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Epidemiology of hydrocephalus in Brazil
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Dutra, Maurício

; da Silva, Stephanya Covas

; Beggiora, Pâmella da S.

; Santos, Marcelo V.

; Machado, Hélio R.

; Lopes, Luiza da S.

Jornal de Pediatria

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Jun 2023, Volume 99 Nº 3 Páginas 228 - 234

Resumo: En | Texto: En | PDF: En

Abstract Objective Describe the epidemiological profile and social-economic burden that hydrocephalus patients represent to the national public health system, using data available at the online database of the Brazilian Health Ministry (DataSUS). Methods This is a populational study based on descriptive statistics of all clinical and surgical appointments included in the DataSUS database. Data included herein were collected between 2015 and 2021 and subdivided into three main groups, related to hydrocephalus incidence and mortality, hospitalizations, and financial costs. Results In the study period, 3993 new cases of congenital hydrocephalus were diagnosed, with 6051 deaths overall. The mortality rate in the country was 1.5/100000 live births and the prevalence was 0.374/100000 inhabitants. The number of hospitalizations resulting from treatment procedures and complications of hydrocephalus was 137,880 and there was a reduction of up to 27.2% during the SARS-CoV-2 pandemics concerning previous years. Total costs for hydrocephalus management in the country amounted to 140,610,585.51 dollars. Conclusions Hydrocephalus has a significant impact on public health budgets and pediatric mortality rates; however, it is probably underestimated, due to the paucity of demographic data and epidemiological studies in Latin America and, specifically, in Brazil. The dataSUS also has several limitations in accessing certain data related to hydrocephalus, making it difficult to have a more assertive understanding of the disease in Brazil. The results of this study provide important guidance for future research projects in clinical and experimental hydrocephalus and also the creation of public policies for better governance and care of hydrocephalus patients.

https://doi.org/10.1016/j.jped.2022.10.008

Challenges of Multidrug-resistant New Delhi Metallo-beta-Lactamase (NDM-1)-producing Enterobacteriaceae in Kidney Transplant Patients
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Foresto, Renato Demarchi

; Menezes, Lucas Marengo

; Nishimura, Laura Tomoko

; Cristelli, Marina Pontello

; Viana, Laila Almeida

; Santos, Daniel Wagner de Castro Lima

; Requião-Moura, Lúcio R.

; Tedesco-Silva, Helio

; Medina-Pestana, Jose

Brazilian Journal of Nephrology

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Mar 2023, Volume 45 Nº 1 Páginas 116 - 120

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background: The emergence of multidrug-resistant NDM-1-producing enterobacteriaceae strains has become a threat to inpatients, especially to immunosuppressed ones, such as kidney transplant recipients. NDM-1 is a carbapenemase that makes gram-negative bacteria resistant to many types of antibiotics. The incidence of carbapenemase-producing enterobacteria infection in solid organ transplant recipients is around 3 to 10%, with a mortality rate of up to 30%. Methods: We present a case series of 4 patients with NDM-1-producing enterobacteria isolated in urine cultures or rectal swabs. We also conducted a cross-sectional study 30 days after patient identification, collecting surveillance cultures (rectal swab) from all inpatients to assess the extent of spread of this resistance mechanism; a total of 101 patients were included. Results: Two patients were adequately treated with negative control cultures. The other two patients were not treated because they were asymptomatic and had subsequent negative urine cultures. No new colonization was identified in the cross-sectional screening, and no new cases of urinary NDM-1 infection were recorded after a 4-year follow-up. Conclusion: Surveillance for infections caused by multidrug-resistant strains in hospitals treating immunosuppressed patients should be continued and prompt action should be taken in cases of outbreaks of multidrug-resistant infections.

