Pesquisa | SciELO

Abstract Objective Testing an experimental model for ischemic necrosis of the femoral head in Legg-Calvé-Perthes disease by evaluating gait, imaging and morphohistology. Methods The operation was done in 11 piglets. Necrosis by cerclage in the right femoral neck was induced. Piglets were divided into group A, with 8 animals, euthanizing two in the 2nd, 4th, 6th, and 8th weeks, respectively; and group B, with 2 animals (sham), submitted to the surgical procedure without cerclage of the right femoral neck. The gait classification used was that of Etterlin. The frozen femurs were submitted to digital radiography and computed tomography. The height and width of the epiphysis and epiphysary coefficient were measured at study times. Light microscopy and immunohistochemistry with TGF-β1 were performed. Results One animal died of sepsis in Group A. In this group, claudication was observed in all animals. On digital radiography and computed tomography, bone sclerosis, enlargement of the right femoral neck, flattening, collapse, and fragmentation of the right femoral head were observed. All epiphysis height and epiphysary coefficient values of the right femoral head were lower than the contralateral ones, in which were observed chondrocytes disordered and separated by gaps. A reduction in TGF-β1 expression was observed at 2 and 6 weeks in the right femoral head and at eight in the left. In group B, there were no signs of necrosis and gait was normal. Conclusions The model presented reproduced macroscopic necrosis on digital radiography, computed tomography, and microscopy. Gait evaluation showed a good correlation with other ischemia findings. Level of EvidenceV. Diagnostic studies.

Resumo Objetivo Testar um modelo experimental para necrose isquêmica da cabeça femoral na doença de Legg-Calvé-Perthes avaliando a marcha, exames de imagens e morfohistologia. Métodos Operaram-se 11 leitões. Induziu-se a necrose por cerclagem no colo femoral direito. Dividiram-se os leitões em grupo A com 8 animais, sacrificando-se dois na 2ª, 4ª, 6ª e 8ª semanas, respectivamente; e grupo B, com 2 animais (sham), submetidos ao procedimento cirúrgico sem a cerclagem do colo femoral direito. A classificação da marcha utilizada foi a de Etterlin. Os fêmures congelados foram submetidos à radiografia digital e tomografia computadorizada. Mediram-se a altura e largura da epífise e o coeficiente epifisário nos tempos de estudo. Realizou-se, microscopia de luz e imunohistoquímica com TGF-β1. Resultados Um animal morreu por sepse no grupo A. Neste grupo, observou-se claudicação em todos os animais. Na radiografia digital e tomografia computadorizada observaram-se: esclerose óssea, alargamento do colo femoral direito, achatamento, colapso e fragmentação da cabeça femoral direita. Todos os valores da altura da epífise e coeficiente epifisário da cabeça femoral direita foram menores que os contralaterais, nos quais observaram-se condrócitos desordenados e separados por lacunas. Observou-se redução da expressão do TGF-β1 com 2 e 6 semanas nas cabeças femorais direitas e nas esquerdas com oito. No grupo B, não ocorreram sinais de necrose e a marcha foi normal. Conclusões O modelo apresentado reproduziu a necrose macroscopicamente, na radiografia digital, tomografia computadorizada e microscopia. A avaliação da marcha demonstrou boa correlação com os demais achados de isquemia. Nível de EvidênciaV. Estudos diagnósticos.

https://doi.org/10.1055/s-0042-1749419

Diversity of rickettsiae in ticks (Acari: Ixodidae) collected from wild vertebrates in part of the Amazon, Cerrado, and Pantanal biomes in Brazil
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Prati, Anny Carolina

; Maia, Maerle Oliveira

; Martins, Thiago Fernandes

; Morgado, Thaís Oliveira

; Corrêa, Sandra Helena Ramiro

; Mendes, Edson Junior Figueiredo

; Ferraz, Rosa Helena dos Santos

; Mudrek, Jessica Rhaiza

; Strüssmann, Christine

; Ramos, Dirceu Guilherme de Souza

; Semedo, Thiago Borges Fernandes

; Minetto, Make Kawatake

; Aguiar, Daniel Moura de

; Pacheco, Richard Campos

; Melo, Andréia Lima Tomé

Revista Brasileira de Parasitologia Veterinária

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2023, Volume 32 Nº 4 elocation e008023

