Pesquisa | SciELO

RESUMEN Objetivo. El objetivo fue evaluar el efecto de la variabilidad de las lluvias sobre la composición química, la degradabilidad in vitro y la producción de gas de cactus en la región Semiárida. Materiales y métodos. Este fue un diseño experimental completamente al azar en un arreglo factorial de 2 períodos de lluvia (lluvia alta y baja) x 5 especies de cactus, con 5 repeticiones. Los cactus evaluados fueron Pilosocereus gounellei (Weber ex K. Schum). Bly ex Rowl, Cereus jamacaru DC., Opuntia ficus indica Mill, Nopalea cochenillifera Salm. Dyck y Opuntia stricta (Haw.) Haw, todas frescas. Resultados. Hubo una interacción entre la variación de la lluvia y las especies de cactus para materia seca, proteína cruda, fibra detergente neutra y fibra detergente ácido (p<0.05), producción de gas in vitro (p<0.05) y para la degradabilidad in vitro de materia seca (p<0.05). Entre los cactus, Pilosocereus gounellei presentó una menor proporción de materia orgánica y un aumento en el contenido de materia mineral en relación a los demás cactus (p<0.05). Conclusiones. La composición química, los coeficientes de producción de gas y la degradabilidad in vitro fueron influenciados por las especies de cactus y por la variación de las precipitaciones. Las especies de cactus aumentan el contenido de materia seca, proteína cruda y fibra de detergente neutro durante la escasez de precipitaciones. Las variedades de nopal (Opuntia y Nopalea) se destacaron entre los cactus ensayados en relación a la producción de gas y la degradabilidad in vitro.

ABSTRACT Objective. The objective was to evaluate the effect of rainfall variability on the chemical composition, in vitro degradability and gas production of cacti in Semiarid region. Materials and methods. A completely randomized experimental design in a factorial arrangement of 2 rainfall periods (high and low rainfall) x 5 cactus species, with 5 replications was adopted. Cacti evaluated were Pilosocereus gounellei (Weber ex K. Schum). Bly ex Rowl, Cereus jamacaru DC., Opuntia ficus indica Mill, Nopalea cochenillifera Salm. Dyckand Opuntia stricta (Haw.) Haw, all fresh. Results. There was an interaction between rainfall variation and cactus species for dry matter, crude protein, neutral detergent fiber and acid detergent fiber (p<0.05), in vitro gas production (p<0.05) and for in vitro dry matter degradability (p<0.05). Among cacti, Pilosocereus gounellei presented a lower proportion of organic matter and an increase in the content of mineral matter in relation to the other cacti (p<0.05). Conclusions. The chemical composition, the gas production coefficients and the in vitro degradability were influenced by the cacti species and by the rainfall variation. Cacti species increase the dry matter, crude protein and neutral detergent fiber contents during the low rainfall. Varieties of cactus pear (Opuntia and Nopalea) stood out among the cacti tested in relation to gas production and in vitro degradability.

https://doi.org/10.24188/recia.v14.n1.2022.895

Volume, biomass, carbon stock and efficiency of water use in irrigated eucalyptus
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Lopes, Adriano da Silva

; Folegatti, Marcos Vinicius

; Fanaya Júnior, Eder Duarte

; Oliveira, Gabriel Queiroz de

; Oliveira, Jean Carlos Lopes de

; Brito, Kelvin Melgar Rosalvo

; Rego, Norton Hayd

Ciência Florestal

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Abr 2022, Volume 32 Nº 2 Páginas 1047 - 1060

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Como o eucalipto é uma planta de crescimento rápido, oferece vantagens em comparação com outras espécies florestais, e o uso de sistemas de irrigação localizados pode aumentar o rendimento e reduzir o tempo de corte. O objetivo deste estudo foi avaliar o crescimento de dois híbridos de eucalipto, em condição de cultura: terra irrigada e seca, durante o primeiro ano de desenvolvimento da cultura. O experimento foi realizado em abril de 2011 na área experimental de irrigação da Universidade Estadual de Mato Grosso do Sul, no município de Aquidauana, MS, Brasil. O delineamento experimental foi em blocos casualizados, com quatro blocos e duas repetições em cada bloco. As parcelas foram compostas pelos tratamentos de irrigação (gotejamento, microaspersão e sem irrigação), e as subparcelas foram compostas por dois híbridos de eucalipto (Grancam e Urograndis). As avaliações ocorreram aos 60, 120, 180, 240, 300 e 360 dias após o transplante (DAT), medindo a altura das plantas e o diâmetro do tronco; e, assim, foram estimados o volume, a biomassa, o estoque de carbono e a eficiência no uso da água em todos os períodos avaliados. Os dados foram submetidos à análise de variância e comparados pelo teste de Tukey ao nível de 5% de probabilidade. Conclui-se que, a 360 DAT, as irrigações por gotejamento e microaspersão levaram a ganhos de biomassa e estoque de carbono de cerca de 190% em comparação com o eucalipto sem irrigação.

Abstract Since eucalyptus is a fast-growing plant, it offers advantages in comparison to other forest species, and the use of localized irrigation systems can increase yield and reduce time to cutting. The aim of this study was to evaluate two eucalyptus hybrids, growth in crop condition: irrigated and dry land, during the first year of development of the crop. The experiment was set up in April 2011 in the irrigation experimental area of the State University of Mato Grosso do Sul, in the municipality of Aquidauana, Mato Grosso do Sul, Brazil. A randomized block experimental design was use in split-plots, with four blocks and two replications within each block. Plots were composed of the irrigation treatments (drip, micro sprinkler and no irrigation), and the subplots were composed of two eucalyptus hybrids (Grancam and Urograndis). Evaluations occurred at 60, 120, 180, 240, 300 and 360 days after transplant (DAT), measuring the height of plants and trunk diameter; and, thus, the volume, biomass, carbon stock and efficiency in water use in all the periods evaluated was estimated. Data were subject to analysis of variance and compared by the Tukey test at the level of 5% probability. It is concluded that at 360 DAT, the drip and micro sprinkler irrigations led to gains in biomass and carbon stock of around 190% as compared to eucalyptus without irrigation.

https://doi.org/10.5902/1980509847949

Probiotic milk drink as adjuvant therapy for the treatment of periodontitis: a randomized clinical trial with 180 days follow-up
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BILOURO, Fabio Cascardo

; ROCHA, Ramon Silva

; GUIMARÃES, Jonas Toledo

; PIMENTEL, Tatiana Colombo

; MAGNANI, Marciane

; ESMERINO, Erick Almeida

; FREITAS, Monica Queiroz de

; SILVA, Marcia Cristina

; CRUZ, Adriano Gomes da

; CANABARRO, Antonio

Food Science and Technology

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2022, Volume 42 elocation e17922

Resumo: En | Texto: En | PDF: En

Abstract The effect of probiotic milk drinks associated with non-surgical periodontal therapy (NSPT) in treating periodontitis was evaluated. A double-blind placebo-controlled randomized clinical trial with patients with periodontitis was conducted. Two groups were used: TG (n=15, test group, probiotic milk drink, Lacticaseibacillus casei 01, 8-9 log CFU/mL) and CG (n=15, control group, conventional milk drink). The milk drinks (100 mL) were consumed once a day at breakfast for 15 days. Both groups also received NSPT, supra and subgingival scaling and root planning per quadrant, and oral hygiene instruction. All patients were clinically evaluated after 30, 90, and 180 days of the last NSPT session. Reductions in clinical attachment loss and probing pocket depth were observed at 30 days in both groups, maintained throughout the follow-up. Decreases in visible plaque index (VPI) and bleeding on probing were observed after probiotic milk drink consumption for 30 days, suggesting an effect provided by probiotics in the control of biofilm and inflammation. However, the impact on VPI was not persistent for more than 30 days, demonstrating the need for regular consumption of the probiotic milk drink. In conclusion, probiotic milk drinks may be used as adjuvant therapy to treat periodontitis.

https://doi.org/10.1590/fst.17922

Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants
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Pais, Ana Karolina Leite

; Santos, Leandro Victor Silva dos

; Albuquerque, Greecy Mirian Rodrigues

; Farias, Antonio Roberto Gomes de

; Silva Junior, Wilson José

; Balbino, Valdir de Queiroz

; Silva, Adriano Márcio Freire

; Gama, Marco Aurelio Siqueira da

; Souza, Elineide Barbosa de

Genetics and Molecular Biology

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2022, Volume 45 Nº 4 elocation e20220038

Resumo: En | Texto: En | PDF: En

Abstract Banana tree bacterial wilt is caused by the Ralstonia solanacearum Moko ecotype. These strains vary in their symptom progression in banana, and are classified as typical Moko variants (phylotype IIA and IIB strains from across Central and South America), Bugtok variant (Philippines), and Sergipe facies (the states of Sergipe and Alagoas, Brazil). This study used comparative genomic and phylogenomic approaches to identify a correlation between the symptom progression of the Moko ecotypes based on the analysis of 23 available genomes. Average nucleotide identity and in silico DNA-DNA hybridization revealed a high correlation (>96% and >78%, respectively) between the genomes of Moko variants. Pan-genome analysis identified 21.3% of inheritable regions between representatives of the typical Moko and Sergipe facies variants, which could be traced to an abundance of exclusive homolog clusters. Moko ecotype genomes shared 1,951 orthologous genes, but representatives with typical symptoms did not display unique orthologues. Moreover, Bugtok disease and Sergipe facies genomes did not share any unique genes, suggesting convergent evolution to a shared symptom progression. Overall, genomic and phylogenomic analyses were insufficient to differentiate the Moko variants based on symptom progression.

https://doi.org/10.1590/1678-4685-gmb-2022-0038

ÓBITOS POR COVID-19 EM TRABALHADORES DA ENFERMAGEM BRASILEIRA: ESTUDO TRANSVERSAL
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Barreto, Márcio Adriano Fernandes

; Pessoa, Graça Rocha

; Queiroz Neto, João Bezerra de

; Chaves, Edna Maria Camelo

; Silva, Lucilane Maria Sales

; Moreira, Thereza Maria Magalhães

Cogitare Enfermagem

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2022, Volume 27 elocation e83824

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: analisar óbitos por Covid-19 em profissionais da enfermagem brasileira, confirmados com Covid-19, e em quarentena, internados ou falecidos, no Observatório do Conselho Nacional de Enfermagem. Método: estudo transversal, entre março de 2020 e agosto de 2021, no Brasil, via observatório do Conselho Federal de Enfermagem. Os dados foram analisados utilizando estatística descritiva e inferencial. Resultados: a prevalência de óbito por Covid-19 em profissionais da enfermagem brasileira foi de 2,5% (825/32.560), nos auxiliares de enfermagem 4,9% (109/2.224), estes com média de idade mais elevada (p< 0,0001). Apesar de 84,5% (27.508/32.560) da amostra ser feminina, a porcentagem de óbito foi maior no sexo masculino 5,2% (263/5.052) (p<0,0001). A regressão logística demonstrou que o sexo e a categoria profissional podem predizer o óbito por Covid-19 entre a enfermagem brasileira. Conclusão: o estudo contribui para evidenciar os riscos da categoria no enfrentamento da Covid-19 e repensar medidas para reduzir danos da pandemia nessa população.

ABSTRACT Objective: to analyze deaths due to COVID-19 in Brazilian Nursing professionals, with confirmed diagnoses of the disease and in quarantine, hospitalized or deceased, at the Federal Nursing Council Observatory. Method: a cross-sectional study conducted in Brazil between March 2020 and August 2021, via the Federal Nursing Council Observatory. The data were analyzed using descriptive and inferential statistics. Results: the prevalence of death due to COVID-19 in Brazilian Nursing professionals was 2.5% (825/32,560) and 4.9% (109/2,224) in nursing assistants, these latter with a higher mean age (p<0.0001). Although 84.5% (27,508/32,560) of the sample was female, the percentage of deaths was higher in the male gender, with 5.2% (263/5,052) (p<0.0001). The logistic regression analysis showed that gender and professional category can predict deaths due to COVID-19 among Brazilian Nursing workers. Conclusion: the study contributes to evidencing the risks faced by the professional category in coping with COVID-19, as well as to rethinking measures to reduce the harms imposed by the pandemic on this population segment.

RESUMEN Objetivo: analizar los fallecimientos por COVID-19 entre profesionales de Enfermería brasileños, con diagnóstico confirmado de la enfermedad y en cuarentena, internados o fallecidos, en el Observatorio del Consejo Federal de Enfermería. Método: estudio transversal realizado en Brasil entre marzo de 2020 y agosto de 2021 a través del Observatorio del Consejo Federal de Enfermería. Los datos se analizaron por medio de estadística descriptiva e inferencial. Resultados: la prevalencia de fallecimientos por COVID-19 entre profesionales de Enfermería brasileños fue del 2,5% (825/32.560) y en auxiliares de enfermería fue del 4,9% (109/2.224), estos últimos con mayor media de edad (p<0,0001). A pesar de que el 84,5% (27.508/32.560) de la muestra estuvo compuesta por mujeres, el porcentaje de fallecimientos fue mayor en el sexo masculino: 5,2% (263/5.052) (p<0,0001). El análisis de regresión logística demostró que el sexo y la categoría profesional pueden predecir el desenlace de fallecimiento por COVID-19 entre profesionales de Enfermería en Brasil. Conclusión: el estudio contribuye a poner de manifiesto los riesgos a los que se expone la categoría profesional en el afrontamiento del COVID-19, al igual que a repensar medidas para reducir los perjuicios ocasionados por la pandemia en este segmento de la población.

https://doi.org/10.5380/ce.v27i0.83824

Genomic sequencing of different sequevars of Ralstonia solanacearum belonging to the Moko ecotype
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Pais, Ana Karolina Leite

; Silva, Jessica Rodrigues da

; Santos, Leandro Victor Silva dos

; Albuquerque, Greecy Mirian Rodrigues

; Farias, Antonio Roberto Gomes de

; Silva Junior, Wilson José

; Balbino, Valdir de Queiroz

; Silva, Adriano Márcio Freire

; Gama, Marco Aurélio Siqueira da

; Souza, Elineide Barbosa de

Genetics and Molecular Biology

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2021, Volume 44 Nº 1 elocation e20200172

Resumo: En | Texto: En | PDF: En

Abstract Banana vascular wilt or Moko is a disease caused by Ralstonia solanacearum. This study aimed to sequence, assemble, annotate, and compare the genomes of R. solanacearum Moko ecotypes of different sequevar strains from Brazil. Average nucleotide identity analyses demonstrated a high correlation (> 96%) between the genome sequences of strains CCRMRs277 (sequevar IIA-24), CCRMRs287 (IIB-4), CCRMRs304 (IIA-24), and CCRMRsB7 (IIB-25), which were grouped into phylotypes IIA and IIB. The number of coding sequences present in chromosomes and megaplasmids varied from 3,070 to 3,521 and 1,669 to 1,750, respectively. Pangenome analysis identified 3,378 clusters in the chromosomes, of which 2,604 were shared by all four analyzed genomes and 2,580 were single copies. In megaplasmids, 1,834 clusters were identified, of which 1,005 were shared by all four genomes and 992 were identified as single copies. Strains CCRMRsB7 and CCRMRs287 differed from the others by having unique clusters in both their chromosomes and megaplasmids, and CCRMRsB7 possessed the largest genome among all Moko ecotype strains sequenced to date. Therefore, the genomic information obtained in this study provides a theoretical basis for the identification, characterization, and phylogenetic analysis of R. solanacearum Moko ecotypes.

https://doi.org/10.1590/1678-4685-gmb-2020-0172 901 downloads

A summary of the proceedings of a meeting on the treatment of latent tuberculosis infection in target populations in Brazil
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Rolla, Valeria

; Trajman, Anete

; Kawamura, Masae

; Cavalcante, Solange

; Soares, Elizabeth

; Perini, Filipe de Barros

; Carvalho, Anna Cristina Calçada

; Croda, Julio

; Silva, Jose Roberto Lapa e

; Schmaltz, Carolina Arana Stanis

; Sant’Anna, Flavia Marinho

; Bastos, Mayara

; Gomes, Adriano

; Durovni, Betina

; Saraceni, Valeria

; Silva, Elisangela

; Mello, Fernanda Carvalho de Queiroz

; Santos, Marcelo Cordeiro dos

; Pinto, Marcia

; Santos, Marisa

; Sanches, Fernando Augusto Dias e

; Oliveira, Sandra Maria do Valle Leone de

Jornal Brasileiro de Pneumologia

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2020, Volume 46 Nº 4 elocation e20200023

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36416/1806-3756/e20200023 296 downloads

Genomic sequencing of two isolates of Ralstonia solanacearum causing Sergipe facies and comparative analysis with Bugtok disease isolates
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Silva, Jéssica Rodrigues da

; Pais, Ana Karolina Leite

; Albuquerque, Greecy Mirian Rodrigues

; Silva, Adriano Márcio Freire

; Silva Junior, Wilson José

; Balbino, Valdir de Queiroz

; Fonseca, Maria Esther Noronha

; Gama, Marco Aurélio Siqueira da

; Souza, Elineide Barbosa de

; Mariano, Rosa de Lima Ramos

Genetics and Molecular Biology

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2020, Volume 43 Nº 4 elocation e20200155

Resumo: En | Texto: En | PDF: En

Abstract Ralstonia solanacearum is the causal agent of Moko disease in bananas, which in the state of Sergipe in northeastern Brazil causes “Sergipe facies”. This disease induces atypical symptoms similar to those of Bugtok disease in the Philippines. This study was conducted to sequence, assemble, and annotate the genomes of the Sergipe facies-causing isolates SFC and IBSBF2570 (sequevar IIA-53) and compare their genomes with two representative isolates causing Bugtok disease. The genomes were sequenced and assembled, resulting in lengths of 5.58 Mb (SFC) and 5.46 Mb (IBSBF2570) in 185 and 174 contigs, respectively. The isolates of Sergipe facies and Bugtok disease showed similarities in their gene contents. We identified 5,668 information clusters, 3,752 of which were shared by all genomes (core genes). Moreover, 3,585 single-copy genes were identified. Isolates causing Bugtok disease exclusively shared 266 more information clusters than the isolates causing Sergipe facies. These results suggest that Sergipe facies and Bugtok disease isolates show high genomic similarity. However, the similarity is even greater between the Bugtok disease isolates. This may be because of their longer period of interaction compared to Sergipe facies isolates.

https://doi.org/10.1590/1678-4685-gmb-2020-0155 556 downloads

IRRIGAÇÃO NO DESENVOLVIMENTO INICIAL DE Eucalyptus urophylla x Eucalyptus grandis e Eucalyptus grandis x Eucalyptus camaldulensis
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Jung, Leandro Henrique

; Lopes, Adriano da Silva

; Oliveira, Gabriel Queiroz de

; Oliveira, Jean Carlos Lopes de

; Fanaya Júnior, Eder Duarte

; Brito, Kelvin Rosalvo Melgar

Ciência Florestal

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Jun 2017, Volume 27 Nº 2 Páginas 655 - 667

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste trabalho foi avaliar o desenvolvimento inicial de dois híbridos de eucalipto (Grancam e Urograndis), irrigados por gotejamento e microaspersão e sem irrigação dos 7 aos 17 meses após o plantio (MAP). O experimento foi instalado na área experimental de irrigação da Universidade Estadual de Mato Grosso do Sul, no município de Aquidauana - MS. O delineamento experimental utilizado foi blocos casualizados, em parcelas subdivididas, com quatro blocos e duas replicações dentro de cada bloco, sendo as parcelas compostas pelos tratamentos de irrigação (gotejamento, microaspersão e sequeiro) e as subparcelas pelos híbridos (Grancam e Urograndis). Foi avaliada a altura de plantas, o diâmetro de caule, a área basal de caule, a relação entre altura e diâmetro de caule, volume de caule por hectare e incremento médio mensal das variáveis analisadas. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste Tukey em nível de 5% de probabilidade de erro. Em todos os meses os sistemas de irrigação por gotejamento e microaspersão propiciaram maior altura de plantas, diâmetro do caule, área basal do caule e volume de caule por hectare. O híbrido Grancam apresenta parâmetros dendrométricos superiores ao Urograndis.

ABSTRACT The aim of this study was to evaluate the initial development of two hybrids of eucalypt (Grancam and Urograndis), microsprinkler and drip irrigated and non-irrigated from 7 to 17 months after planting (MAP). The experiment was installed in the area of irrigation and experimental demonstration of ‘Universidade Estadual de Mato Grosso do Sul’ in Aquidauana, Mato Grosso do Sul state, Brazil. The experimental design was a randomized block, split plot design with four blocks and two replications in each block, whereas the plots were composed by irrigation treatments (microsprinkler, drip and non-irrigated) and the subplots hybrids (Grancam and Urograndis). We evaluated the plant height, stem diameter, stem basal area, the relationship between height and stem diameter, stem volume per hectare and increased average monthly variables. The data were subjected to analysis of variance and compared by Tukey test at 5% probability. The irrigation systems microsprinkler and drip provide greater plant height, stem diameter, basal area of the stem and stem volume per hectare. The hybrid Grancam presents dendrometric parameters above those of Urograndis.

https://doi.org/10.5902/1980509827750 922 downloads

10.

MORPHO-ANATOMICAL CHANGES OF PEQUI LEAVES (Caryocar brasiliense Cambess.) EXPOSED TO SIMULATED DRIFT OF GLYPHOSATE
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Silva, Lailla Queiroz

; Jakelaitis, Adriano

; Vasconcelos Filho, Sebastião Carvalho

; Costa, Alan Carlos

; Araújo, Alana Cristina Ferreira

Revista Árvore

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Ago 2016, Volume 40 Nº 4 Páginas 669 - 677

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo desta pesquisa foi avaliar a fitointoxicação e a anatomia foliar de plantas de pequi (Caryocar brasiliense Cambess.) submetidas à deriva simulada do glifosato. O delineamento utilizado foi em blocos casualizados com nove repetições. A unidade experimental foi constituída por um vaso, com capacidade de 18 litros, contendo uma planta. Os tratamentos corresponderam à aplicação das doses de 0 (controle), 50, 100, 250, 500, 1000 e 1500 g e.a. ha-1 de glifosato. Avaliações visuais de fitointoxicação foram realizadas aos 7, 14 e 21 dias após a aplicação (DAA) dos tratamentos por meio de notas expressas em escala percentual, em que zero e cem representaram ausência de sintomas e morte das plantas, respectivamente. Descrição dos sintomas, alterações na anatomia foliar e micromorfometria foram realizadas em folhas do ápice e do terço médio aos 23 DAA. Os sintomas visuais de intoxicação foram murcha, clorose seguida de necrose, enrolamento das folhas dos ápices e senescência foliar, sendo intensificadas com o aumento das doses. As alterações anatômicas nas folhas foram detectadas a partir da dose de 250 g ha-1. Os danos causados foram células plasmolisadas, ruptura da epiderme, células alteradas, hiperplasia, células colapsadas, tecidos necrosados e acúmulo de compostos fenólicos.

ABSTRACT The goal of this research was to examine phytotoxicity and leaf anatomy of pequi plants (Caryocar brasiliense Cambess.) exposed to simulated drift of glyphosate. The experimental design was randomized blocks with nine replications. Each experimental unit was composed by one 18-L pot with one plant. The treatments consisted of different doses of glyphosate sprayed: 0 (control), 50, 100, 250, 500, 1000 and 1500 g ae ha-1 of glyphosate. Phytotoxicity visual ratings were carried out at 7, 14 and 21 days after spraying (DAS) by scores expressed in a percentage scale, within which zero and one hundred represent no symptom and plant death, respectively. Description of symptoms, changes in leaf anatomy and micromorphometric analysis were performed on leaves taken from plant top and middle third at 23 DAS. Poisoning symptoms were wilting, chlorosis followed by necrosis, winding of top leaves and leaf senescence, being intensified with increasing doses. Leaf anatomical changes were detected from the dose of 250 g ha-1. The observed damages consisted of plasmolized cells, epidermal disruption, distorted cells, hyperplasia, cell collapsing, necrotic tissue and accumulation of phenolic compounds.

https://doi.org/10.1590/0100-67622016000400010 1537 downloads

11.

Distribution of the root system of peach palm under drip irrigation
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Lopes, Adriano da Silva

; Hernandez, Fernando Braz Tangerino

; Alves Júnior, José

; Oliveira, Gabriel Queiroz de

Acta Scientiarum. Agronomy

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Set 2014, Volume 36 Nº 3 Páginas 317 - 321

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

A incorporação de tecnologias vem trazendo aumento de produtividade e manejo mais racional da pupunheira, sendo a irrigação uma ferramenta importante para algumas regiões produtoras. Nesse sentido, estudos que levem ao manejo adequado da cultura são de extrema importância, como a estimativa da profundidade efetiva do sistema radicular da cultura, indispensável para o correto manejo de irrigação. O objetivo deste trabalho foi avaliar os efeitos de diferentes lâminas de irrigação, aplicadas por gotejamento, sobre a distribuição do sistema radicular da pupunheira. Este experimento foi conduzido em Ilha Solteira, Estado de São Paulo, com irrigação por gotejamento, com dois gotejadores autocompensantes de 0,0023 m³ h-1 por planta, constando de quatro tratamentos de irrigação que corresponderam a 0, 50, 100 e 150% da evaporação da água do tanque Classe 'A'. Após cinco anos, foi realizada a análise do sistema radicular das pupunheiras, à distância de 0,0; 0,5 e 1,0 metro do estipe, com amostragens em duas profundidades, de 0,0 a 0,3 m e 0,3 a 0,6 m, pelo método do trado (análise volumétrica), concluindo-se que a profundidade efetiva do sistema radicular da pupunheira, utilizada para o manejo da irrigação, deve ser de, no máximo, 0,3 metro.

The incorporation of technologies has resulted in increased productivity and the more rational management of peach palm, with irrigation being an important tool for certain regions. Thus, studies leading to proper crop management are extremely important, such as the estimate of the effective depth of the root system, which is indispensable for proper irrigation management. The objective of this study was to evaluate the effects of different irrigation depths, as applied by drip irrigation, on the distribution of the root system of peach palm. This experiment was conducted in Ilha Solteira, São Paulo State, Brazil, with drip irrigation, with the two systems (flow of 0.0023 m³ h-1) consisting of four irrigation treatments corresponding to 0, 50, 100 and 150% of Class 'A' pan evaporation. After five years, an analysis of the Bactris gasipaes root system was performed at a distance of 0.0, 0.5 and 1.0 meters from the trunk, collecting sampling at two depths (0.0 to 0.3 m and 0.3 to 0.6 m) via the auger method (volumetric analysis). We concluded that the effective depth of the root system used for irrigation management should be a maximum of 0.3 meters.

https://doi.org/10.4025/actasciagron.v36i3.16281 1174 downloads

12.

PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO
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Carvalho, Rosangela Nunes

; Oliveira, Antonio Nonato de

; Mesquita, Albenones José de

; Rezende, Cíntia Silva Minafra e

; Mesquita, Adriano Queiroz de

; Romero, Rolando Alfredo Mazzoni

Brazilian Journal of Microbiology

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2014, Volume 45 Nº 1 Páginas 07 - 10

Resumo: En | Texto: En | PDF: En

Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.

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13.

Inflammation in disseminated lesions: an analysis of CD4+, CD20+, CD68+, CD31+ and vW+ cells in non-ulcerated lesions of disseminated leishmaniasis
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Mendes, Dayana Santos

; Dantas, Marina Loyola

; Gomes, Juliana Menezes

; Santos, Washington Luis Conrado dos

; Silva, Adriano Queiroz

; Guimarães, Luiz Henrique

; Machado, Paulo R

; Carvalho, Edgar Marcelino de

; Arruda, Sérgio

Memórias do Instituto Oswaldo Cruz

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Fev 2013, Volume 108 Nº 1 Páginas 18 - 22

Resumo: En | Texto: En | PDF: En

Disseminated leishmaniasis (DL) differs from other clinical forms of the disease due to the presence of many non-ulcerated lesions (papules and nodules) in non-contiguous areas of the body. We describe the histopathology of DL non-ulcerated lesions and the presence of CD4-, CD20-, CD68-, CD31- and von Willebrand factor (vW)-positive cells in the inflamed area. We analysed eighteen biopsies from non-ulcerated lesions and quantified the inflamed areas and the expression of CD4, CD20, CD68, CD31 and vW using Image-Pro software (Media Cybernetics). Diffuse lymphoplasmacytic perivascular infiltrates were found in dermal skin. Inflammation was observed in 3-73% of the total biopsy area and showed a significant linear correlation with the number of vW+ vessels. The most common cells were CD68+ macrophages, CD20+ B-cells and CD4+ T-cells. A significant linear correlation between CD4+ and CD20+ cells and the size of the inflamed area was also found. Our findings show chronic inflammation in all DL non-ulcerated lesions predominantly formed by macrophages, plasmacytes and T and B-cells. As the inflamed area expanded, the number of granulomas and extent of the vascular framework increased. Thus, we demonstrate that vessels may have an important role in the clinical evolution of DL lesions.

