The publication Cardiovascular Statistics – Brazil aims to provide an annual compilation of data and research on the epidemiology of CVDs in Brazil.  The report integrates official statistics from the Brazilian Ministry of Health and other governmental entities alongside data from the GBD project, coordinated by the IHME at the University of Washington. Additionally, it incorporates data derived from various sources and scientific studies, including cohorts and registries, that relate to CVDs and their associated risk factors. This publication is intended for a wide range of individuals, including researchers, clinicians, patients, healthcare policymakers, media professionals, the general public, and other interested parties seeking extensive national data about heart disease and stroke.  Volunteer researchers from various Brazilian universities and research institutions carry out the project. The group is led by a five-member steering committee (ALPR, CAP, DCM, GMMO, and LCCB). The Brazilian Society of Cardiology fully supports this initiative, and the project receives collaborative support from the GBD Brazil Network and an International Committee (GAR, PP, and TAG) from both the IHME/University of Washington (GAR) and the World Heart Federation (PP and TAG). 

A publicação Estatística Cardiovascular – Brasil tem por objetivo fornecer uma compilação anual dos dados e das pesquisas sobre a epidemiologia das DCV no Brasil.  Este documento integra as estatísticas oficiais do Ministério da Saúde do Brasil e outras entidades governamentais ao lado de dados do projeto GBD, coordenado pelo IHME da Universidade de Washington. Além disso, incorpora dados derivados de várias fontes e estudos científicos, inclusive coortes e registros, relacionados às DCV e fatores de risco associados. Esta publicação destina-se a um público variado, incluindo pesquisadores, clínicos, pacientes, formuladores de políticas de saúde, profissionais da mídia, o público em geral e todos aqueles que buscam dados nacionais abrangentes sobre DCV e acidente vascular cerebral. Pesquisadores voluntários de várias universidades e instituições de pesquisa brasileiros realizaram este projeto. O grupo é liderado por um comitê diretivo com cinco membros (ALPR, CAP, DCM, GMMO e LCCB). A Sociedade Brasileira de Cardiologia apoia integralmente esta iniciativa e o projeto recebe colaboração da Rede GBD Brasil1 e do GBD International Committee (GAR, PP e TAG) do IHME/Universidade de Washington (GAR) e da World Heart Federation (PP e TAG). 

Abstract Background: The transition to higher education is a period of change during which students have new experiences that influence their lifestyles. Objective: To identify the domains of higher education students’ lifestyles that require intervention. Methodology: This cross-sectional descriptive correlational study was conducted with 522 higher education students. The Fantastic Lifestyle Assessment questionnaire was used, and statistical analysis was conducted using the IBM SPSS Statistics software - version 25.0. Results: The study participants had a median age of 22, and 78.4% were women. Of the 522 students in the sample, 17.8% were overweight and 6.3% were obese, 14.4% were diagnosed with a chronic disease, 35.6% had Very Good lifestyles, and 20.1% had Good lifestyles. The domains Health and sexual behavior (x-= 8.97/24) and Work/type of personality (x-= 6.82/12) obtained negative scores. Significant differences were found in the questionnaire’s total score (U =19785.00; p < 0.05) when considering the variables Gender, with female students having better lifestyles (x-= 89.16; SD = 12.75) and Course year (H = 18.80; p < 0.01). Conclusion: Assessing higher education students’ lifestyles offers the possibility to describe the intervention foci and guide the health promotion interventions.

Resumo Enquadramento: A transição para o ensino superior representa um período de mudança em que o jovem se depara com novas vivências, com implicações no seu estilo de vida. Objetivo: Identificar os domínios dos Estilos de vida dos estudantes do ensino superior que necessitam de intervenção. Metodologia: Estudo descritivo-correlacional, transversal, com uma amostra de 522 estudantes do ensino superior. Questionário de Estilos de Vida Fantástico. Análise estatística em IBM SPSS Statistics 25.0. Resultados: Mediana de idade 22 anos, 78,4% sexo feminino. Dos 522 estudantes, 17,8% excesso peso; 6,3 % obesidade; 14.4% portadores de doença crónica; 35,6% apresentaram Estilos de Vida Muito Bom e 20,1% Estilos de Vida Bom. Destacou-se pela negativa Comportamentos Saúde e Sexual (x-= 8,97/24), Trabalho/Tipo Personalidade (x-= 6,82/12). Há diferenças significativas no score global do questionário (U = 19785,00; p < 0,05) para estudantes as mulheres com melhor Estilo de Vida (x-= 89,16; DP = 12,75); e em função Ano do curso (H = 18,80; p < 0,01). Conclusão: A avaliação dos Estilos de Vida permitiu caraterizar os focos de intervenção e orientar intervenções de promoção da saúde.