Resumo Histórico: O surgimento de cepas multirresistentes de enterobacteriaceae produtoras de NDM-1 tornou-se uma ameaça para pacientes hospitalizados, especialmente para os imunossuprimidos, como os receptores de transplante renal. NDM-1 é uma carbapenemase que torna as bactérias gram-negativas resistentes a muitos tipos de antibióticos. A incidência de infecção por enterobactérias produtoras de carbapenemas em receptores de transplante de órgãos sólidos é de cerca de 3 a 10%, com uma taxa de mortalidade de até 30%. Métodos: Apresentamos uma série de casos de 4 pacientes com enterobactérias produtoras de NDM-1 isoladas em culturas de urina ou esfregaços retais. Também realizamos um estudo transversal 30 dias após a identificação do paciente, coletando culturas de vigilância (esfregaço retal) de todos os pacientes internados para avaliar a extensão de disseminação deste mecanismo de resistência; foram incluídos um total de 101 pacientes. Resultados: Dois pacientes foram tratados adequadamente com culturas de controle negativo. Os outros dois pacientes não foram tratados porque eram assintomáticos e tiveram culturas de urina negativas subsequentes. Não foi identificada nenhuma nova colonização na triagem transversal, e não foram registrados novos casos de infecção urinária por NDM-1 após um acompanhamento de 4 anos. Conclusão: A vigilância de infecções causadas por cepas multirresistentes em hospitais que tratam pacientes imunossuprimidos deve ser continuada e devem ser tomadas medidas imediatas em casos de surtos desses tipos de infecções.

https://doi.org/10.1590/2175-8239-jbn-2021-0033

Migratory pattern of the coronavirus disease 2019 and high fatality rates among kidney transplant recipients: report from the Brazilian Multicenter Cohort Study
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Cristelli, Marina Pontello

; Sandes-Freitas, Tainá Veras de

; Viana, Laila Almeida

; Requião-Moura, Lúcio R.

; Andrade, Luis Gustavo Modelli de

; Tedesco-Silva, Helio

; Medina-Pestana, José

Brazilian Journal of Nephrology

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Set 2022, Volume 44 Nº 3 Páginas 428 - 433

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Introdução: A pandemia da COVID-19 afetou receptores de transplante renal (TR), com taxas de mortalidade mundial em torno de 25%. Considerando as notórias disparidades sócio-demográficas brasileiras, este relatório descreve pela primeira vez principais características e desfechos da doença em receptores de TR, segundo as regiões geográficas. Métodos: Esta análise retrospectiva multicêntrica nacional incluiu dados de receptores de TR com COVID-19 confirmada entre Março/Novembro de 2020. Resultados: Trinta e cinco dos 81 centros (57% da atividade de transplante renal brasileira) relataram 1.680 pacientes com COVID-19. O Nordeste foi o primeiro a atingir o pico no número de infecções. O Sudeste, por sua densidade populacional, contribuiu com maior número de pacientes. Pacientes tinham em média 52 anos, 76% apresentavam hipertensão e 34% diabetes, 75% receptores de doador falecido e o tempo entre diagnóstico e transplante foi de 5,9 anos. Em 53% dos pacientes, os imunossupressores foram ajustados, e o tratamento variou segundo a região. Hospitalização foi necessária para 65% dos pacientes, 35% necessitaram de cuidados intensivos, 25% ventilação mecânica, e 23% terapia renal substitutiva. A mortalidade geral em 90 dias foi 21%, sendo 23% no Sudeste, 16% no Nordeste, e 19% nas regiões Centro-Oeste e Sul. Conclusão: O padrão migratório da pandemia entre os receptores de TR seguiu o da população em geral e os desfechos foram influenciados por características regionais. A COVID-19 em receptores de TR foi associada à alta utilização de recursos de saúde e taxas de mortalidade mais altas do que as relatadas na população em geral.

Abstract Introduction: The unprecedented coronavirus disease 2019 (COVID-19) pandemic has affected kidney transplant (KT) recipients, with worldwide fatality rates around 25%. Considering the well-known Brazilian socio-demographic disparities, this report describes for the first time the main outcomes of COVID-19 in KT recipients according to Brazilian geographic regions. Methods: This multicenter national retrospective analysis included data from KT recipients with confirmed COVID-19 between March and November 2020. Results: Thirty-five of the 81 centers (57% of KT activity in Brazil) reported 1,680 patients with COVID-19. The Northeast was the first to reach the peak in the number of infections. The Southeast, due to its population density, contributed with the largest number of patients. Patients had a median age of 52 years, 76% had hypertension and 34% diabetes, 75% were recipients of a deceased donor, and the time interval between diagnosis and transplantation was 5.9 years. In 53% of patients, immunosuppression was adjusted, and clinical support varied according to geographic region. Hospitalization was required for 65% of the patients, 35% of them needed intensive care, 25% mechanical ventilation, and 23% renal replacement therapy. The 90-day overall fatality was 21%, being 23% in the Southeast, 16% in the Northeast, and 19% in the Central-west and South regions. Conclusion: The migratory pattern of the pandemic among KT recipients followed that of the general population and the outcomes were influenced by regional features. COVID-19 in KT recipients was associated with high utilization of health-care resources and higher fatality rates than those reported in the general population.

