Pesquisa | SciELO

Abstract Chemical and pharmacological potentials of botanical species found in the Cerrado are well-known and widely studied. Chemical diversity of secondary metabolites produced by plants that belong to this Brazilian biome has triggered several studies in the fields of farming, pharmaceutical and cosmetic industries. Therefore, this study aimed at evaluating the chemical composition of essential oils (EOs) from fresh leaves of three species found in the Brazilian Cerrado: Cardiopetalum calophyllum Schltdl. (EO-CC), Campomanesia adamantium (Cambess.) O. Berg (EO-CA) and Protium ovatum Engl. (EO-PO) and at determining their anti-Malassezia furfur and anti-inflammatory activities. Both GC-FID and GC-MS showed that the most abundant chemical class of the oils was the one of sesquiterpene hydrocarbons. The major constituents identified in EO-CC were germacrene D (34.9%) and bicyclogermacrene (26.8%). EO-CA exhibited mainly germacrene D (21.7%) while EO-PO had high concentrations of germacrene D (25.0%) and γ-muurolene (18.6%). EOs were tested by the broth microdilution method on 96-well microplates and exhibited satisfactory activity against M. furfur, i. e., EO-CA had MIC = 375 µg/mL while EO-CC and EO-PO had MIC = 750 µg/mL. The chemotaxis model, which was used for evaluating their anti-inflammatory activity, showed that EOs exhibited effective results: C. calophyllum (EO-CC; IC50 = 24.4 µg/mL), C. adamantium (EO-CA; IC50 = 15.7 µg/mL) and P. ovatum (EO-PO; IC50 = 32.5 µg/mL). In short, biological activities of EO-CC, EO-CA and EO-PO, such as anti-Malassezia furfur and anti-inflammatory ones, were investigated and described for the first time.

https://doi.org/10.1590/1678-4324-2023220034

Spatial and temporal distribution and bioinsecticides control of olive leaf moth in an olive orchard in Southwest of Paraná, Brazil
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Carmona, Gilberto Cesar Carmona

; Andrade, Gilberto Santos

; Citadin, Idemir

; Pertille, Rafael Henrique

; Campos, José Ricardo da Rocha

; Rohr, Angela

; Danner, Moeses Andrigo

Ciência Rural

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2023, Volume 53 Nº 8 elocation e20220008

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT: We verified the spatial and temporal distribution of the olive leaf moth (Palpita forficifera Munroe, 1959) in a new potential region for olive cultivation and evaluated the bioinsecticides effectiveness to control this pest. The experimental orchard composed by Koroneiki and Arbequina cultivars was planted in Pato Branco, Southwest region of Paraná, Brazil. In the field, larvae counts were performed weekly on 25 plants of each cultivar, for 12 months, to verify the spatial and temporal distribution. Furthermore, in laboratory conditions, second instar larvae of P. forficifera were fed with olive leaves treated with Azadirachta indica oil, Bacillus thuringiensis, Metarhizium anisopliae and Beauveria bassiana, and the effectiveness in the larvae mortality was used to compare four bioinsecticides and control. The P. forficifera larvae occurred mainly during the three summer months, had a host preference for the ‘Arbequina’ (68.9% of total larvae) and occurred at random distribution patterns throughout the orchard. The A. indica oil and B. thuringiensis both caused almost 100% larvae mortality under laboratory conditions.

RESUMO: Os objetivos deste trabalho foram verificar a distribuição espacial e temporal da lagarta-da-oliveira (Palpita forficifera Munroe, 1959) em uma nova região com potencial de cultivo de oliveira e avaliar a eficiência de controle desta praga com bioinseticidas. O pomar experimental com as cultivares Koroneiki e Arbequina foi implantado em Pato Branco, região Sudoeste do Paraná, Brasil. No pomar foram realizadas contagens semanais de lagartas em 25 plantas de cada cultivar, durante 12 meses, para verificar a distribuição espacial e temporal. Além disso, em condições de laboratório, larvas de segundo instar foram submetidas à alimentação com folhas de oliveira tratadas com óleo de neem (Azadirachta indica), Bacillus thuringiensis, Metarhizium anisopliae e Beauveria bassiana, e a efetividade de mortalidade das larvas foi utilizada para comparação entre os quatro bioinseticidas e a testemunha. As lagartas de P. forficifera ocorreram concentradas durante os três meses de verão, tiveram preferência hospedeira pela cultivar Arbequina (68,9% do total de larvas registradas), e ocorreram em padrão de distribuição aleatório ao longo do pomar. A aplicação de óleo de neem (A. indica) ou de B. thuringiensis causaram quase 100% de mortalidade das larvas em condições de laboratório.

https://doi.org/10.1590/0103-8478cr20220008

Amarante P. Loucura e transformação social: autobiografia da reforma psiquiátrica no Brasil
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Santos, João Henrique de Sousa

; Passos, Izabel Christina Friche

Saúde em Debate

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Jun 2022, Volume 46 Nº 133 Páginas 585 - 587

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.1590/0103-1104202213323

Amarante P. Loucura e transformação social: autobiografia da reforma psiquiátrica no Brasil
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Santos, João Henrique de Sousa

; Passos, Izabel Christina Friche

Saúde em Debate

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Abr 2022, Volume 46 Nº 133 Páginas 585 - 587

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.1590/0103-1104202213323

Cascade Modeling of the Measuring System Used to Assess S-Parameters of Anchor Rods on Power Transmission Lines Guyed Towers
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Tarragô, V. L.

; Melo, M. T. de

; Novo, Lauro R. G. S. Lourenço

; Barbosa, Douglas C. P.

; Coutinho, Marcelo. S.

; de Medeiros, Luiz H. A.

; Alves, Marcelo M.

; dos Santos, Renan G. M.

; Lott Neto, Henrique B. D. T.

; Gama, Paulo H. R. P.

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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Mar 2022, Volume 21 Nº 1 Páginas 35 - 47

Resumo: En | Texto: En | PDF: En

Abstract The structural condition of the cable-stayed towers anchorage on power transmission lines requires constant monitoring. Maintenance routines must be able to identify faulty anchor rods and substitute them to avoid tower collapses and power delivery interruptions. Modern statistical diagnostic systems based on machine learning requires the generation of several distinct rod sample signals to be trained, which is a time-consuming process in typical electromagnetic solvers. Aiming to generate samples in a feasible time span, this paper presents a modeling strategy based on the cascade analysis of the transmission line composed by the anchor and the reference rods, interconnected by a dedicated high-frequency connector. Each distinct transmission line part is modeled by a quadrupole as a partial ABCD matrix. The complete response for each experimental setup is obtained from the cascaded-multiplying of the partial matrices. The proposed modeling proved to be accurate and provides a faster way to obtain the S-parameters from distinct faulty rods if compared to the traditional methodologies using electromagnetic tools.

https://doi.org/10.1590/2179-10742022v21i1253757

Extracellular hydrolytic enzymes produced by yeasts from Antarctic lichens
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DA SILVA, MAYANNE KARLA

; DA SILVA, AVERLANE V.

; FERNANDEZ, PAULA M.

; MONTONE, ROSALINDA C.

; ALVES, RODRIGO P.

; DE QUEIROZ, ALINE C.

; DE OLIVEIRA, VALÉRIA M.

; DOS SANTOS, VIVIANE P.

; PUTZKE, JAIR

; ROSA, LUIZ HENRIQUE

; DUARTE, ALYSSON W.F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Supl. 1 elocation e20210540