Resumen Marco contextual: La transición a la enseñanza superior representa un periodo de cambio en el que los jóvenes se enfrentan a nuevas experiencias con implicaciones para su estilo de vida. Objetivo: Identificar los dominios de los estilos de vida de los estudiantes de enseñanza superior que necesitan intervención. Metodología: Estudio descriptivo-correlacional, transversal, muestra de 522 estudiantes de enseñanza superior. Cuestionario de estilos de vida Fantástico. Análisis estadístico en IBM SPSS Statistics 25.0. Resultados: Mediana de edad de 22 años, el 78,4% del sexo femenino. De los 522 estudiantes, el 17,8% con exceso de peso; el 6,3 % con obesidad; el 14.4% con una enfermedad crónica; el 35,6% presentó estilos de vida muy bueno, y el 20,1% estilos de vida bueno. Salud y comportamento sexual destacó negativamente (x-= 8,97/24), Trabajo/Tipo Personalidad (x-= 6,82/12). Existen diferencias significativas en la puntuación global del cuestionario (U = 19785,00; p < 0,05) para estudantes mujeres con un mejor estilo de vida (x-= 89,16; DP = 12,75) y dependiendo del año del curso (H = 18,80; p < 0,01). Conclusión: La evaluación de estilos de vida permitió caracterizar los focos de intervención y orientar las intervenciones de promoción de la salud.

Abstract Background: The identification of delirium by nurses extremely relevant. The Portuguese version of the Nursing Delirium Screening Scale (Nu-DESC) is a tool that can be used in this setting. Objective: To evaluate the psychometric properties of the Portuguese version of the Nu-DESC in a surgical context. Methodology: Methodological study with patients aged 65 years or older with a diagnosis of proximal femur fracture. Three assessment tools were used to analyze the agreement between the results. Results: In a sample of 81 patients, the sensitivity of the Nu-DESC was 92% compared to the Confusion Assessment Method (CAM) and 91% compared to the application of the DSM-5 criteria. The specificity of the instrument was 98% compared to CAM and 94% compared to the DSM-5. Cronbach’s alpha ranged between 0.86 and 0.89 Conclusion: The evaluation of the psychometric properties of the Nu-DESC in a surgical context confirmed the validity and reliability of the instrument. The results show that the scale has a high sensitivity and specificity for assessing delirium.

Resumo Enquadramento: A identificação do delirium pelos enfermeiros é de extrema relevância. Assim, parece relevante a possibilidade de recurso à versão portuguesa da Nursing Delirium Screening Scale (Nu-DESC). Objetivo: Avaliar as propriedades psicométricas da versão portuguesa da Nu-DESC em contexto cirúrgico. Metodologia: Estudo metodológico, incluindo utentes com 65 ou mais anos de idade, com o diagnóstico de fratura proximal do fémur. Recorreu-se a três medidas de avaliação para analisar a concordância entre os resultados obtidos através das mesmas. Resultados: Numa amostra de 81 utentes, a sensibilidade da Nu-DESC foi de 92% relativamente ao Confusion Assessment Method (CAM) e de 91% face à aplicação dos critérios do DSM-5. Já a especificidade do instrumento foi de 98% relativamente ao CAM e de 94% face à aplicação dos critérios do DSM-5. O alfa de Cronbach da Nu-DESC variou entre 0,86 e 0,89. Conclusão: A avaliação das propriedades psicométricas da Nu-DESC num contexto cirúrgico confirmou que a escala apresenta uma elevada sensibilidade e especificidade para a avaliação de delirium nesse mesmo contexto.

