Resumen Este estudio tuvo como objetivo evaluar el efecto de la inoculación con L. buchneri liofilizado y/o activado sobre las poblaciones de bacterias de ácido láctico en ensilajes de maíz. Los tratamientos experimentales consistieron en ensilaje de maíz sin aditivo y ensilaje con los inoculantes L. buchneri 1 × 10 5 ufc/g, aplicados según las recomendaciones del fabricante (1 g/tonelada de forraje) en las formas de inoculante liofilizado e inoculante preactivado. Se identificaron aislados purificados de ensilajes de maíz con y sin inocu lación, y el 93% de los aislados correspondieron a bacterias de ácido láctico de la especie L. plantarum. Entre los aislados no se detectaron bacterias de la especie L. buchneri. La aplicación de L. buchneri liofilizado o activado en la masa ensilada promueve un aumento considerable de la población de bacterias de ácido lácticas en los ensilajes de maíz durante todo el período de fermentación.

Abstract This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 10 5 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.

Abstract The growing demand for multiparameter sensors includes compact devices accompanied by simple calibration processes to distinguish the outputs from each other. This paper evaluates a scheme to determine multiple parameters of a medium using localized surface plasmon resonances (SPR) excited on a Dshaped photonic crystal fiber (PCF) partially covered by two gold layers of different thicknesses. We demonstrate that the proposed sensing platform, once customized to characterize the possible dispersive profiles of the refractive index of the analyte, also allows interrogating the temperature of a sample from a linear relationship. Since the plasmonic resonances are excited at separated and low crosstalk spectral channels, different sensing responses can be obtained simultaneously in the same location of the D-shaped PCF. These features turn out the SPR sensor a suitable tool for simultaneous monitoring of optical dispersion and temperature. other (SPR PCF (PCF thicknesses platform analyte relationship channels D shaped

Abstract Objective The aim of the present study is to determine whether previous shoulder and knee injuries were associated with isokinetic fatigue index and agonist/antagonist ratio of shoulder internal/external rotators and knee flexors/extensors in male volleyball athletes. Methods The current study is a cross-sectional investigation of 49 male elite volleyball players competing at a high level in Brazil. Isokinetic fatigue index and agonist/antagonist profiles were assessed during the preseason. Additionally, in order to record previous injuries, the athletes answered a standardized questionnaire. We conducted a receiver operating characteristic (ROC) curve analysis to determine the association strength and the clinically relevant cut-off point for variables presenting statistical significance for the area under the curve (AUC) (α = 0.05). An independent t-test was used to compare isokinetic variables between athletes with and without previous injury (α = 0.05). Results The results of the ROC curve analysis indicated that hamstring fatigue index values at 300o/s were associated with the presence of previous knee injury (area under the curve [AUC] = 73%, p= 0.004), and shoulder external rotators fatigue index values at 360°/s were not associated with the presence of previous shoulder injury (AUC = 68%, p= 0.053). Conclusions Elite volleyball athletes who reported previous knee injuries were prone to a higher fatigue index than those reporting no injuries. Knee flexor resistance training might be useful for those athletes who reported knee injuries in the previous season.

Resumo Objetivo O objetivo deste estudo é determinar se lesões prévias de ombro e joelho estavam associadas ao índice de fadiga isocinética e razão agonista/antagonista dos rotadores internos/externos do ombro e flexores/extensores do joelho em jogadores de voleibol. Métodos Esta é uma investigação transversal com 49 jogadores de voleibol de elite que competem em alto nível no Brasil. O índice de fadiga isocinética e os perfis de agonistas/antagonistas foram avaliados durante a pré-temporada. Além disso, para registro de lesões anteriores, os atletas responderam a um questionário padronizado. Conduzimos uma análise da curva de característica de operação do receptor (receiver operating characteristic, ROC) para determinar a força de associação e o ponto de corte clinicamente relevante de variáveis com significância estatística na área sob a curva (AUC) (α = 0,05). Um teste t independente comparou as variáveis isocinéticas entre atletas com e sem lesão prévia (α = 0,05). Resultados Os resultados da análise da curva ROC indicam que os valores do índice de fadiga dos isquiotibiais a 300o/s foram associados à presença de lesão prévia no joelho (área soba a curva [AUC] = 73%, p= 0,004), enquanto os valores do índice de fadiga dos rotadores externos do ombro a 360°/s não foram associados à presença de lesão prévia no ombro (AUC = 68%, p= 0.053). Conclusões Atletas de voleibol de elite que relataram lesões anteriores no joelho estavam propensos a um índice de fadiga maior do que aqueles que não relataram lesões. O treinamento de resistência de flexores do joelho pode ser útil para atletas com relatos de lesões no joelho na temporada anterior.