Resumo: En | Texto: En | PDF: En

Abstract In the Antarctic environment, yeasts are versatile eukaryotes that have shown wide dispersion in different substrates, producing active enzymes in extreme conditions, but their relevance in biotechnological applications is largely unknown. The aim of this study was to evaluate the production of extracellular hydrolases by yeasts isolated from Antarctic lichens and molecularly identify these isolates. From a total of 144 isolates on the screening, 109 (76%) produced at least one of the hydrolases tested, with most activities for proteases 59 (41%), cellulases 58 (40%), esterases 57 (39%), lipases 29 (20%), amylases 23 (16%) and pectinases 20 (14%). Among these isolates, 76 were identified, most belonged to the phylum Basidiomycota (n=73) with the dominance of Vishniacozyma victoriae (n=27), Cystobasidium alpinum (n=3), Mrakia niccombsii (n=3), Cystobasidium laryngis (n=2), Bannozyma yamatoana (n=2), Holtermanniella nyarrowii (n=2), and Glaciozyma martinii (n=2). This study is the first one reporting extracellular enzyme production by yeasts isolated from thallus of the species of Antarctic lichens Lecania brialmontii, Polycauliona candelaria, Usnea capillacea, Cladonia metacorallifera, and Polycauliona regalis. With these data, it’s possible to confirm lichens as a source of hydrolase-producing yeasts, reinforcing the potential of these microorganisms in bioprospecting studies of catalytic molecules from polar regions that may be useful in promising biotechnological applications.

https://doi.org/10.1590/0001-3765202220210540

Molecular data reveal a complex population genetic structure for Psalidodon scabripinnis (Teleostei: Characidae) in the Atlantic Rainforest, Brazil
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Limeira, Daniel Meneguello

; Santos, Mateus Henrique

; Mateus, Rogério Pincela

; Ruas, Claudete de Fátima

; Almeida, Mara Cristina de

; Moreira Filho, Orlando

; Artoni, Roberto Ferreira

Genetics and Molecular Biology

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2022, Volume 45 Nº 1 elocation e20210048

Resumo: En | Texto: En | PDF: En

Abstract Recently renamed, Psalidodon scabripinnis populations of Serra da Mantiqueira, previously known as Astyanax scabripinnis have been deeply studied in the last years. These populations are small and isolated and occur very close to the watershed between Paraíba do Sul River basin and Upper Paraná River basin, in Serra da Mantiqueira region in the Atlantic Rainforest. These conditions arouse the interest in knowing theor genetic conservation status and how they responded to the separation between the two rivers basins. Therefore, we accessed the genetic diversity of five P. scabripinnis populations of this region with microsatellites and mitochondrial data. The results showed a complex structure pattern that doesn’t match the simple basin separation and a reasonably conservation status when compared with other populations of the same family or with similar natural history.

https://doi.org/10.1590/1678-4685-gmb-2021-0048

Mercados imobiliários e rede urbana no Brasil
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Almeida, Renan P.

; Amano, Fábio Henrique Florindo

; Tupy, Igor Santos

Revista Brasileira de Estudos Urbanos e Regionais

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2022, Volume 24 elocation e202208

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Mercados imobiliários revelam aspectos relativos à rede urbano-regional de um país e também às possibilidades para o desenvolvimento dessa rede de forma policêntrica. Usando uma base de dados inédita, este artigo investiga os mercados imobiliários em metrópoles de segundo, terceiro e quarto níveis no Brasil. Explora-se a variabilidade e criam-se tipologias de mercados imobiliários nas metrópoles selecionadas - Brasília, Belo Horizonte, Salvador, Florianópolis e Vitória - por meio de métodos multivariados. Os resultados indicam altos níveis de segmentação dos mercados, jogam luz sobre os processos de suburbanização em contraposição à presença de centros fortes e geram preocupações sobre a capacidade de pagamento dos residentes em alguns municípios. Dentro de cada metrópole, os mercados imobiliários variam substancialmente, evidenciando alto nível de segregação social; entre elas, há similaridades intrigantes, indicando como os locais residenciais e comerciais são (re)produzidos de maneira relativamente genérica no país.