Resumen Marco contextual: La identificación del delirio por parte del personal de enfermería es extremadamente importante. De ahí la posibilidad de utilizar la versión portuguesa del Nursing Delirium Screening Scale (Nu-DESC). Objetivo: Evaluar las propiedades psicométricas de la versión portuguesa del Nu-DESC en entornos quirúrgicos. Metodología: Estudio metodológico que incluyó a usuarios de 65 años o más, con diagnóstico de fractura proximal de fémur. Se utilizaron tres medidas de evaluación para analizar la concordancia entre los resultados obtenidos a través de ellas. Resultados: En una muestra de 81 usuarios, la sensibilidad del Nu-DESC fue del 92% en relación con el Confusion Assessment Method (CAM) y del 91% cuando se aplican los criterios del DSM-5. La especificidad del instrumento fue del 98% en relación con el CAM y del 94% frente a la aplicación del DSM-5. El alfa de Cronbach de la Nu-DESC varió entre 0,86 y 0,89. Conclusión: La evaluación de las propiedades psicométricas de la Nu-DESC en un contexto quirúrgico confirmó que la escala presenta una alta sensibilidad y especificidad para la evaluación del delirio en este mismo contexto.

Abstract Background: Delirium is a common condition in institutionalized adults, characterized by disturbance of consciousness, decreased attention span and disorganized thinking, with an evolution in a short period of time. Delirium has a more detailed symptomatic presentation than acute confusion, being the first one a medical diagnosis and the second a nursing diagnosis. Acute confusion is often subject to misinterpretation by nurses. Aim: To identify autonomous nursing interventions capable of preventing acute confusion. Methods: Integrative Review, starting from the question: “Which autonomous nursing interventions can prevent acute confusion?” Research carried out in october 2020 using the following databases: CINAHL®Complete; MEDLINE Complete; Nursing & Allied Health collection: Comprehensive; Cochrane Central Register of Controlled Trials; Cochrane Database of Systematic Reviews and SciELO, with the inclusion criteria: articles with full text, between 2015 and 2020, in portuguese, spanish and english. Results: 71 results were identified and ten articles were included. The selection was made by reading the title, eliminating duplicates, reading abstracts and full texts, using Preferred Reporting Items for Systematic Reviews and Meta-Analyses, according to the inclusion criteria. The articles included are: two systematic reviews; four meta-analyses; two experimental studies; a quasi-experimental study and a literature review. Conclusions: Autonomous nursing interventions fit into the ten domains of multicomponent interventions, being perceived a heterogeneity of interventions. Multicomponent interventions are those that show better results, when compared to isolated interventions.

Resumen Introducción: El delirium es frecuente en adultos institucionalizados, caracterizado por alteración de la conciencia, disminución de la capacidad de atención, pensamiento desorganizado y con evolución en corto espacio de tiempo. El delirium tiene una presentación de síntomas más detallada que la confusión aguda, siendo el primero un diagnóstico médico y el segundo un diagnóstico de enfermería. La confusión aguda es, muchas veces, sujeta a malas interpretaciones por parte de los enfermeros. Objetivo: Identificar intervenciones autónomas de enfermería capaces de prevenir la confusión aguda. Métodos: Revisión integrativa, a partir de la pregunta: “¿Qué intervenciones autónomas de enfermería pueden prevenir la confusión aguda?”. Investigación realizada en octubre de 2020 utilizando las siguientes bases de datos: CINAHL®Complete; MEDLINE Complete; Nursing & Allied Health collection: Comprehensive; Cochrane Central Register of Controlled Trials; Cochrane Database of Systematic Reviews y SciELO, con los criterios de inclusión: artículos con texto completo, entre 2015 y 2020, en portugués, español e inglés. Resultados: Se identificaron 71 resultados, se incluyeron diez artículos. La selección se realizó mediante lectura de título, eliminación de duplicados, lectura de resúmenes y textos completos utilizando el Preferred Reporting Items for Systematic Reviews and Meta-Análisis, de acuerdo con los criterios de inclusión determinados. Los artículos incluidos son: dos revisiones sistemáticas; cuatro metanálisis; dos estudios experimentales; un estudio cuasi-experimental y una revisión de la literatura. Conclusiones: Las intervenciones autónomas de enfermería encajan en los diez dominios de intervenciones multicomponientes, dándose cuenta de una heterogeneidad de intervenciones. Las intervenciones multicomponientes son las que muestran mejores resultados, en comparación con las intervenciones aisladas.

