, la pregunta que queremos responder es: además de ser pionera en el desarrollo de tales temas en el Sur global, ¿qué elementos hacen que el trabajo de Ester hoy continúe siendo relevante para los estudios organizacionales en general? Concluimos que la relevancia de Ester va más allá de la relevancia de los temas que aborda, ya que su estilo académico está marcado por correlatos críticos y expresiones de “brasilidad”. Esta correlación aparece en una especie de “Devenir-Ester” a lo largo de sus textos y discursos, una postura indagatoria y provocadora que nos invita a reflexionar y ampliar nuestros horizontes al acercarnos a nuestras realidades locales. Finalmente, mostramos que el contenido y el estilo del legado de Ester instigan formas alternativas para reflexionar críticamente sobre la condición humana en organizaciones con una mirada no convencional y desprejuiciada, fomentando así nuevos enfoques para imaginar y crear organizaciones más socialmente responsables.

Resumo Nosso objetivo é investigar e destacar as contribuições de Maria Ester de Freitas para o desenvolvimento do campo brasileiro de estudos organizacionais e de gestão. Ester escreveu trabalhos pioneiros no desenvolvimento de temas impactantes nessa área - como cultura organizacional, gestão intercultural, diversidade e assédio moral. O estilo dessa estudiosa do Sul Global em abordar tais temas foi moldado por uma crítica peculiar que encontrou eco entre estudiosos e profissionais brasileiros. Ester soube produzir e reproduzir temas complexos e muitas vezes polêmicos de forma corajosa, com requinte intelectual e um impressionante repertório cultural envolvidos em elementos da “brasilidade”. A principal preocupação de Ester foi pensar a condição humana nas organizações e, por isso, promover a responsabilidade social das organizações, preocupação que continua a exigir uma reflexão urgente. Assim, a pergunta que queremos responder é: além de ter sido pioneira no desenvolvimento de tais temas no Sul Global, quais elementos mantêm a obra de Ester relevante hoje para os estudos organizacionais em geral? Concluímos que a relevância de Ester extrapola a relevância dos temas por ela abordados, pois seu estilo acadêmico é marcado por perspectivas críticas e manifestações de “brasilidade”. Essa correlação aparece em uma espécie de “Devir-Ester”, ao longo de seus textos e falas, consolidando uma postura investigativa e provocativa que nos convida a refletir e ampliar horizontes ao abordar nossas realidades locais. Por fim, mostramos que o conteúdo e o estilo do legado de Ester instigam caminhos alternativos para refletir criticamente sobre a condição humana nas organizações com um olhar não convencional e sem preconceitos, fomentando assim novas abordagens para imaginar e criar organizações mais socialmente responsáveis.

https://doi.org/10.1590/1679-395120220266x

Quantitative Metallographic Parameters to Describe Microstructures of Multiphase Materials
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Alves, André Luiz Moraes

; Assis, Weslley Luiz da Silva

; Rios, Paulo Rangel

Materials Research

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2023, Volume 26 elocation e20220476

Resumo: En | Texto: En | PDF: En

Abstract One typically characterizes the transformation kinetics of a parent phase, α, into a single phase, β, by measuring the volume fraction transformed, V V β, against time. Sometimes one also reports the interfacial area density between the new and the parent phase, S V α β, against the volume fraction transformed. S V α β is a dynamic interface. It migrates as the growth of the new phase takes place. Interfaces between transformed phases might be called static interfaces. These may be present before transformation starts, for example, grain boundaries of a polycrystalline parent phase. Alternatively, static interfaces, S V β β , may appear during the transformation because of impingement. Therefore, one may better understand the microstructural evolution following the behavior of the volume fraction, dynamic and static interfaces. A more complicated situation occurs if the parent phase transforms into two or more product phases, for example, α→β,γ. In this work, we apply parameters to describe the transformation of a parent phase into a single phase, the contiguity and the dispersion, to the situation in which the parent phase transforms into two or more phases. We tested these parameters against computer simulations and concluded that they combine a good description of the behavior of the simulated transformations and simplicity.

https://doi.org/10.1590/1980-5373-mr-2022-0476

Ecologia da comunidade arbórea de Cerrado stricto sensu às margens de rodovias
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Rios, Jovan Martins

; Santos, Lilian Cristina da Silva

; Costa, João Paulo

; Pereira, Ismael Martins

; Gusson, André Eduardo

; Vale, Vagner Santiago do

Ciência Florestal

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2023, Volume 33 Nº 2 elocation e62683