2489 downloads Citado 1 vez em SciELO

14.

Manejos de irrigação e nitrogênio na produção de feijoeiro sob plantio direto
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Pacheco, Arthur

; Oliveira, Gabriel Queiroz de

; Lopes, Adriano da Silva

; Barbosa, Alessandro Santana

Pesquisa Agropecuária Tropical

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Set 2012, Volume 42 Nº 3 Páginas 323 - 330

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O feijoeiro vem sendo cultivado por produtores que dispõem dos mais variados níveis de tecnologia, os quais permitem que a cultura alcance maior rentabilidade no inverno. Este trabalho objetivou avaliar a produtividade do feijoeiro e seus componentes, em razão de diferentes manejos de irrigação e adubação nitrogenada, em plantio direto, em Aquidauana (MS). O experimento foi conduzido de junho a setembro de 2010, na Universidade Estadual de Mato Grosso Sul, em Argissolo Vermelho-Amarelo distrófico. Utilizou-se a cultivar Pérola, semeada em junho e irrigada por aspersão convencional. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subdivididas, compostas por três blocos e duas replicações dentro de cada bloco. As parcelas foram compostas por três manejos de irrigação (tanque Classe "A" (TCA), equação de Hargreaves-Samani e equação de Penman-Monteith) e as subparcelas por quatro doses de adubação nitrogenada (0 kg ha-1, 50 kg ha-1, 100 kg ha-1 e 150 kg ha-1). Os dados foram submetidos à análise de variância e comparados pelo teste Tukey (5%) e modelos de regressão. O feijoeiro obteve as maiores produtividades de grãos e número de vagens por planta utilizando-se o manejo de irrigação com TCA. Não houve influência das doses de nitrogênio para os parâmetros produtivos do feijoeiro.

Common bean has been cultivated by producers who have access to a wide range of technologies that allow a higher profitability in the winter. This study aimed at evaluating bean yield and its components, under no-tillage system, according to different irrigation and nitrogen fertilization managements, in Aquidauana, Mato Grosso do Sul State, Brazil. The experiment was carried out from June to September (2010), in the Universidade Estadual de Mato Grosso do Sul, in an Alfisol. The "Pérola" cultivar was sown in June and irrigated by conventional sprinkling. The experimental design was randomized blocks, with split-plots consisting of three blocks and two replications in each block. The plots consisted of three irrigation systems (Class "A" pan and Hargreaves-Samani and Penman-Monteith equations) and the subplots of four nitrogen fertilizing doses (0 kg ha-1, 50 kg ha-1, 100 kg ha-1 and 150 kg ha-1). Data were evaluated with the aid of variance analysis and compared by the Tukey test (5%) and regression models. Bean plants reached the highest grain yield and number of pods per plant when the Class "A" pan irrigation system was used. Nitrogen doses did not affect bean yield.

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15.

Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins
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Mesquita, Adriano Queiroz de

; Rezende, Cintia Silva Minafra e

; Mesquita, Albenones José de

; Jardim, Eurione Antonio Garcia da Veiga

; Kipnis, Ana Paula Junqueira

Brazilian Journal of Microbiology

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Jun 2012, Volume 43 Nº 2 Páginas 692 - 697

Resumo: En | Texto: En | PDF: En

The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent of those samples with SCC above 200,000 (n=13) were screened for real-time PCR identification of microorganisms and blood samples were genotyped for TLR4 SNPs. There was a higher prevalence of Gram-positive bacteria in the analyzed samples (88.9%) and animals that had the combined genotypes AACCCC, GGTCGG and GACCGC presented the lowest somatic cell scores, and consequently those genotypes have the potential to be applied as molecular markers for assisted animal selection to improve milk quality.

3226 downloads Citado 1 vez em SciELO

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