Abstract Ruminant energy supplementation with vegetable oils or fats has been standing out worldwide and oil palm processing has been receiving growing interest. This study assessed the effect of supplementation with saturated and unsaturated fatty acids from the palm oil industry on the lipid profile of seminal plasma and of the sperm membrane, as well as on the morphological and functional characteristics of raw and cryopreserved buffalo semen. Twelve purebred Murrah bulls (Bubalus bubalis) were assigned to the experimental groups and fed diets for 120 days with no added lipids (CONT, four bulls), or with an extra amount of 3% lipids from crude palm oil (PALM, four bulls), or from palm oil deodorizer distillate (PODD, four bulls). Semen was collected and cryopreserved every 15 days. The lipid composition of membranes and semen quality were determined after collections. Lipid supplementation did not impact feed intake (P>0.05). Diet enrichment with PALM increased the linoleic acid (C18:2,ω6) in seminal plasma. Lipid supplementation did not increase the polyunsaturated fatty acids in the sperm membrane composition, but significantly increased the lignoceric acid (C24:0). Cryopreserved semen of the supplemented bulls presented higher progressive motility (60.2 vs. 67.9 vs. 65.2%; P<0.05) and sperm viability detected by eosin-nigrosin staining (61.1 vs. 69.4 vs. 67.8%; P<0.05). Palm oil reduced major sperm defects in both raw (12.2 vs. 9.3 vs. 13.2%; P<0.0001) and cryopreserved semen (12.4 vs. 9.4 vs. 11.2%; P<0.0001). The lipids added to the diet did not impact the population of spermatozoa with intact plasma and acrosomal membranes (PI-/PSA-), but significantly increased the percentage of spermatozoa with high mitochondrial potential (25.6 vs. 31.5 vs. 32.0%; P=0.008). The results suggest that lipid supplementation based on crude palm oil or palm oil deodorizer distillate can be safely used to feed buffalo bulls and may increase sperm attributes related to male fertility.

RESUMO O objetivo foi avaliar a influência de três alturas pré-pastejo do capim Massai sobre o comportamento ingestivo de ovinos sob lotação rotativa. Foram utilizados 18 ovinos mestiços Dorper x sem padrão racial definido (SPRD) sob lotação rotativa em capim Massai nas alturas pré-pastejo de 45, 35 e 25 cm. A lotação animal foi estimada para garantir a redução de aproximadamente 50% da altura inicial. As avaliações de comportamento foram feitas das 6 às 18 h na entrada e na saída dos animais dos piquetes. A maior atividade de forma geral realizada foi a de pastejo, seguida de ruminação e outras atividades, sendo o período de 6 às 10 h o que apresentou maior atividade de pastejo. Nos demais períodos, os animais no tratamento com 35 cm pastejaram menos que os animais dos outros tratamentos. A ruminação, em todos os tratamentos, foi mais significativa no período das 14 às 18 h, mas os animais no tratamento com 45 cm foram os que passaram maior tempo realizando esta atividade. Os animais no tratamento com 45 cm apresentaram maior tempo de ruminação do bolo alimentar e maior número de mastigações merícicas. A taxa de bocados foi superior para o tratamento com 25 cm, seguido do com 45 e 35 cm respectivamente.

SUMMARY This studyaimed to assess the influence of three pre-grazing heights of Massai grass on the ingestive behavior of sheep under rotational stocking. Eighteen Dorper x nondescript crossbred sheep were used under rotational stocking on Massai grass at pre-grazing heights. The animal stocking was estimated to ensure a reduction of approximately 50% of the initial height. Behavior assessments were performed from 6 to 18 h at the entrance and exit of animals from paddocks. The longest activity was grazing, followed by rumination and other activities, and the period from 6 to 10 h showed the longest grazing.In the other periods, the animals in the 35-cm treatment grazed less when compared to those ofthe other treatments. Rumination in all treatments was more significant in the period from 14 to 18 h, but the animals in the 45-cm treatment spent more time doing this activity, which gave them a higher time of rumination of the food bolus, as well as ruminating chews. The bite rate was higher for the 25-cm treatment, followed by the 45 and 35-cm treatments, respectively.

ABSTRACT The ability of discriminating carcass characteristics of different fat cover scores of heavy carcasses, according to the European (EUS) and Brazilian (BRS) classification systems, was assessed. Fifty-six lambs, weighing between 26.25 and 46.15 kg, of the Santa Inês and crossbred Santa Inês × Dorper genetic groups were evaluated. The level of adiposity was assessed through color photography of the carcasses after refrigeration according to the EUS and BRS. The carcasses were assigned to four groups by cluster analysis according to 25 variables, namely, cold carcass weight; muscle; bone; fat; and muscle:fat and fat:bone ratios of the carcass and cuts (hindquarter, shoulder, back); kidney, pelvic, and inguinal fat; and subcutaneous fat thickness. Of the four groups obtained by cluster analysis, the scores according to the different classification systems only statistically differed between groups 1 and 4. The BRS had a higher number of variables well correlated with the scores by assessors than the groups classified by the EUS. The BRS was better correlated with tissue composition. However, most variables were better correlated with backfat thickness than the score obtained through the classification systems. Better results were obtained regarding the prediction of carcass fat by the BRS using backfat thickness or cold carcass weight. The Brazilian lamb carcass classification system better predicts tissue composition and is the best method to discriminate intermediate-fat classes when associated with cold carcass weight.

Objetivou-se com o experimento demonstrar a composição bromatológica e avaliar a cinética de fermentação e degradabilidade ruminal efetiva in vitro, por meio da técnica "Hohenheim Gas Test", da farinha do mesocarpo do babaçu I® e II® (FMBI e FMBII) e de rações com diferentes níveis de inclusão. Foram conduzidos dois ensaios: um com os subprodutos puros e milho e outro com rações com inclusões da FMBI e FMBII nas seguintes proporções: 0; 7,5; 15,0 e 22,5% da matéria seca total. O delineamento foi o de blocos casualizados sendo seis blocos no primeiro e cinco no segundo ensaio. Os tempos de mensuração dos gases produzidos foram 3; 6; 9; 12; 24; 48; 72 e 96 horas. As farinhas FMBI e FMBII apresentaram composições bromatológicas diferentes, em relação aos constituintes da fibra (FDN, FDA e Lignina). As farinhas FMBI e FMBII apresentaram padrões de cinética de fermentação inferiores aos padrões de fermentação do milho grão. Com o aumento da proporção de FMBI e FMBII na ração, reduz-se a produção de gases por tempo de incubação. Em virtude do exposto, pode-se afirmar que as farinhas do mesocarpo I e II, apesar de serem fontes de amido, não apresentaram fermentação ruminal semelhante à fonte amilácea padrão, o grão de milho.

The objective of the experiment was to demonstrate the bromatological composition, and evaluate the fermentation kinetics and effective ruminal degradability in vitro, using the "Hohenheim Gas Test" technique, of babassu mesocarp flour I® and II® (FMBI and FMBII) and of feeds at different levels of inclusion. Two trials were carried out: one of pure byproducts and corn, and another of feeds with inclusions of FMBI and FMBII in the following proportions: 0, 7.5, 15.0 and 22.5% of the total dry matter. The experimental design was of randomized blocks, with six blocks in the first trial and five in the second. Measurement times for the gases produced were 3, 6, 9, 12, 24, 48, 72 and 96 hours. The FMBI and FMBII flours showed different bromatological composition for the fibre components (FDN, FDA and Lignin). The FMBI and FMBII flours also showed patterns for fermentation kinetics inferior to the fermentation patterns of corn grain. Increasing the ratio of FMBI and FMBII in the feed reduces gas production for time of incubation. In view of the above, it can be said that mesocarp flours I and II, despite being sources of starch, did not present ruminal fermentation similar to corn grain, the standard source of starch.

O objetivo do estudo foi conhecer a prevalência sorológica de Toxoplasma gondii em búfalos (Bubalus bubalis) do Estado do Pará, Brasil. Foram selecionados randomicamente 319 bubalinos distribuídos em sete municípios da Ilha do Marajó. Para efeito comparativo também foram avaliados 128 bubalinos pertencentes a cinco municípios do Estado do Pará. A prevalência sorológica de Toxoplasma gondii foi avaliada pelo Ensaio de Imunoadsorção Enzimático Indireto (iELISA). As amostras diagnósticadas como positivas no iELISA foram submetidas a Reação de Imunofluorescência Indireta (RIFI). Foram avaliados os fatores de risco: localidade, raça, gestação, co-infecção por Brucella abortus e co-infecção por Mycobacterium bovis. As frequências de animais positivos no iELISA para T. gondii foram comparadas pelo teste de Qui-quadrado (χ2) com 95% de confiabilidade. As variáveis com p<0,2 foram submetidos à análise de regressão logística, sendo o modelo construído baseado no teste da "odds ratios". A prevalência de T. gondii observada no iELISA foi de 41,6% (186/447). Na RIFI, 86,5% (161/186) das amostram positivas no iELISA tiveram sua positividade para T. gondii confirmada. A prevalência média nos municípios da Ilha do Marajo e do Continente foi de 32% (103/319) e 55% (70/128), respectivamente. Os municípios que apresentaram as maiores prevalências foram Soure (53%) e Salvaterra (49%) na Ilha do Marajó e Castanhal (55%) e Tailândia (50%) no Continente. Os fatores de risco raça e co-infecção por Brucella abortus ou Mycobacterium bovis não influenciaram na prevalência de T. gondii. Além disso, animais gestantes foram 57% mais positivos para T. gondii do que animais não gestantes. A circulação de anticorpos é um indicativo da presença do agente da toxoplasmose em búfalos no Estado do Pará. Esses achados representam um risco não apenas para os animais de produção, mas à saúde pública, como uma fonte de infecção.

The aim was to study the seroprevalence of Toxoplasma gondii in water buffaloes (Bubalus bubalis) from State of Pará, Brazil. Three hundred and nineteen buffaloes were randomly selected into seven municipalities of Marajó Island. For comparative purposes, 128 buffaloes of five municipalities in the state of Pará were also evaluated. The seroprevalence of T. gondii was evaluated by Indirect Enzyme Linked Immunosorbent Assay (iELISA). The samples diagnosed as positive in iELISA were subjected to Immunofluorescence Antibody Test (IFAT). We evaluated risk factors: location, breed, pregnancy and co-infection with Brucella abortus or Mycobacterium bovis. The frequency of animals positive for T. gondii in iELISA were compared by chi-square (χ2) with 95% confidence. Variables with p <0.2 were subjected to logistic regression analysis; the model was built based on the "odds ratios" test. The prevalence of T. gondii in iELISA was 41,6% (186/447). In IFAT, 86,5% (161/186) had their positivity for T. gondii confirmed. The average prevalence in the municipalities of the Marajó Island and of the mainland was 32% (103/319) and 55% (70/128), respectively. The municipalities with the highest prevalence were Soure (53%) and Salvaterra (49%) in Marajó Island, and Castanhal (55%) and Thailândia (50%) in the Continent. The breed and co-infection with Brucella abortus or Mycobacterium bovis presented no influence on the prevalence of T. gondii. Additionally, pregnant animals were 57% more positive for T. gondii than nonpregnant animals. The presence of antibodies is an indicative of T. gondii in buffaloes in the state of Pará, and these findings represent a risk not only for farm animals, but to public health as a source of infection.

The objective of this study was to evaluate the effect of inclusion of lipid residue of biodiesel originated in the processing of palm oil (Elaeis guineensis) in the diet on the digestibility of feedlot lambs. Twenty-five crossbred male castrated lambs, weighing 20±1.61 kg, were distributed in randomized blocks with five treatments and five replications. The experimental period lasted 22 days; 15 for diet adaptation, 2 for the adaptation to the indicator LIPE (lignin from Eucalyptus grandis isolated, purified and enriched, UFMG, Minas Gerais) and 5 for fecal sampling. Diets were formulated with 64% concentrate based on corn and soybean meal, 31% Massai grass (Panicum maximum cv. Massai) hay and 5% lipid supplementation from increasing levels of substitution of 0, 25, 50, 75 and 100% of palm oil for biodiesel oil from palm residue. The lambs were offered two meals a day, at 7h00 and 16h00. There was linear effect of inclusion of the residue from palm oil biodiesel on dry matter intake. There was no change in digestibility of nutrients except for ether extract. The use of biodiesel from palm oil residue up to 100% replacement for the lipid supplementation of sheep positively influences the consumption without altering the digestibility of nutrients.

Avaliou-se o efeito de quatro tamanhos de partícula do volumoso e de dois manejos alimentares sobre o consumo, a digestibilidade aparente e o comportamento ingestivo em ovinos. Utilizaram-se 24 carneiros com peso vivo médio de 37,0kg, alimentados com relação volumoso:concentrado (V:C) 25:75%. Foi utilizado o feno de Tifton-85 como volumoso e um concentrado comercial. Adotou-se esquema fatorial 2x4 em delineamento inteiramente ao acaso (dois manejos de alimentação e quatro tamanhos de partícula) o que totalizou 6 animais por tratamento. Os tratamentos não afetaram as variáveis referentes ao consumo e à digestibilidade aparente. O consumo médio de matéria seca foi de 97,43g/dia/UTM e a digestibilidade aparente da matéria seca média observada foi de 66,12%. O consumo de fibra em detergente neutro foi de 47,22g/dia/UTM. O menor tamanho de partícula (2mm) apresentou os menores tempos gastos (minutos/dia) com a ruminação (214,58) e com a atividade mastigatória total (360,41). Os animais gastaram 423,48min/dia/kg do consumo de fibra em detergente neutro ruminando e 676,19min/dia/kg do consumo de fibra em detergente neutro com atividade mastigatória total. Os tempos gastos com a ruminação e com a atividade mastigatória total em ovinos são diminuídos com a redução do tamanho de partícula do volumoso. Os tamanhos de partícula do volumoso e a frequência de fornecimento da dieta não afetam o consumo e a digestibilidade aparente dos nutrientes em ovinos.

Intake, apparent digestibility and ingestive behavior were studied in sheep fed four particle size of forage in two daily frequencies. Twenty-four sheep with live weight of 37.0kg were studied in a completely randomized design in factorial 2x4 scheme (two feeding frequency and 4 particle sizes) totaling six animals per treatment. Tifton-85 hay and a commercial concentrate were used with a ratio of 25:75%, respectively. The responses of intake and apparent digestibility were not modified with the treatments. Dry matter intake (DMI) was 97.43g/day/kgW0.75 and DMDA was 66.12%. The intake of neutral detergent fiber was 47.2g/day/kgW0.75. Smaller particle size (2mm) had lower times of rumination (214.58minutes/day) and chewing activity (360.41minutes/day). Animals spent 423.48minutes/day/kg of intake of neutral detergent fiber in rumination and 676.19 minutes/day/kg of intake of neutral detergent fiber in chewing activity. The times spent with rumination and the total chewing activity in sheep decreased as the particle size of the forage was reduced. The particle size of the forage and frequency of feeding did not affect the intake and apparent digestibility in sheep.

Foi estudada uma doença em 159 ovinos em 15 propriedades localizadas em sete municípios das mesorregiões nordeste e sudeste do estado do Pará e uma em um município do estado de Roraima. Os ovinos da raça Santa Inês e seus mestiços, de ambos os sexos e com dois a quatro anos de idade, eram os mais acometidos. A doença caracterizava-se por alopecia em diversas regiões do corpo, principalmente ao redor dos olhos; essas lesões evoluíam para eritemas multifocais, pequenas pápulas e crostas. Os animais apresentavam prurido intenso no local das lesões, inquietação, perda de peso e corrimento ocular. Sempre era observada a presença de mosquitos ao redor dos animais. Com o auxílio de um puçá foi realizada a captura de insetos associados às lesões, durante o repasto nos ovinos, para a identificação. Foram identificados insetos dos gêneros Simulium e Hippelates. Realizou-se biópsia da pele lesada de 10 ovinos. As lesões histológicas se caracterizaram por leves infiltrados inflamatórios mononucleares na derme, com presença de eosinófilos. Os estudos epidemiológicos, clínicos e patológicos desses casos, bem como o descarte dos diagnósticos diferenciais, permitiram concluir que se trata de dermatite alérgica por picada de insetos.

A skin disease was studied in 159 sheep on 15 farms located in seven counties of northeastern and southeastern of the state of Pará and in one farm in the state of Roraima, Brazil. The disease affected mainly two to four year-old sheep of the Santa Ines breed and crossbreds. Clinical signs were characterized by alopecia, multifocal erythema, small papules, and crusts in several areas of the body, mainly around the eyes. Severe itching in the affected areas, restless, weight loss and lacrimation were also observed. Insects were always seen around the animals. Insects apparently associated with the lesions were captured and identified as pertaining to the genera Simulium and Hippelates. Biopsies of the skin lesions of 10 sheep revealed inflammatory non suppurative mononuclear infiltrates of the dermis with presence of eosinophils. The epidemiologic, clinical and pathological studies of the cases allowed the diagnosis of allergic dermatitis due to the insect bites.

O objetivo do estudo foi avaliar o desempenho e as características da carcaça e da carne de novilhos não-castrados alimentados com ou sem adição de monensina (M) e/ou probiótico (P) (Sacharomyces cerevisiae) à dieta. Os animais foram distribuídos em baias individuais, permanecendo 145 dias em confinamento. A dieta foi composta de silagem de milho e 1,2% do peso vivo de concentrado com base da matéria natural. Não houve efeito da adição dos aditivos fornecidos de forma isolada ou mesmo da associação destes sobre o consumo de alimento, o ganho de peso e a conversão alimentar. As médias de consumo, ganho de peso e conversão alimentar apresentaram comportamento quadrático com o avanço do período de confinamento. Embora a adição de M ou P à dieta tenha resultado no aumento numérico (P>0,05) do consumo (4,2%), a média de ganho de peso reduziu (5,8 e 5,3%, respectivamente), resultando em pior conversão alimentar (P>0,05). Já a associação de M+P aumentou (P>0,05) o consumo em 9,5%, com concomitante aumento (P>0,05) do ganho de peso (6,4%) em relação à dieta controle. Os animais alimentados com M+P apresentaram melhor acabamento de carcaça (5,5mm), seguidos por aqueles do grupo controle (4,7mm), sendo os valores inferiores verificados nas carcaças dos animais M (3,7mm) e P (3,5mm). A adição de monensina e/ou probiótico (Sacharomyces cerevisiae) na dieta de novilhos na fase de terminação em confinamento não proporciona melhora no desempenho e nas características da carcaça e da carne de novilhos.

The objective of this experiment was to evaluate the performance, carcass and meat characteristics of non-castrated cattle with or without monensin (M) and/or probiotic (P) (Saccharomyces cerevisiae) to the diet. The animals were divided into individual stalls, being left 145 days in feedlot. The roughage used was corn silage + 1.2% of the weight live of concentrate in natural matter basis. There was no effect of the addition of the addictives supplied isolated or associated over feed intake, weight gain and fed conversion. Feed intake, weight gain and feed conversion averages presented quadratic behavior with the increase of the period feedlot. Although the addition of M or P to the diet resulted in the numeric increase (P>.05) of the intake (4.2%) the average of weight gain reduced (5.8 and 5.3%, respectively), resulting in smaller fed conversion (P>.05). The association of M+P already increased (P>.05) the food intake to the order of 9.5% with concomitant increase (P>.05) of the weight gain (6,4%) when compared to diet control. The animals fed with M+P presented better carcass finish (5.5mm), followed by the control (4.7mm), and smaller values, it was verified in the carcasses of the animals that received M (3.7mm) and P (3.5mm). The addition of monensin and/or probiotic for feedlot steers did not improve the performance, carcass and meat quality.