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Screener, a board game supplemented with online resources, was introduced and distributed by the Brazilian Society of Pharmacology and Experimental Therapeutics to postgraduate programs as an instructional tool for the process of drug discovery and development (DDD). In this study, we provided a comprehensive analysis of five critical aspects for evaluating the quality of educational games, namely: 1) description of the intervention; 2) underlying pedagogical theory; 3) identification of local educational gaps; 4) impact on diverse stakeholders; and 5) elucidation of iterative quality enhancement processes. We also present qualitative and quantitative assessments of the effectiveness of this game in 11 postgraduate courses. We employed the MEEGA+ online survey, comprising thirty-three close-ended unipolar items with 5-point Likert-type response scales, to assess student perceptions of the quality and utility of Screener. Based on 115 responses, the results indicated a highly positive outlook among students. In addition, we performed a preliminary evaluation of learning outcomes in two courses involving 28 students. Pre- and post-quizzes were applied, each consisting of 20 True/False questions directly aligned with the game's content. The analysis revealed significant improvement in students' performance following engagement with the game, with scores rising from 8.4 to 13.3 (P<0.0001, paired t-test) and 9.7 to 12.7 (P<0.0001, paired t-test). These findings underscore the utility of Screener as an enjoyable and effective tool for facilitating a positive learning experience in the DDD process. Notably, the game can also reduce the educational disparities across different regions of our continental country.

https://doi.org/10.1590/1414-431x2023e13258

Exogenous amylase increases gas production and improves in vitro ruminal digestion kinetics of sorghum and corn grains
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Silva, A.S.

; Pereira, L.G.R.

; Pedreira, M.S.

; Machado, F.S.

; Campos, M.M.

; Cortinhas, C.S.

; Acedo, T.S.

; Santos, R.D.

; Rodrigues, J.P.P

; Maurício, R.M.

; Tomich, T.R.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Jun 2023, Volume 75 Nº 3 Páginas 511 - 518

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT The aim of this study was to evaluate the effect of exogenous amylase on gas production, in vitro dry matter digestibility (IVDMD), and in vitro digestion kinetics of sorghum (Sorghum vulgaris) and two corn hybrids of different grain textures. Ruminal fluid was collected from two rumen-fistulated cows receiving or not exogenous amylase (0.7g kg-1 of dry matter (DM basis)), provided to achieve 396 kilo Novo units kg-1 for amylase activity (DM basis). Gas production was measured after 1, 3, 6, 9, 12, 15, 18, 21, 24, 30, 36, 42 e 48 hours of incubation. Amylase increased gas production (mL) by 5.4%. Corn hybrids have higher in vitro dry matter digestibility than sorghum. Exogenous amylase increased the potential of gas production (A) (P=0.01). There was an effect of hybrid for IVDMD (P<0.01). The addition of exogenous amylase increases the in vitro gas production, improves fermentation kinetics, and increases the production of the ammonia nitrogen of corn and sorghum grains, but does not affect in vitro and dry matter digestibility or the short-chain fatty acids production.

RESUMO O objetivo deste estudo foi avaliar o efeito da amilase exógena na produção de gases, a digestibilidade in vitro da matéria seca (DIVMS) e a cinética de digestão in vitro de sorgo (Sorghum vulgaris) e de dois híbridos de milho de diferentes texturas de grãos. O líquido ruminal foi coletado de duas vacas fistuladas no rúmen recebendo ou não amilase exógena (0,7g kg-1 de matéria seca (MS)), fornecida para atingir 396 kg Novo unidades kg-1 para atividade de amilase (base na MS). A produção de gás foi medida após uma, três, seis, nove, 12, 15, 18, 21, 24, 30, 36, 42 e 48 horas de incubação. A amilase aumentou a produção de gás (mL) em 5,4%. Híbridos de milho apresentam maior DIVMS que o sorgo. A amilase exógena aumentou o potencial de produção de gás (A) (P=0,01). Houve efeito de híbrido para DIVMS (P<0,01). Amilase exógena aumenta a produção de gás in vitro, melhora a cinética da fermentação e aumenta a produção de nitrogênio amoniacal de grãos de milho e sorgo, mas não afeta a digestibilidade in vitro da matéria seca ou a produção de ácidos graxos de cadeia curta.

https://doi.org/10.1590/1678-4162-12803

Air velocity spatial variability in open Compost-Bedded Pack Barn system with positive pressure ventilation
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OLIVEIRA, CARLOS EDUARDO A.

