Resumo A cultura sanguínea de rotina é usada para a detecção de infecções na corrente sanguínea por bactérias aeróbias e anaeróbias e por leveduras patogênicas comuns. Estudo retrospectivo realizado em hospital público de Maceió-AL, por meio da coleta de dados de todos os prontuários com culturas sanguíneas positivas. Das 2.107 culturas sanguíneas realizadas, 17% foram positivas com Staphylococcus coagulase negativo (51,14%), seguido por Staphylococcus aureus (11,21%) e Klebsiella pneumoniae (6,32%). As bactérias Gram-positiva predominaram entre as culturas de sangue positivas, destacando-se o grupo das Staphylococcus coagulase-negativo. Enquanto as bactérias Gram-negativas apresentaram um número maior de espécies entre as culturas de sangue positivas.

Abstract Routine blood culture is used for the detection of bloodstream infections by aerobic and anaerobic bacteria and by common pathogenic yeasts. A retrospective study was conducted in a public hospital in Maceió-AL, by collecting data of all medical records with positive blood cultures. Out of the 2,107 blood cultures performed, 17% were positive with Staphylococcus coagulase negative (51.14%), followed by Staphylococcus aureus (11.21%) and Klebsiella pneumoniae (6.32%). Gram-positive bacteria predominated among positive blood cultures, highlighting the group of Staphylococcus coagulase-negative. While Gram-negative bacteria had a higher number of species among positive blood cultures.

ABSTRACT Brackish waters has been increasingly used in hydroponic systems for the cultivation of vegetables. However, its use can cause significant losses in crop production. Therefore, new alternatives to enhance the tolerance of plants to salt stress are being studied, including seed priming with hydrogen peroxide (H2O2). Thus, this study aimed to assess the seed priming with H2O2 at different periods of exposure for enhancing the production, water status and pigments concentration of crisp lettuce grown under salt stress. The experiment was carried out under protected conditions, in a completely randomized design, with four replicates. The plants were cultivated in a floating hydroponic system, containing nutrient solution. Five treatments were tested: control (absence of H2O2 and absence of NaCl); salt control (absence of H2O2 and presence of 100 mM NaCl); 0.1 mM H2O2 (12 hours) + 100 mM NaCl; 0.1 mM H2O2 (24 hours) + 100 mM NaCl, and 0.1 mM H2O2 (36 hours) + 100 mM NaCl. In general, salinity reduced the height, production of the fresh and dry mass of the shoot, relative water content, and chlorophylls concentration of lettuce plants. However, the application of 0.1 mM H2O2 for 12 and 36 hours on the seeds, enhanced the growth, water status, and chlorophylls concentration of the plants. Seed priming with H2O2 at a 0.1 mM concentration for 12 hours can be recommended to increase tolerance of lettuce plants grown in a hydroponic system under salt stress.

RESUMO As águas salobras vêm sendo cada vez mais utilizadas em sistemas hidropônicos para o cultivo de hortaliças. Entretanto, seu uso pode provocar prejuízos significativos na produção das culturas. Assim, este trabalho teve como objetivo avaliar o condicionamento fisiológico de sementes com H2O2 em diferentes períodos de exposição para melhorar a produção, estado hídrico e teor de pigmentos de alface crespa cultivada sob estresse salino. O experimento foi conduzido em condições protegidas, em delineamento inteiramente casualizado, com quatro repetições. As plantas foram cultivadas em sistema hidropônico do tipo floating, contendo solução nutritiva de Furlani. Foram testados cinco tratamentos: controle (ausência de H2O2 e ausência de NaCl); controle salino (ausência de H2O2 e presença de 100 mM NaCl); 0,1 mM H2O2 (12 horas) + 100 mM NaCl; 0,1 mM H2O2 (24 horas) + 100 mM NaCl e 0,1 mM H2O2 (36 horas) + 100 mM NaCl. Em geral, a salinidade reduziu a altura, a produção de massa fresca e seca da parte aérea, o teor relativo de água e o teor de clorofilas das plantas de alface. No entanto, a aplicação de 0,1 mM de H2O2 por 12 e 36 horas na semente melhorou o crescimento, o estado hídrico e o teor de clorofila das plantas. O condicionamento de sementes com H2O2 na concentração de 0,1 mM por 12 horas pode ser recomendado para aumentar a tolerância de plantas de alface cultivadas em sistema hidropônico sob estresse salino.

ABSTRACT The production of seedlings of forest species for reforestation of biomes is a practice that restores the conditions of the environment, but water and substrates that favor the growth of seedlings are required. However, in the semi-arid region, water is a limiting factor, requiring the use of alternative sources such as treated domestic sewage. In this context, the objective was to evaluate the growth of Anadenanthera colubrina L. seedlings produced in substrates (soil + bovine manure and soil + coconut fiber) fertilized with treated domestic sewage effluent diluted in public-supply water in proportions (100, 75, 50 and 25% of domestic effluent) and using water from the supply network as a control. The treatments were arranged in a completely randomized design, in a split-plot with three replicates. The treated domestic effluent (TDE) used in the experiment came from the domestic sewage treatment plant of the decanter-digester type of the Projeto de Assentamento Milagre Apodi/RN. The study was conducted in a seedling production nursery at UFERSA, with growth and vigor evaluations at 30, 60, 90 and 120 days after transplanting. The production of seedlings fertigated with domestic effluent caused differences in all growth variables, with improvement in phytomass production. Seedlings fertigated with a dilution of 50% TDE and 50% water supply showed better results when produced with substrate composed of soil and coconut fiber. The seedlings showed good quality, and those cultivated with 50% and 75% of wastewater showed better quality after 120 days of cultivation.

RESUMO A produção de mudas de espécies florestais para reflorestamento de biomas é uma prática que restaura as condições do meio, porém é necessário água e substratos que favoreçam o crescimento das mudas. Entretanto, na região semiárida a água é um fator limitante sendo necessário uso de fontes alternativas como os esgotos domésticos tratados. Neste sentido, objetivou-se avaliar o crescimento de mudas de angico produzidas em substratos (solo + esterco bovino e solo + fibra de coco) fertigadas com efluente de Esgoto Doméstico Tratado (EDT) e diluídos em água de abastecimento nas proporções (100, 75, 50 e 25% do EDT) e água de abastecimento (testemunha). Os tratamentos foram dispostos em delineamento inteiramente casualizado, em parcelas subdivididas com três repetições. O efluente EDT utilizado no experimento foi proveniente da estação de tratamento de esgoto doméstico do tipo decanto digestor do Projeto de Assentamento Milagre Apodi/RN. O estudo foi realizado em viveiro de produção de mudas da UFERSA, sendo realizadas avaliações de crescimento e vigor aos 30, 60, 90 e 120 dias após o transplantio. A produção de mudas fertigadas com efluente doméstico promoveu diferenças em todas as variáveis de crescimento com melhoria na produção de fitomassa. As mudas fertigadas com diluição de 50% do EDT e 50% água de abastecimento proporcionaram melhores resultados quando produzidas com o substrato composto por solo e fibra de coco. As mudas apresentaram-se como de boa qualidade e as cultivadas com 50% e 75% de água residuária mostraram-se com maior qualidade aos 120 dias de cultivo.

Abstract The objective of this study was to verify the flight radius and the influence of the climatic season and period of the day on the external activity of Melipona rufiventris bees. The forager bees were released at different distances to evaluate the flight radius. The following were considered for external activities in the four different seasons of the year (Winter, Autumn, Spring, Summer): the entry with no apparent load was considered as nectar/water, entry with defined and opaque mass in the corbicula was considered as pollen, the entry with undefined and shiny mass in the corbicula was considered as resin/clay or bee exit no load and removal of debris, mass trapped by the jaws. Assessments were performed between 6 am and 6 pm each month. M. rufiventris can reach distances of 2 500 meters, however the return decreases as the distance increases. The species performs all activities in and out of the colony during all seasons of the year and periods between 6 am and 6 pm but reduce nectar/water collection and exit from the box without apparent load and with debris between 6:00 am and 10 am in winter. It is concluded that distances greater than 1 500 meters hinder the external activity of bees which is influenced by air temperature, air humidity, time of day, season of the year and food availability.

Resumo Objetivou-se com esse estudo verificar o raio de voo e a influência da estação climática e período do dia na atividade externa das abelhas Melipona rufiventris. As abelhas forrageiras foram soltas em diferentes distâncias para avaliar o raio de voo. Para as atividades externas nas quatro diferentes estações do ano (Inverno, Outono, Primavera, Verão) foram consideradas: a entrada sem carga aparente como néctar/água, a entrada com massa definida e opaca na corbícula caracterizada como pólen, a entrada com massa indefinida e brilhante na corbícula caracterizada como resina/argila ou ainda a saída de abelha sem carga e remoção de detritos, massa presa pelas mandíbulas. As avaliações foram realizadas entre 6h e 18h de cada mês. M. rufiventris pode atingir distâncias de 2500 metros, porém o retorno diminui à medida que a distância aumenta. A espécie realiza todas as atividades dentro e fora da colônia durante todas as estações do ano e períodos entre 6h e 18h, mas reduz a coleta de néctar/água e saída caixa sem carga aparente e com detritos entre 6h e 10h no inverno. Conclui-se que distâncias superiores a 1500 metros reduzem a atividade externa das abelhas que é influenciada pela temperatura e umidade do ar, hora do dia, estação do ano e disponibilidade de alimentos.

Abstract Low density sugarcane plantation (LDSP) has been implemented by some sugarcane producers in Brazil, aiming to save seeds and operational costs. The study was carried out in the municipality of Areia, Paraíba, Brazil. Five planting densities were used, varying from 5 to 25 m-2 of buds arranged in randomized blocks, with four replications. Data were measured annually over three cultivation cycles (2017 to 2020), during which the field was fertilized with NPK and the harvests were carried out manually without prior burning. The lower planting density presents higher productivity only in the cane plant (101.03 t ha-1) due to the higher plant height (2.37 m) and the higher number of stalks (11 stalks m-2), suggesting that these variables are due to the greater availability of light, water and photosynthate. However, there is a drastic reduction in sugarcane yield for this lower population in the 2nd ratoon by up to 65.62%, which is correlated with number of stalks per meter. We demonstrate the agronomic viability of LDSP in the population of 10 buds m-2 in relation to conventional planting of sugarcane until the 2nd ratoon. Data are important for future studies to present additional considerations for other production factors, such as the effects of mechanized harvesting and the management of nutrients and water, assessing the sustainability of this large-scale planting system.

Resumo O método de plantio de cana-de-açúcar de baixa densidade (LDSP) vem sendo implementado por alguns produtores de cana-de-açúcar no Brasil, visando economia de sementes e custos operacionais. O estudo foi realizado no município de Areia, Paraíba, Brasil. Utilizou-se cinco densidades de plantio, variando de 5 a 25 m-2 de gemas dispostas em blocos casualizados, com quatro repetições. Os dados foram medidos anualmente ao longo de três ciclos de cultivo (2017 a 2020). Menor densidade de plantio mostrou maior produtividade (101,03 t ha-1) devido à maior altura de planta (2,37 m) e ao maior número de hastes (NS) (11 hastes m-2), sugerindo que essas variáveis são devido à maior disponibilidade de luz, água e fotoassimilados. No entanto, houve redução drástica na produtividade da cana-de-açúcar para esta população menor na 2ª soca em 65,62%, estando correlacionado com o número de colmos por metro. Demonstramos a viabilidade agronômica do LDSP em uma população de 10 gemas m-2 em relação ao plantio convencional de cana-de-açúcar até a 2ª soca. Os dados são importantes para que estudos futuros apresentem considerações adicionais sobre outros fatores de produção, como os efeitos da colheita mecanizada e do manejo de nutrientes e água, avaliando a sustentabilidade desse sistema de plantio em larga escala.

Abstract Jackfruit is an exotic fruit present in the Northeast region of Brazil, presenting a great economic potential for the region because of the internal and external commercialization of its fruit. Its seed is rich in carbohydrates, protein, minerals and vitamins, and is an alternative source of starch and for food. Germination is an easily accessible, inexpensive technology for the production of sprouts, improving the bioavailability of nutrients in the seed for consumption, such as proteins and bioactive compounds. The objective of this work was to evaluate the chemical, physical and physicochemical parameters of fresh and germinated jackfruit seeds. The germination of jackfruit seeds increased the contents of water, proteins, ascorbic acid, phenolic compounds and total sugars, in addition to reducing the content of starch, lipids, tannins and the parameters of color, luminosity, red (+ a*) and yellow (+b*) intensities. In relation to the physical properties of the seed, there was an increase in the real specific mass and in the unit mass. For apparent specific mass there was a reduction. Seed germination provided an increase in the levels of magnesium, iron and sodium, consequently reducing potassium, phosphorus, calcium, zinc, copper and manganese.

Resumo A jaca é um fruto exótico presente na região Nordeste do Brasil, apresentando um grande potencial econômico para região em face da comercialização interna e externa do seu fruto. Sua semente é rica em carboidratos, proteína, minerais e vitaminas, constituindo fonte alternativa de amido para alimentação. A germinação é uma tecnologia barata de fácil acesso para produção de brotos, melhorando a biodisponibilidade de nutrientes na semente para o consumo, a exemplo das proteínas e compostos bioativos. O objetivo do trabalho foi avaliar os parâmetros químicos, físicos e físico-químicos das sementes de jaca in natura e germinadas. A germinação das sementes de jaca proporcionou o aumento nos teores de água, proteínas, ácido ascórbico, compostos fenólicos e açúcares totais, além de reduzir os conteúdo de amido, lipídios, taninos e os parâmetros de cor, a luminosidade, intensidade de vermelho (+a*) e de amarelo (+b*). Em relação às propriedades físicas da semente, ocorreu acréscimo na massa específica real e na massa unitária. Para massa específica aparente houve redução. A germinação das sementes proporcionou aumento nos teores de magnésio, ferro e sódio, consequentemente ocorrendo redução de potássio, fósforo, cálcio, zinco, cobre e manganês.

RESUMO A irregularidade das chuvas na região semiárida do Brasil e do mundo exerce ação limitante na produção agrícola, em geral, inclusive das espécies frutíferas. Nessas condições, a irrigação é o insumo que mantém o sistema produtivo com viabilidade econômica e social. O estudo foi desenvolvido no período de 2012 a 2014, no município de Remígio, Estado da Paraíba, Brasil, com o objetivo de avaliar a produção de gravioleira ‘Morada’ cultivada em condições de sequeiro e irrigada, no solo sem e com cobertura morta em duas safras. Os tratamentos foram distribuídos em blocos casualizados com três repetições, em arranjo fatorial 2 × 3 × 2, relativos às plantas não irrigadas (lâmina de 0 mm) e irrigadas semanalmente (lâmina de 17,2 mm) no solo sem cobertura, com cobertura a partir de restos de culturas e com bagaço de sisal nas safras agrícolas de 2012/2013 e 2013/2014. No solo sem cobertura a irrigação elevou a produtividade de 5,3 para 34,7 t ha-1 na primeira safra e de 9,4 para 57,7 t ha-1 na segunda safra. O uso conjunto da irrigação e a cobertura com resíduo de sisal proporcionou as maiores produtividades. O uso do resíduo de sisal como cobertura morta aumentou o número e a massa média de frutos, a produção por planta e a produtividade da gravioleira ‘Morada’ em relação ao solo sem cobertura e coberto por resíduos culturais.

ABSTRACT Irregular rainfall in the semiarid region of Brazil and in the world exerts a limiting action on agricultural production, in general, including fruit species. Under these conditions, irrigation is the input that keeps the production system both economically and socially viable. This study was carried out from 2012 to 2014, in the municipality of Remígio, Paraíba State, Brazil, aiming at evaluating the production of ‘Morada’ soursop cultivated under rainfed and irrigated conditions, in soil with and without mulch, in two harvests. The treatments were distributed in randomized block design with three replicates in a 2 × 3 × 2 factorial scheme, corresponding to non-irrigated plants (0 mm water depth) and plants irrigated weekly (17.2 mm water depth) in soil without mulching, with cover crop residues, and with a sisal bagasse mulch in the 2012/2013 and 2013/2014 crop years. In the soil without mulching, irrigation increased yield from 5.3 to 34.7 t ha-1 in the first harvest and from 9.4 to 57.7 t ha-1 in the second harvest. The association between irrigation and mulch with sisal residue promoted the highest yield. The use of mulch of sisal residue increased the number and mean mass of fruits, production per plant and yield of ‘Morada’ soursop compared to the soil without mulch and covered by crop residues.

ABSTRACT The simulation of terrestrial ecosystem processes, using numerical biosphere-atmosphere models that can be coupled to the Atmospheric Models, assist in a better diagnosis and forecast of climate and weather. To be able to represent a particular region, biome or ecosystem, the model parameters need to be fitted for local conditions. This work aims to assess the Luus-Jaakola (LJ) method in the optimization of the parameters in a two-stream radiative transfer model applied to a vegetation canopy. Solar radiation components (incident, S ↓, and reflected, S ↑) were measured above a sugarcane crop in a Tropical region from February 17 to 24, 2006. Among the combinations of internal and external iterations evaluated for Luss-Jaakola method, 60/30 (external/internal) iterations presented more precise albedo ( α = S ↑ / S ↓ ) simulated ( r 2 = 0 . 7386 ) and, for the accuracy of the simulated α, even though the 60/40 combination had the smallest percentual error (6.40%), the 60/30 combination was 0.03% higher. The precision and accuracy of S ↑ was greater with the parameters obtained by the inverse problem with the combination of 60/30 (external/internal) iterations respectively. In general, the behavior of simulated S ↑ at the top of the canopy was underestimated compared to the observed S ↑, especially in the early morning. For the simulated α at the top of the canopy, the model’s overestimation was observed at the lowest values of albedo. When the largest albedos are observed, only at the beginning of the day the model underestimated the values. As shown by the tests result, the parameters optimized by Luus-Jaakola method have an adequate representation of the observed data.

Abstract The present study consisted in optimizing the extractive method of polyphenols and total tannins of leaves of Syzygium cumini (L) Skeels assisted by microwaves to potentiate the antimicrobial activity of the dried extract of S. cumini against sensitive and resistant strains. A Box-Behnken design that consisted of 27 experimental runs coupled with a desirability function for multiple response optimization was employed to optimize the total polyphenols content and total tannins content. Antimicrobial sensitivity tests were evaluated by obtaining the minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration in 96-well petri dishes. The optimal extraction conditions were found to be 8 min of extraction, under 300 w of microwave power, using a 1:34 g/mL solid/solvent ratio and 38% of ethanol concentration as extraction solvent. The parameter with the greatest influence in the extraction was primarily the time, followed by the potency and proportion solid/solvent. This yielded a total polyphenol content of 87.37 ± 1.85 mg TAE g-1ext and a total tannin content of 79.68 ± 1.64 mg TAE g-1 ext. All tested microorganisms were sensitive to the extract, evidencing the effectiveness of the extraction method optimization.

ABSTRACT Jatropha curcas L. (Euphorbiaceae) is a plant species widely present in tropical and subtropical regions that have been studied mainly for its potential for biofuel production; it is also a plant known for its toxic substances. To explore its use as an insecticide, we studied the effect of the seed extract from green fruit and the seed oils from the mature and dried fruit of J. curcas on the green aphid Myzus persicae (Hemiptera: Aphididae). Seven concentrations of extracts and oils of different maturation stages of fruits were used and sprayed with the Potter Spray Tower on cabbage leaves containing 10 individuals of M. persicae. Ten repetitions for each concentration were carried out at each stage of maturation (green, ripe, and dry), and the evaluation of the mortality of individuals occurred for a period of 72 hours. The maturation stage of J. curcas, as well as the concentration and time after the application, influenced the mortality of M. persicae. The best results were obtained 48h after applying the dried fruit oil at 2.5% and 72 hours after the application of dry and ripe fruit oil at 2.5%.

RESUMEN La Jatropha curcas L. (Euphorbiaceae) es una especie vegetal, ampliamente presente en regiones tropicales y subtropicales, que ha sido estudiada principalmente por su potencial para la producción de biocombustibles. Pero también es una planta conocida por sus sustancias tóxicas. Para verificar su uso como insecticida, estudiamos el efecto del extracto de semillas de frutos verdes y aceites de semillas de frutos maduros y secos de J. curcas sobre el pulgón verde Myzus persicae (Hemiptera: Aphididae). Siete soluciones, en diferentes concentraciones de los extractos y aceites, fueron utilizadas y pulverizadas con la Torre de Pulverización de Potter en hojas de repollo, con 10 individuos de M. persicae cada una. Se realizaron diez repeticiones para cada concentración en cada etapa de maduración (verde, maduro y seco) y la evaluación de la mortalidad de los individuos ocurrió en un período de 72 horas. El estadio de maduración de J. curcas, la concentración y el tiempo después de la aplicación influyeron la mortalidad de M. persicae. Los mejores resultados se obtuvieron 48 horas después de la aplicación del aceite de frutos secos a 2,5%, y 72 horas después del aceite de los frutos secos y maduros a 2,5%.

Abstract Syzygium cumini L. Skeels belongs to Myrtaceae family. This species has been recognized by its antidiabetic, anti-inflammatory, and antimicrobial activities. Despite ever-increasing scientific interest for this species there is no pharmacopeia method for characterization and standardization of S. cumini yet. So, toward this aim, the objective of this work was to develop an efficient analytical methodology able to determine polyphenols and tannins content from leaves hydroethanolic extract of S. cumini using Folin-Ciocalteu method by ultraviolet absorption spectrophotometry (UV-Vis). The analytical methodology was developed for the first time in the literature for leaves of this specie shown to be fast and low-cost with results expressed through tannic acid equivalent (TAE). Moreover, the methodology presented selectivity with maximum absorption at 706 nm wavelength, linearity with R2>0.99; limit of detection 0.275 µg TAE mL-1 and 0.102 µg TAE mL-1; limit of quantification 1.046 µg TAE mL-1 and 0.912 µg TAE mL-1 for total polyphenols and total tannins, respectively. Furthermore, the methodology was accurate with recover value greater than 98%, as well as exact, reproductive, and robust with coefficient of variation values less than 15% for both compounds. All the results are found within the fixed limits according to RDC 166/2017.

Abstract Much of the diversity of mushrooms is still unknown to science. In the search for new edible mushrooms, we began to look at the traditions of Amazonian forest peoples in order to learn about the potential for edible mushrooms among other species. In particular, the Yanomami people launched the encyclopedia of food (Sanoma) in 2016 to preserve their knowledge of their own food. They demonstrated how food production can be balanced with the forest and present dozens of mushrooms that are consumed by their communities. In view of the above information, the objective of this work is to present bromatological aspects of the Lentinus crinitus mushroom from an agroforestry homegarden in the Cerrado. The specimen was collected from an agroforestry homegarden with biome forest species and agronomic species, located in the Brazilian Cerrado, in the city of Goiânia, Goiás. L. crinitus mushrooms presented 61% humidity, containing 14% crude protein, 1.5% ether extract, 26% crude fiber, and 3.4% mineral matter. Its bromatological characteristics are similar to others of commercial fungi. There is great potential for the use of the Lentinus crinitus mushroom in agroforestry yards in the Brazilian Cerrado, reconciling environmental conservation with the diversification of the production of agroextractive products.

Mimosa bimucronata é uma espécie vegetal que pode ser utilizada em programas de reflorestamento, sendo necessários estudos iniciais para a produção de mudas. Devido à importância desta espécie e à escassez de informações sobre a germinação das sementes, esta trabalho teve por objetivo avaliar i) as características biométricas das sementes por análise multivariada, ii) o efeito de pré-tratamentos de sementes na germinação e iii) caracterizar morfologicamente os diferentes estágios de germinação e de plântula. Foram analisadas a biometria e massa das sementes e oito tratamentos pré-tratamentos: testemunha; ácido sulfúrico (98%) durante dois, cinco e 10 min; água quente a 70 e 80 °C durante 2 min; escarificação mecânica e água destilada durante 24 h. Foram avaliados os diferentes estágios de desenvolvimento da semente até à formação de plântula. As sementes foram expostas a dois ambientes - laboratório e viveiro telado. Foram avaliados a percentagem de germinação e emergência; índice de velocidade de germinação e emergência; e tempo médio de germinação e emergência. De acordo com a análise multivariada, as sementes foram classificadas em três grupos, sendo as classes II e III as mais indicadas para produção de mudas. A germinação com ácido sulfúrico e lixa foram as mais indicadas. A espécie apresenta 10 estágios de desenvolvimento.