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT The construction of highways is a major threat to biodiversity the Cerrado. From the phytosociological analyzes in the non aedificandi ranges, this work evaluated its conservation status of cerrado vegetation near highways. The study was carried out in four regions of Cerrado: northwest (NO-GO), south (S-GO) and southeast of Goiás State (SE-GO), and in the Triângulo Mineiro and Alto do Paranaíba in Minas Gerais (TRI). All tree individuals with C30cm (circunference measuredat 30 cm of soil height) > 15 cm, present in 100 plots (50 m x 10 m) independently arranged. The survey showed 1663 individuals distributed in 163 species and 44 families. The density for the regions was 504.44, 395, 307.69 and 151.03 ha-1. The highest similarity on the basis of the Jaccard and Morisita-Horn indices occurred between the TRI and SE-GO areas (0.340 and 0.550), while the lowest values were between the northwest (NO-GO) and south (S-GO) of Goiás State (0.086 and 0.159). Of the species found on the roadsides, 76.3% were also found in conserved areas. The vegetated strips along the highways form small habitats capable of sustaining a diversity similar to that found in natural areas, yet these environments suffer from anthropic disturbances favoring the occurrence of unusual species of that native community.

RESUMO A construção de rodovias representa uma das principais ameaças à biodiversidade do Cerrado. A partir de análises fitossociológicas nas faixas non aedificandi (sem edificação), avaliou-se o estado de conservação da vegetação de cerrado localizadas nas margens de rodovias. O estudo foi realizado em quatro regiões do Cerrado brasileiro: noroeste (NO-GO), sul (S-GO) e sudeste de Goiás (SE-GO), e no Triângulo Mineiro e Alto do Paranaíba em Minas Gerais (TRI). Foram inventariados todos os indivíduos arbóreos com C30cm (circunferência mensurada a 30 cm de altura do solo) > 15 cm presentes em 100 parcelas (50 m x 10 m) dispostas de maneira independente. O levantamento amostrou 1663 indivíduos distribuídos em 163 espécies e 44 famílias. A densidade para as regiões foi de 504,44, 395, 307,69 e 151,03 ha-1. A maior similaridade, com base nos índices de Jaccard e Morisita-Horn, ocorreu entre as áreas TRI e SE-GO (0,340 e 0,550), enquanto o menor valor entre as áreas a noroeste (NO-GO) e sul (S-GO) de Goiás (0,086 e 0,159). Das espécies encontradas nas margens de rodovias, 76,3% foram encontradas também em áreas conservadas. As faixas vegetadas nas margens das rodovias formam pequenos habitats capazes de sustentar uma diversidade semelhante à encontrada em áreas naturais, no entanto, esses ambientes sofrem com perturbações antrópicas, favorecendo a ocorrência de espécies incomuns daquela comunidade nativa.

https://doi.org/10.5902/1980509862683

Multi-level toxicity assessment of the antidepressant venlafaxine in embryos/larvae and adults of zebrafish (Danio rerio)
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Oliveira, Ana Clara

; Fascineli, Maria Luiza

; Oliveira, Paula Martins de

; Gelfuso, Guilherme Martins

; Villacis, Rolando André Rios

; Grisolia, Cesar Koppe

Genetics and Molecular Biology

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2023, Volume 46 Nº 3 elocation e20220377

Resumo: En | Texto: En | PDF: En

Abstract The toxic effects of venlafaxine (VLX) on aquatic organisms have already been verified and therefore are a proven matter of concern. Herein, we evaluated zebrafish embryos/adults after acute exposure to VLX. Embryos/larvae were exposed to different concentrations of VLX (100-1000 mg/L; 1.33 as a dilution factor), to evaluate mortality/developmental changos and to analyze biomarkers (0.002-100 mg/L). For adults, mortality, genotoxicity, and biomarkers were assessed in five different concentrations of VLX (1-100 mg/L). The median lethal concentration (LC50-168h) was 274.1 mg/L for embryos/larvae, and >100 mg/L for adults (LC50-96h). VLX decreased the heart rate frequency and caused premature hatching and lack of equilibrium in embryos/larvae exposed to different concentrations ranging from 100 to 562.5 mg/L. The activity of acetylcholinesterase (AChE) was inhibited in larvae exposed to 1, 25 and 100 mg/L. Glutathione-S-transferase (GST) activity was reduced in both larvae and adults after exposure to different concentrations, mainly at 25 mg/L. For both larvae and adults, lactate dehydrogenase (LDH) activity increased after 100 mg/L of VLX exposure. No DNA damage was observed in peripheral erythrocytes. Exposure to VLX may cause adverse effects on zebrafish in their early and adult life stages, interfering with embryo-larval development, and can induce physiological disturbances in adults.