ABSTRACT. Technical and secure information on the production of quality atemoya seedlings (Annona squamosa L. x Annona cherimola Mill.) are of paramount importance due to the growing interest in this crop. This study aimed to assess the formation of atemoya seedlings propagated by grafting methods and cultivars using araticum (Annona sylvatica) rootstocks. The experiment was carried out in a screened environment at the Western Paraná State University (Unioeste), Campus of Marechal Cândido Rondon, Paraná State, Brazil. Atemoya grafts (Thompson, African Pride, and Gefner) were collected from plants from the CATI seedling nursery, located in São Bento do Sapucaí, São Paulo State, Brazil. Grafting was performed using 1-year-old araticum rootstocks with 8-10 mm in diameter at a height of 15 cm from the ground and grafts measuring, on average, 8.5 cm in length, 10 mm in diameter, and three buds. The experimental design consisted of randomized blocks in a 3 × 3 factorial arrangement (three grafting methods: cleft graft, whip and tongue graft, and chip budding × three cultivars: African Pride, Thompson, and Gefner), with four replications and 10 seedlings per replication, totaling 40 seedlings per treatment. The percentage of graft success (%), percentage of sprouting (%), number of sprouts, number of leaves, and longest sprout length (cm) were assessed at 60 days after grafting. The cleft graft method is efficient for producing atemoya seedlings. The cultivar African Pride can be grafted onto rootstocks by the cleft graft and whip and tongue graft methods, as they promote a higher percentage of graft success. The Annona sylvatica rootstock has potential for the production of atemoya seedlings, but chip budding is not viable.

RESUMO Para se obter um cultivo eficiente de sorgo granífero em sistemas de produção que utilizam água salina, torna-se necessário um manejo hídrico adequado para maximizar sua produção. Com isto, o presente estudo teve como objetivo avaliar o efeito da aplicação de frações de lixiviação no manejo da irrigação com água salina na produção de variedades de sorgo granífero e na distribuição de água e sais no perfil do solo, em condições semiáridas. O estudo foi realizado no município de Petrolina, região semiárida do Brasil. Foi utilizado o delineamento experimental em blocos casualizados, com quatro repetições, em parcelas subdivididas; sendo as parcelas quatro frações de lixiviação (FL): 0; 5; 10 e 15% com água salina proveniente de poço artesiano, as subparcelas três variedades de sorgo granífero: 1011-IPA, 2502-IPA e Ponta Negra e as subsubparcelas dois ciclos de cultivo (1º e 2º corte). As variáveis avaliadas foram: distribuição de água e sais no perfil do solo, variáveis biométricas, biomassa fresca, biomassa seca e produtividade de grãos. A aplicação de frações de lixiviação na irrigação com água salina promove melhor distribuição de sais no perfil do solo, com incrementos de até 60% no rendimento de grãos das variedades de sorgo avaliadas. A produção das variedades 1011-IPA e Ponta Negra é uma alternativa viável em sistemas irrigados com água salina com condutividade elétrica média de 4,19 dS m-1, em Argissolo Vermelho Amarelo, sob condições semiáridas.