; TINÔCO, ILDA DE FÁTIMA F.

; DAMASCENO, FLÁVIO A.

; OLIVEIRA, VICTOR C. DE

; RODRIGUES, PEDRO HENRIQUE M.

; FERRAZ, GABRIEL A.S.

; SOUSA, FERNANDA C. DE

; ANDRADE, RAFAELLA R.

; NASCIMENTO, JOÃO ANTÔNIO C. DO

; SILVA, LEONARDO F. DA

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220415

Resumo: En | Texto: En | PDF: En

Abstract In Compost-Bedded Pack Barn (CBP) systems, air velocity is linked with the thermal comfort of housed dairy cattle and bedding quality and, therefore, assessing ventilation efficiency is essential. In this context, the objective of this study was to evaluate and characterize dependence and spatial distribution of air velocity at the 1.5 m height (vair,M) and at bedding level (vair,B) in an open CBP system with positive pressure ventilation. The study was conducted in 2021, in a facility located in the Zona da Mata region, Minas Gerais, Brazil. The facility area was divided into a mesh composed of 55 equidistant points, where vair,M and vair,B data were collected in the morning (09:00 a.m.) and afternoon (03:00 p.m.) periods, during three weeks in Brazilian winter. Geostatistics techniques were used to assess dependence and spatial distribution. In both periods evaluated, there were a strong occurrence of spatial dependence and non-uniform vair,M and vair,B distributions. The vair,M and vair,B values were lower than recommended (1.8 m∙s–1) in more than 65.0% of the area. Adequate ventilation levels were observed only in the first 20.0 m of the facility, from Southeast to Northwest, because of the fan lines present.

https://doi.org/10.1590/0001-3765202320220415

A model for preservation of thymocyte-depleted thymus
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Dias, A.S.

; Damaceno-Rodrigues, N.R.

; Gimenez, T.M.

; Oliveira, P.M.

; Zerbini, M.C.

; Carneiro-Sampaio, M.

; Odone Filho, V.

; Jatene, M.B.

; Vasconcelos, D.M.

; Rocha, V.

; Novak, E.M.

Brazilian Journal of Medical and Biological Research

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2023, Volume 56 elocation e12647

Resumo: En | Texto: En | PDF: En

DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reconstitute T cell immunity. Thymus transplantation is a promising investigational procedure for reconstitution of thymic function in infants with congenital athymia. Here, we demonstrate a possible optimization of the preparation of thymus slices for transplantation through prior depletion of thymocytes and leukocyte cell lineages followed by cryopreservation with cryoprotective media (5% dextran FP 40, 5% Me2SO, and 5% FBS) while preserving tissue architecture. Thymus fragments were stored in liquid nitrogen at -196°C for 30 days or one year. The tissue architecture of the fragments was preserved, including the distinction between medullary thymic epithelial cells (TECs), cortical TECs, and Hassall bodies. Moreover, depleted thymus fragments cryopreserved for one year were recolonized by intrathymic injections of 3×106 thymocytes per mL, demonstrating the capability of these fragments to support T cell development. Thus, this technique opens up the possibility of freezing and storing large volumes of thymus tissue for immediate transplantation into patients with DiGeorge syndrome or atypical (Omenn-like) phenotype.

https://doi.org/10.1590/1414-431x2023e12647

Chronic progressive lymphoedema in a Friesian horse: clinical aspects and diagnostic approach - case report
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Alonso, J.M.

; Borges, A.S.

; Stievani, F.C.

; Capela, L.R.

; Rambo, A.L.A.

; Watanabe, M.J.

; Alves, A.L.G.

; Rodrigues, C.A.