https://doi.org/10.1590/2175-8239-jbn-2021-0063

Mineral Nutrients and Plant-Fungal Interaction in Cocoa Trees (Theobroma cacao L.)
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Sousa Filho, Hélio R.

; Jesus, Raildo M. de

; Bezerra, Marcos A.

; Silva, Vinnícius H. C. da

; Silva Jr., André L. S. da

; Alves, Juscelia P. S.

; Santana, Gregório M.

; Souza Jr., José O. de

Journal of the Brazilian Chemical Society

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Fev 2021, Volume 32 Nº 2 Páginas 337 - 346

Resumo: En | Texto: En | PDF: En

An infection of the fungus Moniliophthora perniciosa in cocoa trees reduces productivity of the plant. In this study, the concentrations of mineral nutrients in healthy leaves and in leaves of vegetative broom of the cocoa plant were determined, and the relationship between the disease and the nutritional composition of the leaves was assessed. The samples were analyzed using the wet digestion method and the concentrations were determined by inductively coupled plasma optical emission spectrometry and the Kjeldahl method. Results indicate that leaves of vegetative broom have lower concentrations of Ca, Mg, S, Fe, Mn, and Ni than healthy leaves. Concentrations of P and Cu were lower in healthy leaves and higher in leaves of vegetative broom. In contrast, concentrations of K, N, and Zn were the same for both types of leaves. In short, plant-fungal interaction was reflected in the nutritional composition of the leaves, demonstrated by the differences in nutrient concentrations between healthy and infected leaf tissue.

https://doi.org/10.21577/0103-5053.20200184 351 downloads

Refractory Ceramics of Clay and Alumina Waste
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Silva, Valmir José da

; Taveira, Salvador K. A.

; Silva, Karina R.

; Neves, Gelmires A.

; Lira, Hélio L.

; Santana, Lisiane N. L.

Materials Research

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2021, Volume 24 Nº 2 elocation e20200485

Resumo: En | Texto: En | PDF: En

Abstract Refractory ceramics were produced from clays and alumina waste. Specimens were shaped by uniaxial pressing, subjected to thermal analysis by dilatometry and heat-treated in a conventional furnace at 1300 and 1400ºC, applying a heating rate of 5ºC/min and a dwell time of 2 and 3 hours at the maximum temperature. The samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and physicomechanical properties. The dilatometric analysis before heat treatment indicated that the formulation containing the largest amount of fluxing oxides presented the highest linear shrinkage. The XRD analysis revealed that mullite was the major phase and needle shaped crystals typical of mullite obtained from clay minerals were observed by SEM. The increase in firing temperature and dwell time at the maximum temperature improved the physicomechanical properties of the specimens. The thermal expansion coefficient (TEC) in the range of 25 to 1000ºC varied from 6.2 to 6.9 x 10-6°C-1.

https://doi.org/10.1590/1980-5373-mr-2020-0485 616 downloads

Transition period produces changes in blood and body composition in mares
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Silva, Micheline O.

; Manso Filho, Hélio C.

; Ribeiro, Bruna Maria

; Ermita, Pedro A.N.

; Monteiro, Lorena C.

; Costa, Caio M.

; Alves, Samuel R.

; Ribeiro Filho, José D.

Pesquisa Veterinária Brasileira

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Out 2019, Volume 39 Nº 10 Páginas 843 - 848

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Avaliações clínicas e metabólicas são necessárias para o monitoramento de éguas gestantes e lactantes, pois refletem a saúde do animal. A condição corporal da égua pode ser um indicador da eficiência reprodutiva. O estudo objetivou determinar as possíveis variações na condição corporal e metabólica em éguas Mangalarga Marchador durante o período de transição. Foram utilizadas 48 éguas distribuídas em dois grupos: Grupo em Manutenção (GM) composto por éguas não gestantes e não lactantes; Grupo em Transição (GT) formado por éguas gestantes e que após o parto tornaram-se lactantes. As análises foram realizadas nos tempos T-60, T-30 e T-15 antes do parto, primeiras seis horas (T0) após o parto e T15, T30 e T60 dias após o parto. O GM foi avaliado apenas uma vez (T-60). O peso corporal e a massa livre de gordura diferiram (P<0,05) entre os grupos. O percentual de gordura foi menor em GM. As éguas apresentaram maior porcentagem de gordura no GT no T-60 e no T-30. Houve diferença (P<0,05) na quantidade de colesterol entre GM e GT (T0, T15 e T30). Triglicérides foram diferentes entre os grupos. GT apresentou maiores concentrações de ácidos graxos não esterificados (P<0,05). Houve maior quantidade de glicose no GT (parto e lactação) quando comparada às éguas no GM. Mudanças na condição corporal e nos constituintes metabólicos ocorreram nos animais resultantes de adaptações fisiológicas do período de transição. Os componentes energéticos são os mais afetados neste período, existindo intensa mobilização de gordura para suprir demandas corporais.

ABSTRACT: Clinical and metabolic evaluation is necessary for the monitoring of pregnant and lactating mares, as they reflect the health of the animal. The body condition of the mare is an indicator of reproductive efficiency. The study aimed to determine the possible variations in body and metabolic condition in Mangalarga Marchador mares during the transition period. Forty-eight mares distributed in two groups were used: Maintenance Group (MG), composed of non-pregnant and non-lactating mares, and Transition Group (TG), formed by pregnant mares and who after delivery became lactating. Analyzes were performed in the times T-60, T-30 and T-15 before delivery, first six hours (T0) after delivery and T15, T30 and T60 days after delivery. MG was evaluated only at one time (T-60). Body weight and fat-free mass differed (P<0.05) between the groups. The percentage of fat was lower in MG. Mares had a higher fat percentage in TG at T-60 and T-30 times. There was a difference (P<0.05) in the amount of cholesterol between MG and TG (T0, T15 and T30). Triglycerides were different between the groups. TG showed higher concentrations of non-esterified fatty acids (P<0.05). There was a higher amount of glucose in TG (delivery and lactation) when compared to mares in MG. Changes in body condition and metabolic constituents occurred in the animals resulting from physiological adaptations of the transition period. The energetic components are the most affected from the transition period, with intense fat mobilization to supply the body demands.

https://doi.org/10.1590/1678-5150-pvb-6337 651 downloads

Yield and quality of sunflower oil in Ultisol and Oxisol under water regimes
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Loose, Luis H.

; Heldwein, Arno B.

; Silva, Jocélia R. da

; Bortoluzzi, Mateus P.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Jul 2019, Volume 23 Nº 7 Páginas 532 - 537

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Eventos naturais extremos que influenciam a disponibilidade hídrica no solo são os principais fatores limitantes da produtividade e da qualidade do óleo das culturas oleaginosas. O objetivo do presente trabalho foi avaliar o efeito do déficit e do excesso hídrico sobre a produtividade, o teor de óleo e o perfil de ácidos graxos do girassol cultivado na Safra e na Safrinha em dois solos do Rio Grande do Sul. O experimento foi conduzido em Santa Maria, RS (Argissolo) e Panambi, RS (Latossolo), e compreende três condições hídricas (déficit hídrico, excesso hídrico e controle) no delineamento de blocos ao acaso. O híbrido Helio 250 foi semeado no início de setembro (Safra) e início de janeiro (Safrinha) em ambos os solos. A produtividade, os componentes de rendimento, o teor de óleo, o rendimento de óleo e o perfil de ácidos graxos foram as variáveis analisadas. A produtividade e os componentes de rendimento foram afetados pela condição hídrica, pela época de semeadura e pela classe de solo. O fator classe de solo não teve efeito significativo sobre o teor e o rendimento de óleo ou qualidade. O déficit hídrico foi mais prejudicial para o rendimento e a qualidade do óleo do que o excesso hídrico. O déficit hídrico foi responsável pelo aumento do ácido oleico e redução do ácido linoleico.