ABSTRACT In order to obtain an efficient cultivation of grain sorghum in production systems that use saline water, an adequate management becomes necessary for maximizing its production. The present study aimed to evaluate the effect of the application of leaching fractions in the saline water irrigation management on the production of sorghum varieties and on the distribution of water and salts in the soil profile, under semiarid conditions. The study was carried out in the municipality of Petrolina, semiarid region of Brazil. The experimental design was randomized blocks, with four replicates, in split-split plots; four leaching fractions (LF): 0, 5, 10, and 15% with saline water from artesian well in the plots, three varieties of grain sorghum: 1011-IPA, 2502-IPA and Ponta Negra in the subplots, and two crop cycles (1st and 2nd cut) in the sub-subplots. The evaluated variables were distribution of water and salts in the soil profile, biometric variables, fresh biomass, dry biomass, and grain yield. Application of leaching fractions of up 15% in saline water irrigation promotes better distribution of salts in the soil profile, with increments of up to 60% in the grain yield of the sorghum varieties evaluated. The production of the varieties 1011-IPA and Ponta Negra is a feasible alternative in systems irrigated with saline water with average electrical conductivity of 4.19 dS m-1, in Ultisol, under semiarid conditions.

Resumen La Organización Mundial de la Salud ha declarado la enfermedad del nuevo coronavirus 2019 (COVID-19) una emergencia de salud pública mundial. A pesar de los síntomas respiratorios predominantes en COVID-19, la trombosis puede ocurrir en algunos pacientes, con un aumento de la morbimortalidad debido al empeoramiento respiratorio. Presentamos el caso de un hombre de 62 años con enfermedad similar a la gripe que fue diagnosticada como COVID-19 por RT-PCR de SARS-CoV-2. Después de tres semanas, de sarrolló dolor abdominal además de hinchazón, náuseas y vómitos. Fue sometido a laparotomía exploradora luego de que las pruebas de imagen sugirieran isquemia mesentérica. Se evidenció isquemia intestinal por ausencia de flujo en la arteria mesentérica superior y ramas yeyunales. Se realizó embolectomía y enterec tomía con evolución favorable, con mejoría clínica. Este caso añade datos a la limitada literatura sobre las complicaciones extrapulmonares del COVID-19, en particular las relacionadas con eventos tromboembólicos.

Abstract The World Health Organization has declared the novel coronavirus disease 2019 (COVID-19) a global public health emergency. Despite the predominating respiratory symptoms occurring in COVID-19, thrombosis can occur in some patients, with morbidity and mortality increase due to the respiratory worsening. This article reports the case of a 62-year-old man with a flu-like illness that was diagnosed as CO VID-19 by RT-PCR of SARS-CoV-2. After three weeks, he subsequently developed abdominal pain in addition to bloating, nausea, and vomiting. He underwent exploratory laparotomy after imaging tests suggested mesenteric ischemia. Intestinal ischemia was evident, due to the absence of flow in the superior mesenteric artery and jejunal branches. Embolectomy and enterectomy were performed and they resulted in a favorable outcome, with clinical improvement. This case adds data to the limited literature on extrapulmonary complications of COVID-19, notably those related to thromboembolic events.

Abstract Licania macrophylla is a medicinal plant from the Amazon. It is mainly used in the form of a decoction and has been reported to contain several phenolic compounds. However, the effect of seasonality on the phenolic composition and antioxidant potential of this plant has not been well studied, especially in the Amazon region, an area affected by the rainy and less-rainy seasons. Therefore, we evaluated the seasonality of these aromatic compounds and the antioxidant potential of the extracts from L. macrophylla stem bark. We also determined the correlation between the extraction methods used and precipitation levels during each period for 1 year. The total flavonoid and phenolic content, DPPH-scavenging potential, percentage of phosphomolybdenum complex reduction, and iron-reducing power were quantified. The levels of phenolic compounds were the highest in June, whereas those of flavonoids were the highest in September and October; however, these differences were not significant. The extracts from April, November, and June showed the best results for DPPH scavenging, phosphomolybdenum reduction, and iron reduction power, respectively. Significant differences in the phenolic content and DPPH-scavenging activity were observed between the more- and less-rainy seasons. The total phenolic content was positively correlated with FRAP and DPPH, whereas flavonoid levels were negatively correlated.

ABSTRACT The demand for specialty coffees in the national and international market grows and generates an excellent opportunity for the exploration of new business. The cup quality of coffee is defined by the genetic predisposition of varietal groups, effects of the production environment, crop management, and form of post-harvest processing. Genotypes of Yellow Bourbon have already shown a predisposition to the production of excellent cup quality, but it may differ in sensory attributes and cup quality. The aim of this article was to evaluate the agronomic and qualitative performance of Yellow Bourbon strains from the Active Germplasm Bank of the Agronomic Institute of Campinas, in order to identify the best genotypes for the production of specialty coffees. The experiment was conducted in São Sebastião da Grama, SP, Brazil, at 1,100 meters of altitude in a medium-textured Ultisol. Treatments consisted of 14 strains of Yellow Bourbon, arranged in a randomized block design with three replicates. Field, post-harvest and laboratory evaluations were carried out. The strains IAC J3-1, IAC J9-16 and IAC J19-18-10 stood out in terms of yield values and sensory quality above the average of the studied group. The strains IAC J9-16 and IAC J19-18-10 showed predominance of floral and honey nuances, respectively, indicating that they may have high potential to express these characteristics in the beverage. The results suggested that there are Yellow Bourbon strains suitable for production and with superior cup quality, which highlights the importance of the appropriate choice of genotypes for the production of differentiated specialty coffees.

RESUMO Objetivou-se neste estudo avaliar a influência da fração de lixiviação nas características biométricas, produtivas e na qualidade tecnológica do caldo de variedades de cana-de-açúcar cultivadas em solo salino no Semiárido brasileiro. O delineamento experimental foi em blocos casualizados, com três repetições, em esquema fatorial 2 × 3 × 3, sendo dois ciclos de cultivo da cana-de-açúcar: cana planta e cana soca; três variedades de cana-de-açúcar: RB72454, SP943206 e VAT90212; e, três frações de lixiviação da água de irrigação: 0; 9,1; e 16,6%. Avaliou-se número de folhas vivas, número de entrenós, área foliar, diâmetro do colmo, altura da planta, número de perfilhos, produtividade, teor de sólidos solúveis totais (°Brix), porcentagem de fibra industrial, pureza do caldo, Pol% do caldo, Pol% da cana e açúcar total recuperável. Ao final dos dois ciclos da cultura foi determinada a eficiência do uso de água. As variedades SP943206 e VAT90212 demonstraram maior produtividade sob fração de lixiviação da água de irrigação de 9,1% nos dois ciclos e maiores eficiência do uso de água foram observadas na variedade VAT90212. A aplicação de frações de lixiviação para reduzir a salinidade do solo não proporciona alterações na qualidade tecnológica das variedades RB72454, SP943206 e VAT90212 de cana-de-açúcar.

ABSTRACT The objective of this study was to evaluate the influence of leaching fraction on the biometric and production characteristics and technological quality of the juice of sugarcane varieties grown in saline soil in the Brazilian semiarid region. The experimental design was in randomized blocks, with three repetitions, in a 2 × 3 × 3 factorial scheme, corresponding to two sugarcane cultivation cycles: plant cane and ratoon cane; three sugarcane varieties: RB72454, SP943206 and VAT90212; and, three leaching fractions of irrigation water: 0; 9.1; and 16.6%. Number of living leaves, number of internodes, leaf area, stem diameter, plant height, number of tillers, yield, total soluble solids content (°Brix), percentage of industrial fiber, juice purity, juice Pol%, cane Pol% and total recoverable sugar were evaluated. At the end of the two crop cycles, water use efficiency was determined. The varieties SP943206 and VAT90212 showed higher yield under leaching fraction of irrigation water of 9.1% in both cycles, and higher water use efficiency values were observed for the variety VAT90212. Application of leaching fractions to reduce soil salinity does not promote changes in the technological quality of the sugarcane varieties RB72454, SP943206 and VAT90212.

