Resumo Introdução: O coronavírus pertence a uma família de vírus RNA que pode causar infecção respiratória com possibilidade de manifestações gastrintestinais em torno de 5% a 50% dos casos. Objetivo: Relatar caso operado com diagnóstico de COVID-19 e evolução com abdome agudo perfurativo e pneumotórax. Relato do caso: Paciente do sexo feminino de 80 anos com sintomas respiratórios com tosse seca e febre e dor abdominal difusa com sinais de peritonite. Apresentava leucocitose, disfunção renal e aumento de D-dímero com PCR positivo para COVID. Tomografia computadorizada de tórax e abdome demonstrando pneumotórax à direita e extenso pneumoperitônio. Conclusão: A apresentação do COVID-19 com sérias complicações pulmonar e abdominal requer tratamentos especializados e em regime de emergência, entretanto com altas taxas de mortalidade.

Abstract Introduction: The Coronavirus belongs to a family of RNA viruses that can cause respiratory infection, with the possibility of gastrointestinal manifestations in approximately 5-50% of the cases. Objective: To report a surgical case with a diagnosis of COVID-19 that developed acute perforated abdomen and pneumothorax. Case report: This was an 80-year-old female patient with respiratory symptoms, with dry cough and fever and diffuse abdominal pain with signs of peritonitis. She had leukocytosis, kidney dysfunction and an increase in D-dimer with positive PCR for COVID. Computed tomography of the chest and abdomen showed pneumothorax on the right and extensive pneumoperitoneum. Conclusion: The presentation of COVID-19 with severe pulmonary and abdominal complications requires specialized and emergency treatments, but it has high mortality rates.

ABSTRACT BKV and JCV belong to the Polyomaviridae family and are opportunistic agents associated with complications in immunocompromised individuals. Although a single screening assay for both viruses would be convenient, the diversity of BKV and JCV serotypes and genotypes is a methodological challenge. In this paper, we developed a PCR method able to detect and segregate BKV and JCV, despite these genetic discrepancies. A duplex semi-nested PCR (duplex snPCR) was designed to target a conserved region (639nt-1516nt) within the VP2 gene. In the first PCR, a primer set common to all BKV and JCV serotypes/ genotypes was used, followed by a semi-nested PCR with internal primers for BKV and JCV segregation. The limit of detection of the duplex snPCR was as low as 10 copies of BKV or JCV plasmids/μL. Specific products were observed when JCV and BKV plasmids were mixed in the same reaction. In field sample testing, the duplex snPCR detected and distinguished both viruses in different biological samples. Results were confirmed by Sanger's sequencing. The geographical complexity of BKV and JCV serotypes and genotypes imposes limits to a simple and universal method that could detect each virus. However, we describe here a sensitive and reliable PCR technique for BKV and JCV diagnosis that overcomes these limitations and could be universally applied.

Resumo: O presente estudo teve como objetivo identificar o adequado estádio de maturação para colheita de frutos bem como a temperatura ideal para germinação de sementes de pinhão manso. Os frutos foram colhidos de forma aleatória para compor uma amostra composta. Após a colheita, estes frutos foram classificados de acordo com a coloração, em três estádios de maturação que constituíram os seguintes tratamentos: Estádio I: frutos amarelos, com sementes pretas brilhantes; Estádio II: frutos amarelos, com mais de 50% em tom marrom escuro, com sementes pretas brilhantes; Estádio III: frutos pretos e secos, com sementes pretas foscas. Posteriormente, foram realizadas avaliações do grau de umidade, germinação, condutividade elétrica e absorção de água nas diferentes temperaturas (20, 25, 30, 35 e 40 °C). O delineamento experimental foi o esquema fatorial 3x5 com quatro repetições. O máximo de germinação ocorre na temperatura de 35º C, recomenda-se colher as sementes de pinhão manso no estádio II de maturação.

Abstract: The present study had as objective to identify the adequate maturation stage for fruit harvest as well as the ideal temperature for germination of Jatropha curcas seeds. The fruit were collected to form a composite sample, and then classified by color in three maturity stages as follows: Stage I - yellow fruit with shiny black seeds; Stage II - yellow fruit, with more than 50% brownish black, and shiny black seeds; Stage III - dry black fruit with matt black seeds. Subsequently, evaluations of the degree of moisture, germination, electrical conductivity and water absorption at different temperatures (20, 25, 30, 35 and 40 °C). The study followed a 3x5 factorial experimental design with four replications. The maximum germination occurs at a temperature of 35 ºC, it is recommended to harvest the seeds of Jatropha curcas at maturity stage II.

Resumo Objetivo: Estimar o kerma no ar na superfície de entrada (Ka,e) e o kerma no ar na região de órgãos radiossensíveis em radiografias pediátricas dos seios paranasais. Materiais e Métodos: Foram coletados os dados dos pacientes e parâmetros de irradiação em exames de seios paranasais de crianças com 0 a 15 anos, em dois hospitais infantis na cidade do Recife, PE. O Ka,e foi estimado utilizando os rendimentos dos tubos de raios X e os parâmetros selecionados. Os valores de kerma no ar nas regiões dos olhos e tireoide foram estimados utilizando dosímetros termoluminescentes. Resultados: Os valores de Ka,e variaram de 0,065 a 1,446 mGy para radiografias cavum, 0,104 a 7,298 mGy para Caldwell e 0,113 a 7,824 mGy para Waters. Os valores de kerma no ar na região dos olhos variaram de 0,001 a 0,968 mGy para cavum e 0,011 a 0,422 mGy para Caldwell e Waters. Na região tireóidea, os valores de kerma no ar variaram de 0,005 a 0,932 mGy para cavum e 0,002 a 0,972 mGy para Caldwell e Waters. Conclusão: Os parâmetros de irradiação utilizados pelas instituições foram mais elevados do que os recomendados em protocolos britânicos. Ações de otimização são recomendadas de modo a contribuir para a redução da radiação nos pacientes e, portanto, os riscos.

Abstract Objective: To estimate the entrance surface air kerma (Ka,e) and air kerma in the region of radiosensitive organs in radiographs of pediatric paranasal sinuses. Materials and Methods: Patient data and irradiation parameters were collected in examinations of the paranasal sinuses in children from 0 to 15 years of age at two children's hospitals in the city of Recife, PE, Brazil. We estimated the Ka,e using the X-ray tube outputs and selected parameters. To estimate the air kerma values in the regions of the eyes and thyroid, we used thermoluminescent dosimeters. Results: The Ka,e values ranged from 0.065 to 1.446 mGy in cavum radiographs, from 0.104 to 7.298 mGy in Caldwell views, and from 0.113 to 7.824 mGy in Waters views. Air kerma values in the region of the eyes ranged from 0.001 to 0.968 mGy in cavum radiographs and from 0.011 to 0.422 mGy in Caldwell and Waters views . In the thyroid region, air kerma values ranged from 0.005 to 0.932 mGy in cavum radiographs and from 0.002 to 0.972 mGy in Caldwell and Waters views. Conclusion: The radiation levels used at the institutions under study were higher than those recommended in international protocols. We recommend that interventions be initiated in order to reduce patient exposure to radiation and therefore the risks associated with radiological examination of the paranasal sinuses.

Background: Metabolic syndrome (MS) is suggested to be associated with a low grade inflammation state, but the relationship between inflammation biomarkers and the components of metabolic syndrome in adolescents are still lacking. Objective: To investigate the association between C-reactive protein (CRP) serum concentrations and metabolic syndrome components in adolescents. Methods: A cross-sectional population based study was conducted. Anthropometric, biochemical and clinical data were collected from 524 adolescents (11-15 years old) randomly sampled from school population of Alegre city, Espmto Santo, Brazil. Data were analyzed by STATA version 9.0. Results: Adolescents with higher values for BMI (p = 0.001) and higher body fat percentage (p = 0.003) had higher CRP concentrations than those with lower BMI and body fat percentage. CRP concentrations was directly correlated with BMI (r = 0.17, p = 0.0001), waist circumference (r = 0.15, p = 0.0005), HDL-c (r = 0.13, p = 0.003), fasting insulin (r = 0.12, p = 0.003) and systolic blood pressure (r = 0.11, p with = 0.01). In the multiple linear regression analysis BMI (r = 0.05, p = 0.002), fasting glucose (r = -0.01, p = 0.003) and HDL-c (r = 0.017, p < 0.001) were associated to CRP concentrations after adjusting for the other components of MS. Conclusion: The association found between individual components of MS and CRP concentrations suggests that inflammation might be an early event in the development of metabolic disorders in adolescents.