https://doi.org/10.1590/1678-4685-gmb-2022-0377

O que mudou entre os períodos de pico e de platô durante a primeira onda do SARS-CoV-2? Estudo multicêntrico português em unidades de cuidados intensivos
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Pereira, Rui Antunes

; Sousa, Marta

; Cidade, José Pedro

; Melo, Luís

; Lopes, Diogo

; Ventura, Sara

; Aragão, Irene

; Lima Neto, Raul Miguel de Freitas

; Molinos, Elena

; Marques, Ana

; Cardoso, Nelson

; Marino, Flávio

; Monteiro, Filipa Brás

; Oliveira, Ana Pinho

; Silva, Rogério C

; Real, André Miguel Neto

; Banheiro, Bruno Sarmento

; Reis, Renato

; Adão-Serrano, Maria

; Cracium, Ana

; Valadas, Ana

; Ribeiro, João Miguel

; Póvoa, Pedro

; Tapadinhas, Camila

; Mendes, Vítor

; Coelho, Luís

; Maia, Raquel

; Freitas, Paulo Telles

; Ferreira, Isabel Amorim

; Ramires, Tiago

; Val-Flores, Luís Silva

; Cascão, Mariana

; Alves, Rita

; Rodeia, Simão C

; Barrigoto, Cleide

; Cardiga, Rosa

; Silva, Maria João Ferreira da

; Vale, Bruno

; Fonseca, Tatiana

; Rios, Ana Lúcia

; Camões, João

; Pérez, Danay

; Cabral, Susana

; Ribeiro, Maria Inês

; Mendes, João João

; Gouveia, João

; Fernandes, Susana Mendes

Revista Brasileira de Terapia Intensiva

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Dez 2022, Volume 34 Nº 4 Páginas 433 - 442

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: To analyze and compare COVID-19 patient characteristics, clinical management and outcomes between the peak and plateau periods of the first pandemic wave in Portugal. Methods: This was a multicentric ambispective cohort study including consecutive severe COVID-19 patients between March and August 2020 from 16 Portuguese intensive care units. The peak and plateau periods, respectively, weeks 10 - 16 and 17 - 34, were defined. Results: Five hundred forty-one adult patients with a median age of 65 [57 - 74] years, mostly male (71.2%), were included. There were no significant differences in median age (p = 0.3), Simplified Acute Physiology Score II (40 versus 39; p = 0.8), partial arterial oxygen pressure/fraction of inspired oxygen ratio (139 versus 136; p = 0.6), antibiotic therapy (57% versus 64%; p = 0.2) at admission, or 28-day mortality (24.4% versus 22.8%; p = 0.7) between the peak and plateau periods. During the peak period, patients had fewer comorbidities (1 [0 - 3] versus 2 [0 - 5]; p = 0.002) and presented a higher use of vasopressors (47% versus 36%; p < 0.001) and invasive mechanical ventilation (58.1 versus 49.2%; p < 0.001) at admission, prone positioning (45% versus 36%; p = 0.04), and hydroxychloroquine (59% versus 10%; p < 0.001) and lopinavir/ritonavir (41% versus 10%; p < 0.001) prescriptions. However, a greater use of high-flow nasal cannulas (5% versus 16%, p < 0.001) on admission, remdesivir (0.3% versus 15%; p < 0.001) and corticosteroid (29% versus 52%, p < 0.001) therapy, and a shorter ICU length of stay (12 days versus 8, p < 0.001) were observed during the plateau. Conclusion: There were significant changes in patient comorbidities, intensive care unit therapies and length of stay between the peak and plateau periods of the first COVID-19 wave.