Abstract Real estate markets reveal aspects regarding the urban-regional network of a country and the possibilities for the development of this network in a polycentric manner. Using an unprecedented database, this article investigates the real estate markets in second-, third- and fourth-tier metropolises in Brazil. It explores the variability and creates typologies of real estate markets in a group of selected metropolises - Brasília, Belo Horizonte, Salvador, Florianópolis and Vitória - using multivariate analysis methods. The results indicate high levels of market segmentation, shed light on the suburbanization processes versus the presence of strong city centers, and raise concerns regarding the affordability of housing in some municipalities. Within each agglomeration, real estate markets vary substantially, indicating a high level of social segregation; among them, there are intriguing similarities, indicating how the residential and commercial locations are (re) produced in a relatively generic manner in Brazil.

https://doi.org/10.22296/2317-1529.rbeur.202208pt

Schools reopening and the COVID-19 pandemic: a case study from Macaé, Rio de Janeiro, Brazil
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POZZOBON, ALLAN P. B.

; PETRY, ANA C.

; ZILBERBERG, CARLA

; BARROS, CINTIA M. DE

; NEPOMUCENO-SILVA, JOSÉ L.

; FEITOSA, NATÁLIA M.

; GOMES NETO, LUPIS R.

; RODRIGUES, BRUNO C.

; BRINDEIRO, RODRIGO M.

; NOCCHI, KEITY JAQUELINE C.V.

; MURY, FLAVIA B.

; SOUZA-MENEZES, JACKSON DE

; SILVA, MANUELA L. DA

; MEDEIROS, MARCIO JOSÉ DE

; GESTINARI, RAQUEL S.

; ALVARENGA, ALESSANDRA S. DE

; SILVA, CARINA A.O.

; SANTOS, DANIELE G. DOS

; SILVESTRE, DIEGO HENRIQUE

; SOUSA, GRAZIELE F. DE

; ALMEIDA, JANIMAYRI F. DE

; SILVA, JHENIFER N. DA

; BRANDÃO, LAYZA M.

; DRUMMOND, LEANDRO O.

; CARPES, RAPHAEL M.

; SANTOS, RENATA C. DOS

; PORTAL, TAYNAN M.

; TANURI, AMILCAR

; NUNES-DA-FONSECA, RODRIGO

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20211361

Resumo: En | Texto: En | PDF: En

Abstract Since the first reported case of COVID-19 in Brazil, the public and private educational system started to close. Up to November 2020, scientific discussions about the return of schooling activities have been rarely performed by the national scientific community and police-makers. The great delay of school returning in Brazil contrasts with successful international strategies of school reopening worldwide and seems counterintuitive with the reopening of non-essential activities. Here, important issues to be considered before and during school reopening are reviewed and discussed. COVID-19 testing is essential to avoid disease spreading, but high cost of individual RT-qPCRs impairs an extensive testing strategy for school returning. To reduce costs and increase the speed of diagnosis, we tested the efficiency of a pooled-sample PCR strategy in a cohort of the educational staff in the city of Macaé/RJ, finding five asymptomatic individuals (0,66%) among the 754 people tested. Thus, a polled-sample PCR testing strategy of the educational staff might prevent infection spreading in schools at a reasonable cost. We discuss how our test strategy could be coupled with internationally recognized safety rules to allow for a safe school return and how countries from different world regions are dealing with educational activities during COVID-19 pandemic.

https://doi.org/10.1590/0001-3765202220211361

10.

HEMATOLOGYCAL AND BLOOD BIOCHEMISTRY PARAMETERS OF CAPTIVE BIG-HEADED AMAZON RIVER TURTLES, Peltocephalus dumerilianus (TESTUDINES: PODOCNEMIDIDAE)
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ANSELMO, Neiana Pereira

; FRANÇA, Mônica Ferreira de Lima

; SANTOS, Mareio Quara de Carvalho

; PANTOJA-LIMA, Jackson

; SILVA, Cleide Karoline Pereira da

; ARIDE, Paulo Henrique Rocha

; OLIVEIRA, Adriano Teixeira de

Acta Biológica Colombiana

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Ago 2021, Volume 26 Nº 2 Páginas 207 - 213

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN La determinación de valores hematológicos se ha utilizado para conocer las condiciones sanitarias de algunas especies animales. La tortuga cabezona del río Amazonas, Peltocephalus dumerilianus, se considera una de las especies de testudines menos conocidas en relación a su biología y estado de salud. Aquí, determinamos los parámetros bioquímicos hematológicos y plasmáticos de 17 adultos (ocho machos y nueve hembras) de P. dumerilianus con el fin de proporcionar valores de intervalo de referencia sobre los individuos clínicamente sanos. Recolectamos las muestras de sangre perforando la vena femoral con jeringas hipodérmicas largas heparinizadas. El dimorfismo sexual de los individuos se determinó mediante la observación externa de la forma del plastrón. Los valores medios obtenidos para los diez parámetros hematológicos y bioquímicos analizados fueron: recuento de glóbulos rojos = 0,32 millones μL-1; hematocrito = 20,6 %; hemoglobina = 8,5 g dL-1; volumen corpuscular medio = 681,6 fL; hemoglobina corpuscular media = 267,8 pg; concentración media de hemoglobina corpuscular = 41,9 g dL-1; glucosa = 80,6 mg dL-1, proteína total = 4,1 g dL-1, triglicéridos = 388,9 mg dL-1 y colesterol total = 79,3 mg dL-1. A pesar del dimorfismo sexual evidente para la especie, no hubo diferencia estadística significativa entre machos y hembras para los parámetros hematológicos y bioquímicos analizados aquí. Con base en estos resultados, la población se considera saludable y los valores de los parámetros coinciden con los rangos de referencia reportados previamente de las especies de testudines en la región. Los resultados obtenidos en este estudio pueden utilizarse en la evaluación del estado de salud de otras poblaciones de tortugas amazónicas, considerando especialmente aquellas acciones dirigidas al manejo, conservación y estrategias de cultivo de la especie.

ABSTRACT The determination of hematological values is used to obtain knowledge about the health conditions of animal species. The big-headed Amazon River turtles, (Peltocephalus dumerilianus) are considered one of the least known testudine species concerning their biology and health status. Herein, we determined the hematological and plasma biochemical parameters of17 (eight males and nine females) adult P. dumerilianus to provide reference interval values for clinically healthy individuals. We collected the blood samples by puncturing the femoral vein using long heparinized hypodermic syringes. Sexual dimorphism for individuals was determined by external observation of the shape of the plastron. The average values obtained for the ten hematological and biochemical parameters analyzed were red blood cell count = 0.32 million μL-1; hematocrit = 20.6 %; hemoglobin = 8.5 g dL-1; mean corpuscular volume = 681.6 fL; mean corpuscular hemoglobin = 267.8 pg; mean corpuscular hemoglobin concentration = 41.9 g dL-1; glucose = 80.6 mg dL-1, total protein = 4.1 g dL-1, triglycerides = 388.9 mg dL-1, and total cholesterol = 79.3 mg dL-1. Despite the sexual dimorphism evidenced for the species, there was no significant statistical difference between males and females for both hematological and biochemical parameters analyzed herein. Based on these results, the population is considered healthy, with parameter values coinciding with previously reported reference ranges for testudines species in the region. The results obtained in this study can be used for assessing the health status of other Amazonian turtle populations, especially in actions aimed at cultivation strategies, management, and species conservation.

https://doi.org/10.15446/abc.v26n2.80616 29 downloads

11.

[SciELO Preprints] - Thickening of aerial roots of Pandanus Parkinson (Pandanaceae) species
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Barbosa, Lucas Henrique Santos Esteves, Marina Cristina Soares Cury-Guapo, Graziela

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Data de publicação: 2021-07-23

Resumo: En Pt

Monocotyledon roots and stems are organs that usually do not show secondary growth or thickness. However, it can occur and for the roots, the literature does not provide much information. Species of the genus Pandanus are easily found in urban areas and are characterized by the presence of thickened aerial roots, originating from the stem. To verify how thickening occurs, roots of P. utilis Bory, P. baptisti Hort., P. amaryllifolius Roxb e P. veitchii Hort samples of the roots were collected in urban areas, fixed and processed according to the usual technique for analysis under a light microscope. For all species, thickening occurs in the acropetal direction, through the formation and expansion of spaces of intercellular spaces in the cortex and in the vascular cylinder, meristematic activity of the proendodermis, division and increase in volume of the parenchymatic cells of the cortex and the vascular cylinder and meristematic activity of the pericycle.