; Hussni, C.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Ago 2022, Volume 74 Nº 4 Páginas 693 - 700

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O linfedema crônico progressivo (LCP) é uma enfermidade incapacitante de cavalos de tração que afeta o sistema linfático e se manifesta especialmente na região distal dos membros. A progressão da doença resulta em deformações na região distal dos membros, especialmente na quartela, com fibrose e lesões nodulares na pele. Foi atendido um garanhão da raça Friesian, de 15 anos de idade, com histórico de aumento de volume, nodulações e deformações na região distal dos membros torácicos, com início há aproximadamente quatro anos. Ao exame radiográfico simples, foram identificadas nodulações na pele e no subcutâneo e, ao exame venoso contrastado, identificou-se marcante dilatação II veia digital palmar comum em ambos os membros torácicos. Foi isolado Streptococcus equi e Proteus mirabilis ao cultivo tecidual e identificou-se sarna do tipo corióptica ao raspado de pele. No exame histopatológico, foram identificadas perivasculite e dilatação de vasos linfáticos. Os achados clínicos, histórico, de raça do animal e os exames complementares direcionaram o diagnóstico para o LCP. A pesquisa da literatura nacional não revelou descrições clínicas dessa enfermidade, mas, devido ao aumento do número de animais de tração no país nos últimos anos, a descrição dessa enfermidade se torna importante. O diagnóstico correto e precoce é necessário para retardar a progressão da doença. Nesse sentido, ressalta-se a relevância da divulgação nacional desses achados, a fim de contribuir para o diagnóstico precoce e permitir o retardo da evolução da doença.

ABSTRACT Chronic Progressive Lymphoedema (CPL) is a disabling disease of draft horses that affects lymphatic system function typically in the distal limbs. Deformities of distal limbs, especially of the pastern, with fibrosis and skin nodular lesions are consequences of disease progression. A 15-year-old Friesian stallion presented for evaluation with history of forelimb enlargement and nodule formations distal to the carpus for four years. Simple radiographs showed soft tissue nodular lesions and venous contrasted radiography showed intense enlargement of II common digital palmar vein of both forelimbs. Tissue culture tests revealed Streptococcus equi and Proteus mirabilis isolation and skin scraping test identified Chorioptes bovis mites. Histological examination revealed perivasculitis and lymph vessels distention. History, clinical and histological findings, and complementary exams suggested CPL diagnosis. We were unable to find previously published cases describing this disease in Brazil, where the increasing number of draft horses requires attention to this problem. The correct and early diagnosis substantially delays disease progression. Therefore, we highlight the need for nationwide propagation of these data to ensure better diagnosis and early treatment of future CPL cases.

https://doi.org/10.1590/1678-4162-12688

AMPA and angiotensin type 1 receptors are necessary for hemorrhage-induced vasopressin secretion
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Dos-Santos, R.C.

; Vilhena-Franco, T.

; Reis, L.C.

; Elias, L.L.K.

; Antunes-Rodrigues, J.

; Mecawi, A.S.

Brazilian Journal of Medical and Biological Research

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2022, Volume 55 elocation e11635

Resumo: En | Texto: En | PDF: En

Hypovolemia induced by hemorrhage is a common clinical complication, which stimulates vasopressin (AVP) secretion by the neurohypophysis in order to retain body water and maintain blood pressure. To evaluate the role of brain L-glutamate and angiotensin II on AVP secretion induced by hypovolemia we induced hemorrhage (∼25% of blood volume) after intracerebroventricular (icv) administration of AP5, NBQX, or losartan, which are NMDA, AMPA, and AT1 receptor antagonists, respectively. Hemorrhage significantly increased plasma AVP levels in all groups. The icv injection of AP5 did not change AVP secretion in response to hemorrhage. Conversely, icv administration of both NBQX and losartan significantly decreased plasma AVP levels after hemorrhage. Therefore, the blockade of AMPA and AT1 receptors impaired AVP secretion in response to hemorrhage, suggesting that L-glutamate and angiotensin II acted in these receptors to increase AVP secretion in response to hemorrhage-induced hypovolemia.

https://doi.org/10.1590/1414-431x2021e11635

Natural and artificial pollination of white-fleshed pitaya
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MOREIRA, RENATA A.

; RODRIGUES, MARIANE A.

; SOUZA, RAFAELA C. DE

; SILVA, ALEXANDRE D. DA

; SILVA, FÁBIO O.R.

; LIMA, CAROLINA G.

; PIO, LEILA A.S.

