Pesquisa | SciELO

ABSTRACT This study aimed to evaluate the efficiency of the parameters fresh leaf mass and stomatal density in the estimation of ploidy and pre-selection of putative banana autotetraploids. Young plants of the diploid cultivar Ouro (AA), previously subjected to in vitro polyploidization with the antimitotic amiprofos-methyl - APM (0, 10, 20, 30, 40, and 60 µM L-1) and colchicine (2.5 mM L-1) were evaluated for survival, height, number of leaves, pseudostem diameter, leaf disc fresh mass, and stomatal density. Ploidy was determined by flow cytometry in a random sample of 200 plants to find the relationship between the genomic content and the analyzed variables. Spearman’s correlation analysis showed a strong correlation (0.84**) between leaf disc fresh mass and tetraploid plants, as well as an absence of significant correlation between stomatal density and ploidy levels. Thus, the pre-selection of plants was carried out using a reference value of leaf disc fresh mass and resulted in 688 plants pre-selected as putative tetraploids. Among them, 318 were confirmed as tetraploids, 291 as diploids, and 79 as mixoploids after flow cytometry analysis. The pre-selection efficiency was 46.2% of tetraploid plants from the total pre-selected. The results indicate that the use of leaf disc fresh mass is a simple, practical, and promising method to estimate ploidy when a high number of plants is obtained in in vitro banana polyploidization studies, reducing the total number of plants to have confirmed ploidy.

RESUMO Este estudo objetivou avaliar a eficiência dos parâmetros massa fresca foliar e densidade estomática na estimativa da ploidia e pré-seleção de autotetraploides putativos de bananeira. Plantas jovens da cultivar diploide Ouro (AA), previamente submetidas à poliploidização in vitro com os antimitóticos amiprofos-metil - APM (0, 10, 20, 30, 40 e 60 µM L-1) e colchicina (2,5 mM L-1) foram avaliadas quanto à sobrevivência, altura, número de folhas, diâmetro do pseudocaule, massa fresca de disco foliar e densidade estomática. Determinou-se a ploidia, via citometria de fluxo, numa amostra aleatória de 200 plantas, a fim de encontrar relação entre o conteúdo genômico e as variáveis analisadas. Por meio da análise de correlação de Spearman, verificou-se forte correlação (0,84**) entre a massa fresca de disco foliar e as plantas tetraploides, bem como ausência de correlação significativa entre a densidade estomática e os níveis de ploidia. Assim, a pré-seleção das plantas foi feita mediante um valor de referência de massa fresca de disco foliar e resultou em 688 plantas pré-selecionadas como tetraploides putativos. Destas, 318 foram confirmadas como tetraploides, 291 como diploides e 79 mixoploides, após análise de citometria de fluxo. A eficiência de pré-seleção foi de 46,2% de plantas tetraploides do total pré-selecionado. Os resultados indicam que o uso da massa fresca de disco foliar é um método simples, prático e promissor para estimar a ploidia quando é obtido um elevado número de plantas em trabalhos de poliploidização in vitro da bananeira, reduzindo o total de plantas a ter a ploidia confirmada.

https://doi.org/10.1590/1983-21252023v36n302rc

COVID-19 Infection Changes the Functions and Morphology of Erythrocytes: A Multidisciplinary Study
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Sales, Marcos V. S.

; Tanabe, Eloiza L. L.

; Maciel, Thamilla M. S.

; Tavares, Maria C.

; Leal, Juliana G. C.

; Pinto, Larissa S.

; Pires, Keyla S. N.

; Coelho, Jorge A. P. M.

; Silva, Elaine C. O.

; Souza, Samuel T.

; Fonseca, Eduardo J. S.

; Fragoso, Thiago S.

; Aquino, Thiago M.

; Borbely, Alexandre U.

; Rocha, Ueslen

; Santos, Josué C. C.

; Leite, Ana Catarina R.

Journal of the Brazilian Chemical Society

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Ago 2023, Volume 34 Nº 8 Páginas 1185 - 1196

Resumo: En | Texto: En | PDF: En

Hematological problems are associated with Coronavirus disease 2019 (COVID-19). Respiratory impairment is the higher point studied, although without experimental studies related to the oxygen transport performed by erythrocytes. Therefore, we decided to investigate if erythrocytes from COVID-19 patients have their functionality changed. The case-control study included hospitalized patients with a positive real-time polymerase chain reaction (RT-PCR) result admitted to University Hospital. Volunteers (negative RT-PCR results) were recruited as a control group. Thus, we assessed different erythrocytes parameters, oxidative stress markers, and biophysical studies using whole blood and isolated hemoglobin. We found a decrease of 51% in oxygen uptake and reduced antioxidant enzyme activity in COVID-19 patients compared to controls. Raman spectrometry showed structural changes in the hemoglobin and lipids of the erythrocytes from COVID-19 patients; thus, these results were consolidated with an increase in Young’s modulus in erythrocytes followed by morphology changes. Besides, isolated hemoglobin from COVID-19 patients has a distinct interaction profile using a ligand model compared to the control. COVID-19 leads to structural, functional, and morphological damage to human erythrocytes. Our data showed structural and molecular changes and induction of oxidative stress in erythrocytes by COVID-19, a new perspective on the contribution of erythrocytes to a respiratory commitment in COVID-19.

https://doi.org/10.21577/0103-5053.20230031

Morphophysiology and quality of Alibertia edulis seedlings grown under light contrast and organic residue
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Santos, Cleberton C.

; Goelzer, Ademir

; Silva, Orivaldo B. da

; Santos, Fernando H. M. dos

; Silverio, Juliana M.

; Scalon, Silvana de P. Q.

; Vieira, Maria do C.

; Zárate, Néstor A. Heredia

Revista Brasileira de Engenharia Agrícola e Ambiental

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Mai 2023, Volume 27 Nº 5 Páginas 375 - 382

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Organic residue in the substrate and the availability of light influence the production of high-quality seedlings for ex situ cultivation of native forest species. This study aimed to evaluate the effect of doses of chicken manure incorporated in the soil and shading levels on the morphophysiological responses and quality of Alibertia edulis (Rich.) A. Rich seedlings. Five doses of chicken manure (CM) were tested, 0.00, 2.08, 4.16, 6.24, and 8.32 g CM kg-1 soil, under two shade conditions, 0% (full sun) and 50% shading. At 215 days after transplanting, the maximum height obtained for A. edulis was 47.35 cm with 5.64 g CM kg-1 soil under 50% shade. Shaded seedlings had a higher number of leaves. The maximum leaf and root areas were 796.64 and 118.83 cm2 with 5.32 and 4.21 g CM kg-1 soil, respectively, both under 50% shade. Seedlings in 0% shade had lower physiological indices. Shading levels did not affect stomatal limitation, but CM contributed to physiological efficiency. Shoot biomass was higher under 50% shade with higher CM doses. The highest quality indexes occurred with 3.98 and 6.28 g kg-1 CM under 0 and 50% shade, respectively. A. edulis seedlings responded positively to the addition of CM to the soil and presented plasticity under contrasting light conditions. A. edulis seedlings produced under 50% shading with the addition of 6.24 g CM kg-1 soil presented high morphophysiological and seedling quality indicators. The addition of 4.32 g CM kg-1 soil contributed to high seedling quality under full sun conditions.

RESUMO O uso de resíduo orgânico no substrato e a disponibilidade de luz influenciam na obtenção de mudas de elevada qualidade para o cultivo ex situ de espécies florestais nativas. Objetivou-se com este estudo avaliar o efeito de doses de cama de frango incorporadas ao solo e níveis de sombreamento sobre as respostas morfofisiológicas e qualidade de mudas de Alibertia edulis (Rich.) A. Rich. Foram estudadas cinco doses de cama de frango (CF): 0,00; 2,08; 4,16; 6,24 e 8,32 g kg-1 sob duas condições de sombreamento: 0% (pleno sol) e 50% de sombreamento. Aos 215 dias após o transplantio, a máxima altura da A. edulis foi 47,35 cm com 5,64 g CF kg-1 solo sob 50% de sombreamento. Mudas sombreadas tiveram maior número de folhas. A máxima área foliar e radicular foi de 796,64 e 118,83 cm2 com 5,32 g e 4,21 g CF kg-1 solo, respectivamente, ambos sob 50% de sombra. Mudas sob 0% de sombra tiveram menores índices fisiológicos. Os níveis de sombreamento não afetaram a limitação estomática, mas a CF contribuiu na eficiência fisiológica. As biomassas da parte aérea foram maiores sob 50% de sombra com maiores doses de CF. Os maiores índices de qualidade ocorreram com 3,98 e 6,28 g CF kg-1 solo sob 0 e 50% de sombra, respectivamente. Mudas de A. edulis responderam positivamente à adição de CF ao solo e apresentaram plasticidade às condições contrastantes de luz. Mudas de A. edulis produzidas em 50% de sombra com adição de 6,24 g CF kg-1 solo apresentaram maiores indicadores morfofisiológicos e qualidade de mudas. A adição de 4,32 g CF kg-1 solo contribuiu na qualidade de mudas sob pleno sol.