RESUMO Objetivo: Analisar e comparar as características de pacientes críticos com a COVID-19, a abordagem clínica e os resultados entre os períodos de pico e de platô na primeira onda pandêmica em Portugal. Métodos: Este foi um estudo de coorte multicêntrico ambispectivo, que incluiu pacientes consecutivos com a forma grave da COVID-19 entre março e agosto de 2020 de 16 unidades de terapia intensiva portuguesas. Definiram-se as semanas 10 - 16 e 17 - 34 como os períodos de pico e platô. Resultados: Incluíram-se 541 pacientes adultos com mediana de idade de 65 [57 - 74] anos, a maioria do sexo masculino (71,2%). Não houve diferenças significativas na mediana de idade (p = 0,3), no Simplified Acute Physiology Score II (40 versus 39; p = 0,8), na pressão parcial de oxigênio/fração inspirada de oxigênio (139 versus 136; p = 0,6), na terapia com antibióticos na admissão (57% versus 64%; p = 0,2) ou na mortalidade aos 28 dias (24,4% versus 22,8%; p = 0,7) entre o período de pico e platô. Durante o período de pico, os pacientes tiveram menos comorbidades (1 [0 - 3] versus 2 [0 - 5]; p = 0,002); fizeram mais uso de vasopressores (47% versus 36%; p < 0,001) e ventilação mecânica invasiva na admissão (58,1% versus 49,2%; p < 0,001), e tiveram mais prescrição de hidroxicloroquina (59% versus 10%; p < 0,001), lopinavir/ritonavir (41% versus 10%; p < 0,001) e posição prona (45% versus 36%; p = 0,04). Entretanto, durante o platô, observou-se maior uso de cânulas nasais de alto fluxo (5% versus 16%; p < 0,001) na admissão, remdesivir (0,3% versus 15%; p < 0,001) e corticosteroides (29% versus 52%; p < 0,001), além de menor tempo de internação na unidade de terapia intensiva (12 versus 8 dias; p < 0,001). Conclusão: Houve mudanças significativas nas comorbidades dos pacientes, nos tratamentos da unidade de terapia intensiva e no tempo de internação entre os períodos de pico e platô na primeira onda da COVID-19.

https://doi.org/10.5935/0103-507x.20210037-pt

EXPERIMENTO DIDÁTICO DE QUIMIOMETRIA EMPREGANDO IMAGENS DIGITAIS OBTIDAS POR CELULAR PARA DETERMINAR ADULTERAÇÃO DE AZEITE DE OLIVA COM ÓLEO DE SOJA: UM TUTORIAL, PARTE VI
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Rios, Carlos Alberto

; Souza, André Marcelo de

; Poppi, Ronei Jesus

; Breitkreitz, Márcia Cristina

Química Nova

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Set 2022, Volume 45 Nº 9 Páginas 1167 - 1176

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The aim of this manuscript was to show the concepts involving data treatment of RGB based image analysis by univariate and multivariate approaches - the latter using Partial Least Squares (PLS) - using as a practical example application the determination of extravirgin olive oil adulteration. Digital images were collected using a device made of LEDs, batteries and a smartphone and results are shown in a tutorial format using Matlab computing environment. The experiment can serve as an example for teaching the subject for undergraduate and graduate students.

https://doi.org/10.21577/0100-4042.20170946

Guidelines on COVID-19 vaccination in patients with immune-mediated rheumatic diseases: a Brazilian Society of Rheumatology task force
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Tavares, Anna Carolina Faria Moreira Gomes

; Melo, Ana Karla Guedes de

; Cruz, Vítor Alves

; Souza, Viviane Angelina de

; Carvalho, Joana Starling de

; Machado, Ketty Lysie Libardi Lira

; Valadares, Lilian David de Azevedo

; Reis Neto, Edgard Torres dos

; Rezende, Rodrigo Poubel Vieira de

; Guimarães, Maria Fernanda Brandão de Resende

; Ferreira, Gilda Aparecida

; Braz, Alessandra de Sousa

; Vieira, Rejane Maria Rodrigues de Abreu

; Pinheiro, Marcelo de Medeiros

; Ribeiro, Sandra Lúcia Euzébio

; Bica, Blanca Elena Gomes Rios

; Baptista, Kátia Lino

; Costa, Izaias Pereira da

; Marques, Claudia Diniz Lopes

; Lopes, Maria Lúcia Lemos

; Martinez, José Eduardo

; Giorgi, Rina Dalva Neubarth

; Mota, Lícia Maria Henrique da

; Loures, Marcos Antônio Araújo da Rocha

; Paiva, Eduardo dos Santos

; Monticielo, Odirlei André

; Xavier, Ricardo Machado

; Kakehasi, Adriana Maria

; Pileggi, Gecilmara Cristina Salviato

Advances in Rheumatology

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2022, Volume 62 elocation 3

Resumo: En | Texto: En | PDF: En

Abstract Objective: To provide guidelines on the coronavirus disease 2019 (COVID-19) vaccination in patients with immune-mediated rheumatic diseases (IMRD) to rheumatologists considering specific scenarios of the daily practice based on the shared-making decision (SMD) process. Methods: A task force was constituted by 24 rheumatologists (panel members), with clinical and research expertise in immunizations and infectious diseases in immunocompromised patients, endorsed by the Brazilian Society of Rheumatology (BSR), to develop guidelines for COVID-19 vaccination in patients with IMRD. A consensus was built through the Delphi method and involved four rounds of anonymous voting, where five options were used to determine the level of agreement (LOA), based on the Likert Scale: (1) strongly disagree; (2) disagree, (3) neither agree nor disagree (neutral); (4) agree; and (5) strongly agree. Nineteen questions were addressed and discussed via teleconference to formulate the answers. In order to identify the relevant data on COVID-19 vaccines, a search with standardized descriptors and synonyms was performed on September 10th, 2021, of the MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, ClinicalTrials.gov, and LILACS to identify studies of interest. We used the Newcastle–Ottawa Scale to assess the quality of nonrandomized studies. Results: All the nineteen questions-answers (Q&A) were approved by the BSR Task Force with more than 80% of panelists voting options 4—agree—and 5—strongly agree—, and a consensus was reached. These Guidelines were focused in SMD on the most appropriate timing for IMRD patients to get vaccinated to reach the adequate covid-19 vaccination response. Conclusion: These guidelines were developed by a BSR Task Force with a high LOA among panelists, based on the literature review of published studies and expert opinion for COVID-19 vaccination in IMRD patients. Noteworthy, in the pandemic period, up to the time of the review and the consensus process for this document, high-quality evidence was scarce. Thus, it is not a substitute for clinical judgment.

https://doi.org/10.1186/s42358-022-00234-7

Scrotal hemangiosarcoma in a Large White boar
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Silveira, Renato Luiz

; Cruz, Ana Claudia de Menezes

; Doria, Phillipe Bauer de Araújo

; Silveira, Joao Marcelo Silva

; Vasconcelos, Carlos Otávio de Paula

; Rodrigues, André Luis Rios

; Arashiro, Eduardo Kenji Nunes

; Jerdy, Hassan

; Dias, Mariana Tavares

; Carvalho, Eulógio Carlos Queiroz de

; Costa, Carlos Henrique Campello

Ciência Rural

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2022, Volume 52 Nº 4 elocation e20210157

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Na espécie suína os tumores são pouco diagnosticados devido a curta vida útil dos animais de produção. Normalmente não causam sinais clínicos, sendo, muitas vezes, encontrados no momento do abate. Um reprodutor com dois anos de idade, da raça Large White, utilizado no manejo reprodutivo de uma granja, sem histórico de problemas pré-existentes, foi examinado em função de lesões na pele da bolsa escrotal. Foram coletadas amostras de segmentos de pele contendo lesões para o diagnóstico histopatológico e imunohistoquímico. Microscopicamente os nodos em bolsa escrotal consistiam em neoplasias mal demarcadas, altamente celulares, expansivas e multifocalmente invasivas composta por endoteliócitos imaturos organizados em formações neovasculares. As células tumorais eram pleomórficas, levemente ovais a fusiformes com citoplasma eosinofílico e núcleos hipercromáticos e com um a três nucléolos. Todos os nódulos analisados foram compatíveis com hemangiossarcoma. Para a quantificação da angiogênese tecidual, após a avaliação imunohistoquímica, as células neoplásicas imunoexpressaram os anticorpos monoclonais CD31 e Fator VIII, através da identificação de proteínas expressas na superfície das células endoteliais. Observou-se o marcador de proliferação celular Ki67 positivo em aproximadamente 10% das células neoplásicas, demonstrando um alto grau de malignidade. O hemangiossarcoma na espécie suína já foi identificado em vários órgãos e tecidos, mas, até o momento, nenhum trabalho demonstrou o diagnóstico na pele da bolsa escrotal, como reportado, ineditamente, neste estudo. Face o exposto, espera-se que este relato contribua para a frequência de neoplasias encontradas na espécie suína.