; PASQUAL, MOACIR

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20211200

Resumo: En | Texto: En | PDF: En

Abstract To produce pitaya (Hylocereus spp.), it is essential that pollination occur, either artificially or by pollinating agents. This study evaluated the viability of self-pollination, cross-pollination, and natural pollination, as well as pollen viability, stigma receptivity and ideal pollination window in pitaya flowers (Hylocereus spp.). An experiment was conducted with a randomized block design consisting of four treatments: T1: manual self-pollination; T2: nocturnal open pollination; T3: diurnal open pollination; and T4: manual cross-pollination - red-fleshed pitaya [ Hylocereus polyrhizus] pollen placed on white-fleshed pitaya [ Hylocereus undatus] stigma. The experiment had four replicates, with two plants per replicate (two flowers per plant), totaling 16 flowers per treatment. The analyzed variables were fruit weight, length, diameter, titratable acidity, pH, soluble solids, and SS/TA ratio; the germination percentage of pollen grains; and the receptivity of stigmas. Artificial pollination (self- and cross-pollination) of white-fleshed pitaya (Hylocereus undatus) is viable, resulting in larger fruits than natural pollination, with cross-pollination being the most recommended. Throughout the floral opening period, the pitaya flower (Hylocereus spp.) has receptive stigma capable of receiving pollen. The flowers exhibit the highest pollen germination rate at 7 p.m, which is the ideal pollination window for pitaya.

https://doi.org/10.1590/0001-3765202220211200

Effect of chromium yeast supplementation on lipid profile of swine fat
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ALENCAR, STEPHAN A.S.

; KIEFER, CHARLES

; NASCIMENTO, KARINA M.R.S

; VIANA, LUIZ HENRIQUE

; CORASSA, ANDERSON

; RODRIGUES, GABRIELA P.

; SILVA, CAMILLA M.

; CAVALHEIRO, LEANDRO F.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 Nº 3 elocation e20190619

Resumo: En | Texto: En | PDF: En

Abstract This study was conducted to evaluate the period of chromium yeast supplementation on lipid profile of backfat and Longissimus lumborum muscle of barrows. It was evaluated carcass samples from forty barrows, genetically similar. Pigs diets were supplemented with 0.4 mg kg-1 of chromium yeast in four periods (0, 38, 62 and 94 days before slaughter). The experimental design was completely randomized with four treatments, ten replicates, and each experimental unit consisting of one animal. Lipid profiles of backfat and Longissimus lumborum muscle were analyzed by gas chromatography. The increase in the period of chromium yeast use had a quadratic effect (P<0.05) for stearic and oleic fatty acids, and total saturated, monounsaturated and unsaturated fatty acids in backfat. DH-γ-linolenic and arachidonic fatty acids reduced when the period of chromium yeast use increased. In the meat, there was a quadratic effect (P<0.05) only in the γ-linoleic fatty acid. The use of chromium yeast for different periods influences the lipid profile of the backfat and the Longissimus lumborum muscle, with less effect in the meat.

https://doi.org/10.1590/0001-3765202120190619 121 downloads

Fatty acid profiles of the plasma and follicular fluid mares fed a combination of linseed and salmon oil
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VIAFARA, JESÚS A.S.

; PORTILHO, RICARDO V.

; MACULAN, RENATA

; SOUZA JÚNIOR, JOSÉ F. DE

; SILVA, CAMILLA M.

; RODRIGUES, PAULA G.

; EL AZZI, MARCELO S.

; SOUZA, JOSÉ C. DE

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20190443

Resumo: En | Texto: En | PDF: En

Abstract This study evaluated the presence of polyunsaturated fatty acids in circulating blood and in the ovarian follicular fluid of mares, after supplementation of the diet with linseed oil. Six Mangalarga Marchador mares, weighing 397.00±31.89 kg, were kept on native pasture, and assigned to the current study. In a switch over design, mares were randomly allocated to receive 150 ml of vegetable oil daily, containing polyunsaturated fatty acids n3 (62.23 g ALA, 20.34 g LA, 2.27 g EPA, 2.32 g DHA), (n=3) or no supplementation (n=3) in two replicates. Blood and follicular fluid samples were taken on the first day (D0) and every 30 days until the end of the supplementation period (D60). After 60 days of supplementation, mares were switched across the treatments. Plasma concentrations of linolenic acid in total fatty acids were higher (P=0.006) in the supplemented compared to the control group (1.89±0.13 vs. 1.49±0.13%). There were positive correlations between plasma linoleic acid and follicular fluid arachidonic acid (P=0.0106; r2=0.13) and between plasma alpha linolenic acid and follicular fluid EPA (P=0.0004; r2=0.2544). Data indicated a low to moderate relationship between the dietary linseed-based oil supplementation studied and circulating and follicular fluid polyunsaturated fatty acids contents in mares.

https://doi.org/10.1590/0001-3765202120190443 129 downloads

10.