https://doi.org/10.1590/1807-1929/agriambi.v27n5p375-382

Body fat, cardiovascular risk factors and polymorphism in the FTO gene: randomized clinical trial and different physical exercise for adolescents
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Bila, Wendell C.

; Romano, Márcia C.C.

; Santos, Luciana L. dos

; Silva, Valmin R. da

; Capanema, Flavio D.

; Pfrimer, Karina

; Ferriolli, Eduardo

; Alves, Natália M.C.

; Campos, Cezenário G.

; Carlos, Fabiângelo M.

; Santos, Maria E.S.M. dos

; Lamounier, Joel A.

Jornal de Pediatria

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Abr 2023, Volume 99 Nº 2 Páginas 139 - 146

Resumo: En | Texto: En | PDF: En

Abstract Objective: To investigate the effects of different physical exercise programs and polymorphisms of the FTO (fat mass and obesity-associated gene) on body composition and cardiovascular risk factors in adolescents with overweight and obesity. Methods: A randomized, parallel, double-blind clinical trial consisting of the adolescent overweight from the state public network, in a simple representative random sample, who participated in an aerobic exercise or weight training intervention for 10 weeks. Anthropometry, body composition, biochemical markers, sexual maturation, and rs9939609 polymorphism in the FTO gene were assessed. 347 adolescents had their characterization of nutritional status. 72 individuals with overweight and obesity were invited to participate. 39 remained for the start of the program and were randomly allocated to both types of intervention. In the end, 26 subjects participated in the intervention programs, with 12 and 14 in the aerobic and weight training programs, respectively. Results: Heterozygous and homozygous bearers of risk allele A participating in the aerobic program showed improvements in glycemia (p = 0.002) and total cholesterol (p = 0.023) and a reduction in body fat mass (p = 0.041). The weight training program reduced glycemia in patients with the risk allele A (p = 0.027). Cameron’s stage four sexual maturation participants were 2.1 times more likely to improve their body fat (CI = 1.31–3.39). Conclusion: Aerobic exercises produced exclusively a significant decrease in fat mass and total cholesterol in patients with risk allele A. Distinct physical exercise programs may cause diverse changes in risk variables related to the health of adolescents.

https://doi.org/10.1016/j.jped.2022.07.004

Asociación entre los niveles de vitamina B12 y el deterioro cognitivo en personas mayores
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Aguilar-Navarro, Sara G.

; Carbajal-Silva, Juan C.

; Palacios-Hernández, María G. I.

; Gutiérrez-Gutierrez, Lidia A.

; Ávila-Funes, José A.

; Mimenza-Alvarado, Alberto J.

Gaceta médica de México

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Fev 2023, Volume 159 Nº 1 Páginas 32 - 37

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Introducción: Aún es controversial si la deficiencia de vitamina B12 se asocia a alteraciones cognitivas. Objetivo: Conocer la asociación entre los niveles séricos de vitamina B12 y el desempeño cognitivo. Métodos: Se incluyeron 241 personas ≥ 60 años con medición de niveles séricos de vitamina B12. Se realizó evaluación física y cognitiva y se formaron tres grupos: cognición normal (CN), deterioro cognitivo leve (DCL) y demencia. Los niveles de vitamina B12 se clasificaron en suficiencia (> 400 pg/mL), deficiencia subclínica (201-400 pg/mL) y deficiencia absoluta (≤ 200 pg/mL). Se realizó análisis de regresión lineal multivariado para evaluar la asociación entre función cognitiva y niveles de vitamina B12 después de controlar las variables confusoras. Resultados: La media de edad fue 81.4 ± 8.0 años; 68 % fue del sexo femenino; 17.8 y 39.8 % presentaron deficiencia absoluta y subclínica de vitamina B12; 80 individuos (33 %) cumplieron los criterios de DCL y 70 (29 %), de demencia. Después de ajustar por edad, sexo y escolaridad, los sujetos con DCL y demencia tuvieron niveles más bajos de vitamina B12 comparados con aquellos con CN (p = 0.019). Conclusiones: Se observó asociación estadísticamente significativa entre el desempeño cognitivo global y los niveles bajos de vitamina B12.