ABSTRACT: Tumors are rarely diagnosed in swine specie because of the short lifespan of production animals. Normally, these tumors do not present any clinical signs and are often detected at the time of slaughter. A 2-year-old Large White boar, used in the reproductive management of a farm and without a history of pre-existing problems, was examined for skin lesions on the scrotum. Samples were collected from skin segments containing lesions for histopathological and immunohistochemical diagnosis. Microscopically, the nodes in the scrotum pouch consisted of poorly demarcated, highly cellular, expansile, and multifocally invasive neoplasms, composed of immature endotheliocytes organized into neovascular formations. The tumor cells were pleomorphic, slightly oval to spindle-shaped, with eosinophilic cytoplasm and hyperchromatic nuclei with one to three nucleoli. All the nodules analyzed were compatible with hemangiosarcoma. After immunohistochemical evaluation, for the quantification of tissue angiogenesis the neoplastic cells immunoexpressed the CD31 monoclonal antibodies and factor VIII, through the identification of proteins expressed on the surface of endothelial cells. The Ki67 cell proliferation marker was positive in approximately 10% of the neoplastic cells, demonstrating a high degree of malignancy. Hemangiosarcoma in swine species has already been identified in several organs and tissues; however, to date, no study has demonstrated the diagnosis of this condition on the skin of the scrotum, as reported in this study. Therefore, it is expected that this report will contribute to the knowledge of the frequency of neoplasms in swine species.

https://doi.org/10.1590/0103-8478cr2021015 69 downloads

10.

Quality of clinical studies present in the package inserts of coagulation factors used in the treatment of hemophilia
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Araújo, Yasmin Gonçalves

; Paolinelli, João Pedro Vasconcelos

; Pichitelli, Janaina Souza Dias

; Rios, Danyelle Romana Alves

; Baldoni, Nayara Ragi

; Baldoni, André Oliveira

einstein (São Paulo)

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2022, Volume 20 elocation eAO6859

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective To identify and analyze the quality of scientific evidence from clinical efficacy studies present in the package inserts of coagulation factors, used in the treatment of hemophilia A and B. Methods Documentary study developed in two stages. The first stage consisted of identifying the medicine packages inserts electronically registered in the Brazilian Health Regulatory Agency, and analyzing the availability of the bibliographic references cited therein. This analysis was conducted in the PubMed, SciELO, Google Scholar, and Web of Science databases. The second step was the analysis of the methodological quality of the efficacy studies. Two trained researchers used the Cochrane Collaboration Risk of Bias version 5.1.0 tools for methodological quality analysis, and Review Manager 5.4 software to generate the risk of bias graph. Results Of the 17 medicines listed, 7 had referenced package inserts. Of these, 10 studies were eligible for analysis of methodological quality. More than half of the analyzed studies did not control for selection, performance, and detection bias. A total of 100% controlled attrition and reporting biases, and 50% had a high risk of conflict of interest. Conclusion The biases present are significant and may have influenced the overestimation of the effects of the outcomes of each of the studies.

https://doi.org/10.31744/einstein_journal/2022ao6859

11.

Fatal Brazilian spotted fever in a healthy military man during field training in Rio de Janeiro city, southeastern Brazil
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Pacheco-Silva, Ana Beatriz

; Martins, Ezequias Batista

; López, Angie Jeannine Rios

; Detepo, Paula João Tomas

; Mamani, Roxana Flores

; Japiassú, André Miguel

; Lupi, Otilia

; Mendes, Tulio Vieira

; Pina-Costa, Anielle de

; Calvet, Guilherme Amaral

; Bressan, Clarisse da Silveira

; Silva, Michele Fernanda Borges da

; Ogino, Liana Lumi

; Assis, Matheus Ribeiro da Silva

; Marques, Amanda Meneleu

; Dias, Cristina Maria Giordano

; Borchert, Juliana Mildward

; Lemos, Elba Regina Sampaio de

; Brasil, Patrícia

Revista do Instituto de Medicina Tropical de São Paulo

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2022, Volume 64 elocation e77

Resumo: En | Texto: En | PDF: En

ABSTRACT Brazilian spotted fever, a zoonotic disease transmitted by ticks, is caused by Rickettsia rickettsii. We report a fulminant case of this zoonosis in a healthy 46-year-old military man in the urban region of Rio de Janeiro city, in October, 2021. Ticks and capybaras (Amblyomma sculptum, Hydrochoerus hydrochaeris, respectively) were identified in the military fields, pointing to the participation of this large synanthropic rodent, recognized as an efficient amplifier host of Rickettsia rickettsii in Brazil. As the military population is considered a risk group for spotted fever, it is necessary to alert health professionals to the importance of the early detection of the disease and its adequate management, mainly in populations that are particularly at risk of exposure to ticks, in order to avoid fatal outcomes.

https://doi.org/10.1590/s1678-9946202264077

12.

Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows
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Carvalheira, Luciano de Rezende

; Santos, Gustavo Bervian dos

; Jasmim,

; Guimarães, Clóvis Ribeiro

; Campos, Mariana Magalhães

; Machado, Fernanda Samarini

; Pedroso, Alexandre Mendonça

; Silva, Tadeu Eder da

; Nogueira, Luiz Altamiro Garcia

; Rodrigues, André Luís Rios

; Carvalho, Bruno Campos de

Animal Reproduction

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2022, Volume 19 Nº 3 elocation e20210088

Resumo: En | Texto: En | PDF: En

Abstract This study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumen-undegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes’ DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes’ DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes’ embryo development impacting fertility of lactating dairy cows.

https://doi.org/10.1590/1984-3143-ar2021-0088

13.

Here comes the large catfish “jaú” Zungaro jahu (Ihering 1898) (Teleostei, Pimelodidae): a new alarming case of fish introduction in a high-endemism Neotropical ecoregion
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Frota, Augusto

; Abilhoa, Vinícius

; Freitas, Matheus Oiveira

; Meyer, Rodrigo Lira

; Gonçalves, Eduardo Rios

; Azevedo, Filipe Manoel

; Gubiani, Éder André

; Graça, Weferson Júnio da

Acta Limnologica Brasiliensia

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2022, Volume 34 elocation e25

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: Objetivo Espécies de peixes não nativas têm sido intimamente relacionadas aos sérios danos à biodiversidade aquática devido aos efeitos negativos sobre a fauna nativa. Nosso objetivo é relatar a primeira e alarmante ocorrência do bagre não nativo “jaú” Zungaro jahu na bacia do rio Iguaçu acima das Cataratas do Iguaçu, uma ecorregião neotropical com alto endemismo de peixes. Métodos As amostragens dos peixes foram realizadas com rede de emalhar de fundo no Reservatório de Segredo, onde foram anestesiados em cloridrato de benzocaína e fixados em formol em campo, posteriormente foram identificados em laboratório e depositados no Museu de História Natural do Capão da Imbuia (MHNCI). Resultados Nossas amostragens recentes na bacia do rio Iguaçu registraram um indivíduo não nativo do bagre “jaú” Zungaro jahu no Reservatório de Segredo. Conclusões Pretendemos, principalmente, alertar sobre o risco de expansão da ocorrência desta espécie não nativa ao longo da cascata de reservatórios do rio Iguaçu, onde potencialmente poderá desenvolver sérios danos à cadeia trófica, predação de espécies endêmicas de pequeno porte e competição, especialmente nos locais onde o pimelodídeo endêmico e ameaçado de extinção “surubim-do-iguaçu” (Steindachneridion melanodermatum) mantém populações viáveis, atualmente restrita à bacia do Baixo rio Iguaçu, exibindo sinais recentes do efeito de gargalo.

Abstract: Aim Non-native fish species have been closely related to serious damage to aquatic biodiversity due to their negative effects on native fauna. We aim to report the first and alarming occurrence of the non-native catfish “jaú” Zungaro jahu in the Iguaçu River basin above the Iguaçu Falls, a high-endemism Neotropical ecoregion for fish. Methods Fish samplings were taken with a bottom gill net in the Segredo Reservoir, where they were anesthetized in benzocaine hydrochloride and fixed in formaldehyde in the field, later identified in the laboratory and housed at the Museu de História Natural do Capão da Imbuia (MHNCI). Results Our recent samplings in the Iguaçu River basin recorded one non-native individual of the catfish “jaú” Zungaro jahu in the Segredo Reservoir. Conclusions We mainly intend to alarm about the risk of expansion of the occurrence of this non-native species throughout the cascading reservoirs in the Iguaçu River where it could potentially develop serious damage to the trophic chain, predation of small-sized endemic species, and competition, especially in the sites where the endemic and endangered pimelodid “surubim-do-iguaçu” (Steindachneridion melanodermatum) maintains viable populations, currently restricted to the Lower Iguaçu River basin exhibiting recent signs of a population bottleneck.

https://doi.org/10.1590/s2179-975x4322

14.