Bases para um programa de controle da artrite encefalite caprina em rebanho leiteiro
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Alves, R.P.A.

; Rodrigues, A.S.

; Santos, V.W.S.

; Damasceno, E.M.

; Prado, G.M.

; Souza, K.C.

; Nunes Neto, T.B.

; Pinheiro, A.A.

; Cruz, M.S.P.

; Pinheiro, R.R.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Dez 2020, Volume 72 Nº 6 Páginas 2053 - 2058

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se avaliar um programa de controle da artrite encefalite caprina (AEC), por meio de testes diagnósticos sensíveis, separação de mãe e cria após o parto e medidas de manejo, com o intuito de formar rebanho livre do vírus. Utilizou-se um total de 47 cabritos da raça Saanen, mantidos isoladamente até o resultado dos primeiros testes de reação em cadeia de polimerase nested (PCR nested) e Western Blotting (WB), com base na coleta de sangue no momento do nascimento (M0). No PCR nested, quatro animais foram positivos, no M0, e foram eutanasiados. Posteriormente, os demais 43 cabritos foram submetidos à coleta de sangue aos 60 (M60) e 270 (M270) dias de vida para realização de novos testes de WB e PCR nested, que não detectaram animais positivos. Pode-se afirmar que a metodologia adotada neste estudo foi efetiva no controle da doença, nas fases de aleitamento e pós-aleitamento, e que a combinação do sistema de manejo, a fim de propiciar diminuição de risco de transmissão horizontal, com técnicas de diagnóstico mais apuradas, como o WB e a PCR nested, é relevante para elaboração de plano estratégico de controle da enfermidade.

ABSTRACT We aimed to evaluate a program to control Caprine Arthritis Encephalitis (CAE), using diagnostic tests, separation of the mother and postpartum and other management measures, in order to form a free flock of the virus. We used a total of 47 Saanengoats in isolation until the results of the first nested Polymerase Chain Reaction (nested PCR) and Western Blotting (WB) tests, based on blood collection at the time of birth (M0). In the nested PCR, 4 animals were positive, at M0, and were eliminated. Later, the other 43goats were submitted to blood collection at 60 (M60) and 270 (M270) days of life to perform new tests of WB and nested PCR, which did not detect positive animals. We can affirm that the methodology adopted in this study was effective in the control of the disease, in the phase of breastfeeding and post-breastfeeding, and that the combination of the management system, which allows a reduction of risk of horizontal transmission, with more accurate diagnostic techniques, such as WB and nested PCR, is relevant for the elaboration of a strategic plan for the disease control.

https://doi.org/10.1590/1678-4162-11695 639 downloads

11.

Effects of diflubenzuron on associated insect fauna with Anopheles(Diptera: Culicidae) in laboratory, partial-field, and field conditions in the Central Amazon
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FERREIRA, FRANCISCO A.S.

; ARCOS, ADRIANO N.

; MAIA, NATALIELLI S.G.

; SAMPAIO, RAQUEL T.M.

; COSTA, FÁBIO M.

; RODRIGUES, ILÉA B.

; TADEI, WANDERLI P.

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2020, Volume 92 Nº 1 elocation e20180590

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to analyze the possible effects of diflubenzuron on the associated insect fauna under laboratory, semi-field and field conditions. Laboratory bioassays were performed in aquariums with mortality readings (%) every 24h until 96h, and in semi-fields, insects were kept in basins with readings every 24h for up to 12 days, in triplicates and a control. In the field experiment, a collection was performed before the application of diflubenzuron in ten brick factory pits (25m²) and 15 post-application. The values of LC50 and LC90 for Chironomus were 2.77x10-3g/L and 0.86g/L, respectively, and for Buenoa, they were 0.019g/L and 0.92g/L, a strong relationship was observed between mortality and exposure time (r²>0.8) in all concentrations used. In semi-field, similar mean values of emergency inhibition were observed, except for Euthyplociidae, which was more sensitive. There was no significant difference between the data of richness and diversity of aquatic insects in the field experiment, considering an interval of 15 days (p=0.32). Finally, the data suggest that diflubenzuron may have a negative effect on the associated insect fauna, but in the field experiment the environmental conditions of Anopheles breeding sites may have affected its efficiency.

https://doi.org/10.1590/0001-3765202020180590 864 downloads

12.