Abstract Fig production and quality are associated with the number of branches per plant and may vary among regions according to the climatic conditions and crop management. In this work, we studied the influence of the number of branches per plant on the production and fruit quality of ‘Roxo de Valinhos’ figs under semiarid conditions. The experiment was carried out at the didactic orchard of Federal Rural University of Semiarid (UFERSA), Mossoró city, Rio Grande do Norte state, Brazil. Fig plants were grown with 6, 12, 18, or 30 branches in a completely randomized block design with four replicates and four plants per plot. Plants without thinning were also cultivated as controls. The number of fruits per plant, productivity, fruit length, fruit diameter, and fruit weight were evaluated. Additionally, fruit firmness, titratable acidity (TA), soluble solids content (SS), maturation index (SSC/TA), and vitamin C content were analyzed. The results showed that plants cultivated with 12, 18, and 24 branches produced fruits with higher weight, more fruits per branch, and more fruits per plant and thus were more productive. Additionally, fruits showed a high vitamin C content and maturation index.

Resumen La producción y calidad del higo están asociadas con el número de ramas por planta y pueden variar entre las regiones según las condiciones climáticas y el manejo del cultivo. En este trabajo se estudió la influencia del número de ramas por planta en la producción y calidad del fruto del higo ‘Roxo de Valinhos’ en condiciones semiáridas. El experimento se llevó a cabo en el huerto didáctico de la Universidad Federal Rural del Semiárido (UFERSA), ciudad de Mossoró, estado de Rio Grande do Norte, Brasil. Las plantas de higo se cultivaron con 6, 12, 18 o 30 ramas en un diseño de bloques completamente casualizados con cuatro repeticiones y cuatro plantas por parcela. También se cultivaron plantas sin eliminación de rama como tratamiento control. Se evaluó el número de frutos por planta, productividad, longitud, diámetro y peso del fruto. También se analizó la firmeza del fruto, acidez titulable (TA), contenido de sólidos solubles (SSC), índice de maduración (SSC / TA) y vitamina C. Los resultados mostraron que las plantas cultivadas con 12, 18 y 24 ramas produjeron frutos con mayor peso, número de frutos por rama, número de frutos por planta y, por tanto, fueron más productivas. Además, los frutos mostraron alto contenido de vitamina C e índice de maduración.

RESUMO A salinidade é um dos fatores que mais limita a produtividade agrícola no Semiárido brasileiro. Neste contexto, o presente estudo foi realizado com o objetivo de avaliar as trocas gasosas e as características biométricas de acessos do complexo Saccharum submetidas a estresse salino. O experimento foi realizado em casa de vegetação, instalada na sede da Embrapa Semiárido, em Petrolina, PE. O delineamento experimental foi em blocos casualizados, sendo os tratamentos representados por 19 acessos pertencentes a diferentes gêneros/espécies, sendo 10 acessos de Saccharum officinarum (BGCN 6, BGCN 91, BGCN 104, BGCN 127, BCGN 90, BGCN 101, BGCN 102, BGCN 118, BGCN 125 e BGCN 122), dois acessos de Saccharum spp. (BGCN 87 e BGCN 89), um acesso de Saccharum hybridum (BGCN 88), um acesso de Saccharum robustum (BGCN 94), quatro acessos de Erianthus arundinaceus (BGCN 117, BGCN 119, BGCN 120 e BGCN 123) e um acesso de Miscanthus spp., com três repetições. Foram avaliadas características biométricas, índice de clorofila e as trocas gasosas dos acessos submetidos à irrigação com água salinizada (6,0 dS m-1). Os acessos de E. arundinaceus (BGCN 120 e BGCN 123) apresentaram a maior taxa fotossintética, taxa de transpiração, altura de plantas e comprimento das folhas, indicando maior adaptabilidade ao estresse salino e podem ser promissores em programa de melhoramento.

ABSTRACT Salinity is one of the factors that most limit agricultural yield in the Brazilian semi-arid region. In this context, the present study aimed to evaluate the leaf gas exchange and biometric characteristics of accessions of the Saccharum complex subjected to salt stress. The experiment was carried out in a greenhouse, installed at Embrapa Semiárido, in Petrolina, PE, Brazil. The experimental design was in randomized blocks, with the treatments represented by 19 accessions belonging to different genera/species, being 10 accessions of Saccharum officinarum (BGCN 6, BGCN 91, BGCN 104, BGCN 127, BCGN 90, BGCN 101, BGCN 102, BGCN 118, BGCN 125 and BGCN 122), two accessions of Saccharum spp. (BGCN 87 and BGCN 89), one accession of Saccharum hybridum (BGCN 88), one accession of Saccharum robustum (BGCN 94), four accessions of Erianthus arundinaceus (BGCN 117, BGCN 119, BGCN 120 and BGCN 123) and one accession of Miscanthus spp., with three repetitions. Biometric characteristics, chlorophyll index and leaf gas exchange of the accessions were evaluated when they were subjected to irrigation with salinized water (6.0 dS m-1). E. arundinaceus accessions (BGCN 120 and BGCN 123) showed the highest photosynthetic rate, transpiration rate, plant height and leaf length, indicating greater adaptability to salt stress and could be promising in breeding programs.

Abstract Conyza species are important weeds in global agriculture, especially due to their capacity to evolve resistance to multiple herbicide mechanisms of action. We aimed to evaluate the frequency and distribution of resistance to glyphosate and chlorimuron-ethyl in Conyza spp. populations from Brazil. Seed samples were collected from grain production areas across nine Brazilian states over five consecutive years (2014 to 2018). Prior to resistance monitoring trials, dose-response assays were conducted to determine a single dose of glyphosate or chlorimuron-ethyl to discriminate resistant and susceptible populations. Resistance monitoring based on plant responses to the application of discriminatory doses of glyphosate (960 g ha-1) or chlorimuron-ethyl (20 g ha-1). Populations were classified as resistant, moderately resistant, or susceptible to either herbicide. While glyphosate resistance was highly frequent (71.2%) in all the five years, chlorimuron-ethyl resistant populations occurred at 39.8% of the total. The frequency of multiple resistance to both herbicides (35.3%) was proportional to the occurrence of chlorimuron-ethyl resistance (39.6%). Resistance to glyphosate and to chlorimuron-ethyl were found across all states evaluated.

Abstract A thermal comfort index for the Northeast of Brazil was analyzed for two scenarios of climatic changes, A1B and A2, for 2021-2080, and compared with the reference period 1961-1990. A technique of regionalization was applied to rainfall, maximum and minimum temperature data from meteorological stations, obtained by statistical downscaling of projections from four global climate models. The results pointed to a significant reduction of rainfall and an increase of temperature for three different climatically homogeneous subregions. Regarding the thermal comfort index, the results point to an increase in days with heat discomfort between 2021 and 2080. In the northern portion, the higher percentage of days with heat discomfort will be significant since the first half of the period under appreciation, i.e., from 2021 to 2050. Conversely, in the eastern of northeastern Brazil, the increase of days with heat discomfort should happen in the period from 2051 to 2080, whereas the central-western part of the region, which, in the reference period, had recorded less than 1% of days with heat discomfort, might see an elevation of that percentage to 7% between 2021 and 2050, potentially reaching 48% of its days made uncomfortable by heat between 2051 and 2080.

Resumo: As bromélias são numerosas em diversidade e abundância na Mata Atlântica, um dos biomas mais ameaçados do planeta. Elas também são um habitat importante para várias espécies de animais que vivem na água retida por sua estrutura em roseta. Neste estudo, objetivamos caracterizar e comparar a comunidade de macroinvertebrados de duas espécies de bromélias de fragmentos de Mata Atlântica. Uma é característica de Floresta Ombrófila Densa, Aechmea ornata, e a outra ocorre em Floresta Ombrófila Mista, Aechmea recurvata. Amostramos e identificamos macroinvertebrados de 24 bromélias, 12 de cada espécie. No total foram registrados 19 táxons e 656 espécimes. No geral, Diptera foi a ordem de inseto mais abundante (n = 417), representada principalmente por culicídeos (n = 227) e quironomídeos (n = 107), seguidos por besouros Scirtidae (n = 216). Comparativamente a A. recurvata, A. ornata apresentou maior abundância e menor riqueza de invertebrados. Sete táxons foram exclusivos de A. recurvata, oito foram comuns a ambas e quatro, incluindo uma esponja, ocorreram apenas em A. ornata. Até onde sabemos, este é o primeiro registro de esponjas em bromélias. Observamos que os táxons de invertebrados mais abundantes eram comuns a ambas as bromélias e são considerados típicos de ambientes lênticos. Por outro lado, o elevado número de táxons exclusivos indica que os esforços de conservação devem incluir bromélias dessas duas fitofisionomias para garantir a diversidade das comunidades de fitotelmatas.