Control of the fungi Lasiodiplodia theobromae, the causal agent of dieback, in cv. syrah grapevines
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Rusin, Carine

; Cavalcanti, Fábio Rossi

; Lima, Patrícia Carla Giloni de

; Faria, Cacilda Márcia Duarte Rios

; Almança, Marcus André Kurtz

; Botelho, Renato Vasconcelos

Acta Scientiarum. Agronomy

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2021, Volume 43 elocation e44785

Resumo: En | Texto: En | PDF: En

ABSTRACT. Grapevine trunk diseases are among the most important limiting factors of worldwide viticulture. In this context, we aimed to verify the effect of chemical fungicides, biological agents and plant extracts on the control of Lasiodiplodia theobromae in pruning wounds and their physiological effects in cv. Syrah grapevines. Plant extracts (clove, cinnamon, garlic, rosemary and lemongrass), commercial fungicides (difeconazole, tebuconazole, mancozeb, sulfur, pyraclostrobin, fosetyl-Al, and azoxystrobin), chitosan, Trichoderma harzianum and Bacillus subtilis were used for the in vitro trials. The protection of pruning wounds in the potted vines was studied using fosetyl-Al, tebuconazole, Trichoderma harzianum, Bacillus subtilis, garlic extract and clove extract. The experiments were carried out through two vegetative cycles: 2015/2016 and 2016/2017. The length of wood discoloration, pathogen re-isolation percentage, fresh mass of the pruning material, peroxidase activity, fluorescence, chlorophyll index, phenological stages, shoot length and leaf area were evaluated. The treatments with clove, garlic extract, tebuconazole, pyraclostrobin, mancozeb, fosetyl-Al and B. subtilis reduced mycelial growth by more than 90%. In the grapevines, the use of T. harzianum decreased the re-isolation of L. theobromae, but no differences were verified for the other plant evaluations. We concluded that the use of T. harzianum would be a potential option for wound protection without altering the physiological aspects of cv. Syrah grapevines.

https://doi.org/10.4025/actasciagron.v43i1.44785 1038 downloads

15.

Management of white mold of soybean using winter cereal straw
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Feller, Leandro André

; Corrêa, Jessica Vanessa Wosniak

; Leite, Carla Daiane

; Santos, Leandro Alvarenga

; Faria, Cacilda Márcia Duarte Rios

; Mendes, Marcelo Cruz

Ciência Rural

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2021, Volume 51 Nº 2 elocation e20190476

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O manejo do mofo branco (Sclerotinia sclerotiorum) tem sido um dos principais limitantes de produção enfrentados por produtores de soja. Tendo em vista a complexidade do manejo desta doença e da estrutura de resistência do patógeno, o presente trabalho foi conduzido nos municípios de Guarapuava e Palmas - Paraná visando o manejo do mofo branco na cultura da soja utilizando palhadas de cereais de inverno, aveia, centeio e triticale. Inicialmente, cultivou-se os diferentes cereais de inverno, simultaneamente, nas duas áreas de estudo (Guarapuava e Palmas - PR). Avaliou-se a produção de palhada, a altura de plantas, a massa verde e seca da parte aérea. Posteriormente, semeou-se soja ‘BMX Apolo’ sobre as palhadas de cereais e avaliou-se a incidência e a severidade do mofo branco. No experimento in vitro, adicionou-se 20 escleródios cobertos por uma camada de palhas (aveia, centeio e triticale) e após 98 dias, observou-se germinação carpogênica somente no tratamento testemunha. Independentemente do local de estudo, o centeio destacou-se com maiores altura e massa verde. O cultivo sobre palhada de cereais de inverno reduziu a incidência e severidade de mofo branco com destaque para a palhada de centeio, reduzindo a incidência da doença em 77,7% e 76,6%, em Palmas e Guarapuava, respectivamente.

ABSTRACT: The management of white mold (Sclerotinia sclerotiorum(Lib.) De Bary) has been one of the main production limitations faced by soybean (Glycine max L.) producers. Considering the complex management of this disease and resistance structure of the pathogen, the present study was conducted in the municipalities of Guarapuava and Palmas in Paraná with the objective of managing the white mold of soybean using straws of winter cereals, such as oat, rye, and triticale. Initially, the three winter cereals were cultivated simultaneously in both the study areas. Straw production, plant height, and shoot fresh and dry weight were evaluated. Subsequently, BMX Apollo soybean was cultivated on cereal straws, and the incidence and severity of white mold were evaluated. In the in vitro experiment, 20 sclerotia covered by a layer of cereal (oat, rye, and triticale) straws were added and carpogenic germination was observed only in the control treatment after 98 days. Regardless of the study site, rye presented greater height and fresh weight than the remaining two cereals. Soybean cultivation on winter cereal straw reduced the incidence and severity of white mold. Cultivation on rye straw reduced mold incidence by 77.7% and 76.6% in Palmas and Guarapuava, respectively.

https://doi.org/10.1590/0103-8478cr20190476 893 downloads

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