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Ticks parasitizing 102 wild animals in the states of Mato Grosso and Goiás, Brazil were collected between 2015 and 2018. A total of 2338 ticks (865 males, 541 females, 823 nymphs, and 109 larvae) belonging to four genera (Amblyomma, Dermacentor, Haemaphysalis, and Rhipicephalus) and at least 21 species were identified. DNA extraction and a molecular survey for rickettsial agents were performed on 650 ticks. The results revealed parasitism by the following species: Rickettsia amblyommatis in Amblyomma cajennense s.s., A. cajennense s.l., Amblyomma coelebs, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, Amblyomma scalpturatum, Amblyomma sculptum, and Amblyomma romitii; Rickettsia parkeri in Amblyomma nodosum, Amblyomma ovale, Amblyomma scalpturatum, and Amblyomma triste; Rickettsia rhipicephali in Haemaphysalis juxtakochi; Rickettsia sp. in A. cajennense s.s., A. nodosum, and A. sculptum, and lastly, ‘Candidatus Rickettsia andeanae’ in Amblyomma parvum and Rhipicephalus microplus. This study expands the body of knowledge about tick parasitism among wild animals, including new data concerning tick-host associations, and provides information about the epidemiology of tick-borne pathogens in the Center-West region of Brazil.

Resumo Foram coletados carrapatos parasitando 102 animais silvestres nos estados de Mato Grosso e Goiás, Brasil, entre 2015 e 2018. Um total de 2338 carrapatos (865 machos, 541 fêmeas, 823 ninfas e 109 larvas) pertencentes a quatro gêneros (Amblyomma, Dermacentor, Haemaphysalis e Rhipicephalus) e pelo menos 21 espécies foram identificadas. A extração de DNA e pesquisa molecular para agentes riquétsiais foram realizadas em 650 carrapatos. Os resultados revelaram parasitismo pelas seguintes espécies: Rickettsia amblyommatis em Amblyomma cajennense s.s., A. cajennense s.l., Amblyomma coelebs, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, Amblyomma scalpturatum, Amblyomma sculptum e Amblyomma romitii; Rickettsia parkeri em Amblyomma nodosum, Amblyomma ovale, Amblyomma scalpturatum e Amblyomma triste; Rickettsia rhipicephali em Haemaphysalis juxtakochi; Rickettsia sp. em A. cajennense s.s., A. nodosum e A. sculptum e, por último, ‘Candidatus Rickettsia andeanae’ em Amblyomma parvum e Rhipicephalus microplus. Este estudo amplia o conhecimento sobre o parasitismo de carrapatos em animais silvestres, incluindo-se novos dados sobre associações carrapato-hospedeiro. Fornece, ainda, informações sobre a epidemiologia de patógenos transmitidos por carrapatos na região Centro-Oeste do Brasil.

https://doi.org/10.1590/s1984-29612023059

Deep sea ecosystem exploration and ‘health check’: sampling strategy and methods applied during the iAtlantic_BR10_Petrobras cruise in the Santos Basin, Southwest Atlantic
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Perez, Jose Angel A.

; Barros Neto, Halésio M.C. de

; Arantes, Renata C.M.

; Gaurisas, Daniela Y.

; Silva, Camila F.

; Alves, Fernanda Maria M.

; Costa, Júlia A.

; Eloi, Paula D.C.

; Fonseca, Thayse S.

; Gavazzoni, Lucas

; Lonskis, Isabella da S.

; Nardi, Ricardo U.

; Nascimento, Pedro O.

; Rodrigues, João Vitor M.

; Santos, Ana Lara F. dos

; Santos, Edson C. dos

; Schroeder, Rafael

; Silva, Luis Carlos da

; Souza, Fernanda dos S.S. de

; Bernardino, Ângelo F.

; Cavalcanti, Guarani H.

; Lindner, Alberto

; Mahiques, Michel M. de

; Millo, Christian

; Reis, Priscila

; Sweetman, Andrew K.

; Roberts, J. Murray

Ocean and Coastal Research

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2023, Volume 71 elocation e23046

Resumo: En | Texto: En | PDF: En

Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

Sowing date changes phenological development, plastochron index, and grain yield of soybeans under Cerrado conditions
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Bossolani, João W.

; Meneghette, Hugo H. A.