Abstract Introduction: Whether vitamin B12 deficiency is associated with cognitive impairment remains controversial. Objective: To determine the association between vitamin B12 serum levels and cognitive performance. Methods: Two-hundred and forty-one adults aged ≥ 60 years who had serum vitamin B12 serum levels measurement were included. Physical and cognitive evaluation was carried out, and three groups were formed: normal cognition (NC), mild cognitive impairment (MCI) and dementia. Vitamin B12 levels were classified as sufficiency (> 400 pg/mL), subclinical deficiency (201-400 pg/mL), and absolute deficiency (≤ 200 pg/mL). Multivariate linear regression analysis was used to evaluate the association between cognitive function and vitamin B12 levels after controlling for confounding variables. Results: Mean age was 81.4 ± 8.0 years; 68% were females; 17.8 % and 39.8% had absolute and subclinical vitamin B12 deficiency, respectively; 80 individuals (33%) met the criteria for MCI, and 70 (29%), for dementia. Those with MCI and dementia had lower vitamin B12 levels in comparison with those with NC after adjusting for age, gender and educational level (p = 0.019). Conclusions: A statistically significant association was observed between global cognitive performance and levels of vitamin B12.

https://doi.org/10.24875/gmm.22000187

Evaluation of pediatric diabetes mellitus after SARS-CoV-2 infection: A long-term prospective case series
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Fink, Thais T.

; Canton, Ana P.M.

; Pereira, Maria Fernanda B.

; Bain, Vera

; Matsuo, Olívia

; Astley, Camilla

; Marques, Heloisa H.S.

; Correa-Silva, Simone

; Montenegro, Marilia M.

; Palmeira, Patricia

; Garanito, Marlene P.

; Duarte, Alberto J.S.

; Carneiro-Sampaio, Magda

; Latronico, Ana C.

; Silva, Clovis A.

Clinics

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2023, Volume 78 elocation 100299

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1016/j.clinsp.2023.100299

Emotional, hyperactivity and inattention problems in adolescents with immunocompromising chronic diseases during the COVID-19 pandemic
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Campos, Reinan T.

; Lindoso, Livia

; Sousa, Renan A. de

; Helito, Alberto C.

; Ihara, Bianca P.

; Strabelli, Claudia A.A.

; Paradelas, Levi M.V.

; Carneiro, Beatriz O.L.

; Cardoso, Maria Paula R.

; Souza, Jean Paulo V. de

; Freire, Marianna R. de M.

; Astley, Camilla

; Laurentino, Moisés F.

; Buscatti, Izabel M.

; Kozu, Katia

; Aikawa, Nadia E.

; Sallum, Adriana M.E.

; Ferreira, Juliana CO.

; Simon, Juliana R.

; Viana, Vivianne S.L.

; Queiroz, Ligia B.

; Gualano, Bruno

; Roschel, Hamilton

; Pereira, Rosa Maria R.

; Toma, Ricardo K.

; Watanabe, Andréia

; Grangeiro, Patricia M.

; Casella, Caio B.

; Farhat, Sylvia C.

; Polanczyk, Guilherme V.

; Campos, Lucia Maria A.

; Silva, Clovis A.

Clinics

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2023, Volume 78 elocation 100167

Resumo: En | Texto: En | PDF: En

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics.

https://doi.org/10.1016/j.clinsp.2023.100167

Investigação da atividade eletroquímica de liga Al-Cu-Li após processo de soldagem por fricção e mistura
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Silva, Rejane Maria P. da

; Milagre, Mariana X.

; Araujo, João Victor de S.

; Ramirez, Oscar Mauricio P.

; Machado, Caruline de Souza C.

; Antunes, Renato A.