Structural reorganization of CuO/Cu2[Fe(CN)6] nanocomposite: characterization and electrocatalytic effect for the hydrogen peroxide reduction
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RODRIGUES, WALLONILSON V.

; NASCIMENTO, STEFFANE Q.

; SILVA, WESLEY Y.S.

; QUINZEIRO, SANOELLE F.L.

; LUZ, ROBERTO A.S.

; CANTANHÊDE, WELTER

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2020, Volume 92 Nº 3 elocation e20191442

Resumo: En | Texto: En | PDF: En

Abstract We report the study on the formation of the Cu2[Fe(CN)6] nanocomposite, which was obtained from copper oxide nanoparticles (CuO NPs) and Prussian Blue precursors. UV-vis analysis indicated that Cu2+ ions are released from CuO NPs, while Fe3+ ions are adsorbed onto the structure of CuO due to a sharp increase in zeta potential (from -30 to 0 mV) after the formation of the Cu2[Fe(CN)6]. Moreover, energy dispersive spectroscopy confirmed that Fe3+ ions are trapped in the CuO NPs structure. The CuO/Cu2[Fe(CN)6] nanocomposite exhibited the monoclinic and face-centered cubic phases that correspond to the CuO and Cu2[Fe(CN)6] components. Cyclic voltammetry (CV) for the Nanocomposite modified electrode revealed two well-defined redox couples at -0.073 ((E1/2)1) and 0.665 mV ((E1/2)2), attributed to the conversion of Cu2+ to Cu+ and CuFe2+ CuFe3+ pairs, respectively, which is similar to those in the CuO and Cu2[Fe(CN)6]components. Furthermore, the catalytic activity of the nanocomposite towards hydrogen was investigated through CV, where the reduction of H2O2 led to increased currents for the electrochemical process associated with the first redox pair. In contrast, for isolated materials (CuO NPs and Cu2[Fe(CN)6]), there was no significant increase in the current associated with either redox pair.

https://doi.org/10.1590/0001-3765202020191442 1649 downloads

13.

Polypropylene meshes coated with chitosan/polyethylene glycol for the reconstruction of the abdominal wall: an experimental study in rats
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Rodrigues, D.F.

; Mendes, F.F.

; Melo, R.M.

; Menezes, L.B.

; Guimarães, L.L.B.

; Silva, A.S.

; Rammazzina Filho, W.A.

; Moraes, A.M.

; Paulo, N.M.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Ago 2019, Volume 71 Nº 4 Páginas 1198 - 1206

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi caracterizar as reações tissulares desencadeadas pela tela de polipropileno revestida com o filme de quitosana e polietilenoglicol e verificar se ela é capaz de prevenir a formação de aderências peritoneais. Um defeito na parede abdominal dos ratos foi realizado, e as telas de polipropileno revestidas com quitosana/polietilenoglicol (grupo CPEG, n= 12) e sem revestimento (grupo controle PP, n= 12) foram implantadas. No quarto e no 45º dia pós-operatório, avaliou-se a formação de aderências e a reação tecidual ao biomaterial por análise histológica e histoquímica. A área (P= 0,01) e a severidade (P= 0,002) da aderência peritoneal foram significativamente menores no grupo CPEG no 45º dia. No quarto dia, observou-se que a reação do corpo estranho foi menor no grupo CPEG (P= 0,018), e a produção de fibras de colágeno mais intensa (P= 0,041). As reações tissulares causadas pelos biomateriais implantados foram semelhantes no 45º dia, com exceção da melhor organização das fibras colágenas no grupo CPEG. As telas CPEG não impediram completamente a formação de aderências, porém minimizaram a gravidade do processo. A reação de corpo estranho promovida por telas de polipropileno revestidas com CPEG é menos intensa do que a desencadeada por telas de polipropileno não revestidas.

