Eleven dairy properties from Pernambuco State in Brasil were studied; six with hand milking and five with machine milking. The herds were constituted by animals of several races, ages and lactation periods. 185 cows were studied, resulting in 708 quarters sampled. Before sampling, the California Mastitis Test (CMT) was carried out independently of this result samples were collected for microbiologic cultures and Somatic Cells Counts (SCC) and milk composition. Prevalencies of 39,3% and 54,8% (CMT); 57,2% and 63,3% (microbiologic culture); 33,4% and 49,4% (SCC) were obtained for hand and machine milking respectively. Pathogens were isolated in 61% of the samples with higher prevalence for Corynebacterium sp. (45.0%), Staphylococcus sp. (29.6%) and Streptococcus sp. (14.6%) in hand milking; Staphylococcus sp. (36.4%), Corynebacterium sp. (27.6%), Micrococcus sp. (15.6%) and Streptococcus sp. (12.9%) in machine milking. Mechanically milked herds had higher prevalence of subclinical mastitis, both for diagnosis by CMT, as for CCS and bacteriological culture with significant differences in CMT and CCS. The sensibility pattern of the pathogens indicated bigger susceptibility for the combination Neomicine-Bacitracine-Tetracicline (NBT), Florfenicol (FLF), Cefquinome (CEQ) and Enroflorxacine (ENO) in that order; Oxaciline (OXA) was the antimicrobial with smaller percent of sensibility among the pathogens.
Se estudiaron 11 propiedades productoras de leche bovina del estado de Pernambuco, Brasil; seis con ordeño manual y cinco con ordeño mecánico. Los rebaños estaban constituidos por animales de varias razas, edades y periodos de lactación. Se tomaron muestras de 185 vacas, resultando en 708 cuartos muestreados. Antes de colectar se realizó la prueba de California (CMT), con independencia de este resultado fueron colectadas muestras para cultivo microbiológico y conteo de células somáticas (CCS) y composición. Se obtuvieron prevalencias de 39,3% y 54,8% (CMT), 57,2% y 63,3% (diagnóstico microbiológico), 33,4% y 49,4% (CCS), para ordeño manual y mecánico respectivamente. Se aislaron microorganismos patógenos en el 61% de las muestras con mayor prevalencia para Corynebacterium sp. (45,0%), Staphylococcus sp. (29,6%) y Streptococcus sp. (14,6%) en ordeño manual; Staphylococcus sp. (36,4%), Corynebacterium sp. (27,6%), Micrococcus sp. (15,6%) y Streptococcus sp. (12,9%) en ordeño mecánico. Los rebaños ordeñados mecánicamente tuvieron mayor prevalencia de mastitis subclínica, tanto para el diagnóstico por CMT, como para CCS y cultivo bacteriológico; con diferencias significativas en CMT y CCS. El patrón de sensibilidad de los patógenos hallados indicó mayor susceptibilidad hacia la combinación Neomicina-Bacitracina-Tetraciclina (NBT), Florfenicol (FLF), Cefquinome (CEQ) y Enroflorxacina (ENO) en ese orden; la Oxacilina (OXA) resultó ser el antimicrobiano con menor porciento de sensibilidad entre los patógenos.