ABSTRACT Objective: To investigate whether the dimensions of cavitated dentin carious lesions on the occlusoproximal surfaces of primary teeth could predict the location of cement-enamel junction (CEJ). Material and Methods: Two hundred extracted primary molars were selected and digital images were obtained. The teeth were set in arch models for clinical measurement. The cervical-occlusal (CO) and buccal-lingual/palatal (BL/P) cavities’ dimensions were obtained by digital (Image J) and clinical (periodontal millimeter probe) assessments. The cervical margin location was also determined. The thresholds (cut-off points) were determined by sensitivity, specificity and the areas under the receiver operating characteristics curves (Az) for the two methods. Pearson's correlation coefficient was used to investigate the correlation between clinical and digital measurements. Logistic regression analysis was performed to evaluate the association between the dimensions and cervical margin location. Results: There was a strong correlation between methods for all measurements (CO: r=0.90, VL/P: r=0.95). Cavities with BL/P distance higher than 4.5 mm and CO dimension higher than 3.5 mm had a lower chance of presenting the cervical limit above the CEJ, irrespective of the measurement method. Conclusion: CO and VL/P dimensions could be used to predict the CEJ location and, ultimately, as a clinical parameter for restorative decision-making.

Objective: To assess differences in blood inflammatory cytokines between people with alcohol use disorder (AUD) and healthy controls (HC). Methods: Searches were performed from inception through April 14, 2021. Meta-analyses with random-effects models were used to calculate the standardized mean difference ([SMD], 95%CI), and potential sources of heterogeneity were explored trough meta-regressions and subgroup analysis. Results: The meta-analysis included 23 studies on the following 14 cytokines: tumor necrosis factor (TNF)-α, IL-1, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, IL15, interferon (IFN)-γ and sCD14. There were significantly higher concentrations of IL-6 (n=462 AUD and 408 HC; SMD = 0.523; 95%CI 0.136-0.909; p = 0.008) in AUD than HC. No significant differences were found in the other 13 cytokines. Conclusion: We found that IL-6 levels were significantly higher in individuals with AUD than HC and that other cytokines were not altered. This can be explained by the small number of studies, their methodological heterogeneity, and confounding factors (active use, abstinence, quantity, and physical or psychiatric illnesses, for example). Despite a great deal of evidence about alcohol and inflammatory diseases, studies assessing the role of neuroimmune signaling in the development and severity of AUD are still lacking.

Abstract Objective: This study aims to describe the epidemiological characteristics and survival rates of children with acute myeloid leukemia treated in hospitals in southern Brazil and compare them with international data. Methods: A multicenter cohort study was conducted with retrospective data collection of all new patients with acute myeloid leukemia under 18 treated at five referral centers in pediatric hematology-oncology in southern Brazil between January 2005 and December 2015. Results: Of the 149 patients with acute myeloid leukemia, 63.0% (n = 94) were male. The median age at diagnosis was 10.5 years (range 0–18 years) and 40.3% (n = 60) had a white blood cell count below 50,000/mm2. The most common Franco-American-British (FAB) subtype was M3 (n = 43, 28.9%). Nine (6.0%) patients had central nervous system disease. In M3 patients, overall survival (OS) was 69.2% and 3-year event-free survival was 67.7%; in non-M3 patients, these rates were 45.3% and 36.7%, respectively. In non-M3 patients, OS was significantly different between transplanted (61.8%) and non-transplanted (38.2%) patients (p = 0.031). Conclusions: These results show a higher prevalence of the Franco-American-British M3 subtype than that reported in the international literature, as well as a decreased OS compared with that of developed countries. Further multicenter Brazilian studies with a larger sample size are encouraged to better understand the characteristics of acute myeloid leukemia, and to improve the treatment and prognosis in this population.

Abstract Introduction The dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis has a key role in drug addiction susceptibility. In addition to the well-known relationship between cortisol and the HPA axis, other molecules are involved with stress response and could modify the HPA activation, such as the neuropeptide Y (NPY), which has anxiolytic proprieties. There are few studies evaluating the effect of NPY levels on addiction, especially in crack cocaine dependence. Objective To evaluate NPY in crack users during early withdrawal to determine its relationship with drug use and cortisol levels. Methods We analyzed 25 male inpatient crack users. Serum NPY levels were measured at admission and discharge (mean of 24 days). Morning salivary cortisol was measured at admission. Results Serum NPY levels at admission and discharge were very similar. Lower NPY levels at discharge were associated with higher lifetime crack use. Also, a negative correlation was found between morning cortisol and delta NPY (NPY discharge – NPY admission). Conclusion These preliminary findings indicate that crack use influences the modulation of NPY levels and modifies stress response. The NPY pathway may play an important role in the pathophysiology of crack addiction, and the anxiolytic effect of NPY may be impaired in crack users. Future studies should consider NPY as a measurable indicator of the biological state in addiction.

RESUMEN Objetivo Evaluar la ganancia de peso durante el embarazo según el estado pregestacional en mujeres que se sometieron a atención prenatal en Atención Primaria de Salud. Métodos Se hizo un estudio transversal con la participación de las mujeres embarazadas. Las variables socioeconómicas y las variables demográficas se utilizaron a un cuestionario cuestionable. Las mujeres fueron evaluadas para el estado nutricional y la dieta. La relación con la edad de la mujer embarazada, la semana de gestación, obesidad, peso actual, peso previo a la gestación se obtuvieron de la forma prenatal. El Statistical Package para las Ciencias Sociales (SPSS) fue utilizado para el análisis estadístico. Resultados La evaluación del estado nutricional previo a la gestación reveló que el 43,2% (n=110) de las mujeres comenzaron su gestación con sobrepeso y el 4,3%, con un peso muy bajo. El 51% (n=130) obtuvo un peso gestacional por encima del recomendado. La edad media de las mujeres con un IMC previo a la gestación mayor o igual a 25 kg/m2 fue significativamente más alto que la de las mujeres con un BMI inferior a 25 km/ m2. La ingesta de energía total (p=0,037) y el calcio (p=0,004) fue mayor en las mujeres que subieron peso por encima del recomendado. Discusión Los resultados obtenidos resaltan la importancia de las estrategias de salud pública para evitar la ganancia de peso durante el embarazo. Conclusión Las mujeres embarazadas aumentan de peso por encima del valor máximo recomendados, acorde con el IMC previo a la gestación. Este hecho provoca consecuencias negativas en materia de maternidad e infancia.

ABSTRACT Objective To evaluate weight gain during pregnancy according to the pregestational state in women who underwent prenatal care in Primary Health Care. Methods A cross-sectional study with the participation of 255 pregnant women. Socioeconomic and demographic variables were collected using a structured questionnaire. Women were evaluated for nutritional status and dietary intake. Data related to the age of the pregnant woman, gestational week, current weight, pregestational weight, and height were obtained from the prenatal follow-up form. The Statistical Package for the Social Sciences (SPSS) was used for statistical analysis. Results Pregestational nutritional status assessment showed that 43.2% (n=110) of the women started gestation with overweight and 4.3% (n=11) started with low weight. 51% percent (n=130) gained gestational weight above the recommended level. The mean age of women with pregestational BMI ≥ 25 kg/m2 was significantly higher than that of those with BMI <25 kg/rrP (p<0.001). Total energy (p=0.037) and calcium (p=0.004) intake were higher in women with weight gain above the recommended. Discussion The results presented highlight the importance of strategies in public health to avoid excess weight gain during pregnancy. Conclusion Pregnant women presented a gestational weight gain above the recommended maximum value according to pregestational BMI, which may contribute to adverse maternal and infant outcomes.

ABSTRACT Objective To evaluate weight gain during pregnancy according to the pregestational state in women who underwent prenatal care in Primary Health Care. Methods A cross-sectional study with the participation of 255 pregnant women. Socioeconomic and demographic variables were collected using a structured questionnaire. Women were evaluated for nutritional status and dietary intake. Data related to the age of the pregnant woman, gestational week, current weight, pregestational weight, and height were obtained from the prenatal follow-up form. The Statistical Package for the Social Sciences (SPSS) was used for statistical analysis. Results Pregestational nutritional status assessment showed that 43.2% (n=110) of the women started gestation with overweight and 4.3% (n=11) started with low weight. 51% percent (n=130) gained gestational weight above the recommended level. The mean age of women with pregestational BMI ≥ 25 kg/m2 was significantly higher than that of those with BMI <25 kg/rrP (p<0.001). Total energy (p=0.037) and calcium (p=0.004) intake were higher in women with weight gain above the recommended. Discussion The results presented highlight the importance of strategies in public health to avoid excess weight gain during pregnancy. Conclusion Pregnant women presented a gestational weight gain above the recommended maximum value according to pregestational BMI, which may contribute to adverse maternal and infant outcomes.

RESUMEN Objetivo Evaluar la ganancia de peso durante el embarazo según el estado pregestacional en mujeres que se sometieron a atención prenatal en Atención Primaria de Salud. Métodos Se hizo un estudio transversal con la participación de las mujeres embarazadas. Las variables socioeconómicas y las variables demográficas se utilizaron a un cuestionario cuestionable. Las mujeres fueron evaluadas para el estado nutricional y la dieta. La relación con la edad de la mujer embarazada, la semana de gestación, obesidad, peso actual, peso previo a la gestación se obtuvieron de la forma prenatal. El Statistical Package para las Ciencias Sociales (SPSS) fue utilizado para el análisis estadístico. Resultados La evaluación del estado nutricional previo a la gestación reveló que el 43,2% (n=110) de las mujeres comenzaron su gestación con sobrepeso y el 4,3%, con un peso muy bajo. El 51% (n=130) obtuvo un peso gestacional por encima del recomendado. La edad media de las mujeres con un IMC previo a la gestación mayor o igual a 25 kg/m2 fue significativamente más alto que la de las mujeres con un BMI inferior a 25 km/ m2. La ingesta de energía total (p=0,037) y el calcio (p=0,004) fue mayor en las mujeres que subieron peso por encima del recomendado. Discusión Los resultados obtenidos resaltan la importancia de las estrategias de salud pública para evitar la ganancia de peso durante el embarazo. Conclusión Las mujeres embarazadas aumentan de peso por encima del valor máximo recomendados, acorde con el IMC previo a la gestación. Este hecho provoca consecuencias negativas en materia de maternidad e infancia.

RESUMO A pecuária brasileira está em franca expansão, porém associada ao uso de medicamentos veterinários que podem ser considerados contaminantes emergentes. Este estudo objetivou caracterizar o descarte dos Resíduos de Serviços de Saúde Animal (RSSA), especialmente dos medicamentos veterinários, além de identificar os medicamentos mais usados no município de São Joaquim, Sul do Brasil. Foram entrevistados 84 criadores de gado por meio de questionário estruturado com questões fechadas sobre uso de medicamentos e descarte dos RSSA. Os princípios ativos de medicamentos mais usados foram o antiparasitário ivermectina (68% das propriedades) e o antimicrobiano oxitetraciclina (48%). Após o uso, o descarte dos RSSA foi no resíduo domiciliar, queimado e a depositado no solo. O teste Qui quadrado mostrou associação entre a idade dos participantes e o descarte de perfurocortantes (X2 = 36,36, p= 0,020), evidenciando que agricultores com idade acima de 60 anos costumam reutilizar, aqueles com idade abaixo de 20 anos devolvem no local onde adquirem o material e os demais adotam práticas inadequadas para o descarte. As práticas de descarte dos RSSA adotadas nas propriedades rurais estão em desacordo com a legislação brasileira vigente e podem ocasionar efeitos adversos na saúde humana, animal e ambiental.

ABSTRACT Brazilian animal livestock is in full expansion but linked to the use of veterinary drugs which may be considered as emerging contaminants. The study aimed to characterize the disposal of Animal Healthcare Service Waste (AHSW), especially of veterinary drugs, besides identifying the most used drugs in the city of São Joaquim municipality, southern Brazil. Eighty-four cattle ranchers were interviewed through a structured questionnaire with closed questions about drugs use and disposal of the AHSW. The active ingredients of drugs most used were the antiparasitic ivermectin (68% of properties) and the antimicrobial oxytetracycline (48%). After the use, the disposal of the AHSW, including expired drugs, leftovers and wrapper, was the disposal next to the domestic trash, burned and deposited in the soil. The Chi square test showed association between the age of the participants and the disposal of sharp objects (X2 = 36,36, p= 0,020), showing that farmers aged above 60 years usually reuse, the ones who are 20 years old return to the place where they acquired the material and the rest adopts improper practices. The disposal practices of the AHSW adopted in the properties are still in disagreement with the current Brazilian legislation and may cause adverse effects on human, animal and environmental health.

RESUMO A pecuária brasileira está em franca expansão, porém associada ao uso de medicamentos veterinários que podem ser considerados contaminantes emergentes. Este estudo objetivou caracterizar o descarte dos Resíduos de Serviços de Saúde Animal (RSSA), especialmente dos medicamentos veterinários, além de identificar os medicamentos mais usados no município de São Joaquim, Sul do Brasil. Foram entrevistados 84 criadores de gado por meio de questionário estruturado com questões fechadas sobre uso de medicamentos e descarte dos RSSA. Os princípios ativos de medicamentos mais usados foram o antiparasitário ivermectina (68% das propriedades) e o antimicrobiano oxitetraciclina (48%). Após o uso, o descarte dos RSSA foi no resíduo domiciliar, queimado e a depositado no solo. O teste Qui quadrado mostrou associação entre a idade dos participantes e o descarte de perfurocortantes (X2 = 36,36, p= 0,020), evidenciando que agricultores com idade acima de 60 anos costumam reutilizar, aqueles com idade abaixo de 20 anos devolvem no local onde adquirem o material e os demais adotam práticas inadequadas para o descarte. As práticas de descarte dos RSSA adotadas nas propriedades rurais estão em desacordo com a legislação brasileira vigente e podem ocasionar efeitos adversos na saúde humana, animal e ambiental.

ABSTRACT Brazilian animal livestock is in full expansion but linked to the use of veterinary drugs which may be considered as emerging contaminants. The study aimed to characterize the disposal of Animal Healthcare Service Waste (AHSW), especially of veterinary drugs, besides identifying the most used drugs in the city of São Joaquim municipality, southern Brazil. Eighty-four cattle ranchers were interviewed through a structured questionnaire with closed questions about drugs use and disposal of the AHSW. The active ingredients of drugs most used were the antiparasitic ivermectin (68% of properties) and the antimicrobial oxytetracycline (48%). After the use, the disposal of the AHSW, including expired drugs, leftovers and wrapper, was the disposal next to the domestic trash, burned and deposited in the soil. The Chi square test showed association between the age of the participants and the disposal of sharp objects (X2 = 36,36, p= 0,020), showing that farmers aged above 60 years usually reuse, the ones who are 20 years old return to the place where they acquired the material and the rest adopts improper practices. The disposal practices of the AHSW adopted in the properties are still in disagreement with the current Brazilian legislation and may cause adverse effects on human, animal and environmental health.

Resumo O conjunto de paredes e portas separadas pela circulação de uso comum em edifícios residenciais passou a ter seu desempenho acústico avaliado, a partir da entrada em vigor da norma brasileira NBR 15575. Nesse contexto, este trabalho propõe determinar a influência das características dos elementos construtivos - portas e circulações entre unidades habitacionais - no isolamento ao ruído aéreo do conjunto de paredes e portas separadas pelo hall de entrada. Foram escolhidos três prédios e realizada uma série de ensaios de desempenho acústico em campo, abrangendo diversas situações. Também foram usados artifícios para a vedação das frestas das portas, a fim de verificar a melhora do desempenho do sistema. Os resultados obtidos apontam para a perda de desempenho acústico com o aumento das frestas entre a folha da porta e o piso. Ainda, foram encontradas relações entre o tempo de reverberação dos ambientes de circulação e a distância entre as portas de entrada das unidades habitacionais. Pode-se concluir que o alinhamento frontal de portas de entrada das unidades habitacionais distintas e halls com elevado tempo de reverberação reduzem a eficiência do isolamento acústico do conjunto de portas e parede voltadas para circulações de uso comum em edifícios habitacionais, assim como frestas mal vedadas.

Abstract The set of walls and doors separated by communal circulation areas in residential buildings began to have their acoustic performance evaluated since the Brazilian standard NBR 15575 was implemented. The aim of this paper is to determine the influence of the characteristics of the constructive elements - doors and corridors between housing units - on the insulation of the air noise of the set of walls and doors separated by the entrance hall. Three buildings were chosen and a series of acoustic performance tests were performed on the site, covering several situations. Devices were used to seal the door gaps in order to verify the improvement of the performance of the system. The results point to loss of acoustic performance with larger gaps between the door and the floor. In addition, a relationship was detected between the reverberation time of the circulation environments and the distance between the entrance doors of the housing units. The conclusion of the study is that frontal alignment of the entrance doors of the different housing units and corridors with high reverberation time as well as badly sealed gaps reduce the acoustic insulation efficiency of the set of doors and wall facing communal circulation areas in residential buildings.

Resumo Objetivos: relacionar o estado nutricional pré-gestacional, a idade materna e o número de gestações com a distribuição de macronutrientes e micronutrientes conforme o tipo de processamento dos alimentos consumidos por gestantes de alto risco. Métodos: estudo retrospectivo transversal, realizado a partir de dados de prontuários de 200 gestantes atendidas em um ambulatório público do Rio Grande do Sul, no período de 2014 a 2016. Resultados: a média de consumo em percentuais de lipídios, ácidos graxos monoinsaturados, poli-insaturados e sódio foi maior entre os alimentos ultraprocessados. Observou-se correlação significativamente inversa entre a idade materna e o consumo de calorias totais (p=0,003), percentuais de carboidratos (p=0,005) e proteínas (p=0,037) provenientes de alimentos ultraprocessados. Verificou-se também associação significativa entre o estado nutricional pré-gestacional e o consumo de calorias totais (p=0,018) e percentual de carboidrato (p=0,048) provenientes de alimentos ultraprocessados. Conclusões: a média de consumo em percentuais de lipídios, ácidos graxos monoinsaturados, poli-insaturados e sódio foi maior entre os alimentos ultraprocessados, verificou-se que quanto maior a idade materna da gestante de alto risco, menor é o consumo de calorias totais, percentuais de carboidratos e proteínas, oriundos dos alimentos ultraprocessados e identificou-se também que o estado nutricional pré-gestacional possui associação significativa com o consumo de calorias totais e percentual de carboidrato provenientes de alimentos ultraprocessados.

Abstract Objectives: to relate pregestational nutritional status, maternal age and number of pregnancies to the distribution of macronutrients and micronutrients according to the type of processing offoods consumed by high-risk pregnant women. Methods: a retrospective cross-sectional study was carried out with data from medical records of 200 pregnant women served by a public outpatient clinic in Rio Grande do Sul from 2014 to 2016. Results: the mean percentages of lipids, monounsaturated fatty acids, polyunsaturated fatty acids and sodium intake were higher among ultra-processed foods. There was a significant inverse correlation between maternal age and total calorie intake (p=0.003) and percentage of carbohydrates (p=0.005) and proteins (p=0.037) from ultra-processed foods. There was also a significant association between pregestational nutritional status and total calorie intake (p=0.018) and percentage of carbohydrates (p=0.048) from ultra-processed foods. Conclusions: the mean percentages of lipids, monounsaturated fatty acids, polyunsaturated fatty acids and sodium intake were higher among ultra-processed foods. It was observed that the older the maternal age of high-risk pregnant women, the lower the intake of total calories and percentages of carbohydrates and proteins from ultra-processed foods. It was also observed that pregestational nutritional status was significantly associated with the intake of total calories and percentage of carbohydrates from ultra-processed foods.

RESUMO: Infecções por Staphyloccocus pseudintermedius (SP) na pele de cães tem sido objeto de estudos, uma vez que esses microrganismos geralmente apresentam resistência múltipla à antibióticos. Este estudo teve como objetivo identificar e avaliar a susceptibilidade antimicrobiana das cepas de SP isoladas de cães com otite. Amostras de ambas orelhas de 52 cães com otite (n=104) foram incluídas. As bactérias foram cultivadas em ágar Muller-Hinton suplementado com 5% de sangue equino e incubadas a 37ºC por 24 a 48h. Todas as colônias foram submetidas à avaliação bioquímica para identificação de estafilococos. A identificação das colônias como SP foi confirmada pela reação em cadeia da polimerase. A susceptibilidade antimicrobiana das cepas SP foi avaliada pela técnica de difusão em disco. A presença do gene mecA foi avaliada em todos os isolados de SP por PCR. 44 estirpes de SP foram isoladas de 31 cães (31/52, 59,6%). 75% das cepas foram suscetíveis a cefalexina e 93,2% à amoxicilina mais ácido clavulânico. Menos de 23% das estirpes eram suscetíveis à penicilina. Para antimicrobianos não beta-lactâmicos, 63,6% apresentaram resistência ao sulfametoxazol-trimetoprim, 61,4% à tetraciclina e 38,64% à enrofloxacina. A frequência de resistência a aminoglicosídeos foi de 27,3% para gentamicina. A resistência a oxacilina in vitro foi detectada em 13 das 44 estirpes (29,55%). Um total de 12 amostras (27,3%) foram positivas para mecA, cinco das quais foram suscetíveis à oxacilina na difusão em disco. 26 amostras (59,1%) foram resistentes a três ou mais classes de antimicrobianos e classificadas como multirresistentes. Nossos resultados mostram elevada frequência de isolados SP e multiresistentes para os antimicrobianos comumente usados em veterinária.

ABSTRACT: Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis. Swabs from both ears of 52 dogs with otitis (n=104) were included. Bacteria were cultured using Muller-Hinton agar (supplemented with 5% equine blood and incubated at 37°C for 24 to 48 hours. All colonies underwent biochemical evaluation for identification of staphylococci. The identity of colonies as SP was confirmed by polymerase chain reaction. The antimicrobial susceptibility of SP strains was evaluated by disk diffusion. The presence of the gene mecA was evaluated in all SP isolates by PCR. Forty-four SP strains were isolated from swabs of 31 dogs (31/52, 59.6%). Seventy-five percent of the strains were susceptible to cephalexin and 93.2% to amoxicillin plus clavulanic acid. Less than 23% of the strains were susceptible to penicillin. For non-beta-lactam antimicrobials, 63.6% of the strains showed resistance to sulfamethoxazole-trimethoprim, 61.4% to tetracycline, and 38, 64% to enrofloxacin. Aminoglycoside resistance rate was 27.3% for gentamicin. Resistance to oxacillin in vitro was detected in 13 of the 44 strains (29, 55%). A total of 12 strains (27.3%) were positive for mecA gene and five of these 12 strains were susceptible to in vitro oxacillin. Twenty-six (59, 1%) strains were resistant to three or more classes of antimicrobials, and classified as multi resistant. Our results showed high frequency of SP and multi resistant isolates to antimicrobials commonly used in veterinary.

RESUMO: Para acessar a prevalência de MRSP na pele e nas narinas de cães com piodermite superficial, determinar a suscetibilidade in vitro, e correlacionar estes dados com a presença do gene mecA, foram selecionados 43 cães. Amostras de lesões de pele e narinas foram coletadas, cultivadas, e analisadas fenotipica e genotipicamente. Em 62 amostras (91%), os microrganismos foram classificados como Staphylococcus pseudintermedius. A taxa de resistência a antibióticos variou entre 7% (amicacina; 4/62) e 77% (sulfamethoxazol + trimetoprim; 48/62). Resistência a oxacilina foi observada em 34% das amostras (21/62). Vinte e cinco amostras (37%), eram cepas portadoras do gene mecA. Correlação significativa (P<0,01) foi observada entre a presença do gene mecA e a resistência à oxacilina. Considerando os cães, 17 eram portadores de cepas com gene mecA e 8(47%) delas carreavam este gene nas amostras de lesão de pele e nas narinas. Correlação significativa (P>0,01) foi observada entre a presença do gene mecA nas lesões de pele e nas narinas. Sendo assim, resistência à oxacilina in vitro pode ser aferida com segurança para indicar a presença do gene mecA em amostras de MRSP, e as narinas podem constituir em um reservatório dos microorganismos em cães.

ABSTRACT: In order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs.