Abstract: Bromeliads are numerous both in diversity and abundance in the Atlantic Rainforest, one of the most threatened biomes on Earth. They are also an important habitat for several animal species that live in the water retained by its rosette structure. In this study, we aimed to characterize and compare the macroinvertebrate community of two bromeliad species from Atlantic forest fragments. One is characteristic of Dense Ombrophilous Forests, Aechmea ornata, and the other occurs in Mixed Ombrophilous Forests, Aechmea recurvata. We sampled and identified macroinvertebrates form 24 bromeliads, 12 of each species. A total of 19 taxa and 656 specimens were sampled. In both bromeliad species, Diptera was the most abundant insect order (n=417), represented mostly by mosquitoes (n=227) and non-biting midges (n=107), followed by Scirtidae marsh beetles (n=216). Aechmea ornata presented higher abundance and lower invertebrate richness compared to Aechmea recurvata. Seven taxa were exclusive of A. recurvata, eight were common to both, and four, including a sponge, occurred only in A. ornata. The most abundant invertebrate taxa were common to both bromeliads and are regarded as typical of lentic environments. On the other hand, the high number of exclusive species indicates that conservation efforts should include bromeliads from these two phytophysiognomies to secure protecting the diversity of phytotelmata communities.

ABSTRACT Jatropha curcas L. (Euphorbiaceae) is a plant species widely present in tropical and subtropical regions that have been studied mainly for its potential for biofuel production; it is also a plant known for its toxic substances. To explore its use as an insecticide, we studied the effect of the seed extract from green fruit and the seed oils from the mature and dried fruit of J. curcas on the green aphid Myzus persicae (Hemiptera: Aphididae). Seven concentrations of extracts and oils of different maturation stages of fruits were used and sprayed with the Potter Spray Tower on cabbage leaves containing 10 individuals of M. persicae. Ten repetitions for each concentration were carried out at each stage of maturation (green, ripe, and dry), and the evaluation of the mortality of individuals occurred for a period of 72 hours. The maturation stage of J. curcas, as well as the concentration and time after the application, influenced the mortality of M. persicae. The best results were obtained 48h after applying the dried fruit oil at 2.5% and 72 hours after the application of dry and ripe fruit oil at 2.5%.

RESUMEN La Jatropha curcas L. (Euphorbiaceae) es una especie vegetal, ampliamente presente en regiones tropicales y subtropicales, que ha sido estudiada principalmente por su potencial para la producción de biocombustibles. Pero también es una planta conocida por sus sustancias tóxicas. Para verificar su uso como insecticida, estudiamos el efecto del extracto de semillas de frutos verdes y aceites de semillas de frutos maduros y secos de J. curcas sobre el pulgón verde Myzus persicae (Hemiptera: Aphididae). Siete soluciones, en diferentes concentraciones de los extractos y aceites, fueron utilizadas y pulverizadas con la Torre de Pulverización de Potter en hojas de repollo, con 10 individuos de M. persicae cada una. Se realizaron diez repeticiones para cada concentración en cada etapa de maduración (verde, maduro y seco) y la evaluación de la mortalidad de los individuos ocurrió en un período de 72 horas. El estadio de maduración de J. curcas, la concentración y el tiempo después de la aplicación influyeron la mortalidad de M. persicae. Los mejores resultados se obtuvieron 48 horas después de la aplicación del aceite de frutos secos a 2,5%, y 72 horas después del aceite de los frutos secos y maduros a 2,5%.