Resumo Introdução: O delirium é frequente em adultos institucionalizados, caracterizado por perturbação da consciência, diminuição da capacidade de atenção, pensamento desorganizado e com evolução num curto espaço de tempo. O delirium tem uma apresentação sintomática mais detalhada do que a confusão aguda, sendo o primeiro um diagnóstico médico e o segundo um diagnóstico de enfermagem. A confusão aguda é muitas vezes sujeita a interpretações erróneas por parte dos enfermeiros. Objetivo: Identificar as intervenções autónomas de enfermagem passíveis de prevenir a confusão aguda. Métodos: Revisão Integrativa, partindo da questão: “Quais as intervenções autónomas de enfermagem passiveis de prevenir a confusão aguda?”. Pesquisa realizada em outubro de 2020 com recurso às bases de dados: CINAHL®Complete; MEDLINE Complete; Nursing & Allied Health collection: Comprehensive; Cochrane Central Register of Controlled Trials; Cochrane Database of Systematic Reviews e SciELO, com os critérios de inclusão: artigos com texto integral, entre 2015 e 2020, em português, espanhol e inglês. Resultados: Identificaram-se 71 resultados, dez artigos foram incluídos. A seleção foi feita por leitura do título, eliminação de duplicados, leitura de resumos e textos integrais com recurso ao Preferred Reporting Items for Systematic Reviews and Meta-Analyses, de acordo com os critérios de inclusão determinados. Os artigos incluídos são: duas revisões sistemáticas; quatro meta-análises; dois estudos experimentais; um estudo quasi-experimental e uma revisão da literatura. Conclusões: As intervenções autónomas de enfermagem enquadram-se nos dez domínios das intervenções multicomponentes, percebendo-se uma heterogeneidade de intervenções. As intervenções multicomponentes são as que melhores resultados evidenciam, quando comparadas com as intervenções isoladas.

ABSTRACT Purpose To evaluate the penile morphology after the isolated and combined administration of dutasteride and tamsulosin in a rodent model. Materials and Methods Forty male rats were assigned into the following groups: Control group (C, receiving distilled water, n=10); Dutasteride group (D, receiving 0.5 mg/Kg/day of dutasteride, n=10); Tamsulosin group (T, receiving 0.4 mg/Kg/day of tamsulosin, n=10); and Dutasteride associated with Tamsulosin group (DT, receiving both drugs n = 10). All drugs were administered via oral gavage. After 40 days, the animals were submitted to euthanasia and their penises were collected for histomorphometric analyses. Data were compared using one-way ANOVA followed by Bonferroni’s post-test, considering p<0.05 as significant. Results The sinusoidal space and smooth muscle fiber surface densities (Sv), and the cross-sectional penile areas of rats in groups D, T and DT were reduced in comparison to controls with the most notable reductions in the combined therapy group. The connective tissue and elastic system fibers Sv were augmented in groups D, T and DT in comparison with the control group, again with the most pronounced changes observed in animals receiving the combined therapy. Conclusion Both treatments with dutasteride or tamsulosin promoted penile morphometric modifications in a rodent model. The combination therapy resulted in more notable modifications. The results of this study may help to explain the erectile dysfunction observed in some men using these drugs.

Resumo Este artigo tem por intuito compreender os dilemas e as fricções nas relações disciplinares que se estabelecem em processos judiciais de demarcação de terras indígenas. Para tanto, analisar-se-á um laudo de assistência técnica elaborado no curso do processo judicial de demarcação da Terra Indígena Potrero Guaçu, ocupada por indígenas Guarani no estado de Mato Grosso do Sul. Para realizar a análise, buscou-se identificar os principais conceitos expostos no supramencionado laudo e confrontá-los com a literatura estabelecida sobre o tema. Dessa forma, procurou-se demonstrar que para um eficaz exercício da jurisdição no transcurso de lides fundiárias que envolvam populações indígenas, não basta o mero conhecimento dos dispositivos legais. Além disso, é preciso compreender quais as especificidades, saberes, metodologias e técnicas permitem que o perito antropólogo elabore um laudo cientificamente embasado, a fim de que possa ser realizada uma adequada apreciação da prova pericial nesse contexto de conflito fundiário. tanto analisarseá analisar á Guaçu Sul análise buscouse buscou confrontálos confrontá los tema forma procurouse procurou legais disso especificidades saberes embasado fundiário

