ABSTRACT BACKGROUND: Although there is a growing body of research pointing towards the need to investigate how different movement behaviors, such as physical activity and sleep, influence each other, the joint relationship between these factors and insomnia has been little explored among adolescents in developing countries. OBJECTIVES: To investigate the association between daily physical activity and insomnia in a national sample of Brazilian adolescents, according to the Human Development Index (HDI) of each Brazilian region. DESIGN AND SETTINGS: Cross-sectional study on 102,072 Brazilian students aged 11 to 19 years, selected from all regions of the country. METHODS: Information on insomnia and physical activity was self-reported by adolescents. RESULTS: Our analyses revealed that girls who accumulated at least 60 minutes/day of physical activity on up to three days/week were less prone to present insomnia. This pattern of association was maintained only for those who lived in high HDI regions (odds ratio, 0.87; 95% confidence interval, 0.75-0.99). For boys, there was a positive association between the number of active days and protection against insomnia, especially for those who lived in high HDI regions. CONCLUSION: Even amounts of physical activity that were lower than the weekly guidelines, were associated with better sleep quality for Brazilian adolescents, especially girls, and even for those who lived in regions with greater social and economic vulnerability.

Abstract Background: The resistance against antimalarial drugs represents a global challenge in the fight and control of malaria. The Brazilian biodiversity can be an important tool for research and development of new medicinal products. In this context, toxinology is a multidisciplinary approach on the development of new drugs, including the isolation, purification, and evaluation of the pharmacological activities of natural toxins. The present study aimed to evaluate the cytotoxicity, as well as the antimalarial activity in silico and in vitro of four compounds isolated from Rhinella marina venom as potential oral drug prototypes. Methods: Four compounds were challenged against 35 target proteins from P. falciparum and screened to evaluate their physicochemical properties using docking assay in Brazilian Malaria Molecular Targets (BraMMT) software and in silico assay in OCTOPUS® software. The in vitro antimalarial activity of the compounds against the 3D7 Plasmodium falciparum clones were assessed using the SYBR Green I based assay (IC50). For the cytotoxic tests, the LD50 was determined in human pulmonary fibroblast cell line using the [3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay. Results: All compounds presented a ligand-receptor interaction with ten Plasmodium falciparum-related protein targets, as well as antimalarial activity against chloroquine resistant strain (IC50 = 3.44 μM to 19.11 μM). Three of them (dehydrobufotenine, marinobufagin, and bufalin) showed adequate conditions for oral drug prototypes, with satisfactory prediction of absorption, permeability, and absence of toxicity. In the cell viability assay, only dehydrobufotenin was selective for the parasite. Conclusions: Dehydrobufotenin revealed to be a potential oral drug prototype presenting adequate antimalarial activity and absence of cytotoxicity, therefore should be subjected to further studies.

Abstract Aim: This study aimed to examine the association between physical activity (PA) indicators and TV viewing as a function of the Human Development Index (HDI). Method: This cross-sectional study was based on data from the National School Health Survey, which was composed of 102,072 students (14.28±1.03; 51.3% girls). Total PA, active commuting to school (ACS) and TV viewing were assessed by questionnaires and classified through a gradual scale ranging from “F” (low) to “A+” (high). The correlation between total PA, ACS, TV viewing and HDI was verified by Spearman’s Correlation and presented in rs. Results: HDI was positively associated with total PA [girls: rs = 0.572 (p < 0.001); boys: rs = 0.843 (p < 0.001)] and ACS [girls: rs = 0.433 (p < 0.001); boys: rs = 0.554 (p < 0.001)]; while a negative correlation was found between HDI and TV viewing [girls: rs = -0.330 (p < 0.001); boys: rs = -0.348 (p < 0.001)]. Conclusions: Brazilian adolescents from states with higher HDI were more active and spent more time watching TV than their counterparts from states with lower HDI.

Abstract Aims: 1) to identify the prevalence of active commuting to school (ACS) among Brazilian regions; and 2) to determine associated factors related to ACS in this population. Methods: Cross-sectional study comprising 16,493 adolescents (mean age 14.09±2.12 years). The data comes from the National School Health Survey (2015), and the information was collected by a self-reported questionnaire. Logistic regression models were performed to identify correlates of ACS. Results: Adolescents who live in the Southeast are more prone to have ACS compared to those who live in other regions. Do not have motor vehicles been positively associated with ACS [girls: 2.04 (1.72;2.42); boys 1.85(1.63;2.10)]. Those whom self-reported white was less prone to have ACS compared to their peers from other ethnicities. Those enrolled in private schools [girls: 0.43(0.34;0.54); boys (0.45(0.39;0.53)] and schools setting in rural area [girls: 0.38(0.25;0.57); boys: 0.51(0.37;71)] are less prone to show ACS. In addition, adolescents who accumulated less active time during physical education classes [girls: 0.80(0.66;0.97)] and extracurricular shifts [boys: 0.69(0.60;0.80)] were less prone to have an ACS, compared to their most active peers. Lastly, girls who spent ≤ 2 hours presented fewer odds to have an ACS [0.75(0.63;0.90)]. Conclusions: ACS was most prevalent among those who live in the Southeast region and seems to be negativity associated with the socioeconomic level. Moreover, less active adolescents during both school and leisure time may be more prone to have passive travel go/from school.

Resumo O presente estudo teve como objetivo verificar a contribuição dos diferentes domínios da atividade física na “atividade física total” em adolescentes brasileiros.Trata-se de estudo transversal utilizando dados secundários oriundos da terceira edição da Pesquisa Nacional de Saúde do Escolar – PeNSE, 2015. A amostra foi constituída por 100.497 adolescentes de ambos os sexos cursando o 9° ano do Ensino Fundamental. Utilizou-se um modelo de regressão linear a fim de averiguar o quanto cada domínio contribuiu para a atividade física total, considerando o sexo, tipo de município e região. O domínio que apresentou maior contribuição para “atividade física total” independentemente das variáveis sociodemográficas e ambientais foram as “atividades físicas extraescolares” (R2= 0,60), seguido do “deslocamento ativo” (R2= 0,34), e por fim as “aulas de Educação Física” (R2= 0,23). A contribuição dos diferentes domínios apresentou variações por sexo, tipo de município e região. Conclui-se que as “atividades extraescolares” apresentam a maior contribuição para a “atividade física total”, seguido do “deslocamento ativo” e das “aulas de Educação Física”. Além disso, verifica-se uma variação na contribuição dos domínios por sexo, tipo de município e região.

Abstract The present study aimed to verify the contribution of different physical activity domains to “total physical activity” in Brazilian adolescents. This is a cross-sectional study using secondary data from the third edition of the National School Health Survey - PeNSE, 2015. The sample consisted of 100,497 adolescents of both sexes enrolled in the 9th grade of elementary schools. A linear regression model was used to verify how much each domain contributed to total physical activity, considering gender, type of municipality and region. The domain with the largest contribution to “total physical activity” regardless of sociodemographic and environmental variables was “extra-school physical activity” (R2 = 0.60), followed by “active commuting” (R2 = 0.34), and finally “Physical Education classes” (R2 = 0.23). The contribution of the different domains varied by gender, type of municipality and region, and it was concluded that “extra-school physical activity” make the greatest contribution to “total physical activity”, followed by “active commuting” and “Physical Education classes”. In addition, variation was observed in the contribution of domains by gender, type of municipality and region.

Resumo Objetivou-se verificar a associação entre ambiente escolar e atividade física acumulada em adolescentes brasileiros. Estudo com dados secundários da “Amostra 1” da terceira edição da Pesquisa Nacional de Saúde do Escolar, com 102072 crianças e adolescentes matriculados no nono ano do Ensino Fundamental. Foram abordadas questões sobre características do ambiente escolar e a oferta de oportunidades de práticas de atividade física, com foco nas informações sobre a estrutura da atividade física e sobre a prática regular de atividades físicas acumuladas. As variáveis foram organizadas a partir da estrutura das escolas e oportunidades para a prática de atividades físicas, enquanto a atividade física acumulada foi utilizada como variável desfecho. Os dados foram analisados a partir de um modelo de regressão logística binária, organizado em modelos brutos e ajustados, com nível de significância de 5%. Os dados foram analisados no SPSS for Windows, versão 22.0. Os resultados indicam que, a partir do ajuste das variáveis, houve associação entre a prática de atividade física acumulada e a existência de quadra poliesportiva sob condições de uso (OR=1,22; IC95%=1,18-1,26), ou presença de pátio para uso de aulas de educação física (OR=1,04; IC95%=1,01-1,06). Foi verificada associação entre a oferta de atividades físicas variadas, exceto as aulas de educação física, e a prática de atividade física acumulada (OR=1,05; IC95%=1,02-1,08). A existência de quadra de esportes/pátio nas escolas e a oferta de atividades físicas extracurriculares estão associadas à prática de atividade física acumulada em jovens estudantes brasileiros.

Abstract The aim of this study was to verify the association between school environment and accumulated physical activity in Brazilian adolescents. This is a study with secondary data from sample 1, from the third edition of the National School Health Survey, a study carried out with 102.072 children and adolescents enrolled in the ninth school grade. Questions about characteristics of the school environment and the offer of opportunities for physical activity practices were addressed, focusing on information regarding the structure for physical activity and about the regular practice of accumulated physical activities. Variables were organized from the structure of schools and opportunities for the practice of physical activities, while accumulated physical activity was used as the outcome variable. Data were analyzed from a binary logistic regression model, organized in crude and adjusted models, with a significance level of 5%. Data were analyzed from SPSS for Windows, version 22.0. Results indicate that, from the adjustment of variables, there was an association between the practice of accumulated physical activity and the existence of a sports court under conditions of use (OR=1.22, 95%CI=1.18-1.26), or presence of courtyard for use of physical education classes (OR=1.04, 95%CI=1.01-1.06). Association between the offer of varied physical activities, except for physical education classes, and the practice of accumulated physical activity (OR=1.05, 95%CI=1.02-1.08) was identified. Existence of a sports court/courtyard in schools and the offer of extracurricular physical activities are associated with the practice of accumulated physical activity in young Brazilian students.

Atividades agrícolas, florestais e do setor imobiliário vêm, ao longo do tempo, modificando ambientes ecologicamente estruturados. As consequências são drásticas e, por isso, faz-se necessária a busca por ferramentas e instrumentos para diagnosticar e monitorar a diversidade biológica desses locais. Os insetos têm-se destacado como potenciais organismos bioindicadores e isso se deve ao fato de apresentarem grande capacidade perceptiva, no que se refere a alterações do meio ambiente, principalmente por seu apurado sistema sensorial, que lhes permite qualificar condições ambientais em determinadas situações e, ainda, quantificar danos causados ao meio. Um dos problemas que podem ser associados a este setor é a falta de padronização e definição de protocolos de coleta e avaliação da biodiversidade, para que esses resultados possam ser analisados e extrapolados para diferentes ambientes antropizados. Neste trabalho, é contextualizada a importância da entomofauna como bioindicadora, em ecossistemas.

Agricultural, forestry and real estate activities have been modifying ecologically structured environments. The consequences of these activities are drastic and so it is necessary to search for tools to diagnose and monitor biological diversity of these environments. Insects have been identified as potential biomarkers because of their great ability to perceive changes in the environment, mainly due to their acute sensory system that allows them to evaluate the environmental conditions in certain situations, as well as to quantify damages to the environment. However, there is a lack of standardization and definition of protocols for the collection and assessment of biodiversity so that these results can be analyzed and extrapolated to different anthropogenic environments. This study contextualized the importance of the insect fauna as bioindicators in different ecosystems

Asphaltenes are fractions of crude oils that can precipitate and one of the parameters used in the prediction of the conditions in which this phenomenon occurs is the Hildebrand solubility parameter. In this work, it was evaluated the uncertainty propagation in the experimental determination of the solubility parameter of different crude oils, calculated from data of the asphaltenes precipitation by the addition of n-heptane, identified by optical microscopy. It was verified that the solubility parameter of an oil and the associated uncertainty are specific, being recommended that, whenever viable, it is determined parallel both, conferring higher credibility to the results.

O experimento foi conduzido na Faculdade de Ciências Agronômicas - UNESP/Câmpus de Botucatu (SP), com o objetivo de avaliar o florescimento fora de época e o crescimento vegetativo da tangerineira 'Poncã'. Nesse contexto, adotou-se o delineamento estatístico em blocos casualizados, em parcelas subdivididas, com duas repetições, na instalação do ensaio. Os dois tratamentos de -0,03 e -0,05 MPa, como potenciais mínimos da água no solo, constituíram as parcelas e as quatro doses de paclobutrazol: 0; 4; 8 e 12 g por planta, nas subparcelas. No segundo ano de pesquisa, foram mantidas as mesmas parcelas e foram aplicadas nas plantas das subparcelas as doses de 0; 500; 1000 e 2000 mg l-1 de paclobutrazol, via foliar. Cada parcela foi constituída de 16 plantas, sendo oito destinadas à avaliação. Utilizou-se, em ambas as combinações, de um tratamento sem irrigação (-0,07 MPa) e sem aplicação de paclobutrazol, como testemunha. Foram avaliados parâmetros, como a altura, diâmetro médio, volume e área de projeção da copa e condutância estomática para caracterizar a resposta das plantas aos tratamentos empregados. Concluiu-se que a aplicação do paclobutrazol e a variação do potencial da água no solo não proporcionaram a indução do florescimento fora de época das tangerineiras, e que os níveis de paclobutrazol influenciaram no desenvolvimento das plantas.

The aim of this research was to study the flowering out of season in no induction conditions and the vegetative development of 'Ponkan' mandarin by irrigation and paclobutrazol application. The experiment was carried out at the Faculty of Agricultural Sciences - UNESP/Botucatu, State of São Paulo. The treatments followed a randomized blocks experimental design, being distributed in split-splots, and two replications. Each experimental unit was represented per 16 plants, being 8 destined for the evaluations. The treatments consisted of two soil water potential, -0,03 e -0,05 MPa that constituted the main experimental unit and the paclobutrazol levels that represented the sub units. In the second year of the experiment the units were maintained and it was applied the paclobutrazol: 0; 500; 1000 e 2000 mg L-1, by foliar application, in the plants of the sub units. Both treatments was compared with the treatments without irrigation (control plants: -0,07MPa) and whitout paclobutrazol application plants. It was evaluated the following parameters: height, scion diameter medium, scion projection area, scion volume and stomatic condutance showing the plant's response to treatments. It was concluded that the paclobutrazol application and the irrigation did not influence the plant's flowering out of season and that the paclobutrazol levels influenced the growth of 'Ponkan' mandarin plants.