ABSTRACT The aim of this study was to characterize the tissue reactions triggered by the polypropylene mesh coated with chitosan and polyethylene glycol film, and if it’s able to prevent the formation of peritoneal adhesions. Defects in the abdominal wall of rats were induced and polypropylene meshes coated with chitosan/polyethylene glycol (CPEG group, n= 12) and uncoated (PP control group, n= 12) were implanted. On the fourth and forty-fifth postoperative day the formation of adhesion and the tissue reaction to the biomaterial was evaluated through histological and histochemical analysis. The area (P= 0.01) and severity (P= 0.002) of the adhesion was significatively less in the CPEG group. On the fourth day the foreign body reaction was less intense in CPEG group (P= 0.018) and the production of collagen fibers was more intense in this group (P= 0.041). The tissue reactions caused by the biomaterials were similar on the 45th day, with the exception of the high organization of collagen fibers in the CPEG group. The CPEG meshes did not fully prevent the formation of adhesions, but minimized the severity of the process. The foreign body reaction promoted by polypropylene meshes coated with CPEG is less intense than that triggered by uncoated polypropylene meshes.

https://doi.org/10.1590/1678-4162-9639 882 downloads

14.

Avaliação de um controle estratégico da artrite encefalite caprina em rebanho caprino leiteiro
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Rodrigues, A.S.

; Pinheiro, R.R.

; Brito, R.L.L.

; Andrioli, A.

; Oliveira, E.L.

; Sider, L.H.

; Santos, V.W.

; Oliveira, L.S.

; Dias, R.P.

; Gouveia, A.M.G.

; Teixeira, M.F.S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Métricas do periódico
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Jan 2018, Volume 70 Nº 1 Páginas 139 - 146

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste trabalho foi avaliar a utilização periódica de testes de diagnóstico mais sensíveis aliados às práticas de manejo, visando ao controle eficaz da artrite encefalite caprina (CAE). Foram realizadas oito coletas de sangue em matrizes e reprodutores. Da primeira à sétima análise, as coletas foram quadrimestrais, utilizando-se os testes de imunodifusão em gel de agarose (IDGA), ensaio imunoenzimático indireto (ELISA-i) e Western Blot (WB). A oitava coleta aconteceu seis meses após a sétima, utilizando-se o WB e a reação em cadeia de polimerase (PCR). A prevalência da CAE foi de 6,8%, 14,9% e 39,2% no IDGA, ELISA-i e WB, respectivamente. Na última análise, foram detectados 0,9% de animais positivos pelo WB e 10,8% pela PCR. Apesar de não erradicarem a CAE, as medidas adotadas, aliadas à utilização periódica dos testes sorológicos e à combinação com a PCR, foram importantes para reduzir significativamente os animais soropositivos no rebanho.

ABSTRACT The aim of this study was to evaluate the periodic use of more sensitive diagnostic tests associated to management practices for the effective control of caprine arthritis-encephalitis (CAE). We carried out eight blood samples in does and bucks. From the first to the seventh analysis, the samples were quarterly, using Agarose Gel Immunodiffusion (AGID), Enzyme linked immunosorbent assay (i-ELISA) and Western Blot (WB) tests. The eighth collection was made six months after the seventh, using the WB and Polymerase Chain Reaction (PCR). The prevalence of CAE was 6.8%, 14.9% and 39.2% in the AGID, i-ELISA and WB respectively. The last analysis detected 0.9% of animals positive by WB and 10.8% by PCR. Although they do not eradicate CAE, steps taken together with the periodic use of serological tests and the combination with PCR were important to significantly reduce positive animals in the herd.

https://doi.org/10.1590/1678-4162-9537 1496 downloads

15.

Effects of (-)-6,6'-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
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Magalhães, Lizandra G.

; Lima, Thais C.

; Paula, Renato G. de

; Morais, Enyara R.

; Aguiar, Daniela P.

; Gardinassi, Luiz G.

; Garcia, Gustavo R.

; Laurentiz, Rosangela S.

; Rodrigues, Vanderlei

; Bastos, Jairo K.

; Filho, Ademar A.S.

; Yatsuda, Ana P.

; Cunha, Wilson R.

; Silva, Márcio L.A.

Revista Brasileira de Farmacognosia

Métricas do periódico
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Jun 2016, Volume 26 Nº 3 Páginas 334 - 341

Resumo: En | Texto: En | PDF: En

Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (-)-6,6'-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies.

https://doi.org/10.1016/j.bjp.2016.02.001 1231 downloads

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