; Costa, Isolda

Química Nova

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2023, Volume 46 Nº 4 Páginas 307 - 318

Resumo: Pt | Texto: Pt | PDF: Pt

In this work, the local electrochemical activity of the zones coupled by Friction Stir Welding (FSW) of an Al-Cu-Li alloy was studied and the results were correlated to the microstructural characteristics of each zone. Electrochemical studies were carried out in the zones affected by welding using cyclic voltammetry (CV) and scanning electrochemical techniques (namely, SECM - Scanning Electrochemical Microscopy and LEIS - Local electrochemical impedance spectroscopy). The results showed that the welding joint (WJ) is predominantly cathodic relatively to the heat affected zones (HAZ). The HAZ was always anodic and showed the highest electrochemical activities among the tested ones. The high electrochemical activity of the HAZ was associated with the effect of galvanic coupling between the cathodic region (WJ) and the anodic region (HAZ). In addition, the advancing side (AS) presented increased electrochemical activity compared to the retreating one (RS).

https://doi.org/10.21577/0100-4042.20230014

Hepatozoon Miller, 1908 parasites in the Colubridae snakes Clelia clelia (Daudin, 1803) and Drymarchon corais (Boie, 1827) from the Eastern Amazonia
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PICELLI, AMANDA MARIA

; SILVA, MARIA REGINA L.

; CORREA, JAMILLE KARINA C.

; PAIVA, GLEICIERLE R.

; PAULA, FABIANE R.

; HERNÁNDEZ-RUZ, EMIL JOSÉ

; OLIVEIRA, ELCIOMAR A.

; VIANA, LÚCIO ANDRÉ

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220115

Resumo: En | Texto: En | PDF: En

Abstract Based on the genetic, morphological, and morphometric data of blood gamonts, we identified Hepatozoon parasites in colubrid snakes sampled in the Eastern Amazon region. Hepatozoon trigeminum was detected in the mussurana snake Clelia clelia and exhibited wide and elongated gamonts (mean dimensions: 14.25±0.65 × 4.31±0.43 μm) with an evident parasitophorous vacuole. Hepatozoon odwyerae sp. nov. was described in the indigo snake Drymarchon corais, whose gamonts have elongated and thin bodies (mean dimensions: 13.41±0.79 × 3.72±0.35 μm) with one end more tapered than the other. Phylogenetic analyses, based on the amplification of a 441 bp fragment of the 18S rRNA gene, revealed that the novel sequences of Hepatozoon spp. from our study were closely related to hemogregarine lineages found in lizards and snakes from Brazil, forming a well-supported monophyletic clade with them. The present study provides the first species description of Hepatoozon in D. corais and a new record of a host species for C. clelia using the integrated taxonomic data. We also highlight the importance of further investigations into the diversity of Hepatozoon in snakes, a rich but underestimated group of parasites, especially in the Amazonian biome.

https://doi.org/10.1590/0001-3765202320220115

10.

Healthcare workers exposed to COVID-19 patients present an inflammatory status and Th2/Th17/Th22 immune profile: findings from before vaccine application in Brazil
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AQUINO, RODRIGO CESAR A. DE

; BARROS, BÁRBARA RAFAELA S.

; SILVA, GUILHERME ANTONIO S.

; SOUSA, GEORON F. DE

; SOUZA, EDSON B. DE

; SILVA, DYEGO R.C.

; NASCIMENTO, ARIONE V. DO

; SÁ, IGOR W.A. DE

; LIMA, ELKER L.S. DE

; SILVA, BÁRBARA O.

; LIMA, LUÍSA P.O. DE

; VIEIRA, AMANDA M.

; BARBOSA NETO, ADAUTO G.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; OLIVEIRA, TALITA HELENA ARAÚJO DE

; CARVALHO, BRUNO M.

; MUNIZ, MARIA TEREZA C.

; FREITAS, ANTONIO CARLOS DE

; CAMPELO JÚNIOR, EVÔNIO B.

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20220502

Resumo: En | Texto: En | PDF: En

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

11.

Imputation of precipitation data in northeast Brazil
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RODRIGUES, DANIELE T.

; GONÇALVES, WEBER A.

; SILVA, CLÁUDIO MOISÉS S. E

; SPYRIDES, MARIA HELENA C.

; LÚCIO, PAULO SÉRGIO

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Nº 2 elocation e20210737

Resumo: En | Texto: En | PDF: En

Abstract This article evaluates four statistical methods of multiple imputation to fill in the missing data of daily precipitation in Northeast Brazil (NEB). We used a daily database collected by 94 rain gauges distributed in NEB from January 1, 1986 to December 31, 2015. The methods were: random sampling from the observed values; predictive mean matching, Bayesian linear regression; and bootstrap expectation maximization algorithm (BootEm). To compare these methods, missing data from the original series were initially excluded. The next step was to create three scenarios for each method, in which 10\%, 20\% and 30\% of the data were removed at random. The BootEM method presented the best statistical results. With the average bias between the complete series and the imputed series values ranging between -0.91 and 1.30 mm/day. The values of the Pearson correlation ranging between 0.96, 0.91 and 0.86 respectively for 10\%, 20\% and 30\% missing data. We conclude that this is an adequate method for the reconstruction of historical precipitation data in NEB.

https://doi.org/10.1590/0001-3765202320210737

12.

Deep sea ecosystem exploration and ‘health check’: sampling strategy and methods applied during the iAtlantic_BR10_Petrobras cruise in the Santos Basin, Southwest Atlantic
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Perez, Jose Angel A.

; Barros Neto, Halésio M.C. de

; Arantes, Renata C.M.

; Gaurisas, Daniela Y.

; Silva, Camila F.

; Alves, Fernanda Maria M.

; Costa, Júlia A.

; Eloi, Paula D.C.

; Fonseca, Thayse S.

; Gavazzoni, Lucas

; Lonskis, Isabella da S.

; Nardi, Ricardo U.

; Nascimento, Pedro O.

; Rodrigues, João Vitor M.

; Santos, Ana Lara F. dos

; Santos, Edson C. dos

; Schroeder, Rafael

; Silva, Luis Carlos da

; Souza, Fernanda dos S.S. de

; Bernardino, Ângelo F.

; Cavalcanti, Guarani H.

; Lindner, Alberto

; Mahiques, Michel M. de

; Millo, Christian

; Reis, Priscila

; Sweetman, Andrew K.

; Roberts, J. Murray

Ocean and Coastal Research

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2023, Volume 71 elocation e23046

Resumo: En | Texto: En | PDF: En

Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

13.

Different stocks of Prochilodus lineatus along the Cinzas River basin: contributions from a free-flowing tributary in the Capivara Reservoir region, Paranapanema River basin (upper Paraná River) freeflowing free flowing region upper
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Ferreira, Dhiego G.

; Galindo, Bruno A.

; Limeira, Daniel M.

; Garla, Alanna

; Marques, Ana J. C.

; Moreira, Amanda A.

; Rodrigues, Maria V. H.

; Clemente, Roberta C.

; Shimpo, Jhennifer Y.

; Martins, Gabriel A.

; Almeida, Fernanda S.

; Apolinário-Silva, Caroline

; Zanatta, Augusto S.

; Sofia, Silvia H.

; Souza-Shibatta, Lenice

Neotropical Ichthyology

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2023, Volume 21 Nº 4 elocation e230080

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Barragens são umas das principais ameaças para peixes potamódromos Neotropicais, causando bloqueios de rotas migratórias e perdas de áreas de reprodução em um número crescente de sistemas de drenagem. Neste cenário, dados atuais defendem que a manutenção de tributários de fluxo livre em bacias hidrográficas fragmentadas seria uma importante estratégia na conservação de peixes migradores, tanto em situações de panmixia quanto em cenários com diferentes estoques coexistindo, tal como já relatado para Prochilodus lineatus. Este estudo analisou locos microssatélites e haplótipos mitocondriais em amostras de P. lineatus obtidas em diferentes anos ao longo da bacia do rio das Cinzas, no reservatório de Capivara (maior reservatório na calha principal do rio Paranapanema, alto rio Paraná) e no rio Tibagi, todos conectados por trechos livres, visando investigar as contribuições da bacia do rio das Cinzas na dinâmica populacional de peixes migradores na região do reservatório de Capivara. Ambos os marcadores detectaram altos níveis de diversidade genética e indicaram diferentes estoques de P. lineatus ao longo da bacia do rio das Cinzas, sugerindo alguma estruturação genética temporal, bem como a mistura destes estoques na amostra do reservatório de Capivara e de outros pontos, corroborando as relações de estoques neste reservatório e seus tributários. Neotropicais drenagem cenário coexistindo P maior Paranapanema Paraná Tibagi livres temporal pontos

Abstract Dams are one of the main threats to Neotropical potamodromous fish, causing blockages of migratory routes and loss of breeding areas in an increasing number of drainage systems. In this scenario, current data argue that the maintenance of free-flowing tributaries along fragmented watersheds would be an important strategy to the migratory fish conservation, both in panmixia situations and in scenarios with different coexisting stocks, as already reported for Prochilodus lineatus. This study analyzed microsatellite loci and mitochondrial haplotypes in samples of P. lineatus obtained in different years along the Cinzas River basin, as well as in the Capivara Reservoir (largest Reservoir in the Paranapanema main channel, upper Paraná River) and in the Tibagi River, all connected by free stretches, aiming to investigate the contributions of the Cinzas River basin to the population dynamics of migratory fish in the Capivara reservoir region. Both markers detected high genetic diversity levels and indicated different genetic stocks of P. lineatus along the Cinzas River basin, suggesting some temporal genetic structuring, as well as the mixture of these stocks in the sample from the Capivara reservoir and from other points, corroborating the relationships among stocks in this reservoir and its tributaries. systems scenario freeflowing flowing conservation P largest channel stretches region structuring points

https://doi.org/10.1590/1982-0224-2023-0080

14.

First finding of the tick Amblyomma tigrinum parasitizing a Molina’s hog-nosed skunk, Conepatus chinga (Molina 1782), in South America
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Manqui, Jonatan

; Hernández, Felipe A.

; Silva-De LaFuente, María C.

; Acosta-Jamett, Gerardo

Gayana (Concepción)

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Dez 2022, Volume 86 Nº 2 Páginas 59 - 64

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Adult individuals of Amblyomma tigrinum were collected for the first time from a Molina’s hog-nosed skunk (Conepatus chinga), captured in a rural area of north-central Chile, which were described and identified morphologically. There are scarce studies that address the role of the skunk as ectoparasite host; however, the great plasticity of A. tigrinum to inhabit different environments and its strict to moderate specificity for Carnivora order suggest this mephitid as a potentially suitable host for this tick species.

RESUMEN Se colectan por primera vez individuos adultos de Amblyomma tigrinum en un chingue (Conepatus chinga), capturado en área rural del norte-centro de Chile, los cuales fueron descritos e identificados morfológicamente. Existen escasos estudios que aborden el rol del chingue como hospedador de ectoparásitos; sin embargo, la gran plasticidad de A. tigrinum para habitar diferentes ambientes y su especificidad estricta a moderada por el orden Carnivora sugieren a este mefítido como un hospedador potencialmente adecuado para esta especie de garrapata.

15.

Preparation of Silicon Oxycarbide (SiCO) Ceramics from Different Polymer Architectures and Assessment on the Performance as Electrodic Materials for Voltammetric Sensing of Antioxidant Phenolic Compounds
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Silva, Lívia R. C.

; Silva, Maria A.

; Silva, Paulo Rogério C. da

; Tarley, César Ricardo T.

; Segatelli, Mariana G.

Journal of the Brazilian Chemical Society

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Dez 2022, Volume 33 Nº 12 Páginas 1469 - 1484

Resumo: En | Texto: En | PDF: En

Silicon oxycarbide (SiCO) ceramics obtained from polymer pyrolysis and with varied carbon contents were prepared and evaluated as new electrodic materials for sensing of tert butylhydroquinone (TBHQ) and butylated hydroxyanisole (BHA) antioxidants. Three polymers were synthesized varying molecular architecture and aromatic carbon groups, followed by pyrolysis at 1000 and 1500 °C to obtain SiCO ceramics. Graphitization and crystallization processes were evidenced, in different extent, according to precursor chemistry. SiCO at 1500 °C with intermediary carbon content provided slight improvement in the peak anodic separation of TBHQ and BHA and higher peak current compared with glassy carbon electrode (GCE). Higher acidic sites concentration together with more ordered residual carbon phase produced in situ contributed to great electrochemical performance of SiCO towards phenolic compounds detection. Therefore, the ceramic herein prepared exhibits highly efficient features as a new SiCO composited electrode for simultaneous determination of antioxidant phenolic compounds bearing in mind further analytical applications.

https://doi.org/10.21577/0103-5053.20220081

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