Antecedentes: El síndrome metabólico (SM) se sugiere que está asociada con un estado de inflamación crónica de bajo grado, pero la relación entre biomarcadores de inflamación crónica de bajo grado, pero la relación entre biomarcadores de inflamación y los componentes del síndrome metabólico en adolescentes son escasos. Objetivo: Investigar la asociación entre las concentraciones séricas de proteína C reactiva (CRP) y los componentes del síndrome metabólico en adolescentes. Metodología: Hemos realizado una población basada en estudio de corte transversal. Los datos antropométricos, bioquímicos y clínicos se obtuvieron de 524 adolescentes (11-15 años de edad) seleccionados al azar de la población escolar de la ciudad Alegre, Espírito Santo, Brasil. Los datos fueron analizados por STATA versión 9.0. Resultados: Los adolescentes, con valores más altos de IMC (p = 0,001) y mayor porcentaje de grasa corporal (p = 0,003) tuvieron mayores concentraciones de PCR que aquellos con menor IMC y porcentaje de grasa corporal. Las concentraciones de PCR se correlacionó directamente con el IMC (r = 0,17, p = 0,0001), la circunferencia de la cintura (r = 0,15, p = 0,0005), HDL-c (r = 0,13, p = 0,003), la insulina en ayunas (r = 0,12, p = 0,009) y la presión arterial sistólica (r = 0,11, p = a 0,01). En el análisis de regresión lineal múltiple IMC (r = 0,05, p = 0,002), la glucosa en ayunas (r = -0,01, p = 0,003) y HDL-c (r = 0,017, p < 0,001) se asociaron a las concentraciones de PCR después de ajustar por los otros componentes de SM. Conclusión: La asociación encontrada entre los componentes individuales de SM y las concentraciones de PCR sugiere que la inflamación podría ser un evento temprano en el desarrollo de trastornos metabólicos en los adolescentes.

O zoneamento do potencial de uso de sistemas de resfriamento evaporativo (SRE) acoplado a um sistema de ventilação em modo túnel (SVT) foi realizado no presente trabalho, com vistas à criação de frangos de corte na região Sudeste do Brasil. Quatro configurações do sistema foram testadas: a) SVT; b) SVT associado à SRE do tipo material poroso umedecido com 40% de eficiência (SVTPAD40); c) SVT associado à SRE do tipo material poroso umedecido com 70% de eficiência (SVT PAD70) e d) SVT PAD70 associado a sistema de nebulização (SVT PAD70+NEB). O ambiente térmico foi caracterizado por meio do índice de temperatura e umidade, enquanto o desempenho das aves foi determinado pelo consumo de ração diário, ganho de peso diário e conversão alimentar. Dados meteorológicos de 1980 a 2001, disponibilizados pelo Projeto Reanálise-2 do NCEP/NCAR, foram utilizados nesta pesquisa. Verificou-se o efeito benéfico do uso dos SRE, com SVT PAD70+NEB proporcionando as melhores condições de produção, em especial nos meses de setembro e outubro para quase toda a região Sudeste, com destaque para as mesorregiões Norte e Noroeste de Minas Gerais.

In present research the potential use of evaporative cooling systems (ECS) associated to tunnel ventilation system (TVS) for broiler reared in Southeastern Brazil was studied. Four configuration systems were tested: (a) TVS, (b) TVS associated to a pad cooling system with 40% efficiency (TVSPAD40), (c) TVS associated to a pad cooling system with 70% efficiency (TVSPAD70) and (d) TVSPAD70 associated to a misting system (TVSPAD70+MIST). Thermal environment was characterized by temperature-humidity index, and the chicken performance was estimated by daily feed intake, daily weight gain and feed conversion. Climatic data from 1980 to 2001, released through Reanalysis-2 Project from NCEP/NCAR were used in this research. The benefitial effect of the use of ECS with TVSPAD70+MIST for providing better production condition was verified, especially during September and October for almost all the southeastern region, mainly in northern and northwestern part of the Minas Gerais State.