; Sanches, Izabela R.

; Santos, Fabiana L. dos

; Parra, Lucas F.

; Lazarini, Edson

Revista Brasileira de Engenharia Agrícola e Ambiental

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Jul 2022, Volume 26 Nº 7 Páginas 488 - 494

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A época de semeadura é a prática de manejo cultural com maior interferência no desenvolvimento e na produtividade de grãos de soja, e está diretamente relacionada com o genótipo e tipo de ciclo da cultura. Nosso objetivo foi avaliar o desenvolvimento fenológico em função das respostas fotoperíodicas, índice de plastocrono e produtividade de grãos de três cultivares de soja com ciclos diferentes, semeadas em três épocas distintas O estudo foi realizado em Sevíria, Mato Grosso do Sul e utilizou um delineamento de parcelas subdivididas com as parcelas principais dispostas em blocos com quatro repetições. As parcelas foram compostas por três épocas de semeadura com intervalo de 15 dias, a partir de 15 de novembro, e as subparcelas foram compostas por três cultivares: BMX Turbo RR (ciclo extra-precoce), BMX Potência RR (ciclo precoce) e TMG 1180 RR (ciclo médio). A semeadura tardia aumentou o índice de plastocrono e reduziu a duração do ciclo de crescimento, altura da planta, número de nós no caule principal e número de vagens por planta. Nossos resultados demonstram que cultivares com ciclos mais longos são mais adequadas para semeadura tardia, apresentam melhor desenvolvimento vegetativo e reprodutivo e maior rendimento de grãos. A segunda época de semeadura foi a mais adequada para o cultivo da soja na região.

ABSTRACT The sowing date is a crop management practice that affects soybean development and grain yield, and is directly related to the genotype and cycle type. Our objective was to evaluate phenological development as a function of photoperiodic responses, plastrochron index, and grain yield in three soybean cultivars with different growth cycles sown on three sowing dates. The study was conducted in Selvíria, Mato Grosso do Sul State, Brazil, using a split-plot design with the main plots arranged in blocks with four replications. The main plots included three sowing dates, 15 days apart, beginning on November 15, and the subplots were composed of three cultivars: BMX Turbo RR (extra-early cycle), BMX Potência RR (early cycle), and TMG 1180 RR (medium cycle). Delayed sowing increased the plastochron index and reduced the growth cycle duration, plant height, node number of the main stem, and pod number per plant. We found that cultivars with longer cycles were more suitable for delayed sowing, had improved vegetative and reproductive development, and had higher grain yields than those with shorter periods. The second sowing date was most suitable for soybean cultivation in this region.

https://doi.org/10.1590/1807-1929/agriambi.v26n7p488-494

LOW-COST ELECTRO-MECHANICAL PRESS FOR DETERMINING PRE-CONSOLIDATION PRESSURE
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Duarte, Thiago F.

; Godoy, Edson

; Silva, Tonny J. A. da

; Bonfim-Silva, Edna M.

; Santos, Douglas V. R. dos

Engenharia Agrícola

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2022, Volume 42 Nº 3 elocation e20210227

Resumo: En | Texto: En | PDF: En

ABSTRACT The pre-consolidation pressure is a soil parameter indicating the maximum load that can be supported without irreversible deformation. However, the commercially available equipment for such determination is very expensive, limiting studies on this subject. The objective of this study was to develop a low-cost press model for determining a soil pre-consolidation pressure. The press comprised an electric jack for lifting a soil sample against a fixed piston. A compression cell was built using Technyl®. The stability and smoothness of the applied loads were controlled using springs with different dimensions and strengths. The load and deformation data were obtained using an indicator connected to the load cell and a dial indicator, respectively. The equipment was tested by determining the pre-consolidation pressure in an Oxisol cultivated with cotton and under an area of native Cerrado. The pre-consolidation pressures for a soil water tension of 10 kPa were 50.8 kPa and 61.1 kPa for the soil under the native Cerrado and cotton crop, respectively, and the compressibility indexes were 0.71 and 0.37, respectively. The main advantages of this equipment are its low cost, ease of construction, and simplicity of operation. However, the equipment is non-automatic; thus, the data necessary for the construction of the compression curve must be collected by an operator.

