Pesquisa | SciELO

ABSTRACT Aedes (Stegomyia) albopictus (Skuse, 1854) was reported in Brazil for the first time in 1986 and has shown marked expansion throughout the Brazilian territory. During a routine activity to control dengue fever conducted by the Division of Entomology of the Municipal Health Department in Rio Branco city, adults and immatures of Culicidae were collected in a peri-urban area. The identified Culicidae forms indicated that they belonged to the species Ae. albopictus. This is the first official record of the presence of Ae. albopictus in the Acre State, confirming its current presence in all Brazilian states.

https://doi.org/10.1590/s1678-9946202365020

Self-pollination, intra- and interspecific crosses in tomatoes
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Zeist, André Ricardo

; Resende, Juliano Tadeu Vilela de

; da-Silva, Paulo Roberto

; Maluf, Wilson Roberto

; Silva Júnior, André Dutra

; Lima Filho, Renato Barros de

; Faria, Marcos Ventura

Scientia Agricola

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2023, Volume 80 elocation e20220016

Resumo: En | Texto: En | PDF: En

ABSTRACT Tomato genotypes ( Solanum spp.) have genetic variability of most desirable features, such as resistance to biotic and abiotic stresses. However, incompatibility of crosses of wild genotypes with domesticated tomatoes, or even between wild genotypes, hinders the breeding process. Thus, knowledge of the reproductive biology of genotypes and conditions is necessary to maximize the success of artificial crossings. This study evaluated the compatibility of self-pollination, intra- and interspecific controlled crosses, stigma receptivity, and pollen viability in tomato genotypes. We used two commercial genotypes S. lycopersicum (‘RVTM08’ and ‘Redenção’) and seven accessions of wild tomato genotypes (‘AF 26970’, ‘LA-1401’, ‘AF 19684’, ‘LA-1967’, ‘PI-127826’, ‘PI-134417’, and ‘LA-716’). We evaluated all crosses and their reciprocals, besides the self-pollinations. The variables evaluated were fruit index (FI), number of seeds per fruit (SN), and seed germination percentage (GP). Stigma receptivity and grains’ pollen viability index (PVI) were also assessed. The results showed that ‘LA-1967’ was self-incompatible, had a low PVI, and generated fruit without seeds in most crosses. As female parents, ‘RVTM08’, ‘Redenção’, ‘AF 26970’, ‘LA-1401’, and ‘AF 19684’ showed higher FI and SN. There was a wide diversity of reproductive characteristics between the genotypes and crosses that did not influence GP. Compatibility of crosses in tomatoes is determined by the female parent choice and can be affected by stigma receptivity and the PVI.

https://doi.org/10.1590/1678-992x-2022-0016

Diretriz Brasileira de Dispositivos Cardíacos Eletrônicos Implantáveis – 2023
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Teixeira, Ricardo Alkmim

; Fagundes, Alexsandro Alves

; Baggio Junior, José Mário

; Oliveira, Júlio César de

; Medeiros, Paulo de Tarso Jorge

; Valdigem, Bruno Pereira

; Teno, Luiz Antônio Castilho

; Silva, Rodrigo Tavares

; Melo, Celso Salgado de

; Elias Neto, Jorge

; Moraes Júnior, Antonio Vitor

; Pedrosa, Anisio Alexandre Andrade

; Porto, Fernando Mello

; Brito Júnior, Hélio Lima de

; Souza, Thiago Gonçalves Schroder e

; Mateos, José Carlos Pachón

; Moraes, Luis Gustavo Belo de

; Forno, Alexander Romeno Janner Dal

; D’Avila, Andre Luiz Buchele

; Cavaco, Diogo Alberto de Magalhães

; Kuniyoshi, Ricardo Ryoshim

; Pimentel, Mauricio

; Camanho, Luiz Eduardo Montenegro

; Saad, Eduardo Benchimol

; Zimerman, Leandro Ioschpe

; Oliveira, Eduardo Bartholomay

; Scanavacca, Mauricio Ibrahim

; Martinelli Filho, Martino

; Lima, Carlos Eduardo Batista de

; Peixoto, Giselle de Lima

; Darrieux, Francisco Carlos da Costa

; Duarte, Jussara de Oliveira Pinheiro

; Galvão Filho, Silas dos Santos

; Costa, Eduardo Rodrigues Bento

; Mateo, Enrique Indalécio Pachón

; Melo, Sissy Lara De

; Rodrigues, Thiago da Rocha

; Rocha, Eduardo Arrais

; Hachul, Denise Tessariol

; Lorga Filho, Adalberto Menezes

; Nishioka, Silvana Angelina D’Orio

; Gadelha, Eduardo Barreto

; Costa, Roberto

; Andrade, Veridiana Silva de

; Torres, Gustavo Gomes

; Oliveira Neto, Nestor Rodrigues de

; Lucchese, Fernando Antonio

; Murad, Henrique

; Wanderley Neto, José

; Brofman, Paulo Roberto Slud

; Almeida, Rui M. S.

; Leal, João Carlos Ferreira

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 1 elocation e20220892

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

https://doi.org/10.36660/abc.20220892

Overview of Q fever in Brazil: an underestimated zoonosis Brazil
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França, Danilo Alves de

; Mioni, Mateus de Souza Ribeiro

; Fernandes, Jorlan

; Lemos, Elba Regina Sampaio de

; Duré, Ana Íris de Lima

; Silva, Marcos Vinicius Ferreira

; Langoni, Helio

; Megid, Jane

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volume 65 elocation e39

Resumo: En | Texto: En | PDF: En

ABSTRACT This review aims to provide current information about Q fever, elucidating the etiological, epidemiological, pathogenic, clinical, diagnostic, therapeutic, and prophylactic aspects of the disease for the medical community. We discuss the main forms of presentation of the agent, its ability to persist in the body, the infinite possibilities of susceptible hosts, the main known forms of transmission, its importance in populations at occupational risk, and the role of arthropods in the natural history of the disease. Focusing on Brazil, we present the cases already described and studies developed since its first report, and how there is still much to unravel. We are aware of the possibilities of the persistence of the agent and the development of severe clinical pictures and the specific treatments currently instituted. We also wish to raise awareness about the future, the new genotypes that are emerging, the need to study the effects of vaccines, and the impact of Q fever on the population. Q fever is a poorly understood disease in Latin America, and recent studies, especially in Brazil, have revealed the importance of developing new studies. etiological epidemiological pathogenic diagnostic therapeutic community body hosts transmission risk Brazil report unravel instituted future emerging vaccines population America

https://doi.org/10.1590/s1678-9946202365039

AN ADAPTED UNMANNED AERIAL VEHICLE FOR ENVIRONMENTAL WATER SAMPLING
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D. Neto, José C.

; Resque, Ian S.

; Avelino, Rodrigo A.

; Santos, Vagner B. dos

; Leite, Lucas S.

; Cesar, Lucas O.

; Belo, Paulo H. da Silva

; Albuquerque, Jones

; Lima Filho, José L. de

Química Nova

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Jun 2022, Volume 45 Nº 6 Páginas 734 - 741

Resumo: En | Texto: En | PDF: En

In this work an unmanned aerial vehicle (UAV) was adapted to be used as an environmental tool to collect water samples of sea, estuary, and mangrove for further analysis by gas chromatography with a mass detector (GC-MS). The sampling automated system was based on a microcontroller, a solenoid valve, and a peristaltic mini-pump that was coupled to a quadcopter UAV. The apparatus was properly calibrated to acquire 100 mL of environmental water at 4.6 min. After, the concentration of BTEX (benzene, toluene, ethylbenzene, and o, m, p-xylenes) was analyzed in laboratory using GC-MS and the BTEX concentration ranged from 0.3 to 5.0 µg L-1 with a recovery from 83.9 to 118% with a relative standard deviation lower than 7.0%. Tests of cross-contamination were performed by simultaneous sampling and laboratory blank was carried out and no contamination was found for BTEX analysis. Based on the results, the adapted UAV presented a very useful ability to collect samples in difficult access areas such as mangroves, points between rocks, corals reefs, without health risk and without impacting the fragile ecosystem being an interesting tool for environmental purposes.

https://doi.org/10.21577/0100-4042.20170892

Adipose tissue-derived stromal/stem cells + cholecalciferol: a pilot study in recent-onset type 1 diabetes patients
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Dantas, Joana Rodrigues

; Araújo, Débora Batista

; Silva, Karina Ribeiro

; Souto, Débora Lopes

; Pereira, Maria de Fátima Carvalho

; Luiz, Ronir Raggio

; Mantuano, Matheus dos Santos

; Claudio-da-Silva, Cesar

; Gabbay, Monica Andrade Lima

; Dib, Sérgio Atala

; Couri, Carlos Eduardo Barra

; Maiolino, Angelo

; Rebelatto, Carmen Lúcia Kuniyoshi

; Daga, Débora Regina

; Senegaglia, Alexandra Cristina

; Brofman, Paulo Roberto Slud

; Baptista, Leandra S.

; Oliveira, José Egídio Paulo de

; Zajdenverg, Lenita

; Rodacki, Melanie

Archives of Endocrinology and Metabolism

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Jun 2021, Volume 65 Nº 3 Páginas 342 - 351

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective: Adipose tissue-derived stromal/stem cells (ASCs) and vitamin D have immunomodulatory actions that could be useful for type 1 diabetes (T1D). We aimed in this study to investigate the safety and efficacy of ASCs + daily cholecalciferol (VIT D) for 6 months in patients with recent-onset T1D. Materials and methods: In this prospective, dual-center, open trial, patients with recent onset T1D received one dose of allogenic ASC (1 x 106 cells/kg) and cholecalciferol 2,000 UI/day for 6 months (group 1). They were compared to patients who received chol-ecalciferol (group 2) and standard treatment (group 3). Adverse events were recorded; C-peptide (CP), insulin dose and HbA1c were measured at baseline (T0), after 3 (T3) and 6 months (T6). Results: In group 1 (n = 7), adverse events included transient headache (all), mild local reactions (all), tachycardia (n = 4), abdominal cramps (n = 1), thrombophlebitis (n = 4), scotomas (n = 2), and central retinal vein occlusion at T3 (n = 1, resolution at T6). Group 1 had an increase in basal CP (p = 0.018; mean: 40.41+/-40.79 %), without changes in stimulated CP after mixed meal (p = 0.62), from T0 to T6. Basal CP remained stable in groups 2 and 3 (p = 0.58 and p = 0.116, respectively). Group 1 had small insulin requirements (0.31+/- 0.26 UI/kg) without changes at T6 (p = 0.44) and HbA1c decline (p = 0.01). At T6, all patients (100%; n = 7) in group 1 were in honeymoon vs 75% (n = 3/4) and 50% (n = 3/6) in groups 2 and 3, p = 0.01. Conclusions: Allogenic ASC + VIT D without immunosuppression was safe and might have a role in the preservation of β-cells in patients with recent-onset T1D. ClinicalTrials.gov: NCT03920397.

https://doi.org/10.20945/2359-3997000000368 236 downloads

COVID-19: The question of genetic diversity and therapeutic intervention approaches
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Figueiredo, David Livingstone Alves

; Ximenez, João Paulo Bianchi

; Seiva, Fábio Rodrigues Ferreira

; Panis, Carolina

; Bezerra, Rafael dos Santos

; Ferrasa, Adriano

; Cecchini, Alessandra Lourenço

; Medeiros, Alexandra Ivo de

; Almeida, Ana Marisa Fusco

; Ramão, Anelisa

; Boldt, Angelica Beate Winter

; Moya, Carla Fredrichsen

; Chin, Chung Man

; Paula, Daniel de

; Rech, Daniel

; Gradia, Daniela Fiori

; Malheiros, Danielle

; Venturini, Danielle

; Tavares, Eliandro Reis

; Carraro, Emerson

; Ribeiro, Enilze Maria de Souza Fonseca

; Pereira, Evani Marques

; Tuon, Felipe Francisco

; Follador, Franciele Aní Caovilla

; Fernandes, Glaura Scantamburlo Alves

; Volpato, Hélito

; Cólus, Ilce Mara de Syllos

; Oliveira, Jaqueline Carvalho de

; Rodrigues, Jean Henrique da Silva

; Santos, Jean Leandro dos

; Visentainer, Jeane Eliete Laguila

; Brandi, Juliana Cristina

; Serpeloni, Juliana Mara

; Bonini, Juliana Sartori

; Oliveira, Karen Brajão de

; Fiorentin, Karine

; Lucio, Léia Carolina

; Faccin-Galhardi, Ligia Carla

; Ferreto, Lirane Elize Defante

; Lioni, Lucy Megumi Yamauchi

; Consolaro, Marcia Edilaine Lopes

; Vicari, Marcelo Ricardo

; Arbex, Marcos Abdo

; Pileggi, Marcos

; Watanabe, Maria Angelica Ehara

; Costa, Maria Antônia Ramos

; Giannini, Maria José S. Mendes

; Amarante, Marla Karine

; Khalil, Najeh Maissar

; Lima Neto, Quirino Alves de

; Herai, Roberto H.

; Guembarovski, Roberta Losi

; Shinsato, Rogério N.

; Mainardes, Rubiana Mara

; Giuliatti, Silvana

; Yamada-Ogatta, Sueli Fumie

; Gerber, Viviane Knuppel de Quadros

; Pavanelli, Wander Rogério

; Silva, Weber Claudio da

; Petzl-Erler, Maria Luiza

; Valente, Valeria

; Soares, Christiane Pienna

; Cavalli, Luciane Regina

; Silva Jr, Wilson Araujo

Genetics and Molecular Biology

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2021, Volume 44 Nº 1 elocation e20200452

Resumo: En | Texto: En | PDF: En

Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2), is the largest pandemic in modern history with very high infection rates and considerable mortality. The disease, which emerged in China’s Wuhan province, had its first reported case on December 29, 2019, and spread rapidly worldwide. On March 11, 2020, the World Health Organization (WHO) declared the COVID-19 outbreak a pandemic and global health emergency. Since the outbreak, efforts to develop COVID-19 vaccines, engineer new drugs, and evaluate existing ones for drug repurposing have been intensively undertaken to find ways to control this pandemic. COVID-19 therapeutic strategies aim to impair molecular pathways involved in the virus entrance and replication or interfere in the patients’ overreaction and immunopathology. Moreover, nanotechnology could be an approach to boost the activity of new drugs. Several COVID-19 vaccine candidates have received emergency-use or full authorization in one or more countries, and others are being developed and tested. This review assesses the different strategies currently proposed to control COVID-19 and the issues or limitations imposed on some approaches by the human and viral genetic variability.

https://doi.org/10.1590/1678-4685-gmb-2020-0452

Organic fertilization in the production and chemical composition of non-conventional leafy vegetables
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Batista, Deborah Sepini

; Mantovani, José Ricardo

; Silva, Luis Felipe Lima e

; Souza, Douglas Correa de

; Landgraf, Paulo Roberto Corrêa

Pesquisa Agropecuária Tropical

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2021, Volume 51 elocation e66508

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO As hortaliças folhosas não convencionais podem ser uma boa fonte de nutrientes para a dieta humana, mas há poucas informações sobre adubação para essas espécies. Objetivou-se avaliar o efeito da adubação orgânica com esterco bovino na produção e composição química de hortaliças folhosas não convencionais. O experimento foi conduzido em casa-de-vegetação, em vasos. Os fatores em estudo foram espécies de hortaliças não convencionais (Rumex acetosa, Stachys byzantina e Lactuca canadensis) e doses de esterco bovino (0; 5; 10; 15; e 20 t ha-1), arranjados em esquema fatorial 3 x 5, em delineamento de blocos ao acaso, com quatro repetições. Na colheita, foram avaliados o diâmetro de caule, número total de folhas, número de folhas com padrão comercial, matéria fresca e seca da parte aérea, concentração e quantidades acumuladas de macro e micronutrientes na parte aérea. A adubação com esterco bovino alterou o diâmetro de caule, número de folhas com padrão comercial, matéria fresca e seca da parte aérea, bem como o acúmulo dos nutrientes N, P, K, Ca, Fe, Mn e Zn na parte aérea das três espécies estudadas. Os acréscimos obtidos variaram de 1,1 a 2,1 vezes, em relação ao tratamento sem adubação orgânica, e a dose equivalente a 20 t ha-1 foi a mais adequada. A adubação orgânica com esterco bovino aumenta a produção e o acúmulo de nutrientes nas hortaliças não convencionais avaliadas, sendo que R. acetosa apresenta maior acúmulo de nutrientes do que S. byzantina e L. canadensis.

ABSTRACT Non-conventional leafy vegetables can be a good source of nutrients for the human diet, but there is little information on fertilization for these species. This study aimed to evaluate the effect of organic fertilization with cattle manure on the production and chemical composition of non-conventional leafy vegetables. The experiment was conducted in a greenhouse, in pots. The factors under study were non-conventional leafy species (Rumex acetosa, Stachys byzantina and Lactuca canadensis) and cattle manure doses (0; 5; 10; 15; and 20 t ha-1), arranged in a 3 x 5 factorial scheme, in a randomized block design, with four replications. At harvest, the following parameters were evaluated: stem diameter; total number of leaves; number of leaves with commercial standard; shoot fresh and dry matter; macro and micronutrients concentration and accumulated amounts in the shoot. The fertilization with cattle manure altered the stem diameter, number of leaves with commercial standard, shoot fresh and dry matter, as well as the accumulation of the nutrients N, P, K, Ca, Fe, Mn and Zn in the shoot of the three species studied. The additions obtained varied from 1.1 to 2.1 times, in relation to the treatment without organic fertilization, and the dose equivalent to 20 t ha-1 was the most adequate one. The organic fertilization with cattle manure increases the production and accumulation of nutrients in the non-conventional vegetables evaluated, with R. acetosa presenting a nutrient accumulation higher than S. byzantina and L. canadensis.

https://doi.org/10.1590/1983-40632021v5166508 178 downloads

Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016)
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Silva-Sales, Marcelle

; Leal, Elcio

; Milagres, Flavio Augusto de Pádua

; Brustulin, Rafael

; Morais, Vanessa dos Santos

; Marcatti, Roberta

; Araújo, Emerson Luiz Lima

; Witkin, Steven S.

; Deng, Xutao

; Sabino, Ester Cerdeira

; Delwart, Eric

; Luchs, Adriana

; Costa, Antonio Charlys da

Revista do Instituto de Medicina Tropical de São Paulo

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2020, Volume 62 elocation e98

Resumo: En | Texto: En | PDF: En

ABSTRACT Surveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins State (Northern Brazil) during six years of post-vaccination follow-up (2010-2016). A total of 248 stool samples were screened by next generation sequencing and 107 (43.1%) nearly full length RVA genome sequences were obtained; one sample was co-infected with two RVA strains (G2/G8P[4]). Six G and P genotypes combinations were detected: G12P[8] strains (78.6%), as well as the G3P[8] (9.3%) and G1P[8] (0.9%) were associated with a Wa-like genogroup backbone. All G2P[4] (5.6%) and G8P[4] (2.8%) strains, including the mixed G2/G8P[4] infection (0.9%) showed the DS-1-like genetic background. The two G12P[4] strains (1.9%) were associated with distinct genetic backbones: Wa-like and DS-1-like. The phylogenetic analysis revealed the circulation of lineages G1-I, G2-IV, G3-III, G8-I and G12-III, and P[4]-V and P[8]-III of the VP7 and VP4 genes, respectively. Conserved clustering pattern and low genetic diversity were observed regarding VP1-VP3 and VP6, as well as NSP1-5 segments. We identified the same RVA circulation pattern reported in other Brazilian regions in the period of 2010-2016, suggesting that rural and low-income areas may not have a different RVA genotypic distribution compared to other parts of the country. The unique presentation of whole-genome data of RVA strains detected in the Tocantins State provides a baseline for monitoring variations in the genetic composition of RVA in this area.

https://doi.org/10.1590/s1678-9946202062098 434 downloads

10.

Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata
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Queiroz, Fábio Ribeiro

; Portilho, Laysa Gomes

; Jeremias, Wander de Jesus

; Babá, Élio Hideo

; do Amaral, Laurence Rodrigues

; Silva, Luciana Maria

; Coelho, Paulo Marcos Zech

; Caldeira, Roberta Lima

; Gomes, Matheus de Souza

Memórias do Instituto Oswaldo Cruz

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2020, Volume 115 elocation e190498

Resumo: En | Texto: En | PDF: En

BACKGROUND Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs have become more relevant. The small RNAs have been considered important through their ability to finely regulate the gene expression in several organisms, thus controlling the functions like cell growth, metabolism, and susceptibility/resistance to infection. OBJECTIVE The present study aims on identification and characterisation of the repertoire of small non-coding RNAs in B. glabrata (Bgl-small RNAs). METHODS By using small RNA sequencing, bioinformatics tools and quantitative reverse transcription polymerase chain reaction (RT-qPCR), we identified, characterised, and validated the presence of small RNAs in B. glabrata. FINDINGS 89 mature miRNAs were identified and five of them were classified as Mollusk-specific. When compared to model organisms, sequences of B. glabrata miRNAs showed a high degree of conservation. In addition, several target genes were predicted for all the mature miRNAs identified. Furthermore, piRNAs were identified in the genome of B. glabrata for the first time. The B. glabrata piRNAs showed strong conservation of uridine as first nucleotide at 5’ end, besides adenine at 10th position. Our results showed that B. glabrata has diverse repertoire of circulating ncRNAs, several which might be involved in mollusk susceptibility to infection, due to their potential roles in the regulation of S. mansoni development. MAIN CONCLUSIONS Further studies are necessary in order to confirm the role of the Bgl-small RNAs in the parasite/host relationship thus opening new perspectives on interference of small RNAs in the organism development and susceptibility to infection.

https://doi.org/10.1590/0074-02760190498 845 downloads

11.

Uso do Arduíno como um sistema alternativo para medir radiação solar global e práticas educacionais
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Alves, Péricles Vale

; Reis, Luis Henrique Souza

; Querino, Carlos Alexandre S.

; Moura, Marcos Antônio Lima

; Feitosa Júnior, Adalcir Araújo

; Martins, Paulo André da Silva

Revista Brasileira de Ensino de Física

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2020, Volume 42 elocation e20190304

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Quantificar a intensidade da radiação solar incidente na superfície terrestre é extremamente importante, pois os processos de ordem natural, sejam eles físicos, químicos e/ou biológicos dependem diretamente dela. Contudo, existem algumas dificuldades para medir esta variável, na qual destaca-se o custo elevado de equipamentos para esta finalidade. Desta forma, o objetivo deste trabalho foi desenvolver um protótipo de baixo custo para medir radiação solar global bem como promover a contextualização do ensino de física. Uma estrutura de alumínio fundido, para minimizar os efeitos térmicos e as intempéries sobre suas medidas foi desenvolvida. Esta estrutura era coberta com um difusor plano de acrílico e uma cúpula semi-esférica de vidro que envolvia o sensor fotoresistivo. O protótipo foi conectado ao arduino, usado como datalogger, através de um cabo de rede. Para a calibração do protótipo foi utilizado um piranômetro comercial modelo CM5 da KIPP & ZONEN. Índices estatísticos para se medir a acurácia do protótipo foram aplicados. As medidas foram realizadas continuamente, a cada minuto, durante cinco dias. Os resultados mostraram: coeficiente de determinação foi de R 2 = 0 , 957, de eficiência ( ε = 0 , 95 ), índice de concordância de Willmott ( d = 0 , 99 ) e o erro médio absoluto ( E M A = 51 , 22 W m − 2 ). Verifica-se uma boa aproximação dos valores de radiação solar global medidas pelo protótipo referentes aos do CM5. Portanto, a construção deste protótipo é útil para facilitar e difundir medidas diárias de radiação solar global e, sobre tudo, poderá contribuir com a contextualização dos conceitos estudados em Física nos ensinos básico e superior.

Abstract Quantifying the intensity of the incoming solar radiation at the earth's surface is important, to the natural, physical, chemical and / or biological processes. However, there are some difficulties in measuring this variable, due to the high cost of equipment for this purpose. Thus, the objective of this work was to develop a low cost prototype to measure global solar radiation. A cast aluminum frame, covered with a flat acrylic diffuser and a semi-spherical glass dome, to surround the photoresist sensor and minimize thermal and weather effects on its measurements, was made. The prototype was connected to the arduino, used as datalogger. A commercial KIPP & ZONEN pyranometer model CM5 was used for calibration. Measurement accuracy was determined by determination coefficients ( R 2 = 0.957 ), coefficients of efficiency ( ε = 0.95 ), Willmott agreement index ( d = 0.99 ), and mean absolute error ( M A E = 51.22 W m − 2 ). There is a good approximation of the global solar radiation values measured by the prototype for CM5. Therefore, the construction of this prototype is useful to facilitate and diffuse daily measurements of global solar radiation and, overall, may contribute to the contextualization of the concepts studied in physics in the highschool and colege education.

https://doi.org/10.1590/1806-9126-rbef-2019-0304 2238 downloads

12.

No correlation was observed between vitamin D levels and disability of patients with multiple sclerosis between latitudes 18° and 30° South
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Fragoso, Yara Dadalti

; Adoni, Tarso

; Alves-Leon, Soniza Vieira

; Apostolos-Pereira, Samira L.

; Arruda, Walter Oleschko

; Brooks, Joseph B. Bidin

; Cal, Henrique S. Rodrigues

; Damasceno, Carlos A. Albuquerque

; Gama, Paulo Diniz

; Goncalves, Marcus V. Magno

; Jesus, Carlos A. da Silva de

; Machado, Suzana C. Nunes

; Mansur, Letícia Fezer

; Matta, Andre P. da Cunha

; Mendes, Maria Fernanda

; Morales, Rogerio Rizo

; Nobrega-Jr, Adaucto Wanderley da

; Parolin, Monica K. Fiuza

; Peres, Mario Pietro

; Ribeiro, Marlise de Castro

; Ruocco, Heloisa Helena

; Scherpenhuijzen, Simone

; Siquinelli, Fabio

; Stoney, Patrick N.

; Varela, Daniel Lima

; Eboni, Audred Cristina Biondo

; Spessotto, Caroline Vieira

; Rocha, Elisa Teixeira da

; Lacerda, Pamela Emanuele

Arquivos de Neuro-Psiquiatria

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Jan 2017, Volume 75 Nº 1 Páginas 3 - 8

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Vitamina D assumiu um papel central na pesquisa e tratamento da esclerose múltipla (EM). O objetivo deste estudo foi avaliar os níveis séricos de vitamina D de pacientes com EM e controles que residem em uma área tropical. Métodos: Foram analisados dados de 535 pacientes com EM e 350 indivíduos controle em 14 cidades próximas ao Trópico de Capricórnio. Resultados: O valor médio da determinação de 25-OH vitamina D foi 26,07 ± 10,27 ng/mL para controles e 28,03 ± 12,19 ng/mL para pacientes com EM. Não houve correlação entre os níveis de vitamina D e o grau de incapacidade ao longo da duração da doença. Conclusão: Pelo menos na região que cerca o Trópico de Capricórnio, os níveis séricos de vitamina D estão entre valores de 20 a 30 ng/mL tanto para controles quanto para pacientes com EM.

ABSTRACT Objective: Vitamin D has taken center stage in research and treatment of multiple sclerosis (MS). The objective of the present study was to assess the serum vitamin D levels of a large population of patients with MS and controls living in a restricted tropical area. Methods: Data from 535 patients with MS and 350 control subjects were obtained from 14 cities around the Tropic of Capricorn. Results: The mean serum 25-OH vitamin D level was 26.07 ± 10.27 ng/mL for the control subjects, and 28.03 ± 12.19 ng/mL for patients with MS. No correlation was observed between vitamin D levels and the disability of patients over the disease duration. Conclusion: At least for the region around the Tropic of Capricorn, serum levels of vitamin D typically are within the range of 20 to 30 ng/mL for controls and patients with MS.

https://doi.org/10.1590/0004-282x20160173 3011 downloads

13.

Avaliação de microrganismos antagônicos, Saccharomyces cerevisiae e Bacillus subtilis para o controle de Penicillium digitatum
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Kupper, Katia Cristina

; Cervantes, Antonio Lucas Lima

; Klein, Mariana Nadjara

; Silva, Aline Caroline da

Revista Brasileira de Fruticultura

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Jun 2013, Volume 35 Nº 2 Páginas 425 - 436

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Os frutos cítricos são afetados por diversas doenças, especialmente as fúngicas, as quais afetam a produtividade e a qualidade, principalmente quando se visa ao mercado de frutas frescas, seja para o mercado interno, seja para a exportação. Dentre as doenças fúngicas que ocorrem na fase de pós-colheita, destaca-se o bolor verde, causado por Penicillium digitatum. As medidas de controle baseiam-se, principalmente, no tratamento de frutos com diferentes combinações de fungicidas no packing-house. Devido às restrições quanto à presença de resíduos de fungicidas em frutos de citros e ao crescente desenvolvimento de linhagens resistentes dos patógenos a tais fungicidas, torna-se necessária a busca de alternativas de controle, como o controle biológico. Portanto, este trabalho teve por objetivos: (i) verificar o efeito antagônico de agentes de controle biológico (ACBs), sendo 06 isolados de Saccharomyces cerevisiae e 13 isolados de Bacillus subtilis contra P. digitatum; (ii) estudar as interações in vitro entre ACBs e o fitopatógeno; (iii) verificar o efeito da integração dos antagonistas com bicarbonato de sódio e cera de carnaúba no controle do bolor verde. Os resultados mostraram que a maioria dos isolados bacterianos e todos os isolados de levedura inibiram o crescimento micelial do fitopatógeno. Somente um isolado de Bacillus subtilis (ACB-84) foi capaz de inibir a germinação de P. digitatum com 72% de inibição, enquanto ACB-K1 e ACB-CR1 (S. cerevisiae) foram os mais eficientes com inibições de 78 e 85,7%, respectivamente; a adição de sacarose (a 0,5%) favoreceu ainda mais a inibição da germinação dos conídios pelos isolados da levedura. Os resultados de controle in vivo mostraram a viabilidade de S. cerevisiae ACB-K1 e ACB-CR1 para o controle de P. digitatum, em frutos de lima-ácida 'Tahiti' e laranja 'Hamlin', respectivamente; a associação de bicarbonato de sódio com agentes de biocontrole não resultou em melhorias no controle curativo do bolor verde; cera de carnaúba (18% de SST) favoreceu a atividade antagonística de S. cerevisiae, e tal efeito dependeu da variedade dos frutos cítricos em estudo e do isolado da levedura utilizado para o biocontrole.

Citrus fruits are affected by diverse diseases, mainly the fungal infections, which affect productivity and quality, especially when it targets the market of fresh fruit. Among the fungal diseases that occur in postharvest, there is the green mold caused by Penicillium digitatum. The control measures are based mainly in the treatment of fruits with different combinations of fungicides in packing-house. Due to restrictions on the presence of residues of fungicides in citrus fruits and the increasing development of resistant strains of pathogens to the fungicide used, it is necessary to search for control alternatives such as biological control. Therefore, this study aimed to: (i) verify the antagonistic effect of biological control agents (BCA), being 13 isolates of Bacillus subtilis and 06 isolates of Saccharomyces cerevisiae against P. digitatum, (ii) study in vitro interactions between pathogen and BCA (iii) determine the effect of integration of antagonists with sodium bicarbonate and carnauba wax in the control of green mold. The results showed that the majority of the isolates, and all yeast isolates inhibited the mycelial growth of the phytopathogen. Only one isolate of B. subtilis (ACB-84) was able to inhibit the germination of P. digitatum (72% of inhibition), whereas ACB-K1 and ACB- CR1 (S. cerevisiae) were the most effective with inhibition from 78 and 85.7% respectively. The addition of sucrose (0.5%) favored the inhibition of conidia germination by the yeast isolates. The results from the in vivo control showed the viability of S. cerevisiae ACB-K1 and ACB-CR1 to control P. digitatum in 'Tahiti' lime fruits and orange 'Hamlin' fruits, respectively. The combination of sodium bicarbonate and biocontrol agents did not result in improvements in the curative control of the green mold. Carnauba wax (18% of TSS) favored the antagonistic activity of S. cerevisiae, and this effect depended on the variety of fruits in the study and of the yeast isolate used for the biocontrol.

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14.

Chemical constituents of methanolic extracts of Jatropha curcas L and effects on Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
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Ribeiro, Sandra Santos

; Silva, Thanany Brasil da

; Moraes, Valéria Regina de Souza

; Nogueira, Paulo Cesar de Lima

; Costa, Emmanoel Vilaça

; Bernardo, Antônio Rogério

; Matos, Andreia Pereira

; Fernandes, Batista

; Silva, Maria Fátima das Graças Fernandes da

; Pessoa, Ângela Maria dos Santos

; Silva-Mann, Renata

Química Nova

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2012, Volume 35 Nº 11 Páginas 2218 - 2221

Resumo: En | Texto: En | PDF: En

The biological activity of seven extracts from leaves of different Jatropha curcas L (Euphorbiaceae) accessions was evaluated on Spodoptera frugiperda. Methanol extracts were incorporated into an artificial diet and offered to the larval stage of S. frugiperda. The parameters evaluated were length of larval and pupal stages, mortality of larval and total cycle stage, and weight of pupae. The extracts of the EMB accessions showed the best result for larval mortality at 60.00 and 56.67%, compared with the control, respectively. Hexane partition of the methanol extract of the leaves of PM-14 accessions allowed the identification of phytosterols, phytol and n-alkanols.

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15.

Characterization of Meloidogyne incognita populations from São Paulo and Minas Gerais state and their pathogenicity on coffee plants
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Oliveira, Dagoberto S.

; Oliveira, Rosângela D'Arc Lima

; Silva, Débora G.

; Silva, Rodrigo V.

Tropical Plant Pathology

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Jun 2011, Volume 36 Nº 3 Páginas 190 - 194

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

No Brasil, Meloidogyne incognita é considerado um dos fitonematóides mais agressivos e prejudiciais ao cafeeiro. Entretanto, populações desse nematóide, presentes em Minas Gerais, são incapazes de infectar esse hospedeiro. O objetivo deste trabalho foi caracterizar e avaliar a penetração e o desenvolvimento pós-infectivo de populações de M. incognita que diferem quanto à capacidade de infectar o cafeeiro. As duas populações foram confirmadas como M. incognita pelo fenótipo isoenzimático de esterase, I1, e PCR específico. Foram classificadas como raça 2 pela reação dos hospedeiros diferenciadores. Mudas de cafeeiro suscetível e resistente foram inoculadas com juvenis de segundo estádio (J2) de cada população e avaliadas quanto à penetração e desenvolvimento do nematóide a partir do segundo até o quadragésimo dia após a inoculação. A penetração de J2 da população de M. incognita de São Paulo foi maior do que a da população de Minas Gerais, tanto em cafeeiro suscetível (C. arabica 'Catuaí Vermelho IAC 44') quanto resistente (C. canephora 'Apoatã IAC 2258'). Como esperado, ocorreu maior penetração de J2 das duas populações de M. incognita no cafeeiro suscetível. Nas avaliações posteriores, somente foi observado desenvolvimento pós-infectivo nos indivíduos pertencentes à população de M. incognita de São Paulo em cafeeiro suscetível. Os mecanismos que conferiram incompatibilidade entre as populações de M. incognita de Minas Gerais e o cafeeiro atuaram principalmente na fase de penetração, mas também foi acompanhada pela ação de fatores de resistência pós-penetração que ocasionaram uma significativa emigração de J2 e impediram o estabelecimento do nematóide.

Meloidogyne incognita is one of the most aggressive and harmful plant-parasitic nematodes attacking coffee plantations in Brazil. However, populations from Minas Gerais state (MG) do not incite disease on coffee plants as strongly as populations from São Paulo state (SP). This study aimed to compare the capacity to incite disease on coffee plants from SP and MG-populations based on penetration and post-infective development of second-stage juveniles (J2) stage. Both populations were confirmed as M. incognita by using esterase phenotype I1 and species-specific PCR. Physiologically they were classified as race 2 by differential host test. Susceptible (C. arabica 'Catuaí Vermelho IAC 44') and resistant coffee seedlings (C. canephora 'Apoatã IAC 2258') were inoculated with J2 and assessed for penetration and development from 2nd to 40th day after inoculation. Although the penetration rate of the J2 from both populations was higher in susceptible than in resistant seedlings, the SP-population showed a higher penetration than the MG-population for both variables. Post-infective development proceeded only in individuals of the SP-population in susceptible seedlings. The incompatibility between the MG-population and coffee seedlings was evident at the penetration phase, which was also followed by post-penetration resistance factors leading to significant J2 emigration, impeding nematode establishment.

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