Resumo Objetivo: Comparar a sensibilidade gustativa de indivíduos adultos e idosos e relacionar com o estado nutricional, tabagismo e consumo de álcool. Método: Participaram da pesquisa 46 voluntários, sendo 13 adultos funcionários de uma empresa do ramo de alimentação e 33 idosos residentes em três Instituições de Longa Permanência para Idosos do Vale do Taquari. Os mesmos foram submetidos a um questionário estruturado sobre dados socioeconômico e utilização de fármacos, tabagismo e consumo de álcool, avaliação nutricional e teste de sensibilidade gustativa. O teste de sensibilidade gustativa foi aplicado em três concentrações diferentes para cada sabor, sendo pingadas 4 gotas na língua do voluntário que referiu qual foi o grau de palatabilidade percebido, atribuindo um conceito de 0 a 5. Os dados foram analisados através do Pacote Estatístico SPSS 20.0, sendo considerados significativos valores de p<0,05. Resultado: Observou-se redução na sensibilidade gustativa dos idosos na solução azeda e doce, quando comparados aos adultos, visto que a maioria dos idosos estabeleceu conceitos menores na escala de sensibilidade para a solução de ácido cítrico na concentração 1 (p=0,004), na concentração 2 (p=0,049) e de sacarose na concentração 3 (p=0,026). Conclusão: Os idosos tiveram a percepção do sabor azedo e doce reduzida quando comparado aos adultos. Além disso, o estado nutricional, gênero, consumo de álcool, tabagismo e uso de medicações não tiveram associação significativa com a percepção do paladar nos indivíduos avaliados.

Abstract Objective: To compare the taste sensitivity of adults and elderly people, considering nutritional status, smoking and alcohol consumption. Method: Forty-six volunteers participated in the research, thirteen of whom were adult employees of a company from the food industry and thirty-three of whom were elderly residents of three Long-Term Care Facilities for the Elderly from the Vale do Taquari (Taquari Valley). They responded to a structured questionnaire about their socioeconomic data and use of medication, smoking and alcohol consumption, and underwent a nutritional evaluation and a taste sensitivity test. The taste sensitivity test was applied at three different concentrations for every flavor, with 4 drops dripped on the tongue of the volunteer, who described the level of perceived palatability, giving a score of 0-5. Data was analyzed through statistical analysis using the Statistical Package SPSS 20.0, considering a value of significance of p<0.05. Result: a reduction in the taste sensitivity of the elderly persons was noted for the sweet and sour solutions, in comparison with adults, as the majority of the elderly people demonstrated lower sensitivity scale scores for the citric acid solution in concentration 1 (p=0.004) and concentration 2 (p=0.049) and sucrose in concentration 3 (p=0.026). Conclusion: Elderly people had a lower perception of the sweet and sour flavors than adults. Moreover, nutritional status, gender, alcohol consumption, smoking and medication use were not significantly associated with the taste perception of the individuals evaluated.

ABSTRACT Uliginosin B, a phloroglucinol isolated from Hypericum polyanthemum Klotzsch ex Reichardt, Hypericaceae, has antidepressant-like effect in the forced swimming test in rodents and inhibits monoamines neuronal reuptake without binding to their neuronal carriers. Studies showed the involvement of Na+,K+-ATPase brain activity in depressive disorders, as well as the dependence of neuronal monoamine transport from Na+ gradient generated by Na+,K+-ATPase. This study aimed at evaluating the effect of uliginosin B on Na+,K+-ATPase activity in mice cerebral cortex and hippocampus (1 and 3 h after the last administration) as well as the influence of veratrine, a Na+ channel opener, on the antidepressant-like effect of uliginosin B. Mice were treated (p.o.) with uliginosin B single (10 mg/kg) or repeated doses (10 mg/kg/day, 3 days). Acute administration reduced the immobility in the forced swimming test and tail suspension test and increased Na+,K+-ATPase activity in cerebral cortex 1 h after treating, whereas the repeated treatment induced the antidepressant-like effect and increased the Na+,K+-ATPase activity at both times evaluated. None treatment affected the hippocampus enzyme activity. Veratrine pretreatment prevented uliginosin B antidepressant-like effect in the forced swimming test, suggesting the involvement of Na+ balance regulation on this effect. Altogether, these data indicate that uliginosin B reduces the monoamine uptake by altering Na+ gradient.