Abstract This article aims to understand the dilemmas and frictions in the relationships between Anthropology and Law, which are established in traditional lawsuits about demarcation of Indigenous territories. Therefore, the technical assistance report prepared in the course of the judicial process of demarcation of the Potrero Guaçu Indigenous Territory, occupied by Guarani Indigenous people in Mato Grosso do Sul, will be analyzed. To carry out an analysis, we sought to identify the main concepts exposed in the aforementioned report, and confront them with an established literature on the subject. In this way, we sought to demonstrate which for an effective device of land disputes about Indigenous territories, knowledge about the judicial procedures is not enough. In addition, it is necessary to understand the specificities, knowledge, methodologies and techniques that allow the anthropologist expert to prepare a scientifically based report, so that an adequate assessment of the expert evidence can be carried out in this context of land conflict. Law territories Therefore Territory Sul analyzed analysis subject way enough addition specificities conflict

RESUMO: O uso contínuo de inseticidas sintéticos para o controle do mosquito vetor de arbovírus, Aedes aegypti, tem levado à seleção natural de populações resistentes a diferentes grupos químicos. Assim, compostos derivados de plantas surgiram como uma alternativa viável para o controle desses vetores. Desse modo, este estudo foi realizado para determinar se o extrato metanólico bruto (CME) das folhas de Clibadium surinamense possui atividade larvicida contra Ae. aegypti. Para isso, Larvas de terceiro e quarto instar de Ae. aegypti foram mantidas em recipientes contendo 99 mL de água e 1 mL de CME diluído em etanol nas concentrações de 250, 500, 750 e 1000 ppm. O grupo controle continha 99 mL de água e 1 mL de etanol. Três ensaios foram realizados em triplicata para cada grupo. Após 24 horas de observação, de acordo com a análise de variância de uma via,os valores de CL50 e CL90 foram de 283 e 430 ppm, respectivamente. Em conclusão, demonstramos pela primeira vez que o CME das folhas de C. surinamense apresenta atividade larvicida contra Ae. aegypti em condições de laboratório.

ABSTRACT: The continuous use of synthetic insecticides for controlling the arboviral vector Aedes aegypti has led to the natural selection of mosquito populations resistant to different chemical groups. Thus, plant-derived compounds have emerged as a viable alternative for vectorcontrol. This study determined whether the crude methanolic extract (CME) from leaves of Clibadium surinamense has larvicidal activity against Ae. aegypti. Third- and fourth-instar Ae. Aegyptilarvae were kept in recipients containing 99 mL of water and 1mL of ethanol-diluted CMEat concentrations of 250, 500, 750, and 1000 ppm. The control group contained 99 mL of water and 1 mL of ethanol. Three trials were performed in triplicate for each group.After 24 hours of treatment, the LC50 and LC90 values were determined to be 283 and 430 ppm, respectively, according to one-way analysis of variance. In conclusion, we have demonstrated for the first time that the CME from leaves of C. surinamense show larvicidal activity against Ae. aegypti under laboratory conditions.