https://doi.org/10.1590/1809-4430-eng.agric.v42n3e20210227/2022

Occurrence of Rust in Myrcianthes pungens (O. BERG) D. Legrand Caused by Austropuccinia psidii in The State of Rio Grande do Sul
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Santos, Rodrigo Areze da Silva

; Schwarz, Sergio Francisco

; Silva, Magnólia Aparecida Silva da

; Bertolini, Edson

; Andrade, Camila Cristina Lage de

; Pacini, Bruna Alana Haupt

Revista Brasileira de Fruticultura

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2022, Volume 44 Nº 5 elocation e-073

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Guabiju (Myrcianthes pungens), pertencente à família Myrtaceae, com ampla ocorrência no Rio Grande do Sul (RS), apresenta grande potencial para exploração de seus frutos no consumo in natura, com sabor adocicado, podendo compor bebidas, geleias e sorvetes, além de possuir propriedades nutracêuticas. As ferrugens, como característica principal, apresentam a formação de pústulas alaranjadas contendo urediniósporos do patógeno sobre os órgãos afetados. A ação do patógeno causa deformação dos órgãos: caules, folhas, flores e frutos, interferindo, assim, nos processos fisiológicos da planta. Assim, o presente trabalho teve como objetivo apresentar informações sobre a ocorrência, confirmação do agente causal da ferrugem do guabijuzeiro, além de relatar os acessos mais suscetíveis e resistentes a Austropuccinia na coleção de trabalho de guabijuzeiros em Eldorado do Sul-RS. A diagnose da doença foi realizada com base nos sintomas, na observação das estruturas fúngicas por microscopia óptica e por análises moleculares. A partir da microscopia, observaram-se urediniósporos elipsoides a ovoides e levemente equinulados, característicos de Austropuccinia sp. A sequência da região internal transcribed spacer(ITS) do isolado apresentou 99,06% de similaridade com sequências da mesma região da espécie A. psidii depositadas no banco de dados nucleotídeos - GenBank (NCBI). Este é o primeiro relato de ferrugem associada a guabijuzeiros no Estado do Rio Grande do Sul, Brasil. A partir da escala diagramática desenvolvida, foi possível identificar diferentes níveis de suscetibilidade a A. psidii nos acessos de guabijuzeiros avaliados.

Abstract Guabiju tree (Myrcianthes pungens) belongs to the Myrtaceae family, with wide occurrence in Rio Grande do Sul (RS), southern Brazil, demonstrates great commercial potential regarding the consumption of its fresh fruit, which has a sweet taste and can be used in drinks, jellies and ice creams, in addition to its nutraceutical properties. As their main characteristic, rusts present the formation of orange pustules containing urediniospores of the pathogen on affected organs. The action of the pathogen causes deformation of stems, leaves, flowers and fruits, thus interfer-ing with the physiological processes of the plant. Thus, the present work aimed at presenting information on the occurrence and confirmation of the causal agent of guabiju rust, in addition to reporting the accessions most susceptible and resistant to Austropuccinia in the guabiju working collection of Eldorado do Sul, RS, Brazil. The diagnosis of the disease was based on symptoms, observation of fungal structures by light microscopy and molecular analyses. From microscopy, ellipsoid to ovoid and slightly equinulate urediniospores were observed, characteristic of Austropuccinia sp. The sequence of the internal transcribed spacer (ITS) region of the isolate showed 99.06% similarity with sequences from the same region of A. psidii deposited on the nucleotide database - GenBank (NCBI). This is the first report of rust associated with guabiju in the state of Rio Grande do Sul, Brazil. From the diagrammatic scale developed, it was possible to identify different levels of susceptibility to A. psidii in guabiju accessions under study.

https://doi.org/10.1590/0100-29452022073

Botanical sources and heavy metals contents of honey produced by Apis mellifera in an ecotone region of the state of Bahia, Brazil
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SILVEIRA-JÚNIOR, CRISTIANO EDUARDO A.

; LOPES, BIANCA A.

; SILVA, TÂNIA MARIA S.

; GOMES, AYALA NARA P.

; SILVA, GIRLIANE REGINA DA

; ARRUDA, RAFAEL S. DE

; MACIEL, EDSON A.

; SANTOS, FRANCISCO A.R. DOS

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Supl. 4 elocation e20211551

Resumo: En | Texto: En | PDF: En

Abstract The present study investigated by palynological and chemical analysis (Flame Atomic Absortion Spectrometry) about the botanical origin and the heavy metals content (arsenic, cadmium, chromium, lead and mercury) of monthly honey samples of Apis mellifera L. over two years. The pollen types Apiaceae, Mimosa caesalpiniifolia, M. tenuiflora and Myrcia indicated the main floristic sources used by bees. M. tenuiflora was the most frequent of the pollen types, and because it predominates in different months in each year, which may indicate more than one species of the genus being foraged by the beehive. The climatic influence (rainfall and temperature) on the pollen diversity was investigated and was not statistically supported. The chemical analysis showed that the heavy metal content of the samples were below their respective limits of quantification, and, therefore, the samples can be considered safe for human consumption.

https://doi.org/10.1590/0001-3765202220211551

Agronomic traits and chemical composition of forage sorghum plants fertilized with poultry litter and fermentative profile of silages
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Lucena, Nagnaldo T.

; Santos, Edson M.

; Oliveira, Juliana S.

; Perazzo, Alexandre F.

; Cruz, Gabriel F.L.

; Pereira, Danillo M.

; Pereira, Gildênia A.

; Macêdo, Alberto J.S.

; Ramos, Rosângela C.S.

; Nogueira, Messias S.

Chilean journal of agricultural research

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Dez 2021, Volume 81 Nº 4 Páginas 575 - 584

Resumo: En | Texto: En | PDF: En

ABSTRACT It is essential to determine the productive potential and quality of forage sorghum (Sorghum bicolor (L.) Moench) silages using alternative and organic inputs, such as poultry litter (PL), intending to higher efficiency, and sustainable development of animal production systems in the world. Poultry litter fertilization has been widely used in crops for silage production, where it is believed that fertilization with PL can influence the fermentation profile and composition of forage sorghum silages. The objective of this study was to evaluate different levels of fertilization with PL on the agronomic and quality characteristics of forage sorghum silage ‘BRS Ponta Negra’. For determination of agronomic and productive characteristics, randomized blocks design was adopted, the treatments were different PL levels: 5, 10, and 15 Mg ha-1. While for the evaluation of silage characteristics, the design was completely randomized. With fertilizer doses, a linear increase was observed for plant height, stem diameter, leaf length, and consequently for fresh and DM productions. Linear reduction was observed for neutral detergent fiber, while crude protein and pH contents of silages increased linearly with PL levels. Quadratic effect occurred for gas and effluent losses. Poultry litter may be an alternative source to supply the N needs in the production of sorghum for silage under experimental similar conditions. Associating productivity with cultivation efficiency, the PL level 13.46 Mg ha-1 is recommended for presenting the highest N agronomic efficiency and good nutritional quality of the silage.

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Diretrizes da Sociedade Brasileira de Cardiologia sobre Angina Instável e Infarto Agudo do Miocárdio sem Supradesnível do Segmento ST – 2021
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Nicolau, José Carlos

; Feitosa Filho, Gilson Soares

; Petriz, João Luiz

; Furtado, Remo Holanda de Mendonça

; Précoma, Dalton Bertolim

; Lemke, Walmor

; Lopes, Renato Delascio

; Timerman, Ari

; Marin Neto, José A.

; Bezerra Neto, Luiz

; Gomes, Bruno Ferraz de Oliveira

; Santos, Eduardo Cavalcanti Lapa

; Piegas, Leopoldo Soares

; Soeiro, Alexandre de Matos

; Negri, Alexandre Jorge de Andrade

; Franci, Andre

; Markman Filho, Brivaldo

; Baccaro, Bruno Mendonça

; Montenegro, Carlos Eduardo Lucena

; Rochitte, Carlos Eduardo

; Barbosa, Carlos José Dornas Gonçalves

; Virgens, Cláudio Marcelo Bittencourt das

; Stefanini, Edson

; Manenti, Euler Roberto Fernandes

; Lima, Felipe Gallego

; Monteiro Júnior, Francisco das Chagas

; Correa Filho, Harry

; Pena, Henrique Patrus Mundim

; Pinto, Ibraim Masciarelli Francisco

; Falcão, João Luiz de Alencar Araripe

; Sena, Joberto Pinheiro

; Peixoto, José Maria

; Souza, Juliana Ascenção de

; Silva, Leonardo Sara da

; Maia, Lilia Nigro

; Ohe, Louis Nakayama

; Baracioli, Luciano Moreira

; Dallan, Luís Alberto de Oliveira

; Dallan, Luis Augusto Palma

; Mattos, Luiz Alberto Piva e

; Bodanese, Luiz Carlos

; Ritt, Luiz Eduardo Fonteles

; Canesin, Manoel Fernandes

; Rivas, Marcelo Bueno da Silva

; Franken, Marcelo

; Magalhães, Marcos José Gomes

; Oliveira Júnior, Múcio Tavares de

; Filgueiras Filho, Nivaldo Menezes

; Dutra, Oscar Pereira

; Coelho, Otávio Rizzi

; Leães, Paulo Ernesto

; Rossi, Paulo Roberto Ferreira

; Soares, Paulo Rogério

; Lemos Neto, Pedro Alves

; Farsky, Pedro Silvio

; Cavalcanti, Rafael Rebêlo C.

; Alves, Renato Jorge

; Kalil, Renato Abdala Karam

; Esporcatte, Roberto

; Marino, Roberto Luiz

; Giraldez, Roberto Rocha Corrêa Veiga

; Meneghelo, Romeu Sérgio

; Lima, Ronaldo de Souza Leão

; Ramos, Rui Fernando

; Falcão, Sandra Nivea dos Reis Saraiva

; Dalçóquio, Talia Falcão

; Lemke, Viviana de Mello Guzzo

; Chalela, William Azem

; Mathias Júnior, Wilson

Arquivos Brasileiros de Cardiologia

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Jul 2021, Volume 117 Nº 1 Páginas 181 - 264

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20210180 3052 downloads

10.

Cross-cultural adaptation of the Internet Gaming Disorder Scale – Short Form (IGDS9-SF) to the Brazilian context
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Donadon, Mariana F.

; Chagas, Marcos H. N.

; Apolinário-da-Silva, Thiago D.

; Okino, Erika T. K.

; Hallak, Jaime E. C.

; Nicoletti, Êdela A.

; Pereira-Lima, Karina

; Degan, Edson A.

; Santos, Rafael G.

; Machado-de-Sousa, João Paulo

; Simei, João L. Q.

; Oliveira, Lucas M.

; Pontes, Halley M.

; Osório, Flávia L.

Trends in Psychiatry and Psychotherapy

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Set 2020, Volume 42 Nº 3 Páginas 262 - 266

Resumo: En | Texto: En | PDF: En

Abstract Introduction The Internet Gaming Disorder Scale – Short Form (IGDS9-SF) assesses the severity, harmful effects and/or consequences of excessive online and offline gaming. Its conciseness and theoretical foundations on current diagnostic criteria of gaming disorders make it a useful resource for clinical and screening settings. Objective To describe the process of cross-cultural adaptation of the IGDS9-SF to the Brazilian context. Methods The cross-cultural adaptation involved the steps of independent translation of the instrument, synthesis version, back-translation, pre-test and elaboration of the final version. Content validity assessment was conducted by a multidisciplinary committee of experts and consisted of both a quantitative analysis (calculation of content validity coefficients – CVC) and a qualitative analysis (assessment of the experts’ comments and suggestions). The pre-test sample consisted of 30 gamers with variable sociodemographic characteristics. Results The cross-cultural adaptation of the scale followed the proposed protocol, and the CVC was satisfactory (≥ 0.83) for all the structures and equivalences assessed. Most of the suggestions made by the experts were accepted (mainly adjustments and language standardization). The gamers who participated in the pre-test judged the scale easy to understand and did not suggest changes. Discussion The Brazilian version of the IGDS9-SF showed adequate content validity and is available for researchers and clinicians, as well as for the investigation of additional psychometric characteristics.

https://doi.org/10.1590/2237-6089-2019-0032 505 downloads

11.

Granulomatous pneumonia due to pythiosis in captive South American coatis (Nasua nasua)
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Bezerra, Kalinne S.

; Santos, Tarcísio A.

; Rosa, Janaina M.A.

; Pescador, Carolina A.

; Dutra, Valeria

; Nakazato, Luciano

; Driemeier, David

; Colodel, Edson M.