Raízes e caules de monocotiledôneas são órgãos que, usualmente, não apresentam crescimento secundário ou em espessura. Porém, pode ocorrer e, para as raízes, a literatura não fornece muitas informações. Espécies do gênero Pandanus são facilmente encontradas em áreas urbanas e se caracterizam pela presença de raízes aéreas espessadas, oriundas do caule. Para verificar como ocorre o espessamento, raízes de P. utilis Bory, P. baptisti Hort., P. amaryllifolius Roxb e P. veitchii Hort, foram coletadas amostras das raízes em áreas urbanas, fixadas e processadas conforme técnica usual para análise ao microscópio de luz. Para todas as espécies o espessamento ocorre no sentido acrópeto, por meio da formação e expansão de espaços de espaços intercelulares no córtex e no cilindro vascular, atividade meristemática da proendoderme, divisão e aumento de volume das células parenquimáticas do córtex e do cilindro vascular e atividade meristemática do periciclo.

https://doi.org/10.1590/2236-8906-106/2020

12.

Development of a Simple and Fast Procedure Based on Acid Dilution for Determination of Macro and Microelements in Guarana-Based Beverages by ICP OES
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Silva, Vinnícius Henrique C. da

; Silva Jr., André Luiz S. da

; Santos, Herick M.

; Lôbo, Ivon P.

; Bezerra, Marcos A.

; Jesus, Raildo M. de

Journal of the Brazilian Chemical Society

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Jul 2021, Volume 32 Nº 7 Páginas 1467 - 1475

Resumo: En | Texto: En | PDF: En

A simple analytical procedure is proposed for the direct analysis of guarana-based beverages in order to determine nutrient elements by inductively coupled plasma optical emission spectrometry (ICP OES). A Box-Behnken design, combined with Derringer’s desirability function, was applied to the simultaneous optimization of the ICP OES radiofrequency (RF), sample volume and nitric acid concentration. Using this approach, optimal conditions were established as RF power of 1.40 kW, and 5.0 mL of sample diluted twice with 0.5 mol L-1 HNO3. The optimized procedure allowed the determination of Cu, Fe, Mn, Zn, K, Ca, S, P and Mg with limits of quantification of 10.0, 2.0, 0.6, 6.0, 66.0, 65.0, 235, 40.0 and 20.0 µg L-1, respectively. Accuracy of the method was evaluated through analysis of water standard reference material (SRM NIST 1643f) and by addition-recovery experiments, in which satisfactory recoveries were obtained. The procedure was applied for the analysis of guarana-based soft and energy drinks. The concentration ranges (mg L-1) were: 0.36-43.3 (Ca), 4.04-192 (K), 0.36-44.8 (Mg), 10.1-1,073 (S), 0.04-92.4 (P), < 0.010-0.0174 (Cu), < 0.002-0.294 (Fe), < 0.006-0.06 (Zn) and < 0.0006-0.168 (Mn).

https://doi.org/10.21577/0103-5053.20210045 74 downloads

13.

Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates
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Schappo, Ana Paula

; Bittencourt, Najara C

; Bertolla, Leticia P

; Forcellini, Sofia

; da Silva, Ana Beatriz Iung Enembreck

; dos Santos, Hellen Geremias

; Gervásio, João Henrique

; Lacerda, Marcus VG

; Lopes, Stefanie CP

; Costa, Fabio TM

; Albrecht, Letusa

Memórias do Instituto Oswaldo Cruz

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2021, Volume 116 elocation e210227

Resumo: En | Texto: En | PDF: En

BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions. OBJECTIVE To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E. METHODS Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells. FINDINGS Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1. MAIN CONCLUSIONS PvBrVIR-E was antigenic and adhesive to CHO-ICAM1.

https://doi.org/10.1590/0074-02760210227

14.