ABSTRACT Introduction: in videolaparoscopic surgery, movements are conducted from a twenty times magnified image of an indirect operative field. The video interface used assumes the need for depth perception using two dimensions instead of three. Objective: to evaluate the effectiveness of training to perform the laparoscopic knot in a silicone model, in 8-hour courses, and to analyze the correlation of learning outcomes with factors such as: sex, age, laterality, previous knowledge in endosuture and medical specialty. Material and Methods: in this prospective and randomized study, 56 students were evaluated, who took 8-hour courses, with groups of up to ten students. We used: a white box with camera, LCD screen and silicone piece. Four exercises were performed on the silicone mold: right hand, left hand, needle at 45° and back hand. Results: 56 students (mean age = 33.28 years). The female group, n=18, mean age 29.61 years, 17 right-handed and 1 left-handed. The male group, n=38, mean age 34.57 years, 35 right-handers, 1 left-handed and 2 ambidextrous. In both groups, no correlation was observed between the analyzes of well performed knots when correlated with the age or sex of the participants. Conclusion: laparoscopic knot training, in silicone molds, in 8 h courses, proved to be effective. Factors such as gender, age, laterality, previous knowledge in endosuture and medical specialty do not interfere with the learning results.

RESUMO Introdução: na cirurgia videolaparoscópica os movimentos são conduzidos a partir de uma imagem magnificada de vinte vezes de um campo operatório indireto. A interface de vídeo utilizada pressupõe a necessidade de percepção de profundidade utilizando duas dimensões ao invés de três. Objetivo: avaliar a eficácia do treinamento de execução do nó laparoscópico em modelo de silicone, em cursos de 8h, e analisar a correlação dos resultados de aprendizagem com fatores como: sexo, idade, lateralidade, conhecimentos prévios em endosutura e especialidade médica. Material e Métodos: Neste estudo, prospectivo e randomizado, foram avaliados 56 alunos, que realizaram cursos de 8h de duração, com turmas de até dez alunos. Foram utilizados: uma caixa branca com câmera, tela de LCD e peça de silicone. No molde de silicone foram executados quatro exercícios: mão direita, mão esquerda, agulha a 45° e back hand. Resultados: 56 alunos (média de idade = 33,28 anos). O grupo feminino, n=18, média de idade 29,61 anos, 17 destras e 1 canhota. O grupo masculino, n=38, média de idade 34,57 anos, 35 destros, 1 canhoto e 2 ambidestros. Em ambos grupos não foram observados nenhuma correlação entre as análises de nós bem executados quando correlacionados com a idade ou sexo dos participantes. Conclusão: o treinamento de nó laparoscópico, em moldes de silicone, em cursos de 8h de duração, mostrou-se eficaz. Fatores como sexo, idade, lateralidade, conhecimentos prévios em endosutura e especialidade médica, não interferem nos resultados do aprendizado.

Abstract A lectin from the marine sponge Haliclona (Reniera) implexiformis (HiL) was isolated by affinity chromatography on Sepharose™ matrix. HiL showed specificity for galactose and its derivatives. The glycoproteins porcine stomach mucin (PSM) and bovine stomach mucin (BSM) were potent inhibitors. Hemagglutinating activity of the lectin was maximal between pH 5.0 and 9.0. The lectin remained active until 60°C. The presence of CaCl2 and EDTA did not affect the hemagglutinating activity. In SDS-PAGE, HiL showed a single band of 20 kDa under reduced conditions, whereas in the non-reducing conditions, it showed a band of 20 kDa and one additional band of 36 kDa. The average molecular mass determined by Electrospray Ionization Mass Spectrometry (ESI-MS) was 35.874 ± 2 Da in native and non-reducing conditions, whereas carboxyamidomethylated-lectin showed 18,111 Da. These data indicated that HiL consists in a dimer formed by identical subunits linked by disulfide bonds. Partial amino acid sequence of HiL was determined by mass spectrometry, and revealed that it is a new type of lectin, which showed no similarity with any protein. Secondary structure consisted of 6% α-helice, 31% β-sheet, 18% β-turn and 45% random coil. HiL showed significant reduction in the number of viable cells of Staphylococcus biofilms.