Mimosa bimucronata is a plant species that can be used in reforestation programs, and initial studies for the production of seedlings are needed. Due to the importance of this plant species and the scarcity of information on seed germination, this research was carried out with the objective of evaluate i) the biometric characteristics of the seeds by multivariate analysis, ii) the effect of seed pretreatment on germination, and iii) to characterize morphologically the different stages of germination and seedling. The biometry and seed mass and eight pre-germination treatments were analyzed: control; sulfuric acid (98%) for two; five and 10 min; hot water at 70 to 80 °C for 2 min; mechanical scarification and distilled water for 24 h. The different stages of seed development were evaluated until seedling formation. The seeds were exposed to two environments - laboratory and greenhouse. The percentage of germination and emergence were evaluated; germination and emergence speed index; and average time of germination and emergence. According to the multivariate analysis, the seeds were classified into three groups, with classes II and III being the most suitable for seedling production. Germination with sulfuric acid and sandpaper were the most indicated. The species presents 10 stages of development.

RESUMO Objetivou-se, neste trabalho, avaliar mudanças nos atributos químicos de um Cambissolo háplico em duas áreas de manejo sustentável da caatinga e em uma área de cultivo agrícola convencional e se optou, para efeito de comparação, pela mata nativa (Caatinga). As áreas foram manejadas da seguinte forma: Nas áreas em que houve manejo sustentável foi feito raleamento na caatinga com sistema de savana (1) e na outra área raleamento em faixas (2); na área de cultivo agrícola convencional foram realizadas queimada, aração e gradagem (3) e a mata nativa (4). Os atributos químicos analisados foram: pH, condutividade elétrica, Ca2+, Mg2+, H + Al, P, Na+, K+ e matéria orgânica do solo (MOS) calculando-se CTC e saturação por bases. Os tratamentos foram avaliados por meio de análises multivariadas, em várias profundidades até 0,52 m. Verificou-se que na área de caatinga raleada em savana e em faixa, houve maior disponibilidade de bases trocáveis e MOS próximos aos 0,20 m de profundidade e na área com agricultura tradicional se restringe a 0,10 m. Na área raleada com sistema de savana o Na apresentou correlação negativa com os demais elementos indicando situação de antagonismo.

ABSTRACT The objective of this work was to evaluate changes in the chemical attributes of an Inceptisol in two areas of sustainable management of the Caatinga Biome and a conventional tillage, compared with the native forest. The areas were managed in the following way: in areas under sustainable management, the Caatinga was thinned in a savanna system (1), and in the other area, the Caatinga was thinned in strips (2); the area under conventional tillage was burned, plowed and harrowed (3), and the native forest (4). The following chemical attributes were analyzed: pH, electrical conductivity, Ca2+, Mg2+, H + Al, P, Na+, K+ and soil organic matter (SOM), calculating CEC and base saturation. The treatments were evaluated using multivariate analysis, at different depths up to 0.52 m. In the Caatinga areas thinned in a savanna system and in strips, the greater availability of exchangeable bases and SOM occurs close to the depth of 0.20 m and, in the area with traditional agriculture, it is limited to 0.10 m. In the area thinned in a savanna system, sodium showed a negative correlation with the other elements.

Four new epimeric spirostanol and furostanol-type steroidal saponins, (25R,S)-5α-spirostan-2α,3β-diol 3-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl-(1→4)-β-D-galactopyranoside, (25R,S)-5α-spirostan-2α,3β-diol 3-O-β-D-glucopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1→4)-β-D-galactopyranoside, 26-O-β-D-glucopyranosyl-(25R,S)-5α-furost-20-ene,2α,3β-diol 3-O-β-D-galactopyranoside and 26-O-β-D-glucopyranosyl-(25R,S)-5α-furost-20-ene,2α,3β-diol 3-O-β-D-galactopyranosyl-(1→4)-β-D-galactopyranoside, in addition to the known (25R,S)-5α-spirostan-2α,3β-diol 3-O-β-D-galactopyranoside, were isolated from Cestrum laevigatum. Compounds were submitted to cytotoxic activity assays using colorectal adenocarcinoma (HCT-116), human promyelocytic leukemia (HL-60), ovarian carcinoma (OVCAR-8), glioma (SF-295) human cancer cell lines, and the antimicrobial activity was evaluated against Candida parapsilosis, C. albicans, C. krusei, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis.