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This study describes the validation of a headspace gas chromatography with flame ionization (HS-GC-FID) method for the determination of propanal, pentanal, hexanal, octanal, and trans-2-nonenal originated by lipid oxidation from cat food samples, as well as the evaluation of the matrix effect in the results. This method was applied to the analysis of commercial cat food and proved to be suitable for the determination of these volatile compounds in different samples. Mean recoveries between 88 and 109% were obtained and repeatability expressed as relative standard deviation was always lower than 6.95%. The intra- and inter-assay precisions ranged from 0.44 to 20.88% and from 0.45 to 20.52%, respectively. In addition, the matrix effect of cat food samples was determined by comparing the slopes of the standard addition method, and the external calibration curve and its influences were verified. These results highlight the high potential of this method, which allows the determination of lipid oxidation products in cat food samples directly, without requiring prior sample preparation techniques.

https://doi.org/10.21577/0103-5053.20230026

Fruit production and quality of ‘Paluma’ guava with nematode-tolerant rootstock irrigated in the semi-arid region
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Simões, Welson L.

; Andrade, Victor P. M. de

; Silva, Jucicléia S. da

; Santos, Carlos A. F.

; Sousa, José S. C. de

; Calgaro, Marcelo

; Barbosa, Kaio V. F.

; Nascimento, Bruno R. do

Revista Brasileira de Engenharia Agrícola e Ambiental

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Mai 2023, Volume 27 Nº 5 Páginas 400 - 406

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Using nematode-resistant and/or -tolerant rootstock is a strategy that has maintained guava cultivation feasible in the irrigated perimeters of the Brazilian semi-arid region. The objective was to evaluate the production, gas exchange, and fruit quality of ‘Paluma’ guava with and without nematode-tolerant rootstock under different irrigation depths and production periods. The experimental design was randomized blocks, in the mixed 2 × 4 × 2 factorial scheme, corresponding to two forms of cultivation of ‘Paluma’ guava (without and with nematode-tolerant rootstock), four irrigation depths (60, 80, 100, and 120% of crop evapotranspiration - ETc), and two production periods, with four replicates. Guava plants were evaluated for physiological, production and post-harvest quality parameters. Gas exchange in ‘Paluma’ guava leaves was higher in the second production cycle, under irrigation depths above 100% ETc, not differing with the use of the rootstock. The use of the rootstock ‘BRS Guaraçá’ promotes a greater number of fruits and production with the increase of the irrigation depth, mainly in the second half of the year, with the increase of soluble solids and titratable acidity in the fruits.

RESUMO A utilização de porta enxerto resistente e ou tolerante a nematoides é uma estratégia que tem tornado viável a manutenção dos cultivos de goiabeira nos perímetros irrigados do semiárido brasileiro. Assim, o objetivo foi avaliar a produção, trocas gasosas e qualidade dos frutos da goiabeira ‘Paluma’ com e sem o porta enxerto tolerante a nematoide sob diferentes lâminas de irrigação e épocas de produção no Semiárido Brasileiro. O delineamento experimental foi em blocos ao acaso, no esquema fatorial misto 2 × 4 × 2, sendo duas formas de cultivo da goiabeira ‘Paluma’ (sem e com o porta enxerto tolerante a nematoide), quatro lâminas de irrigação (60, 80, 100 e 120% da evapotranspiração da cultura - ETc) e duas épocas de produção, com quatro repetições. Foram realizadas avaliações fisiológicas das plantas, produção e qualidade pós-colheita. As trocas gasosas nas folhas da goiabeira ‘Paluma’ foram maiores no segundo ciclo de produção, quando irrigada com lâminas de irrigação acima de 100% da ETc, não diferindo com o uso do porta enxerto. O uso do porta-enxerto ‘BRS Guaraçá’ proporciona um maior número de frutos e produção com o aumento da lâmina de irrigação, principalmente no segundo semestre do ano, bem como o aumento do teor de sólidos solúveis e acidez nos frutos.

https://doi.org/10.1590/1807-1929/agriambi.v27n5p400-406

Cut off Behavior of Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) in Soybean (Glycine max (L.) Merrill) Seedlings
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Araújo, Waldenio Antonio de

; Degrande, Paulo Eduardo

; Malaquias, José Bruno

; Silvie, Pierre Jean

; Scoton, Ana Maria Nascimento

; Pachú, Jéssica Karina da Silva

Brazilian Archives of Biology and Technology

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2023, Volume 66 elocation e23220386

Resumo: En | Texto: En | PDF: En

Abstract In addition to destroying the leaves, stems, pods, and grains of soybean (Glycine max), Spodoptera frugiperda larvae may also have the cut off behavior in the seedlings close to the ground, harming the establishment of the soybean crop. Therefore, this study aimed to evaluate the consequences of the fall armyworm attack by adopting the cut off behavior, which has been similarly documented for black cutworm (Agrotis ipsilon) in newly emerged soybean plants. The treatments were five levels of infestation with 0, 5, 10, 20 and 40 larvae per m2. Three variables were assessed: (1) stand (relationship between attacked and initial number of plants), (2) types and amounts of injured structures, such as hypocotyl (embryonic shoot), and the cotyledons (seed leaves), and (3) level of defoliation. The variables were collected every 24 hours until the pupal stage. Additionally, the final stand of the seedlings was quantified, with the highest injury intensity observed in the plots that contained the highest number of larvae. In the second experiment, the dynamics of movement and attacks of plants by S. frugiperda larvae at the following times of the day: 07:00, 10:00, 13:00, 16:00, 19:00 and 21:00 were analyzed with supervised machine learning models. The injury caused by S. frugiperda with the behavior of the cut off and the voracity of the larvae were evident in the structures (hypocotyl and cotyledons) of the evaluated plants. The results of the present study emphasize the need to manage S. frugiperda during the preplanting phase of soybean.

https://doi.org/10.1590/1678-4324-2023220386

Genetic diversity of the species Cnesterodon hypselurus (Cyprinodontiformes: Poeciliidae) in Cinzas River basin: new record and headwater capture evidences
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Ferreira, Dhiego G.

; Galindo, Bruno A.

; Souza, Tais C. de

; Pereira, Leonardo B.

; Bernardes, Victor A. P.

; Marques, Ana J. C.

; Frantine-Silva, Wilson

; Kotelok-Diniz, Thais

; Aggio, Carlos E. G.

; Apolinário-Silva, Caroline

; Zanatta, Augusto S.

; Sofia, Silvia H.

Neotropical Ichthyology

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2023, Volume 21 Nº 1 elocation e230007

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Cnesterodon hypselurus is a small fish that has a restricted distribution in southern Brazil, including headwaters of the Tibagi and Itararé river basins (Upper Paraná River). This study reported C. hypselurus in a headwater of Cinzas River basin, where there were no previous records of this species, and employed microsatellite loci and mitochondrial haplotypes in a population genetic analysis. A total of 57 specimens was analyzed, including 30 from Cinzas River basin, 25 from Itararé River basin and two from Tibagi River basin. Results indicated low genetic diversity levels (HE = 0.334 and h = 0.246) for the sample from Cinzas River, suggesting reflections of a founder effect after the species had dispersed from one watershed to another, possibly by headwater captures. Since different populations were detected between the Cinzas and Itararé rivers (DEST = 0.248, P-value < 0.05) and other occurrence sites are still unknown in the Cinzas River basin, the data herein have great relevance and should be taken into account in future management and conservation actions, as well as in evolutionary studies of C. hypselurus.

Resumo Cnesterodon hypselurus é um pequeno peixe que possui distribuição restrita no sul do Brasil, incluindo cabeceiras das bacias dos rios Tibagi e Itararé (alto rio Paraná). Este estudo reportou C. hypselurus na cabeceira da bacia do rio das Cinzas, onde não havia registros prévios desta espécie, e empregou locos microssatélites e haplótipos mitocondriais em uma análise genética de populações. Um total de 57 espécimes foi analisado, incluindo 30 do rio das Cinzas, 25 da bacia do rio Itararé e dois da bacia do rio Tibagi. Os resultados indicaram baixos níveis de diversidade genética (HE = 0,334 e h = 0,246) para a amostra do rio das Cinzas, sugerindo reflexos de um efeito fundador após a espécie ter dispersado de uma bacia para a outra, possivelmente a partir de captura de cabeceiras. Uma vez que diferentes populações foram detectadas entre os rios das Cinzas e Itararé (DEST = 0,248, valor de P < 0,05) e que outros pontos de ocorrência ainda são desconhecidos na bacia do rio das Cinzas, os dados do presente estudo mostram grande relevância e deveriam ser considerados em futuras ações de manejo e conservação, bem como em estudos evolutivos de C. hypselurus.

https://doi.org/10.1590/1982-0224-2023-0007

Diretriz Brasileira de Dispositivos Cardíacos Eletrônicos Implantáveis – 2023
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Teixeira, Ricardo Alkmim

; Fagundes, Alexsandro Alves

; Baggio Junior, José Mário

; Oliveira, Júlio César de

; Medeiros, Paulo de Tarso Jorge

; Valdigem, Bruno Pereira

; Teno, Luiz Antônio Castilho

; Silva, Rodrigo Tavares

; Melo, Celso Salgado de

; Elias Neto, Jorge

; Moraes Júnior, Antonio Vitor

; Pedrosa, Anisio Alexandre Andrade

; Porto, Fernando Mello

; Brito Júnior, Hélio Lima de

; Souza, Thiago Gonçalves Schroder e

; Mateos, José Carlos Pachón

; Moraes, Luis Gustavo Belo de

; Forno, Alexander Romeno Janner Dal

; D’Avila, Andre Luiz Buchele

; Cavaco, Diogo Alberto de Magalhães

; Kuniyoshi, Ricardo Ryoshim

; Pimentel, Mauricio

; Camanho, Luiz Eduardo Montenegro

; Saad, Eduardo Benchimol

; Zimerman, Leandro Ioschpe

; Oliveira, Eduardo Bartholomay

; Scanavacca, Mauricio Ibrahim

; Martinelli Filho, Martino

; Lima, Carlos Eduardo Batista de

; Peixoto, Giselle de Lima

; Darrieux, Francisco Carlos da Costa

; Duarte, Jussara de Oliveira Pinheiro

; Galvão Filho, Silas dos Santos

; Costa, Eduardo Rodrigues Bento

; Mateo, Enrique Indalécio Pachón

; Melo, Sissy Lara De

; Rodrigues, Thiago da Rocha

; Rocha, Eduardo Arrais

; Hachul, Denise Tessariol

; Lorga Filho, Adalberto Menezes

; Nishioka, Silvana Angelina D’Orio

; Gadelha, Eduardo Barreto

; Costa, Roberto

; Andrade, Veridiana Silva de

; Torres, Gustavo Gomes

; Oliveira Neto, Nestor Rodrigues de

; Lucchese, Fernando Antonio

; Murad, Henrique

; Wanderley Neto, José

; Brofman, Paulo Roberto Slud

; Almeida, Rui M. S.

; Leal, João Carlos Ferreira

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 1 elocation e20220892

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

https://doi.org/10.36660/abc.20220892

Healthcare workers exposed to COVID-19 patients present an inflammatory status and Th2/Th17/Th22 immune profile: findings from before vaccine application in Brazil
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AQUINO, RODRIGO CESAR A. DE

; BARROS, BÁRBARA RAFAELA S.

; SILVA, GUILHERME ANTONIO S.

; SOUSA, GEORON F. DE

; SOUZA, EDSON B. DE

; SILVA, DYEGO R.C.

; NASCIMENTO, ARIONE V. DO

; SÁ, IGOR W.A. DE

; LIMA, ELKER L.S. DE

; SILVA, BÁRBARA O.

; LIMA, LUÍSA P.O. DE

; VIEIRA, AMANDA M.

; BARBOSA NETO, ADAUTO G.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; OLIVEIRA, TALITA HELENA ARAÚJO DE

; CARVALHO, BRUNO M.

; MUNIZ, MARIA TEREZA C.

; FREITAS, ANTONIO CARLOS DE

; CAMPELO JÚNIOR, EVÔNIO B.

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220502

Resumo: En | Texto: En | PDF: En

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

Septic shock, hyperferritinemic syndrome, and multiple organ dysfunction without respiratory failure in a patient with disseminated histoplasmosis and advanced HIV disease
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Silva, Jussemara Souza da

; Ernandes, Bruno Correia

; Fernandes, Carol Lee Luna

; Correia, Ademir Silva

; Ponce, Cesar Cilento

; Sztajnbok, Jaques

; Rodrigues, Camila

; Vidal, José Ernesto

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volume 65 elocation e28

Resumo: En | Texto: En | PDF: En

ABSTRACT AIDS-related disseminated histoplasmosis (DH) can cause septic shock and multiorgan dysfunction with mortality rates of up to 80%. A 41-year-old male presented with fever, fatigue, weight loss, disseminated skin lesions, low urine output, and mental confusion. Three weeks before admission, the patient was diagnosed with HIV infection, but antiretroviral therapy (ART) was not initiated. On day 1 of admission, sepsis with multiorgan dysfunction (acute renal failure, metabolic acidosis, hepatic failure, and coagulopathy) was identified. A chest computed tomography showed unspecific findings. Yeasts suggestive of Histoplasma spp. were observed in a routine peripheral blood smear. On day 2, the patient was transferred to the ICU, where his clinical condition progressed with reduced level of consciousness, hyperferritinemia, and refractory septic shock, requiring high doses of vasopressors, corticosteroids, mechanical ventilation, and hemodialysis. Amphotericin B deoxycholate was initiated. On day 3, yeasts suggestive of Histoplasma spp. were observed in the bone marrow. On day 10, ART was initiated. On day 28, samples of peripheral blood and bone marrow cultures revealed Histoplasma spp. The patient stayed in the ICU for 32 days, completing three weeks of intravenous antifungal therapy. After progressive clinical and laboratory improvement, the patient was discharged from the hospital on oral itraconazole, trimethoprim-sulfamethoxazole, and ART. This case highlights the inclusion of DH in the differential diagnosis of patients with advanced HIV disease, septic shock and multiorgan dysfunction but without respiratory failure. In addition, it provides early in-hospital diagnosis and treatment and comprehensive management in the ICU as determining factors for a good outcome.

https://doi.org/10.1590/s1678-9946202365028

Copper Binary and Ternary Alloys as Anticorrosive Coatings for Carbon Steel in a Saline Medium
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Souza, Thais Machado de

; Mesquita, Gustavo Dionisio

; Loureiro, Bruno S. M.

; Dias, Lucas Ferreira da Silva

; Lago, Dalva C. B. do

; Senna, Lilian F. de

Materials Research

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2023, Volume 26 Supl. 1 elocation e20230076

Resumo: En | Texto: En | PDF: En

This work compares the anticorrosive properties of DC electrodeposited CuCoNi, CuCo, and CuNi alloy coatings in a NaCl 0.5 mol L-1 solution. The results showed that j and the bath composition influenced the chemical, morphological, and electrochemical characteristics of the alloy coatings. Among all the studied coatings, the CuCo, CuNi, and CuCoNi ones produced at j = 10 A m-2, j = 60 A m-2, and j = 25 A m-2, respectively, showed the best anticorrosive performances in the saline medium, exhibiting charge transfer resistances (Rtc) higher than 2000 Ω cm2 and an electrical double layer capacitances (Cdl) lower than 8 X10-4 F cm-2. These results agree with the compact morphologies and smaller grain sizes presented by these coatings. Therefore, the anticorrosive properties of the coatings cannot be related only to the less noble metal(s) contents in the alloy but also to their morphologies.

https://doi.org/10.1590/1980-5373-mr-2023-0076

Weed spatial distribution as a function of soil properties in two distinct environments of the Brazilian semi-arid region
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LESSA, BRUNO F. DA TRINDADE

; RODRIGUES, MARCOS S.

; SOUZA, VALÉRIA CRISTINA DE

; SILVA, KÁTIA A.

; MELO, ANTÔNIO CARLOS DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 4 elocation e20190182

Resumo: En | Texto: En | PDF: En

Abstract The present study aimed was to evaluate the spatial variability of weed species by means of phytosociological parameters and their correlations with the physical-chemical soil properties, under semiarid climate conditions. Weed phytosociology and soil characterization were carried out in two areas one newly deforested area covering 8.86 ha, and one experimental agricultural area covering 24.7 ha; both in the semi-arid region of Brazil. Weed and soil were sampled by following georeferenced grids in each area. Biomass and the total number of weed individuals, as well as soil properties, were mapped by the ordinary Kriging method. The predominant herbaceous plants in the newly deforested area were Hexasepalum teres and Digitaria insularis. The weed species that predominated in the agricultural area were Cyperus rotundus L., Euphorbia heterophylla L. and Herissantia Crispa (L.) Brizicky; the latter species outstanding for dry biomass (873.5g). Spatial dependence was observed for the predominant species, except for Digitaria insularis. The spatial distribution of these weeds was conditioned by soil K+ contents in both areas, and by sand content for the experimental agricultural area. Therefore, these two soil attributes resulted key factors for weed infestation in this semi-arid region.

https://doi.org/10.1590/0001-3765202320190182

10.

Landsat data respond to variations in the structure of Caatinga plant communities along a successional gradient
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SILVA, FERNANDA KELLY G. DA

; MARTINS, FERNANDO ROBERTO

; TEIXEIRA, ADUNIAS DOS SANTOS

; MAS, JEAN-FRANÇOIS

; MENEZES, BRUNO S. DE

; PONZONI, FLAVIO JORGE

; ARAÚJO, FRANCISCA S. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 3 elocation e20230022

Resumo: En | Texto: En | PDF: En

Abstract Plant community succession is generally approached with phytosociological methods, but field surveys are time-consuming, expensive, and limited to several of sites. Remote sensing offers an efficient and economical way to analyze vegetation on large extensions and in inaccessible areas. Most studies addressing remote sensing and tree community succession refer to forest physiognomies. We investigated whether structural changes that occur in non-forest physiognomies are identified by multispectral sensor images (OLI-Landsat). Thirteen 0.1-ha plots were set up in Caatinga fragments aging 10-15, 20-25, 30-35, 40-45 and >50 years to calculate the total density of individuals (TD), mean canopy height (H), total basal area (G) and total aboveground biomass (AGB). We performed correlation analyses between these structural descriptors and eight remote sensing variables (reflectance data and spectral indices) obtained from Landsat images at the end of the rainy season and during the dry season. Blue and short-wave infrared reflectances were negatively correlated with mean height, basal area and biomass, regardless of the analyzed scene (coefficients between -0.58 and -0.79). The litter layer (a non-photosynthetic vegetation component) and the soil exposure are important factors influencing the spectral data.

https://doi.org/10.1590/0001-3765202320230022

11.

Microstructural Evolution of a Hot-Stamped Boron Steel Automotive Part and Its Influence on Corrosion Properties and Tempering Behavior HotStamped Hot Stamped
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Lima, Tiago N.

; Callegari, Bruna

; Folle, Luís Fernando

; Santos, Ygor Tadeu B. dos

; Zamorano, Luiz Gustavo

; Silva, Bruno Caetano dos S.

; Coelho, Rodrigo Santiago

Materials Research

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2023, Volume 26 elocation e20220494

Resumo: En | Texto: En | PDF: En

Boron-manganese steel 22MnB5 is extensively used in structural automotive components. Knowledge about its microstructural evolution during hot stamping and resistance spot welding (RSW) is extremely relevant to guarantee compliance with application requirements. Particularly, corrosion properties are critical to the application of uncoated sheet steels. However, microstructural studies are usually simplified to top-hat geometries, which might not be fully representative of the complex thermomechanical cycles locally faced by a real component. Therefore, the present work brings an extensive characterization of a hot-stamped 22MnB5 automotive B-pillar in terms of microstructure, hardness and corrosion resistance, which were correlated with a reverse engineering of the process using numerical simulation. Physical simulations of the subcritical heat affected zone (SCHAZ) of RSW were done to assess the influence of microstructure on martensite tempering. Results showed that the component undergoes a complex strain distribution along its body during hot stamping. Most heavily strained regions presented higher amounts of ferrite, leading to poorer corrosion resistance, since ferrite behaves as an anode. Physical simulations of the SCHAZ showed that the softening degree due to martensite tempering is solely affected by peak temperature, while other microstructural features appear to exert negligible or no influence. Boronmanganese Boron manganese MnB 22MnB components (RSW requirements Particularly steels However tophat top hat geometries Therefore hotstamped stamped Bpillar B pillar simulation (SCHAZ anode temperature

https://doi.org/10.1590/1980-5373-mr-2022-0494

12.

Different stocks of Prochilodus lineatus along the Cinzas River basin: contributions from a free-flowing tributary in the Capivara Reservoir region, Paranapanema River basin (upper Paraná River) freeflowing free flowing region upper
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Ferreira, Dhiego G.

; Galindo, Bruno A.

; Limeira, Daniel M.

; Garla, Alanna

; Marques, Ana J. C.

; Moreira, Amanda A.

; Rodrigues, Maria V. H.

; Clemente, Roberta C.

; Shimpo, Jhennifer Y.

; Martins, Gabriel A.

; Almeida, Fernanda S.

; Apolinário-Silva, Caroline

; Zanatta, Augusto S.

; Sofia, Silvia H.

; Souza-Shibatta, Lenice

Neotropical Ichthyology

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2023, Volume 21 Nº 4 elocation e230080

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Barragens são umas das principais ameaças para peixes potamódromos Neotropicais, causando bloqueios de rotas migratórias e perdas de áreas de reprodução em um número crescente de sistemas de drenagem. Neste cenário, dados atuais defendem que a manutenção de tributários de fluxo livre em bacias hidrográficas fragmentadas seria uma importante estratégia na conservação de peixes migradores, tanto em situações de panmixia quanto em cenários com diferentes estoques coexistindo, tal como já relatado para Prochilodus lineatus. Este estudo analisou locos microssatélites e haplótipos mitocondriais em amostras de P. lineatus obtidas em diferentes anos ao longo da bacia do rio das Cinzas, no reservatório de Capivara (maior reservatório na calha principal do rio Paranapanema, alto rio Paraná) e no rio Tibagi, todos conectados por trechos livres, visando investigar as contribuições da bacia do rio das Cinzas na dinâmica populacional de peixes migradores na região do reservatório de Capivara. Ambos os marcadores detectaram altos níveis de diversidade genética e indicaram diferentes estoques de P. lineatus ao longo da bacia do rio das Cinzas, sugerindo alguma estruturação genética temporal, bem como a mistura destes estoques na amostra do reservatório de Capivara e de outros pontos, corroborando as relações de estoques neste reservatório e seus tributários. Neotropicais drenagem cenário coexistindo P maior Paranapanema Paraná Tibagi livres temporal pontos

Abstract Dams are one of the main threats to Neotropical potamodromous fish, causing blockages of migratory routes and loss of breeding areas in an increasing number of drainage systems. In this scenario, current data argue that the maintenance of free-flowing tributaries along fragmented watersheds would be an important strategy to the migratory fish conservation, both in panmixia situations and in scenarios with different coexisting stocks, as already reported for Prochilodus lineatus. This study analyzed microsatellite loci and mitochondrial haplotypes in samples of P. lineatus obtained in different years along the Cinzas River basin, as well as in the Capivara Reservoir (largest Reservoir in the Paranapanema main channel, upper Paraná River) and in the Tibagi River, all connected by free stretches, aiming to investigate the contributions of the Cinzas River basin to the population dynamics of migratory fish in the Capivara reservoir region. Both markers detected high genetic diversity levels and indicated different genetic stocks of P. lineatus along the Cinzas River basin, suggesting some temporal genetic structuring, as well as the mixture of these stocks in the sample from the Capivara reservoir and from other points, corroborating the relationships among stocks in this reservoir and its tributaries. systems scenario freeflowing flowing conservation P largest channel stretches region structuring points

https://doi.org/10.1590/1982-0224-2023-0080

13.

Development of a Disposable Pipette Extraction Method Using Coffee Silverskin as an Adsorbent for Chromium Determination in Wastewater Samples by Solid Phase Extraction
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Silva, Weida R.

; Costa, Bruno E. S.

; Batista, Alex D.

; Alves, Vanessa N.

; Coelho, Nivia M. M.

Journal of the Brazilian Chemical Society

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Mai 2022, Volume 33 Nº 5 Páginas 498 - 507

Resumo: En | Texto: En | PDF: En

A procedure using disposable pipette tips adapted for solid-phase extraction, known as DPX SPE, was used as an alternative way for the chromium determination in wastewater samples. DPX-SPE consists of a device which allows a dynamic contact between the sample and the solid-phase. A residue obtained from the processing of coffee beans, denominate coffee silverskin, was used as a new adsorbent. Characterization techniques revealed properties of lignocellulosic materials with potential application for chromium adsorption. The parameters including adsorbent mass (25.00 mg), elution solvent (0.1 mol L-1 HCl, 200 μL) and pH (2.0) were optimized. Thus, 1.00 mL of deionized water for conditioning, 4.00 mL of sample, 2 extraction cycles and 1 elution cycle were the employed conditions. Enrichment factor of 12, limit of detection of 6.00 µg L-1 and relative standard deviation (RSD) 1.3% (n = 3) were obtained. The method proved to be fast, cheap, environmentally friendly, and simple, providing good recoveries (104-113%), and it was satisfactorily applied in real samples.

https://doi.org/10.21577/0103-5053.20210171

14.

PERFORMANCE OF AUTONOMOUS IRRIGATION UNIT VIA MOBILE APPLICATION FOR ARUGULA YIELD
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Rolim, Talyson W. R.

; Silva, Alexsandro O. da

; Uchoa Neto, Francisco A.

; Lemos, José G. de A.

; Costa, Bruno R. S.

Engenharia Agrícola

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2022, Volume 42 Nº spe elocation e20210143

Resumo: En | Texto: En | PDF: En

ABSTRACT Due to the increase in water and energy tariffs, in addition to the limited amount of these resources, the automation of irrigation can help farmers to increase the production of agricultural crops. Therefore, the objective of this research was to evaluate different irrigation managements between manual and automatic in the production of arugula in a protected environment, in order to determine the productive potential in the cultivation of vegetables. The experiment was conducted in randomized blocks with four irrigation management strategies, divided into automatic and manual managements: automatic irrigation management via soil (IAS); automatic irrigation management via climate (IAC); manual irrigation management via soil (IMS) and manual irrigation management via climate (IMC). The treatments were applied to the arugula ( Eruca Sativa L. ) crop during two production cycles, and their effect on biophysical aspects of plants and irrigation water productivity was evaluated. For the fresh mass variable, the IAC (17.75 g plant-1), IAS (12.38 g plant-1), and IMC (8.63 g plant-1) treatments, in the 1st cycle, were statistically similar to each other, whereas in the 2nd cycle, only the IAC (16.29 g plant-1) and IAS (19.80 g plant-1) treatments had this statistical similarity. Automatic managements can be recommended based on this research, however, considering the financial difficulties of the small farmer, IMC may be a desirable option in unfavorable economic conditions.

https://doi.org/10.1590/1809-4430-eng.agric.v42nepe20210143/2022

15.

Experimental composite containing silicon dioxide-coated silver nanoparticles for orthodontic bonding: Antimicrobial activity and shear bond strength
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AGUIAR, Rogéria Christina de Oliveira

; NUNES, Larissa Pereira

; BATISTA, Eduardo Silva

; VIANA, Marina Mariante

; RODRIGUES, Marcela Charantola

; BUENO-SILVA, Bruno

; ROSCOE, Marina Guimarães

Dental Press Journal of Orthodontics

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2022, Volume 27 Nº 3 elocation e222116

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: O presente estudo objetivou investigar a atividade antimicrobiana e a resistência de união ao cisalhamento (RU) de braquetes ortodônticos colados em esmalte bovino, utilizando compósitos experimentais com diferentes concentrações de nanopartículas de prata revestidas com dióxido de silício (NPs Ag@SiO2). Material e Métodos: Cinquenta incisivos bovinos foram divididos em cinco grupos, de acordo com o compósito utilizado (n = 10): G1 - Grupo Controle (Resina Transbond XT); G2 - Compósito Experimental sem NPs Ag@SiO2; G3 - Compósito Experimental com 0,5% de NPs Ag@SiO2; G4 - Composto Experimental com 1% de NPs Ag@SiO2; e G5 - Compósito Experimental com 3% de NPs Ag@SiO2. O teste de RU foi realizado em máquina universal de ensaios mecânicos, e o índice de adesivo remanescente (IAR) foi analisado por microscopia óptica. Para a avaliação da atividade antimicrobiana, biofilme de S. mutans foi formado por três dias em discos de hidroxiapatita. Posteriormente, foram avaliadas as unidades formadoras de colônias (UFC) de S. mutans. Para a análise de RU, foi utilizada a Análise de Variância, seguida do teste de Tukey, com nível de significância estatística de 5%. Os dados de UFC foram analisados por meio do teste de Kruskal-Wallis, seguido do teste post-hoc de Dunn. Os resultados de IAR foram analisados descritivamente. Resultados: Não houve diferença estatisticamente significativa nos valores de RU entre os grupos experimentais e o grupo controle (p> 0,05). A incorporação de 3% de NPs Ag@SiO2 reduziu estatisticamente os valores de RU (p< 0,05), em comparação ao grupo com 1%. A adição de 3% de NPs Ag@SiO2 ao compósito reduziu significativamente a formação de biofilme de S. mutans, em relação ao grupo G2 (p< 0,05). Conclusão: Os compósitos com incorporação de 3% de NPs Ag@SiO2 apresentaram valores de RU semelhantes ao grupo controle e demonstraram significativa atividade antimicrobiana.

ABSTRACT Objective: This study aimed to investigate the antimicrobial activity and shear bond strength (SBS) of orthodontic brackets to bovine enamel using experimental composites with different concentrations of silicon dioxide-coated silver nanoparticles (Ag@SiO2 NPs). Methods: Fifty bovine incisors were divided into five groups according to the composite (n = 10): G1 - Control Group (Transbond XT Resin), G2 - Experimental composite without Ag@SiO2 NPs; G3 - Experimental composite with 0.5% of Ag@SiO2 NPs; G4 - Experimental composite with 1% of Ag@SiO2 NPs; G5 - Experimental composite with 3% of Ag@SiO2 NPs. The SBS test was performed using a universal mechanical testing machine, and the adhesive remnant index (ARI) was analyzed by optical microscopy. For the antimicrobial activity evaluation, Streptococcus mutans (S. mutans) biofilm was formed for three days in hydroxyapatite discs. Posteriorly, S. mutans colony forming units (CFU) were evaluated. For SBS analysis, Analysis of Variance was used, followed by the Tukey test, at a 5% statistical significance level. The CFU data were analyzed by Kruskal-Wallis, followed by Dunn as a post-hoc test. The ARI results were analyzed descriptively. Results: There was no statistically significant difference in SBS values between the experimental and control groups (p>0.05). A 3% incorporation of Ag@SiO2 NPs statistically reduced the SBS values (p<0.05) compared to the 1% group. The addition of 3% of Ag@SiO2 NPs to the composites significantly reduced S. mutans biofilm formation, compared to group G2 (p<0.05). Conclusion: Composites incorporating 3% of Ag@SiO2 NPs presented similar SBS values compared to the control group, and showed significant antimicrobial activity.

https://doi.org/10.1590/2177-6709.27.3.e222116.oar

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