ABSTRACT Extreme natural events influencing soil water availability are major factors limiting yield and oil quality in oilseed crops. The aim of the present study was to assess the effect of water deficit and surplus water on yield, oil content and on the fatty acid profile of sunflower plants sown in main and second crop seasons in two soil classes in Rio Grande do Sul State. The experiment was carried out in Santa Maria, RS (Ultisol) and Panambi, RS state, Brazil (Oxisol), Brazil, and comprised three water conditions (water deficit, water surplus and control) in a randomized block design. The hybrid Helio 250 was sown in early September (main crop season) and in early January (second crop season) in both soil classes. Yield, yield components, oil content, oil yield and fatty acid profile were herein analyzed. Yield and yield components were affected by water conditions, sowing dates and soil class. The soil class had no significant effect on oil content, yield or quality. Water deficit was more harmful to oil yield and quality than water surplus. Water deficit was responsible for increased oleic fatty acid contents and for linoleic content reduction.

https://doi.org/10.1590/1807-1929/agriambi.v23n7p532-537 643 downloads

Zinc Ferrite Nanoparticles via Coprecipitation Modified Method: Glycerol as Structure Directing and Stabilizing Agent
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Lima, Edilan S.

; Costa, Luelc S.

; Sampaio, Gabrielle R. L. M.

; Oliveira, Edipo S.

; Silva, Erandir B.

; Nascimento, Hélio O.

; Nascimento, Ronaldo F.

; Moura, Karine O.

; Bastos-Neto, Moisés

; Loiola, Adonay R.

; Sasaki, José M.

Journal of the Brazilian Chemical Society

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Abr 2019, Volume 30 Nº 4 Páginas 882 - 891

Resumo: En | Texto: En | PDF: En

Coprecipitation is one of the most practical methods used to synthesize ferrite nanoparticles. Fine characteristics of these materials can be improved by means of a series of modifications in the synthesis method. In this paper we present a study of influence of glycerol as structure directing and stabilizing agent in the synthesis of zinc ferrite nanoparticle, exploring its chelating capacity and oxidation. The studied materials include two series of zinc ferrite samples and its precursors obtained with or without glycerol throughout different stages during the synthesis process. The structural and morphological characteristics were evaluated by means of different techniques such as X-ray diffraction, Fourier transformed infrared spectroscopy, scanning and transmission electron microscopy and thermal analysis. The oxidation of glycerol was determined by high-performance liquid chromatograph. The obtained results demonstrate that zinc ferrite crystals synthesized in the presence of glycerol are initially bigger than those synthesized in absence of it, but according to the oxidation process they tend to decrease giving rise to intermediate phases. Interestingly, these samples grow back during more advanced stages and become structurally better organized, compared to the series of samples produced in absence of glycerol. These results indicate that glycerol is capable to modify the synthesis route of zinc ferrite nanoparticles via coprecipitation, acting directly over the size and morphology of the crystals.

https://doi.org/10.21577/0103-5053.20180225 1974 downloads

Development of an indirect ELISA assay to evaluation of the adaptive immune response of pacu (Piaractus mesopotamicus)
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FARIAS, THAIS H.V.

; SILVA, KETHERSON R.

; MARIGUELA, VIVIANE C.

; MONTASSIER, HÉLIO J.

; PILARSKI, FABIANA

Anais da Academia Brasileira de Ciências

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Dez 2018, Volume 90 Nº 4 Páginas 3327 - 3335

Resumo: En | Texto: En | PDF: En

ABSTRACT The pacu is one of the most important species for Brazilian fish farming and is considered emerging in the global aquaculture. Despite its importance, no effective tool for evaluation of the adaptive immune response of this species has been developed. Therefore, this study aimed the development and standardization of indirect ELISA for the measurement of pacu antigen-specific antibodies using polyclonal rabbit anti-pacu IgM used as detector antibody. For this purpose was isolated and purificated of pacu IgM using mannose-binding protein affinity chromatography and produced specific polyclonal antibodies against heavy and light chains pacu IgM, that showed a molecular weight of 72 kDa and 26 kDa, respectively. Polyclonal antibodies obtained demonstrated specificity with heavy and light Ig chains of pacu serum in western blotting. These polyclonal antibodies allowed the development of an indirect ELISA assay of high sensitivity and specificity for the detection and quantification of pacu IgM antibodies immunized with bovine IgG. In conclusion, this approach has great potential to improve the monitoring of vaccine-induced immune responses and help develop immunodiagnostic and epidemiological studies of infectious diseases in pacu systems.

https://doi.org/10.1590/0001-3765201820170438 1313 downloads

10.

The influence of clinical, environmental, and socioeconomic factors on five-year patient survival after kidney transplantation
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Ruppel, Priscila

; Felipe, Claudia R.

; Medina-Pestana, Jose O.

; Hiramoto, Liliane Lumi

; Viana, Laila

; Ferreira, Alexandra

; Aguiar, Wilson

; Ivani, Mayara

; Bessa, Adrieli

; Cristelli, Marina

; Gaspar, Melissa

; Tedesco-Silva Jr, Helio

Brazilian Journal of Nephrology

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Jun 2018, Volume 40 Nº 2 Páginas 151 - 161

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: O risco de óbito após transplante renal está associado à idade do receptor, presença de comorbidades, condição socioeconômica, às características ambientais locais e ao acesso a serviços de atenção à saúde. Objetivo: Investigar as causas e fatores de risco associados ao óbito nos primeiros cinco anos após o transplante renal. Métodos: Este é um estudo unicêntrico retrospectivo com pareamento dos grupos caso e controle. Resultados: Em uma coorte consecutiva de 1.873 receptores de transplante renal atendidos de 1/1/2007 a 31/12/2009 foram registrados 162 óbitos (grupo caso), correspondendo a uma taxa de sobrevida após cinco anos de 91,4%. Dos óbitos registrados, 25% ocorreram nos primeiros três meses após o transplante. A causa de óbito mais prevalente foi infecção (53%), seguida de doença cardiovascular (24%). Os fatores de risco associados a mortalidade foram histórico de diabetes, tipo e tempo em diálise, desemprego, função tardia do enxerto, número de consultas, número de hospitalizações e tempo de internação hospitalar. Após análise multivariada, apenas o tempo em diálise, o número de consultas e dias de internação permaneceram associados a mortalidade. Os pacientes que foram a óbito tiveram um número não significativamente maior de tratamentos de episódios de rejeição aguda (38% vs. 29%; p = 0,078), maior número médio de eventos adversos por paciente (5,1 ± 3,8 vs. 3,8 ± 2,9; p = 0,194) e TFGe média mais baixa aos três meses (50,8 ± 25,1 vs. 56,7 ± 20,7; p = 0,137) e 48 meses (45,9 ± 23,8 vs. 58,5 ± 20,2; p = 0,368). Conclusão: A presente análise confirmou que nessa população, a infecção foi a principal causa de mortalidade nos primeiros cinco anos após transplante renal. Vários fatores de risco demográficos e socioeconômicos foram associados a mortalidade, a maioria não prontamente modificável.

ABSTRACT Introduction: The risk of death after kidney transplant is associated with the age of the recipient, presence of comorbidities, socioeconomic status, local environmental characteristics and access to health care. Objective: To investigate the causes and risk factors associated with death during the first 5 years after kidney transplantation. Methods: This was a single-center, retrospective, matched case-control study. Results: Using a consecutive cohort of 1,873 kidney transplant recipients from January 1st 2007 to December 31st 2009, there were 162 deaths (case group), corresponding to 5-year patient survival of 91.4%. Of these deaths, 25% occurred during the first 3 months after transplant. The most prevalent cause of death was infectious (53%) followed by cardiovascular (24%). Risk factors associated with death were history of diabetes, dialysis type and time, unemployment, delayed graft function, number of visits to center, number of hospitalizations, and duration of hospital stay. After multivariate analysis, only time on dialysis, number of visits to center, and days in hospital were still associated with death. Patients who died had a non-significant higher number of treated acute rejection episodes (38% vs. 29%, p = 0.078), higher mean number of adverse events per patient (5.1 ± 3.8 vs. 3.8 ± 2.9, p = 0.194), and lower mean eGFR at 3 months (50.8 ± 25.1 vs. 56.7 ± 20.7, p = 0.137) and 48 months (45.9 ± 23.8 vs. 58.5 ± 20.2, p = 0.368). Conclusion: This analysis confirmed that in this population, infection is the leading cause of mortality over the first 5 years after kidney transplantation. Several demographic and socioeconomic risk factors were associated with death, most of which are not readily modifiable.

https://doi.org/10.1590/2175-8239-jbn-3865 1659 downloads

11.

Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
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Vidal, José E.

; Toniolo, Carolina

; Paulino, Adriana

; Colombo, Arnaldo L.

; Martins, Marilena dos Anjos

; Meira, Cristina da Silva

; Azevedo, Renata Guise Soares

; Pereira-Chioccola, Vera Lucia

; Gomes, Hélio Rodrigues

; Lazera, Marcia dos Santos

; Oliveira, Augusto C. Penalva de

; Boulware, David R.

Revista do Instituto de Medicina Tropical de São Paulo

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2018, Volume 60 elocation e1

Resumo: En | Texto: En | PDF: En

ABSTRACT Cryptococcal meningitis is the most common cause of opportunistic meningitis in HIV-infected patients in Brazil and causes unacceptable high mortality rates. In this study, HIV-infected patients with a first episode of culture-proven cryptococcal meningitis in cerebrospinal fluid (CSF) were prospectively included in order to evaluate sensitivity of cryptococcal antigen (CrAg) lateral flow assay (LFA) in serum, CSF, whole blood (fingerstick), and fresh urine. In addition, HIV-infected patients with other neurological confirmed diseases were included in order to evaluate the specificity of CrAg LFA in serum. Twenty patients with cryptococcal meningitis were included and in 19 of them, CrAg LFA in CSF, serum, and whole blood were positive (95% sensitivity). In 18 patients, India ink test was positive in CSF (90% sensitivity), and in 16 cases, CrAg LFA was positive in urine (80% sensitivity). Thirty-six HIV-infected patients with other neurological diseases had negative results of CrAg LFA in serum (100% specificity). In conclusion, CrAg LFA in serum, CSF, and whole blood showed high sensitivity and specificity. Whole blood CrAg LFA seems to be a good and reliable strategy to improve AIDS-related cryptococcal meningitis diagnosis in Brazil.

https://doi.org/10.1590/s1678-9946201860001 2016 downloads

12.

Diagnosis and Management of Classica Homocystinuria in Brazil: A Summary of 72 Late-Diagnosed Patients
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Poloni, Soraia

; Hoss, Giovana W.

; Sperb-Ludwig, Fernanda

; Borsatto, Taciane

; Doriqui, Maria Juliana R.

; Leão, Emília K.E.A

; Boa-Sorte, Ney

; Lourenço, Charles M.

; Kim, Chong A.

; Souza, Carolina F. M. de

; Rocha, Helio

; Ribeiro, Marcia

; Steiner, Carlos E.

; Moreno, Carolina A.

; Bernardi, Pricila

; Valadares, Eugenia

; Artigalas, Osvaldo

; Carvalho, Gerson

; Wanderley, Hector Y. C.

; D’Almeida, Vânia

; Santana-da-Silva, Luiz C.

; Blom, Henk J.

; Schwartz, Ida V. D.

Journal of Inborn Errors of Metabolism and Screening

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2018, Volume 6 elocation e180007

Resumo: En | Texto: En | PDF: En

Abstract This study described a broad clinical characterization of classical homocystinuria (HCU) in Brazil. This was a cross-sectional, observational study including clinical and biochemical data from 72 patients (60 families) from Brazil (South, n = 13; Southeast, n = 37; Northeast, n = 8; North, n = 1; and Midwest, n = 1). Parental consanguinity was reported in 42% of families. Ocular manifestations were the earliest detected symptom (53% of cases), the main reason for diagnostic suspicion (63% of cases), and the most prevalent manifestation at diagnosis (67% of cases). Pyridoxine responsiveness was observed in 14% of patients. Only 22% of nonresponsive patients on treatment had total homocysteine levels <100 mmol/L. Most commonly used treatment strategies were pyridoxine (93% of patients), folic acid (90%), betaine (74%), vitamin B12 (27%), and low-methionine diet + metabolic formula (17%). Most patients diagnosed with HCU in Brazil are late diagnosed, express a severe phenotype, and poor metabolic control. Milder forms of HCU are likely underrepresented due to underdiagnosis.

https://doi.org/10.1177/2326409818788900 1352 downloads

13.