Pesquisa Veterinária Brasileira

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Ago 2020, Volume 40 Nº 8 Páginas 647 - 650

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: A pitiose é uma doença infecciosa emergente que afeta animais silvestres de cativeiro e em vida livre. Reportamos dois casos de pneumonia granulomatosa decorrentes da infecção por Pythium Insidiosum em quatis sul-americanos (Nasua nasua), que foram encontrados mortos sem apresentar nenhum quadro clínico prévio. Pneumonia granulomatosa severa associada a efusão pleural foi observada durante a necropsia. Na microscopia, foram observados múltiplos granulomas e piogranulomas de tamanhos variados que continham imagens negativas de hifas na periferia de seus centros necróticos. A coloração de metenamina de prata (Grocott) evidenciou estas estruturas, e a imunomarcação (anti-P. insidiosum) foi fortemente positiva. A análise molecular pela reação de polimerase em cadeia amplificou o DNA específico do P. insidiousum. Estes achados caracterizaram o P. insidiosum como a causa da pneumonia granulomatosa nos quatis e provou que a pitiose deve ser considerada um diagnostico diferencial para outras doenças respiratórias que afetam esta espécie.

ABSTRACT: Pythiosis is an emerging infectious disease affecting captive and free-ranging wild animals. We report granulomatous pneumonia due to Pythium insidiosum in two South American coatis (Nasua nasua), who were found dead without any clinical records. Severe granulomatous pneumonia associated with pleural effusion was revealed in the necropsy. Microscopically, variably sized granulomas and pyogranulomas presented negative hyphae profiles at the periphery of their necrotic cores. Grocott methenamine silver stain highlighted these structures, and immunostain (anti- P. insidiosum) was strongly positive. Molecular analysis by polymerase chain reaction amplified P. insidiosum specific DNA. These findings characterized P. insidiosum as a cause of granulomatous pneumonia in coatis and proved that pythiosis needs to be considered in the differential diagnosis of respiratory diseases affecting this species in endemic areas.

https://doi.org/10.1590/1678-5150-pvb-6459 550 downloads

12.

AVALIAÇÃO DA BIOMASSA DE FUNGOS AMAZÔNICOS COMO FONTE DE LIPASES PARA BIOCATÁLISE
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Romano, Israel P.

; Santos, Vanderlei S. dos

; Louzada, Ana Carolina de Lima Paes

; Pereira Junior, Raimundo C.

; Carmo, Edson J. do

; Mota, Adolfo José da

; Barroso, Hiléia dos S.

; Itabaiana Junior, Ivaldo

; Pereira, José Odair

; Astolfi Filho, Spartaco

; Zanotto, Sandra Patrícia

Química Nova

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Fev 2020, Volume 43 Nº 2 Páginas 146 - 154

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.

https://doi.org/10.21577/0100-4042.20170470 600 downloads

13.

Genome size and identification of repetitive DNA sequences using low coverage sequencing in Hancornia speciosa Gomes (Apocynaceae: Gentianales)
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Santos, Vanessa

; Silva, Edson Ferreira da

; Almeida, Cícero

Genetics and Molecular Biology

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2020, Volume 43 Nº 4 elocation e20190175

Resumo: En | Texto: En | PDF: En

Abstract Repetitive DNA is an important component of eukaryotic genomes, accounting for more than 90% of the genome size of some species, including mobile elements and satellite DNA sequences. The aim of study was to characterize the genome of Hancornia speciosa Gomes using C-value genome size estimate and repetitive DNA sequences analysis. The genome size estimate was obtained by flow cytometry and the repetitive DNA sequences were accessed using graph-based clustering. Evolutionary relationships among species of Apocynaceae was obtained using reads of Catharanthus roseus L., Rhayza stricta Decne, and Asclepias syriaca L. from the NCBI and analyzed by graph-based clustering. The genome size estimates in two botanical varieties showed 2C-values ranging from 0.88 to 1.08 pg, indicating small genome size. Clusters representing repeats making up at least 0.01% of the genome revealed the proportion of repetitive DNA ranging from 19.87% (H. speciosa) to 51.674% (A. syriaca), of which the mobile elements were more abundant. Satellite DNA sequences were not found in H. speciosa and R. stricta, while at least one satellite was detected in C. roseus and A. syriaca, suggesting that the LTR retrotransposon Ty3/Gypsy/Chromovirus may have replaced the satellite DNA in H. speciosa and R. stricta.

https://doi.org/10.1590/1678-4685-gmb-2019-0175 555 downloads

14.