Advanced Microtexture Evaluation of Dextran Biofilms Obtained from Low Cost Substrate Loaded with Maytenus rigida Extract
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Silva, Mário R. P.

; Matos, Robert S.

; Pinto, Erveton P.

; Santos, Samuel B.

; Monteiro, Michael D. S.

; Fonseca Filho, Henrique D. da

; Almeida, Luiz E.

Materials Research

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2021, Volume 24 Nº 4 elocation e20200597

Resumo: En | Texto: En | PDF: En

Abstract We have obtained kefir biofilms loaded with Maytenus rigida extract. The main goal was to evaluate the influence of the addition of the extract on the biofilms microtexture using stereometric and fractal parameters. Atomic force microscopy, Fourier transform infrared spectroscopy, and contact angle tecniques were used to evaluate the topography, chemical interation and wettability of the biofilms. Chemical evaluation showed that the extract interacts with the polymeric matrix of the biofilms, changing their structure. This behavior affected the surface energy because the wettability decreased when the Maytenus content increased. The surface morphology was also affected and suggested the prevalence of bacteria and yeast at lower and higher extract concentrations, respectively. The surface roughness increased according to the increase in the extract concentration. Texture parameters revealed that the biofilm with lowest concentration had deeper furrows than for higher concentration. Advanced fractal parameters revealed that topographic uniformity, percolation and texture homogeneity were more suitable for the biofilm with 0.25 g.L-1 of extract. These results indicate that this biofilm have promising 3D micromorphology for regenerative medicine application. Furthermore, the physics tools used revealed to be very useful for characterizing of the microtexture of polymeric-based biological materials.

https://doi.org/10.1590/1980-5373-mr-2020-0597 122 downloads

15.

Espessamento das raízes aéreas de espécies de Pandanus Parkinson (Pandanaceae)
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Barbosa, Lucas Henrique Santos

; Esteves, Marina Cristina Soares

; Cury-Guapo, Graziela

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2021, Volume 48 elocation e1062020

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO (Espessamento das raízes aéreas de espécies de Pandanus Parkinson (Pandanaceae)). Raízes e caules de monocotiledôneas são órgãos que, usualmente, não apresentam crescimento secundário ou em espessura. Porém, pode ocorrer e, para as raízes, a literatura não fornece muitas informações. Espécies do gênero Pandanus são facilmente encontradas em áreas urbanas e se caracterizam pela presença de raízes aéreas espessadas, oriundas do caule. Para verificar como ocorre o espessamento, raízes de P. utilis Bory, P. baptisti Hort., P. amaryllifolius Roxb e P. veitchii Hort, foram coletadas amostras das raízes em áreas urbanas, fixadas e processadas conforme técnica usual para análise ao microscópio de luz. Para todas as espécies o espessamento ocorre no sentido acrópeto, por meio da formação e expansão de espaços de espaços intercelulares no córtex e no cilindro vascular, atividade meristemática da proendoderme, divisão e aumento de volume das células parenquimáticas do córtex e do cilindro vascular e atividade meristemática do periciclo.

ABSTRACT Monocotyledon roots and stems are organs that usually do not show secondary growth or thickness. However, it can occur and for the roots, the literature does not provide much information. Species of the genus Pandanus are easily found in urban areas and are characterized by the presence of thickened aerial roots, originating from the stem. To verify how thickening occurs, roots of P. utilis Bory, P. baptisti Hort., P. amaryllifolius Roxb e P. veitchii Hort samples of the roots were collected in urban areas, fixed and processed according to the usual technique for analysis under a light microscope. For all species, thickening occurs in the acropetal direction, through the formation and expansion of spaces of intercellular spaces in the cortex and in the vascular cylinder, meristematic activity of the proendodermis, division and increase in volume of the parenchymatic cells of the cortex and the vascular cylinder and meristematic activity of the pericycle.

https://doi.org/10.1590/2236-8906-106/2020 1 downloads

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