Presentation: Challenges from Peripheries
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Vieira da Cunha, Neiva

; Freire, Jussara

; Silva, Hélio R. S.

Vibrant: Virtual Brazilian Anthropology

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2017, Volume 14 Nº 3 elocation e143072

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/1809-43412017v14n3p072 271 downloads

14.

New Epimeric Spirostanol and Furostanol-Type Steroidal Saponins from Cestrum laevigatum L.
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Ribeiro, Paulo R. V.

; Braz-Filho, Raimundo

; Araújo, Ana J.

; Costa-Lotufo, Letícia V.

; Souza, Luciana G. S.

; Nobre Junior, Hélio V.

; Silva, Cecília R. da

; Andrade Neto, João B. de

; Silveira, Edilberto R.

; Lima, Mary Anne S.

Journal of the Brazilian Chemical Society

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Dez 2016, Volume 27 Nº 12 Páginas 2170 - 2180

Resumo: En | Texto: En | PDF: En

Four new epimeric spirostanol and furostanol-type steroidal saponins, (25R,S)-5α-spirostan-2α,3β-diol 3-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl-(1→4)-β-D-galactopyranoside, (25R,S)-5α-spirostan-2α,3β-diol 3-O-β-D-glucopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1→4)-β-D-galactopyranoside, 26-O-β-D-glucopyranosyl-(25R,S)-5α-furost-20-ene,2α,3β-diol 3-O-β-D-galactopyranoside and 26-O-β-D-glucopyranosyl-(25R,S)-5α-furost-20-ene,2α,3β-diol 3-O-β-D-galactopyranosyl-(1→4)-β-D-galactopyranoside, in addition to the known (25R,S)-5α-spirostan-2α,3β-diol 3-O-β-D-galactopyranoside, were isolated from Cestrum laevigatum. Compounds were submitted to cytotoxic activity assays using colorectal adenocarcinoma (HCT-116), human promyelocytic leukemia (HL-60), ovarian carcinoma (OVCAR-8), glioma (SF-295) human cancer cell lines, and the antimicrobial activity was evaluated against Candida parapsilosis, C. albicans, C. krusei, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis.

https://doi.org/10.5935/0103-5053.20160109 921 downloads

15.

Geotechnology application for data acquisition for agricultural and environmental management of the municipality of Vitória Brazil-SP
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ZANARDO, FÁBIO H.

; RODRIGUES, RICARDO A. F.

; SILVA, HELIO R.

; MARQUES, ARTUR P.

; FARIA, GLAUCIA A.

Engenharia Agrícola

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Ago 2016, Volume 36 Nº 4 Páginas 684 - 695

Resumo: En | Texto: En | PDF: En

ABSTRACT The present study aimed at associating geoprocessing procedures for analysis of land use and native vegetation for municipal management, using an easy access and low cost methodology to the public administration of the municipality of Vitória Brazil/SP. Initially, the land use and the land occupation were evaluated, being delimited the municipal hydrographical micro basin and the permanent preservation areas (PPAs). For this purpose, multispectral images of high spatial resolution were used, available by the government partnerships between the City Hall, the state and federal authorities. From the obtained results it was possible to efficiently determine that only 3% of the original vegetation is preserved; the livestock is the main municipal occupation, covering more than 41% of the area; citrus and sugar-cane are also very expressive, occupying, respectively 13% and 7% of the area of the municipality of Vitoria; and around 70% of the municipal PPAs need hydrophilic species for its recovering. These indicators configure a data bank for consulting and supporting the public admiration, as well as a source for the administrative decision making, aiming at guiding the resources for the development of the municipal rural sector, in a more dynamic, efficient and economically viable way.

https://doi.org/10.1590/1809-4430-Eng.Agric.v36n4p684-695/2016 1079 downloads

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