Biology of Bactrocera carambolae (Diptera: Tephritidae) on four hosts
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Castilho, Alison Pureza

; Pasinato, Joel

; Santos, Jhulie Emille Veloso dos

; Costa, Analia e Silva da

; Nava, Dori Edson

; Jesus, Cristiane Ramos de

; Adaime, Ricardo

Revista Brasileira de Entomologia

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Dez 2019, Volume 63 Nº 4 Páginas 302 - 307

Resumo: En | Texto: En | PDF: En

ABSTRACT Bactrocera carambolae is a quarantine pest found in Brazil, restricted to the states of Amapá, Pará and Roraima. This fruit fly can potentially cause extensive socioeconomic and environmental damage in the country, if it disperse into areas where fruit is grown for exporting. The objective of this work was to study the biology of B. carambolae on fruits of Averrhoa carambola L. (Oxalidaceae), Psidium guajava L. (Myrtaceae), Spondias mombin L. (Anacardiaceae) and Eugenia stipitata McVaugh (Myrtaceae). The following parameters were investigated: duration of egg-larva, pupal, egg-adult, pre-oviposition, oviposition and post-oviposition periods, pupal weight and viability, sex ratio, fecundity, fertility and longevity. All parameters except pupal weight, oviposition and post-oviposition period, egg fertility and sex ratio were influenced by the host plant on which the larvae were reared. The carambola fruit fly completes its development on all those hosts studied here, with the highest fecundities on A. carambola and P. guajava.

https://doi.org/10.1016/j.rbe.2019.09.002 427 downloads

15.

Guideline of the Brazilian Society of Cardiology on Telemedicine in Cardiology - 2019
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Lopes, Marcelo Antônio Cartaxo Queiroga

; Oliveira, Gláucia Maria Moraes de

; Ribeiro, Antonio Luiz Pinho

; Pinto, Fausto J.

; Rey, Helena Cramer Veiga

; Zimerman, Leandro Ioschpe

; Rochitte, Carlos Eduardo

; Bacal, Fernando

; Polanczyk, Carisi Anne

; Halperin, Cidio

; Araújo, Edson Correia

; Mesquita, Evandro Tinoco

; Arruda, José Airton

; Rohde, Luis Eduardo Paim

; Grinberg, Max

; Moretti, Miguel

; Caramori, Paulo Ricardo Avancini

; Botelho, Roberto Vieira

; Brandão, Andréa Araújo

; Hajjar, Ludhmila Abrahão

; Santos, Alexandre Fonseca

; Colafranceschi, Alexandre Siciliano

; Etges, Ana Paula Beck da Silva

; Marino, Bárbara Campos Abreu

; Zanotto, Bruna Stella

; Nascimento, Bruno Ramos

; Medeiros, Cesar Rocha

; Santos, Daniel Vitor de Vasconcelos

; Cook, Daniela Matos Arrowsmith

; Antoniolli, Eduardo

; Souza Filho, Erito Marques de

; Fernandes, Fábio

; Gandour, Fabio

; Fernandez, Francisco

; Souza, Germano Emilio Conceição

; Weigert, Guilherme de Souza

; Castro, Iran

; Cade, Jamil Ribeiro

; Figueiredo Neto, José Albuquerque de

; Fernandes, Juliano de Lara

; Hadlich, Marcelo Souza

; Oliveira, Marco Antonio Praça

; Alkmim, Maria Beatriz

; Paixão, Maria Cristina da

; Prudente, Maurício Lopes

; Aguiar Netto, Miguel A. S.

; Marcolino, Milena Soriano

; Oliveira, Monica Amorim de

; Simonelli, Osvaldo

; Lemos Neto, Pedro A.

; Rosa, Priscila Raupp da

; Figueira, Renato Minelli

; Cury, Roberto Caldeira

; Almeida, Rodrigo Coelho

; Lima, Sandra Regina Franco

; Barberato, Silvio Henrique

; Constancio, Thiago Inocêncio

; Rezende, Wladimir Fernandes de

Arquivos Brasileiros de Cardiologia

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Nov 2019, Volume 113 Nº 5 Páginas 1006 - 1056

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190205 5274 downloads

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