Abstract The diagnosis of irreversible pulpitis (IP) depends on clinical data, especially the chief complaint of the patient, visual inspection, response to the application of stimuli, and radiographic examination. The characterization of nerve fibers (NF) in IP may contribute to better interpret painful symptoms, but has been barely explored. This study sought to characterize the density and integrity of NF in 16 samples of IP and in five healthy pulps (HP) using S-100 and PGP 9.5 markers. Immunohistochemistry was performed to determine the density/mm2 of S-100+ and PGP 9.5+ in NF. The amount of degenerated NF was obtained by subtracting the total NF density from the amount of intact NF. Associations between NF density and integrity and symptomatology were calculated. All samples were positive for S-100 and PGP 9.5. Compared to HP samples (38.20/mm2), IP samples had a lower density of intact NF (6.24/mm2). A significantly higher density of degenerated NF was found in IP samples with spontaneous pain (39.59/mm2) compared to those with provoked pain (23.96/mm2) (p = 0.02). No association was observed between intensity of the inflammatory infiltrate and NF density and integrity (p > 0.05). The findings of this study suggest that pulpitis may involve different stages of degeneration and may be more advanced in cases with spontaneous pain. The symptoms reported by affected individuals do not appear to depend on the intensity of the inflammatory infiltrate, but rather on the integrity of NF.

The use of biogenic residues to obtain calcium phosphate compounds, mainly hydroxyapatite (HAp), is an excellent alternative to reduce costs in obtaining biomaterials. The purpose of this study was to obtain hydroxyapatite from fish bones and hen eggshells by alkaline hydrolysis and precipitation, respectively, and compare them to HAp obtained from calcium commercial source based on physical-chemical properties and in vitro assays. The biomaterials obtained were characterized by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) method and Fourier Transform Infrared Spectroscopy (FTIR). The XRD results revealed the presence of the main characteristic peaks of single HAp phase and FTIR analysis showed various functional groups, such as PO43-, CO3-2 and OH-, confirming the presence of HAp. SEM observations of the synthesized HAp showed a rod-like and spherical-like morphology. The cell viability and bioactivity of the materials were evaluated by rezasurin reduction and McCoy medium assays, respectively. The biomaterials obtained had no toxic effect and the sample obtained from fish bone was more bioactive when compared to the others. Therefore, the biowastes can be used as an alternative source of calcium in the synthesis of hydroxyapatite with promising properties for application in the biomedical area.

ABSTRACT. Cotton is one of the largest agricultural commodities that generate various sources of foreign exchange and employment worldwide. However, water deficiency is an environmental factor that limits the production of this crop, especially in semi-arid regions. We evaluated pyruvate supplementation to mitigate the effects of water stress on colored cotton. Experiments were conducted in a greenhouse. We studied two forms of pyruvate supplementation (SP1- via seed and foliar and SP2- only via foliar); three conditions of irrigation management of the plants: water restriction in the vegetative phase (VE), flowering (FL), and vegetative and flowering (VE/FL); and additional treatment (total irrigation throughout the crop cycle and without pyruvate supplementation). The experimental design included a randomized block in a 2 × 3 + 1 factorial scheme. The factors resulted in seven treatments with three replications, with a total of 21 experimental units. Gas exchange, enzyme activity, and production of components were evaluated. Water restriction in the vegetative phase does not cause losses in BRS Jade cotton when supplemented with pyruvate. However, in the flowering and vegetative phases plus flowering, it reduces gas exchange and production components and increases the activity of antioxidant enzymes in relation to plants under full irrigation. Supplementation with pyruvate via seed plus foliar (SP1) was better for BRS Jade cotton grown under water restriction.

Abstract A new lectin from marine sponge Ircinia strobilina, denominated IsL, was isolated by combination of affinity chromatography in Guar gum matrix followed by size exclusion chromatography. IsL was able to agglutinate native and enzymatically treated rabbit erythrocytes, being inhibited by galactosides, such as α-methyl-D-galactopyranoside, β-methyl-D-galactopyranoside and α-lactose. IsL hemagglutinating activity was stable at neutral to alkaline pH, however the lectin loses its activity at 40° C. The molecular mass determinated by mass spectrometry was 13.655 ± 5 Da. Approximately 40% of the primary structure of IsL was determined by mass spectrometry, but no similarity was observed with any protein. The secondary structure of IsL consists of 28% α-helix, 26% β-sheet, and 46% random region, as determined by dichroism circular. IsL was a calcium-dependent lectin, but no significant variations were observed by circular dichroism when IsL was incubated in presence of calcium and EDTA. IsL was not toxic against Artemia nauplii and did not have antimicrobial activity against bacterial cells. However, the IsL was able to significantly inhibit the biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis.